Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| susceptibility of recent bacterial isolates to cefdinir and selected antibiotics among children with urinary tract infections. | cefdinir, an extended-spectrum cephalosporin administered orally, is approved by the u.s. federal drug administration for treatment of skin and respiratory tract infections. during the last two years at the authors' institution, this agent has been used as an off-label treatment for urinary tract infections in children. | 2006 | 16365328 |
| detection of diverse bacterial signatures in atherosclerotic lesions of patients with coronary heart disease. | bacterial infection has been discussed as a potential etiologic factor in the pathophysiology of coronary heart disease (chd). this study analyzes molecular phylogenies to systematically explore the presence, frequency, and diversity of bacteria in atherosclerotic lesions in patients with chd. | 2006 | 16490835 |
| quantitative bacterial micro-assay for rapid detection of serum phenylalanine on dry blood-spots: application in phenylketonuria screening. | phenylketonuria is an inherited metabolic disease, which is characterized by an increased level of serum phenylalanine. quantitative measurement of phenylalanine in the serum of phenylketonuria patients is necessary to confirm the disease, and to distinguish phenylketonuria from other forms of hyperphenylalaninemia. in this study, we report a rapid and inexpensive micro-assay for simultaneous detection and quantitative measurement of serum phenylalanine on dry blood-spots. analysis of the standa ... | 2006 | 16375590 |
| [antimicrobial susceptibility of aerobic bacteria isolated from leishmaniotic ulcers in corte de pedra, ba]. | a prospective study regarding aspects of antimicrobial susceptibility aspects was realized among patients with tegumentary leishmaniasis in corte de pedra, bahia. cases were composed mainly of adolescent and adult farmer patients with single lesions. staphylococcus aureus predominated (83%) in the cultures with susceptibility to the majority of antibiotics. a mixed bacterial flora in ulcers was encountered in 37 (44.1%) patients. among the gram-negative bacteria isolated, enterobacter sp (13.1%) ... | 2006 | 16501766 |
| choice of antibiotic for empirical therapy of acute cystitis in a setting of high antimicrobial resistance. | a high prevalence of antimicrobial resistance among urinary isolates in the garhwal region of uttaranchal. | 2006 | 16505574 |
| clonal occurrences of multidrug-resistant gram-negative bacilli: report from the meropenem yearly susceptibility test information collection surveillance program in the united states (2004). | the meropenem yearly susceptibility test information collection program is a global, longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. between 1999 and 2004, a total of 10-16 us medical centers referred up to 200 nonduplicate isolates from clinical infections to a central processing laboratory. over this 6-year period, the antimicrobial activity of 12 broad-s ... | 2006 | 16466890 |
| particularities of bacterial ecology of acute otitis media in an african subtropical country (cote d'ivoire). | acute otitis media (aom) is the commonest infectious disease of childhood. its treatment is based on a probabilistic antibiotic therapy. | 2006 | 16297453 |
| tryptophanase from proteus vulgaris: the conformational rearrangement in the active site, induced by the mutation of tyrosine 72 to phenylalanine, and its mechanistic consequences. | tyr72 is located at the active site of tryptophanase (trpase) from proteus vulgaris. for the wild-type trpase tyr72 might be considered as the general acid catalyst at the stage of elimination of the leaving groups. the replacement of tyr72 by phe leads to a decrease in activity for l-tryptophan by 50,000-fold and to a considerable rearrangement of the active site of trpase. this rearrangement leads to an increase of room around the alpha-c atom of any bound amino acid, such that covalent bindin ... | 2006 | 16455316 |
| isolation of salmonella bacteriophages from swine effluent lagoons. | bacteriophages (phages) associated with salmonella were collected from nine swine manure lagoons in mississippi. phages were isolated by an enrichment protocol or directly from effluent. for enrichment, chloroform-treated samples were filtered (0.22 mum) and selectively enriched by adding a cocktail of salmonella strains in trypticase soy broth. after overnight incubation at 35 degrees c, chloroform was added and samples stored at 5 degrees c. enriched samples were tested by double agar layer (d ... | 2006 | 16455853 |
| mössbauer identification of a protonated ferryl species in catalase from proteus mirabilis: density functional calculations on related models. | the proteus mirabilis catalase is one of the most efficient heme-containing catalase and forms a relatively stable compound ii. samples of compound ii were prepared from pmc enriched in (57)fe. for the first time, two different forms of compound ii, namely low ph compound ii (lph ii) (43%) and high ph compound ii (hph ii) (25%), have been characterized by mössbauer spectroscopy at ph 8.3. the ratio lph ii/hph ii increases irreversibly with decreasing ph. the large quadrupole splitting value of l ... | 2006 | 16442627 |
| a new gastrointestinal finding in proteus syndrome: report of a case of multiple colonic hemangiomas. | a patient with proteus syndrome presented with lower gastrointestinal bleeding due to multiple colonic hemangiomas, a finding which has not been described previously in this syndrome. the patient was a 20-year-old man with features fulfilling the diagnostic criteria for proteus syndrome. he fulfilled both general criteria (mosaic distribution of the lesions, progressive course and sporadic occurrence) and specific criteria (including epidermal nevus, disproportionate overgrowth of limbs and vasc ... | 2006 | 16445505 |
| cmy-16, a novel acquired ampc-type beta-lactamase of the cmy/lat lineage in multifocal monophyletic isolates of proteus mirabilis from northern italy. | we report multifocal detection (four different cities in northern italy) of proteus mirabilis isolates resistant to both oxyimino- and 7-alpha-methoxy-cephalosporins and producing a novel acquired ampc-like beta-lactamase. the enzyme, named cmy-16, is a variant of the cmy/lat lineage, which differs from the closest homologues, cmy-4 and cmy-12, by a single amino acid substitution (a171s or n363s, respectively) and from cmy-2 by two substitutions (a171s and w221r). expression of the cloned bla(cm ... | 2006 | 16436718 |
| prospective study of individuals with long-term urinary catheters colonized with proteus species. | to characterize the variability in the times catheters take to block with encrustation in patients who have proteus in their urinary flora, and to identify factors responsible for modulating the rate of catheter encrustation and blockage. | 2006 | 16336341 |
| characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1. | to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples. | 2006 | 16430517 |
| rigid sigmoidoscopy: a potential hazard for cross-contamination. | rigid sigmoidoscopy using a disposable or nondisposable sigmoidoscope is a common outpatient procedure. it has been assumed that the nondisposable bellows and light head of the sigmoidoscope remain free from enteric organisms so that the procedure is sterile if a disposable or nondisposable (metal) sigmoidoscope shaft is used. the aim of this study was to identify the presence of organisms within the bellows or light head of the sigmoidoscope. | 2006 | 16418794 |
| perioperative respiratory complications caused by cystic lung malformation in proteus syndrome. | a 7-year-old girl with proteus syndrome, complicated with severe kyphoscoliosis and cystic lung malformation, developed respiratory complications during and after posterior spinal fusion under general anesthesia. we speculated that low oxygenation while in the prone position contributed to ventilation perfusion mismatch owing to the cystic lung malformation that existed predominantly in the right lung, and that postoperative respiratory failure was caused by initially increased bronchial mucous ... | 2006 | 16421672 |
| evaluation of a novel kit for the rapid detection of extended-spectrum beta-lactamases. | since a method of rapidly detecting extended-spectrum beta-lactamase (esbl) production in gram-negative isolates from patients with severe infection is urgently required, the present study of a novel commercial kit was conducted. the cica-beta test i (kanto chemical, tokyo, japan) is designed for the rapid detection of esbl in gram-negative bacteria directly from isolated colonies in a 15-min protocol. in this study, a total of 304 strains of klebsiella spp., escherichia coli and proteus mirabil ... | 2006 | 16416268 |
| comparative analysis of a modified ecolite method, the colicomplete method, and a most-probable-number method for detecting escherichia coli in orange juice. | the ecolite high volume juice (hvj) presence-absence method for a 10-ml juice sample was compared with the u.s. food and drug administration bacteriological analytical manual most-probable-number (mpn) method for analysis of artificially contaminated orange juices. samples were added to ecolite-hvj medium and incubated at 35 degrees c for 24 to 48 h. fluorescent blue results were positive for glucuronidase- and galactosidase-producing microorganisms, specifically indicative of about 94% of esche ... | 2006 | 16416921 |
| occurrence of extended-spectrum (beta)-lactamases and plasmid-mediated ampc (beta)-lactamases among korean isolates of proteus mirabilis. | 2006 | 16284101 | |
| synthesis and evaluation of new quinazolone derivatives of nalidixic acid as potential antibacterial and antifungal agents. | in continuation of our work on synthesis of biheterocycles carrying the biodynamic heterocyclic systems at position 3, a series of new nalidixic acid derivatives having quinazolones moiety were synthesised to achieve enhanced biological activity and wide spectrum of activity. nalidixic acid was first converted into its acid chloride using thionyl chloride as an acylating agent at laboratory temperature. later it was converted to methyl ester. nalidixoyl chloride formed vigorously reacts with met ... | 2006 | 16260068 |
| evolutionarily conserved structural motifs in bacterial gst (glutathione s-transferase) are involved in protein folding and stability. | the bacterium proteus mirabilis expresses a cytosolic class beta glutathione s-transferase (pmgst b1-1) that is part of a family of multifunctional detoxication enzymes. like other cytosolic gsts, pmgst b1-1 possesses two local structural motifs, an n-capping box and a hydrophobic staple motif, both of which are located between amino acids 151 and 156. the n-capping box consists of a reciprocal hydrogen bonding interaction of thr152 with asp155, whereas the hydrophobic staple motif consists of a ... | 2006 | 16248855 |
| evaluation of antimicrobial activity of cleome viscosa and gmelina asiatica. | the ethanolic extracts of the leaves and flowers of cleome viscosa and roots of gmelina asiatica were tested for antimicrobial activity. the two plants exhibited a broad spectrum of antimicrobial activity, particularly significative against escherichia coli , proteus vulgaris and pseudomonas aeruginosa. the leaf extract of c. viscosa showed moderate activity against pathogenic fungi. | 2006 | 16325351 |
| a herd level analysis of urinary tract infection in dairy cattle. | cystitis, urethritis and pyelonephritis in cattle most commonly result from ascending urinary tract infection with corynebacterium renale, corynebacterium cystidis, corynebacterium pilosum or escherichia coli. we describe the clinical, bacteriological, clinical-pathological and epidemiological findings in a dairy cattle herd with urinary tract infection (uti). blood and urine samples from 17 calves and 19 cows were submitted to laboratory examinations. depression, muscle wasting, weakness and fr ... | 2006 | 16310382 |
| dna sequence analysis of the genetic environment of various blactx-m genes. | over a 3 year period (2000-2003) 21 escherichia coli, 5 klebsiella pneumoniae, 1 serratia marcescens and 1 proteus mirabilis producing ctx-m-type beta-lactamase were collected from five different hospitals in paris, france. this study was conducted to analyse the genetic environment of these 28 bla(ctx-m) genes. | 2006 | 16291869 |
| bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). | wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ... | 2006 | 16382282 |
| antimicrobial activities of silver dressings: an in vitro comparison. | a range of silver-coated or -impregnated dressings are now commercially available for use but comparative data on their antimicrobial efficacies are limited. the antibacterial activities of five commercially available silver-coated/impregnated dressings were compared against nine common burn-wound pathogens, namely methicillin-sensitive and -resistant staphylococcus aureus (mrsa), enterococcus faecalis, pseudomonas aeruginosa, escherichia coli, enterobacter cloacae, proteus vulgaris, acinetobact ... | 2006 | 16388031 |
| the incidence of proteus mirabilis infection increases in patients on treatment but does not trigger disease activity. | the aim of this study is to determine whether treatment increases the levels of anti-proteus antibodies (apa) in patients with rheumatoid arthritis (ra). the blood samples of 32 patients suffering from ra who were recruited in our previous study and continued to participate in our follow-up study were collected after 1 year. their first and follow-up samples were analysed for the presence of igg isotype and total immunoglobulins (igg+iga+igm) against proteus mirabalis (pm) using enzyme-linked im ... | 2006 | 16267598 |
| dissemination of proteus mirabilis isolates harboring ctx-m-14 and ctx-m-3 beta-lactamases at 2 hospitals in taiwan. | from february to june 2003, 111 clinical isolates of proteus mirabilis were mainly isolated from patients with respiratory or urinary tract infections hospitalized at 3 district hospitals (a, b, c) in central taiwan. among them, 34 (30.6%) strains, isolated within 2 hospitals (a and b), exhibited nonsusceptibility to cefotaxime with significant reduction of mic (> or = 3 log2 dilution) by the effect of clavulanic acid, which confirmed the phenotype of extended-spectrum beta-lactamases (esbls). t ... | 2006 | 16406185 |
| a structured-population model of proteus mirabilis swarm-colony development. | in this paper we present continuous age- and space-structured models and numerical computations of proteus mirabilis swarm-colony development. we base the mathematical representation of the cell-cycle dynamics of proteus mirabilis on those developed by esipov and shapiro, which are the best understood aspects of the system, and we make minimum assumptions about less-understood mechanisms, such as precise forms of the spatial diffusion. the models in this paper have explicit age-structure and, wh ... | 2006 | 16283413 |
| effect of triclosan on the development of bacterial biofilms by urinary tract pathogens on urinary catheters. | to examine (i) the effect of triclosan on the formation of catheter biofilms by urinary tract pathogens and (ii) the diffusion of triclosan through the retention balloons of urinary catheters. | 2006 | 16339844 |
| colonization of sitka spruce stumps by decay-causing hymenomycetes in paired inoculations. | sitka spruce stumps were inoculated with decay fungi using colonized sawdust or dowel inoculum to investigate colonization in paired combinations. estimates of domain sizes were made in the top 15cm of stump after 13-14 or 21-23 months with sawdust or dowel inoculations, respectively. none of the co-inoculated species prevented colonization by heterobasidion annosum; sapwood colonization by resinicium bicolor may limit growth of h. annosum colonies out of heartwood, reducing the incidence of dis ... | 2006 | 16814536 |
| characterisation of prototype nurmi cultures using culture-based microbiological techniques and pcr-dgge. | undefined nurmi-type cultures (ntcs) have been used successfully to prevent salmonella colonisation in poultry for decades. such cultures are derived from the caecal contents of specific-pathogen-free birds and are administered via drinking water or spray application onto eggs in the hatchery. these cultures consist of many non-culturable and obligately anaerobic bacteria. due to their undefined nature it is difficult to obtain approval from regulatory agencies to use these preparations as direc ... | 2006 | 16814892 |
| [proteus syndrome with cerebral vascular malformations]. | proteus syndrome is a congenital hamartomatous dysplasia. this sporadic disorder involves the skeletal system, soft tissues, skin and vascular system. the most likely pathogenesis involves somatic mosaicism. main manifestations included soft-tissue and epidermal nevi, partial gigantism, hemihypertrophy, exostoses, lipomas and vascular anomalies. the most common brain abnormalities are hemimegencephaly and migrational disorders. we present a case of proteus syndrome with cerebral vascular anomali ... | 2006 | 16525914 |
| [protective effect of the aerosol immunization of mice with the polycomponent vaccine immunovac vp-4 after the challenge of the animals with klebsiella pneumoniae virulent strain]. | used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with k. pneumoniae virulent strain k16. irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized ani ... | 2006 | 16532635 |
| biofilm formation in an ice cream plant. | the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ... | 2006 | 16779628 |
| structure and evolution of the mitochondrial control region and flanking sequences in the european cave salamander proteus anguinus. | the european cave salamander proteus anguinus laurenti 1768 is one of the best-known subterranean animals, yet its evolutionary history and systematic relationships remain enigmatic. this is the first comprehensive study on molecular evolution within the taxon, using an mtdna segment containing the control region (cr) and adjacent sequences. two to seven tandem repeats of 24-32 bp were found in the intergenic spacer region (vntr1), and three, four or six repeats, 59-77 bp each, in the 3' end of ... | 2006 | 16764998 |
| proteus mirabilis spondylodiscitis complicating a urinary tract infection. | infectious spondylodiscitis results from local or haematogenous infection by microorganisms of intervertebral disc spaces, vertebral bodies and surrounding structures. haematogenous invasion may follow urosepsis. we report on a case of septic spondylodiscitis following urosepsis with proteus mirabilis, a frequently isolated microorganism in urinary tract infections but rarely in spondylodiscitis. | 2006 | 16798719 |
| rapid onset of ulcerative typhlocolitis in b6.129p2-il10tm1cgn (il-10-/-) mice infected with helicobacter trogontum is associated with decreased colonization by altered schaedler's flora. | infection with helicobacter trogontum, a urease-positive helicobacter isolated from subclinically infected rats, was evaluated in b6.129p2-il10(tm1cgn) (interleukin-10(-/-) [il-10(-/-)]) and c57bl/6 (b6) mice. in a first experiment, il-10(-/-) mice naturally infected with helicobacter rodentium had subclinical typhlocolitis but developed severe diarrhea and loss of body condition with erosive to ulcerative typhlocolitis within 1 to 3 weeks of experimental infection with h. trogontum. a second ex ... | 2006 | 16982822 |
| multiple bacterial species reside in chronic wounds: a longitudinal study. | the aim of the study was to investigate the bacterial profile of chronic venous leg ulcers and the importance of the profile to ulcer development. patients with persisting venous leg ulcers were included and followed for 8 weeks. every second week, ulcer samples were collected and the bacterial species present were identified. more than one bacterial species were detected in all the ulcers. the most common bacteria found were staphylococcus aureus (found in 93.5% of the ulcers), enterococcus fae ... | 2006 | 16984578 |
| [development of a gold-immunochromatography test for rapid detecting e. coli o157]. | to develop a method for rapid detecting escherichia coli (e. coli) o157 on site. | 2006 | 16986519 |
| antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents. | the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ... | 2006 | 16989308 |
| proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice. | proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (utis). in this study, a collection of p. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. isolates were characterized in vitro, assessing several virulence properties that had been previously associated with p. mirabi ... | 2006 | 16804188 |
| phytochemical and antimicrobial study of an antidiabetic plant: scoparia dulcis l. | the antimicrobial and antifungal effects of different concentrations of chloroform/methanol fractions of scoparia dulcis were investigated. the isolated fractions were tested against different bacteria like salmonella typhii, staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa, and proteus vulgaris and fungal strains such as alternaria macrospora, candida albicans, aspergillus niger, and fusarium oxysporum. the isolated fractions exhibited significant antimicrobial ... | 2006 | 17004904 |
| mapping bacterial surface population physiology in real-time: infrared spectroscopy of proteus mirabilis swarm colonies. | we mapped the space-time distribution of stationary and swarmer cells within a growing proteus mirabilis colony by infrared (ir) microspectroscopy. colony mapping was performed at different positions between the inoculum and the periphery with a discrete microscope-mounted ir sensor, while continuous monitoring at a fixed location over time used an optical fiber based ir-attenuated total reflection (atr) sensor, or "optrode." phenotypes within a single p. mirabilis population relied on identific ... | 2006 | 16808858 |
| in vitro activities of three carbapenems against recent bacterial isolates from severely ill patients at swedish hospitals. | to study the in vitro activity of imipenem, meropenem and ertapenem against common pathogens isolated from patients in intensive care, haematology and dialysis/nephrology units at 7 swedish university hospitals, a total of 788 isolates were collected during 2002-2003. the distribution of the isolates was as follows: escherichia coli (n = 140), klebsiella spp. (n = 132), proteus spp. (n = 97), enterobacter spp. (n = 113), pseudomonas aeruginosa (n = 126), acinetobacter spp. (n = 53) and enterococ ... | 2006 | 17008228 |
| urinary bactericidal activity of oral antibiotics against common urinary tract pathogens in an ex vivo model. | in this investigation, the urine samples obtained in a single oral-dose pharmacokinetic study were examined for their bactericidal activity against a range of relevant urinary tract pathogens. | 2006 | 17008781 |
| etiologic agents of cervicovaginitis in turkish women. | to investigate the distribution of microbiologic agents causing cervicovaginitis. | 2006 | 17013472 |
| pharmacokinetic and pharmacodynamic aspects of antimicrobial agents for the treatment of uncomplicated urinary tract infections. | uncomplicated urinary tract infections (uti) are treated with beta-lactams, co-trimoxazole, quinolones and fosfomycin tromethamine. due to increasing resistance of causative pathogens, antibiotics should be used by considering their pharmacodynamic and pharmacokinetic characteristics. beta-lactams have time-dependent activity and should not be used once-daily. co-trimoxazole should be restricted due to increasing chemoresistance. fluoroquinolones play a primary role in the treatment of serious a ... | 2006 | 16829051 |
| [antimicrobial susceptibility of proteus mirabilis urinary tract isolates from 1999 to 2005 at nîmes university hospital]. | to analyse antimicrobial resistance trends of proteus mirabilis urinary tract isolates from 1999 to 2005 at the nîmes university hospital. | 2006 | 17030456 |
| hospital acquired urinary tract infections in urology departments: pathogens, susceptibility and use of antibiotics. data from the pep and peap-studies. | data from two internet-based studies on nauti in hospitalized urological patients are presented together: the pan european prevalence (pep) study, which was a 1-day prevalence study in november 2003; and the pan euro-asian prevalence (peap) study, which was carried out in november 2004. overall, 93 and 101 hospitals from the two studies, respectively, completed the hospital questionnaires and provided patient information for the present study. nauti was diagnosed according to the centres for dis ... | 2006 | 16829052 |
| proteus syndrome with agenesis of the rectus abdominis. | 2006 | 17034561 | |
| [rapid detection of pseudomonas aeruginosa by the fluorescence quantitative pcr assay targeting 16s rdna]. | the 16s rdna specific primers were designed for rapid detection of pseudomonas aeruginosa (pa) by the fluorescence quantitative pcr (fq-pcr) assay, based upon multiple sequence alignment and phylogenetic tree analysis of the 16s rdnas of over 20 bacteria. after extraction of pa genomic dna, the target 16s rdna fragment was amplified by pcr with specific primers, and used to construct recombinant pmdt-pfr plasmid, the dilution gradients of which were subjected to the standard quantitation curve i ... | 2006 | 17037203 |
| performance in detection and reporting beta-lactam resistance phenotypes in enterobacteriaceae: a nationwide proficiency study in italian laboratories. | we evaluated the ability of 60 italian clinical microbiology laboratories in detecting and reporting beta-lactam resistance phenotypes in enterobacteriaceae. laboratories received 5 well-characterized isolates producing extended-spectrum beta-lactamases (esbls), 2 hyperproducers of chromosomal enzymes, and 3 quality control strains. the performances in antimicrobial susceptibility testing (ast) were different depending on the species and type of esbl produced. high rates of very major errors (up ... | 2006 | 16678993 |
| antimicrobial activity of some ethnomedicinal plants used by paliyar tribe from tamil nadu, india. | antimicrobial activity of 18 ethnomedicinal plant extracts were evaluated against nine bacterial strains (bacillus subtilis, staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa, ervinia sp, proteus vulgaris) and one fungal strain (candida albicans). the collected ethnomedicinal plants were used in folk medicine in the treatment of skin diseases, venereal diseases, respiratory problems and nervous disorders. | 2006 | 17042964 |
| bactericidal activity of ertapenem against major intra-abdominal pathogens. | treatment of intra-abdominal infections remains a challenge owing to their polymicrobial nature and associated mortality risk. treatment regimens must provide broad-spectrum coverage, including gram-positive and gram-negative aerobic and anaerobic bacteria of gastrointestinal origin. ertapenem is a long-acting 1-beta-methyl parenteral group 1 carbapenem antibiotic that has a broad antibacterial spectrum and once-daily dosing supported by clinical studies. it is active against gram-positive and g ... | 2006 | 17045463 |
| [characteristics of war wound infection]. | war wounds are the most complex type of non-targeted injuries due to uncontrolled tissue damage of varied and multifold localizations, exposing sterile body areas to contamination with a huge amount of bacteria. wound contamination is caused by both the host microflora and exogenous agents from the environment (bullets, cloth fragments, dust, dirt, water) due to destruction of the host protective barriers. war wounds are the consequence of destructive effects of various types of projectiles, whi ... | 2006 | 17048790 |
| [development of beta-lactamase resistance in enterobacteria]. | enterobacteria produce elementary chromosomal enzymes, beta-lactamases of class a: tem and shv (escherichia coli, klebsiella pneumoniae). these can give rise to plasmid-coded broad-spectrum beta-lactamases (esbl) discovered in 1980 (e. coli, k. pneumoniae, enterobacter cloacae). the first cefotaximase (ctx-m, men-1) was reported in europe in 1990. this enzyme is far more active against cefotaxime than against ceftazidime and aztreonam. chromosomal hyperpoduction of k1 beta-lactamase differs from ... | 2006 | 17051471 |
| human gamma delta t cells and tumor immunotherapy. | human vgamma2vdelta2 t cells recognize nonpeptide antigens derived from pathogenic microbes in a tcr-dependent manner, such as pyrophosphomonoester compounds from mycobacteria and malaria parasite and alkyl amines from proteus, suggesting that this subset of gamma delta t cells is involved in infectious immunity. the precise recognition mechanism has been delineated using a site-directed mutagenesis strategy based on crystal structure of gamma delta tcr. on the other hand, several lines of evide ... | 2006 | 17058804 |
| metalloantibiotics: synthesis, characterization and in-vitro antibacterial studies on cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes with cloxacillin. | the synthesis and characterization of cloxacillin (clox) complexes with divalent metal ions [co (ii), cu (ii), ni (ii) and zn (ii)] is described. the nature of bonding of the chelated cloxacillin and the structures of the metal complexes have been elucidated on the basis of their physical and spectroscopic data. in all the complexes, the cloxacillin acts as a uninegatively charged bidentate ligand with coordination involving the carboxylate-o and endocyclic-n of the beta-lactam ring. the new com ... | 2006 | 17059178 |
| [heat shock proteins of freshwater protists and their involvement in adaptation to changes in the environmental salinity]. | changes in the level of heat shock proteins (hsp) in cells of freshwater protists, amoebae amoeba proteus and ciliates paramecium jenningsi, in response to changes in the environmental salinity were investigated. changes in salinity levels were considered as a stress factor. the immunoblotting method revealed a polypeptide antigen cross-reacting with antibodies against bovine hsp70 in total protein extracts of both intact cells and cells subjected to salinity stress. the same polypeptide antigen ... | 2006 | 16893060 |
| the challenges of proteus syndrome: diagnosis and management. | proteus syndrome (ps) is a disorder of patchy or mosaic postnatal overgrowth of unknown etiology. the onset of overgrowth typically occurs in infancy and can involve any tissue of the body. commonly involved tissues include connective tissue and bone, skin, central nervous system, the eye, but it apparently can affect any tissue. diagnosing of ps is difficult and the diagnostic criteria are controversial. our group advocates stringent diagnostic criteria to facilitate research and appropriate cl ... | 2006 | 16883308 |
| [a young patient with portal and mesenteric vein thrombosis and proteus syndrome]. | in the 26th year of life a young woman suffered a portal and mesenteric thrombosis followed by portal hypertension with splenomegaly, esophageal varices and pancytopenia. after splenorenal shunt surgery and splenectomy hematologic parameters resolved rapidly. also, she was suffering of proteus syndrome, which is an extremely rare and sporadic hamartomatous disorder characterized by a variety of cutaneous and subcutaneous tumors including vascular malformations, several types of nevi, partial gig ... | 2006 | 16758842 |
| [extended-spectrum-beta-lactamase-producing proteus mirabilis: laboratory-based surveillance in cooperation with 12 clinical laboratories in the kinki region of japan]. | we studied 247 strains of proteus mirabilis collected during the 6 months from november 2003 to april 2004 from 12 clinical laboratories in the kinki region of japan for the production of extended-spectrum beta-lactamase (esbl). eighteen strains (7.3%) showed mics for cefpodoxime of > or = 2 microg/ml and 13 strains (5.2%) were positive for the double-disk synergy test. susceptibility depended on genotype. mics for cefepime, cefozopran, and cefpirome were high (> or = 8 microg/ml), and that for ... | 2006 | 16780129 |
| doppler flow imaging of cytoplasmic streaming using spectral domain phase microscopy. | spectral domain phase microscopy (sdpm) is a function extension of spectral domain optical coherence tomography. sdpm achieves exquisite levels of phase stability by employing common-path interferometry. we discuss the theory and limitations of doppler flow imaging using sdpm, demonstrate monitoring the thermal contraction of a glass sample with nanometer per second velocity sensitivity, and apply this technique to measurement of cytoplasmic streaming in an amoeba proteus pseudopod. we observe r ... | 2006 | 16674204 |
| surgical site infections in day surgery settings. | because major complications are rare in day surgery, minor morbidities such as surgical site infections, which become evident only after patient discharge, have a major impact on the perceived quality of care. we performed an audit of such infections over a oneyear period. | 2006 | 16895493 |
| new trends in prosthesis infection in cardiovascular surgery. | adult cardiovascular surgery entails, in many cases, the use of some kind of prosthesis. among the potential complications, prosthetic device infection is one of the most devastating in incidence, as well as in prognosis and damage to surrounding tissues. | 2006 | 16895504 |
| emergence of proteus mirabilis carrying the bla metallo-beta-lactamase gene. | seven genetically related proteus mirabilis clinical isolates from a hospital in thessaloniki, greece, exhibited decreased susceptibility to imipenem and carried a bla(vim-1) metallo-beta-lactamase gene. pcr mapping revealed that bla(vim-1) was part of a class 1 integron that was probably located in the chromosome and also included the aaca7, dhfr and aada genes. this is the first description of the bla(vim-1) metallo-beta-lactamase gene in p. mirabilis. | 2006 | 16774572 |
| improving the accuracy of protein secondary structure prediction using structural alignment. | the accuracy of protein secondary structure prediction has steadily improved over the past 30 years. now many secondary structure prediction methods routinely achieve an accuracy (q3) of about 75%. we believe this accuracy could be further improved by including structure (as opposed to sequence) database comparisons as part of the prediction process. indeed, given the large size of the protein data bank (>35,000 sequences), the probability of a newly identified sequence having a structural homol ... | 2006 | 16774686 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2004). i. susceptibility distribution]. | the bacterial strains isolated from 490 patients diagnosed as having urinary tract infections (utis) in 14 institutions in japan were collected between august 2004 and july 2005. the susceptibilities of them to many kinds of antimicrobial agents were measured. of them, 577 strains were estimated as causative bacteria and used for the measurement. the strains consisted of 156 gram-positive bacterial strains (27.0%) and 421 gram-negative bacterial strains (73.0%). against staphylococcus aureus, ar ... | 2006 | 16913404 |
| how long do nosocomial pathogens persist on inanimate surfaces? a systematic review. | inanimate surfaces have often been described as the source for outbreaks of nosocomial infections. the aim of this review is to summarize data on the persistence of different nosocomial pathogens on inanimate surfaces. | 2006 | 16914034 |
| pcr for the identification and differentiation of histomonas meleagridis, tetratrichomonas gallinarum and blastocystis spp. | in the present investigation pcr assays were developed for the rapid detection and differentiation of two poultry flagellates: histomonas meleagridis and tetratrichomonas gallinarum as well as the protozoan microorganism: blastocystis spp. the nucleotide sequences of the small subunit ribosomal rnas were used for primer construction obtaining fragments which vary in size for each microorganism. the established pcrs were able to detect dna obtained from one microorganism of t. gallinarum and blas ... | 2006 | 16920265 |
| a comparison of antimicrobial susceptibility profile of urinary pathogens for the years, 1999 and 2003. | urinary tract infection is a common condition worldwide; responsible for significant morbidity in both hospitalized and community patients. the laboratory records, for microbial isolates of infected urine and their susceptibility profiles for the years 1999 and 2003 were retrospectively reviewed and compared. in 2003, there was a significant decline in recovery ofcitrobacter spp compared to 1999. conversely, the proportion of k pneumoniae, e coli and enterococci increased dramatically in 2003, i ... | 2006 | 16921702 |
| fishmeal extract bile salt lactose agar--a differential medium for enteric bacteria. | fishmeal extract bile salt lactose agar (febla), a new differential medium for enteric bacteria was developed and evaluated for its ability to grow and differentiate lactose fermenters (lf) from non-lactose fermenters (nlf) in comparison with macconkeys agar. performance of febla was at par with the latter. on febla medium, the contrast between lf and nlf colonies was pronounced and klebsiella pneumoniae produced more mucoid colonies than on macconkeys agar (hi media). unlike macconkeys agar, a ... | 2006 | 16924840 |
| emerging resistance to carbapenems in a tertiary care hospital in north india. | carbapenems are beta-lactam antibiotics, presently considered as most potent agents for treating multi-drug resistant gram-negative bacilli infections. in india carbapenems available for use are meropenem and imipenem, introduced recently. resistance to these has been reported in a few bacteria especially pseudomonas spp. we therefore retrospectively evaluated the antibiotic susceptibility pattern to these agents amongst various clinical isolates in a tertiary care hospital in north india. | 2006 | 16926463 |
| extended-spectrum beta-lactamases in taiwan: epidemiology, detection, treatment and infection control. | extended-spectrum beta-lactamases (esbls) efficiently hydrolyze extended-spectrum beta-lactams such as cefotaxime, ceftriaxone, ceftazidime, and aztreonam. esbls are most often plasmid-mediated. in taiwan, the prevalence of esbls in bacteria has risen, ranging from 8.5 to 29.8% in klebsiella pneumoniae and 1.5 to 16.7% in escherichia coli isolates. the most prevalent types of esbls are shv-5, shv-12, ctx-m-3, and ctx-m-14 in isolates of k. pneumoniae and e. coli, with differences between institu ... | 2006 | 16926972 |
| 1h nmr spectroscopy in the diagnosis of klebsiella pneumoniae-induced urinary tract infection. | the (1)h nmr spectroscopic method is suggested and its utility is demonstrated for the diagnosis of klebsiella pneumoniae (k. pneumoniae) in urinary tract infection (uti). k. pneumoniae have the specific property of metabolizing glycerol to 1,3-propanediol (1,3-pd), acetate, ethanol and succinate. the quantity of 1,3-pd produced correlates well with the viable bacterial count. other common bacteria causing uti (except for citrobacter frundii), such as escherichia coli (e. coli), pseudomonas aeru ... | 2006 | 16927393 |
| comparison of susceptibility to antimicrobials of bacterial isolates from companion animals in a veterinary diagnostic laboratory in canada between 2 time points 10 years apart. | the susceptibility to antimicrobials of bacterial species most frequently isolated from companion animals in a veterinary teaching diagnostic laboratory was evaluated retrospectively. a significant decrease between 1990-1992 and 2002-2003 was noted in the susceptibility of dog isolates to the following antimicrobials: escherichia coli to cephalothin (86% to 61%, p < 0.001); e. coli to ampicillin (85% to 67%, p < 0.001); proteus spp. to ampicillin (92% to 71%, p < 0.01); coagulase-positive staphy ... | 2006 | 16933555 |
| assessment of pathogen frequency and resistance patterns among pediatric patient isolates: report from the 2004 sentry antimicrobial surveillance program on 3 continents. | selecting empiric or directed therapy for pathogens isolated from pediatric patients can be problematic. many antimicrobial agents are not indicated for use in pediatric patients, and regional variations of resistance mechanisms have been reported. the purpose of this study was to analyze antimicrobial resistance patterns and pathogen occurrence rates in pediatric-aged patient infections on 3 continents using data from the sentry antimicrobial surveillance program. a total of 3537 clinical isola ... | 2006 | 16938419 |
| effective prevention of microbial biofilm formation on medical devices by low-energy surface acoustic waves. | low-energy surface acoustic waves generated from electrically activated piezo elements are shown to effectively prevent microbial biofilm formation on indwelling medical devices. the development of biofilms by four different bacteria and candida species is prevented when such elastic waves with amplitudes in the nanometer range are applied. acoustic-wave-activated foley catheters have all their surfaces vibrating with longitudinal and transversal dispersion vectors homogeneously surrounding the ... | 2006 | 16940055 |
| mode of transposition and expression of 16s rrna methyltransferase gene rmtc accompanied by isecp1. | a newly identified 16s rrna methyltransferase gene, rmtc, was accompanied by an isecp1 element at its 5' end. this isecp1 element, which contained a transposase gene, tnpa, provided a promoter activity for expression of the adjacent rmtc; and this structure enabled the rmtc gene to be transposed onto another plasmid in escherichia coli. | 2006 | 16940134 |
| relevance of weil-felix test in diagnosis of scrub typhus in india. | to study the relationship of weil-felix test and microimmunofluorescence test. | 2006 | 16941792 |
| triclosan loaded ureteral stents decrease proteus mirabilis 296 infection in a rabbit urinary tract infection model. | infection and encrustation remain major limitations of ureteral stent use and to our knowledge no device has completely overcome these obstacles to date. triclosan is a biocide currently used in a plethora of consumer and medical products that has recently been loaded into a ureteral stent. using a rabbit model of uti we examined the effects of triclosan impregnated stent segments on the growth and survival of proteus mirabilis, a uropathogen commonly associated with device related uti and encru ... | 2006 | 16697868 |
| structure of the glycerol phosphate-containing o-polysaccharides and serological studies of the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 classified into a new proteus serogroup, o54. | o-polysaccharides were obtained from the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 and studied by chemical analyses and one- and two-dimensional (1)h and (13)c nuclear magnetic resonance spectroscopy, including rotating-frame nuclear overhauser effect spectroscopy, h-detected (1)h,(13)c heteronuclear single-quantum spectroscopy and (1)h,(31)p heteronuclear multiple-quantum spectroscopy experiments. the proteus mirabilis oe polysaccharide was found to ha ... | 2006 | 16831214 |
| the dps gene of symbiotic "candidatus legionella jeonii" in amoeba proteus responds to hydrogen peroxide and phagocytosis. | to survive in host cells, intracellular pathogens or symbiotic bacteria require protective mechanisms to overcome the oxidative stress generated by phagocytic activities of the host. by genomic library tagging, we cloned a dps (stands for dna-binding protein from starved cells) gene of the symbiotic "candidatus legionella jeonii" organism (called the x bacterium) (dps(x)) that grows in amoeba proteus. the gene encodes a 17-kda protein (pi 5.19) with 91% homology to dps and dna-binding ferritin-l ... | 2006 | 16950918 |
| the comparison of pyrosequencing molecular gram stain, culture, and conventional gram stain for diagnosing orthopaedic infections. | we have developed a combined real-time pcr and pyrosequencing assay that successfully differentiated the vast majority of gram-positive and gram-negative bacteria when bacterial isolates were tested. the purpose of this study was to evaluate this assay on clinical specimens obtained from orthopedic surgeries, and to prospectively compare the results of "molecular gram stain" with culture and conventional direct gram stain. forty-five surgical specimens were obtained from patients who underwent o ... | 2006 | 16788984 |
| analysis on antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital. | the distinction of antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital was investigated. disc diffusion test was used to study the antimicrobial resistance of isolates collected from county hospitals and a teaching hospital. the data was analyzed by whonet5 and spss statistic software. a total of 655 strains and 1682 strains were collected from county hospitals and a teaching hospital, respectively, in the year of 2003. the top ten pathogens were ... | 2006 | 16961303 |
| occurrence of qnra-positive clinical isolates in french teaching hospitals during 2002-2005. | bacteria harbouring the novel qnra plasmid-mediated mechanism of quinolone resistance have been described in different countries, but the frequency of their occurrence has not been investigated. in total, 1,468 clinical isolates of enterobacteriaceae with quinolone resistance or extended-spectrum beta-lactamase (esbl) phenotypes were collected from eight teaching hospitals in france during 2002-2005 and screened for qnra. overall, 28 isolates (22 enterobacter cloacae, three klebsiella pneumoniae ... | 2006 | 16961639 |
| the catalytic machinery of chondroitinase abc i utilizes a calcium coordination strategy to optimally process dermatan sulfate. | the chondroitinases are bacterial lyases that specifically cleave chondroitin sulfate and/or dermatan sulfate glycosaminoglycans. one of these enzymes, chondroitinase abc i from proteus vulgaris, has the broadest substrate specificity and has been widely used to depolymerize these glycosaminoglycans. biochemical and structural studies to investigate the active site of chondroitinase abc i have provided important insights into the catalytic amino acids. in this study, we demonstrate that calcium, ... | 2006 | 16964974 |
| synthesis, structure and biological activity of cobalt(ii) and copper(ii) complexes of valine-derived schiff bases. | we have synthesized two cobalt(ii) 2 and copper(ii) 3 complexes of valine-derived schiff bases. the obtained complexes were characterized by elemental analysis, ft-ir and x-ray diffraction. biological studies of complexes 2 and 3 had been carried out in vitro for antimicrobial activity against gram-positive, gram-negative bacteria and human pathogenic fungi. compound 3 was proven to be a broad spectrum agent, showed a significant inhibition of the growth of gram-positive bacteria (staphylococcus ... | 2006 | 16965817 |
| ft-ir microspectroscopy for early identification of some clinically relevant pathogens. | to investigate the potentials and limitations of fourier transform-infrared (ft-ir) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment. | 2006 | 16968290 |
| nutritive value, antioxidant and antimicrobial properties of struchium sparganophora leaves. | struchium sparganophora leaf is a green leafy vegetable popularly used in soup preparations in processed and unprocessed forms in nigeria. fresh leaves of s. sparganophora were soaked overnight to remove its bitterness, before determination of the nutrient and antinutrient content, while sun-dried s. sparganophora leaves were extracted with rectified spirit. the antioxidant and antimicrobial properties of the extracts were subsequently determined. the result of the study revealed that the fresh ... | 2006 | 16822216 |
| [a 56-year-old woman with pain and crepitations in the left lower limb]. | 2006 | 16606564 | |
| comparison of fecal microflora in children with atopic eczema/dermatitis syndrome according to ige sensitization to food. | atopic eczema/dermatitis syndrome (aeds) commonly often arises during early infancy. in several intervention studies a beneficial influence on aeds course of certain intestinal bacteria, administered as 'probiotics', has been described. to evaluate the possible role of the natural intestinal microflora in children with allergic eczema/dermatitis syndrome regarding immediate type hypersensitivity to food allergens, children with food allergy (aaeds, n = 68) have been compared with children withou ... | 2006 | 16618364 |
| epidermal naevus in proteus syndrome showing loss of heterozygosity for an inherited pten mutation. | a 3-year-old boy with proteus syndrome has a novel germline p.y68d mutation of the pten gene inherited from his mother who has cowden syndrome. in addition, dna extracted from curettings of his widespread epidermal naevus shows loss of heterozygosity for this mutation. to our knowledge, this has not been described before. | 2006 | 16704655 |
| [wound care management of ulceration in a palliative care patient]. | the wound care management of a palliative care patient with sinusitis pinoidalis is documented. the treatment is validated by the expert's opinion. there is only little evidence-based treatment of such a lesion. in our experience, photo documentation is helpful in decision-making during the treatment process. in addition, healing of larger defects seems to improve upon frequent and extensive liquid cleansing. | 2006 | 16830251 |
| the anti-microbial activity of electrolysed oxidizing water against microorganisms relevant in veterinary medicine. | standards of the german association of veterinary medicine (dvg) for the evaluation of chemical disinfectants were used to assess the anti-microbial efficacy of electrolysed oxidizing water (eow). enterococcus faecium, mycobacterium avium subspecies avium, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus and candida albicans were exposed to anode eow (ph, 3.0+/-0.1; oxidation-reduction potential (orp), +1100+/-50 mv; free chlorine, 400+/-20 mg/l cl2) and combined eow (7:3 anode:c ... | 2006 | 16629725 |
| [a puzzle of the physician and the patient]. | 2006 | 16835961 | |
| [clinical effect of continuous infusion of meropenem on bacterial pneumonia in the elderly]. | we studied the clinical effect of continuous infusion over 24 hours of meropenem (mepm) on bacterial pneumonia in the elderly (over 65). the subjects were 26 patients (community-acquired pneumonia: moderate, n = 9; severe, n= 4; hospital-acquired pneumonia: group iii, n = 13) whose performance status was 3 or 4. mepm 1.0g/day was infused continuously for 7-14 days, and its clinical efficacy, bacteriological efficacy, and side effects were examined prospectively. it was effective in 23 of the 26 ... | 2006 | 16841713 |
| the mechanism of plasmid curing in bacteria. | bacterial plasmids have a major impact on metabolic function. lactose fermentation of e. coli or hemolysin b transporter expressed by the plasmids that carry these respective genes could be readily obviated by heterocyclic compounds that readily bind to plasmid dna. these compounds could also reverse the resistance to antibiotics of e. coli, enterobacter, proteus, staphylococcus and yersinia strains by eliminating plasmids. however, the frequency and extent of this effect was significantly less ... | 2006 | 16842214 |
| lipid-specific membrane activity of human beta-defensin-3. | defensins represent a major component of innate host defense against bacteria, fungi, and enveloped viruses. one potent defensin found, e.g., in epithelia, is the polycationic human beta-defensin-3 (hbd3). we investigated the role of the lipid matrix composition, and in particular the presence of negatively charged lipopolysaccharides (lps) from sensitive (escherichia coli, salmonella enterica serovar minnesota) or resistant (proteus mirabilis) gram-negative bacteria or of the zwitterionic phosp ... | 2006 | 16634647 |