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[xanthogranulomatous pyelonephritis: presentation of an unusual case].xanthogranulomatous pyelonephritis is a morphologically and clinically unique manifestation of chronic pyelonephritis with the formation of pus or granulomas. the most frequent predisposing factors for the development of xanthogranulomatous pyelonephritis are urinary obstruction (e. g., stones, tumors, congenital anomalies and functional impairment) and infection of the collecting system.200516001341
evaluation of cd4(+)/cd8(+) status and urinary tract infections associated with urinary schistosomiasis among some rural nigerians.data on urinary schistosomiasis in nigeria are mainly epidemological. the knowledge of co-infections of urinary schistosomiasis and other pathogens are important epidemiological tools for the control and health benefits of the rural dwellers. the granulomatous reactions in urinary schistosomiasis is cd4(+) dependent. the cd8(+) is cytotoxic to parasites and it is activated by cd4(+). these parameters therefore participate in the immune responses to urinary schistosomiasis.200516006219
analysis of pten gene mutations in korean patients with cowden syndrome and polyposis syndrome.pten (phosphatase and tensin homologue deleted in chromosome 10) is a candidate tumor suppressor gene. mutations of this gene are responsible for pten hamartoma tumor syndromes, including cowden syndrome, bannayan-riley-ruvalcaba syndrome, proteus syndrome, and proteus -like syndromes. recently, pten mutations were identified in several human neoplasms. we analyzed the dna of various organs and lesions in korean patients with cowden syndrome, their family members, and patients with familial aden ...200516007494
etiological and clinical patterns of childhood urolithiasis in iraq.this study was conducted to evaluate the etiological and clinical characteristics of urolithiasis in iraqi children. from 1999 to 2004, 204 children with renal calculi were evaluated. the age range of the patients was 4 months to 14 years, 61.3% of the patients were under 5 years. male to female ratio was 2.8:1. the mean age at onset of symptoms was 3.2 years, and stone disease was diagnosed at a mean of 3.5 years. hematuria (44.6%) and pain (28.4%) were the main clinical presentation. of the 20 ...200516010596
proteus syndrome: an update.proteus syndrome is a complex disorder consisting variably of disproportionate, asymmetric overgrowth of body parts; cerebriform connective tissue nevi; epidermal nevi; vascular malformations of the capillary, venous, and lymphatic types; and dysregulated adipose tissue. serious complications may ensue, such as pulmonary embolism, cystic lung disease, and various neoplasms. somatic mosaicism, lethal in the nonmosaic state, is the best working hypothesis. although proteus syndrome data are consis ...200516010681
[synthesis and antibacterial activity of 3-(5-substituted phenyl-[1,3,4] oxadiazole-2-yl-methylenethio)-5-pyridin-3-yl-[1,2,4] triazole-4-yl-amines].to study on synthesis and antibacterial activity evaluation of polyheterocycles.200516011262
swarmer cell differentiation in proteus mirabilis.under the appropriate environmental conditions, the gram-negative bacterium proteus mirabilis undergoes a remarkable differentiation to form a distinct cell type called a swarmer cell. the swarmer cell is characterized by a 20- to 40-fold increase in both cell length and the number of flagella per cell. environmental conditions required for swarmer cell differentiation include: surface contact, inhibition of flagellar rotation, a sufficient cell density and cell-to-cell signalling. the different ...200516011745
molecular characterization of multidrug-resistant proteus mirabilis isolates from retail meat products.sixty-four multidrug-resistant isolates of proteus mirabilis were collected from retail meat products in oklahoma. the isolates showed four different patterns of antibiotic resistance based on their resistant phenotype and genotypes. most of these isolates were resistant to ampicillin, tetracycline, gentamycin, and kanamycin. class 1 integrons were detected as a common carrier of the antibiotic-resistant genes, such as aada1, aadb, and aada2. a few isolates (9%) contained class 2 integrons with ...200516013378
colouterine fistula mimicking pyometrium--diagnosis established with multi-detector computed tomography. 200516022134
pyrrolobenzodiazepine dimers: novel sequence-selective, dna-interactive, cross-linking agents with activity against gram-positive bacteria.pyrrolo[2,1-c][1,4]benzodiazepine (pbd) dimers are synthetic sequence-selective interstrand dna minor-groove cross-linking agents developed from anthramycins. we investigated the antibacterial activity of three dimers, sjg-136, drg-16 and elb-21, which differ in the structure of the pbd monomeric unit and the length of the linker region between the two identical pbd monomers.200516024592
[species-specific detection of proteus vulgaris and proteus mirabilis by the polymerase chain reaction].sets of primers for the species-specific detection of p. mirabilis and p. vulgaris by the polymerase chain reaction (pcr) were developed. as targets for these primers beta-lactamase and 16s rrna gene fragments were chosen on the basis of the multiple leveling of the sequences of the dna of all known p. mirabilis and p. vulgaris isolates. for differential detection oligonucleotides were selected in such a way that primers, specific for p. vulgaris, contained the non-paired nucleotide for p. mirab ...200516028510
preclinical evaluation of rapeseed, raspberry, and pine bark phenolics for health related effects.rapeseed, raspberry, and pine bark are promising bioactive sources of plant phenolics selected from among ca. 100 previously screened plant materials for in vitro preclinical evaluation of health related effects. phenolic extracts and isolated fractions of the selected materials were investigated for antioxidant, antimicrobial, antiinflammatory, and antimutagenic properties as well as for cell permeability. it was shown that rapeseed and pine bark phenolics and raspberry anthocyanins were good o ...200516028975
extended-spectrum beta-lactamases.the first beta-lactamase was identified in an isolate of escherichia coli in 1940. to date, there are >130 tem-type and >50 sulfhydryl variable (shv)-type beta-lactamases, mainly in e. coli, klebsiella pneumoniae, and proteus mirabilis but also in other members of the enterobacteriaceae family and in some nonenteric organisms, such as acinetobacter species. the incidence of expanded-spectrum beta-lactamases (esbls) varies, depending on which area of the globe the isolates originate from. esbls r ...200516032564
recalcitrant scoliosis in proteus syndrome.proteus syndrome is a rare congenital condition with various clinical features such as hemihypertrophy, macrodactyly, subcutaneous masses, brain-like hyperplasia of the soles and/or palms, epidermal naevi and scoliosis with other mesodermal malformations. multifocal overgrowth can affect various tissues causing severe functional and cosmetic disability, but intellectual and language development are mostly normal. orthopaedic problems include macrodactyly, hindfoot deformity, limb length inequali ...200516035717
antimicrobial susceptibility patterns of contemporary pathogens from uncomplicated urinary tract infections isolated in a multicenter italian survey: possible impact on guidelines.during 2004 four italian laboratories assessed the prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in female out-patients. a total of 600 urine samples from individuals aged 18-65 were studied. the overall prevalence of escherichia coli was 85.3%. klebsiella pneumoniae, staphylococcus saprophyticus, proteus mirabilis, enterococcus faecalis and other rarer species were far less represented. determination of the antibiotic susceptibility pattern of th ...200516038517
antibacterial and antioxidant activities of ethanol extract from paullinia cupana mart.the antibacterial and antioxidant activity of the ethanol extract from paullinia cupana var. sorbilis mart. (sapindaceae) seeds, commonly called guarana, was assessed towards selected bacteria as well as in different antioxidant models. the extract, at a concentration between 16 and 128 microg/ml, showed a significant antibacterial effect expressed as minimum inhibitory concentration (mic) against both gram-negative and gram-positive bacteria. in particular, pseudomonas aeruginosa (mic=16 microg ...200516040216
tigecycline.new antimicrobial agents are urgently needed for clinical use due to the increasing prevalence and spread of multidrug-resistant bacteria that are commonly responsible for serious and life-threatening diseases. the need to develop new agents that effectively overcome existing mechanisms of resistance displayed by bacteria resistant to currently available drugs has become paramount. tigecycline, the first in a new class of antimicrobials, the glycylcyclines, is an analogue of minocycline with add ...200516040625
application of electrospray ionization with fourier transform ion cyclotron resonance mass spectrometry for structural screening of core oligosaccharides from lipopolysaccharides of the bacteria proteus.electrospray ionization with fourier transform ion cyclotron resonance mass spectrometry (esi-fticrms) was used for screening and structural elucidation of core oligosaccharides isolated from lipopolysaccharides of bacteria of the genus proteus. mass spectra allowed the determination of the molecular masses with high accuracy and the estimation of the chemical heterogeneity of the samples. they did not, however, provide sufficient information to identify structural details of the branched oligos ...200516041825
bacterial flora of leg ulcers in patients admitted to department of dermatology, poznań university of medical sciences, during the 1998-2002 period.venous leg ulcers are an important cause of morbidity in a significant percentage of the world population. the percentage of leg ulceration, either active or healed, in the european population is about 1%-2%. the aim of this study was to analyze the rate of colonization and qualitative composition of the bacterial flora isolated from leg ulcers in patients admitted to dermatology department, poznań university of medical sciences, during the 1998-2002 period, with special reference to the infecti ...200516146619
analysis of antibiotic susceptibility and resistance of leg ulcer bacterial flora in patients hospitalized at dermatology department, poznań university hospital.effective treatment of frequently infected, poorly healing wounds such as leg ulcers due to chronic venous insufficiency poses a major clinical problem. antibiotic resistance in dermatology patients, especially those with non-healing leg ulcers, is a widespread phenomenon. various antibiotics, mainly broad-spectrum agents, are frequently and sometimes inappropriately prescribed, which often leads to the selection of antibiotic-resistant bacteria strains. the aim of this study was to analyze anti ...200516146620
synthesis of schiff bases of 2-amino-5-aryl-1,3,4-oxadiazoles and their evaluation for antimicrobial activities.twenty schiff bases of 2-amino-5-aryl-1,3,4-oxadiazoles have been synthesized with different aromatic aldehydes. the structures of the compounds were confirmed by nitrogen analysis, ir and 13c-nmr spectral data. the antibacterial properties of the compounds were investigated against proteus mirabilis (mtcc-425), pseudomonas aeruginosa (mtcc-424), bacillus subtilis (mtcc-619) and staphylococcus aureus (mtcc-96) using the broth dilution method. the most active compounds were 4c (64 microg/ml), 4f ...200515942874
characterisation of the rac/pak pathway in amoeba proteus.molecular mechanisms underlying the unique locomotion of the highly motile amoeba proteus still remain poorly understood. recently, we have shown that blocking the endogenous amoebal rac-like protein(s) leads to distinct and irreversible changes in the appearance of these large migrating cells as well as to a significant inhibition of their locomotion. to elucidate the mechanism of the rac pathway in amoeba proteus, we tested the effects of blocking the endogenous myosin i heavy chain kinase (mi ...200515948264
sesquin, a potent defensin-like antimicrobial peptide from ground beans with inhibitory activities toward tumor cells and hiv-1 reverse transcriptase.an antifungal peptide with a molecular mass around 7 kda and an n-terminal sequence highly homologous to defensin was isolated from ground beans (vigna sesquipedalis cv. 'ground bean'). the peptide was adsorbed on affi-gel blue gel and on mono s. it exerted an antifungal action on botrytis cinerea, fusarium oxysporum and mycosphaerella arachidicola; and an antibacterial action on escherichia coli b, proteus vulgaris, mycobacterium phlei and bacillus megaterium. the antimicrobial activity was inh ...200515949629
adaptation to extreme environments: macromolecular dynamics in complex systems.what we previously thought of as insurmountable physical and chemical barriers to life, we now see as yet another niche harbouring 'extremophiles'. extremophiles and their macromolecules had to develop molecular mechanisms of adaptation to extreme physico-chemical conditions. using neutron spectroscopy, we have demonstrated that molecular dynamics represents one of these molecular mechanisms of adaptation. to which extent do hyper-saline conditions and extreme temperatures influence molecular dy ...200515951115
predictive modelling of growth and measurement of enzymatic synthesis and activity by a cocktail of selected enterobacteriaceae and aeromonas hydrophila.the possibility was examined of developing a predictive model that would predict food spoilage by combining microbial growth (increase in cellular number) with extracellular enzymatic activity of a cocktail of five strains of enterobacteriaceae: escherichia coli, enterobacter agglomerans, klebsiella oxytoca, klebsiella pneumoniae and proteus vulgaris and one aeromonas hydrophila strain. estimations of growth and enzyme activity were made within a three-dimensional matrix of conditions: temperatu ...200516154655
hemihyperplasia with ehlers-danlos syndrome like skin changes.hemihyperplasia is a condition characterized by asymmetric overgrowth of one or more body parts. it may be an isolated finding or may be associated with various syndromes like beckwith-weidemann syndrome, klippel-trenaunay-weber syndrome, proteus syndrome etc. pigmentary skin lesions and capillary malformations are commonly seen in cases of hemihyperplasia. we report a case of hemihyperplasia with associated skin hyperextensibility, varicose veins, skin fragility, easy bruising and papery scars, ...200516155424
extended spectrum beta-lactamase-producing klebsiella pneumoniae chronic ambulatory peritoneal dialysis peritonitis treated successfully with polymyxin b.peritonitis is not an infrequent complication of inpatients with chronic ambulatory peritoneal dialysis (capd). capd peritonitis may be related to the catheter or secondary to perforation of an intra-abdominal viscus. the most common organisms usually associated with capd peritonitis are staphylococcus aureus and staphylococcus epidermidis (coagulase-negative staphylococci). rarely, aerobic gram-negative bacilli have been the causative agents of capd peritonitis. the treatment of capd peritoniti ...200516157192
purple urine bag syndrome in a hemodialysis patient.purple urine bag syndrome (pubs) is an uncommon disorder, in which the plastic disposable urinary catheter bag turns purple or blue following hours or days of urinary catheterization. the purple discoloration results from indirubin dissolved in the plastic mixing with indigo in the urine. bacteria possessing indoxyl sulfatase degrade indoxyl sulfate into indirubin and indigo. indoxyl sulfate is derived from the metabolism of tryptophan. pubs usually occurs in chronic catheterized elderly women w ...200516157987
[efficacy of probiotic bactisporin in therapy of intrahospital urinary infection].we studied efficacy of bactisporin (probiotic based on aerobic spore-forming culture bacillus subtilis, strain 3h) in combined treatment of intrahospital urinary infection (iui) in 36 patients with infravesical obstruction. these patients developed postoperative complications due to iui caused by antibiotic-resistant hospital flora. the control group consisted of 35 patients matched by age and disease given conventional postoperative etiotropic therapy. bacterial translocation enables bactispori ...200516158748
anti-bacterial activity of euphorbia fusiformis--a rare medicinal herb.euphorbia fusiformis buch.-ham. ex. d.don (euphorbiaceae) is a rare medicinal herb. aqueous and organic solvent extracts of the leaves and rootstocks were investigated for anti-bacterial properties by using disc diffusion and well-in agar methods, against pathogenic strains of gram positive (bacillus subtilis and staphylococcus aureus) and gram negative bacteria (escherichia coli, klebsiella pneumoniae, proteus vulgaris, pseudomonas aeruginosa, salmonella typhii a and salmonella typhii b). the d ...200516159702
[surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2003].for the post-marketing surveillance of cefmetazole (cmz, cefmetazon), mics of injectable beta-lactam antibacterials including cmz against clinical isolates from 15 medical institutions all over japan are measured yearly and the incidence rates of resistance in various species are also evaluated. in the first surveillance from june 2000 to march 2001, 574 isolates of 13 species were tested, 548 isolates of the same 13 species were tested in the second surveillance from april 2001 to march 2002, a ...200516161756
regional variation in the prevalence of extended-spectrum beta-lactamase-producing clinical isolates in the asia-pacific region (sentry 1998-2002).we examined the prevalence of extended-spectrum beta-lactamase (esbl)-producing strains of klebsiella pneumoniae, klebsiella oxytoca, escherichia coli, proteus mirabilis, citrobacter koseri, and salmonella spp. that were isolated as part of the sentry asia-pacific surveillance program between 1998 and 2002. during the study period, a total of 6,388 strains were gathered from 17 medical centers in 7 countries and examined for esbl production and hyperproduction of k. oxytoca chromosomal k1 beta-l ...200516165001
structure of a lactic acid ether-containing and glycerol phosphate-containing o-polysaccharide from proteus mirabilis o40.an o-polysaccharide was isolated by mild acid hydrolysis from the lipopolysaccharide of proteus mirabilis o40 and studied by nmr spectroscopy, including 2d 1h, 1h cosy, tocsy, roesy, and 1h, 13c hmqc experiments, along with chemical methods. the polysaccharide was found to contain an ether of glcnac with lactic acid and glycerol phosphate in the main chain and to have the following structure: --> 3)-beta-d-glcpnac4(r-lac)-(1 --> 3)-alpha-d-galp-(1 --> 3)-d-gro-1-p-(o --> 3)-beta-d-glcpnac-(1 --> ...200515890320
outbreaks of contaminated broncho-alveolar lavage related to intrinsically defective bronchoscopes.from 5 march 2001 to 19 october 2001, outbreaks of broncho-alveolar lavage (bal) contamination with enterobacteraceae were detected in our 700-bed institution. we report the investigation of these outbreaks. a case was defined as the occurrence of pairs of specific enterobacteraceae in bal specimens among any patients who underwent bronchoscopy in the respiratory unit during the period of the outbreak. contamination was identified in 117 bal samples during three outbreaks among 418 patients, and ...200515893850
successfully treated mitral valve proteus mirabilis endocarditis.infective endocarditis caused by proteus mirabilis is rare and is fatal in most cases. a case of a 64-year-old man presenting with fever, dysuria, and murmur is reported. p mirabilis endocarditis was diagnosed based on clinical presentation, blood culture findings, and the presence of a large mitral valve vegetation seen on echocardiogram. the patient survived after long-term antibiotics and surgical replacement of the mitral valve. the clinical characteristics and complications from reported ca ...200515894870
vulgarinin, a broad-spectrum antifungal peptide from haricot beans (phaseolus vulgaris).from the seeds of haricot beans (phaseolus vulgaris), an antifungal peptide with a molecular mass around 7 kda was purified by using a simple protocol consisting of affinity chromatography on affi-gel blue gel and gel filtration on superdex 75. this peptide named vulgarinin manifested an antifungal activity toward fungal species such as fusarium oxysporum, mycosphaerella arachidicola, physalospora piricola and botrytis cinerea, and an antibacterial action on mycobacterium phlei, bacillus megater ...200515896669
prulifloxacin versus ciprofloxacin in the treatment of adults with complicated urinary tract infections.the present study was performed to evaluate the efficacy and safety of a 10-day regimen of prulifloxacin 600 mg once daily as compared to ciprofloxacin 500 mg twice daily in the treatment of patients with complicated urinary tract infections (utis).200515897698
enteric bacteria mandibular osteomyelitis.osteomyelitis of the mandible is a relatively rare inflammatory disease that usually stems from the odontogenic polymicrobial flora of the oral cavity. we are reporting 2 unusual cases of mandibular osteomyelitis resulting from enteric bacteria infection. in one patient, abundant clinical evidence suggested a diagnosis of a chronic factitious disease, whereas in the second patient no obvious etiology was found.200515897844
straightforward representation of antimicrobial chemotherapeutics susceptibility profiles in a private tertiary care hospital.culture-based values for antimicrobial susceptibility of 28 antimicrobials against different bacteria isolated from clinical material in a tertiary care hospital were used to pick the most promising antimicrobials. isolates originating from urinary tract, blood and pyogenic foci, enteric fever and enteritis were grouped. antimicrobials, systemic or oral, exhibiting a 75% or more susceptibility were compartmentalized in relation to divergent clinical disorders. retrospective categorized culture-b ...200515904968
characterization of amoeba proteus myosin vi immunoanalog.amoeba proteus, the highly motile free-living unicellular organism, has been widely used as a model to study cell motility. however, molecular mechanisms underlying its unique locomotion and intracellular actin-based-only trafficking remain poorly understood. a search for myosin motors responsible for vesicular transport in these giant cells resulted in detection of 130-kda protein interacting with several polyclonal antibodies against different tail regions of human and chicken myosin vi. this ...200515909304
microbial persistence: a clinical reminder. 200515913172
using ontologies in proteus for modeling proteomics data mining applications.bioinformatics applications are often characterized by a combination of (pre) processing of raw data representing biological elements, (e.g. sequence alignment, structure prediction), and an high level data mining analysis. developing such applications needs knowledge of both data mining and bioinformatics domains, that can be effectively achieved by combining ontology about the application domain and ontology about the approaches and processes to solve the given problem. in this paper we talk a ...200515923712
modeling and designing a proteomics application on proteus.biomedical applications, such as analysis and management of mass spectrometry proteomics experiments, involve heterogeneous platforms and knowledge, massive data sets, and complex algorithms. main requirements of such applications are semantic modeling of the experiments and data analysis, as well as high performance computational platforms. in this paper we propose a software platform allowing to model and execute biomedical applications on the grid.200515924180
characterization of a cdna of peroxiredoxin ii responding to hydrogen peroxide and phagocytosis in amoeba proteus.phagocytic cells have defense systems against reactive oxygen species generated as the first non-specific defense mechanism against invading pathogens or microorganisms. we cloned a cdna encoding a 21.69-kda protein in amoeba proteus homologous to 2-cys peroxiredoxin (prx-ap). in the disk inhibition assay using h2o2 as an oxidizing agent, escherichia coli overproducing prx-ap showed better viability than did e. coli transformed with pbluescript ii sk for control. monoclonal antibodies (mab) prod ...200515926998
sternal split approach to the cervicothoracic junction in children.we present a descriptive case series outlining the surgical technique and outcome in six patients managed with a combined anterior neck and sternal splitting approach.200515928540
preventive and therapeutic administration of an indigenous lactobacillus sp. strain against proteus mirabilis ascending urinary tract infection in a mouse model.probiotics are increasingly being considered as non-pharmaceutical and safe potential alternatives for the treatment and prevention of a variety of pathologies including urinary tract infections. these are the most common infections in medical practice and are frequently treated with antibiotics, which have generated an intense selective pressure over bacterial populations. proteus mirabilis is a common cause of urinary tract infections in catheterised patients and people with abnormalities of t ...200515928974
organisms causing urinary tract infection in pediatric patients at ayub teaching hospital abbottabad.urinary tract infection (uti) is common in pediatric practice and an important cause of morbidity and mortality in children. infected urine stimulates an immunological and inflammatory response leading to renal injury and scarring, ultimately leading to end stage renal failure. the present study identifies the micro organisms causing urinary tract infection in children presenting with uti at ayub teaching hospital, abbottabad.200515929535
plasmid-mediated, inducible ampc beta-lactamase (dha-1)-producing enterobacteriaceae at a korean hospital: wide dissemination in klebsiella pneumoniae and klebsiella oxytoca and emergence in proteus mirabilis.the aim of the study was to investigate the phenotypic and genetic characteristics of recently emerging cefoxitin-resistant and induction-positive isolates of escherichia coli, klebsiella species, and proteus mirabilis. strains of enterobacteriaceae were isolated at a korean tertiary care hospital between june and december 2002. induction was tested using cefoxitin and aztreonam disks, the bladha allele was detected by pcr, and pulsed-field gel electrophoresis (pfge) patterns were also analyzed. ...200515936167
paraparesis caused by an angiolipomatous hamartoma in an adolescent with proteus syndrome and scoliosis.this 16-year-old boy with proteus syndrome suffered acute spinal cord compression secondary to hemorrhage into an extradural paraspinal angiolipomatous hamartoma. a magnetic resonance (mr) imaging study performed 12 years earlier had revealed the presence of this tumor at the apex of a mild thoracic scoliosis, which had been stable during the intervening decade. spinal cord neurological deficit in proteus syndrome is rare. it can arise either from vertebral abnormalities and kyphoscoliotic defor ...200516238085
[effect of the proteolytic enzymes of bacillus licheniformis and the lysoamidase of lysobacter sp. xl1 on proteus vulgaris and proteus mirabilis].preparations of culture liquid of three bacullus licheniformis strains (s, 103, and 60.4) and the enzymatic preparation lysoamidase from culture liquid of lysobacter sp. strain xl1 actively lysed preliminarily autoclaved cells of gram-negative bacteria proteus vulgaris and p. mirabilis. living proteus cells treated with these enzymatic preparations were lysed during their subsequent autoclaving. inoculation of enzyme-treated proteus cells, taken either separately or in combination with one anoth ...200516240656
antibiotic resistance in outpatient urinary isolates: final results from the north american urinary tract infection collaborative alliance (nautica).the goal of the north american urinary tract infection collaborative alliance (nautica) study was to determine antibiotic susceptibility to commonly used agents for urinary tract infections against outpatient urinary isolates obtained in various geographic regions in the usa and canada. forty-one medical centres (30 from the usa and 11 from canada) participated, with each centre submitting up to 50 consecutive outpatient midstream urine isolates. isolates were identified to species level by the ...200516243229
[necrotizing fasciitis of the perineum secondary to a surgical treatment of bartholin's gland abscess].necrotizing fasciitis of the perineum is a rare but of fast evolution, and potentially fatal infectious disease process. it is characterized by progressive inflammation and extensive necrosis of subcutaneous tissue involving the fascia and other adjacent tissues. this infection may be idiopathic or secondary to local trauma or pelvic surgery. its mortality rate is 20%. we report a case of necrotzing fasciitis of the perineum in a 34-year-old woman following incision and drainage of bartholin's g ...200516243570
resolving deep phylogenetic relationships in salamanders: analyses of mitochondrial and nuclear genomic data.phylogenetic relationships among salamander families illustrate analytical challenges inherent to inferring phylogenies in which terminal branches are temporally very long relative to internal branches. we present new mitochondrial dna sequences, approximately 2,100 base pairs from the genes encoding nd1, nd2, coi, and the intervening trna genes for 34 species representing all 10 salamander families, to examine these relationships. parsimony analysis of these mtdna sequences supports monophyly o ...200516243763
surveillance of extended-spectrum beta-lactamases from clinical samples and faecal carriers in barcelona, spain.the aim of the present study was to characterize and compare the extended-spectrum beta-lactamase (esbl)-producing organisms isolated from clinical samples and faecal carriers in 2001 and 2002.200516244084
the ability of proteus mirabilis to sense surfaces and regulate virulence gene expression involves flil, a flagellar basal body protein.proteus mirabilis is a urinary tract pathogen that differentiates from a short swimmer cell to an elongated, highly flagellated swarmer cell. swarmer cell differentiation parallels an increased expression of several virulence factors, suggesting that both processes are controlled by the same signal. the molecular nature of this signal is not known but is hypothesized to involve the inhibition of flagellar rotation. in this study, data are presented supporting the idea that conditions inhibiting ...200516166542
phylogeny of the enterobacteriaceae based on genes encoding elongation factor tu and f-atpase beta-subunit.the phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor tu gene (tuf) and of their f-atpase beta-subunit gene (atpd). an 884 bp fragment for tuf and an 884 or 871 bp fragment for atpd were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. the atpd sequence analysis exhibited an indel specific to pantoea and tatumella species, showing, for the first time, a tight phylogenetic ...200516166704
genotyping of urinary and fecal proteus mirabilis isolates from individuals with long-term urinary catheters. 200516167137
influence of human ascitic fluid on the in vitro antibacterial activity of moxifloxacin.we investigated the in vitro influence of haf on the antibacterial activity of moxifloxacin against escherichia coli atcc 10798, escherichia coli k-12, proteus rettgeri (sanelli), staphylococcus aureus atcc 25923, staphylococcus aureus nctc 1808 and staphylococcus epidermidis atcc 12228. human ascitic fluid was obtained from 6 cirrhotic patients by paracentesis. the interaction effect was evaluated by the checkerboard technique. our results indicate the ability of human ascitic fluid to reduce m ...200516167519
bacteriology of surgical wound infections in a tertiary care hospital in turkey.to determine the spectrum of the pathogens cultured from surgical wound infections and assess their antimicrobial drug resistances.200516167704
in vitro and in vivo antibacterial activities of sm-216601, a new broad-spectrum parenteral carbapenem.sm-216601 is a novel parenteral 1beta-methylcarbapenem. in agar dilution susceptibility testing, the mic of sm-216601 for 90% of the methicillin-resistant staphylococcus aureus (mrsa) strains tested (mic(90)) was 2 microg/ml, which was comparable to those of vancomycin and linezolid. sm-216601 was also very potent against enterococcus faecium, including vancomycin-resistant strains (mic(90) = 8 microg/ml). sm-216601 exhibited potent activity against penicillin-resistant streptococcus pneumoniae, ...200516189097
granulomatous inflammation of salt glands in ducklings (anas platyrhynchos) associated with intralesional gram-negative bacteria.the "nasal glands" occur in many bird species and are powerful sodium ion-excretory organs. in ducks, they are located in supraorbital bony recesses. granulomatous inflammation of these glands occurs with an incidence of approximately 1% in ducklings (anas platyrhynchos), and is not associated with specific clinical symptoms. we investigated nine glands of eight animals with granulomas by gross pathology and histopathology, and compared results of bacteriology with 20 non-lesioned nasal glands. ...200516191707
identification and antimicrobial susceptibility of micro-organisms recovered from cutaneous lesions of human american tegumentary leishmaniasis in minas gerais, brazil.an evaluation of the microbiota present in cutaneous ulcers from 31 patients with a clinical and parasitological diagnosis of american tegumentary leishmaniasis (atl) was carried out by the standard filter paper disc technique, including antimicrobial susceptibility of the bacterial isolates. microbial examination indicated that 21 patients (67.7%) were contaminated with one to four bacteria and some of them also with yeast. a total of 142 micro-organisms were isolated. staphylococcus aureus was ...200516192439
multiple origins and incursions of the atlantic barnacle chthamalus proteus in the pacific.chthamalus proteus, a barnacle native to the caribbean and western atlantic, was introduced to the pacific within the last few decades. using direct sequencing of mitochondrial dna (coi), we characterized genetic variation in native and introduced populations and searched for genetic matches between regions to determine if there were multiple geographical sources and introduction points for this barnacle. in the native range, we found great genetic differences among populations (max. f(st) = 0.6 ...200516202091
in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes.a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ...200516206827
n,n-dichlorotaurine: chemical and bactericidal properties.the biogenous antimicrobial agent n-chlorotaurine (nct) converts by disproportionation to n,n-dichlorotaurine (ndct) at a rate proportional to acidity. this occurs at appreciable amounts already in weakly acidic biological systems. to understand the consequences of ndct formation, a thorough investigation of this undescribed compound was mandatory, which needed its synthesis. differently from nct, this was possible in the aqueous system using trichloroisocyanuric acid. while the free acid, cl(2) ...200516211659
increase of faecal bifidobacteria due to dietary oligosaccharides induces a reduction of clinically relevant pathogen germs in the faeces of formula-fed preterm infants.in a previous study on formula-fed preterm infants, we were able to demonstrate that dietary oligosaccharides (a mixture of 90% galacto-oligosaccharides and 10% fructo-oligosaccharides in a concentration of 1 g/dl) stimulate the growth of faecal bifidobacteria. in the present explorative analysis of this study, we focus on the effect of the dominance of bifidobacteria on the presence of clinically relevant pathogens (staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, ...200516214763
[prevalence and risk factors for bacteriuria in patients with cirrhosis].bacterial infections occur frequently in patients with cirrhosis and may worsen the disease outcome. we investigated the prevalence of bacteriuria in 500 consecutive patients with cirrhosis, in different child-pugh stages (41.4% a; 40.8% b; 17% c) and analysed the associated risk factors. most of the cirrhosis cases were virus related; alcohol abuse was recorded in 6.2% of the patients. bacteriuria was detected in 139 (27.8%) cases: 32.4% were more than 100,000 cfu/ml; 7.9% between 100,000 and 1 ...200516220030
plasma and urinary adrenomedullin levels in children with acute pyelonephritis.adrenomedullin (am), a novel peptide recently isolated from pheochromocytoma, eliciting vasorelaxing activity, is the strongest among all known peptides. am has been detected in the adrenal medulla, cardiac tissue, lung and kidney. immunohistochemical studies have demonstrated the localization of am in glomeruli, tubules and collecting cells of the kidney. clinically, plasma and urinary am levels are altered in patients with different renal disease. the present study aims to determine plasma and ...200516221100
extended spectrum beta-lactamases among gram-negative bacteria of nosocomial origin from an intensive care unit of a tertiary health facility in tanzania.resistance to third generation cephalosporins due to acquisition and expression of extended spectrum beta-lactamase (esbl) enzymes among gram-negative bacteria is on the increase. presence of esbl producing organisms has been reported to significantly affect the course and outcome of an infection. therefore infections due to esbl isolates continue to pose a challenge to infection management worldwide. the aim of this study was to determine the existence and to describe phenotypic and genotypic c ...200516225701
[dercum's disease: a case report].a case of dercum's disease in a 51-year-old obese woman with a history of brain vasculitis, and painful subcutaneous multiple lipomas is described. this disease, included in the category of rare diseases by the world health organization, first described in 1892 by francis xavier dercum, is characterized by its prevalence among women, its familiarity, by the presence of multiple painful subcutaneous lipomas and its association with obesity, hypercholesterolemia and asthenia. the disease has to be ...200516250185
characterization and biological activities of two copper(ii) complexes with diethylenetriamine and 2,2'-bipyridine or 1,10-phenanthroline as ligands.two new mixed ligand copper(ii) complexes with diethylenetriamine, 2,2'-bipyridine and 1,10-phenanthroline have been synthesized. the crystal and molecular structures of [cu(dien)(phen)](clo(4))(2) and [cu(dien)(bipy)](bf(4))(2) (dien=diethylenetriamine, phen=1,10-phenanthroline, bipy=2,2'-bipyridine) were determined by x-ray crystallography from single crystal data. these two complexes have similar structures. the epr spectral data also suggest that these complexes have distorted square pyramid ...200516257723
outcome of bacterial culture from mastoid granulations: is it relevant in chronic ear disease?to detect the presence of bacteria in mastoid granulations and compare its prevalence in both types of chronic suppurative otitis media (csom). to find out if stage of disease activity, age, duration of disease, and aditus patency relate to obtaining positive cultures.200516259653
lunatusin, a trypsin-stable antimicrobial peptide from lima beans (phaseolus lunatus l.).an anti-fungal peptide designated as lunatusin, with a molecular mass around 7kda, was purified from the seeds of chinese lima bean (phaseolus lunatus l.). the peptide was isolated using a simple protocol consisting of affinity chromatography on affi-gel blue gel and gel filtration on superdex 75. lunatusin exerted an anti-fungal activity toward fungal species such as fusarium oxysporum, mycosphaerella arachidicola and botrytis cinerea, and an antibacterial action on, bacillus megaterium, bacill ...200516269344
relationships between antimicrobial use and antimicrobial resistance in gram-negative bacteria causing nosocomial infections from 1991-2003 at a university hospital in taiwan.this study was conducted to evaluate the relationship between antimicrobial resistance and antimicrobial use in a university hospital in taiwan. disk susceptibility data of escherichia coli, klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, proteus spp., pseudomonas aeruginosa, acinetobacter spp., stenotrophomonas maltophilia and other non-fermentative gram-negative bacilli causing nosocomial infections were evaluated. data on annual patient-days and annual consumption (defined d ...200516280243
structures of the o-polysaccharides and classification of proteus genomospecies 4, 5 and 6 into respective proteus serogroups.an acidic branched o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (lps) of proteus genomospecies 4 and studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, roesy and h-detected 1h, 13c hsqc experiments. the following structure of the pentasaccharide repeating unit of the o-polysaccharide was established, which is unique among proteus polysaccharide structures: [structure: see text] where qui3nac stands for 3 ...200516262693
bacterial flora of the low male genital tract in patients consulting for infertility.the physiological aerobic bacterial flora of the low male genital tract was determined. this prospective study was performed on 600 semen specimens collected from 543 asymptomatic males consulting for infertility. semen cultures were sterile in 28.8%, with a polymicrobial flora and/or absence or low titres of ureaplasma urealyticum in 49.3%, and with one or two aerobic and facultative bacteria > or =1 x 10(3) cfu ml(-1) and/or u. urealyticum with titres > or =10(4) ccu ml(-1) (colour changing un ...200516266393
microbiology of dacryocystitis among adults population in southern australia.retrospective study on microbiology of dacryocystitis cases in adults was carried out at the royal victorian eye and ear hospital (rveeh). sixty-four consecutive cases seen between march 2000 and june 2003 were included. seventy-nine organisms were isolated from the sixty-six samples (1.2/specimen). aerobic or facultative bacteria were recovered in 76 (96.2), fungi from two specimens (2.5%). and anaerobic bacteria from only one specimen (1.3%). staphylococcus aureus (27), was most common followe ...200516295714
particularities of bacterial ecology of acute otitis media in an african subtropical country (cote d'ivoire).acute otitis media (aom) is the commonest infectious disease of childhood. its treatment is based on a probabilistic antibiotic therapy.200616297453
the incidence of proteus mirabilis infection increases in patients on treatment but does not trigger disease activity.the aim of this study is to determine whether treatment increases the levels of anti-proteus antibodies (apa) in patients with rheumatoid arthritis (ra). the blood samples of 32 patients suffering from ra who were recruited in our previous study and continued to participate in our follow-up study were collected after 1 year. their first and follow-up samples were analysed for the presence of igg isotype and total immunoglobulins (igg+iga+igm) against proteus mirabalis (pm) using enzyme-linked im ...200616267598
a structured-population model of proteus mirabilis swarm-colony development.in this paper we present continuous age- and space-structured models and numerical computations of proteus mirabilis swarm-colony development. we base the mathematical representation of the cell-cycle dynamics of proteus mirabilis on those developed by esipov and shapiro, which are the best understood aspects of the system, and we make minimum assumptions about less-understood mechanisms, such as precise forms of the spatial diffusion. the models in this paper have explicit age-structure and, wh ...200616283413
occurrence of extended-spectrum (beta)-lactamases and plasmid-mediated ampc (beta)-lactamases among korean isolates of proteus mirabilis. 200616284101
identification and phylogenetic analysis of arsenophonus- and photorhabdus-type bacteria from adult hippoboscidae and streblidae (hippoboscoidea).this is the first report of arsenophonus- and photorhabdus-type bacteria from streblidae (bat flies) and hippoboscidae (louse flies, keds). strains were detected by means of polymerase chain reaction of 16s rdna, and phylogenetic analysis determined the relationship of the obtained sequences to previously reported sequences in genbank. phylogenetic analysis by means of maximum parsimony revealed that all isolated arsenophonus spp. 16s rdna sequences formed a monophyletic sub-clade within other i ...200616289111
dna sequence analysis of the genetic environment of various blactx-m genes.over a 3 year period (2000-2003) 21 escherichia coli, 5 klebsiella pneumoniae, 1 serratia marcescens and 1 proteus mirabilis producing ctx-m-type beta-lactamase were collected from five different hospitals in paris, france. this study was conducted to analyse the genetic environment of these 28 bla(ctx-m) genes.200616291869
synthesis and evaluation of new quinazolone derivatives of nalidixic acid as potential antibacterial and antifungal agents.in continuation of our work on synthesis of biheterocycles carrying the biodynamic heterocyclic systems at position 3, a series of new nalidixic acid derivatives having quinazolones moiety were synthesised to achieve enhanced biological activity and wide spectrum of activity. nalidixic acid was first converted into its acid chloride using thionyl chloride as an acylating agent at laboratory temperature. later it was converted to methyl ester. nalidixoyl chloride formed vigorously reacts with met ...200616260068
antibacterial and wound healing properties of methanolic extracts of some nigerian medicinal plants.this study was conducted to evaluate methanolic extracts of ageratum conyzoides, anthocleista djalonensis, napoleona imperialis, ocimum gratissimum, and psidium guajava for antibacterial and wound healing properties. antibacterial properties of the extracts were studied against eleven wound isolates (staphylococcus aureus (four strains), e. coli (two strains), pseudomonas aeruginosa (one strain), proteus spp. (three strains), and shigella spp. (one strain)) using the well diffusion method. wound ...200616226414
metabolic activation of bladder procarcinogens, 2-aminofluorene, 4-aminobiphenyl, and benzidine by pseudomonas aeruginosa and other human endogenous bacteria.pseudomonas aeruginosa, an opportunistic pathogen of the human urinary tract, and other selected human endogenous bacteria were investigated for metabolic activation of the bladder procarcinogens, 2-aminofluorene (2-af), 4-aminobiphenyl (4-ab), and benzidine (bz). the cell-free extracts of pseudomonas aeruginosa, escherichia coli, enterobacter aerogenes, proteus mirabilis, proteus vulgaris, staphylococcus epidermidis, staphylococcus saprophyticus, klebsiella pneumoniae, and intestinal anaerobes, ...200616203120
evolutionarily conserved structural motifs in bacterial gst (glutathione s-transferase) are involved in protein folding and stability.the bacterium proteus mirabilis expresses a cytosolic class beta glutathione s-transferase (pmgst b1-1) that is part of a family of multifunctional detoxication enzymes. like other cytosolic gsts, pmgst b1-1 possesses two local structural motifs, an n-capping box and a hydrophobic staple motif, both of which are located between amino acids 151 and 156. the n-capping box consists of a reciprocal hydrogen bonding interaction of thr152 with asp155, whereas the hydrophobic staple motif consists of a ...200616248855
toxicological effect of indole and its azo dye derivatives on some microorganisms under aerobic conditions.azo dyes are ubiquitous commercial chemicals that present unique environmental problems. the azo dyes in particular can undergo natural anaerobic degradation to potentially carcinogenic amines. they pose a major problem for water-treatment plants downstream. one strategy to remidate polluted surface contaminants is to make use of the degradative capacity of bacteria rather than using destructive chemical reactions. therefore, pathogenic and non-pathogenic microorganisms namely; bacillus thuringi ...200616165191
[patterns of susceptibility to antibiotics of enterobacteriaceae causing intra-abdominal infection in spain: smart 2003 study outcomes].smart (study for monitoring antimicrobial resistance trends) is an ongoing global antimicrobial surveillance program focused on clinical isolates from intra-abdominal infections. the objective of this subanalysis was to assess antimicrobial susceptibility patterns among entero-bacteriaceae recovered at 13 participating spanish sites during 2003. antimicrobial susceptibility testing was performed using broth microdilution techniques according to the clsi (formerly nccls) guidelines for mic testin ...200616688292
antibacterial and antioxidant activities in sideritis italica (miller) greuter et burdet essential oils.sideritis italica (miller) greuter et burdet is a widespread lamiacea in the mediterranean region used in traditional medicine. essential oils were antibacterial against nine atcc and as many clinically isolated gram-positive and gram-negative bacterial strains. antibacterial activity was also found against helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. the antibacterial activity of the oils was expressed as mics (minimum inhibitory concentrations) and mbc ...200616690235
otogenic cerebellar abscess: an unusual occurrence. 200616696351
triclosan loaded ureteral stents decrease proteus mirabilis 296 infection in a rabbit urinary tract infection model.infection and encrustation remain major limitations of ureteral stent use and to our knowledge no device has completely overcome these obstacles to date. triclosan is a biocide currently used in a plethora of consumer and medical products that has recently been loaded into a ureteral stent. using a rabbit model of uti we examined the effects of triclosan impregnated stent segments on the growth and survival of proteus mirabilis, a uropathogen commonly associated with device related uti and encru ...200616697868
structure of the o-polysaccharide of proteus mirabilis ccug 10705 (of) containing an amide of d-galacturonic acid with l-alanine.the structure of the o-polysaccharide of proteus mirabilis ccug 10705 (of) was determined by chemical analyses along with one- and two-dimensional (1)h and (13)c nmr spectroscopy. the polysaccharide was found to contain an amide of d-galacturonic acid with l-alanine and based on the uniqueness of the o-polysaccharide structure and serological data, it was suggested to classify p. mirabilis of into a new separate proteus serogroup, o74. a weak cross-reactivity of p. mirabilis of and p. mirabilis ...200616697996
gram-negative meningitis and infections in individuals treated with intrathecal baclofen for spasticity: a retrospective study.the aim of this retrospective study was to describe signs, symptoms, and clinical outcomes of individuals undergoing intrathecal baclofen (itb) therapy who experienced pump-related gram-negative infections including meningitis. participants included 12 individuals (nine males, three females) aged 10 to 32 years (mean 17y 9mo), nine of whom had quadriplegic cp. a total of 571 baclofen pump surgeries were performed with 45 total infections. of the 45 infections, 12 were by gram-negative organisms, ...200616700935
epidermal naevus in proteus syndrome showing loss of heterozygosity for an inherited pten mutation.a 3-year-old boy with proteus syndrome has a novel germline p.y68d mutation of the pten gene inherited from his mother who has cowden syndrome. in addition, dna extracted from curettings of his widespread epidermal naevus shows loss of heterozygosity for this mutation. to our knowledge, this has not been described before.200616704655
the anti-microbial activity of electrolysed oxidizing water against microorganisms relevant in veterinary medicine.standards of the german association of veterinary medicine (dvg) for the evaluation of chemical disinfectants were used to assess the anti-microbial efficacy of electrolysed oxidizing water (eow). enterococcus faecium, mycobacterium avium subspecies avium, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus and candida albicans were exposed to anode eow (ph, 3.0+/-0.1; oxidation-reduction potential (orp), +1100+/-50 mv; free chlorine, 400+/-20 mg/l cl2) and combined eow (7:3 anode:c ...200616629725
observations on the adherence of proteus mirabilis onto polymer surfaces.infection of the catheterized urinary tract with proteus mirabilis causes blockage of the catheter by crystalline bacterial biofilms. the aim of this work is to identify a surface-coating for catheters that is not vulnerable to colonization by pr. mirabilis.200616630003
lipid-specific membrane activity of human beta-defensin-3.defensins represent a major component of innate host defense against bacteria, fungi, and enveloped viruses. one potent defensin found, e.g., in epithelia, is the polycationic human beta-defensin-3 (hbd3). we investigated the role of the lipid matrix composition, and in particular the presence of negatively charged lipopolysaccharides (lps) from sensitive (escherichia coli, salmonella enterica serovar minnesota) or resistant (proteus mirabilis) gram-negative bacteria or of the zwitterionic phosp ...200616634647
[sensitivity and resistance of aerobic bacteria isolated from patients with periodontitis towards antibiotics and bacteriophages (comparative analysis)].in order to examine sensitivity and resistance of isolated aerobic bacteria from periodontitis materials towards antibiotics and bacteriophages, there has been studied exudations taken from 737 patients' periodontic pockets or the tissue taken from curettage. according to the rate of identified microorganisms, they have been arranged as follows: s. epidermidis 39,34+/-1,56%; s. pyogenes 18,84+/-1,25%; m. catarrhalis 17,09+/-1,2%; s. aureus 10,71+/-0,99%; e.coli-5,66+/-0,74%; diphtheroids in 1,13 ...200616636376
pathogenic bacteria carried by companion animals and their susceptibility to antibacterial agents.results of the investigation showed that there was a difference in the bacteria isolated from dogs, cats and their living environment. the number and species isolated from the hair and front paw samples from dogs kept outdoors and from cats were greater and more varied than those from the samples from dogs kept indoors. staphylococcus, micrococcus and bacillus were frequently detected from skin surfaces. on the other hand, escherichia, pseudomonas, proteus and others were detected on each sampli ...200616637433
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