Publications

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growth inhibitory effect of phenolic extracts of ziziphus jujuba mill. in dengue vector aedes aegypti (l) in parent and f1 generation.to evaluate the lethal and emergence inhibitory effect of alkaloid, phenolic and terpenoid extracts of ziziphus jujuba (rhamnaceae) against aedes aegypti (diptera: culicidae), and to explore the effect of the most effective fraction on developmental and biochemical parameters of the dengue vector.201728942827
chemical composition of buddleja polystachya aerial parts and its bioactivity against aedes aegypti.a new acylatediridoid glycoside, 6-o-α-l-(2″-acetyl-4″-o-trans-isoferuloyl) rhamnopyranosyl catalpol (9) together with 18 known compounds belonging to the iridoids, flavonoids, triterpene saponin glycosides and phenylethanoids (1-8, 10-18) were isolated from the aerial parts and the flowers of buddleja polystachya. their structures were elucidated on the basis of spectroscopic evidence and comparison with that reported in the literature. promising adulticidal activity was shown for all extracts ...201728942684
insights into the epidemiological link between biting flies and pemphigus foliaceus in southeastern brazil.black fly and sandfly bites are related to the endemicity of pemphigus foliaceus (pf); however, an immune reaction against the salivary proteins from these flies still requires confirmation in the case of pf patients living in southeastern brazil.201728941730
contribution of lysinibacillus sphaericus hemolysin and chitin-binding protein in entomopathogenic activity against insecticide resistant aedes aegypti.given its toxicity against culicid larvae, lysinibacillus sphaericus is used for the biological control of mosquitoes such as culex sp. and anopheles sp. the toxicity factors currently reported for l. sphaericus include the binary toxin, mtx toxins, and the s-layer. however, aedes aegypti is refractory to the binary toxin, the most toxic larvicidal protein of l. sphaericus. until now, there are no evidences of the hemolytic and chitinolytic capacity of l. sphaericus. herein, the expression of th ...201728940156
molecular typing and phylogeny of wolbachia: a study from assam, north-eastern part of india.wolbachia are maternally inherited endosymbiotic alphaproteobacteria, infecting 40-75% of arthropod species. knowledge on distribution of native strains infecting mosquito vectors from endemic regions is essential for successful implementation of vector control interventions utilizing potential strains of wolbachia. study identified various native strains of wolbachia inhabiting different mosquito species from field and colonised conditions of assam. the fly drosophila melanogaster was also incl ...201728939497
the dengue preface to endemic in mainland china: the historical largest outbreak by aedes albopictus in guangzhou, 2014.dengue was regarded as a mild epidemic in mainland china transmitted by aedes albopictus. however, the 2014 record-breaking outbreak in guangzhou could change the situation. in order to provide an early warning of epidemic trends and provide evidence for prevention and control strategies, we seek to characterize the 2014 outbreak through application of detailed cases and entomological data, as well as phylogenetic analysis of viral envelope (e) gene.201728934991
isolation and identification of xenorhabdus and photorhabdus bacteria associated with entomopathogenic nematodes and their larvicidal activity against aedes aegypti.aedes aegypti is a potential vector of west nile, japanese encephalitis, chikungunya, dengue and zika viruses. alternative control measurements of the vector are needed to overcome the problems of environmental contamination and chemical resistance. xenorhabdus and photorhabdus are symbionts in the intestine of entomopathogenic nematodes (epns) steinernema spp. and heterorhabditis spp. these bacteria are able to produce a broad range of bioactive compounds including antimicrobial, antiparasitic, ...201728934970
proteomics technique opens new frontiers in mobilome research.a large proportion of the genome of most eukaryotic organisms consists of highly repetitive mobile genetic elements. the sum of these elements is called the "mobilome," which in eukaryotes is made up mostly of transposons. transposable elements contribute to disease, evolution, and normal physiology by mediating genetic rearrangement, and through the "domestication" of transposon proteins for cellular functions. although 'omics studies of mobilome genomes and transcriptomes are common, technical ...201728932623
zika virus congenital syndrome: experimental models and clinical aspects.viral infections have long been the cause of severe diseases to humans, increasing morbidity and mortality rates worldwide, either in rich or poor countries. yellow fever virus, h1n1 virus, hiv, dengue virus, hepatitis b and c are well known threats to human health, being responsible for many million deaths annually, associated to a huge economic and social cost. in this context, a recently introduced flavivirus in south america, called zika virus (zikv), led the who to declare in february 1st 2 ...201728932235
ph control in the midgut of aedesaegypti under different nutritional conditions.aedes aegypti is one of the most important disease vectors in the world. because their gut is the first site of interaction with pathogens, it is important to understand a. aegypti gut physiology. in this study, we investigated the mechanisms of ph control in the midgut of a. aegypti females under different nutritional conditions. we found that unfed females have an acidic midgut (ph ∼6). the midgut of unfed insects is actively maintained at ph 6 regardless of the ingestion of either alkaline or ...201728931720
microgeographic population structuring of aedes aegypti (diptera: culicidae).aedes aegypti is one of the species most favored by changes in the environment caused by urbanization. its abundance increases rapidly in the face of such changes, increasing the risk of disease transmission. previous studies have shown that mosquito species that have adapted to anthropogenic environmental changes benefit from urbanization and undergo population expansion. in light of this, we used microsatellite markers to explore how urbanization processes may be modulating ae. aegypti populat ...201728931078
pyrethroid resistance alters the blood-feeding behavior in puerto rican aedes aegypti mosquitoes exposed to treated fabric.emerging insecticide resistance is a major issue for vector control. it decreases the effectiveness of insecticides, thereby requiring greater quantities for comparable control with a net increase in risk of disease resurgence, product cost, and damage risk to the ecosystem. pyrethroid resistance has been documented in puerto rican populations of aedes aegypti (l.) mosquitoes. in this study, topical toxicity of five insecticides (permethrin, etofenprox, deltamethrin, ddt, transfluthrin) was dete ...201728931018
emerging arboviruses in rio grande do sul, brazil: chikungunya and zika outbreaks, 2014-2016.the recent emergence of arboviruses such as chikungunya virus (chikv) and zika virus (zikv) in brazil has posed a threat to human health and to the country's economy. outbreaks occur mainly in tropical areas; however, increasing number of cases have been observed in rio grande do sul (rs), the southernmost state; therefore, surveillance of these arboviruses is essential for public health measures.201728929534
sybr green-based one step quantitative real-time polymerase chain reaction assay for the detection of zika virus in field-caught mosquitoes.the monitoring of vectors is one of the key surveillance measures to assess the risk of arbovirus transmission and the success of control strategies in endemic regions. the recent re-emergence of zika virus (zikv) in the tropics, including singapore, emphasizes the need to develop cost-effective, rapid and accurate assays to monitor the virus spread by mosquitoes. as zikv infections largely remain asymptomatic, early detection of zikv in the field-caught mosquitoes enables timely implementation ...201728927458
insecticide susceptibility of aedes albopictus and ae. aegypti from brazil and the swiss-italian border region.aedes aegypti and ae. albopictus are two highly invasive mosquito species, both vectors of several viruses, including dengue, chikungunya and zika. while ae. aegypti is the primary vector in the tropics and sub-tropics, ae. albopictus is increasingly under the public health watch as it has been implicated in arbovirus-transmission in more temperate regions, including continental europe. vector control using insecticides is the pillar of most control programmes; hence development of insecticide r ...201728927441
community participation in mosquito breeding site control: an interdisciplinary mixed methods study in curaçao.as the arboviral diseases dengue, chikungunya and zika emerge in the americas, so does the need for sustainable vector control policies. to successfully achieve mosquito control, joint efforts of both communities and governments are essential. this study investigates this important, but by-and-large neglected topic.201728927437
differential expression of putative sodium-dependent cation-chloride cotransporters in aedes aegypti.the yellow fever mosquito, aedes aegypti, has three genes that code for proteins with sequence similarity to vertebrate na+-k+-cl- cotransporters (nkccs) of the solute-linked carrier 12 superfamily of cation-chloride cotransporters (cccs). we hypothesized that these mosquito nkcc orthologues have diverged to perform distinct roles in salt secretion and absorption. in phylogenetic analyses, one protein (aenkcc1) groups with a drosophila melanogaster nkcc that mediates salt secretion whereas two o ...201728923771
role of the venus kinase receptor in the female reproductive physiology of the desert locust, schistocerca gregaria.venus kinase receptors (vkr) are a subfamily of invertebrate receptor tyrosine kinases, which have only recently been discovered. they contain an intracellular tyrosine kinase domain and an extracellular venus flytrap domain. vkrs have been functionally and pharmacologically characterized in only two invertebrate species, namely the human parasite schistosoma mansoni and the mosquito aedes aegypti, where they play a crucial role in oogenesis. here, we report the characterization of a vkr in the ...201728916758
rapid evolution of ovarian-biased genes in the yellow fever mosquito (aedes aegypti).males and females exhibit highly dimorphic phenotypes, particularly in their gonads, which is believed to be driven largely by differential gene expression. typically, the protein sequences of genes upregulated in males, or male-biased genes, evolve rapidly as compared to female-biased and unbiased genes. to date, the specific study of gonad-biased genes remains uncommon in metazoans. here, we identified and studied a total of 2927, 2013, and 4449 coding sequences (cds) with ovary-biased, testis ...201728630112
determinants of outbreaks of emerging infectious diseases : the case of chikungunya in indian ocean, 2004-2007.emerging outbreaks of arboviroses are most often tied to the convergence of a set of factors which have physical and environmental, genetic and biological, ecological, social, political and economic origins. thirteen out of these factors are known as main determinants of emerging infectious disease outbreaks. this paper assesses the role of these factors from exploring the chikungunya outbreak which occurred in indian ocean in 2004-2007, as case study. chikungunya is an alphavirus borne and tran ...201728627853
the genetic diversity and population structure of domestic aedes aegypti (diptera: culicidae) in yunnan province, southwestern china.there was no record of aedes aegypti in yunnan province, china, until 2002, but this species is now continuously found in nine cities (or counties). until now, little was known about the genetic diversity and population structure of this invasive species. thus, a detailed understanding of the invasion strategies, colonisation and dispersal of this mosquito from a population genetics perspective is urgently needed for controlling and eliminating this disease vector.201728610594
deltamethrin resistance in aedes aegypti results in treatment failure in merida, mexico.the operational impact of deltamethrin resistance on the efficacy of indoor insecticide applications to control aedes aegypti was evaluated in merida, mexico. a randomized controlled trial quantified the efficacy of indoor residual spraying (irs) against adult ae. aegypti in houses treated with either deltamethrin (to which local ae. aegypti expressed a high degree of resistance) or bendiocarb (to which local ae. aegypti were fully susceptible) as compared to untreated control houses. all adult ...201728604781
qsar models for predicting the toxicity of piperidine derivatives against aedes aegypti.qsar models are proposed for predicting the toxicity of 33 piperidine derivatives against aedes aegypti. from 2d topological descriptors, calculated with the padel software, ordinary least squares multilinear regression (ols-mlr) treatment from the qsarins software and machine learning and related approaches including linear and radial support vector machine (svm), projection pursuit regression (ppr), radial basis function neural network (rbfnn), general regression neural network (grnn) and k-ne ...201728604113
[new strategies for the eradication of aedes aegypti: what challenges do we face in latin america?]not available.201728603242
comparative efficiency of biogents gravid aedes trap, cdc autocidal gravid ovitrap, and cdc gravid trap in northeastern florida.we conducted a study to compare the effectiveness of the biogents gravid aedes trap (bg-gat) and centers for disease control and prevention (cdc) autocidal gravid ovitrap (ago) with that of the cdc gravid trap (cdc-gt) (as a standard) for their proficiency to collect mosquitoes in an urban residential neighborhood in northeastern florida. aedes aegypti , ae. albopictus, and culex quinquefasciatus were collected from each trap, with the latter species being predominant. significantly more cx. qui ...201728590226
comparison of mosquito abundance from biogents sentinel 2.0 traps with and without rain covers.biogents sentinel (bgs) traps have rapidly become a standard for adult aedes aegypti surveillance. several investigators have found that trap collections can be damaged easily by heavy dew or rain entering the trap intake port. in addition, water entering the trap may temporarily stop the fan, thus reducing the collection potential of the trap. we evaluated the effectiveness of a rain cover designed to minimize damage to mosquito trap collections from bgs 2.0 traps in a residential backyard in j ...201728590218
diagnostic doses of insecticides for adult aedes aegypti to assess insecticide resistance in cuba.the objective of this study was to determine diagnostic doses (dds) of 5 insecticides for the rockefeller susceptible strain of aedes aegypti , using the centers for disease control and prevention (cdc) bottle bioassay as a tool for monitoring insecticide resistance in the cuban vector control program. the 30-min dd values determined in this study were 13.5 μg/ml, 6.5 μg/ml, 6 μg/ml, 90.0 μg/ml, and 15.0 μg/ml for cypermethrin, deltamethrin, lambda-cyhalothrin, chlorpyrifos, and propoxur, respec ...201728590216
effects of low-powered rf sweep between 0.01-20 ghz on female aedes aegypti mosquitoes: a collective behaviour analysis.there are many products claiming to be an electronic solution towards repelling mosquitoes. several reviews were published in debunking these claims. however, there is a lack of a systematic study on effects of electromagnetic (em) or more specifically, radio frequency (rf) waves against mosquitoes due to the conclusions made in those years. therefore, we attempt to establish a fundamental study on female aedes aegypti (linnaeus) mosquitoes by quantifying the collective behavior of the mosquitoe ...201728582398
corrigendum to "functional and immunohistochemical characterization of cceae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors aedes aegypti and ae. albopictus" [insect biochem. mol. biol. 74 (july 2016) 61-67]. 201728578775
detection and perception of generic host volatiles by mosquitoes: responses to co2 constrains host-seeking behaviour.natural selection has favoured specialization in anthropophilic mosquito host choice, yet in the absence of human hosts, females feed on a selected range of vertebrates. for host recognition, we hypothesize that mosquitoes primarily rely on generic host volatiles. detection and perception of such compounds would provide the mosquito with a flexible, yet constrained, odour coding system that could delineate host preference. in this study, we show that the quintessential generic volatile for host- ...201728573028
a potent larvicidal agent against aedes aegypti mosquito from cardanol.cardanol is a constituent of cashew nut shell liquid that presents larvicidal activity against aedes aegypti. the isolation of cardanol is somewhat troublesome, however, in this work we describe an efficient and inexpensive method to obtain it as a pure material. the compound was used as starting material to make chemical transformation leading to saturated cardanol, epoxides and, halohydrins. these derivatives were tested for toxicity against aedes aegypti larvae. the results showed that iodohy ...201728562821
a new in vitro bioassay system for the discovery and quantitative evaluation of mosquito repellents.mosquitoes vector many pathogens that cause human diseases. repellents play a significant role in reducing the risk of these diseases by preventing mosquito bites. in this paper, we are reporting an ali and khan (a & k), large cage in vitro bioassay system that can effectively be used to measure repellency of compounds against mosquitoes. the system uses temperature as a landing and feeding stimulus, and collagen as a feeding substrate. the minimum effective dose (med) of deet (n, n-diethyl-meta ...201728541424
rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function.introduction: the life history of aedes aegypti presents diverse challenges to its diuretic system. during the larval and pupal life stages mosquitoes are aquatic. with the emergence of the adult they become terrestrial. this shifts the organism within minutes from an aquatic environment to a terrestrial environment where dehydration has to be avoided. in addition, female mosquitoes take large blood meals, which present an entirely new set of challenges to salt and water homeostasis. methods: to ...201728536536
 introducción. el virus del zika (zikv) es un flavivirus con envoltura, transmitido a los seres humanos principalmente por el vector aedes aegypti. la infección por zikv se ha asociado con un gran neurotropismo y con efectos neuropáticos, como el síndrome de guillain-barré en el adulto y la microcefalia fetal y posnatal, así como con un síndrome de infección congénita similar al producido por el virus de la rubéola (rv).objetivo. comparar las estructuras moleculares de la proteína de envoltura e ...201728527274
quantitative structure-activity relationship study of amide mosquito repellents.a quantitative structure-activity relationship (qsar) study on 43 amide repellents was carried out by the heuristic method in order to reveal the correlations between molecular parameters of these amides and their repellency against aedes aegypti. sketches and optimizations of molecular structures were achieved by the gaussian software package. generation and screening of molecular parameters were accomplished using codessa 2.7.10 software. the leave-one-out method was applied for the model vali ...201728521600
heat in the southeastern united states: characteristics, trends, and potential health impact.high summer temperatures in extratropical areas have an impact on the public's health, mainly through heat stress, high air pollution concentrations, and the transmission of tropical diseases. the purpose of this study is to examine the current characteristics of heat events and future projections of summer apparent temperature (at)-and associated health concerns-throughout the southeastern united states. synoptic climatology was used to assess the atmospheric characteristics of extreme heat day ...201728520817
laboratory and semi-field evaluations of two (transfluthrin) spatial repellent devices against aedes aegypti (l.) (diptera: culicidae).two transfluthrin-based spatial repellent products (raid dual action insect repellent and home freshener and raid shield (currently not commercially available), sc johnson, racine wi) were evaluated for spatial repellent effects against female aedes aegypti (l.) mosquitoes under laboratory (wind tunnel) and semi-field (outdoor enclosure) conditions. the placement of either product in the wind tunnel significantly reduced host-seeking behaviors. the mean baseline (control) landing counts for the ...201728511270
reemergence of yellow fever in ethiopia after 50 years, 2013: epidemiological and entomological investigations.yellow fever (yf) is a viral hemorrhagic disease transmitted by aedes mosquito species. approximately, 200,000 cases and 30,000 deaths occur worldwide every year. in ethiopia, the last outbreak was reported in 1966 with 2200 cases and 450 deaths. a number of cases with deaths from unknown febrile illness reported from south ari district starting from november 2012. this investigation was conducted to identify the causative agent, source of the outbreak and recommend appropriate interventions.201728506254
aedes aegypti (l.) mosquitoes in trinidad, west indies: longevity case studies.few laboratory and field studies have reported long survival periods for ae. aegypti females and even fewer have designed experiments to characterize this important life history trait. this study was conducted under laboratory conditions to determine the number of blood meals taken by individual females, the number of eggs laid per individual female, the length of the gonotrophic cycle, and the duration of female survival. the results showed individual females oviposited between 670 and 1,500 eg ...201728504438
influence of shading and pedestrian traffic on the preference of aedes (stegomyia) aegypti (diptera: culicidae) for oviposition microenvironments.aedes aegypti mosquitoes are highly adaptable to abiotic stimuli. to evaluate the influence of shading and pedestrian traffic on the preference of ae. aegypti for oviposition microenvironments, 20 sites were sampled weekly using ovitraps within the perimeter of universidade federal do espírito santo, located in são mateus, espírito santo, brazil. a spatial and statistical analysis was performed in order to assess the relationship between shading time, pedestrian traffic, and the presence of biol ...201728504433
isolation of an insulin-like peptide from the asian malaria mosquito, anopheles stephensi, that acts as a steroidogenic gonadotropin across diverse mosquito taxa.many insulin-like peptides (ilps) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. antiserum produced to ilp3 in aedes aegypti was used in a radioimmunoassay to monitor the purification of an ilp from heads of adult an. stephensi and recognized the ilp in other immunoassays. the structure of the purified peptide matched that predicted for the ilp3 in this species. the native form stimulated ecdysteroid production by ovaries ...201728502740
metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer.ribosome inactivating proteins (rips) are rna n-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28s rrna. these enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. recently, we reported for the first time in silico evidence of rip encoding genes in metazoans, in two closely related species of insects: aedes aegypti and culex quinquefasciatus. here, we have experimentally confirmed the p ...201728500327
larval interference competition between the native neotropical mosquito limatus durhamii and the invasive aedes aegypti improves the fitness of both species.interspecific competition with native species during biological invasions can sometimes limit alien expansion. we aimed to determine the potential ecological effects of limatus durhamii theobald 1901, a native neotropical mosquito (diptera: culicidae) species, on the invasive species aedes (stegomyia) aegypti (linnaeus 1762) that breeds in the same artificial water containers. development time and adult dry mass were measured in 3 rearing conditions: control (a single larva), intraspecific compe ...201728497885
assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in aedes aegypti (l.).resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. the present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of aedes aegypti selected for 15 generations. the enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-s-transferases were determined during selection, along with biological parameters.201728485040
what does heat tell a mosquito? characterization of the orientation behaviour of aedes aegypti towards heat sources.the use of heat as a cue for the orientation of haematophagous insects towards hot-blooded hosts has been acknowledged for many decades. in mosquitoes, thermoreception has been studied at the molecular, physiological and behavioural levels, and the response to heat has been evaluated in multimodal contexts. however, a direct characterization of how these insects evaluate thermal sources is still lacking. in this study we characterize aedes aegypti thermal orientation using a simple dual choice p ...201728479122
eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of aedes aegypti.in this study, larvicidal activity of silver nanoparticles (agnps) synthesised using apple extract against fourth instar larvae of aedes aegypti was determined. as a result, the agnps showed moderate larvicidal effects against ae. aegypti larvae (lc50 = 15.76 ppm and lc90 = 27.7 ppm). in addition, comparison of larvicidal activity performance of agnps at high concentration prepared using two different methods showed that ae. aegypti larvae was fully eliminated within the duration of 2.5 h. from ...201728476997
photo-induced and phytomediated synthesis of silver nanoparticles using derris trifoliata leaf extract and its larvicidal activity against aedes aegypti.due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. the present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (agnps) produced by derris trifoliata leaf extract in relation to the various c ...201728460330
allergens involved in the cross-reactivity of aedes aegypti with other arthropods.cross-reactivity between aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described.201728434865
n-aryl-n'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes.vector control of disease-transmitting mosquitoes by insecticides has a central role in reducing the number of parasitic- and viral infection cases. the currently used insecticides are efficient, but safety concerns and the development of insecticide-resistant mosquito strains warrant the search for alternative compound classes for vector control. here, we have designed and synthesized thiourea-based compounds as non-covalent inhibitors of acetylcholinesterase 1 (ache1) from the mosquitoes anoph ...201728433681
identification of the septate junction protein gliotactin in the mosquito aedes aegypti: evidence for a role in increased paracellular permeability in larvae.septate junctions (sjs) regulate paracellular permeability across invertebrate epithelia. however, little is known about the function of sj proteins in aquatic invertebrates. in this study, a role for the transmembrane sj protein gliotactin (gli) in the osmoregulatory strategies of larval mosquito (aedes aegypti) was examined. differences in gli transcript abundance were observed between the midgut, malpighian tubules, hindgut and anal papillae of a. aegypti, which are epithelia that participate ...201728432154
allatotropin: a pleiotropic neuropeptide that elicits mosquito immune responses.allatotropins (at) are neuropeptides with pleotropic functions on a variety of insect tissues. they affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. the present work provides experimental evidence that at elicits immune responses in two important mosquito disease vectors, anopheles albimanus and aedes aegypti. hemocytes and an immune-competent mosquito cell line responded to at by showing strong mor ...201728426765
efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae).the current zika health crisis in the americas has created an intense interest in mosquito control methods and products. mosquito vectors of zika are of the genus aedes, mainly the yellow fever mosquito, aedes aegypti. l. the use of repellents to alter mosquito host seeking behavior is an effective method for the prevention of mosquito-borne diseases. a large number of different spray-on repellents and wearable repellent devices are commercially available. the efficacies of many repellents are u ...201728423421
habitat and density of oviposition opportunity influences aedes aegypti (diptera: culicidae) flight distance.understanding the dispersal of aedes (aegypti (l.) diptera: culicidae) after consuming a potentially infectious bloodmeal is an important part of controlling the spread of the arboviruses it transmits. because of the impact on abundance, removal of oviposition sites is a key component of vector control. however, source reduction around a case may encourage dispersal of potentially infected vectors. we compare the effect of oviposition site availability on ae. aegypti dispersal behavior within 30 ...201728419273
the impact of temperature and body size on fundamental flight tone variation in the mosquito vector aedes aegypti (diptera: culicidae): implications for acoustic lures.aedes aegypti (l.) males use female flight tone as a means of mate localization. by playing the sound of a flying female, males can be attracted to a trap to monitor mosquito populations and the progress of transgenic male releases. however, the female flight tone used to attract males needs to be optimized to maximize trap effectiveness. the fundamental frequency of female flight tone could be influenced by both body size and ambient temperature. however, no analysis yet has considered both the ...201728402550
immunohistochemical mapping and transcript expression of the gpa2/gpb5 receptor in tissues of the adult mosquito, aedes aegypti.gpa2/gpb5 is a glycoprotein hormone found in most bilateral metazoans including the mosquito, aedes aegypti. to elucidate physiological roles and functions of gpa2/gpb5, we aim to identify prospective target tissues by examining the tissue- and sex-specific expression profile of its receptor, the leucine-rich repeat-containing g protein-coupled receptor 1 (lgr1) in the adult mosquito. western analyses using a heterologous system with cho-k1 cells, transiently expressing a. aegypti lgr1, yielded ...201728401307
in silico validation of d7 salivary protein-derived b- and t-cell epitopes of aedes aegypti as potential vaccine to prevent transmission of flaviviruses and togaviruses to humans.mosquito (aedes aegyptii) salivary proteins play a crucial role in facilitating viral transmission from vector-to-host due to their role in facilitating the "blood meal" of the vector. three main proteins, d7, aegyptin and sialokinin play a role in this process. using in-silico programs, we identified b- and t-cell epitopes in the mosquito salivary proteins d7 long and short form. t-cell epitopes with high affinity to the most prevalent hla mhc class-i supertypes among different population group ...201729225429
meta-analysis of aedes aegypti expression datasets: comparing virus infection and blood-fed transcriptomes to identify markers of virus presence.the mosquito aedes aegypti (l.) is vector of several arboviruses including dengue, yellow fever, chikungunya, and more recently zika. previous transcriptomic studies have been performed to elucidate altered pathways in response to viral infection. however, the intrinsic coupling between alimentation and infection were unappreciated in these studies. feeding is required for the initial mosquito contact with the virus and these events are highly dependent. addressing this relationship, we reinterr ...201729376049
a small-scale investigation into the effect of a larvicidal oil on oviposition site preference by aedes aegypti.larvicidal oils can be used to control immature aedes aegypti and other pestiferous mosquitoes. to test whether oil-based larvicides also act as oviposition deterrents, indoor and semi-field trials were conducted at the navy entomology center of excellence in jacksonville, fl. in both studies, treatment cages consisted of oviposition cups lined with seed germination paper as an oviposition substrate and filled with 1-wk-old southern live oak ( quercus virginiana) leaf litter-infused water. treat ...201729369032
comparison of adult mosquito abundance from biogents-2 sentinel and biogents gravid aedes traps in northeastern florida.we compared the efficacy of the biogents gravid aedes trap (bg-gat) and biogents-2 sentinel (bg2s) trap to assess differences in adult mosquito collection rates between these 2 mosquito surveillance methods in northeastern florida. species diversity of trap collections was significantly greater for bg2s than for bg-gat, with 14 mosquito species collected from the former trap and 4 species from the latter. aedes aegypti and culex quinquefasciatus were the 2 predominant species collected in both t ...201729369031
bg-sentinel™ trap efficacy as a component of proof-of-concept for push-pull control strategy for dengue vector mosquitoes.the efficacy of the bg-sentinel™ (bgs) trap as a "pull" component of a "push-pull" system (pps) for management of the dengue vector, aedes aegypti, was evaluated using local households in pu tuey, kanchanaburi, thailand. the pull component was the concluding phase of a 3-part investigation using a pps combination spatial repellent (sr) and bgs trap to capture adult vector mosquitoes. two sentinel households were selected for evaluation of bgs trap efficacy based on the highest pretrial indoor re ...201729369029
laboratory evaluation of synthetic blends of l-(+)-lactic acid, ammonia, and ketones as potential attractants for aedes aegypti.attraction of aedes aegypti to various binary, trinary, and quaternary blends of lactic acid and ketones with or without ammonia was studied using a dual choice olfactometer. a dose dependent attraction was observed in cases of single compounds where cyclopentanone attracted the highest percentage (36.9 ± 1.8%) of ae. aegypti when tested alone. no significant difference was observed between the attraction levels of trinary and binary blends of lactic acid and acetone or butanone when tested agai ...201729369028
species specific rna a-to-i editing of mosquito rdl modulates gaba potency and influences agonistic, potentiating and antagonistic actions of ivermectin.the insect gaba receptor, rdl, is the target of several classes of pesticides. the peptide sequences of rdl are generally highly conserved between diverse insects. however, rna a-to-i editing can effectively alter amino acid residues of rdl in a species specific manner, which can affect the potency of gaba and possibly insecticides. we report here that rna a-to-i editing alters the gene products of rdl in three mosquito disease vectors, recoding five amino acid residues in rdl of aedes aegypti a ...201729223796
field evaluation of indoor thermal fog and ultra-low volume applications for control of aedes aegypti in thailand.efficacies of a handheld thermal fogger (patriot™) and a backpack ultra-low volume (ulv) sprayer (twister™) with combinations of 2 different adulticides (pyrethrin, deltamethrin) and an insect growth regulator (pyriproxyfen) were field-tested and compared for their impact on reducing indoor aedes aegypti populations in thailand. the effectiveness of the indoor space sprays was evaluated by sampling the natural ae. aegypti population in houses and determining their physiological status, by monito ...201728590217
citrus seed oils efficacy against larvae of aedes aegypti.dengue fever is a serious public health issue in pakistan for many years. globally plants have been reported to contain compounds with insecticidal properties. these properties have been demonstrated more recently on the larval stages of mosquitoes. therefore, citrus cultivar seeds were evaluated for larvicidal potential against the primary dengue vector aedes aegypti.201729322059
relative insecticidal efficacy of three spatial repellent integrated light sources against aedes aegypti.three repellent products, off! mosquito lamp, insecticandel, and rescue decoshield, were comparatively evaluated against aedes aegypti in 130-m2 enclosed areas with a 317-m3 air volume. the results showed that the off! mosquito lamp with metofluthrin had a greater effect than the insecticandel with transfluthrin, which had greater effect than the decoshield with lemongrass oil and several other plant oils against ae. aegypti. the off! mosquito lamp was the only product to exceed 50% mortality. a ...201729369020
larvicidal potential of juglans regia male flower against anopheles stephensi, aedes aegypti and culex quinquefasciatus.the diseases vectored by mosquitoes continue to be a main cause of illnesses and death throughout the world. the methanol extract of juglans regia male flower was screened for larvicidal activity against three therapeutically important mosquito vectors viz., malarial vector, anopheles stephensi; dengue vector, aedes aegypti and the filarial vector, culex quinquefasciatus. the larvicidal activity was assayed against the early fourth-instar larvae of tested mosquito species at a concentration rang ...201729251995
the attractiveness of the gravid aedes trap to dengue vectors in fiji.the gravid aedes trap (gat) is a passive trap that relies on visual and olfactory cues to lure and capture gravid mosquitoes. the gat was designed as a dengue vector surveillance tool for use in conditions where power sources are not readily available. experiments were conducted over a 10-d period in fiji to determine which species of mosquitoes the gat would collect, how different infusion types affect the attractiveness of the trap, how long each infusion type took to begin attracting mosquito ...201729244174
persistence of toxic activity of fermentation extracts from bacillus thuringiensis var. israelensis after more than three decades of storage.this study was carried out to determine the persistence of toxicity of fermentation extracts of bacillus thuringiensis var. israelensis after more than three decades of storage. for this purpose, a population of aedes aegypti was established. the mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified who protocol. the extracts with the highest mortality rate were determined in triplicat ...201729358951
[estimation of underreporting of chikungunya virus infection cases in girardot, colombia, from november, 2014, to may, 2015].chikungunya virus infection in colombia became epidemic in 2015. it is estimated that there is underreporting of cases to the public health surveillance system which can induce bias in epidemiological projections for decision making, a serious problem, as it veils the real magnitude and actual epidemiological importance of this disease.201729373771
larvicidal activity against aedes aegypti and chemical characterization of the inflorescences of tagetes patula.the crude acetone extract (cae) of defatted inflorescences of tagetes patula was partitioned into five semipurified fractions: n-hexane (hf), dichloromethane (df), ethyl acetate (eaf), n-butanol (bf), and aqueous (aqf). bf was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to hsccc, where patuletin and patulitrin were isolated. cae and the fractions bf, eaf, df, and aqf were analyzed by lc-dad-ms, and patuletin and patulitrin were ...201729362590
synergistic effect of fertilizer and plant material combinations on the development of aedes aegypti (diptera: culicidae) and anopheles gambiae (diptera: culicidae) mosquitoes.chemical fertilizers are used everywhere, which often pollute the breeding sites of mosquitoes. in this laboratory study, the consequences on aedes aegypti (l.) (diptera: culicidae) and anopheles gambiae (giles) (diptera: culicidae) of water-containing plant matter (pm) alone, or in association with an npk type of fertilizer (pm+npk), were evaluated. to obtain a 20% imaginal emergence of an. gambiae (iet20), the bioassays carried out with pm have evidenced that its larvae need four times as much ...201729309617
dengue and yellow fever virus vectors: seasonal abundance, diversity and resting preferences in three kenyan cities.the transmission patterns of dengue (denv) and yellow fever (yfv) viruses, especially in urban settings, are influenced by aedes (stegomyia) mosquito abundance and behavior. despite recurrent dengue outbreaks on the kenyan coast, these parameters remain poorly defined in this and other areas of contrasting dengue endemicity in kenya. in assessing the transmission risk of denv/yfv in three kenyan cities, we determined adult abundance and resting habits of potential aedes (stegomyia) vectors in ki ...201729284522
genetically modified aedes aegypti to control dengue: a review.dengue is an acute infectious disease of viral etiology characterized by lymphadenopathy, leucopenia, headache, biphasic fever, pain in various parts of the body, rashes, and extreme physical weakness. it is a vector-borne disease caused by a positive-stranded rna virus of the family flaviviridae, genus flavivirus. dengue inflicts a significant health, economic, and social burden on populations of endemic areas. dengue virus is transmitted to humans by the mosquito vector aedes aegypti. vaccines ...201729283327
family level variation in wolbachia-mediated dengue virus blocking in aedes aegypti.the mosquito vector aedes aegypti is responsible for transmitting a range of arboviruses including dengue (denv) and zika (zikv). the global reach of these viruses is increasing due to an expansion of the mosquito's geographic range and increasing urbanization and human travel. vector control remains the primary means for limiting these diseases. wolbachia pipientis is an endosymbiotic bacterium of insects that has the ability to block the replication of pathogens, including flaviviruses such as ...201729282144
african and asian zika virus isolates display phenotypic differences both in vitro and in vivo.zika virus (zikv) is a mosquito-borne member of the genus flavivirus that has emerged since 2007 to cause outbreaks in africa, asia, oceania, and most recently, in the americas. here, we used an isolate history as well as genetic and phylogenetic analyses to characterize three low-passage isolates representing african (ard 41525) and asian (cpc-0740, sv0127-14) lineages to investigate the potential phenotypic differences in vitro and in vivo. the african isolate displayed a large plaque phenotyp ...201729280428
altitudinal distribution of aedes indices during dry season in the dengue endemic area of central java, indonesiaaedes mosquitoes, mainly aedes aegypti and aedes albopictus, are the primary and secondary vectors of dengue viruses in indonesia, with transmission occurring by sucking blood. the density of the vectors is influenced by season and rainfall, but limited by altitude. the aim of the study is to describe the density and distribution of dengue vectors during the dry season based on the altitudes of recent dengue cases in five regencies of central java province, indonesia. mosquito larvae and pupae w ...201729274215
zika virus: an emerging infectious disease with serious perinatal and neurologic complications.zika virus (zikv) is a flavivirus that is primarily transmitted by aedes aegypti, the mosquito vector also important in the transmission of the flaviviruses responsible for dengue fever, yellow fever and chikungunya. due to occurrence in the same geographic regions, serological cross-reactivity, and similar, albeit often less severe clinical manifestations as dengue and chikungunya infections, zikv infection likely went undetected and/or misdiagnosed for many years. zikv is somewhat unique among ...201729273403
history and emergence of zika virus.zika virus was discovered in east africa in 1947 by the rockefeller foundation during investigations on the ecology of yellow fever. although it was subsequently shown to have widespread distribution in africa and asia, it was not known to cause epidemics until 2007. this paper describes the history of the virus discovery, emergence and evolution as an epidemic virus, and the its evolving clinical spectrum.201729267917
zika virus mosquito vectors: competence, biology, and vector control.zika virus (zikv) (flaviviridae, flavivirus) has become one of the most medically important mosquito-borne viruses because of its ability to cause microcephaly in utero and guillain-barré syndrome in adults. this virus emerged from its sylvatic cycle in africa to cause an outbreak in yap, federated states of micronesia in 2007, french polynesia in 2014, and most recently south america in 2015. the rapid expansion of zikv in the americas largely has been due to the biology and behavior of its vec ...201729267910
chikungunya: molecular aspects, clinical outcomes and pathogenesis.the alarming worldwide emergence of the chikungunya virus began in the last decade. since the first autochthonous transmission in mexico in november of 2014, the virus has spread throughout the country, resulted in multiple outbreaks. this virus produces an acute and self-limiting disease characterized by fever, polyarthralgia, myalgia, exanthema, and general malaise. it is transmitted to humans by the bite of aedes aegypti and a. albopictus mosquitoes. the fact that the clinical presentation is ...201729265115
characteristics of aedes aegypti adult mosquitoes in rural and urban areas of western and coastal kenya.aedes aegypti is the main vector for yellow fever, dengue, chikungunya and zika viruses. recent outbreaks of dengue and chikungunya have been reported in kenya. presence and abundance of this vector is associated with the risk for the occurrence and transmission of these diseases. this study aimed to characterize the presence and abundance of ae. aegypti adult mosquitoes from rural and urban sites in western and coastal regions of kenya. presence and abundance of ae. aegypti adult mosquitoes wer ...201729261766
individual co-variation between viral rna load and gene expression reveals novel host factors during early dengue virus infection of the aedes aegypti midgut.dengue virus (denv) causes more human infections than any other mosquito-borne virus. the current lack of antiviral strategies has prompted genome-wide screens for host genes that are required for denv infectivity. earlier transcriptomic studies that identified denv host factors in the primary vector aedes aegypti used inbred laboratory colonies and/or pools of mosquitoes that erase individual variation. here, we performed transcriptome sequencing on individual midguts in a field-derived ae. aeg ...201729261661
assessment of maldi-tof mass spectrometry for filariae detection in aedes aegypti mosquitoes.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is an emerging tool for routine identification of bacteria, archaea and fungi. it has also been recently applied as an accurate approach for arthropod identification. preliminary studies have shown that the maldi-tof ms was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and plasmodium parasites, respectively. the aim of the present study was to test the efficien ...201729261659
origin of a high-latitude population of aedes aegypti in washington, dc.an overwintering population of aedes aegypti has been documented in the capitol hill neighborhood of washington, dc, since 2011. mitochondrial cytochrome oxidase i (mtcoi) sequence data presented in a previous study traced the origin to the new world. here, we use microsatellite and snp chip data along with more mitochondrial dna (mtdna) sequences on washington ae. aegypti samples and samples from potential sources to further narrow the origin of this population. genetically, washington ae. aegy ...201729260658
from ground pools to treeholes: convergent evolution of habitat and phenotype in aedes mosquitoes.invasive mosquito species are responsible for millions of vector-borne disease cases annually. the global invasive success of aedes mosquitoes such as aedes aegypti and aedes albopictus has relied on the human transport of immature stages in container habitats. however, despite the importance of these mosquitoes and this ecological specialization to their widespread dispersal, evolution of habitat specialization in this group has remained largely unstudied. we use comparative methods to evaluate ...201729258425
zika virus exhibits lineage-specific phenotypes in cell culture, in aedes aegypti mosquitoes, and in an embryo model.zika virus (zikv) has quietly circulated in africa and southeast asia for the past 65 years. however, the recent zikv epidemic in the americas propelled this mosquito-borne virus to the forefront of flavivirus research. based on historical evidence, zikv infections in africa were sporadic and caused mild symptoms such as fever, skin rash, and general malaise. in contrast, recent asian-lineage zikv infections in the pacific islands and the americas are linked to birth defects and neurological dis ...201729258204
seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan.to find out the variations in larval and adult density of aedes aegypti in different seasons under different ecological conditions.201729256519
social-ecological factors and preventive actions decrease the risk of dengue infection at the household-level: results from a prospective dengue surveillance study in machala, ecuador.in ecuador, dengue virus (denv) infections transmitted by the aedes aegypti mosquito are among the greatest public health concerns in urban coastal communities. community- and household-level vector control is the principal means of controlling disease outbreaks. this study aimed to assess the impact of knowledge, attitudes, and practices (kaps) and social-ecological factors on the presence or absence of denv infections in the household.201729253873
an approach of the exact linearization techniques to analysis of population dynamics of the mosquito aedes aegypti.dengue fever, chikungunya and zika are caused by different viruses and mainly transmitted by aedes aegypti mosquitoes. these diseases have received special attention of public health officials due to the large number of infected people in tropical and subtropical countries and the possible sequels that those diseases can cause. in severe cases, the infection can have devastating effects, affecting the central nervous system, muscles, brain and respiratory system, often resulting in death. vaccin ...201729253493
aedes aegypti resistance development to commonly used insecticides in jakarta, indonesia.aedes aegypti is the primary vector of various relevant arthropod-borne viral infectious diseases worldwide. the mosquito control is still mainly performed by using insecticides but their effectiveness is increasingly questioned nowadays. we here conducted a study on ae. aegypti resistance development towards several commonly used insecticides in the capital city of jakarta, indonesia. in order to achieve this goal, ae. aegypti eggs from jakarta were collected with ovitraps and hatched in the in ...201729253003
analytical report of the 2016 dengue outbreak in córdoba city, argentina.after elimination of the aedes aegypti vector in south america in the 1960s, dengue outbreaks started to reoccur during the 1990s; strongly in argentina since 1998. in 2016, córdoba city had the largest dengue outbreak in its history. in this article we report this outbreak including spatio-temporal analysis of cases and vectors in the city. a total of 653 dengue cases were recorded by the laboratory-based dengue surveillance system and georeferenced by their residential addresses. case maps wer ...201729239555
assessing natural infection with zika virus in the southern house mosquito, culex quinquefasciatus, during 2016 in puerto rico.the epidemic of zika in the western hemisphere has led to intense investigations of all species important in the transmission of zika virus (zikv), including putative mosquito vectors. although evidence points to stegomyia (= aedes) (diptera: culicidae) mosquitoes as the primary vectors in nature among humans, there remains the possibility that other common mosquito species may be implicated in the rapid spread of the virus. herein, field-caught culex quinquefasciatus (diptera: culicidae) collec ...201729239003
aedes aegypti breeding site in an underground rainwater reservoir: a warning.we describe the discovery of aedes aegypti underground breeding site in the pinheiros neighborhood of são paulo, sp, during an entomological survey program performed in 2016. even with intense surveillance and vector control, large numbers of mosquitoes were present in this area. a detailed investigation allowed for the detection of ae. aegypti in an underground reservoir used for rainwater storage. after the implementation of protection screens in the accesses, the presence of the vector was no ...201729236877
detection of zika virus in aedes mosquitoes from mexico.we report on the results of an entomovirological surveillance system of aedes populations performed by the ministry of health of the central state of san luis potosí, mexico.201729232453
efficacy of 13 commercial household aerosol insecticides against aedes aegypti (diptera: culicidae) from morelos, mexico.in mexico, aedes aegypti (l.) (diptera: culicidae) is the primary vector of dengue, zika, and chikungunya viruses. control programs include community participation using personal protection such as household aerosol insecticides. in both, urban or rural areas, the use of aerosol insecticides is a common practice to avoiding mosquito biting. thus, information on the efficacy of commercial products must be available. this study reports the efficacy of 13 household aerosol insecticides against ae. ...201729228197
the first detected airline introductions of yellow fever mosquitoes (aedes aegypti) to europe, at schiphol international airport, the netherlands.air-borne introduction of exotic mosquitoes to schiphol airport in the netherlands has been considered plausible based upon findings of mosquitoes in aircraft cabins during 2008, 2010 and 2011. beginning in 2013, surveillance efforts at schiphol had focused on promptly detecting accidental introductions at the airport facilities in order to quickly react and avoid temporary proliferation or establishment of mosquito populations, identify the origin of the introductions, and avoid potential trans ...201729221490
full-length genome sequence of a chikungunya virus isolate from the 2017 autochthonous outbreak, lazio region, italy.we report here the genome sequence of a human chikungunya virus isolate from the ongoing autochthonous outbreak in central italy. the sequence (east-central-south african lineage, indian ocean sublineage), which is similar to recent sequences from pakistan and india, shows e1 and e2 signatures of strains whose main mosquito vector is aedes aegypti, although aedes albopictus is the vector in italy.201729217791
the gastric caecum of larval aedes aegypti: stimulation of epithelial ion transport by 5-hydroxytryptamine and camp.we report measurements of ion transport across the gastric caecum of larvae of aedes aegypti, a vector of yellow fever that inhabits a variety of aquatic habitats ranging from freshwater to brackish water. we provide the first measurements of the effect of 5-hydroxytryptamine (5-ht) on transepithelial potential (tep), luminal ion concentrations and electrochemical potentials, as well as basolateral membrane potential and h+, na+ and k+ fluxes. tep, basolateral membrane potential, and h+, k+, and ...201729217627
the immune strategies of mosquito aedes aegypti against microbial infection.yellow fever mosquito aedes aegypti transmits many devastating arthropod-borne viruses (arboviruses), such as dengue virus, yellow fever virus, chikungunya virus, and zika virus, which cause great concern to human health. mosquito control is an effective method to block the spread of infectious diseases. ae. aegypti uses its innate immune system to fight against arboviruses, parasites, and fungi. in this review, we briefly summarize the recent findings in the immune response of ae. aegypti again ...201729217264
differences in prevalence of symptomatic zika virus infection by age and sex-puerto rico, 2016.during the zika virus (zikv) outbreak in puerto rico in 2016, non-pregnant women aged 20-39 years were disproportionately identified with zikv disease. we used household-based cluster investigations to determine if this disparity was associated with age- or sex-dependent differences in the rate of zikv infection or reporting symptoms.201729216376
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