Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| safety and long-term effect of the probiotic fk-23 in patients with hepatitis c virus infection. | a clinical trial was conducted on 39 adult hcv-positive subjects to determine the safety and long-term effect of the probiotic fk-23 (heat-treated enterococcus faecalis strain fk-23). asymptomatic anti-hcv positive adults who fulfilled the selection criteria and gave voluntary consent were recruited from attendees of the hepatitis carrier clinic, department of medical research (lower myanmar). each subject was given 2,700 mg of fk-23 per day by oral route. blood samples were taken at enrollment ... | 2016 | 27508113 |
| identification of aminoglycoside resistance genes by triplex pcr in enterococcus spp. isolated from icus. | early detection of antibiotic-resistant enterococci is an important part of patient treatment. therefore, the aim of the present study was to evaluate the resistance patterns and simultaneously identify and characterise the resistance genes in enterococcus spp. using a triplex polymerase chain reaction (pcr) method. in all, 150 consecutive enterococcus spp were collected from several hospitals in tehran (iran) from january to december 2015. the enterococcus species were identified by standard ph ... | 2016 | 27668903 |
| investigation of c-5 alkynyl (alkynyloxy or hydroxymethyl) and/or n-3 propynyl substituted pyrimidine nucleoside analogs as a new class of antimicrobial agents. | the resurgence of mycobacterial infections and the emergence of drug-resistant strains urgently require a new class of agents that are distinct than current therapies. a group of 5-ethynyl (6-10), 5-(2-propynyloxy) (16, 18, 20, 22, 24), 5-(2-propynyloxy)-3-n-(2-propynyl) (17, 19, 21, 23, 25) and 5-hydroxymethyl-3-n-(2-propynyl) (30-33) derivatives of pyrimidine nucleosides were synthesized and evaluated against mycobacteria [mycobacterium tuberculosis (mtb), mycobacterium bovis (bcg) and mycobac ... | 2016 | 27665179 |
| antibiotic resistance patterns and genetic relatedness of enterococcus faecalis and e. faecium isolated from military working dogs in korea. | enterococcus spp. are normally present in the gastrointestinal tracts of animals and humans, but can cause opportunistic infections that can be transmitted to other animals or humans with integrated antibiotic resistance. to investigate whether this is a potential risk in military working dogs (mwds), we analyzed antibiotic resistance patterns and genetic relatedness of enterococcus spp. isolated from fecal samples of mwds of four different age groups. the isolation rates of enterococcus spp., a ... | 2016 | 27659717 |
| assessment of correlation between clinical parameters and pulp canal pathogens in endodontic pathologies: a microbiological study. | success and failure of root canal therapy is largely dependent on the clinician and the patient. once the tooth is restored into its functional and esthetic place, the root canal therapy is considered to be complete. it is a well-known fact that root canal space acts as a rich reservoir for microorganisms. past studies show that root canal harbors an array of different microorganisms. streptococcus mitis and enterococcus faecalis have been found to be the most prevalent microorganisms isolated f ... | 2016 | 27659083 |
| antibacterial activity of leaf extract of annona muricata and simarouba glauca on enterococcus faecalis. | to determine the antimicrobial effect of water extracts of leaves of annona muricata and simarouba glauca on enterococcus faecalis using agar diffusion method. | 2016 | 27659082 |
| an invitro evaluation of antimicrobial efficacy and flow characteristics for ah plus, mta fillapex, crcs and gutta flow 2 root canal sealer. | cleaning and shaping of root canal does not guarantee complete disinfection of the canal, regardless of the systems and techniques used for this purpose. therefore, it becomes mandatory for the endodontic filling materials to have a good antimicrobial potential especially against e. faecalis. this research was aimed therefore to evaluate the antibacterial efficacy and flow properties of different root canal sealers. | 2016 | 27656550 |
| effect of bacteremia in elderly patients with urinary tract infection. | the clinical effect of bacteremia on outcomes in urinary tract infection (uti) is still debated. this study aims to examine the clinical effect of bacteremia in elderly patients with uti requiring hospital admission. | 2016 | 27650231 |
| prevalence of virulence factors and vancomycin-resistant genes among enterococcus faecalis and e. faecium isolated from clinical specimens. | the aim of this study was to determine the occurrence of virulence determinants and vancomycin-resistant genes among enterococcus faecalis and e. faecium obtained from various clinical sources. | 2016 | 27648425 |
| nationwide surveillance of novel oxazolidinone resistance gene optra in enterococcus isolates in china from 2004 to 2014. | a total of 2,201 nonduplicate enterococcal isolates collected from 29 hospitals in 23 cities in china between 2004 and 2014 were screened for the oxazolidinone resistance gene optra; 45 isolates (2.0%) were optra positive with 11 optra variants identified. the positive rate of optra increased from 0.4% to 3.9% during the 10-year surveillance period. pulsed-field gel electrophoresis (pfge) and multilocus sequence type (mlst) analysis revealed that 37 optra-positive enterococcus faecalis isolates ... | 2016 | 27645239 |
| role of interspecies interactions in dual-species biofilms developed in vitro by uropathogens isolated from polymicrobial urinary catheter-associated bacteriuria. | most catheter-associated urinary tract infections are polymicrobial. here, uropathogen interactions in dual-species biofilms were studied. the dual-species associations selected based on their prevalence in clinical settings were klebsiella pneumoniae-escherichia coli, e. coli-enterococcus faecalis, k. pneumoniae-e. faecalis, and k. pneumoniae-proteus mirabilis. all species developed single-species biofilms in artificial urine. the ability of k. pneumoniae to form biofilms was not affected by e. ... | 2016 | 27642801 |
| the in vitro effect of antimicrobial photodynamic therapy with indocyanine green on enterococcus faecalis: influence of a washing vs non-washing procedure. | the purpose of this study was to evaluate the in vitro effect of washing and non-washing of indocyanine green (icg) as photosensitizer (ps) on bacterial count, biofilm formation, development and degradation of enterococcus faecalis. | 2016 | 27640733 |
| phage therapy against enterococcus faecalis in dental root canals. | antibiotic resistance is an ever-growing problem faced by all major sectors of health care, including dentistry. recurrent infections related to multidrug-resistant bacteria such as methicillin-resistant staphylococcus aureus, carbapenem-resistant enterobacteriaceae, and vancomycin-resistant enterococci (vre) in hospitals are untreatable and question the effectiveness of notable drugs. two major reasons for these recurrent infections are acquired antibiotic resistance genes and biofilm formation ... | 2016 | 27640530 |
| vana-targeted oligonucleotide dna probe designed to monitor vancomycin- and teicoplanin-resistant bacteria in surface waters. | the glycopeptide antibiotics teicoplanin and vancomycin are common to treat severe gram-positive bacterial infections. the gene vana confers high-level resistance to these antibiotics, and these phenomena have been shown to be transferable. release of vancomycin- and teicoplanin-resistant bacteria to surface waters is, therefore, of particular concern since they might proliferate and spread in different environments. monitoring of the fate of vana gene in these waters provides information on the ... | 2016 | 27640164 |
| antimicrobial resistance and virulence profiles of enterococcus spp. isolated from horses in korea. | antimicrobial-resistant (ar) enterococci have emerged as leading nosocomial pathogens. transmission of ar enterococci from animals to humans has been demonstrated. however, there is limited information on the transmission of enterococci from horses to humans. to address this issue, we characterized 260 enterococci isolated from horse-associated samples in korea in 2013 based on their ar profiles and virulence traits. ar profiling revealed an average ratio of ar enterococci of 23.8%. seven isolat ... | 2016 | 27638114 |
| complete genome sequence of a new enterococcus faecalis bacteriophage, vb_efas_ime197. | we report here the whole-genome sequence of a new enterococcus faecalis phage, vb_efas_ime197, which has a linear double-stranded dna genome of 41,307 bp with 34% g+c content. we describe the main features of the genome of vb_efas_ime197. | 2016 | 27634987 |
| the endolysin from the enterococcus faecalis bacteriophage vd13 and conditions stimulating its lytic activity. | bacteriophage produce endolysins (peptidoglycan hydrolases) to lyse the host cell from within and release nascent bacteriophage particles. recombinant endolysins can lyse gram-positive bacteria when added exogenously. as a potential alternative antimicrobial, we cloned and expressed the enterococcal vd13 bacteriophage endolysin. vd13 endolysin has a chap catalytic domain with 92% identity with the bacteriophage ime-ef1 endolysin. the predicted size of vd13 endolysin is ∼27 kda as verified by sds ... | 2016 | 27634307 |
| immunomodulatory and antimicrobial activity of babassu mesocarp improves the survival in lethal sepsis. | attalea speciosa syn orbignya phalerata mart. (babassu) has been used in the treatment of inflammatory and infectious diseases. aim of the study. to investigate the antimicrobial and immunological activity of babassu mesocarp extract (ee). material and methods. the in vitro antimicrobial activity was evaluated by disk diffusion assay and by determination of the minimum inhibitory concentration (mic) to escherichia coli, pseudomonas aeruginosa, enterococcus faecalis, staphylococcus aureus, and me ... | 2016 | 27630733 |
| functional gold nanoparticle-based antibacterial agents for nosocomial and antibiotic-resistant bacteria. | medical treatments for bacterial-infections have become challenging because of the emergence of antibiotic-resistant bacterial strains. thus, new therapeutics and antibiotics must be developed. | 2016 | 27622499 |
| therapeutic potential of gramicidin s in the treatment of root canal infections. | an intrinsic clindamycin-resistant enterococcus faecalis, the most common single species present in teeth after failed root canal therapy, often possesses acquired tetracycline resistance. in these cases, root canal infections are commonly treated with ledermix(®) paste, which contains demeclocycline, or the new alternative endodontic paste odontopaste, which contains clindamycin; however, these treatments are often ineffective. we studied the killing activity of the cyclic antimicrobial peptide ... | 2016 | 27618065 |
| superior antibacterial activity of glcn-aunp-go by ultraviolet irradiation. | a complete bacterialysis analysis of glucosamine-gold nanoparticle-graphene oxide (glcn-aunp-go) and uv-irradiated glcn-aunp-go was conducted. analytical characterization of glcn-aunps, go and glcn-aunp-go revealed uv-vis absorbance peak at around 230 and 500nm. microscopic characterization of prepared nanomaterials was performed by scanning electron microscope, atomic force microscopy, and high-resolution transmission microscopy. the results confirmed that the glcn-aunps were uniformly decorate ... | 2016 | 27612724 |
| longitudinal (2001-14) analysis of enterococci and vre causing invasive infections in european and us hospitals, including a contemporary (2010-13) analysis of oritavancin in vitro potency. | the objective of this study was to evaluate the prevalence and in vitro susceptibility of enterococci and vre among bloodstream infections in european and us hospitals over time. | 2016 | 27609052 |
| in vitro activities of tedizolid compared with other antibiotics against gram-positive pathogens associated with hospital-acquired pneumonia, skin and soft tissue infection and bloodstream infection collected from 26 hospitals in china. | to evaluate the in vitro antimicrobial activities of tedizolid, linezolid and other comparators against clinically significant gram-positive cocci isolates from hospital-acquired pneumonia (hap), skin and soft tissue infection (ssti) and bloodstream infection (bsi), 2140 nonduplicate isolates (23.7 % isolated from hap, 46.8 % from ssti and 29.5 % from bsi) were consecutively collected in 26 hospitals in 17 cities across china during 2014. these pathogens included 632 methicillin-resistant staphy ... | 2016 | 27599998 |
| high-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays. | methods to rapidly assess cell growth would be useful for many applications, including drug susceptibility testing, but current technologies have limited sensitivity or throughput. here we present an approach to precisely and rapidly measure growth rates of many individual cells simultaneously. we flow cells in suspension through a microfluidic channel with 10-12 resonant mass sensors distributed along its length, weighing each cell repeatedly over the 4-20 min it spends in the channel. because ... | 2016 | 27598230 |
| [antibacterial and residual antimicrobial activities of five final irrigants in infected root canal: an in vitro comparative study]. | to evaluate antibacterial and residual antimicrobial activities of five root canal irrigants including qmix, mtad(mixture of a tetracycline isomer, an acid, and a detergent), 0.2% cetrimide(ctr), 2% chlorhexidine(chx) and 17% ethylene diaminetetraacetic acid(edta) and to find the most optimal final irrigants for using in root canal therapy. | 2016 | 27596342 |
| effect of fiber insertion depth on antibacterial efficacy of photodynamic therapy against enterococcus faecalis in rootcanals. | this in vitro study evaluated the effect of fiber insertion depth on antimicrobial efficacy of antimicrobial photodynamic therapy (apdt) using a photosensitizer (ps; toluidine blue) and a red light-emitting diode (led) in root canals infected with enterococcus faecalis. | 2016 | 27591860 |
| antimicrobial and antibiofilm activity of baccharis psiadioides essential oil against antibiotic-resistant enterococcus faecalis strains. | baccharis psiadioides (less.) joch. müller (asteraceae) is considered as a producer of bioactive essential oils and is used in south brazilian folk medicine for its proprieties as stimulant, antipyretic, anti-inflammatory and as an antidote for snake bites. | 2016 | 27590861 |
| microbicidal effects of plain soap vs triclocarban-based antibacterial soap. | the aim of this study was to determine the bactericidal effects of plain and antibacterial soap. the bactericidal effects of plain and antibacterial soap containing 0.3% triclocarban were examined against 10 gram-positive and 10 gram-negative bacterial strains after exposure at 22°c and 40°c for 20 s. gram-negative bacteria were more susceptible to both soaps than gram-positive bacteria. however, with one exception (enterococcus faecalis atcc 19433 at 40°c), there was no significant difference b ... | 2016 | 27585555 |
| effect of different mixing methods on the bacterial microleakage of calcium-enriched mixture cement. | calcium-enriched mixture (cem) cement is used in the field of endodontics. it is similar to mineral trioxide aggregate in its main ingredients. the present study investigated the effect of different mixing methods on the bacterial microleakage of cem cement. | 2016 | 27580651 |
| molecular basis for lytic bacteriophage resistance in enterococci. | the human intestine harbors diverse communities of bacteria and bacteriophages. given the specificity of phages for their bacterial hosts, there is growing interest in using phage therapies to combat the rising incidence of multidrug-resistant bacterial infections. a significant barrier to such therapies is the rapid development of phage-resistant bacteria, highlighting the need to understand how bacteria acquire phage resistance in vivo here we identify novel lytic phages in municipal raw sewag ... | 2016 | 27578757 |
| genome-based characterization of hospital-adapted enterococcus faecalis lineages. | vancomycin-resistant enterococcus faecalis (vrefs) is an important nosocomial pathogen(1,2). we undertook whole genome sequencing of e. faecalis associated with bloodstream infection in the uk and ireland over more than a decade to determine the population structure and genetic associations with hospital adaptation. three lineages predominated in the population, two of which (l1 and l2) were nationally distributed, and one (l3) geographically restricted. genome comparison with a global collectio ... | 2016 | 27572164 |
| comparative evaluation of antimicrobial efficacy of three herbal irrigants in reducing intracanal e. faecalis populations: an in vitro study. | the present study aimed to evaluate the intracanal bacterial reduction promoted by chemomechanical preparation using three different herbal extracts named ocimum sanctum (os), cinnamomum zeylanicum (cz), syzygium aromaticum (sa) against enterococcus faecalis. | 2016 | 27398170 |
| activity of the lichen usnea steineri and its major metabolites against gram-positive, multidrug-resistant bacteria. | the antimicrobial activity and possible synergistic effects of extracts and compounds isolated from usnea steineri were evaluated against four resistant bacterial species. a phytochemical study of the acetone extract of u. steineri resulted in the isolation and characterization of difractaic acid and (+)-usnic acid as the main compounds. the acetone extract showed strong activity (less than 10 µg/ml) against resistant strains of staphylococcus epidermidis and enterococcus faecalis, and (+)-usnic ... | 2016 | 27396202 |
| rose bengal uptake by e. faecalis and f. nucleatum and light-mediated antibacterial activity measured by flow cytometry. | antibacterial photodynamic therapy (apdt) using rose bengal (rb) and blue-light kills bacteria through the production of reactive oxygen derivates. however, the interaction mechanism of rb with bacterial cells remains unclear. this study investigated the uptake efficiency and the antibacterial activity of blue light-activated rb against enterococcus faecalis and fusobacterium nucleatum. spectrophotometry and epifluorescence microscopy were used to evaluate binding of rb to bacteria. the antibact ... | 2016 | 27394008 |
| assessment of free fatty acids and cholesteryl esters delivered in liposomes as novel class of antibiotic. | healthcare associated infections (hai) with multidrug-resistant (mdr) bacteria continue to be a global threat, highlighting an urgent need for novel antibiotics. in this study, we assessed the potential of free fatty acids and cholesteryl esters that form part of the innate host defense as novel antibacterial agents for use against mdr bacteria. | 2016 | 27391402 |
| the impact of hospital and urban wastewaters on the bacteriological contamination of the water resources in kinshasa, democratic republic of congo. | although the united nations general assembly recognized in 2010 the right to safe and clean drinking water and sanitation as a human right that is essential to the full enjoyment of life and all other human rights, the contamination of water supplies with faecal pathogens is still a major and unsolved problem in many parts of the world. in this study, faecal indicator bacteria (fib), including escherichia coli (e. coli) and enterococcus (ent), were quantified over the period of june/july 2014 an ... | 2016 | 27389829 |
| probiotic activity of enterococcus faecalis cect7121: effects on mucosal immunity and intestinal epithelial cells. | to analyse the effect of enterococcus faecalis cect7121 on intestinal epithelial cells (iecs) and its effects on the mucosal immune response. | 2016 | 27389465 |
| the ctsr regulator controls the expression of clpc, clpe and clpp and is required for the virulence of enterococcus faecalis in an invertebrate model. | the intrinsic ruggedness of enterococcus faecalis is responsible for its widespread distribution in nature and is often viewed as an important virulence determinant. previously, we showed that the clpb atpase is negatively regulated by ctsr and is required for thermotolerance and virulence in a galleria mellonella invertebrate model. here, we used in silico, northern blot and quantitative real-time pcr analyses to identify additional members of the ctsr regulon, namely the clpp peptidase and the ... | 2016 | 27388279 |
| evaluation of the antibacterial efficacy of azadirachta indica, commiphora myrrha, glycyrrhiza glabra against enterococcus faecalis using real time pcr. | to compare the antibacterial efficacy of azadirachta indica (neem), commiphora myrrha (myrrh), glycyrrhiza glabra (liquorice) with 2% chlorhexidine (chx) against e. faecalis by using real time pcr. | 2016 | 27386000 |
| the heterodimeric abc transporter efrcd mediates multidrug efflux in enterococcus faecalis. | nosocomial infections with enterococcus faecalis are an emerging health problem. however, drug efflux pumps contributing to intrinsic drug resistance are poorly studied in this gram-positive pathogen. in this study, we functionally investigated seven heterodimeric abc transporters of e. faecalis that are annotated as drug efflux pumps. deletion of ef0789-ef0790 on the chromosome of e. faecalis resulted in increased susceptibility to daunorubicin, doxorubicin, ethidium, and hoechst 33342, and the ... | 2016 | 27381387 |
| inhibition of enterococcus faecalis by calcium peroxide. | to investigate the inhibition of enterococcus faecalis by calcium peroxide (cao₂). | 2016 | 27379349 |
| photodynamic inactivation of root canal bacteria by light activation through human dental hard and simulated surrounding tissue. | photodynamic inactivation of bacteria (pib) may be a supportive antimicrobial approach for use in endodontics, but sufficient activation of photosensitizers (ps) in root canals is a critical point. therefore, aim of this study was to evaluate the ability of ps absorbing blue (tmpyp) or red light (methylene blue; mb) for light activation through human dental hard and simulated surrounding tissue to inactivate root canal bacteria. | 2016 | 27379059 |
| the effect of the final irrigant on the antimicrobial activity of root canal sealers. | root canal irrigation is an essential step in root canal therapy as it enables the elimination of microorganisms. the final irrigant may affect the properties of the root canal sealer used during obturation particularly with tricalcium silicate-based sealers, which interact with dentine. the aim of this study was to assess the antimicrobial activity of tricalcium silicate-containing sealers in contact with different irrigants. furthermore the sealer surface in contact with the irrigant was chara ... | 2016 | 27377571 |
| [chronic bacterial prostatitis. clinical and microbiological study of 332 cases]. | chronic bacterial prostatitis (cbp) is characterized by long-lasting symptoms, frequently associated with psychosomatic disorders. the objective of the study was to study pcb in our environment clinically and microbiologically. | 2016 | 27377215 |
| a large factory-scale application of selected autochthonous lactic acid bacteria for pdo pecorino siciliano cheese production. | the main hypothesis of this study was that the autochthonous lactic acid bacteria (lab) selected for their dairy traits are able to stabilize the production of pdo (protected denomination of origin) pecorino siciliano cheese, preserving its typicality. the experimental plan included the application of a multi-strain lactic acid bacteria (lab) culture, composed of starter (lactococcus lactis subsp. lactis cag4 and cag37) and non starter (enterococcus faecalis psl71, lactococcus garviae psl67 and ... | 2016 | 27375245 |
| disinfection efficacy of current regenerative endodontic protocols in simulated necrotic immature permanent teeth. | the lack of mechanical debridement and reduced concentrations suggested for chemical debridement to maintain stem cell viability call into question the disinfection efficacy of current regenerative protocols. current protocols vary in the concentration and type of antibiotic medicaments used. the aim of this study was to determine if simulated immature teeth infected with enterococcus faecalis can be completely disinfected by following current standardized regenerative protocols and to evaluate ... | 2016 | 27372159 |
| antimicrobial resistance of escherichia coli, enterococci, pseudomonas aeruginosa, and staphylococcus aureus from raw fish and seafood imported into switzerland. | a total of 44 samples of salmon, pangasius (shark catfish), shrimps, and oysters were tested for the presence of escherichia coli, enterococci, pseudomonas aeruginosa, and staphylococcus aureus, which are indicator organisms commonly used in programs to monitor antibiotic resistance. the isolated bacterial strains, confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy, were tested against a panel of 29 antimicrobial agents to obtain mics. across the four sampl ... | 2016 | 27357045 |
| partial diversity generates effector immunity specificity of the bac41-like bacteriocins of enterococcus faecalis clinical strains. | bacteriocin 41 (bac41) is the plasmid-encoded bacteriocin produced by the opportunistic pathogen enterococcus faecalis its genetic determinant consists of bacl1 (effector), bacl2 (regulator), baca (effector), and baci (immunity). the secreted effectors bacl1 and baca coordinate to induce the lytic cell death of e. faecalis meanwhile, the immunity factor baci provides self-resistance to the bac41 producer, e. faecalis, against the action of bacl1 and baca. in this study, we demonstrated that more ... | 2016 | 27353651 |
| activities of tedizolid and linezolid determined by the reference broth microdilution method against 3,032 gram-positive bacterial isolates collected in asia-pacific, eastern europe, and latin american countries in 2014. | tedizolid and linezolid in vitro activities against 3,032 gram-positive pathogens collected in asia-pacific, eastern european, and latin american medical centers during 2014 were assessed. the isolates were tested for susceptibility by the current reference broth microdilution methods. due to concern over the effect of mic endpoint criteria on the results of testing the oxazolidinones tedizolid and linezolid, mic endpoint values were read by two methods: (i) reading the mic at the first well whe ... | 2016 | 27353270 |
| identification of bacterial species by untargeted nmr spectroscopy of the exo-metabolome. | identification of bacterial species is a crucial bottleneck for clinical diagnosis of infectious diseases. quick and reliable identification is a key factor to provide suitable antibiotherapies and avoid the development of multiple-drug resistance. we propose a novel nuclear magnetic resonance (nmr)-based metabolomics strategy for rapid discrimination and identification of several bacterial species that relies on untargeted metabolic profiling of supernatants from bacterial culture media. we sho ... | 2016 | 27349704 |
| a new buildup biofilm model that mimics accumulation of material in flexible endoscope channels. | the objective of this study was to develop a new build up biofilm (bbf) model that was based on repeated exposure to test soil containing enterococcus faecalis and pseudomonas aeruginosa and repeated rounds of fixation to mimic the accumulation of patient material in endoscope channels during reprocessing. the new bbf model is a novel adaptation of the minimum biofilm effective concentration (mbec) 96-well model where biofilm is formed on plastic pegs. the new mbec-bbf model was developed over e ... | 2016 | 27345713 |
| residual antibiofilm effects of various concentrations of double antibiotic paste used during regenerative endodontics after different application times. | we investigated the residual antibiofilm effects of different concentrations of double antibiotic paste (dap) applied on radicular dentin for 1 or 4 weeks. | 2016 | 27341460 |
| eggshells as a source for occupational exposure to airborne bacteria in hatcheries. | occupational exposure to high concentrations of airborne bacteria in poultry production is related to an increased risk of respiratory disorders. however, potential sources and formation of hatchery bioaerosols are rarely characterized. in this study, bacterial multiplication on fresh shell fragments from turkey hatching eggs under conditions present in a hatcher incubator was investigated. a 10(5)-fold amplification was observed both by colony count and total cell count gaining 4 × 10(7) cfu/ce ... | 2016 | 27341051 |
| draft genome sequence for a clinical isolate of vancomycin-resistant enterococcus faecalis. | we report here the draft genome sequence of a multidrug-resistant enterococcus faecalis strain, isolated from a patient at the university of colorado hospital. the genome assembly is 3,040,186 bp in length with 37.6% gc content. this isolate encodes eleven resistance genes, including those for glycopeptide, aminoglycoside, macrolide-lincosamide-streptogramin, and tetracycline resistance. | 2016 | 27340066 |
| can antimicrobial photodynamic therapy (apdt) enhance the endodontic treatment? | in order to achieve a long-lasting effect, one of the main goals in root canal treatment is to eliminate the endodontic bacteria. conventional chemomechanical debridement is considered as the basic treatment in root canal therapy, but adjunctive techniques such as antimicrobial photodynamic therapy (apdt) can also be helpful. the aim of this study was to evaluate reports in the scientific literature that used different photosensitizers (pss) for bacterial reduction. the literature search was con ... | 2016 | 27330702 |
| an evolved oxazolidinone with selective potency against mycobacterium tuberculosis and gram positive bacteria. | innovation of new antibacterials that are effective against strains that have developed resistance to existing drugs would strengthen our ability to treat and subsequently control spread of pathogenic bacteria. increasing incidence of infections with drug resistant bacteria has become a common occurrence in recent times. we have developed an evolved oxazolidinone, t145, which inhibits growth of enterococcus faecalis, staphylococcus aureus and mycobacterium tuberculosis (mtb) with sub μg/ml poten ... | 2016 | 27329794 |
| sub-lethal doses of photodynamic therapy affect biofilm formation ability and metabolic activity of enterococcus faecalis. | during photodynamic therapy (pdt) in the treatment of a primary endodontic infection, it is extremely likely that microorganisms would be exposed to sub-lethal doses of pdt (spdt). although spdt cannot kill microorganisms, it can considerably influence microbial virulence. this study was conducted to characterize the effect of spdt using toluidine blue o (tbo), methylene blue (mb), and indocyanine green (icg) on biofilm formation ability and metabolic activity of enterococcus faecalis. | 2016 | 27328452 |
| enzymatic synthesis using immobilized enterococcus faecalis esawy dextransucrase and some applied studies. | dextrans enzymatic synthesis by immobilized enterococcus faecalis esawy dextransucrase was studied. different parameters, such as: enzyme protein concentration (epc), substrate concentration (sc), temperature and reaction time were evaluated. epc played a fundamental role in controlling dextran molecular size with 0.1% dextran in reaction mixture. dextran 38,397 and 125,471da were yielded at epc 4.78 and 5.78mg, respectively. proper dextrans (73,378 and 117,521da) demanded in pharmaceutical appl ... | 2016 | 27327909 |
| enterococcus faecalis lipoteichoic acid-induced nlrp3 inflammasome via the activation of the nuclear factor kappa b pathway. | we wished to examine the effects of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) inflammasome on periapical periodontitis induced by enterococcus faecalis and to investigate the molecular mechanisms of lipoteichoic acid (lta) derived from e. faecalis on the expression of the nlrp3 inflammasome. | 2016 | 27325457 |
| study of antibacterial mechanism of graphene oxide using raman spectroscopy. | graphene oxide (go) is extensively proposed as an effective antibacterial agent in commercial product packaging and for various biomedical applications. however, the antibacterial mode of action of go is yet hypothetical and unclear. here we developed a new and sensitive fingerprint approach to study the antibacterial activity of go and underlying mechanism, using raman spectroscopy. spectroscopic signatures obtained from biomolecules such as adenine and proteins from bacterial cultures with dif ... | 2016 | 27324288 |
| evaluation of the susceptibility of multispecies biofilms in dentinal tubules to disinfecting solutions. | the present study evaluated the effect of the source of biofilm bacteria on their susceptibility in dentinal tubules to disinfecting solutions using an infected dentin model. | 2016 | 27318625 |
| comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents. | we compared chromid vre and brilliance vre media for the detection of vancomycin-resistant enterococci (vre). using a panel of 28 enterococcal isolates, 10 vana enterococcus faecium and three vana enterococcus faecalis isolates grew as per manufacturers' instructions whilst growth of two vanc and eight vancomycin-susceptible enterococci was inhibited on both media. important differences were noted in the selectivity and chromogenic properties of the two media for vana enterococcus raffinosus and ... | 2016 | 27312688 |
| evaluation of the in vitro growth of urinary tract infection-causing gram-negative and gram-positive bacteria in a proposed synthetic human urine (shu) medium. | bacteriuria is a hallmark of urinary tract infection (uti) and asymptomatic bacteriuria (abu), which are among the most frequent infections in humans. a variety of gram-negative and gram-positive bacteria are associated with these infections but escherichia coli contributes up to 80% of cases. multiple bacterial species including e. coli can grow in human urine as a means to maintain colonization during infections. in vitro bacteriuria studies aimed at modeling microbial growth in urine have uti ... | 2016 | 27312379 |
| ex situ study of enterococcus faecalis survival in the recreational waters of the southern coast of the caspian sea. | the us environmental protection agency has suggested faecal enterococci as the primary bacterial indicators. of more importance is their direct correlation with swimmer-associated gastroenteritis in recreation water quality monitoring. in contrast to other seawater bodies with 3.5% salinity, the recreational waters in the southern coast of the caspian sea possess its own salinity (about 1% w/v) and thus require further investigations to determine the capacity of enterococcus faecalis as the sole ... | 2016 | 27307975 |
| risk factors of endocarditis in patients with enterococcus faecalis bacteremia: external validation of the nova score. | the nova score is a recently developed diagnostic tool used to identify patients with increased risk of infective endocarditis (ie) among patients with enterococcus faecalis bacteremia. we aimed to validate the nova score and to identify risk factors for ie. | 2016 | 27307506 |
| global emergence and dissemination of enterococci as nosocomial pathogens: attack of the clones? | enterococci are gram-positive bacteria that are found in plants, soil and as commensals of the gastrointestinal tract of humans, mammals, and insects. despite their commensal nature, they have also become globally important nosocomial pathogens. within the genus enterococcus, enterococcus faecium, and enterococcus faecalis are clinically most relevant. in this review, we will discuss how e. faecium and e. faecalis have evolved to become a globally disseminated nosocomial pathogen. e. faecium has ... | 2016 | 27303380 |
| postoperative infectious complications in patients undergoing holmium laser enucleation of the prostate: risk factors and microbiological analysis. | to examine the incidence of postoperative bacteriuria and febrile complications, and to investigate bacterial strains in the urine of patients undergoing holmium laser enucleation of the prostate. | 2016 | 27302684 |
| molecular analysis of vancomycin-resistant enterococci isolated from regional hospitals in trinidad and tobago. | geographic spread of vancomycin-resistant enterococci (vre) clones in cities, countries, or even continents has been identified by molecular techniques. this study aimed at characterizing virulent genes and determining genetic relatedness of 45 vre isolates from trinidad and tobago using molecular tools, including polymerase chain reaction, pulsed-field gel electrophoresis (pfge), and random amplification polymorphic dna (rapd). the majority (84%) of the isolates were enterococcus faecium posses ... | 2016 | 27299153 |
| the antimicrobial efficacy and dna binding activity of the copper(ii) complexes of 3,4,7,8-tetramethyl-1,10-phenanthroline, 4,7-diphenyl-1,10-phenanthroline and 1,2-diaminocyclohexane. | four copper(ii) complexes of the general structure [cu(l1)(l2)](2+), where l1 is (1s,2s)-diaminocyclohexane or (1r,2r)-diaminocyclohexane and l2 is 3,4,7,8-tetramethyl-1,10-phenanthroline (tmp) or 4,7-diphenyl-1,10-phenanthroline (dip), have been investigated in this study for their antimicrobial activity, short-term antimicrobial efficacy, and in vitro dna-binding affinity. against an expanded panel of bacterial and fungal strains in 12 species, minimal inhibitory concentrations (mic) for these ... | 2016 | 27293146 |
| tigecycline antimicrobial activity tested against clinical bacteria from latin american medical centres: results from sentry antimicrobial surveillance program (2011-2014). | bacterial organisms (n = 13,494) were consecutively collected in 2011-2014 from 21 latin american medical centres (11 nations). antimicrobial susceptibility was determined by broth microdilution at a central laboratory. tigecycline was very active against gram-positive organisms, with mic50/90 values of 0.06/0.06 µg/ml for staphylococcus aureus (n = 2878), 0.06/0.12 µg/ml for coagulase-negative staphylococci (n = 880), 0.06/0.06 µg/ml for enterococci (n = 708) and ≤0.03/≤0.03-0.06 µg/ml for stre ... | 2016 | 27291285 |
| fishing for gutta-percha-adhered biofilms in purulent post-treatment apical periodontitis. | this study investigated the possibility of depicting individual taxa in clinically relevant biofilms using fluorescent in situ hybridization (fish). gutta-percha samples were collected from the apical aspect of root canals associated with a chronic apical abscess (test samples, n = 8). corresponding control samples were obtained from previously filled root canals with apparently normal periapical tissues (n = 3). the transport medium was investigated for detached biofilm fragments using fish sta ... | 2016 | 27284969 |
| physicochemical characterisation and antimicrobial phototoxicity of an anionic porphyrin in natural deep eutectic solvents. | natural deep eutectic solvents (nades) are a newly discovered group of eutectics which has shown promise as a solvent in antimicrobial photodynamic therapy (apdt). the purpose of this study was to investigate preparations of an anionic porphyrin, meso-tetra-(4-carboxyphenyl)-porphine (tcpp), solubilised in nades, with regard to their physicochemical and antibacterial properties. the nades cs (ph∼0), chx (ph∼4) and mfg (ph∼1) solubilised tcpp with absorption maximum ∼443nm and emission maximum ∼6 ... | 2016 | 27269504 |
| neofiscalin a and fiscalin c are potential novel indole alkaloid alternatives for the treatment of multidrug-resistant gram-positive bacterial infections. | ten indole alkaloids were obtained from the marine sponge-associated fungus neosartorya siamensis kufa 0017. we studied the antimicrobial properties of these and of three other compounds previously isolated from the soil fungus n. siamensis kufc 6349. only neofiscalin a showed antimicrobial activity against gram-positive bacteria, including methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus faecalis (vre); with a minimum inhibitory concentration (mic) of 8 μ ... | 2016 | 27268269 |
| study of the impact of cranberry extract on the virulence factors and biofilm formation by enterococcus faecalis strains isolated from urinary tract infections. | drinking of cranberry fruit juice and application of commercial preparations containing the cranberry extracts are recommended in the prevention and treatment of urinary tract infections (utis), especially in women with recurrent utis. many studies focus on the activity of cranberries against uropathogenic escherichia coli (e. coli) strains. however, the knowledge of the cranberry effect on gram-positive enterococcus faecalis (e. faecalis) is limited. therefore, the aim of our study was to estab ... | 2016 | 27456160 |
| development of pla films containing oregano essential oil (origanum vulgare l. virens) intended for use in food packaging. | consumers' concerns about the environment and health have led to the development of new food packaging materials avoiding petroleum-based matrices and synthetic additives. the present study has developed polylactic acid (pla) films containing different concentrations of essential oil from origanum vulgare l. virens (oeo). the effectiveness of this new active packaging was checked for use in ready-to-eat salads. a plasticising effect was observed when oeo was incorporated in pla films. the rest o ... | 2016 | 27455176 |
| triterpenoids from the leaves of alphitonia xerocarpus baill and their biological activity. | ten previously undescribed triterpenoid saponins and a previously undescribed norlupane triterpenoid were isolated, with three known saponins, four known flavonoids, two known lupane derivatives, sitosterol and 6'-heptadecanoyl-3-o-β-d-glucopyranosylsitosterol from the leaves of alphitonia xerocarpus (rhamnaceae), an endemic tree of new caledonia. the chemical structures of the purified compounds were identified by nuclear magnetic resonance and mass spectrometry. the isolated compounds were tes ... | 2016 | 27452452 |
| antibacterial effectiveness of 2 root canal irrigants in root-filled teeth with infection: a randomized clinical trial. | this study compared the antibacterial effects of 1% sodium hypochlorite (naocl) and 2% chlorhexidine digluconate (chx) during retreatment of teeth with apical periodontitis. | 2016 | 27452293 |
| effect of a genetically engineered bacteriophage on enterococcus faecalis biofilms. | enterococcus faecalis is a gram-positive, facultative anaerobic bacterium that is associated with failed endodontic cases and nosocomial infections. e. faecalis can form biofilms, penetrate dentinal tubules and survive in root canals with scarce nutritional supplies. these properties can make e. faecalis resistant to conventional endodontic disinfection therapy. furthermore, treatment may be complicated by the fact that many e. faecalis strains are resistant to antibiotics. a potential alternati ... | 2016 | 27448990 |
| evaluation of treatment and disinfection of water using cold atmospheric plasma. | in this paper, the disinfection of water is investigated using plasma spark treatment and the results are compared with conventional techniques. inactivation of the enterococcus faecalis and escherichia coli bacteria is considered in the treatment process of water by the plasma spark. for this purpose, many physical and chemical parameters of water are measured and the obtained results demonstrate a reduction of 8-log in colony forming units of e. coli and e. faecalis at 15 minutes and 12 minute ... | 2016 | 27441856 |
| temporal variabilities in genetic patterns and antibiotic resistance profiles of enterococci isolated from human feces. | temporal variabilities in the genetic patterns and antibiotic resistance profiles of enterococci were monitored over a 7-month period. enterococcus faecalis isolates (103 strains) collected from feces showed only one genetic pattern and antibiotic resistance profile within 0 d and 30 d. in contrast, after 60 d and 90 d, the genetic patterns and antibiotic resistance profiles of all e. faecalis isolates (8 strains) clearly differed within 30 d. these results indicate that the genetic patterns and ... | 2016 | 27265342 |
| enterococcus faecalis promotes osteoclast differentiation within an osteoblast/osteoclast co-culture system. | to investigate the effects of heat-killed enterococcus faecalis atcc 29212 and p25rc clinical strain (derived from an obturated root canal with apical periodontitis) on osteoclast differentiation within an osteoblast/osteoclast co-culture system. | 2016 | 27262294 |
| virulence traits and antibiotic resistance among enterococci isolated from dogs with periodontal disease. | periodontal disease - pd - is one of the most widespread diseases in dogs, but the role of this odontogenic infection in the dissemination of pathogenic bacteria present in the oral mucosa to other animals or pet owners is understudied. trying to unveil the putative pathogenicity of enterococci present in the gums of dogs diagnosed with pd, thirty-two animals were investigated during routine visits to a private veterinary clinic. seventy-one enterococci were recovered and characterized regarding ... | 2016 | 27260807 |
| evidence for persistent and shared bacterial strains against a background of largely unique gut colonization in hospitalized premature infants. | the potentially critical stage of initial gut colonization in premature infants occurs in the hospital environment, where infants are exposed to a variety of hospital-associated bacteria. because few studies of microbial communities are strain-resolved, we know little about the extent to which specific strains persist in the hospital environment and disperse among infants. to study this, we compared 304 near-complete genomes reconstructed from fecal samples of 21 infants hospitalized in the same ... | 2016 | 27258951 |
| topical formulation comprising fatty acid extract from cod liver oil: development, evaluation and stability studies. | the purpose of this study was to develop a pharmaceutical formulation containing fatty acid extract rich in free omega-3 fatty acids such as eicosapentaenoic acid and docosahexaenoic acid for topical use. although the health benefits of cod liver oil and other fish oils taken orally as a dietary supplement have been acknowledged and exploited, it is clear that their use can be extended further to cover their antibacterial properties. in vitro evaluation showed that 20% (v/v) fatty acid extract e ... | 2016 | 27258290 |
| antimicrobial and physicochemical characterization of biodegradable, nitric oxide-releasing nanocellulose-chitosan packaging membranes. | biodegradable composite membranes with antimicrobial properties consisting of nanocellulose fibrils (cnfs), chitosan, and s-nitroso-n-acetyl-d-penicillamine (snap) were developed and tested for food packaging applications. as a nitric oxide donor, snap was encapsulated into completely dispersed chitosan in 100 ml of 0.1 n acetic acid and was thoroughly mixed with cnfs to produce a composite membrane. the fabricated membranes had a uniform dispersion of chitosan and snap within the cnfs, which wa ... | 2016 | 27258235 |
| emergence of vana gene among vancomycin-resistant enterococci in a tertiary care hospital of north - east india. | vancomycin-resistant enterococci (vre) have become one of the most challenging nosocomial pathogens with the rapid spread of the multi-drug resistant strain with limited therapeutic options. it is a matter of concern due to its ability to transfer vancomycin resistant gene to other organisms. the present study was undertaken to determine the emergence of vancomycin-resistant enterococci and the vana gene among the isolates in a tertiary care hospital of north-east india. | 2016 | 27241650 |
| antibacterial and antimetastatic potential of diospyros lycioides extract on cervical cancer cells and associated pathogens. | cervical cancer is among the most prevalent forms of cancer in women worldwide. diospyros lycioides was extracted using hexane, ethyl acetate, acetone, and methanol and finger print profiles were determined. the leaf material was tested for the presence of flavonoids, tannins, saponins, terpenoids, and cardiac glycosides using standard chemical methods and the presence of flavonoids and phenolics using thin layer chromatography. the total phenolic content was determined using folin-ciocalteu pro ... | 2016 | 27239210 |
| gram-positive uropathogens, polymicrobial urinary tract infection, and the emerging microbiota of the urinary tract. | gram-positive bacteria are a common cause of urinary-tract infection (uti), particularly among individuals who are elderly, pregnant, or who have other risk factors for uti. here we review the epidemiology, virulence mechanisms, and host response to the most frequently isolated gram-positive uropathogens: staphylococcus saprophyticus, enterococcus faecalis, and streptococcus agalactiae. we also review several emerging, rare, misclassified, and otherwise underreported gram-positive pathogens of t ... | 2016 | 27227294 |
| postneurosurgical central nervous system infection due to enterococcus faecalis successfully treated with intraventricular vancomycin. | infections from enterococcus faecalis and enterococcus faecium are uncommon in the post-neurosurgical intervention setting., [1, 2, 3, 4] intraventricular antibiotics are recommended when standard intravenous therapy fails. [5] here we present a case of post-neurosurgical ventriculitis, meningitis, and cerebritis in an oncology patient caused by refractory enterococcus faecalis successfully treated with intraventricular vancomycin. | 2016 | 27226704 |
| antimicrobial susceptibility and characterization of virulence genes of enterococcus faecalis isolates from teeth with failure of the endodontic treatment. | the aim of this study was to investigate the prevalence of virulence factors and the antimicrobial resistance of enterococcus faecalis isolates of teeth with failure of the endodontic treatment. | 2016 | 27221594 |
| antibacterial efficacy of calcium hypochlorite with vibringe sonic irrigation system on enterococcus faecalis: an in vitro study. | aim. the purpose of this study was to compare the in vitro efficacy of calcium hypochlorite (ca[ocl]2) and sodium hypochlorite (naocl) associated with sonic (vibringe) irrigation system in root canals which were contaminated with enterococcus faecalis. material and methods. the root canals of 84 single-rooted premolars were enlarged up to a file 40, autoclaved, inoculated with enterococcus faecalis, and incubated for 21 days. the samples were divided into 7 groups according to the irrigation pro ... | 2016 | 27218106 |
| increasing prevalence of aminoglycoside-resistant enterococcus faecalis isolates due to the aac(6')-aph(2") gene: a therapeutic problem in kermanshah, iran. | enterococci are important pathogens in nosocomial infections. various types of antibiotics, such as aminoglycosides, are used for treatment of these infections. enterococci can acquire resistant traits, which can lead to therapeutic problems with aminoglycosides. | 2016 | 27217920 |
| vana-type mrsa (vrsa) emerged in surface waters. | due to the widespread occurrence of meca-encoded methicillin resistance in staphylococcus aureus (mrsa), treatment of staphylococcal infections is shifted to glycopeptide antibiotics like vancomycin and teicoplanin. the selective pressure of glycopeptides has eventually led to the emergence of staphylococci with increased resistance. of great concern is vana-encoded high level vancomycin and teicoplanin resistance in mrsa (vrsa). therefore, this study aimed at investigating the occurrence of vrs ... | 2016 | 27216737 |
| genome-based characterization of hospital-adapted enterococcus faecalis lineages. | vancomycin-resistant enterococcus faecalis (vrefs) is an important nosocomial pathogen1,2. we undertook whole genome sequencing of e. faecalis associated with bloodstream infection in the uk and ireland over more than a decade to determine the population structure and genetic associations with hospital adaptation. three lineages predominated in the population, two of which (l1 and l2) were nationally distributed, and one (l3) geographically restricted. genome comparison with a global collection ... | 2016 | 27213049 |
| bactericidal effect of strong acid electrolyzed water against flow enterococcus faecalis biofilms. | this study evaluated the bactericidal effect of strong acid electrolyzed water (saew) against flow enterococcus faecalis biofilm and its potential application as a root canal irrigant. | 2016 | 27208991 |
| antagonistic donor density effect conserved in multiple enterococcal conjugative plasmids. | enterococcus faecalis, a common causative agent of hospital-acquired infections, is resistant to many known antibiotics. its ability to acquire and transfer resistance genes and virulence determinants through conjugative plasmids poses a serious concern for public health. in some cases, induction of transfer of e. faecalis plasmids results from peptide pheromones produced by plasmid-free recipient cells, which are sensed by the plasmid-bearing donor cells. these plasmids generally encode an inhi ... | 2016 | 27208137 |
| exogenous fatty acids protect enterococcus faecalis from daptomycin-induced membrane stress independently of the response regulator liar. | enterococcus faecalis is a commensal bacterium of the gastrointestinal tract that can cause nosocomial infections in immunocompromised humans. the hallmarks of this organism are its ability to survive in a variety of stressful habitats and, in particular, its ability to withstand membrane damage. one strategy used by e. faecalis to protect itself from membrane-damaging agents, including the antibiotic daptomycin, involves incorporation of exogenous fatty acids from bile or serum into the cell me ... | 2016 | 27208105 |
| rare case of simultaneous enterococcal endocarditis and prosthetic joint infection. | a 59-year-old man was admitted with a 3-month history of daily fevers as well as bilateral knee pain and swelling. medical history was significant for bilateral knee arthroplasties 4 years prior to admission. two sets of peripheral blood cultures as well as bilateral knee synovial fluid grew enterococcus faecalis within 10 hours. transoesophageal echocardiography revealed aortic and mitral valve vegetations suggestive of infectious endocarditis, with severe regurgitation secondary to large size. ... | 2016 | 27207984 |
| antimicrobial and cytotoxic activity of cinnamomum zeylanicum, calcium hydroxide, and triple antibiotic paste as root canal dressing materials. | the aim of this article was (i) to define the chemical constituents of cinnamomum zeylanicum essential oil (ceo), (ii) to compare the antimicrobial activity of ceo with triple antibiotic paste (tap) and calcium hydroxide [ca(oh)2] on planktonic and biofilm enterococcus faecalis; and (iii) to compare the cytotoxicity of these medicaments on l929 fibroblasts. | 2016 | 27206997 |
| influence of gelatin-thrombin matrix tissue sealant on bacterial colony formation and risk of pelvic infection. | objective. gelatin-thrombin matrix (gtm) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies. we aim to elucidate contributing factors by assessing influence of gtm on bacterial colony formation and characterizing bacteria present at the vaginal cuff. methods. escherichia coli was incubated in phosphate-buffered saline (pbs) and pelvic washings with and without gtm to assess influence on colony formation. pelvic washings of the va ... | 2016 | 27199534 |