Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| comparative efficacy of commercial mosquito coils against aedes aegypti (diptera: culicidae) in malaysia: a nationwide report. | this study was conducted using the glass chamber method to determine the susceptibility status of the dengue vector, aedes aegypti (l.) from 11 states in malaysia to commercial mosquito coils containing four different active ingredients, namely metofluthrin, d-allethrin, d-trans allethrin, and prallethrin. aedes aegypti exhibited various knockdown rates, ranging from 14.44% to 100.00%, 0.00% to 61.67%, 0.00% to 90.00%, and 0.00% to 13.33% for metofluthrin, d-allethrin, d-trans allethrin, and pra ... | 2017 | 29048583 |
| evaluation of embryotoxic and embryostatic effects of the aqueous extract of rhizophora mangle and tannic acid on eggs and larvae of aedes aegypti. | rhizophora mangle is an abundant plant in mangroves and tannic acid is a polyphenol produced by the secondary metabolism of plants. the aim of the study was to evaluate the embryotoxic and embriostatic effects of the aqueous extract of r. mangle and synthetic tannic acid on eggs and larvae of aedes aegypti. a. aegypti eggs were exposed in duplicate at concentrations of 250, 500, 750 and 1000 µg/ml of extract and tannic acid for a period of 14 days. mineral water was used as a negative control. t ... | 2017 | 29044325 |
| modeling the environmental suitability for aedes (stegomyia) aegypti and aedes (stegomyia) albopictus (diptera: culicidae) in the contiguous united states. | the mosquitoes aedes (stegomyia) aegypti (l.)(diptera:culicidae) and ae. (stegomyia) albopictus (skuse) (diptera:culicidae) transmit dengue, chikungunya, and zika viruses and represent a growing public health threat in parts of the united states where they are established. to complement existing mosquito presence records based on discontinuous, non-systematic surveillance efforts, we developed county-scale environmental suitability maps for both species using maximum entropy modeling to fit clim ... | 2017 | 29029153 |
| combined toxicity of three essential oils against aedes aegypti (diptera: culicidae) larvae. | essential oils are potential alternatives to synthetic insecticides because they have low mammalian toxicity, degrade rapidly in the environment, and possess complex mixtures of bioactive constituents with multi-modal activity against the target insect populations. twenty-one essential oils were initially screened for their toxicity against aedes aegypti (l.) larvae and three out of the seven most toxic essential oils (manuka, oregano, and clove bud essential oils) were examined for their chemic ... | 2017 | 29029151 |
| effect of climatic conditions and water bodies on population dynamics of the dengue vector, aedes aegypti (diptera: culicidae). | the population of mosquitoes is mainly influenced by the biotic and abiotic factors. although aedes aegypti was reported until 1960's in the punjab, pakistan, the population has increased dramatically since 2009 and caused severe epidemics in 2011 due to heavy floods and rains. thus, this study was carried out to know the effect of biotic and abiotic factors on the population of aedes aegypti. | 2017 | 29026852 |
| central nervous system effects of intrauterine zika virus infection: a pictorial review. | relatively few agents have been associated with congenital infections involving the brain. one such agent is the zika virus, which has caused several outbreaks worldwide and has spread in the americas since 2015. the zika virus is an arbovirus transmitted by infected female mosquito vectors, such as the aedes aegypti mosquito. this virus has been commonly associated with congenital infections of the central nervous system and has greatly increased the rates of microcephaly. ultrasonography (us) ... | 2017 | 29019762 |
| a multiplex pcr for detection of knockdown resistance mutations, v1016g and f1534c, in pyrethroid-resistant aedes aegypti. | mutation of the voltage-gated sodium channel (vgsc) gene, or knockdown resistance (kdr) gene, is an important resistance mechanism of the dengue vector aedes aegypti mosquitoes against pyrethroids. in many countries in asia, a valine to glycine substitution (v1016g) and a phenylalanine to cysteine substitution (f1534c) are common in ae. aegypti populations. the g1016 and c1534 allele frequencies have been increasing in recent years, and hence there is a need to have a simple and inexpensive tool ... | 2017 | 29017613 |
| temporal distribution and insecticide resistance profile of two major arbovirus vectors aedes aegypti and aedes albopictus in yaoundé, the capital city of cameroon. | aedes aegypti and ae. albopictus are the major epidemic vectors of several arbovirus diseases such as yellow fever, dengue, zika and chikungunya worldwide. both aedes vectors are presents in cameroon; however, knowledge on the dynamic of the distribution of these species across cities and their resistance profile to insecticide are limited. here, we assessed the current distribution of ae. aegypti and ae. albopictus in yaoundé, the capital city, established the resistance profile to insecticides ... | 2017 | 29017606 |
| hydrogen peroxide resistance in strigomonas culicis: effects on mitochondrial functionality and aedes aegypti interaction. | reactive oxygen species (ros) are toxic molecules involved in several biological processes such as cellular signaling, proliferation, differentiation and cell death. adaptations to oxidative environments are crucial for the success of the colonization of insects by protozoa. strigomonas culicis is a monoxenic trypanosomatid found in the midgut of mosquitoes and presenting a life cycle restricted to the epimastigote form. among s. culicis peculiarities, there is an endosymbiotic bacterium in the ... | 2017 | 28993269 |
| evaluation of gamma radiation-induced dna damage in aedes aegypti using the comet assay. | the study was undertaken to evaluate gamma radiation-induced dna damage in aedes aegypti. the comet assay was employed to demonstrate the extent of dna damage produced in adult male a. aegypti exposed to seven different doses of gamma radiation, ranging from 1 gy to 50 gy. dna damage was measured as the percentage of comet tail dna. a significant linear increase in dna damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received ... | 2017 | 28992792 |
| how does competition among wild type mosquitoes influence the performance of aedes aegypti and dissemination of wolbachia pipientis? | wolbachia has been deployed in several countries to reduce transmission of dengue, zika and chikungunya viruses. during releases, wolbachia-infected females are likely to lay their eggs in local available breeding sites, which might already be colonized by local aedes sp. mosquitoes. therefore, there is an urgent need to estimate the deleterious effects of intra and interspecific larval competition on mosquito life history traits, especially on the duration of larval development time, larval mor ... | 2017 | 28991902 |
| duplex real-time pcr assay distinguishes aedes aegypti from ae. albopictus (diptera: culicidae) using dna from sonicated first-instar larvae. | aedes aegypti (l.) and ae. albopictus (skuse) are important arbovirus vectors in the united states, and the recent emergence of zika virus disease as a public health concern in the americas has reinforced a need for tools to rapidly distinguish between these species in collections made by vector control agencies. we developed a duplex real-time pcr assay that detects both species and does not cross-amplify in any of the other seven aedes species tested. the lower limit of detection for our assay ... | 2017 | 28981691 |
| pyrethroid susceptibility has been maintained in the dengue vector, aedes aegypti (diptera: culicidae), in queensland, australia. | although pesticide resistance is common in insect vectors of human diseases, the evolution of resistance might be delayed if management practices are adopted that limit selection of resistance alleles. outbreaks of dengue fever have occurred in queensland, australia, since the late 1800s, leading to ongoing attempts to control the mosquito vector, aedes aegypti (l.). since the 1990s, pyrethroid insecticides have been used for this purpose, but have been applied in a strategic manner with a varie ... | 2017 | 28981684 |
| resistance status and resistance mechanisms in a strain of aedes aegypti (diptera: culicidae) from puerto rico. | puerto rico (pr) has a long history of vector-borne disease and insecticide-resistant aedes aegypti (l.). defining contributing mechanisms behind phenotypic resistance is critical for effective vector control intervention. however, previous studies from pr have each focused on only one mechanism of pyrethroid resistance. this study examines the contribution of p450-mediated enzymatic detoxification and sodium channel target site changes to the overall resistance phenotype of ae. aegypti collecte ... | 2017 | 28981681 |
| cutaneous manifestations of chikungunya fever: observations from an outbreak at a tertiary care hospital in southeast rajasthan, india. | chikungunya fever is caused by chikungunya virus which is transmitted by the bite of infected aedes aegypti and a. albopictus mosquitoes. | 2017 | 28979866 |
| infection rate of aedes aegypti mosquitoes with dengue virus depends on the interaction between temperature and mosquito genotype. | dengue fever is the most prevalent arthropod-transmitted viral disease worldwide, with endemic transmission restricted to tropical and subtropical regions of different temperature profiles. temperature is epidemiologically relevant because it affects dengue infection rates in aedes aegypti mosquitoes, the major vector of the dengue virus (denv). aedes aegypti populations are also known to vary in competence for different denv genotypes. we assessed the effects of mosquito and virus genotype on d ... | 2017 | 28978730 |
| identification of surveillance and control priority areas for dengue and other arboviruses transmitted by aedes aegypti in natal-rn, brazil: experience report. | to report the vigi@dengue experience, held in the municipality of natal-rn, brazil, from october/2015 to may/2016. | 2017 | 28977187 |
| circulation of denv2 and denv4 in aedes aegypti (diptera: culicidae) mosquitoes from praia, santiago island, cabo verde. | arthropod-borne viruses, such as dengue (denv), chikungunya (chikv), and zika (zikv), pose a challenge to public health, due to their worldwide distribution and large-scale outbreaks. dengue fever is currently one of the most important diseases and it is caused by four serotypes of denv and is mainly transmitted by the mosquito aedes aegypti. it is estimated that 50-100 million cases are reported every year worldwide. more recently, chikv and zikv, which are also transmitted by ae. aegypti, have ... | 2017 | 28973490 |
| chikungunya virus dissemination from the midgut of aedes aegypti is associated with temporal basal lamina degradation during bloodmeal digestion. | in the mosquito, the midgut epithelium is the initial tissue to become infected with an arthropod-borne virus (arbovirus) that has been acquired from a vertebrate host along with a viremic bloodmeal. following its replication in midgut epithelial cells, the virus needs to exit the midgut and infect secondary tissues including the salivary glands before it can be transmitted to another vertebrate host. the viral exit mechanism from the midgut, the midgut escape barrier (meb), is poorly understood ... | 2017 | 28961239 |
| use of rhodamine b to mark the body and seminal fluid of male aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases. | recent interest in male-based sterile insect technique (sit) and incompatible insect technique (iit) to control aedes aegypti and aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. previous reports from other insects indicated rhodamine b, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. here, we ... | 2017 | 28957318 |
| the distribution of aedes aegypti (diptera, culicidae) in eight selected parks of lahore, using oviposition traps during rainy season. | to investigate the seasonal distribution of immature habitat of aedes aegypti mosquito species and its association with environmental and climatic conditions. | 2017 | 28955062 |
| oviposition preferences of dengue vectors; aedes aegypti and aedes albopictus in sri lanka under laboratory settings. | investigations on oviposition behaviour of dengue vectors are critical for effective controlling of vector breeding. therefore, the aim of the present study was to determine the oviposition behaviour of dengue vectors, aedes aegypti and aedes albopictus in sri lanka. batches of 1000 adult mosquitoes (1 : 1, male: female ratio) housed in rearing cages were used for each experimental setup from ae. aegypti and ae. albopictus. oviposition responses with respect to the size of the ovitrap, colours o ... | 2017 | 28950922 |
| extensive genetic differentiation between homomorphic sex chromosomes in the mosquito vector, aedes aegypti. | mechanisms and evolutionary dynamics of sex-determination systems are of particular interest in insect vectors of human pathogens like mosquitoes because novel control strategies aim to convert pathogen-transmitting females into nonbiting males, or rely on accurate sexing for the release of sterile males. in aedes aegypti, the main vector of dengue and zika viruses, sex determination is governed by a dominant male-determining locus, previously thought to reside within a small, nonrecombining, se ... | 2017 | 28945882 |
| growth inhibitory effect of phenolic extracts of ziziphus jujuba mill. in dengue vector aedes aegypti (l) in parent and f1 generation. | to evaluate the lethal and emergence inhibitory effect of alkaloid, phenolic and terpenoid extracts of ziziphus jujuba (rhamnaceae) against aedes aegypti (diptera: culicidae), and to explore the effect of the most effective fraction on developmental and biochemical parameters of the dengue vector. | 2017 | 28942827 |
| chemical composition of buddleja polystachya aerial parts and its bioactivity against aedes aegypti. | a new acylatediridoid glycoside, 6-o-α-l-(2″-acetyl-4″-o-trans-isoferuloyl) rhamnopyranosyl catalpol (9) together with 18 known compounds belonging to the iridoids, flavonoids, triterpene saponin glycosides and phenylethanoids (1-8, 10-18) were isolated from the aerial parts and the flowers of buddleja polystachya. their structures were elucidated on the basis of spectroscopic evidence and comparison with that reported in the literature. promising adulticidal activity was shown for all extracts ... | 2017 | 28942684 |
| insights into the epidemiological link between biting flies and pemphigus foliaceus in southeastern brazil. | black fly and sandfly bites are related to the endemicity of pemphigus foliaceus (pf); however, an immune reaction against the salivary proteins from these flies still requires confirmation in the case of pf patients living in southeastern brazil. | 2017 | 28941730 |
| contribution of lysinibacillus sphaericus hemolysin and chitin-binding protein in entomopathogenic activity against insecticide resistant aedes aegypti. | given its toxicity against culicid larvae, lysinibacillus sphaericus is used for the biological control of mosquitoes such as culex sp. and anopheles sp. the toxicity factors currently reported for l. sphaericus include the binary toxin, mtx toxins, and the s-layer. however, aedes aegypti is refractory to the binary toxin, the most toxic larvicidal protein of l. sphaericus. until now, there are no evidences of the hemolytic and chitinolytic capacity of l. sphaericus. herein, the expression of th ... | 2017 | 28940156 |
| molecular typing and phylogeny of wolbachia: a study from assam, north-eastern part of india. | wolbachia are maternally inherited endosymbiotic alphaproteobacteria, infecting 40-75% of arthropod species. knowledge on distribution of native strains infecting mosquito vectors from endemic regions is essential for successful implementation of vector control interventions utilizing potential strains of wolbachia. study identified various native strains of wolbachia inhabiting different mosquito species from field and colonised conditions of assam. the fly drosophila melanogaster was also incl ... | 2017 | 28939497 |
| the dengue preface to endemic in mainland china: the historical largest outbreak by aedes albopictus in guangzhou, 2014. | dengue was regarded as a mild epidemic in mainland china transmitted by aedes albopictus. however, the 2014 record-breaking outbreak in guangzhou could change the situation. in order to provide an early warning of epidemic trends and provide evidence for prevention and control strategies, we seek to characterize the 2014 outbreak through application of detailed cases and entomological data, as well as phylogenetic analysis of viral envelope (e) gene. | 2017 | 28934991 |
| isolation and identification of xenorhabdus and photorhabdus bacteria associated with entomopathogenic nematodes and their larvicidal activity against aedes aegypti. | aedes aegypti is a potential vector of west nile, japanese encephalitis, chikungunya, dengue and zika viruses. alternative control measurements of the vector are needed to overcome the problems of environmental contamination and chemical resistance. xenorhabdus and photorhabdus are symbionts in the intestine of entomopathogenic nematodes (epns) steinernema spp. and heterorhabditis spp. these bacteria are able to produce a broad range of bioactive compounds including antimicrobial, antiparasitic, ... | 2017 | 28934970 |
| proteomics technique opens new frontiers in mobilome research. | a large proportion of the genome of most eukaryotic organisms consists of highly repetitive mobile genetic elements. the sum of these elements is called the "mobilome," which in eukaryotes is made up mostly of transposons. transposable elements contribute to disease, evolution, and normal physiology by mediating genetic rearrangement, and through the "domestication" of transposon proteins for cellular functions. although 'omics studies of mobilome genomes and transcriptomes are common, technical ... | 2017 | 28932623 |
| zika virus congenital syndrome: experimental models and clinical aspects. | viral infections have long been the cause of severe diseases to humans, increasing morbidity and mortality rates worldwide, either in rich or poor countries. yellow fever virus, h1n1 virus, hiv, dengue virus, hepatitis b and c are well known threats to human health, being responsible for many million deaths annually, associated to a huge economic and social cost. in this context, a recently introduced flavivirus in south america, called zika virus (zikv), led the who to declare in february 1st 2 ... | 2017 | 28932235 |
| ph control in the midgut of aedesaegypti under different nutritional conditions. | aedes aegypti is one of the most important disease vectors in the world. because their gut is the first site of interaction with pathogens, it is important to understand a. aegypti gut physiology. in this study, we investigated the mechanisms of ph control in the midgut of a. aegypti females under different nutritional conditions. we found that unfed females have an acidic midgut (ph ∼6). the midgut of unfed insects is actively maintained at ph 6 regardless of the ingestion of either alkaline or ... | 2017 | 28931720 |
| microgeographic population structuring of aedes aegypti (diptera: culicidae). | aedes aegypti is one of the species most favored by changes in the environment caused by urbanization. its abundance increases rapidly in the face of such changes, increasing the risk of disease transmission. previous studies have shown that mosquito species that have adapted to anthropogenic environmental changes benefit from urbanization and undergo population expansion. in light of this, we used microsatellite markers to explore how urbanization processes may be modulating ae. aegypti populat ... | 2017 | 28931078 |
| pyrethroid resistance alters the blood-feeding behavior in puerto rican aedes aegypti mosquitoes exposed to treated fabric. | emerging insecticide resistance is a major issue for vector control. it decreases the effectiveness of insecticides, thereby requiring greater quantities for comparable control with a net increase in risk of disease resurgence, product cost, and damage risk to the ecosystem. pyrethroid resistance has been documented in puerto rican populations of aedes aegypti (l.) mosquitoes. in this study, topical toxicity of five insecticides (permethrin, etofenprox, deltamethrin, ddt, transfluthrin) was dete ... | 2017 | 28931018 |
| emerging arboviruses in rio grande do sul, brazil: chikungunya and zika outbreaks, 2014-2016. | the recent emergence of arboviruses such as chikungunya virus (chikv) and zika virus (zikv) in brazil has posed a threat to human health and to the country's economy. outbreaks occur mainly in tropical areas; however, increasing number of cases have been observed in rio grande do sul (rs), the southernmost state; therefore, surveillance of these arboviruses is essential for public health measures. | 2017 | 28929534 |
| sybr green-based one step quantitative real-time polymerase chain reaction assay for the detection of zika virus in field-caught mosquitoes. | the monitoring of vectors is one of the key surveillance measures to assess the risk of arbovirus transmission and the success of control strategies in endemic regions. the recent re-emergence of zika virus (zikv) in the tropics, including singapore, emphasizes the need to develop cost-effective, rapid and accurate assays to monitor the virus spread by mosquitoes. as zikv infections largely remain asymptomatic, early detection of zikv in the field-caught mosquitoes enables timely implementation ... | 2017 | 28927458 |
| insecticide susceptibility of aedes albopictus and ae. aegypti from brazil and the swiss-italian border region. | aedes aegypti and ae. albopictus are two highly invasive mosquito species, both vectors of several viruses, including dengue, chikungunya and zika. while ae. aegypti is the primary vector in the tropics and sub-tropics, ae. albopictus is increasingly under the public health watch as it has been implicated in arbovirus-transmission in more temperate regions, including continental europe. vector control using insecticides is the pillar of most control programmes; hence development of insecticide r ... | 2017 | 28927441 |
| community participation in mosquito breeding site control: an interdisciplinary mixed methods study in curaçao. | as the arboviral diseases dengue, chikungunya and zika emerge in the americas, so does the need for sustainable vector control policies. to successfully achieve mosquito control, joint efforts of both communities and governments are essential. this study investigates this important, but by-and-large neglected topic. | 2017 | 28927437 |
| differential expression of putative sodium-dependent cation-chloride cotransporters in aedes aegypti. | the yellow fever mosquito, aedes aegypti, has three genes that code for proteins with sequence similarity to vertebrate na+-k+-cl- cotransporters (nkccs) of the solute-linked carrier 12 superfamily of cation-chloride cotransporters (cccs). we hypothesized that these mosquito nkcc orthologues have diverged to perform distinct roles in salt secretion and absorption. in phylogenetic analyses, one protein (aenkcc1) groups with a drosophila melanogaster nkcc that mediates salt secretion whereas two o ... | 2017 | 28923771 |
| role of the venus kinase receptor in the female reproductive physiology of the desert locust, schistocerca gregaria. | venus kinase receptors (vkr) are a subfamily of invertebrate receptor tyrosine kinases, which have only recently been discovered. they contain an intracellular tyrosine kinase domain and an extracellular venus flytrap domain. vkrs have been functionally and pharmacologically characterized in only two invertebrate species, namely the human parasite schistosoma mansoni and the mosquito aedes aegypti, where they play a crucial role in oogenesis. here, we report the characterization of a vkr in the ... | 2017 | 28916758 |
| evaluation of ecdysteroid antisera for a competitive enzyme immunoassay and extraction procedures for the measurement of mosquito ecdysteroids. | ecdysteroid hormones regulate several aspects of insect development and reproduction. the predominant ecdysteroids produced by insects including mosquitoes are ecdysone (e) and 20-hydroxyecdysone (20e). the ability to measure e and 20e titers is essential for many studies, but few sensitive, low cost options are currently available for doing so. to address this deficiency, we developed a new enzyme-linked immunoassay (eia). in the first part of the study, we compared the affinity of two new anti ... | 2017 | 28866256 |
| aedes aegypti saliva enhances chikungunya virus replication in human skin fibroblasts via inhibition of the type i interferon signaling pathway. | chikungunya virus (chikv) transmission occurs through the bite of an infected aedes mosquito which injects virus-containing saliva into the skin of the human host during blood feeding. in the present study, we have determined the effect of aedes aegypti saliva on chikv replication in human skin fibroblasts, a major cell type for viral entry, which mimics the events that occur during natural transmission. a significant increase in the expression of viral transcripts and infectious viral particles ... | 2017 | 28866137 |
| presence of a predator image in potential breeding sites and oviposition responses of a dengue vector. | in dengue vector control, attempts to minimize or replace the use of pesticides have mostly involved use of predators, but success has been severely impeded by difficulties associated with financial and environmental costs, predator mass production, and persistence in target habitats. visual deterrents have been used successfully to control animal pests, in some cases in an effort to replace pesticide use. despite evidence that visual signals are crucial in site choice for egg deposition by deng ... | 2017 | 28865898 |
| correction: insecticide resistance to permethrin and malathion and associated mechanisms in aedes aegypti mosquitoes from st. andrew jamaica. | [this corrects the article doi: 10.1371/journal.pone.0179673.]. | 2017 | 28859159 |
| effect of nozzle orientation on dispersion of aqualuer 20-20 sprayed by a truck-mounted ultra-low volume sprayer against caged aedes aegypti. | inconsistencies in efficacy of ultra-low volume (ulv) ground applications in the literature are linked to the lack of adjustments in sprayer parameters. to investigate the effect of nozzle orientation of a truck-mounted ulv sprayer on application efficacy, a study was conducted at st. johns county fairground, elkton, fl, during the summer of 2014. the efficacy was assessed by mortality of caged adult aedes aegypti, spray deposition, and droplet size spectrum up to 122 m from the spray line. aqua ... | 2017 | 28854107 |
| larvicidal and ovicidal activity of terminalia chebula retz. (family: combretaceae) medicinal plant extracts against anopheles stephensi, aedes aegypti and culex quinquefasciatus. | insect-borne diseases remain to this day a major source of illness and death worldwide. the resistance to chemical insecticides among mosquito species has been considered as a setback in vector control. mosquito control programs, botanical origin may have the potential to eliminate eggs and larvae. so, the larvicidal and ovicidal activities of crude benzene, hexane, ethyl acetate, chloroform and methanol extracts of terminalia chebula were assayed for their toxicity against three important vecto ... | 2017 | 28848262 |
| improving the built environment in urban areas to control aedes aegypti-borne diseases. | 2017 | 28804174 | |
| first report of coelomomyces santabrancae sp. nov. (blastocladiomycetes: blastocladiales) infecting mosquito larvae (diptera: culicidae) in central brazil. | a project from 2013 to 2017 sought to discover pathogenic fungi and oomycetes from dipteran species that are vectors of major diseases of humans and animals in central brazil and to begin evaluating the potential of these pathogens as potential biological control agents concentrated on mosquito larvae. some collecting sites proved to be especially productive for pathogens of naturally occurring mosquito species and for placements of healthy sentinel larvae of aedes aegypti in various sorts of co ... | 2017 | 28803981 |
| dinuclear silver(i)-n-heterocyclic carbene complexes: synthesis, characterization and larvicidal activity of bis-imidazolium dinuclear silver(i)-n-heterocyclic carbene complexes. | new synthesized bis-imidazolium salts that are linked by xylyl derivatives moiety, 1-4 was reacted with ag2o to facilitate the formation of dinuclear ag(i)-n-heterocyclic carbene (nhc) complexes, 5-8, respectively. all the synthesized ligand salts and complexes were characterized by 1h and 13c nmr, ftir spectroscopy and elemental analysis. molecular structures of compounds 3, 5, and 7 were elucidated by single crystal x-ray diffraction analyses. larvicidal studies against the aedes aegypti and c ... | 2017 | 28800547 |
| preparation of a nanoemulsion with carapa guianensis aublet (meliaceae) oil by a low-energy/solvent-free method and evaluation of its preliminary residual larvicidal activity. | andiroba (carapa guianensis) seeds are the source of an oil with a wide range of biological activities and ethnopharmacological uses. however, few studies have devoted attention to innovative formulations, including nanoemulsions. the present study aimed to obtain a colloidal system with the andiroba oil using a low-energy and organic-solvent-free method. moreover, the preliminary residual larvicidal activity of the nanoemulsion against aedes aegypti was evaluated. oleic and palmitic acids were ... | 2017 | 28798803 |
| mosquito species occurrence in association with landscape composition in green urban areas. | aedes aegypti prefers densely populated habitats, but has been shown to explore less anthropogenic environments. we investigated composition of the abundance of mosquitoes in forested areas and assessed relationships between species occurrences and different types of land use and land cover at three spatial scales (100m, 500m and 1000m). mosquitoes were collected from october 2012 to march 2013 using oviposition traps. we collected 4,179 mosquitoes in total including at least 10 species. aedes a ... | 2017 | 28793030 |
| insecticide resistance, associated mechanisms and fitness aspects in two brazilian stegomyia aegypti (= aedes aegypti) populations. | in brazil, insecticide resistance in stegomyia aegypti (= aedes aegypti) (diptera: culicidae) populations to pyrethroids and to the organophosphate (op) temephos is disseminated. currently, insect growth regulators (igrs) and the op malathion are employed against larvae and adults, respectively. bioassays with mosquitoes from two northeast municipalities, crato and aracaju, revealed, in both populations, susceptibility to igrs and malathion (rr95 ≤ 2.0), confirming the effectiveness of these co ... | 2017 | 28752548 |
| a mosquito hemolymph odorant-binding protein family member specifically binds juvenile hormone. | juvenile hormone (jh) is a key regulator of insect development and reproduction. in adult mosquitoes, it is essential for maturation of the ovary and normal male reproductive behavior, but how jh distribution and activity is regulated after secretion is unclear. here, we report a new type of specific jh-binding protein, given the name mosquito juvenile hormone-binding protein (mjhbp), which circulates in the hemolymph of pupal and adult aedes aegypti males and females. mjhbp is a member of the o ... | 2017 | 28751377 |
| rhanterium epapposum oliv. essential oil: chemical composition and antimicrobial, insect-repellent and anticholinesterase activities. | the essential oil (eo) of the aerial parts of rhanterium epapposum oliv. (asteraceae), was obtained by hydrodistillation. the oil was subsequently analyzed by both gc-fid and gc-ms, simultaneously. forty-five components representing 99.2% of the oil composition were identified. the most abundant compounds were camphene (38.5%), myrcene (17.5%), limonene (10.1%) and α-pinene (8.7%). referring to the ethnobotanical utilization, an insecticidal assay was performed, where the oil repelled the yellow ... | 2017 | 28725142 |
| effects of rearing salinity on expression and function of ion-motive atpases and ion transport across the gastric caecum of aedes aegypti larvae. | larvae of aedes aegypti, the yellow fever vector, inhabit a variety of aquatic habitats ranging from freshwater to brackish water. this study focuses on the gastric caecum of the larvae, an organ that has not been widely studied. we provide the first measurements of h+, k+ and na+ fluxes at the distal and proximal gastric caecum, and have shown that they differ in the two regions, consistent with previously reported regionalization of ion transporters. moreover, we have shown that the regionaliz ... | 2017 | 28659305 |
| insecticide resistance to permethrin and malathion and associated mechanisms in aedes aegypti mosquitoes from st. andrew jamaica. | the emergence of novel diseases spread by the aedes aegypti mosquito in jamaica and the caribbean, has prompted studies on insecticide resistance towards effective management of the vector. though jamaica has been using the organophosphate insecticide malathion in its vector control program for more than 30 years, resistance to the pesticide has not been tested in over a decade. we analyzed resistance to malathion and the pyrethroid insecticide, permethrin on mosquitoes collected across st. andr ... | 2017 | 28650966 |
| data from salivary gland proteome analysis of female aedes aegypti linn. | salivary gland proteins from female aedes aegypti mosquito were extracted and analyzed on high-resolution mass spectrometry. proteomic data was analysed using two search algorithms sequest and mascot, which results in acquisition of 83,836 spectra which were assigned to 5417 peptides belonging to 1208 proteins.these proteins were then assigned molecular functions and further analysis revealed biological processes they are involved in using gene ontology annotations. several immunity related path ... | 2017 | 28649586 |
| modelling dengue fever risk in the state of yucatan, mexico using regional-scale satellite-derived sea surface temperature. | accurately predicting vector-borne diseases, such as dengue fever, is essential for communities worldwide. changes in environmental parameters such as precipitation, air temperature, and humidity are known to influence dengue fever dynamics. furthermore, previous studies have shown how oceanographic variables, such as el niño southern oscillation (enso)-related sea surface temperature from the pacific ocean, influences dengue fever in the americas. however, literature is lacking on the use of re ... | 2017 | 28450208 |
| detecting the impact of temperature on transmission of zika, dengue, and chikungunya using mechanistic models. | recent epidemics of zika, dengue, and chikungunya have heightened the need to understand the seasonal and geographic range of transmission by aedes aegypti and ae. albopictus mosquitoes. we use mechanistic transmission models to derive predictions for how the probability and magnitude of transmission for zika, chikungunya, and dengue change with mean temperature, and we show that these predictions are well matched by human case data. across all three viruses, models and human case data both show ... | 2017 | 28448507 |
| juvenile hormone and its receptor methoprene-tolerant promote ribosomal biogenesis and vitellogenesis in the aedes aegypti mosquito. | juvenile hormone (jh) controls many biological activities in insects, including development, metamorphosis, and reproduction. in the aedes aegypti mosquito, a vector of dengue, yellow fever, chikungunya, and zika viruses, the metabolic tissue (the fat body, which is an analogue of the vertebrate liver) produces yolk proteins for developing oocytes. jh is important for the fat body to acquire competence for yolk protein production. however, the molecular mechanisms of how jh promotes mosquito rep ... | 2017 | 28446607 |
| species abundance and temporal variation of arbovirus vectors in brownsville, texas. | the recent outbreaks of the dengue fever and west nile viruses and the looming threats of the zika and chikungunya viruses highlight the importance of establishing effective, proactive arboviral surveillance in communities at high risk of transmission, such as those on the texas-mexico border. currently, there are no approved human vaccines available for these mosquito-borne diseases, so entomological control and case management are the only known methods for decreasing disease incidence. the pr ... | 2017 | 28444003 |
| dengue virus replicates and accumulates in aedes aegypti salivary glands. | dengue virus (denv) is an rna virus transmitted among humans by mosquito vectors, mainly aedes aegypti. denv transmission requires viral dissemination from the mosquito midgut to the salivary glands. during this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. despite the implications for virus transmission and evolution, denv replication i ... | 2017 | 28431281 |
| yellow fever from angola and congo: a storm gathers. | in common with zika, chikungunya and dengue, yellow fever (yf) is an arthropod-borne flavivirus. it is transmitted between humans and from monkeys by mosquitoes of the aedes aegypti (its principal vector), haemogogus and albopictus varieties. three cycles of transmission may occur: urban; sylvatic; and intermediate. recently, sub-saharan africa has seen the resurgence of this neglected disease. the current yf outbreak in angola began in december 2015 in the capital luanda and by october 2016 the ... | 2017 | 28424031 |
| detection of a new pyrethroid resistance mutation (v410l) in the sodium channel of aedes aegypti: a potential challenge for mosquito control. | the yellow fever mosquito, aedes aegypti, particularly in neotropical regions, is the principal vector of dengue, yellow fever, zika and chikungunya viruses. pyrethroids remain one of the most used insecticides to control aedes mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. here, we report a brazilian strain of a. aegypti with high levels (approximately 100-60,000 fold) of resistance to both type i and type ii pyrethroids. we detected two mut ... | 2017 | 28422157 |
| deploying dengue-suppressing wolbachia : robust models predict slow but effective spatial spread in aedes aegypti. | a novel strategy for controlling the spread of arboviral diseases such as dengue, zika and chikungunya is to transform mosquito populations with virus-suppressing wolbachia. in general, wolbachia transinfected into mosquitoes induce fitness costs through lower viability or fecundity. these maternally inherited bacteria also produce a frequency-dependent advantage for infected females by inducing cytoplasmic incompatibility (ci), which kills the embryos produced by uninfected females mated to inf ... | 2017 | 28411063 |
| severe dengue due to secondary hemophagocytic lymphohistiocytosis: a case study. | dengue, transmitted by the mosquito aedes aegypti affects millions of people worldwide every year. dengue induced hemophagocytic lymphohistiocytosis (hlh) is a serious condition and may prove fatal if not detected early and treated appropriately. diagnosis of hlh is challenging and usually missed as clinical and laboratory findings are nonspecific. moreover, the pathophysiology of the systemic inflammatory response syndrome and/or sepsis is remarkably similar to hlh. secondary hlh following infe ... | 2017 | 28409119 |
| vector competence and innate immune responses to dengue virus infection in selected laboratory and field-collected stegomyia aegypti (= aedes aegypti). | control of dengue virus (denv) transmission, primarily based on strategies to reduce populations of the principle vector stegomya aegypti (= aedes aegypti) (diptera: culicidae), is difficult to sustain over time. other potential strategies aim to manipulate characteristics such as vector competence (vc), the innate capacity of the vector to transmit the virus. previous studies have identified genetic factors, including differential expression of apoptosis-related genes, associated with the refra ... | 2017 | 28407282 |
| advances in vector control science: rear-and-release strategies show promise… but don't forget the basics. | both chikungunya and zika viruses have recently swept from africa across the pacific to the americas, causing major outbreaks of disease in humans. in the meantime, dengue epidemics continue throughout the tropics. traditional vector control programs based on strategies from 1950s and 1960s have been relatively ineffective in combating recent epidemics. in response, new methods involving the rearing and releasing of large numbers of mosquitoes to eliminate or modify local aedes populations are b ... | 2017 | 28403439 |
| aedes aegypti (diptera: culicidae) abundance model improved with relative humidity and precipitation-driven egg hatching. | we propose an improved aedes aegypti (l.) abundance model that takes into account the effect of relative humidity (rh) on adult survival, as well as rainfall-triggered egg hatching. the model uses temperature-dependent development rates described in the literature as well as documented estimates for mosquito survival in environments with high rh, and for egg desiccation. we show that combining the two additional components leads to better agreement with surveillance trap data and with dengue inc ... | 2017 | 28402546 |
| repellency of 29 synthetic and natural commercial topical insect repellents against aedes aegypti (diptera: culicidae) in central mexico. | in mexico, the use of repellents to prevent insects from landing and biting is a common practice. however, variation in the efficiency of natural and synthetic repellents has been observed. in this study, we evaluated the repellency and protection time of 16 synthetic and 13 natural-based commercial products against aedes aegypti (l.) from an endemic dengue area (jojutla, morelos) in central mexico. the "arm exposure" cage test was used to assess the efficacy of the repellents. tests were conduc ... | 2017 | 28402436 |
| why is aedes aegypti linnaeus so successful as a species? | diseases transmitted by mosquitoes impose enormous burden towards human morbidity and mortality. over the last three decades, brazil has suffered from severe dengue epidemics. in september 2014, this situation is further complicated by the introduction of two other viruses, zika and chikungunya, placing brazil in a triple epidemic. in this article, we discuss the biology of aedes aegypti linnaeus, and the principal initiatives currently used to control mosquito populations and the diseases they ... | 2017 | 28401481 |
| zika virus alters the microrna expression profile and elicits an rnai response in aedes aegypti mosquitoes. | zika virus (zikv), a flavivirus transmitted primarily by aedes aegypti, has recently spread globally in an unprecedented fashion, yet we have a poor understanding of host-microbe interactions in this system. to gain insights into the interplay between zikv and the mosquito, we sequenced the small rna profiles in zikv-infected and non-infected ae. aegypti mosquitoes at 2, 7 and 14 days post-infection. zika induced an rnai response in the mosquito with virus-derived short interfering rnas and piwi ... | 2017 | 28715413 |
| biopolymer zein-coated gold nanoparticles: synthesis, antibacterial potential, toxicity and histopathological effects against the zika virus vector aedes aegypti. | the control of multidrug-resistant bacteria as well as insect pests and vectors is timely and important now a days. the present study was designed to evaluate the in vitro antibacterial, antibiofilm and mosquito larvicidal effects of gold nanoparticles synthesized using the zein biopolymer (ze-aunps) against gram positive (bacillus pumilus and bacillus subtilis), gram negative (shigella sonnei and pseudomonas aeruginosa) bacteria and third instar larvae of the dengue and zika virus vector aedes ... | 2017 | 28654862 |
| cell fusing agent virus and dengue virus mutually interact in aedes aegypti cell lines. | the genus flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne rna viruses. some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (denv), west nile virus, and yellow fever virus. these viruses are transmitted to vertebrates by mosquitoes and other arthropod species. mosquitoes are also infected by insect-specific flaviviruses (isfs) that do not appear to be infective to vertebrates. cell fusing agent virus (cfav) ... | 2017 | 28761113 |
| experimental infection with and maintenance of cell fusing agent virus (flavivirus) in aedes aegypti. | during the past two decades, there has been a dramatic increase in the recognition and characterization of novel insect-specific flaviviruses (isfvs). some of these agents are closely related to important mosquito-borne flavivirus pathogens. results of experimental studies suggest that mosquitoes and mosquito cell cultures infected with some isfvs are refractory to superinfection with related flavivirus pathogens; and it has been proposed that isfvs potentially could be used to alter the vector ... | 2017 | 28719335 |
| activation of aedes aegypti prophenoloxidase-3 and its role in the immune response against entomopathogenic fungi. | serine protease cascade-mediated melanization is an important innate immune response in insects and crustaceans, which involves the proteolytic activation of prophenoloxidase (ppo). in this study, we investigated the role of aedes aegypti ppo3 in antifungal immune defence. we expressed and purified recombinant ppo3 (rppo3) in escherichia coli and demonstrated that rppo3 was activated by ethanol and, to a lesser extent, by cetylpyridinium chloride. in the presence of cu(2+) , rppo3 exhibited enzy ... | 2017 | 28556276 |
| understanding the role of micrornas in the interaction of aedes aegypti mosquitoes with an insect-specific flavivirus. | the flavivirus genus contains some of the most prevalent vector-borne viruses, such as the dengue, zika and yellow fever viruses that cause devastating diseases in humans. however, the insect-specific clade of flaviviruses is restricted to mosquito hosts, albeit they have retained the general features of the genus, such as genome structure and replication. the interactions between insect-specific flaviviruses (isfs) and their mosquito hosts are largely unknown. pathogenic flaviviruses are known ... | 2017 | 28699859 |
| synergistic efficacy of aedes aegypti antimicrobial peptide cecropin a2 and tetracycline against pseudomonas aeruginosa. | the increasing prevalence of antibiotic resistance has created an urgent need for alternative drugs with new mechanisms of action. antimicrobial peptides (amps) are promising candidates that could address the spread of multidrug-resistant bacteria, either alone or in combination with conventional antibiotics. we studied the antimicrobial efficacy and bactericidal mechanism of cecropin a2, a 36-residue α-helical cationic peptide derived from aedes aegypti cecropin a, focusing on the common pathog ... | 2017 | 28483966 |
| aedes aegypti piwi4 is a noncanonical piwi protein involved in antiviral responses. | the small interfering rna (sirna) pathway is a major antiviral response in mosquitoes; however, another rna interference pathway, the piwi-interacting rna (pirna) pathway, has been suggested to be antiviral in mosquitoes. piwi4 has been reported to be a key mediator of this response in mosquitoes, but it is not involved in the production of virus-specific pirnas. here, we show that piwi4 associates with members of the antiviral exogenous sirna pathway (ago2 and dcr2), as well as with proteins of ... | 2017 | 28497119 |
| characterization of tolypocladium cylindrosporum (hypocreales: ophiocordycipitaceae) and its impact against aedes aegypti and aedes albopictus eggs at low temperature. | we examined the growth characteristics of tolypocladium cylindrosporum ibt 41712 and its potential to infect eggs of aedes aegypti and ae. albopictus at a low temperature (15°c). when grown on sabouraud dextrose agar supplemented with yeast extract, the ibt 41712 formed white colonies turning to a slightly darker, off-white color when mature. the mycelia bore swollen conidiophores producing smooth-walled, oblong to cylindrical conidia with varying sizes, ranging from 1.5 to 3.5 μm long. to deter ... | 2017 | 28854112 |
| the role of the environment in the evolution of tolerance and resistance to a pathogen. | defense against parasites can be divided into resistance, which limits parasite burden, and tolerance, which reduces pathogenesis at a given parasite burden. distinguishing between the two and understanding which defense is favored by evolution in different ecological settings are important, as they lead to fundamentally different evolutionary trajectories of host-parasite interactions. we let the mosquito aedes aegypti evolve under different food levels and with either no parasite, a constant p ... | 2017 | 28829641 |
| specificity and putative mode of action of a mosquito larvicidal toxin from the bacterium xenorhabdus innexi. | reduction of mosquito-borne diseases relies, in part, on the use of synthetic pesticides to control pest mosquitoes. this reliance has led to genetic resistance, environmental contamination and the nondiscriminatory elimination of both pest and non-pest species. to expand our options for control, we screened entomopathogenic bacteria for potential larvicidal activity. a lipopeptide from the bacterium, xenorhabdus innexi, was discovered that displayed potent larvicidal activity. the lc50s of the ... | 2017 | 28712711 |
| potential risk of re-emergence of urban transmission of yellow fever virus in brazil facilitated by competent aedes populations. | yellow fever virus (yfv) causing a deadly viral disease is transmitted by the bite of infected mosquitoes. in brazil, yfv is restricted to a forest cycle maintained between non-human primates and forest-canopy mosquitoes, where humans can be tangentially infected. since late 2016, a growing number of human cases have been reported in southeastern brazil at the gates of the most populated areas of south america, the atlantic coast, with rio de janeiro state hosting nearly 16 million people. we sh ... | 2017 | 28687779 |
| taking innovative vector control interventions in urban latin america to scale: lessons learnt from multi-country implementation research. | prior to the current public health emergency following the emergence of chikungunya and zika virus disease in the americas during 2014 and 2015, multi-country research investigated between 2011 and 2013 the efficacy of novel aedes aegypti intervention packages through cluster randomised controlled trials in four latin-american cities: fortaleza (brazil); girardot (colombia), acapulco (mexico) and salto (uruguay). results from the trials led to a scaling up effort of the interventions at city lev ... | 2017 | 28829235 |
| modeling the transmission and control of zika in brazil. | zika virus, a reemerging mosquito-borne flavivirus, started spread across central and southern america and more recently to north america. the most serious impacted country is brazil. based on the transmission mechanism of the virus and assessment of the limited data on the reported suspected cases, we establish a dynamical model which allows us to estimate the basic reproduction number r 0 = 2.5020. the wild spreading of the virus make it a great challenge to public health to control and preven ... | 2017 | 28798323 |
| differential outcomes of zika virus infection in aedes aegypti orally challenged with infectious blood meals and infectious protein meals. | infection of mosquitoes is an essential step for the transmission of mosquito-borne arboviruses in nature. engorgement of infectious blood meals from viremic infected vertebrate hosts allows the entry of viruses and initiates infection of midgut epithelial cells. historically, the infection process of arboviruses in mosquitoes has been studied through the engorgement of mosquitoes from viremic laboratory animals or from artificial feeders containing blood mixed with viruses harvested from cell c ... | 2017 | 28796799 |
| zika virus replication in the mosquito culex quinquefasciatus in brazil. | zika virus (zikv) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. the virus is primarily transmitted by mosquito bite, although other routes of infection have been implicated in some cases. the aedes aegypti mosquito is considered to be the main vector to humans worldwide; however, there is evidence that other mosquito species, including culex quinquefasciatus, transmit the virus. to test the potential of cx ... | 2017 | 28790458 |
| sexual and vertical transmission of zika virus in anti-interferon receptor-treated rag1-deficient mice. | although zika virus (zikv) is primarily transmitted to humans by the aedes aegypti mosquito, human-to-human transmission has also been observed from males-to-females as well as mother-to-offspring. in the current study, we studied both sexual transmission (stx) and vertical transmission (vtx) of zikv using anti-ifnar1-treatment of rag1 (-/-) (air) mice. these mice have suppressed type i ifn responses and lack adaptive immune responses, leading to a prolonged infection prior to clinical disease. ... | 2017 | 28775298 |
| culex quinquefasciatus from areas with the highest incidence of microcephaly associated with zika virus infections in the northeast region of brazil are refractory to the virus. | zika virus (zikv) is widely distributed in brazil and the northeast region (ne) is the most affected zone, showing the highest incidence of microcephaly associated with zikv congenital infections worldwide. we report attempts to infect three populations of culex quinquefasciatus from severely affected sites in the ne and southeast region (se) of brazil with three strains of zikv isolated from these localities. an aedes aegypti population from the se was used as a positive control. all tested cx. ... | 2017 | 28767975 |
| linking only aedes aegypti with zika virus has world-wide public health implications. | 2017 | 28736548 | |
| zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process. | zika disease has transformed into a serious global health problem due to the rapid spread of the arbovirus and alarming severity including congenital complications, microcephaly and guillain-barré syndrome. zika virus (zikv) is primarily transmitted to humans through the bite of an infective mosquito, with aedes aegypti being the main vector. | 2017 | 28728607 |
| asymptomatic transmission and the dynamics of zika infection. | following the 2013-14 outbreak in french polynesia, the zika virus (zikv) epidemic spread widely to many countries where aedes aegypti as the main transmitting vector is endemic. the lack of a second wave of zikv infection in most affected regions may suggest that a sufficiently high level of herd immunity was reached during the first wave. we developed an agent-based transmission model to investigate the role of asymptomatic infection on the likelihood of observing a second wave, while accounti ... | 2017 | 28724972 |
| american aedes vexans mosquitoes are competent vectors of zika virus. | abstractstarting in 2013-2014, the americas have experienced a massive outbreak of zika virus (zikv) which has now reached at least 49 countries. although most cases have occurred in south america and the caribbean, imported and autochthonous cases have occurred in the united states. aedes aegypti and aedes albopictus mosquitoes are known vectors of zikv. little is known about the potential for temperate aedes mosquitoes to transmit zikv. aedes vexans has a worldwide distribution, is highly abun ... | 2017 | 28719283 |
| early evidence for zika virus circulation among aedes aegypti mosquitoes, rio de janeiro, brazil. | during 2014-2016, we conducted mosquito-based zika virus surveillance in rio de janeiro, brazil. results suggest that zika virus was probably introduced into the area during may-november 2013 via multiple in-country sources. furthermore, our results strengthen the hypothesis that zika virus in the americas originated in brazil during october 2012-may 2013. | 2017 | 28628464 |
| a single substitution changes zika virus infectivity in mosquitoes. | zika virus (zikv) has caused outbreaks in the pacific and the americas. the mechanism underlying the recent zikv epidemic remains obscure. a recent study reveals that an amino acid substitution is associated with increased infectivity of zikv in the aedes aegypti mosquito. | 2017 | 28610876 |
| zika vector transmission risk in temperate australia: a vector competence study. | zika virus is an emerging pathogen of global importance. it has been responsible for recent outbreaks in the americas and in the pacific region. this study assessed five different mosquito species from the temperate climatic zone in australia and included aedes albopictus as a potentially invasive species. | 2017 | 28599659 |
| emerging infectious disease agents and blood safety in australia: spotlight on zika virus. | emerging infectious diseases (eids) are infectious diseases whose incidence has increased in humans in the past 20 years or could increase in the near future. eid agents may represent a threat to blood safety if they infect humans, cause a clinically significant illness, include an asymptomatic blood phase in the course of infection, and are transmissible by transfusion. eid agents are typically not well characterised, but there is a consensus that we can expect ongoing outbreaks. strategies to ... | 2017 | 28566073 |
| genomic epidemiology reveals multiple introductions of zika virus into the united states. | zika virus (zikv) is causing an unprecedented epidemic linked to severe congenital abnormalities. in july 2016, mosquito-borne zikv transmission was reported in the continental united states; since then, hundreds of locally acquired infections have been reported in florida. to gain insights into the timing, source, and likely route(s) of zikv introduction, we tracked the virus from its first detection in florida by sequencing zikv genomes from infected patients and aedes aegypti mosquitoes. we s ... | 2017 | 28538723 |
| de novo generation and characterization of new zika virus isolate using sequence data from a microcephaly case. | zika virus (zikv) has recently emerged and is the etiological agent of congenital zika syndrome (czs), a spectrum of congenital abnormalities arising from neural tissue infections in utero. herein, we describe the de novo generation of a new zikv isolate, zikvnatal, using a modified circular polymerase extension reaction protocol and sequence data obtained from a zikv-infected fetus with microcephaly. zikvnatal thus has no laboratory passage history and is unequivocally associated with czs. zikv ... | 2017 | 28529976 |