Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| bacterial aetiology of diarrhoeal diseases and antimicrobial resistance in dhaka, bangladesh, 2005-2008. | summaryinfectious diarrhoea caused by bacterial pathogens contributes to the high level of mortality in developing countries like bangladesh. following standard bacteriological procedures, a total of 14 428 bacterial pathogens were isolated from 56 132 stool samples and rectal swabs collected from diarrhoeal patients between 2005 and 2008. the rate of isolation and antimicrobial susceptibility data were retrospectively analysed for these isolates and among them vibrio spp. (42·9%) were the most ... | 2011 | 22040529 |
| [Antibacterial activity of nanostructured silver gel]. | Evaluation of antimicrobial activity of L-cysteine silver gel against various species of pathogenic and opportunistic microorganisms. | 2011 | 21913399 |
| Control of an outbreak of diarrhoea in a vascular surgery unit caused by a high-level clindamycin-resistant Clostridium difficile PCR ribotype 106. | This report describes an outbreak of Clostridium difficile infection (CDI) in a vascular surgery ward in 2009 caused by a high-level clindamycin-resistant ribotype 106. A case of CDI was defined as a patient with diarrhoea, positive for C. difficile toxin and negative for other enteric pathogens. Cultures were sent to the Scottish Salmonella Shigella and Clostridium difficile Reference Laboratory (SSSCDRL) for PCR ribotyping, antibiotic susceptibility testing and PCR detection of ermB. The mean ... | 2011 | 21864938 |
| phylogenetic characterization of bacteria in the gut of house flies (musca domestica l.). | house flies (musca domestica l.) are cosmopolitan, ubiquitous, synanthropic insects that serve as mechanical or biological vectors for various microorganisms. to fully assess the role of house flies in the epidemiology of human diseases, it is essential to understand the diversity of microbiota harbored by natural fly populations. this study aimed to identify the diversity of house fly gut bacteria by both culture-dependent and culture-independent approaches. a total of 102 bacterial strains wer ... | 2011 | 22092755 |
| dipstick test for rapid diagnosis of shigella dysenteriae 1 in bacterial cultures and its potential use on stool samples. | we describe a test for rapid detection of s. dysenteriae 1 in bacterial cultures and in stools, at the bedside of patients. | 2011 | 21984895 |
| antimicrobial resistance of shigella spp. isolated in the state of pará, brazil. | shigella spp. are gram-negative, nonsporulating, rod-shaped bacteria that belong to the family enterobacteriaceae and are responsible for shigellosis or bacillary dysentery, an important cause of worldwide morbidity and mortality. | 2011 | 21860994 |
| wide dissemination of multidrug-resistant shigella isolates in china. | to evaluate the prevalence of antimicrobial resistance and selected antimicrobial resistance mechanisms in shigella isolates recovered in outpatients from 1997 to 2009 in china. | 2011 | 21859815 |
| Sepsis-related myocardial calcification. | 2011 | 21934087 | |
| recombinant shiga toxin b subunit elicits protection against shiga toxin via mixed th type immune response in mice. | shigella dysenteriae is the causative agent of the third commonest bacterial disease for childhood diarrhoea and responsible for millions of deaths per year. it produces potent toxin termed shiga toxin which is listed in category b biological warfare agent of cdc, usa. earlier we have reported production of recombinant shiga toxin b subunit that produced antibodies which neutralized shiga toxin toxicity in hela cells. in the present study, we have evaluated the immunomodulatory potential of rstx ... | 2011 | 21856355 |
| Acid-bile, antibiotic resistance and inhibitory properties of propionibacteria isolated from Turkish traditional home-made cheeses. | In this study, a total of 29 Propionibacterium spp. were isolated from traditional home-made Turkish cheese samples. As a result of the identification, isolates were identified as Propionibacterium freudenreichii subsp. freudenreichii (15 strains), Propionibacterium jensenii (12), and Propionibacterium thoenii (2). All isolates and 5 reference strains were examined for their abilities to survive at pH 2.0, 3.0, 4.0, 5.0 and in the presence of 0.06, 0.15 and 0.30% bile salts, their influence on t ... | 2011 | 22019987 |
| Prevalence and antimicrobial susceptibility of major foodborne pathogens in imported seafood. | Seafood is a leading commodity implicated in foodborne disease outbreaks in the United States. Seafood importation rose dramatically in the past 3 decades and now contributes to more than 80% of the total U.S. seafood supply. However, limited data are available on the microbiological safety of imported seafood. In this study, we obtained a total of 171 salmon, shrimp, and tilapia samples imported from 12 countries in three retail stores in Baton Rouge, LA. The total microbial population and the ... | 2011 | 21902913 |
| a broadly protective shigella vaccine based on type iii secretion apparatus proteins. | shigella ssp. are food and water-borne pathogens that cause severe diarrheal and dysenteric disease associated with high morbidity and mortality. individuals most often affected are children under 5 years of age in the developing world. the existence of multiple shigella serotypes and the heterogenic distribution of pathogenic strains as well as emerging antibiotic resistance require the development of a broadly protective vaccine. all shigella ssp. utilize a type iii secretion system (ttss) to ... | 2011 | 22202122 |
| up-regulation of muc2 and il-1β expression in human colonic epithelial cells by shigella and its interaction with mucins. | the entire gastrointestinal tract is protected by a mucous layer, which contains complex glycoproteins called mucins. muc2 is one such mucin that protects the colonic mucosa from invading microbes. the initial interaction between microbes and mucins is an important step for microbial pathogenesis. hence, it was of interest to investigate the relationship between host (mucin) and pathogen interaction, including shigella induced expression of muc2 and il-1β during shigellosis. | 2011 | 22073249 |
| Molecular Techniques in Ecohealth Research Toolkit: Facilitating Estimation of Aggregate Gastroenteritis Burden in an Irrigated Periurban Landscape. | Assessment of microbial hazards associated with certain environmental matrices, livelihood strategies, and food handling practices are constrained by time-consuming conventional microbiological techniques that lead to health risk assessments of narrow geographic or time scope, often targeting very few pathogens. Health risk assessment based on one or few indicator organisms underestimates true disease burden due a number of coexisting causative pathogens. Here, we employed molecular techniques i ... | 2011 | 22146856 |
| [Protozoal antigen positivity in diarrheal patients admitted to emergency service: a point prevalence study]. | Intestinal parasites are the important etiological agents of water and food related diarrhea cases which are frequently seen during summer/early autumn seasons in developing countries. This point prevalence study was aimed to determine the protozoal antigen positivity rate in diarrhea cases admitted to the emergency service in one single day. A total of 198 diarrheal patients (90 male, 108 female; age range: 1-82 years, mean age: 29 years) who were admitted to the emergency service of Ankara Tra ... | 2011 | 22090311 |
| interaction of concanavalin a with bacterial lipopolysaccharides in agarose gel. | binding of fluorescein isothiocyanate-labeled concanavalin a to a series of molecular species of lipopolysaccharide (lps), purified from pathogenic bacteria, was studied via agarose gel precipitation experiments and the results were compared with available structural data.the lps species could be divided into cona-reactive and non-reactive ones. reactivity resided in the o-specific chain of lps, and binding to the lipid a or core moieties of lps could not be demonstrated by the present methods. ... | 2011 | 21983321 |
| [Etiological aspects of acute gastroenteritis--a ten-year review (1.01. 2001-31.12.2010)]. | Acute gastroentritis is one of the most common diseases in humans, and continues to be a significant cause of morbidity worldwide. | 2011 | 22046776 |
| comparative genomic analysis of bacteriophage ep23 infecting shigella sonnei and escherichia coli. | bacteriophage ep23 that infects escherichia coli and shigella sonnei was isolated and characterized. the bacteriophage morphology was similar to members of the family siphoviridae. the 44,077 bp genome was fully sequenced using 454 pyrosequencing. comparative genomic and phylogenetic analyses showed that ep23 was most closely related to phage so-1, which infects sodalis glossinidius and phage ssl-2009a, which infects engineered e. coli. genomic comparison indicated that ep23 and so-1 were very s ... | 2011 | 22203555 |
| [Antimicrobial resistance profiles of Shigella spp. isolated from feces samples in Hacettepe University Ihsan Dogramaci Children's Hospital between 1999-2010]. | The symptoms of infections caused by Shigella spp. are diverse and may change from person to person. The choice of antibiotics as well as the prevention of the loss of fluid and electrolytes are important in the clinical recovery. The local resistance rates to antibiotics should be taken into consideration when planning empirical therapy. The aims of this retrospective study were to detect the in vitro antimicrobial susceptibility of 605 Shigella spp. strains isolated from feces samples of child ... | 2011 | 22090291 |
| site-specific incorporation of photo-cross-linker and bioorthogonal amino acids into enteric bacterial pathogens. | enteric bacterial pathogens are known to effectively pass through the extremely acidic mammalian stomachs and cause infections in the small and/or large intestine of human hosts. however, their acid-survival strategy and pathogenesis mechanisms remain elusive, largely due to the lack of tools to directly monitor and manipulate essential components (e.g., defense proteins or invasive toxins) participating in these processes. herein, we have extended the pyrrolysine-based genetic code expansion st ... | 2011 | 22084898 |
| cloning, characterization and evaluation of potent inhibitors of shigella sonnei acetohydroxyacid synthase catalytic subunit. | acetohydroxyacid synthase (ahas) is a thiamin diphosphate (thdp)- and flavin adenine dinucleotide (fad)-dependent plant and microbial enzyme that catalyzes the first common step in the biosynthesis of essential amino acids such as leucine, isoleucine and valine. to identify strong potent inhibitors against shigella sonnei (s. sonnei) ahas, we cloned and characterized the catalytic subunit of s. sonnei ahas and found two potent chemicals (khg20612, khg25240) that inhibit 87-93% s. sonnei ahas act ... | 2011 | 22015678 |
| systematic review: the use of proton pump inhibitors and increased susceptibility to enteric infection. | background: the use of proton pump inhibitors (ppis) is increasing worldwide. suppression of gastric acid alters the susceptibility to enteric bacterial pathogens. aim this systematic review was undertaken to examine the relationship between ppi use and susceptibility to enteric infections by a specific pathogen based on published literature and to discuss the potential mechanisms of ppi enhanced pathogenesis of enteric infections. methods pubmed, ovid medline databases were searched. search t ... | 2011 | 21999643 |
| liver mass in a young adult. | 2011 | 21943702 | |
| shigella flexneri infection generates the lipid pi5p to alter endocytosis and prevent termination of egfr signaling. | the phosphoinositide metabolic pathway, which regulates cellular processes implicated in survival, motility, and trafficking, is often subverted by bacterial pathogens. shigella flexneri, a bacterium that causes dysentery, injects ipgd, a phosphoinositide phosphatase that generates the lipid phosphatidylinositol 5-phosphate (pi5p), into host cells, thereby activating the phosphoinositide 3-kinase-akt survival pathway. we show that epidermal growth factor receptor (egfr) is required for pi5p-depe ... | 2011 | 21934107 |
| genomic characterization of enteroaggregative escherichia coli from children in mali. | background. enteroaggregative escherichia coli (eaec) is a cause of epidemic and sporadic diarrhea, yet its role as an enteric pathogen is not fully understood. methods. we characterized 121 eaec strains isolated in 2008 as part of a case-control study of moderate to severe acute diarrhea among children 0-59 months of age in bamako, mali. we applied multiplex polymerase chain reaction and comparative genome hybridization to identify potential virulence factors among the eaec strains, coupled wit ... | 2011 | 22184729 |
| Nicotinamide dependence of uropathogenic Escherichia coli UTI89 and application of nadB as a neutral insertion site. | NAD and NADP function ubiquitously in the metabolism of Escherichia coli K-12. NAD auxotrophy can be rendered by mutation in any of the three genes, nadB, nadA, and nadC. The nadB and nadA genes were defined as anti-virulence loci in Shigella spp., as a mutation (mainly in nadB) disrupting the synthesis of quinolinate is required for virulence. Uropathogenic E. coli (UPEC) isolates, from acute cystitis patients, exhibiting nicotinamide auxotrophy were of serotype O18:K1:H7. E. coli UTI89, the mo ... | 2011 | 22174382 |
| antibacterial effect and cytotoxicity of beta-1,3-1, 4-glucanase from endophytic bacillus subtilis swb8. | we studied the antibiotic activity and selective cytotoxicity of beta-1,3-1,4-glucanase from endophytic bacillus subtilis swb8. | 2011 | 22260051 |
| Molecular mechanism of multi-drug resistance in Shigella isolates from rural China. | Diarrhoeal diseases and enteric infections are major causes of morbidity and mortality in the developing countries. Shigellosis was sixth in the rank of death caused by infectious disease in China. Present study assess the patterns of antimicrobial susceptibility and mutations in marA genes of Shigella isolates and its association. One hundred isolates of Shigella spp were tested to evaluate the antimicrobial susceptibility and mutation of marA through PCR-SSCP. The antimicrobial resistance prof ... | 2011 | 21991697 |
| culture-independent real-time pcr reveals extensive polymicrobial infections in hospitalized diarrhoea cases in kolkata, india. | clin microbiol infect abstract: culture-independent identification of diarrhoeal aetiological agents was performed using dna harvested from diarrhoeal stool specimens with sybr-green-based real-time pcr targeting vibrio cholerae, vibrio parahaemolyticus, campylobacter spp., shigella spp. and three different pathotypes of diarrhoeagenic escherichia coli. conventional culture-dependent methods detected bacterial enteropathogens in 68 of 122 diarrhoeal stool specimens. of 68 specimens, 59 (86.8%) h ... | 2011 | 22268636 |
| synthesis and biological evaluation of some thiazolidinones as antimicrobial agents. | a novel series of thiazolidinone derivatives namely 4-(4-dimethylamino-6-{4-[5-(4-ethylpiperazin-1-ylmethyl)-4-oxo-2-phenylthiazolidin-3-yl]-phenylamino}-[1,3,5]triazin-2-yloxy)-1-methyl-1h-quinolin-2-one have been synthesized from the key intermediate 4-[4-(4-aminophenylamino)-6-dimethylamino-[1,3,5]triazin-2-yloxy]-1-methyl-1h-quinolin-2-one (7). condensation reaction of compound 7 with different aldehyde derivatives were performed to obtain schiff base derivatives, which after cyclization gav ... | 2011 | 22182927 |
| diarrhea associated with shigella in children and susceptibility to antimicrobials. | objective: to evaluate the distribution and susceptibility to antimicrobials of shigella isolated from children with acute diarrhea and without diarrhea in teresina, state of piauí, brazil. methods: four hundred children aged up to 60 months were studied. stools were collected from all the patients between january 2004 and august 2007. shigella was identified by conventional methods and antibiogram and extended-spectrum beta-lactamase (esbl) were performed by agar diffusion. results: shigellosis ... | 2011 | 22089139 |
| an inverse and independent association between helicobacter pylori infection and the incidence of shigellosis and other diarrheal diseases. | objectives. we examined the association between helicobacter pylori infection and the incidence of diarrheal diseases.methods. in a nested case-control study participants were sampled from cohorts of male israeli soldiers aged 18-21 years, serving in field units and followed up for diarrheal diseases. case patients (n = 177) were subjects who visited the base clinic because diarrhea and were positive for shigella sonnei (n = 66), enterotoxigenic escherichia coli (etec) (n = 31) or negative for b ... | 2011 | 22157171 |
| antimicrobial and toxicological activities of five medicinal plant species from cameroon traditional medicine. | infectious diseases caused by multiresistant microbial strains are on the increase. fighting these diseases with natural products may be more efficacious. the aim of this study was to investigate the in vitro antimicrobial activity of methanolic, ethylacetate (etoac) and hexanic fractions of five cameroonian medicinal plants (piptadeniastum africana, cissus aralioides, hileria latifolia, phyllanthus muellerianus and gladiolus gregasius) against 10 pathogenic microorganisms of the urogenital and ... | 2011 | 21867554 |
| microbiological quality of drinking water from dispensers in roadside restaurants of bangladesh. | the microbiological status of water from dispensers in different roadside restaurants of dhaka city and savar area was analyzed in this study. seven samples from dhaka and 8 samples of savar were checked. the heterotrophic plate count was in a range of 1.0 x 10(3) cfu ml(-1) to 2.0 x 10(4) cfu ml(-1) (from new bottles), 1.0 x 10(3) to 1.5 x 10(4) cfu ml(-1) (after dispensation), and 1.5 x 10(3) cfu ml(-1) to 1.0 x l0(5) cfu ml(-1) (from serving glass). in several of the samples, the heterotrophi ... | 2011 | 21916267 |
| validation of the h2s method to detect bacteria of fecal origin by cultured and molecular methods. | using biochemical and molecular methods, this research determined whether or not the h(2)s test did correctly identify sewage-contaminated waters by being the first to use culturing and molecular methods to identify the types and numbers of fecal indicator organisms, pathogens, and other microbes present in sewage samples with positive h(2)s test results. for the culture-based method, samples were analyzed for the presence of fecal bacteria by spread plating the sewage sample onto differential a ... | 2011 | 22038242 |
| antibacterial characteristics and activity of water-soluble chitosan derivatives prepared by the maillard reaction. | the antibacterial activity of water-soluble chitosan derivatives prepared by maillard reactions against staphylococcus aureus, listeria monocytogenes, bacillus cereus, escherichia coli, shigella dysenteriae, and salmonella typhimurium was examined. relatively high antibacterial activity against various microorganisms was noted for the chitosan-glucosamine derivative as compared to the acid-soluble chitosan. in addition, it was found that the susceptibility of the test organisms to the water-solu ... | 2011 | 21989311 |
| toxic megacolon. | toxic megacolon represents a dreaded complication of mainly inflammatory or infectious conditions of the colon. it is most commonly associated with inflammatory bowel disease (ibd), i.e., ulcerative colitis or ileocolonic crohn's disease. lately, the epidemiology has shifted toward infectious causes, specifically due to an increase of clostridium difficile-associated colitis possibly due to the extensive (ab)use of broad-spectrum antibiotics. other important infectious etiologies include salmone ... | 2011 | 22009735 |
| the effect of the potential phoq histidine kinase inhibitors on shigella flexneri virulence. | phoq/phop is an important two-component system that regulates shigella virulence. we explored whether the phoq/phop system is a promising target for new antibiotics against s. flexneri infection. by using a high-throughput screen and enzymatic activity coupled assay, four compounds were found as potential phoq inhibitors. these compounds not only inhibited the activity of sf-phoqc autophosphorylation but also displayed high binding affinities to the sf-phoqc protein in the surface plasmon resona ... | 2011 | 21853073 |
| crosslinking of (cytosine-5)-dna methyltransferase ssoii and its complexes with specific dna duplexes provides an insight into their structures. | (cytosine-5)-dna methyltransferase ssoii (m.ssoii) functions as a methyltransferase and also as a transcription factor. chemical and photochemical crosslinking was used for exploring the structure of m.ssoii-dna complexes and m.ssoii in the absence of dna. photocrosslinking with 4-(n-maleimido)benzophenone demonstrated that in the m.ssoii complex with dna containing the regulatory site, the m.ssoii region responsible for methylation was bound to dna flanking the regulatory site, which contained ... | 2011 | 21888553 |
| salmonella typhimurium-specific bacteriophage φsh19 and the origins of species specificity in the vi01-like phage family. | whole genome sequencing of bacteriophages suitable for biocontrol of pathogens in food products is a pre-requisite to any phage-based intervention procedure. trials involving the biosanitization of salmonella typhimurium in the pig production environment identified one such candidate, φsh19. | 2011 | 22047448 |
| real-time multiplex pcr for simultaneous detection of campylobacter jejuni, salmonella, shigella and yersinia species in fecal samples. | diarrheal diseases due to notifiable bacterial infections require rapid diagnosis of the causative pathogens. to facilitate detection, a real-time multiplex pcr was developed that identifies common diarrhea-causing bacteria in fecal samples. on the basis of published sequence data, sets of primers and probes were designed that were specific for campylobacter jejuni, salmonella, shigella/enteroinvasive escherichia coli eiec, and yersinia species, suitable for use in a one-tube pcr assay. the assa ... | 2011 | 21855409 |
| global distribution of shigella sonnei clones. | to investigate global epidemiology of shigella sonnei, we performed multilocus variable number tandem repeat analysis of 1,672 isolates obtained since 1943 from 50 countries on 5 continents and the pacific region. three major clonal groups were identified; 2 were globally spread. type 18 and its derivatives have circulated worldwide in recent decades. | 2011 | 22000369 |
| intestinal parasitosis and shigellosis among diarrheal patients in gondar teaching hospital, northwest ethiopia. | abstract: | 2011 | 22041102 |
| antibacterial and anti-inflammatory activity of traditional chinese herb pairs, angelica sinensis and sophora flavescens. | the purpose of the present study was to investigate the antibacterial and anti-inflammatory activity of angelica sinensis extract (ae), sophora flavescens extract (se), and herb pair a. sinensis and s. flavescens extract (hpe). endotoxin-induced uveitis (eiu) was induced in rats by a footpad injection of lipopolysaccharide. the anti-inflammatory potential of ae, se, and hpe in the regulation of nuclear factor kappa b (nf-κb), maleic dialdehyde (mda), polymorphonuclear cells (pmn), interleukin-1β ... | 2011 | 21976127 |
| waterlogsy nmr experiments in conjunction with molecular-dynamics simulations identify immobilized water molecules that bridge peptide mimic mdwnmhaa to anticarbohydrate antibody sya/j6. | x-ray crystallographic data of the carbohydrate mimic mdwnmhaa when bound to an anti-shigella flexneri y mab sya/j6 indicate the immobilization of water molecules, that is, the presence of "bound" waters, in the active site. water ligand observed via gradient spectroscopy (waterlogsy) was used in conjunction with saturation transfer difference (std)-nmr spectroscopy to probe the existence of immobilized water molecules in the complex of mdwnmhaa 1 bound to mab sya/j6. molecular dynamics simulati ... | 2011 | 21887835 |
| [etiological surveillance and analysis of infectious diarrhea in beijing in year 2010]. | to explore the pathogenic form, epidemic features and serotype distribution of the pathogenic bacteria causing infectious diarrhea in beijing. | 2011 | 22177306 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of bacteria and yeasts in a clinical microbiological laboratory: a review. | recently, maldi-tof ms devices designed for use in clinical laboratories have been commercially introduced in various large centres worldwide. all published studies conclude that maldi-tof ms can be implemented easily for routine identification of bacteria and yeasts in a clinical microbiological laboratory. although all data show that maldi-tof ms correctly identifies the great majority of isolates processed routinely, it cannot yet identify every such isolate. until today, maldi-tof ms is inap ... | 2011 | 21938981 |
| structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers. | despite a long-suspected role in the development of human colorectal cancer (crc), the composition of gut microbiota in crc patients has not been adequately described. in this study, fecal bacterial diversity in crc patients (n=46) and healthy volunteers (n=56) were profiled by 454 pyrosequencing of the v3 region of the 16s ribosomal rna gene. both principal component analysis and unifrac analysis showed structural segregation between the two populations. forty-eight operational taxonomic units ... | 2011 | 21850056 |
| Atypical Shigella boydii 13 encodes virulence factors seen in attaching and effacing Escherichia coli. | Enterohemorrhagic E. coli (EHEC) is a foodborne pathogen that causes watery diarrhea and hemorrhagic colitis. In this study, we identified StcE, a secreted zinc metalloprotease that contributes to intimate adherence of EHEC to host cells, in culture supernatants of atypical Shigella boydii 13 (Shigella B13) strains. Further examination of the Shigella B13 strains revealed that this cluster of pathogens does not invade but forms pedestals on HEp-2 cells similar to EHEC and enteropathogenic E. col ... | 2011 | 22126649 |
| social-economic factors and irrational antibiotic use as reasons for antibiotic resistance of bacteria causing common childhood infections in primary healthcare. | the most prevalent childhood bacterial infections in primary healthcare are respiratory, gastrointestinal and urogenital infections. the main aim of this paper was to consider factors (socio-economic factors and irrational antibiotic use) that contribute to the development of bacterial resistance, as well as measures that resulted in a reduction of this problem. computerized search through the medline of published articles on antibiotic resistance from 1996 to 2011 in english or serbian was comp ... | 2011 | 21987082 |
| Reactor performance and bacterial pathogen removal in response to sludge retention time in a mesophilic anaerobic digester treating sewage sludge. | The effects of sludge retention time (SRT) on reactor performance and bacterial pathogen removal of sludge mesophilic anaerobic digestion (MAD) were investigated in a continuous stirred tank reactor. The average volatile solids removal remained around 20% and the biogas production rate varied from 100 to 132ml/ld. The MAD process was efficient to remove Salmonella sp. and Escherichia coli with removal efficiencies increased with SRT from 11d, 16d to 25d. However, the Shigella sp. removal was ins ... | 2011 | 22197074 |
| analysis of synonymous codon usage in shigella flexneri 2a strain 301 and other shigella and escherichia coli strains. | in this study, we analysed synonymous codon usage in shigella flexneri 2a strain 301 (sf301) and performed a comparative analysis of synonymous codon usage patterns in sf301 and other strains of shigella and escherichia coli. although there was a significant variety in codon usage bias among different sf301 genes, there was a slight but observable codon usage bias that could primarily be attributable to mutational pressure and translational selection. in addition, the relative abundance of dinuc ... | 2011 | 22112197 |
| Ciprofloxacin-resistant Shigella sonnei among men who have sex with men, Canada, 2010. | In 2010, we observed isolates with matching pulsed-field gel electrophoresis patterns from 13 cases of ciprofloxacin-resistant Shigella sonnei in Montréal. We report on the emergence of this resistance type and a study of resistance mechanisms. The investigation suggested local transmission among men who have sex with men associated with sex venues. | 2011 | 21888811 |
| characterization of a vii-like phage specific to escherichia coli o157:h7. | phage vb_ecom_cba120 (cba120), isolated against escherichia coli o157:h7 from a cattle feedlot, is morphologically very similar to the classic phage vii of salmonella enterica serovar typhi. until recently, little was known genetically or physiologically about the vii-like phages, and none targeting e. coli have been described in the literature. the genome of cba120 has been fully sequenced and is highly similar to those of both vii and the shigella phage ag3. the core set of structural and repl ... | 2011 | 21899740 |
| Further characterization of Shigella sonnei live vaccine candidates WRSs2 and WRSs3-plasmid composition, invasion assays and Sereny reactions. | 2011 | 21983066 | |
| Clinical characteristics and antibiotic resistance of Shigella gastroenteritis in Ankara, Turkey between 2003 and 2009, and comparison with previous reports. | The aim of this study was to define the epidemiological, clinical, and antibiotic susceptibility patterns of Shigella gastroenteritis cases occurring during the years 2003-2009 and to compare results with those of the years 1987-2002. | 2011 | 21982814 |
| convergent synthesis of the tetrasaccharide repeating unit of the o-antigen of shigella boydii type 9. | a convenient synthesis of the tetrasaccharide repeating unit of the o-antigen of shigella boydii type 9 has been achieved in excellent yield using a [2 + 2] block glycosylation strategy. tempo-mediated selective oxidation of the primary alcohol of the tetrasaccharide derivative 8 to the carboxylic group followed by deprotection of the functional groups furnished target tetrasaccharide 1 as its 4-methoxyphenyl glycoside in high yield. | 2011 | 21977201 |
| synthesis of novel quinoxalinone derivatives by conventional and microwave methods and assessing their biological activity. | in this study, twenty-one arylaminoquinoxalinone derivatives were synthesized and their antibacterial activities against staphylococci aureus, pseudomonas aureus, escherichia coli, bacillus subtilis, salmonella typhi, and shigella pneumoniae were evaluated relative to known antibiotics; augmentin, ampicillin, and chloramphenicol. the insecticidal activities of the prepared compounds were also investigated against tribolium castaneum using permethrin as a standard insecticide. the derivatives wer ... | 2011 | 22076760 |
| the role of oxyr and soxrs in oxidative stress survival in shigella flexneri. | shigella flexneri, a facultative intracellular pathogen, is exposed to a variety of environments inside and outside of the human host. some of these environments may contain significant oxidative stress. s. flexneri mutants were generated with deletions in the major oxidative stress regulators oxyr and/or soxrs to test their importance in shigella biology. strains that contained a deletion of oxyr had reduced growth and survival during aerobic growth, but not microaerobic growth. the mutants wer ... | 2011 | 22015257 |
| live attenuated shigella dysenteriae type 1 vaccine strains overexpressing shiga toxin b subunit. | shigella dysenteriae serotype 1 (s. dysenteriae 1) is unique among the shigella species and serotypes in the expression of shiga toxin which contributes to more severe disease sequelae and the ability to cause explosive outbreaks and pandemics. s. dysenteriae 1 shares characteristics with other shigella species, including the capability of causing clinical illness with a very low inoculum (10 to 100 cfu) and resistance to multiple antibiotics, underscoring the need for efficacious vaccines and t ... | 2011 | 21969003 |
| Comparative evaluation of the antibody in lymphocyte supernatant (ALS) and enzyme-linked immunospot (ELISPOT) assays for measuring mucosal immune responses to Shigella antigens. | Accurately assessing mucosal immune responses to candidate vaccines remains a technical challenge. ELISPOT is widely used as a surrogate of mucosal immune response by directly enumerating circulating antibody secreting cells (ASCs), while antibody in lymphocyte supernatant (ALS) titers the total amount of antibody secreted by ASC ex vivo using ELISA. ALS is more practical than ELISPOT because the ASC supernatant is frozen for ELISA that can be conducted at any time, with any antigen, and in any ... | 2011 | 21939714 |
| a school outbreak of shigella sonnei infection in china: clinical features, antibiotic susceptibility and molecular epidemiology. | objective: to describe the clinical features of infection, and the antibiotic susceptibility of epidemic strains, and investigate plasmid maps and integrons of the isolates from an outbreak of shigella sonnei infection at an elementary school in southwest china. study design: cross-sectional study. setting: an elementary school and five hospitals in chengdu in southwest china. results: there were 1,134 students in the school. 937 (82.6%) students had signs and symptoms. of the 568 (60.6%, 568/93 ... | 2011 | 21992857 |
| Evolutionary and functional insights into Leishmania META1: evidence for lateral gene transfer and a role for META1 in secretion. | ABSTRACT: BACKGROUND: Leishmania META1 has for long been a candidate molecule for involvement in virulence: META1 transcript and protein are up-regulated in metacyclic Leishmania. Yet, how META1 contributes to virulence remains unclear. We sought insights into the possible functions of META1 by studying its evolutionary origins. RESULTS: Using multiple criteria including sequence similarity, nucleotide composition, phylogenetic analysis and selection pressure on gene sequence, we present evide ... | 2011 | 22093578 |
| A new piece of the Shigella pathogenicity puzzle: spermidine accumulationby silencing of the speG gene. | The genome of Shigella, a gram negative bacterium which is the causative agent of bacillary dysentery, shares strong homologies with that of its commensal ancestor, Escherichia coli. The acquisition, by lateral gene transfer, of a large plasmid carrying virulence determinants has been a crucial event in the evolution towards the pathogenic lifestyle and has been paralleled by the occurrence of mutations affecting genes, which negatively interfere with the expression of virulence factors. In this ... | 2011 | 22102881 |
| topological characterisation and identification of critical domains within glucosyltransferase iv (gtriv) of shigella flexneri. | abstract: | 2011 | 22188643 |
| The effects of solid media type on routine identification of bacterial isolates using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. | Background: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis is a rapid method for the identification of bacteria based upon the comparison of protein profiles. Factors that may alter profiles include culture conditions and presence of exogenous substances and could hinder identification. Methods: Bacterial isolates identified using conventional methods were cultured on various media and identified using the MALDI Biotyper system (Bruker Dalton ... | 2011 | 22162546 |
| [Dysentery and amoebiasis in Poland in 2009]. | Only 30 cases were notified (incidence rate 0.08/100 000 population) in the year 2009, while the 33 cases were notified in 2008 (incidence rate 0.09/100 000), and median in the 2003-2007 years was 74 cases, incidence rate 0.19/100 000 population. Since 2000 no one-death case was notified. In the period of low frequency of Shigella infections, the external quality assessment control of the quality of bacteriological media, laboratory and prae-laboratory procedures for detection of different Shige ... | 2011 | 21913464 |
| The scope of phage display for membrane proteins. | Numerous examples of phage display applied to soluble proteins demonstrate the power of the technique for protein engineering, affinity reagent discovery and structure-function studies. Recent reports have expanded phage display to include membrane proteins (MPs). The scope and limitations of MP display remain undefined. Therefore, we report data from the phage display of representative types of membrane-associated proteins including plasma, nuclear, peripheral, single and multipass. The periphe ... | 2011 | 22037583 |
| reflectron maldi tof and maldi tof/tof mass spectrometry reveal novel structural details of native lipooligosaccharides. | lipooligosaccharides (los) are powerful gram-negative glycolipids that evade the immune system and invade host animal and vegetal cells. the structural elucidation of los is pivotal to understanding the mechanisms of infection at the molecular level. the amphiphilic nature of los has been the main obstacle for structural analysis by matrix-assisted laser desorption/ionization (maldi) mass spectrometry (ms). our approach has resolved this important issue and has permitted us to obtain reflectron ... | 2011 | 22124985 |
| Misidentification of a commensal inactive Escherichia coli as Shigella sonnei by an automated system in a critically ill patient. | Though automated identification and sensitivity testing has established itself as a standard in many high throughput clinical microbiology laboratories, misidentification by the automated systems can also happen. | 2011 | 22029193 |
| outbreak of shigella sonnei infection in norway linked to consumption of fresh basil, october 2011. | we report a shigella sonnei outbreak of 46 cases that occurred in norway during october 2011. two municipalities were involved. a large cluster (42 cases)was concentrated in north norway, while a smallcluster (4 cases) occurred in the south-east region.epidemiological evidence and trace back investigations have linked the outbreak to the consumption of imported fresh basil. the product has been withdrawn from the market. no further cases have been reported since 25 october. | 2011 | 22085618 |
| prevalence and antimicrobial susceptibility patterns of shigella species at felege hiwot referral hospital, northwest ethiopia. | shigella species infections coupled with increased levels of antimicrobial resistances are major public health problems in developing countries particularly in children. | 2011 | 21991758 |
| foodborne-associated shigella sonnei, india, 2009 and 2010. | 2011 | 22099103 | |
| evolutionary silence of the acid chaperone protein hdeb in enterohemorrhagic escherichia coli o157:h7. | the periplasmic chaperones hdea and hdeb are known to be important for cell survival at low ph (ph<3) in escherichia coli and shigella spp. here we investigated the roles of hdea and hdeb in the survival of various enterohemorrhagic e. coli (ehec) following exposure to ph 2.0. similar to k-12 strains, the acid protections conferred by hdea and hdeb in ehec o145 were significant: loss of hdea and hdeb led to over 100- to 1000-fold reductions in acid survival, depending on the growth condition of ... | 2011 | 22179243 |
| rational design of an artificial genetic switch: co-option of the h-ns-repressed prou operon by the virb virulence master regulator. | the h-ns protein represses the transcription of hundreds of genes in gram-negative bacteria. derepression is achieved by a multitude of mechanisms, many of which involve the binding of a protein to dna at the repressed promoter in a manner that compromises the maintenance of the h-ns-dna nucleoprotein repression complex. the principal virulence gene promoters in shigella flexneri, the cause of bacillary dysentery, are repressed by h-ns. virb, a protein that closely resembles members of the parb ... | 2011 | 21873493 |
| Crystal Structure of the VapBC Toxin-Antitoxin Complex from Shigella flexneri Reveals a Hetero-Octameric DNA-Binding Assembly. | Toxin-antitoxin (TA) loci are common in archaea and prokaryotes and allow cells to rapidly adapt to changing environmental conditions through release of active regulators of metabolism. Many toxins are endonucleases that target cellular mRNA and tRNAs, while the antitoxins tightly wrap around the toxins to inhibit them under normal circumstances. The antitoxins also bind to operators in the promoter regions of the cognate TA operon and thereby regulate transcription. For enteric vapBC TA loci, t ... | 2011 | 22037005 |
| applications of pcr (real-time and masstag) and enzyme-linked immunosorbent assay in diagnosis of respiratory infections and diarrheal illness among deployed u.s. military personnel during exercise balikatan 2009, philippines. | laboratory-based surveillance for diarrheal and respiratory illness was conducted at the 2009 republic of the philippines-united states balikatan exercise to determine the presence of specific pathogens endemic in the locations where the military exercises were conducted. ten stool and 6 respiratory specimens were obtained from individuals meeting case definitions for diarrhea or respiratory illness. stool specimens were frozen in dry ice and remotely tested using enzyme-linked immunosorbent ass ... | 2011 | 22128641 |
| the inhibitory activity of lactobacillus spp. isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin. | milk acts as a mean for transporting many essential substances from the mother to the child. in human beings, milk includes several predominant bacteria, such as staphylococci, streptococci, micrococci, lactobacilli, enterococci, lactococci and bifidobacteria. besides, its intake favors the predominance of bifidobacteria and lactobacilli in the child's intestinal microbiota. the present work explores the isolation and selection of lactobacilli strains with probiotic potential, focusing in their ... | 2011 | 21846506 |
| interaction of the salmonella typhimurium effector protein sopb with host cell cdc42 is involved in intracellular replication. | the phosphoinositide phosphatase sopb/sigd is a type iii secretion system effector that plays multiple roles in salmonella internalization and intracellular survival. we previously reported that sopb complexed with and inhibited the small gtpase cdc42 when expressed in a yeast model system, independently of its phosphatase activity. here we show that human cdc42, but not rac1, interacts with catalytically inactive sopb when coexpressed in saccharomyces cerevisiae. this interaction occurs with bo ... | 2011 | 21435037 |
| multi-drug resistance and characteristic of integrons in shigella spp. isolated from china. | to investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in shigella spp. | 2011 | 21440840 |
| quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad. | stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative pcr (qpcr). stx phages from the samples were propagated in escherichia coli c600, e. coli o157:h7, and shigella strains and further quantified. fifty percent of the samples carried infectious stx phages that were isolated from plaques generated by lysis. | 2011 | 21441341 |
| antimicrobial susceptibilities of enteric bacterial pathogens isolated in kathmandu, nepal, during 2002-2004. | the prevalence and antimicrobial susceptibility patterns of the bacterial enteropathogens vibrio cholerae, salmonella species and shigella species were investigated. | 2011 | 21444984 |
| Detection of diarrhoeal pathogens in human faeces using an automated, robotic platform. | Infectious diarrhoeal diseases represent a major socio-economic burden to humans, and are linked to a range of pathogens, including viruses, bacteria and protists. The accurate detection of such pathogens is central to control. However, detection often relies on methods that have limited diagnostic sensitivity and specificity. Here, we assessed an automated, robotic platform for the simultaneous detection of eight major pathogens associated with infectious diarrhoea. Genomic DNA samples (n = 167 ... | 2011 | 22056326 |
| Genotyping of selected bacterial enteropathogens in Norway. | In Norway the Norwegian Institute of Public Health (NIPH) is the primary facility for nationwide surveillance of foodborne infections, and it is vital that we can perform rapid and high resolution identification of foodborne bacteria at the strain level. During the last decade a rapid introduction of DNA-based methods has been introduced, which show promise in enhancing the speed and discriminatory capability of the typing laboratory. The laboratory responsible for genotyping enteropathogens at ... | 2011 | 21975141 |
| Third-generation cephalosporin resistance in clinical isolate of Shigella sonnei in Andaman & Nicobar Islands, India. | 2011 | 21918310 | |
| Differential host immune responses to epidemic and endemic strains of Shigella dysenteriae type I. | Shigella dysenteriae type 1 causes devastating epidemics in developing countries with high case-fatality rates in all age-groups. The aim of the study was to compare host immune responses to epidemic (T2218) and endemic strains of S. dysenteriae type 1. Shigellacidal activity of serum from rabbits immunized with epidemic or endemic strains, S. dysenteriae type 1-infected patients, and healthy adult controls from Shigella-endemic and non-endemic regions was measured. Immunogenic cross-reactivity ... | 2011 | 22106748 |
| Natural Products Modulate Shigella-Host Cell Interaction. | This study focussed on identifying possible new options derived from natural sources for the treatment of bacterial infections. Several natural products were investigated for their potential in modulating Shigella-host cell interaction. The proliferation of Shigella sonnei was effectively inhibited inside HEp-2 cells in the presence of 4-methoxycinnamic acid and propolin D. Propolin D also significantly reduced the apopotosis of infected macrophage-like U937 cells, and moderately reduced the sec ... | 2011 | 21719574 |
| antisense inhibition of gene expression and growth in gram-negative bacteria by cell-penetrating peptide conjugates of peptide nucleic acids targeted to rpod gene. | gram-negative bacteria (gnb) cause common and severe hospital- and community-acquired infections with a high incidence of multidrug resistance (mdr) and mortality. the emergence and spread of mdr-gnb strains limit therapeutic options and highlight the need to develop new therapeutic strategies. in this study, the peptide (rxr)(4)xb- and (kff)(3)k-conjugated peptide nucleic acids (ppnas) were developed to target rpod, which encodes an rna polymerase primary σ(70) that is thought to be essential f ... | 2011 | 22000398 |
| bacteriological assessment of drinking water supply options in coastal areas of bangladesh. | this study was conducted to assess the bacteriological quality of alternative drinking water supply options in southwest coastal areas of bangladesh. a total of 90 water samples were collected during both dry and wet seasons from household based rainwater harvesting systems (rwhss), community based rain water harvesting systems (crwhss), pond-sand filters (psfs) and ponds. the samples were evaluated for faecal coliform, escherichia coli and heterotrophic plate count, as well as vibrio cholerae, ... | 2011 | 21942205 |
| gene decay in shigella as an incipient stage of host-adaptation. | background: many facultative bacterial pathogens have undergone extensive gene decay processes, possibly due to lack of selection pressure during evolutionary conversion from free-living to intracellular lifestyle. shigella, the causative agents of human shigellosis, have arisen from different e. coli-like ancestors independently by convergent paths. as these bacteria all have lost large numbers of genes by mutation or deletion, they can be used as ideal models for systematically studying the pr ... | 2011 | 22110755 |
| chemical and antidiarrheal studies of plinia cauliflora. | plinia cauliflora (mart.) kausel, widespread in south america, has edible fruits, and its bark is commonly used against diarrhea and other disorders, on account of its astringency. because diarrhea is still one of the most important causes of illness and death among children in developing countries, where the population turns to traditional medicine for its treatment, the present study determined the composition of fruit and leaf extracts of p. cauliflora, analyzed the activity against diarrhea ... | 2011 | 21861716 |
| Within-species lateral genetic transfer and the evolution of transcriptional regulation in Escherichia coli and Shigella. | Changes in transcriptional regulation underlie many of the phenotypic differences observed within and between species of bacteria. Lateral genetic transfer (LGT) can significantly impact the transcription factor (TF) genes which drive these transcriptional changes. Although much emphasis has been placed on LGT of intact genes, the units of transfer and recombination do not necessarily correspond to regions delineated by exact gene boundaries. Here we apply phylogenetic and network-based methods ... | 2011 | 22035052 |
| Differentiation between Shigella, enteroinvasive Escherichia coli (EIEC) and noninvasive Escherichia coli. | Shigella causes bacillary dysentery and is classified into four species based on their antigen characteristics. This classification does not reflect genetic relatedness; in fact, Shigella species are so related to Escherichia coli , they should be classified as one distinctive species in the genus Escherichia. The differentiation of Shigella and E. coli is even more complicated with the description of enteroinvasive E. coli (EIEC). EIEC are strains that possess some of the biochemical characteri ... | 2011 | 21901636 |
| Genesis of a novel Shigella flexneri serotype by sequential infection of serotype-converting bacteriophages SfX and SfI. | ABSTRACT: BACKGROUND: Shigella flexneri is the major pathogen causing bacillary dysentery. Fifteen serotypes have been recognized up to now. The genesis of new S. flexneri serotypes is commonly mediated by serotype-converting bacteriophages. Untypeable or novel serotypes from natural infections had been reported worldwide but have not been generated in laboratory. RESULTS: A new S. flexneri serotype-serotype 1 d was generated when a S. flexneri serotype Y strain (native LPS) was sequentially in ... | 2011 | 22208551 |
| [Investigation of verotoxigenic escherichia coli O157:H7 incidence in gastroenteritis patients]. | Escherichia coli O157:H7 is the most common serotype among verotoxigenic E.coli (VTEC) strains that cause haemolytic uremic syndrome. Although sporadic VTEC cases originating from Turkey and small outbreaks have been reported from our country, VTEC has not been routinely investigated in most of the diagnostic microbiology laboratories in Turkey and studies related to this topic are limited. In this study, the incidence of E.coli O157:H7 in patients who were admitted to Alanya Research and Applic ... | 2011 | 21935785 |
| Mucosal immunization with Shigella flexneri outer membrane vesicles induced protection in mice. | Vaccination appears to be the only rational prophylactic approach to control shigellosis. Unfortunately, there is still no safe and efficacious vaccine available. We investigated the protection conferred by a new vaccine containing outer membrane vesicles (OMVs) from Shigella flexneri with an adjuvant based on nanoparticles in an experimental model of shigellosis in mice. OMVs were encapsulated in poly(anhydride) nanoparticles prepared by a solvent displacement method with the copolymer PMV/MA. ... | 2011 | 21911022 |
| Gut microbiota and pediatric disease. | Background: Researchers have made every effort to assess the role of gut microbiota in pediatric diseases like inflammatory bowel disease (IBD), celiac disease, asthma, allergy, and autism. The leading hypothesis is that an altered microbial composition is present (other than the presence of a specific pathogen) and that it could be involved in the pathogenesis or progression of such disorders. Methods: Cultural, molecular, metabolomic, and metagenomic approaches are trying to define the pediatr ... | 2011 | 22179208 |
| whole-genome phylogeny of escherichia coli/shigella group by feature frequency profiles (ffps). | a whole-genome phylogeny of the escherichia coli/shigella group was constructed by using the feature frequency profile (ffp) method. this alignment-free approach uses the frequencies of l-mer features of whole genomes to infer phylogenic distances. we present two phylogenies that accentuate different aspects of e. coli/shigella genomic evolution: (i) one based on the compositions of all possible features of length l = 24 (∼8.4 million features), which are likely to reveal the phenetic grouping a ... | 2011 | 21536867 |
| rapid situation & response assessment of diarrhoea outbreak in a coastal district following tropical cyclone aila in india. | background & objectives : cyclone aila hit indian states on eastern coast on may 25, 2009. an investigation was conducted to examine if aila was responsible for increased reporting of diarrhoea cases from the district of east-medinipur in west bengal. identifying causative organisms for diarrhoea and assessing their antibiotic susceptibility profile were other objectives. methods: rapid situation and response assessment technique was employed to triangulate primary and secondary data collected t ... | 2011 | 21537092 |
| toxic megacolon from sexually transmitted shigella sonnei infection. | 2011 | 21538050 |