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therapeutic potential of gramicidin s in the treatment of root canal infections.an intrinsic clindamycin-resistant enterococcus faecalis, the most common single species present in teeth after failed root canal therapy, often possesses acquired tetracycline resistance. in these cases, root canal infections are commonly treated with ledermix(®) paste, which contains demeclocycline, or the new alternative endodontic paste odontopaste, which contains clindamycin; however, these treatments are often ineffective. we studied the killing activity of the cyclic antimicrobial peptide ...201627618065
superior antibacterial activity of glcn-aunp-go by ultraviolet irradiation.a complete bacterialysis analysis of glucosamine-gold nanoparticle-graphene oxide (glcn-aunp-go) and uv-irradiated glcn-aunp-go was conducted. analytical characterization of glcn-aunps, go and glcn-aunp-go revealed uv-vis absorbance peak at around 230 and 500nm. microscopic characterization of prepared nanomaterials was performed by scanning electron microscope, atomic force microscopy, and high-resolution transmission microscopy. the results confirmed that the glcn-aunps were uniformly decorate ...201627612724
longitudinal (2001-14) analysis of enterococci and vre causing invasive infections in european and us hospitals, including a contemporary (2010-13) analysis of oritavancin in vitro potency.the objective of this study was to evaluate the prevalence and in vitro susceptibility of enterococci and vre among bloodstream infections in european and us hospitals over time.201627609052
in vitro activities of tedizolid compared with other antibiotics against gram-positive pathogens associated with hospital-acquired pneumonia, skin and soft tissue infection and bloodstream infection collected from 26 hospitals in china.to evaluate the in vitro antimicrobial activities of tedizolid, linezolid and other comparators against clinically significant gram-positive cocci isolates from hospital-acquired pneumonia (hap), skin and soft tissue infection (ssti) and bloodstream infection (bsi), 2140 nonduplicate isolates (23.7 % isolated from hap, 46.8 % from ssti and 29.5 % from bsi) were consecutively collected in 26 hospitals in 17 cities across china during 2014. these pathogens included 632 methicillin-resistant staphy ...201627599998
high-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays.methods to rapidly assess cell growth would be useful for many applications, including drug susceptibility testing, but current technologies have limited sensitivity or throughput. here we present an approach to precisely and rapidly measure growth rates of many individual cells simultaneously. we flow cells in suspension through a microfluidic channel with 10-12 resonant mass sensors distributed along its length, weighing each cell repeatedly over the 4-20 min it spends in the channel. because ...201627598230
[antibacterial and residual antimicrobial activities of five final irrigants in infected root canal: an in vitro comparative study].to evaluate antibacterial and residual antimicrobial activities of five root canal irrigants including qmix, mtad(mixture of a tetracycline isomer, an acid, and a detergent), 0.2% cetrimide(ctr), 2% chlorhexidine(chx) and 17% ethylene diaminetetraacetic acid(edta) and to find the most optimal final irrigants for using in root canal therapy.201627596342
effect of fiber insertion depth on antibacterial efficacy of photodynamic therapy against enterococcus faecalis in rootcanals.this in vitro study evaluated the effect of fiber insertion depth on antimicrobial efficacy of antimicrobial photodynamic therapy (apdt) using a photosensitizer (ps; toluidine blue) and a red light-emitting diode (led) in root canals infected with enterococcus faecalis.201627591860
antimicrobial and antibiofilm activity of baccharis psiadioides essential oil against antibiotic-resistant enterococcus faecalis strains.baccharis psiadioides (less.) joch. müller (asteraceae) is considered as a producer of bioactive essential oils and is used in south brazilian folk medicine for its proprieties as stimulant, antipyretic, anti-inflammatory and as an antidote for snake bites.201627590861
microbicidal effects of plain soap vs triclocarban-based antibacterial soap.the aim of this study was to determine the bactericidal effects of plain and antibacterial soap. the bactericidal effects of plain and antibacterial soap containing 0.3% triclocarban were examined against 10 gram-positive and 10 gram-negative bacterial strains after exposure at 22°c and 40°c for 20 s. gram-negative bacteria were more susceptible to both soaps than gram-positive bacteria. however, with one exception (enterococcus faecalis atcc 19433 at 40°c), there was no significant difference b ...201627585555
effect of different mixing methods on the bacterial microleakage of calcium-enriched mixture cement.calcium-enriched mixture (cem) cement is used in the field of endodontics. it is similar to mineral trioxide aggregate in its main ingredients. the present study investigated the effect of different mixing methods on the bacterial microleakage of cem cement.201627580651
molecular basis for lytic bacteriophage resistance in enterococci.the human intestine harbors diverse communities of bacteria and bacteriophages. given the specificity of phages for their bacterial hosts, there is growing interest in using phage therapies to combat the rising incidence of multidrug-resistant bacterial infections. a significant barrier to such therapies is the rapid development of phage-resistant bacteria, highlighting the need to understand how bacteria acquire phage resistance in vivo here we identify novel lytic phages in municipal raw sewag ...201627578757
genome-based characterization of hospital-adapted enterococcus faecalis lineages.vancomycin-resistant enterococcus faecalis (vrefs) is an important nosocomial pathogen(1,2). we undertook whole genome sequencing of e. faecalis associated with bloodstream infection in the uk and ireland over more than a decade to determine the population structure and genetic associations with hospital adaptation. three lineages predominated in the population, two of which (l1 and l2) were nationally distributed, and one (l3) geographically restricted. genome comparison with a global collectio ...201627572164
microbiological evaluation of the extension wire and percutaneous epidural lead anchor site following a "2-stage cut-down" spinal cord stimulator procedure.there are concerns regarding bacterial colonization of the temporary extension leads and subsequent infection risk using the 2-stage cut-down approach in spinal cord stimulation (scs). we sought to quantify the extent of bacterial colonization of the temporary extension wire and percutaneous epidural lead anchor site.201627910210
comparison of antibacterial efficacy of turmeric extract, morinda citrifolia and 3% sodium hypochlorite on enterococcus faecalis: an in-vitro study.sodium hypochlorite (naocl), the most commonly used irrigant, has many potential properties like its unique ability to dissolve pulp tissue, excellent antimicrobial activity, but has a cytotoxic effect when injected into periapical tissues. it is also known to produce allergic reactions, foul smell and taste, and potential for corrosion. facultative organisms such as enterococcus faecalis and aerobes like staphylococcus aureus are considered to be the most resistant species and one of the possib ...201627891459
comparison of the most common isolates of postoperative endophthalmitis in south korea; enterococcus species vs coagulase-negative staphylococci.to compare the related factors or manifestations of the two most common isolates of post-operative endophthalmitis, which were enterococcus spp. and coagulase-negative staphylococci (cns) in south korea.201627887598
bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide against enterococcus faecalis.the aim of the present study was to assess the antimicrobial activity of plasma jet with helium (he) flowing through 3% hydrogen peroxide in root canals infected with enterococcus faecalis. a total of 42 single-rooted anterior teeth were prepared, sterilized, inoculated with an e. faecalis suspension and incubated for 7 days. next, the teeth were randomly divided into six experimental groups (including groups treated by plasma jet with or without he for different time durations) and one control ...201627882119
treatment of oral biofilms by a d-enantiomeric peptide.almost all dental diseases are caused by biofilms that consist of multispecies communities. djk-5, which is a short d-enantiomeric, protease-resistant peptide with broad-spectrum anti-biofilm activity, was tested for its effect on oral multispecies biofilms. peptide djk-5 at 10 μg/ml effectively prevented the growth of these microbes in culture media in a time-dependent manner. in addition to the prevention of growth, peptide djk-5 completely killed both streptococcus mutans and enterococcus fae ...201627880799
intrathecal/intraventricular linezolid in multidrug-resistant enterococcus faecalis ventriculitis.background the use of intrathecal antibiotic therapy for the treatment of ventriculitis and/or meningitis has demonstrated efficacy especially when sterilization of the cerebrospinal fluid is not possible with intravenous antibiotics alone. case description we describe the successful treatment of enterococcus faecalis ventriculitis utilizing intrathecal linezolid in a 32-year-old female patient with severe allergy to vancomycin, prohibitive bacterial susceptibilities, and failure of previous att ...201627867829
identification of chemical compounds that inhibit protein synthesis in pseudomonas aeruginosa.four inhibitory compounds were identified using a poly-uridylic acid (polyu) mrna-directed aminoacylation/translation (a/t) protein synthesis system composed of phenylalanyl-trna synthetases (phers), ribosomes, and ribosomal factors from pseudomonas aeruginosa in an in vitro screen of a synthetic compound library. the compounds were specific for inhibition of bacterial protein synthesis. in enzymatic assays, the compounds inhibited protein synthesis with ic50 values ranging from 20 to 60 μm. min ...201627872201
co-evolutionary dynamics between a defensive microbe and a pathogen driven by fluctuating selection.microbes that protect their hosts from pathogenic infection are widespread components of the microbiota of both plants and animals. it has been found that interactions between 'defensive' microbes and pathogens can be genotype-specific and even underlie the variation in host resistance to pathogenic infection. these observations suggest a dynamic co-evolutionary association between pathogens and defensive microbes, but direct evidence of co-evolution is lacking. we tested the hypothesis that def ...201627862515
comparative assessment of antimicrobial efficacy of different hand sanitizers: an in vitro study.to evaluate the antimicrobial efficacy of four different hand sanitizers against staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, and enterococcus faecalis as well as to assess and compare the antimicrobial effectiveness among four different hand sanitizers.201627857768
pooled analysis of single-dose oritavancin in the treatment of acute bacterial skin and skin-structure infections caused by gram-positive pathogens, including a large patient subset with methicillin-resistant staphylococcus aureus.oritavancin is a lipoglycopeptide antibiotic with bactericidal activity against gram-positive pathogens, including methicillin-resistant staphylococcus aureus (mrsa). the phase 3 studies solo i and solo ii demonstrated comparable efficacy and safety of a single dose of oritavancin compared with 7-10 days of twice-daily vancomycin in adults with acute bacterial skin and skin-structure infections (absssis). the present analysis assessed clinical responses by pathogen at 48-72 h and at study days 1 ...201627665522
evaluation of the ability of different detergents and disinfectants to remove and kill organisms in traditional biofilm.the objective of this study was to assess the ability of different detergent and disinfectant combinations to eradicate bacteria in traditional biofilm.201627234012
comparative analysis of saf, protaper next and bt-race in eliminating enterococcus faecalis from long oval canals: an ex vivo study.comparison of the ability of newly designed rotary files to eliminate viable enterococcus faecalis populations from long oval root canals of extracted human teeth to that of the self-adjusting file (saf). one hundred caries-free, single-rooted, long oval teeth were contaminated with e. faecalis. the teeth were randomly distributed into four groups (n = 25) as follows: g.1, manual; g.2, saf; g.3, protaper next; and g.4, bt-race. two microbial samples were obtained from each tooth with sterile pap ...201627808450
glutathione s-transferase alpha 4 induction by activator protein 1 in colorectal cancer.glutathione s-transferase alpha 4 (gsta4) is a phase ii detoxifying enzyme that metabolizes electrophiles and carcinogens including 4-hydroxy-2-nonenal (4-hne), an endogenous carcinogen that contributes to colorectal carcinogenesis. in this study, we investigated gsta4 expression and regulation in murine primary colonic epithelial cells, microbiome-driven murine colitis and human carcinomas. exposure of yamc cells to 4-hne induced gsta4 expression. using an inflammation-associated model of color ...201627065323
harnessing bacteriocin biology as targeted therapy in the gi tract.recently, our laboratory demonstrated that bacteriocins produced by commensal enterococci provide an advantage in niche maintenance in the highly competitive environment of the gastrointestinal (gi) tract. bacterial production of bacteriocins is a conserved defense strategy to help establish an ecological niche. bacteriocin-encoding genes in enterococci are often carried on mobile genetic elements, including conjugative plasmids, enabling the transfer of such traits to other community members in ...201627624536
vertebral osteomyelitis associated with single and mixed bacterial infection in broilers.vertebral osteomyelitis (vo) is a worldwide emerging disease that affects broilers. the objective of this study was to determine the frequency and aetiology of vo in broilers in a highly productive broiler region. for this, 608 broilers with locomotory problems were analysed from 18 farms. clinical signs were recorded, necropsy was performed and samples were collected from vertebral bodies with gross changes for molecular and histopathological analysis and for bacterial isolation. from broilers ...201627315453
antibacterial efficacy of super-oxidized water on enterococcus faecalis biofilms in root canal.the success of endodontic treatment depends on a few crucial factors. one of these factors is the complete chemomechanic preparation of root canal against various bacteria. in particular, the effect of resistant bacteria may cause intense pain with flare-up and formation of periapical lesions. therefore, the strong effect of irrigants plays an important role in terms of the complete elimination of these bacteria to achieve long-term successful treatment.201627800142
endocarditis and monocular blindness.a man aged 77 years with postrenal transplant lymphoproliferative disease was admitted with high fever, elevated inflammatory markers and a heart murmur. blood cultures grew enterococcus faecalis and he was found to have mitral valve endocarditis on echocardiogram and subsequently started on appropriate antibiotics. 5 days into treatment, he developed ocular symptoms and 3 days later, he had irreversible monocular visual loss. he was seen by the ophthalmology team who diagnosed endogenous endopt ...201627797843
draft genome sequence of enterococcus faecalis strain f165 isolated from a urinary tract infection.we report here a draft genome sequence of enterococcus faecalis strain f165 isolated from a urine specimen in south brazil. the genome size was 3,049,734 bp, with a g+c content of 37.38%, and genes related to antimicrobial resistance and adherence were found in the strain. these findings are consistent with pathogenesis of e. faecalis species.201627795252
antibacterial efficacy of neem (azadirachta indica) extract against enterococcus faecalis: an in vitro study.debridement and disinfection of the root canal is a crucial step in the success of endodontic treatment. several antimicrobial agents alone or in combination are used to achieve this. the objective of this in vitro study was to assess the antimicrobial efficacy of neem (azadirachta indica) extract against enterococcus faecalis.201627794147
characterization of antimicrobial resistance and quinolone resistance factors in high-level ciprofloxacin-resistant enterococcus faecalis and enterococcus faecium isolates obtained from fresh produce and fecal samples of patients.the emergence of fluoroquinolone-resistant enterococci is worldwide. antimicrobial resistance was characterized and the effect of quinolone-resistance factors was analyzed in high-level ciprofloxacin-resistant (hlcr) enterococcus faecalis and enterococcus faecium isolated from fresh produce and fecal samples of patients.201627790716
comparative assessment of antimicrobial efficacy of different antibiotic coated gutta-percha cones on enterococcus faecalis an invitro study.the major goal of endodontic treatment is to eliminate bacteria from the root canals and prevent re-infection. enterococcus faecalis (e. faecalis) has been attributed to be the most common organism for the endodontic treatment failures. the choice of endodontic material that have high antimicrobial efficacy can help in decreasing/avoiding growth of micro-organisms and facilitate the success rate of treatment.201627790583
screening for intestinal colonization with vancomycin resistant enterococci and associated risk factors among patients admitted to an adult intensive care unit of a large teaching hospital.gut colonization with vancomycin resistant enterococci (vre) increases the risk of acquiring infection during hospital stay. patients admitted in the icu's are the major reservoirs for vre colonization due to higher antibiotic pressure.201627790430
apical sealing ability of mineral trioxide aggregate, intermediate restorative material and calcium enriched mixture cement: a bacterial leakage study.this in vitro study compared the apical sealing ability of three common root end filling materials namely mineral trioxide aggregate (mta), intermediate restorative material (irm) and calcium-enriched mixture (cem) cement using a bacterial leakage model.201627790267
antibacterial efficacy of different concentrations of sodium hypochlorite gel and solution on enterococcus faecalis biofilm.this in vitro study compared the antibacterial efficacy of 2.5% sodium hypochlorite gel and 2.5% and 5.25% sodium hypochlorite solutions on enterococcus faecalis (e. faecalis) biofilm.201627790262
the in vitro antibacterial efficacy of persian green tea extract as an intracanal irrigant on enterococcus faecalis biofilm.the aim of this study was to compare the antibacterial effect of persian green tea extract (gte) and 2.5% sodium hypochlorite (naocl) against enterococcus faecalis (e. faecalis) as an intracanal irrigant.201627790260
antibacterial efficacy of calcium hydroxide and chlorhexidine mixture for treatment of teeth with primary endodontic lesions: a randomized clinical trial.this study compared the root canal microbial count of necrotic teeth after irrigation with 6% sodium hypochlorite (naocl) (single session treatment) and two-session root canal treatment with two-week application of calcium hydroxide (ch) mixed with 0.2% chlorhexidine (chx) as intracanal medicament.201627790252
nutrient-induced antibiotic resistance in enterococcus faecalis in the eutrophic environment.nutrient deposition and extensive use of antibiotics are increasing worldwide, especially in freshwater ecosystems. bacteria display resistance to certain antibiotics and thus survive for extended periods in eutrophic environments. in this study, model ecosystems were established to investigate the effect of nitrate and phosphate nutrient salts on antibiotic resistance in strains of enterococcus faecalis. mesocosms were replicated to evaluate the ecological effects of nutrient influx. the mesoco ...201627685672
adjunctive steps for disinfection of the mandibular molar root canal system: a correlative bacteriologic, micro-computed tomography, and cryopulverization approach.this study evaluated the disinfecting ability of chemomechanical preparation with rotary nickel-titanium instruments, followed by 2 distinct adjunctive procedures in the root canals of extracted mandibular molars by means of a correlative analytical approach.201627641947
in vitro antimicrobial effectiveness of root canal sealers against enterococcus faecalis: a systematic review.the purpose of this systematic review was to summarize the outcomes of in vitro studies of root canal sealers for their antimicrobial effectiveness against enterococcus faecalis on the basis of direct contact test.201627623499
influence of smear layer on the antimicrobial activity of a sodium hypochlorite/etidronic acid irrigating solution in infected dentin.the aim of this study was to evaluate the influence of the smear layer on the antimicrobial activity of a 2.5% sodium hypochlorite (naocl)/9% etidronic acid (hebp) irrigating solution against bacteria growing inside dentin tubules.201627616541
comparison of antibacterial effects of 810 and 980- nanometer diode lasers on enterococcus faecalis in the root canal system -an in vitro study.background and aim: use of laser technology in endodontics has greatly increased in the recent years due to the introduction of new wavelengths and methods and optimal antimicrobial and smear layer removal properties of lasers. this in vitro study aimed to compare the antibacterial effects of diode lasers of 810 nm and 980 nm wavelength on enterococcus faecalis (e. faecalis) biofilm in the root canal system. materials and methods: fifty single-canal human anterior teeth were cleaned, shaped, ste ...201627853346
furostanol saponins from the bulbs of welsh onion, allium fistulosum l.an extensive phytochemical analysis of the polar extracts from bulbs of welsh onion allium fistulosum l. led to the isolation of nine saponins, four of them, named fistulosaponins g (1a/1b), h (2), i (3a/3b), and j (4), have never been reported previously. fistulosaponins g and i were isolated as a couple of isomers in equilibrium. on the basis of 2d nmr and mass spectrometry data, the structure of the novel compounds were elucidated as (25r)-26-[(β-d-glucopyranosyl)oxy]-3β,22β-dihydroxyfurost-5 ...201627852096
microbe-mediated host defence drives the evolution of reduced pathogen virulence.microbes that protect their hosts from pathogens are widespread in nature and are attractive disease control agents. given that pathogen adaptation to barriers against infection can drive changes in pathogen virulence, 'defensive microbes' may shape disease severity. here we show that co-evolving a microbe with host-protective properties (enterococcus faecalis) and a pathogen (staphylococcus aureus) within caenorhabditis elegans hosts drives the evolution of reduced pathogen virulence as a by-pr ...201627845328
effects of inactivated enterococcus faecalis on the proliferation and osteogenic induction of osteoblasts.the present study aimed to evaluate the effects of enterococcus faecalis, inactivated by the common intracanal irrigants sodium hypochlorite (naocl) and chlorhexidine (chx), on osteoblasts. e. faecalis was inactivated with 2% chx or 5.25% naocl. subsequently, the cell counting kit‑8 assay was used to examine the effects of chx‑ and naocl-inactivated e. faecalis on mc3t3‑e1 osteoblast cell proliferation. alizarin red staining was used to determine osteoblast mineralization, and osteogenic inducti ...201627840919
bio-fabricated silver nanoparticles preferentially targets gram positive depending on cell surface charge.recently bio-inspired experimental processes for synthesis of nanoparticles are receiving significant attention in nanobiotechnology. silver nanoparticles (ag nps) have been used very frequently in recent times to the wounds, burns and bacterial infections caused by drug-resistant microorganisms. though, the antibacterial effects of ag nps on some multi drug-resistant bacteria specially against gram positive bacteria has been established, but further investigation is needed to elicit its effecti ...201627449536
calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- enterococcus faecalis and mineralization properties.in infected periapical tissues, enterococcus faecalis is one of the most common dominant bacteria. chlorhexidine has been proved to show strong antibacterial ability against e. faecalis but is ineffective in promoting mineralization for tissues around root apex. mesoporous calcium-silicate nanoparticles are newly synthesized biomaterials with excellent ability to promote mineralization and carry-release bioactive molecules in a controlled manner. in this study, mesoporous calcium-silicate nanopa ...201627769275
experience with rapid microarray-based diagnostic technology and antimicrobial stewardship for patients with gram-positive bacteremia.objective to describe the impact of rapid diagnostic microarray technology and antimicrobial stewardship for patients with gram-positive blood cultures. design retrospective pre-intervention/post-intervention study. setting a 1,200-bed academic medical center. patients inpatients with blood cultures positive for staphylococcus aureus, enterococcus faecalis, e. faecium, streptococcus pneumoniae, s. pyogenes, s. agalactiae, s. anginosus, streptococcus spp., and listeria monocytogenes during the 6 ...201627767002
recent horizontal transfer, functional adaptation and dissemination of a bacterial group ii intron.group ii introns are catalytically active rna and mobile retroelements present in certain eukaryotic organelles, bacteria and archaea. these ribozymes self-splice from the pre-mrna of interrupted genes and reinsert within target dna sequences by retrohoming and retrotransposition. evolutionary hypotheses place these retromobile elements at the origin of over half the human genome. nevertheless, the evolution and dissemination of group ii introns was found to be quite difficult to infer.201627765015
poultry blood from slaughterhouses: development of a biopreservation system to improve microbiological quality prior to transforming blood into by-products.the aim of this study was to investigate the use of indigenous lactic acid bacteria (lab) with specific additives as a biopreservation system (bs) for poultry blood during its storage in slaughterhouses. the bs consisted of two lab (enterococcus faecalis dspv 008sa or lactobacillus salivarius dspv 032sa) with 4 additives (lactose 2 g/l, yeast extract 0.4 g/l, ammonium citrate 0.4 g/l and nacl 1 g/l). after 24 h storage at 30ºc, lower counts of enterobacteria, coliforms, pseudomonas spp. and stap ...201627352105
population density modulates drug inhibition and gives rise to potential bistability of treatment outcomes for bacterial infections.the inoculum effect (ie) is an increase in the minimum inhibitory concentration (mic) of an antibiotic as a function of the initial size of a microbial population. the ie has been observed in a wide range of bacteria, implying that antibiotic efficacy may depend on population density. such density dependence could have dramatic effects on bacterial population dynamics and potential treatment strategies, but explicit measures of per capita growth as a function of density are generally not availab ...201627764095
assessment of in vitro antibacterial activity and cytotoxicity effect of nigella sativa oil.methicillin resistance is a serious health concern since it has spread among staphylococcus aureus and coagulase-negative staphylococci (cons) that are frequent community and nosocomial pathogens worldwide. methicillin-resistant strains are often resistant to other classes of antibiotics, making their treatment difficult. nigella sativa oil is known to be active against gram-positive cocci, yet its in vitro cytotoxicity is rarely investigated, is a proper and powerful candidate for treatment of ...201627761077
photodynamic uva-riboflavin bacterial elimination in antibiotic-resistant bacteria.to evaluate the bactericidal effect of clinical ultraviolet a (uva) settings used in photoactivated chromophore for infectious keratitis (pack)-collagen cross-linking (cxl) in antibiotic-resistant and non-resistant bacterial strains.201626867998
evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers.this study evaluated selected properties of a prototype root repair cement containing surface pre-reacted glass ionomer fillers (s-prg) in comparison to mineral trioxide aggregate (mta) and intermediate restorative material (irm).201626787616
high detection rate of the oxazolidinone resistance gene optra in enterococcus faecalis isolated from a chinese anorectal surgery ward. 201627751624
growth behavior of e. coli, enterococcus and staphylococcus species in the presence and absence of sub-inhibitory antibiotic concentrations: consequences for interpretation of culture-based data.culture-based approaches are used to monitor, e.g., drinking water or bathing water quality and to investigate species diversity and antibiotic resistance levels in environmental samples. for health risk assessment, it is important to know whether the growing cultures display the actual abundance of, e.g., clinically relevant antibiotic resistance phenotypes such as vancomycin-resistant enterococcus faecium/enterococcus faecalis (vre) or methicillin-resistant staphylococcus aureus. in addition, ...201627220972
a case of infective endocarditis and pyogenic spondylitis after transrectal ultrasound guided prostate biopsy.we report a patient with infective endocarditis and pyrogenic spondylitis occurring simultaneously. the patient was a 59-year-old man. he was suspected of having prostate cancer due to a high prostate-specific antigen concentration noted in a checkup. he then underwent a transrectal ultrasound guided prostate biopsy with cefotiam as antimicrobial prophylaxis. he had a fever higher than 38 °c and lumbar pain for a few days after the biopsy. enterococcus faecalis was isolated from 2 sets of blood ...201627374863
effects of photodynamic therapy, 2 % chlorhexidine, triantibiotic mixture, propolis and ozone on root canals experimentally infected with enterococcus faecalis: an in vitro study.the aim of this study was to evaluate the antibacterial effect of photodynamic therapy (pdt), 2 % chlorhexidine (chx). the teeth were contaminated with 0.1 ml enterococcus faecalis (3 × 10(8) cell/ml) and randomized into eight treatment groups: group 1 (2.5 % naocl); group 2 (pdt); group 3 (2 % chx); group 4 (tam); group 5 (propolis), group 6 (ozone), group 7 (positive control) and group 8 (negative control). following treatment, the canal content was sampled. the samples were cultured in tripli ...201627771807
enterococcal metabolite cues facilitate interspecies niche modulation and polymicrobial infection.enterococcus faecalis is frequently associated with polymicrobial infections of the urinary tract, indwelling catheters, and surgical wound sites. e. faecalis co-exists with escherichia coli and other pathogens in wound infections, but mechanisms that govern polymicrobial colonization and pathogenesis are poorly defined. during infection, bacteria must overcome multiple host defenses, including nutrient iron limitation, to persist and cause disease. in this study, we investigated the contributio ...201627736645
enterococcus faecalis: e. coli's siderophore-inducing sidekick.many infectious diseases involve polymicrobial infections, which are characterized by synergistic interactions between different microorganisms colonizing a host. in this issue of cell host & microbe, keogh et al. (2016) show that enterococcus faecalis promotes escherichia coli biofilm formation in low-iron conditions, thus facilitating polymicrobial growth.201627736638
multilevel selection of bcrabdr-mediated bacitracin resistance in enterococcus faecalis from chicken farms.in this study we isolated 109 enterococcus faecalis from chicken faecal samples in 6 provinces of china to investigate the prevalence and transmission mechanism of the bacitracin resistance locus bcrabdr in e. faecalis. thirty-seven bcrabdr-positive e. faecalis were detected with 26 different pfge clusters. the mlst of 14 positive strains belonged to st16 and we also detected three new sequence types. s1-pfge analysis indicated that the locus was located on plasmids presenting different sizes, w ...201627731342
antibacterial effect of chlorhexidine-cetrimide combination, salvia officinalis plant extract and octenidine in comparison with conventional endodontic irrigants.the aim of the present study was to compare the antimicrobial effect of sodium hypochlorite (naocl), 2% chlorhexidine (chx), a chx/cetrimide solution (chx+ctr), octenidine hydrochloride (oct) and salvia officinalis plant extract against enterococcus faecalis. seventy decoronated single-rooted human teeth were infected and divided into 6 test (n=10) and 2 control groups (n=5) (negative, sterile samples and positive, infected samples). following irrigants were then applied to test groups: 2.5% nao ...201627725511
bladder catheterization increases susceptibility to infection that can be prevented by prophylactic antibiotic treatment.catheter-associated urinary tract infections (cauti) are the most common hospital-associated infections. here, we report that bladder catheterization initiated a persistent sterile inflammatory reaction within minutes of catheter implantation. catheterization resulted in increased expression of genes associated with defense responses and cellular migration, with ensuing rapid and sustained innate immune cell infiltration into the bladder. catheterization also resulted in hypersensitivity to ente ...201627699248
biodiversity of bacteriophages: morphological and biological properties of a large group of phages isolated from urban sewage.a large scale analysis presented in this article focuses on biological and physiological variety of bacteriophages. a collection of 83 bacteriophages, isolated from urban sewage and able to propagate in cells of different bacterial hosts, has been obtained (60 infecting escherichia coli, 10 infecting pseudomonas aeruginosa, 4 infecting salmonella enterica, 3 infecting staphylococcus sciuri, and 6 infecting enterococcus faecalis). high biological diversity of the collection is indicated by its ch ...201627698408
[effect of lipophosphonoxins on inhibition of bacterial colonization of bone cements].the study aimed at determining the ability of lipophosphonoxin dr5026 to inhibit the formation of bacterial biofilm on the bone cement surface and assessing potential development of bacterial resistance.201627690474
eradication of intracanal enterococcus faecalis biofilm by passive ultrasonic irrigation and rinsendo system.the aim of the study was to compare the antimicrobial efficacy of three irrigation techniques after the use of standardized volume of naocl and with standardized time and irrigation.201627688422
complete genome sequence of enterococcus faecalis strain w11 isolated from an algal food product.here, we report the complete genome sequence of enterococcus faecalis strain w11 isolated from an algal food product in japan. this study should facilitate the identification of a novel mechanism of glycerol metabolic control in lactic acid bacteria.201627688337
draft genome sequences of the probiotic enterococcus faecalis symbioflor 1 clones dsm16430 and dsm16434.the probiotic symbioflor 1 is a historical concoction of 10 isolates of enterococcus faecalis pulsed-field gel electrophoresis revealed two groups: one comprising eight identical clones (dsm16430, dsm16432, dsm16433, dsm16435 to dsm16439) and a further two isolates (dsm16431, dsm16434) with marginally different profiles. here, we report a comparative analysis of the draft genome sequences of representative isolates.201627688319
a rare case of enterococcus faecalis-induced orbital cellulitis and myositis.orbital cellulitis is an infection of soft tissue behind the orbital septum. common pathogens isolated include staphylococcus aureus, staphylococcus epidermidis, and streptococcus pneumoniae. it is a straightforward diagnosis and usually responds to empirical treatment without any sequela. we report a case of orbital cellulitis caused by enterococcus faecalis, which was complicated by myositis of levator palpebrae superioris. to the best of our knowledge, only one case report exists dating way b ...201627688288
prevalence of enterococcus faecalis mediated uti and its current antimicrobial susceptibility pattern in lahore, pakistan.to determine the prevalence of enterococcus faecalis and recent trends in antimicrobial sensitivity profiling.201627686295
new fluoride mi varnish as root canal sealer: an in vitro analysis of bacterial leakage.the aim of the present study was to evaluate and compare the sealing ability of root canal obturation after the application of fluoride varnish (mi varnish) containing 5% sodium fluoride and casein phosphopeptide-amorphous calcium phosphate, fluoride varnish, and ah plus and ah-plus as root canal sealer by bacterial penetration test.201627681400
evaluation of the amoxicillin concentrations in amniotic fluid, placenta, umbilical cord blood and maternal serum two hours after intravenous administration.the aims of this study were to evaluate amoxicillin concentrations in amniotic fluid, placenta, umbilical cord blood and maternal blood two hours after intravenous administration to assess obstetric and non-obstetric factors that could have influences on the penetration of the antibiotic into the examined tissues and to analyze the sensitivity to amoxicillin of the most common pathogens isolated from the genital tract.201628231686
root canal irrigation: chemical agents and plant extracts against enterococcus faecalis.there are various microorganisms related to intra and extra-radicular infections and many of these are involved in persistent infections. bacterial elimination from the root canal is achieved by means of the mechanical action of instruments and irrigation as well as the antibacterial effects of the irrigating solutions. enterococcus faecalis can frequently be isolated from root canals in cases of failed root canal treatments. antimicrobial agents have often been developed and optimized for their ...201628217184
antimicrobial susceptibility patterns: a three-year surveillance study in a rehabilitation setting.to analyze the susceptibility patterns in a rehabilitation center.201628210371
[histological evaluations on periapical tissues after irradiation by erbium-doped yttrium aluminum garnet laser in labradors dogs].to investigate the thermal effects on periapical tissues of labrador dogs after intra-canal irradiation by erbium-doped yttrium aluminum garnet (er:yag) laser at different powers based on the antibacterial experiment of enterococcus faecalis and escherichia coli in root canals with an isthmus, to assess the histological changes, and to prove the safety for clinical applications.201628275785
chemical content, antibacterial and antioxidant properties of essential oil extract from tunisian origanum majorana l. cultivated under saline condition.essential oils of marjoram were extracted from plants, growing under non-saline and saline condition (75mm nacl). their antioxidant and antibaterial activity against six bacteria (enterococcus faecalis, escherichia coli, salmonella enteritidis, listeria ivanovii, listeria inocula, and listeria monocytogenes) were assessed. result showed that, (i) independently of salt treatment, marjoram essential oils inhibited the growth of most of the bacteria but in degrees. the least susceptible one was ent ...201628375110
high frequency of vancomycin resistant enterococcus faecalis in children: an alarming concern.enterococcus spp. is considered as important etiological agents of nosocomial infections. however, a little is known about the epidemiology of vancomycin resistant enterococcus faecalis (vref). the aim of this study was to investigate the frequency of vref and detecting of two prevalent resistance genes (vana, vanb) at children medical center hospital, an iranian referral pediatric hospital.201628167857
prevalence of high-level gentamicin-resistant enterococcus faecalis and enterococcus faecium in an iranian hospital.this study was designed to determine the molecular characteristics and antimicrobial resistance of enterococcal strains isolated from patients admitted to an iranian hospital. enterococcal strains were isolated from the burn patients. all strains were screened for genes encoding resistance to aminoglycoside [aac(6')-ie-aph(2'')-ia, aph (3'), ant (4')], resistance to vancomycin (vana, vanb), resistance to tetracycline (tetk, tetl, tetm, teto), and resistance to erythromycin (erma, ermb, ermc) by ...201628167856
structural proteins of enterococcus faecalis bacteriophage ϕef11.ϕef11, a temperate siphoviridae bacteriophage, was isolated by induction from a root canal isolate of enterococcus faecalis. sequence analysis suggested that the ϕef11 genome included a contiguous 8 gene module whose function was related to head structure assembly and another module of 10 contiguous genes whose products were responsible for tail structure assembly. sds-page analysis of virions of a ϕef11 derivative revealed 11 well-resolved protein bands. to unify the deduced functional gene ass ...201628090386
eradication of enterococcus faecalis biofilms on human dentin.objectives: this work assesses different methods to interfere with enterococcus faecalis biofilms formed on human dentin slabs. methods: first, methods are presented that select for small molecule inhibitors of biofilm targets using multi-well polystyrene biofilm plates. next, we establish methodologies to study and interfere with biofilm formation on a medically relevant model, whereby biofilms are grown on human root dentin slabs. results: non-conventional d-amino acid (d-leucine) can efficien ...201628082955
cinnamaldehyde inhibits staphylococcus aureus virulence factors and protects against infection in a galleria mellonella model.bacterial resistance to the available marketed drugs has prompted the search of novel therapies; especially in regards of anti-virulence strategies that aim to make bacteria less pathogenic and/or decrease their probability to become resistant to therapy. cinnamaldehyde is widely known for its antibacterial properties through mechanisms that include the interaction of this compound with bacterial cell walls. however, only a handful of studies have addressed its effects on bacterial virulence, es ...201628066373
enterococcus faecalis causing delayed spondylodiscitis in a case with retained intraspinal bullet.delayed presentations have been reported following gunshot wounds (gsw) with retained intraspinal bullets due to migration of projectile or lead intoxication. we report on the rare occurrence of delayed pyogenic spondylodiscitis and neurological dysfunction following injury from low velocity gsw to the spine with a retained projectile. a 55-year-old male presented 4 months following gsw to the abdomen which resulted in colonic injury and l5 fracture. the patient was treated initially with ileo-t ...201628097252
antibacterial efficacy of ah plus and ah26 sealers mixed with amoxicillin, triple antibiotic paste and nanosilver.background. elimination of bacteria from the root canal system is one of the aims of endodontic treatment; hence the incorporation of antibiotics into sealers can increase their antimicrobial efficacy. the aim of the present study was to determine the in vitro antimicrobial effects of ah26 and ah plus sealers mixed with amoxicillin, triple antibiotic paste and nanosilver on enterococcus faecalis. methods. in this experiment, amoxicillin, triple antibiotic paste and nanosilver powder were added a ...201628096947
antimicrobial resistance of selected invasive bacteria in a tertiary care center: results of a prospective surveillance study.we aimed to report the distribution and resistance patterns of eight invasive clinically relevant bacteria surveyed in the clinical center of serbia (ccs) in belgrade.201628036312
direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system.we investigated the direct and indirect (residual) antibacterial effects of various concentrations of triple antibiotic paste (tap) loaded into a methylcellulose system. enterococcus faecalis (e. faecalis) was grown on sterilized dentin blocks (n = 60) and treated with clinically used tap (1,000 mg/ml), low concentrations of methylcellulose-based tap (100, 10, and 1 mg/ml), placebo paste, or 1.5% naocl (n = 10). the pastes were then removed, and biofilm disruption assays were performed. addition ...201628025443
rituximab, etoposide, methylprednisolone, high-dose cytarabine, and cisplatin in the treatment of secondary hemophagocytic lymphohistiocytosis with classical hodgkin lymphoma: a case report and review of the literature.hemophagocytic lymphohistiocytosis is becoming an increasingly recognized disorder in adults. classical hodgkin lymphoma is a relatively uncommon etiology of hemophagocytic lymphohistiocytosis and may complicate treatment options. rituximab, etoposide, methylprednisolone, high-dose cytarabine, and cisplatin are discussed here as a treatment regimen.201627998299
bromomethylthioindole inspired carbazole hybrids as promising class of anti-mrsa agents.series of n-substituted carbazole analogues bearing an indole ring were synthesized as anti-methicillin-resistant staphylococcus aureus (mrsa) agents from a molecular hybridization approach. the representative compound 19 showed an mic = 1 μg/ml against a panel of mrsa clinical isolates as it possessed comparable in vitro activities to that of vancomycin. moreover, compound 19 also exhibited mic = 1 μg/ml activities against a recent identified z172 mrsa strain (vancomycin-intermediate and daptom ...201627994762
a novel combination approach of human polyclonal ivig and antibiotics against multidrug-resistant gram-positive bacteria.gram-positive bacteria, especially methicillin-resistant staphylococcus aureus (mrsa) and enterococci, have shown a remarkable ability to develop resistance to antimicrobial agents.201627994476
effect of liquid crystalline systems containing antimicrobial compounds on infectious skin bacteria.this study aimed (i) to prepare liquid crystalline systems (lcs) of glyceryl monooleate (gmo) and water containing antibacterial compounds and (ii) to evaluate their potential as drug delivery systems for topical treatment of bacterial infections. therefore, lcs containing cpc (cetylpyridinium chloride) (lcs/cpc) and phmb (poly(hexamethylene biguanide) hydrochloride) (lcs/phmb) were prepared and the liquid crystalline phases were identified by polarizing light microscopy 24 h and 7 days after pr ...201628028794
the use of different irrigation techniques to decrease bacterial loads in healthy and diabetic patients with asymptomatic apical periodontitis.diabetes mellitus is a multisystem disease which weakens the human's immunity. subsequently, it worsens the sequelae of apical periodontitis by raising a fierce bacterial trait due to the impaired host response.201628028421
pptab exports rgg quorum-sensing peptides in streptococcus.a transposon mutagenesis screen designed to identify mutants that were defective in peptide-pheromone signaling of the rgg2/rgg3 pathway in streptococcus pyogenes generated insertions in sixteen loci displaying diminished reporter activity. fourteen unique transposon insertions were mapped to pptab, an abc-type transporter recently described to export sex pheromones of enterococcus faecalis. consistent with an idea that pptab exports signaling peptides, the pheromones known as shps (short hydrop ...201627992504
broad-range 16s rdna pcr on heart valves in infective endocarditis.infective endocarditis (ie) is diagnosed by blood and/or resected valve cultivation and echocardiographic findings, as defined by the duke criteria. unfortunately, cultures may be negative due to prior antibiotic therapy or fastidious or slow-growing microorganisms. the study aim was to investigate the value of the broad-range polymerase chain reaction (pcr) in addition to blood and valve culture for the detection of causative microorganisms.201627989071
murine experimental root canal infection: cytokine expression in response to f. nucleatum and e. faecalis.the aim of this study was to evaluate the gene expression of proinflammatory (rankl, tnf-a and ifn-g) and regulatory (tgf-b and il-10) cytokines as reaction to experimental infection by mono or bi-association of fusobacterium nucleatum (atcc 10953) and enterococcus faecalis (atcc 19433). f. nucleatum and e. faecalis, either in mono- or bi-association were inoculated into the root canal system (rcs) of balb/c mice. animals were sacrificed at 10 and 20 days after infection and periapical tissues s ...201627982237
effect of silver nanoparticles on physicochemical and antibacterial properties of calcium silicate cements.mineral trioxide aggregate (mta) and portland cement (pc) are calcium silicate cements. they have similar physicochemical, mechanical and biological properties. the addition of zirconium oxide (zro2) to pc provides radiopacity. silver nanoparticles (agnps) may improve some properties of cements. the aim of this study was to evaluate the effect of agnps on physicochemical/mechanical properties and antibacterial activity of white mta (wmta) and pc associated with zro2. the following materials were ...201627982226
draft genome sequence of enterococcus faecalis strain ucd-pd3.here, we present the draft genome sequence of enterococcus faecalis strain ucd-pd3. the assembly contains 2,861,314 bp in 73 contigs. this strain was isolated from a feral domestic cat (felis catus) anal sac secretion sample, as part of a project on isolating and characterizing the microbes present in feline anal sacs.201627979940
antimicrobial resistance in enterococcus strains isolated from healthy domestic dogs.enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. vancomycin and ampicillin minimum inhibitory concentrations (mics) and high-level aminoglycoside resistance (hlar) tests were also performed. isolates showed resist ...201627976593
apical extrusion of intracanal bacteria following use of two engine-driven instrumentation techniques: an in vitro study.the aim of the present study was to compare in vitro the amount of debris extruded apically from extracted teeth, using twisted files and protaper rotary during two different instrumentation systems.201627965505
in vitro evaluation of the sealing ability of three newly developed root canal sealers: a bacterial microleakage study.the purpose of this study was to compare the sealing ability of mta fillapex, apatite root canal sealer and ah26 sealers.201627957271
antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of enterococcus faecalis at different stages of development.persistent infection of the root canal due to the presence of resistance bacterial species, such as enterococcus faecalis, has always been one of the most important reasons for endodontic treatment failure. this study investigated the antimicrobial efficacy of 1%, 2.5 % and 5% sodium hypochlorite in eliminating e. faecalis biofilms at different stages of development.201627957257
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