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in vitro activity of fosfomycin against gram-negative urinary pathogens and the biological cost of fosfomycin resistance.the aim of this study was to reassess the activity of fosfomycin against recently isolated uropathogens circulating in italy and to evaluate the effect of fosfomycin resistance on the expression of several virulence traits using the rare mutant strains. in vitro activity of fosfomycin was evaluated using 441 gram-negative organisms isolated from patients with uncomplicated urinary tract infections (utis). fosfomycin was the most active antibiotic against escherichia coli (99% susceptibility). th ...200314527772
urinary tract infections in south croatia: aetiology and antimicrobial resistance.the aim of this study was to determine the distribution of uropathogens isolated from outpatients living in south croatia and the in vitro susceptibility of these organisms to antimicrobial agents. of 5080 enrolled uropathogen isolates, 82.28% isolates were gram-negative, the most frequent isolates being escherichia coli (62.62%), enterococci (10.18%), proteus mirabilis (5.31%), streptococcus agalactiae (3.84%), staphylococcus spp. (3.70%), pseudomonas spp. (3.46%), klebsiella spp. (2.38%). the ...200314527773
antimicrobial sensitivity pattern of organisms causing urinary tract infection in children with sickle cell anaemia in ibadan, nigeria.as part of a larger project on childhood urinary tract infection, antimicrobial sensitivity tests were carried out on the bacterial isolates from the urine of febrile children seen at the university college hospital, ibadan, nigeria.200314529216
antimicrobial activity of some 2- and 3-pyridinyl-1h-benzimidazoles and their feiii, cuii, znii, and agi complexes.2-(2-pyridinyl)- (li), 2-(6-methyl-2-pyridinyl)- (lii), 2-(6-methyl-2-pyridinyl)-5-methyl-(liii), 2-(3-pyridinyl)- (liv), 2-(3-pyridinyl)-5-methyl-1h-benzimidazoles (lv) and their complexes with fe(no3)3, cu(no3)2, zn(no3)2, and agno3 were synthesized and antibacterial activity of the compounds was tested toward staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella typhi, shigella flexneri, proteus mirabilis and antifungal ...200314533478
effect of nacl concentration on the survival and growth of coliforms in raw seasoned sausages. 200314535413
are infections due to resistant pathogens associated with a worse outcome in critically ill patients?to evaluate the outcome of critically ill patients infected with antimicrobial resistant microorganisms, and to analyse the factors involved in the development of antimicrobial resistance.200314556755
structure of the o-polysaccharide leads to classification of proteus penneri 31 in proteus serogroup o19.o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide (lps) of proteus penneri strain 31. sugar and methylation analyses along with nmr spectroscopic studies, including 2d 1h,1h cosy, tocsy, roesy, 1h,13c and 1h,31p hmqc experiments, demonstrated the following structure of the polysaccharide: [carbohydrate structure: see text] where fucnac is 2-acetamido-2,6-dideoxygalactose and etnp is 2-aminoethyl phosphate. the polysaccharide studied has the same carbohydrate backbo ...200314556999
structure of the o-polysaccharide of proteus penneri 28 and proteus vulgaris o31 and classification of p. penneri 26 and 28 in proteus serogroup o31.the lipopolysaccharides (lps) of proteus penneri 28 and proteus vulgaris o31 (prk 55/57) were degraded with dilute acetic acid and structurally identical high-molecular-mass o-polysaccharides were isolated by gel-permeation chromatography. sugar analysis and nuclear magnetic resonance (nmr) spectroscopic studies showed that both polysaccharides contain d-glcnac, 2-acetamido-2,6-dideoxy-l-glucose (l-2-acetamido-2,6-dideoxyglucose (n-acetylquinovosamine)) and 2-acetamido-3-o-[(s)-1-carboxyethyl]-2 ...200314557001
temporomandibular joint involvement in malignant external otitis.the purpose of this study was to present 6 patients with malignant external otitis (meo) that resulted in temporomandibular joint (tmj) involvement and to discuss the incidence, clinical presentation, and treatment modalities.200314561963
structure of the n-acetyl-l-rhamnosamine-containing o-polysaccharide of proteus vulgaris tg 155 from a new proteus serogroup, o55.the o-polysaccharide of the lipopolysaccharide (lps) of proteus vulgaris tg 155 was found to contain 2-acetamido-2,6-dideoxy-l-mannose (n-acetyl-l-rhamnosamine, l-rhanac), a monosaccharide that occurs rarely in nature. the following structure of the o-polysaccharide was established by nmr spectroscopy, including 2d cosy, tocsy, roesy and 1h,13c hsqc experiments, along with chemical methods: [carbohydrate structure in text] rabbit polyclonal o-antiserum against p. vulgaris tg 155 reacted with bot ...200314499576
fusarium infections in burn patients: a case report and review of the literature.fusarium species, or saprophytic molds, are important plant pathogens and recognized as agents of human mycotic infections. frequently superficial, deep-tissue involvement, and dissemination occurs in immunocompromised hosts with hematologic malignancies, aplastic anemia, and chemotherapy treatment. presented in this work is a burn patient with a fatal disseminated infection in addition to a review of the literature. a 40-year-old white male acquired a 73% grease scald injury at work. his hospit ...200314501396
role of aspartate-133 and histidine-458 in the mechanism of tryptophan indole-lyase from proteus vulgaris.tryptophan indole-lyase (trpase) from proteus vulgaris is a pyridoxal 5'-phosphate dependent enzyme that catalyzes the reversible hydrolytic cleavage of l-trp to yield indole and ammonium pyruvate. asp-133 and his-458 are strictly conserved in all sequences of trpase, and they are located in the proposed substrate-binding region of trpase. these residues were mutated to alanine to probe their role in substrate binding and catalysis. d133a mutant trpase has no measurable activity with l-trp as su ...200314503866
beta-lactamase stability of faropenem.faropenem (far) is an orally available member of the penem class unique among carbapenems and other available beta-lactams. this study compared far to cephalosporins and imipenem with respect to beta-lactamase (bla) stability and emergence of resistance to staphylococcus aureus and escherichia coli. bla stability was studied using enzyme preparations from sonicated/centrifuged 24-hour cultures of e. coli, enterobacter cloacae, proteus vulgaris, providencia rettgeri, klebsiella pneumoniae, s. aur ...200314504433
proteus mirabilis and rheumatoid arthritis: no association with the disease.proteus mirabilis(pm) is implicated in different studies in the pathogenesis of rheumatoid arthritis (ra) because of the structural homogeneity of its haemolysin b precursor with eqrraa sequences in drb 1 haplotype. the aim of the study was to compare the levels of antibodies specific to pm in the sera of patients with ra and healthy controls in our population. serum samples from 78 consecutive ra patients and 75 healthy controls were analysed for the presence of igg isotype and total immunoglob ...200314505214
structure of the o-polysaccharide of a serologically separate strain of proteus mirabilis, tg 332, from a new proposed proteus serogroup o50.the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of proteus mirabilis tg 332 strain. the following structure of the o-polysaccharide was determined by chemical methods along with nmr spectroscopy, including 2d cosy, tocsy, roesy and 1h, 13c hmqc experiments: [see equation in text]. the o-polysaccharide studied has a unique structure among proteus o-antigens. accordingly, p. mirabilis tg 332 is serologically separate, and we propose to classify this strain into ...200314505878
in vitro susceptibilities of gram-negative bacteria isolated from hospitalized patients in four european countries, canada, and the united states in 2000-2001 to expanded-spectrum cephalosporins and comparator antimicrobials: implications for therapy.access to current antimicrobial agent surveillance data is an important prerequisite for the optimal management of patients with hospital-acquired infections. the present study used data collected in 2000 to 2001 from 670 laboratories in europe (france, germany, italy, and spain), canada, and the united states to report on the in vitro activities of ceftriaxone, cefotaxime, and comparative agents against >125,000 isolates of gram-negative bacteria from hospitalized patients. all but two isolates ...200314506014
staghorn calculus in renal allograft presenting as acute renal failure.urolithiasis is a rare complication in renal transplant recipients. we report a case of a staghorn calculus occurring in renal allograft, presenting as anuric renal failure with gram-negative sepsis.200314508619
structural studies on the lipopolysaccharide core of proteus ox strains used in weil-felix test: a mass spectrometric approach.the core region of the lipopolysaccharides of proteus group ox bacteria, which are used as antigens in weil-felix test for serodiagnosis of rickettsiosis, were studied by chemical degradations in combination with esi ftms, including infrared multi-photon dissociation (irmpd) ms/ms and capillary skimmer dissociation. structural variants of the inner core region were found to be the same as in proteus non-ox strains that have been studied earlier. the outer core region has essentially the same str ...200314670728
[relationship between antibacterial activity of aloe and its anthaquinone compounds].to investigate the relationship between the antibacterial activity of aloe and its contents of anthaquinone compounds, measure and compale antibacterial activities of aloin and aloe-emodin, and analyse the effect of glycoside on the antibacterial activity of aloin.200315615409
temporary catheter infections in hemodialysis patients: results from a single center in turkey.in this study, we aimed to determine the frequencies of catheter exit-site infection (cesi), catheter-related bloodstream infection (cr-bsi) and catheter colonization (cc); causative microorganisms; and resistance patterns in patients with temporary hemodialysis catheters. from march 1999 to march 2000, 67 hemodialysis patients (38 males, 29 females; median age: 52, range: 17-84) were evaluated. the cdc criteria were used to diagnose cesi, cr-bsi and cc. the tips of catheters were cultured by ma ...200315068127
proteus syndrome and somatic mosaicism of the chromosome 16.the authors describe clinical pathologic findings in a patient with a structural chromosome 16 anomaly in mosaic distribution with the phenotypic characteristics of the proteus syndrome. this is the 1st report showing that the proteus syndrome may be associated with a chromosome 16 anomaly; while previous findings suggested that this syndrome was associated with a modification of chromosome 1. as the pathologic hypothesis of this syndrome possibly involves lethal genes in somatic mosaicism, resp ...200315206168
[incidence and sensitivity to antibiotics of germs isolated from surgical wound infections].we studied 119 bacterial strains isolated from postoperative infected wounds. all these strains were considered to be strains that derived from the same number of nosocomial infections, all the postoperative infections of the wounds being nosocomial infections. regarding their frequency, we isolated the following strains: e. coli--68 strains (57%), s. aureus--37 strains (31%), pseudomonas spp.--9 strains (8%) and proteus spp.--5 strains (4%). we performed the bacteriological study of these strai ...200315341326
antibacterial effects of carica papaya fruit on common wound organisms.the purpose of the study was to investigate antibacterial activity of ripe and unripe carica papaya on selected micro-organisms. cultures of micro-organisms were routinely maintained in nutrient agar slants at 4 degrees c. extracts of immature, mature and ripe carica papaya fruit were obtained by separately grinding factions of the epicarp, endocarp and seeds and filtering them through gauze. sensitivity tests were conducted by adding 0.06 ml of extract to agar wells (6 mm diameter) prepared fro ...200315040064
bacterial dynamics associated with algal antibacterial substances during post harvest desiccation process of sargassum stolonifolium phang et yoshida.brown algae of genus sargassum are known to produce relatively higher amount of alginic acid. optimal extraction of this algalcolloid for local consumption requires in-depth studies on post-harvest treatment of the algal fronds. present investigation endeavors to establish the dynamics and inter-relationship of moisture content and bacteria found on the surface of the alga and alginic acid content during post-harvest desiccation of sargassum stolonifolium phang et yoshida. harvested fronds were ...200315248481
polymicrobial osteomyelitis of the odontoid process with epidural abscess: case report and review of literature.osteomyelitis of the odontoid process is a rare disease and staphylococcus aureus is the usual causative organism. we present a case caused by a synergistic infection with staphylococcus aureus and proteus mirabilis, which has not been reported previously. a high index of suspicion is required for diagnosis, and early institution of broad antimicrobial therapy, including agents active against gram-negative organisms, is mandatory.200315912167
a rapid method for determining mycobacterium tuberculosis based on a bulk acoustic wave impedance biosensor.the bulk acoustic wave impedance biosensor was set up and used to monitor the growth of mycobacterium tuberculosis (m.tb). this sensor is rapid, simple, sensitive (lower limit is 2 x 10(3) cells ml(-1)) and cheap (easy to generalize). the typical response curve was different from other bacteria's, such as escherichia coli, staphylococcus aureus, proteus mirabilis. the frequency detection time was used to quantitatively determine m.tb. it was proportional to logarithm of the initial concentration ...200318968982
evaluation of the antimicrobial activity of the acetone extract of the lichen ramalina farinacea and its (+)-usnic acid, norstictic acid, and protocetraric acid constituents.the acetone extract of the lichen ramalina farinacea and its (+)-usnic acid constituent showed antimicrobial activity against bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocolitica, candida albicans, and candida glabrata. norstictic acid was active against aeromonas hydrophila as well as the above microorganisms except yersinia enterocolitica. protocetraric acid showed activity only against the tested yeasts candida alb ...200418998406
roxithromycin antagonism with essential and trace elements.in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various macrolide antibiotics, the synergistic or antagonistic behavior of roxithromycin metal complexes have been studied and compared with the parent drug. metal complexes of roxithromycin with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium have been investigated for their antibacterial activity and compared with roxithromycin by observing ...200416414588
urinary tract infections associated with multidrug resistant enteric bacilli: characterization and genetical studies.urinary tract infections (utis) are among the most commonly prevalent infections in clinical practice. escherichia coli is the causative agent in about 85% of community-acquired utis, followed by klebsiella that accounts for 6 to 17% of such infections. present study is based on the isolation-identification and antibiotic resistance pattern of about 60 indigenous bacterial isolates from uti patients. prevalence rates were consistent with those from major recent studies reported in the literature ...200416414603
bacteriology of diabetic foot lesions.clinical grading and bacteriological study of 107 patients with diabetic foot lesions revealed polymicrobial aetiology in 69 (64.4%) and single aetiology in 21 (19.6%). among 107 patients 62 had ulcer. of these 31 had mixed aerobes. twenty six patients with cellulitis and 12 with gangrene had more than 5 types of aerobes and anaerobes such as e. coli, klebsiella spp., pseudomonas spp., proteus spp., enterobactor spp., enterococci spp., clostridium perfringens, bacteroides spp., prevotella spp. a ...200417642727
microwave irradiation for shortening the processing time of samples of flagellated bacteria for scanning electron microscopy.microwave irradiation (mwi) has been applied to the development of rapid methods to process biological samples for scanning electron microscopy (sem). in this paper we propose two simple and quick techniques for processing bacteria (proteus mirabilis and vibrio mimicus) for sem using mwi. in the simplest methodology, the bacteria were placed on a cover-glass, air-dried, and submitted to conductivity stain. the reagent used for the conductivity stain was the mordant of a light microscopy staining ...200417061527
bacteriological quality of some pharmaceutical products marketed by drug vendors in uyo, nigeria.bacteriological quality of some pharmaceutical products purchased from open markets, buses and drug stores in uyo metropolis was studied in order to determine the level of contamination of the drugs. the drug samples examined were tetracycline capsules, paracetamol tablets, ampicillin capsules, chloroquine tablets, chloroquine syrup, chloroquine injections, flagyl tablets and chloramphenicol tablets. the bacterial count ranged from 2.3x10(4) cfu/g to 4.4x10(4) cfu/g for the 16 samples of ampicil ...200417298130
depression - the proteus of medicine.depression is discussed as a disease of antiquity with suitable contemporary references also. the prevalence of this disorder, which at a given time constitutes 121 millions world-wide is mentioned. among the types of depression, comorbid depression forms an important one. classical depression forms the visible part of the depression iceberg while somatic and other life contextual situation forms the submerged part. somatic manifestations per se do not carry diagnostic weightage unless the core ...200421408045
oropharyngeal flora changes in patients with head and neck malignancy post radiotherapy.radiotherapy has been recognized as a valuable modality of treatment in the management of head and neck cancers. it can have a direct bactericidal effect on the normal flora of the oropharynx. the objective of this study is to determine the changes in the oropharyngeal flora after external beam radiation. this prospective non randomized control study was performed to aid in identification of organisms involved in sepsis, as well as aid in choosing appropriate antibiotics for surgical procedures ...200415889559
the susceptibility of certain bacterial strains to imidazotriazine derivatives.the synthesized imidazo[2,1-c]triazines were tested for their antimicrobial activity. microbiological screening tests conducted in relation to 54 strains of gram-positive and 52 strains of gram-negative bacteria, 6 strains of yeast-like fungi and 3 strains of moulds showed significant activity of compound 19 against 52 gram-negative bacterial strains. this compound was active against 16 strains of escherichia coli, 12 strains of pseudomonas, 10 strains of proteus spp., 8 strains of klebsiella pn ...200416146057
recurrent urinary tract infections--uropathogen incidence and drug sensitivity.the aim of the study was the retrospective analysis of the results of the drug sensitivity studies of bacterial species cultured from 681 urinary specimens collected from 79 children (12 boys and 67 girls) aged 0-18 years with recurrent urinary tract infection in a period from 1997 to 2001. factors predisposing to recurrent urinary tract infections and the incidences of particular uropathogens were also analyzed. escherichia coli (57.3%), proteus spp. (15.6%) and pseudomonas aeruginosa (9.1%) we ...200416146092
synthesis, spectra and biomimetic properties of copper(ii)-copper(ii) and copper(ii)-zinc(ii) binuclear complexes with cun5 chromophores.x-band electron spin resonance (esr) and uv-vis spectra of a homobinuclear [(bipy)2cu-e-im-cu(bipy)2](bf4)3 and a heterobinuclear [(bipy)2cu-e-im-zn(bipy)2](bf4)3 complexes, e-im=2-ethylimidazolate ion have been described as possible models for superoxide dismutase (sod). magnetic moment and esr spectral measurements of the homobinuclear complex have shown an antiferromagnetic exchange interaction. from ph-dependent esr and uv-vis spectral measurements studies, these complexes have been found to ...200416043054
urinary tract infection in children: incidence of vesicoureteral reflux, imaging studies, and antimicrobial resistance among e. coli. 200415868844
childhood urinary tract infection: a clinical analysis of 597 cases.objectives of study: to gain new insights into the epidemiology, genitourinary (gu) tract anomalies, etiologies, susceptibility of urinary pathogens to antibiotics in children with urinary tract infection (uti) during the past decade in taiwan.200415868848
leucocyte migration and nitroblue tetrazolium assay in nigerian children with bacteremia and malaria parasitemia.the prevalence of malaria parasitemia, bacteremia, certain hematological parameters, leucocyte migration index and nitroblue tetrazolium dye reduction were determined in 147 nigerian children (4.24+/-2.88 years of age). sixty (40.8%), 28(19.1%) and 26(17.7%) had malaria parasitemia only, bacteremia only and both malaria parasitemia and bacteremia, respectively. four genera of bacteria, i.e e. coli, proteus, staphylococcus and salmonella, were detected in subjects with both malaria parasitemia an ...200415783134
[prevalence of proteus mirabilis strains in clinical specimens and evaluation of their resistance to selected antibiotics].proteus mirabilis are opportunistic microorganisms which cause serious hospital infections. the resistance of 1038 strains of p. mirabilis to selected antibiotics (lactams, aminoglycosides, fluoroquinolones, nitrofurantoin, cotrimoxazole, tetracycline) was tested by disk diffusion method. the least of p. mirabilis strains were resistant to imipenem and aztreonam. most of p. mirabilis strains were isolated from urine, wounds and ulcers from patients of departments of urology, dermatology, general ...200415754653
functional characterization of contractile vacuole isolated from amoeba proteus.contractile vacuoles (cvs) released from cells of amoeba proteus were used to analyze its function in vitro. when cv was transferred to a hypertonic medium, its volume decreased within 10 sec. when it was subsequently returned to its original medium, it quickly started swelling. however, it ruptured before recovering its initial volume. these results suggested that the cv membrane is semi-permeable and that the fluid is collected by the osmotic gradient in vivo. the water permeability of membran ...200415665503
[urinary tract infections in children--etiologic agents and susceptibility to antibiotics].urinary tract infections (utis) are common clinical problem in pediatric hospitals. the aim of this study was to compare the incidence of utis during two different time periods (from february 1999 to march 2000, and from april 2000 to september 2001) in pediatric patients treated in ambulatory or hospitalized in new children hospital. the frequency of occurrence of esbl--producing gram-negative rods increased during second period study. the isolated esbl--producing strains were resistant also to ...200415765758
[urolithiasis in hemodialysis patients]. 200415250327
gene cloning, expression and characterization of novel phytase from obesumbacterium proteus.the gene phya encoding phytase was isolated from obesumbacterium proteus genomic library and sequenced. the cleavage site of the phya signal peptide was predicted and experimentally proved. the phya protein shows maximum identity of 53% and 47% to phosphoanhydride phosphorylase from yersinia pestis and phytase appa from escherichia coli, respectively. based on protein sequence similarity of phya and its homologs, the phytases form a novel subclass of the histidine acid phosphatase family. to cha ...200415251209
lemierre's syndrome associated with septic pulmonary embolism: a case report.an 18-year-old female patient suffering from a painful right-sided neck mass was admitted to our emergency service. computed tomography of the neck revealed thrombosis of the right internal jugular vein. computed tomography of the chest indicated septic pulmonary emboli in both lungs. blood and oropharyngeal cultures showed proteus that was sensitive to cefepime and amicasin. chemotherapy was administered for 12 days, however, her complaints continued with fulminant progression. an urgent decomp ...200415253264
in vitro activity of ave1330a, an innovative broad-spectrum non-beta-lactam beta-lactamase inhibitor.production of beta-lactamases is the main mechanism of beta-lactam resistance in gram-negative bacteria. despite the current use of clavulanic acid, sulbactam and tazobactam, the prevalence of class a and class c enzymes is increasing worldwide, demanding new beta-lactamase inhibitors. here we report the antimicrobial properties of ave1330a, a representative of a novel class of bridged bicyclico[3.2.1]diazabicyclo-octanones in combination with ceftazidime. materials and methods: ic(50) and kinet ...200415254025
kirby-bauer disc approximation to detect inducible third-generation cephalosporin resistance in enterobacteriaceae.resistance to beta-lactam antibiotics in enteric gram-negative bacilli may be difficult to detect using standard methods of either kirby-bauer disc diffusion (kbdd) or broth dilution for minimal inhibitory concentration (mic). this difficulty is due to genetic differences in resistance determinants, differences in levels of gene expression, and variation in spectra of enzymatic activity against the substrate beta-lactams used for susceptibility testing. we have examined 95 clinical isolates repo ...200415256000
radiologic manifestations of proteus syndrome.proteus syndrome is a sporadic disorder named for its highly variable manifestations. the disease causes tissue overgrowth in a mosaic pattern and may affect tissues derived from any germinal layer. the disease process is not usually apparent at birth but develops rapidly in childhood. common manifestations include macrodactyly, vertebral abnormalities, asymmetric limb overgrowth and length discrepancy, hyperostosis, abnormal and asymmetric fat distribution, asymmetric muscle development, connec ...200415256628
diffuse xanthogranulomatous pyelonephritis and staghorn calculus: report of one case.xanthogranulomatous pyelonephritis and staghorn calculus are rare in children. in this report, we describe a chinese boy without history of urinary tract infection who developed insidious onset of left flank pain. urine culture showed proteus mirabilis infection. sonography and computed tomography of the abdomen showed typical picture of xanthogranulomatous pyelonephritis and staghorn calculus. photomicrography showed characteristic lipid-laden macrophage aggregates. after nephrectomy, he was sy ...200415264707
lesson of the week: perils of pessaries.vaginal pessaries are widely considered to be a safe alternative to surgery in older women. we report a case of near fatal septicaemia in a 75-year-old woman associated with a shelf pessary, the presence of which was identified during an exploratory laparotomy. this case highlights the importance of the gynaecological history and examination when assessing older women with septicaemia of unknown source.200415271640
the three-dimensional structure of catalase from enterococcus faecalis.enterococcus faecalis haem catalase was crystallized using lithium sulfate at neutral ph. the crystals belong to space group r3, with unit-cell parameters a = b = 236.9, c = 198.1 a. the three-dimensional structure was determined by molecular replacement using a subunit of the proteus mirabilis catalase structure. it was refined against 2.3 a synchrotron data to a free r factor of 21.8%. like other catalases, the e. faecalis catalase is a homotetramer with a fold and structure similar to those o ...200415272159
incidence of enteric bacteria and staphylococcus aureus in day care centers in akwa ibom state, nigeria.the incidence of enteric bacteria and staphylococcus aureus in four day care centers in akwa ibom state was studied using culture techniques. the percentage frequencies of the isolates from 124 samples were staphylococcus aureus (33.9), escherichia coli (19.0), klebsiella sp (14.4), citrobacter sp (12.5) and proteus mirabilis (7.4). the sources of contamination were floors, chairs, skin, bed linen, door handles, fans, children's tables, walls, windows, ceiling, headmistress's table and chairs, d ...200415272770
application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections.a rapid and accurate method for detection for common pathogenic bacteria in foodborne infections was established by using oligonucleotide array technology. nylon membrane was used as the array support. a mutation region of the 23s rrna gene was selected as the discrimination target from 14 species (genera) of bacteria causing foodborne infections and two unrelated bacterial species. a pair of universal primers was designed for pcr amplification of the 23s rrna gene. twenty-one species (genera)-s ...200415279944
structure of the o-polysaccharide of proteus serogroup o34 containing 2-acetamido-2-deoxy-alpha-d-galactosyl phosphate.on mild acid degradation of the lipopolysaccharide of proteus vulgaris o34, strain ccug 4669, the o-polysaccharide was cleaved at a glycosyl-phosphate linkage that is present in the main chain. the resultant phosphorylated oligosaccharides and an alkali-treated lipopolysaccharide were studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, and the following structure of the branched tetrasaccharide phosphate repeating unit of the o-polysaccharide was established: [carbo ...200415280059
frequency and diversity of class a extended-spectrum beta-lactamases in hospitals of the auvergne, france: a 2 year prospective study.to evaluate the frequency and diversity of extended-spectrum beta-lactamases (esbls) produced by enterobacteriaceae and pseudomonas aeruginosa in one french region.200415282240
epidermal nevus syndromes: clinical findings in 35 patients.of the patients with epidermal nevi, 10-18% may have disorders of the eye, nervous, and musculoskeletal systems. a predisposition to malignant neoplasms in ectodermal and mesodermal structures may also be found. there are six different epidermal nevus syndromes described so far: proteus, congenital hemidysplasia with ichthyosiform nevus and limb defect syndrome, phakomatosis pigmentokeratotica, sebaceous nevus, becker nevus, and nevus comedonicus. thirty-five patients with epidermal nevus syndro ...200415283784
anesthesia for proteus syndrome.proteus syndrome is a complex disorder comprising malformations and overgrowth of multiple tissues. the disorder is highly variable affecting tissues in a mosaic pattern. a 2-year-old boy with proteus syndrome, with epidermal verrucal naevus, hyperplastic lesions of connective tissue, hyperostosis, overgrowth of tubular bones, bilateral inguinal hernia, and juvenile intestinal polyposis was scheduled for vertebral magnetic resonance imaging (mri) for further evaluation of malignancies. in additi ...200415283831
emerging resistance among bacterial pathogens in the intensive care unit--a european and north american surveillance study (2000-2002).globally icus are encountering emergence and spread of antibiotic-resistant pathogens and for some pathogens there are few therapeutic options available.200415283864
epidermal nevus syndromes.the term "epidermal nevus syndrome" (ens) has been used to describe the association of epidermal hamartomas and extra-cutaneous abnormalities. epidermal nevi follow the lines of blaschko. the majority of the extra-cutaneous manifestations involve the brain, eye, and skeletal systems. several subsets with characteristic features have been delineated including the nevus sebaceous syndrome, proteus syndrome, child syndrome, becker nevus syndrome, nevus comedonicus syndrome, and phakomatosis pigment ...200415295924
investigating the antimicrobial activity of natural honey and its effects on the pathogenic bacterial infections of surgical wounds and conjunctiva.antimicrobial activities of 10-100% (wt/vol) concentrations of new honey, stored honey, heated honey, ultraviolet-exposed honey, and heated stored honey were tested against common human pathogens, including escherichia coli, entrobacter cloacae, pseudomonas aeruginosa, shigella dysenteriae, klebsiella sp., haemophilus influenzae, proteus sp., staphylococcus aureus, streptococcus hemolyticus group b, and candida albicans. antimicrobial activity of honey was tested in acidic, neutral, or alkaline ...200415298770
ascending to a uti vaccine? 200415040255
the possible influence of micro-organisms and putrefaction in the production of ghb in post-mortem biological fluid.in recent years, the post-mortem production of the drug of abuse gamma-hydroxybutyric acid (ghb) in biological fluids (e.g. blood and urine) has caused various interpretative problems for toxicologists. previously, other researchers have shown certain microbial species (pseudomonas spp. and clostridium aminobutyricum) possess the necessary enzymes to convert gaba to ghb. a preliminary investigation involving putrefied post-mortem blood indicated there was no observed relationship between "endoge ...200415040914
tyrosine phenol-lyase and tryptophan indole-lyase encapsulated in wet nanoporous silica gels: selective stabilization of tertiary conformations.the pyridoxal 5'-phosphate-dependent enzymes tyrosine phenol-lyase and tryptophan indole-lyase were encapsulated in wet nanoporous silica gels, a powerful method to selectively stabilize tertiary and quaternary protein conformations and to develop bioreactors and biosensors. a comparison of the enzyme reactivity in silica gels and in solution was carried out by determining equilibrium and kinetic parameters, exploiting the distinct spectral properties of catalytic intermediates and reaction prod ...200415044726
antibacterial and antipeptide antibodies in japanese and finnish patients with rheumatoid arthritis.it has been suggested that proteus infection may be involved in the pathogenesis of rheumatoid arthritis (ra). bacterial and peptide immune responses in patients with ra and other control subjects were investigated in two geographically different populations. serum samples from finnish patients with early ( n=72) and advanced ( n=27) ra and 30 finnish healthy controls, as well as from japanese ra patients from two different locations: tokyo ( n=30) and otsu ( n=30), 18 patients with systemic lup ...200415045628
assay method for antitumor l-methionine gamma-lyase: comprehensive kinetic analysis of the complex reaction with l-methionine.l-methionine gamma-lyase (ec 4.4.1.11) is a pyridoxal 5'-phosphate-dependent multifunctional enzyme. measuring the initial velocity of alpha-ketobutyrate production by alpha,gamma-elimination of l-methionine catalyzed by l-methionine gamma-lyase is not very feasible, because the enzyme simultaneously catalyzes both gamma-replacement and alpha,gamma-elimination. to develop an accurate enzyme assay, the comprehensive enzyme kinetics needed to be elucidated by progress curve analysis on the basis o ...200415051540
serological characterization of the o-specific polysaccharide of providencia alcalifaciens o23.the genus providencia belongs to the enterobacteriaceae family and currently consists of five species: p. alcalifaciens, p. heimbachae, p. rettgerii, p. rustigianii and p. stuartii. the serological classification scheme of p. alcalifaciens, p. rustigianii and p. stuartii includes 63 o-serogroups and 30 h-serogroups. the o-antigenic specificity is defined by the structure of the o-antigen (o-specific polysaccharide--ops), a part of the lipopolysaccharide (lps, endotoxin), one of the major compone ...200415053232
comparative study of the in vitro activity of a new fluoroquinolone, abt-492.the in vitro activity of a new fluoroquinolone, abt-492, was determined.200415056651
possible occurrence of a crabtree effect in the production of lactic and butyric acids by a floc-forming bacterial consortium.lactic and butyric acid production by bacterial flocs in a continuous culture obeyed different physiological constraints. the butyric acid rate of production was constant and independent of the growth rate [0.012 +/- 0.001 gbut/(l.h)], whereas lactic fermentation occurred only beyond a critical growth rate (0.25 +/- 0.05 h(-1)) and was apparently associated with an abrupt drop in biomass. principles of modeling used to describe a crabtree effect in saccharomyces cerevisiae were found to apply to ...200415057470
island nail transfer in the treatment of macrodactyly of the great toe: a case report.macrodactyly involving the great toe is difficult to treat when both the functional and cosmetic problems are addressed simultaneously. partial resection of the distal phalanx is an easy way to reduce the length of the involved toe. however, there are cosmetic concerns with loss of the toenail. the authors report on a clinical case in which shortening and debulking of the toe was performed while preserving the hallux nail. the nail complex was transferred as an island flap to the dorsal aspect o ...200415057859
effects of pnpg on cell growth cycle, motility machinery and quorum sensing in serratia marcescens.p-nitrophenylglycerol (pnpg) effectively inhibits swarming of the enterobacterium proteus mirabilis. the underlying mechanism of inhibition is unclear. we have now found that both pnpg also inhibits motility and swarming in another enterobacterium, serratia marcescens. while the peak promoter activities of the flagellar master operon (flhdcsm), the flagellin structural gene (hagsm) and the nuclease gene (nucasm) in s. marcescens increased with increasing pnpg concentration, the expression of the ...200415060680
maxillary sinus disease of odontogenic origin.odontogenic sinusitis is a well-recognized condition and accounts for approximately 10% to 12% of cases of maxillary sinusitis. an odontogenic source should be considered in patients with symptoms of maxillary sinusitis who give a history positive for odontogenic infection or dentoalveolar surgery or who are resistant to standard sinusitis therapy. diagnosis usually requires a thorough dental and clinical evaluation with appropriate radiographs. common causes of odontogenic sinusitis include den ...200415064067
[a case report of douglas abscess due to diverticulitis of the sigmoid colon associated with liver abscess]. 200415065348
myocardial necrosis in a captive binturong (arctictis binturong).an adult male binturong, arctictis binturong, which had been anorexic and lethargic for seven days became acutely dyspnoeic and died under anaesthesia. a postmortem examination revealed left ventricular hypertrophy with a thrombus occluding the left ventricular chamber. histological findings included moderate to severe multifocal, vasculocentric myocardial degeneration and necrosis with fibrosis replacing myocardiocytes. escherichia coli and proteus mirabilis were grown on cultures. the animal's ...200415160846
images in clinical medicine. unusual cystitis. 200415163778
[acid phosphatase of ameba: amoeba proteus]. 200415174335
[bact-alert automatized system for blood cultures: 5 vs 7 days of incubation. first argentine multicentre study].bact-alert automatized system for blood cultures: 5 vs 7 days of incubation. first argentine multicentre study. between january and december 2001, we analyzed 80,141 blood cultures by the bact-alert system (14,960 fan aerobics, 3,855 fan anaerobic, 11,114 standards aerobics, 11,367 standards anaerobic, 12,054 pediatrics and 26,791 fan pediatrics bottles) and 44.235 series from 27.615 patients at eight hospitals of buenos aires city, one of la plata city and three of the buenos aires province. a ...200415174746
synthesis, absolute stereochemistry and molecular design of the new antifungal and antibacterial antibiotic produced by streptomyces sp.201.the absolute stereochemistry of the new antifungal and antibacterial antibiotic produced by streptomyces sp.201 has been established by achieving the total synthesis of the product. a series of analogues have also been synthesized by changing the side chain and their bioactivity assessed against different microbial strains. among them, 1e (r = c8h17) was found to be the most potent with mic of 8 microg/ml against mycobacterium tuberculosis, 12 microg/ml against escherichia coli and 16 microg/ml ...200415177476
characterization and serological classification of a collection of proteus penneri clinical strains.bacteria of the genus proteus, which are a common cause of urinary tract infections, are divided into four species: p. mirabilis, p. vulgaris, p. penneri, and p. hauseri, and three unnamed genomospecies, proteus 4, 5, and 6 (single-strain species p. myxofaciens was isolated from the gypsy moth). establishing the serological classification of these species would aid in completing the classification scheme of the whole genus proteus and in applying serological methods in diagnostic procedures and ...200415179326
screening of selected indigenous plants of lebanon for antimicrobial activity.the objective of this study is to test in vitro the antimicrobial efficacy of 39 water and 39 methanol extracts derived from different parts of 27 indigenous wild plant species that have been commonly used in lebanese folk medicine. the antimicrobial efficacy was determined using the single disk diffusion method, with 10 and 20 microl load extract volume per disc. nine test microorganisms were used namely, escherichia coli, proteus sp., pseudomonas aeruginosa, shigella dysenteria, salmonella ent ...200415182897
prosthesis culture 6 months after implantation and the effect of haematogenous seeding in a rat model.the aim of this study was to investigate the role of haematogenous seeding in late prosthesis infection. in all, 45 male wistar albino rats were divided into three equal groups. a small piece of silicone prosthesis was implanted into the scrotum of all rats under sterile condition and antibiotic prophylaxis. in the first group, all silicones were removed after 6 months without any complication and were cultured for bacterial growth. in the second group, a disc, which was saturated with staphyloc ...200415184914
interactions between high pressure homogenization and antimicrobial activity of lysozyme and lactoperoxidase.it was the objective of this work to evaluate the effect of high pressure homogenization on the activity of antimicrobial enzymes such as lysozyme and lactoperoxidase against a selected group of gram positive and gram negative species inoculated in skim milk. lactobacillus helveticus, lactobacillus plantarum and listeria monocytogenes were the most pressure resistant species while bacillus subtilis, pseudomonas putida, salmonella typhimurium, staphylococcus aureus, proteus vulgaris and salmonell ...200415193800
evaluation of the tensile strengths of four monofilament absorbable suture materials after immersion in canine urine with or without bacteria.to evaluate the tensile strength, elongation, and degradation of 4 monofilament absorbable suture materials that undergo degradation by hydrolysis in specimens of canine urine with various physical characteristics.200415198227
artificial neural networks for prediction of antibacterial activity in series of imidazole derivatives.artificial neural networks (anns) have been applied for the quantitative structure-activity relationships (qsar) studies of antibacterial activity against escherichia coli, serratia marcescens, proteus vulgaris, klebsiella pneumoniae and pseudomonas aeruginosa of a large series of new imidazole derivatives. antibacterial activity against individual bacteria, expressed as logarithm of reciprocal of the minimal inhibitory concentrations, log 1/mic, has been related to a number of physicochemical a ...200415200380
metalloantibiotics: synthesis and antibacterial activity of cobalt(ii), copper(ii), nickel(ii) and zinc(ii) complexes of kefzol.kefzol (kzl), a beta-lactam antibiotic, possesses various donor sites for interaction with transition metal(ii) ions [co(ii), cu(ii), ni(ii) and zn(ii)] to form complexes of the type [m(kzl)2]cl2 and [m(kzl)cl], with molar ratio of metal: ligand (m:l) of 1:2 and 1:1 respectively. these complexes were prepared and characterized by physicochemical and spectroscopic methods. their ir and nmr spectra suggest that kefzol potentially acts as a bidentate, tridentate as well as monoanionic tetradentate ...200415202497
[total infarction of the penis caused by entrapment in a plastic bottle].penile strangulation injuries are rare emergency situations, but need a fast urological emergency management for decompression of the penis and for removing of the foreign bodies. strangulation objects can be metallic or non-metallic objects and are usually used for erotic or autoerotic purposes, for masturbation or increasing erection. we report a case of a 62-year old male with a total infected gangrene of the penis caused by a strangulation of a plastic bottle neck which continued 14 days. a ...200415205739
[molecular mechanisms of cephalosporin resistance in gram-negative bacteria of the enterobacteriaceae family].extended spectrum beta lactamase genes were detected by the pcr in 87.6% of 231 enterobacteriaceae strains isolated in medical institutions of moscow, st. petersburg, tomsk and nazran that showed a decrease in their susceptibility to 3rd generation cephalosporins. alone or in various combinations tem type beta lactamases were detected in 43.3% of the isolates, 46.8 and 51.2% of the isolates produced shv type and ctx type beta lactamases respectively. combinations of 2 and 3 different determinant ...200415344391
[use of therapeutic polycomponent vaccine "immunovac vp-4" for the treatment of chronic bacterial prostatitis].a complex of clinical and laboratory examinations of patients with bacterial chronic prostatitis was carried out. the patients were divided into two groups: 55 patients treated by the method of immunotherapy and 20 patients (controls) undergoing basic therapy. immunotherapy was carried out with the use of polycomponent vaccine vp-4 containing staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris and escherichia coli antigens. vaccinal therapy was indicated in cases with infection focus ...200415346948
transcription of proteus mirabilis flaab.proteus mirabilis, a gram-negative urinary tract pathogen, has two highly homologous, tandemly arranged flagellin-encoding genes, flaa and flab. flaa is transcribed from a sigma(28) promoter, while flab is a silent allele. previous studies have demonstrated the presence of a family of hybrid flagellin genes, referred to as flaab. these genes are composed of the 5' end of flaa and the 3' end of flab, and are produced through excision of the intervening dna between the two genes. although the exis ...200415347745
digestive pathology of sea turtles stranded in the canary islands between 1993 and 2001.digestive lesions were observed in 84 of 136 sea turtles (128 caretta caretta, four chelonia mydas and four dermochelys coriacea) stranded in the canary islands between january 1993 and december 2001. in the oral cavity ulcerative and necropurulent stomatitis were the most frequently observed lesions, and in the oesophagus ulcerative and fibrinous oesophagitis, and traumatic oesophageal perforation were most frequently observed; all these lesions were mainly associated with the ingestion of fish ...200415357377
poly(vinyl chloride)-coated sol-gels for studying the effects of nitric oxide release on bacterial adhesion.nitric oxide (no) releasing sol-gel materials coated with poly(vinyl chloride) (pvc) films exhibit increased stability at ambient and physiological temperatures. the polymer overcoat, however, reduces the no fluxes by 5-35% over the initial week of release. the variation in no fluxes between unmodified and pvc-coated sol-gels is negligible after 7 days. the pvc polymeric layer provides controlled surface chemistry for systematic studies of the effects of no release on bacterial adhesion. as an e ...200415360321
surgical infections: a microbiological study.surgical infections are mostly polymicrobial, involving both aerobes and anaerobes. one hundred seventeen cases comprised of abscesses (n=51), secondary peritonitis (n=25), necrotizing fascitis (n=22) and wounds with devitalized tissues (n=19) were studied. the number of microorganisms isolated per lesion was highest in secondary peritonitis (2.32). the aerobe/ anaerobe ratio was 0.81 in secondary peritonitis and 1.8 in necrotizing fascitis. most secondary peritonitis (80%), necrotizing fascitis ...200415361989
bacterial resistance to antimicrobials in urinary isolates.escherichia coli accounted for about 80% of organisms in uncomplicated urinary tract infections (utis), followed by staphylococcus spp. especially staphylococcus saprophyticus, and proteus mirabilis. against e. coli isolates from patients with uncomplicated uti, faropenem was the most effective. up to 1999, fluoroquinolone-resistant isolates were not observed in patients with uncomplicated uti, but in 2001 fluoroquinolone-resistant e. coli isolates emerged and accounted for about 8%. various typ ...200415364302
urinary tract infections in african infants.urinary tract infection (uti) causes significant illness in the first 2 years of life. the diagnosis in most developing countries is often overlooked due to the tedious nature of obtaining urine from these young ones who would not void voluntarily. misdiagnosis very often lead to avoidable ill health and long-term renal damage. there is a need to diagnose uti in febrile infants to alert clinicians. a prospective study of febrile infants aged up to 12 months on admission was undertaken in a 6-mon ...200415364303
the role of unusual pathogens in prostatitis syndrome.a total of 1442 patients with symptoms of chronic prostatitis were examined over a 4-year period at the outpatient department for urogenital infections, university hospital for infectious diseases "dr. fran mihaljevic", zagreb, croatia. an infectious aetiology was determined in 1070 (74.2%) patients. in 561 of 1070 (52.4%) patients the inflammatory finding (>10 wbc/hpf) was found in expressed prostatic secretions (eps) or voided bladder urine (vb(3)). normal, <10 wbcs/hpf was found in 362 of 536 ...200415364308
nosocomial transmission of ctx-m-2 beta-lactamase-producing acinetobacter baumannii in a neurosurgery ward.three strains of cefotaxime (ctx)-resistant acinetobacter baumannii, fm0209680, fm0300106, and fm0301433, were isolated from transtracheal aspirate cultures of three patients with probable nosocomial infections in a neurosurgery ward in japan. the ctx mics for these isolates were greater than 128 microg/ml but were drastically reduced in the presence of 4 microg of clavulanic acid per ml. these strains were also resistant to ceftriaxone, cefpodoxime, and aztreonam but were susceptible to ceftazi ...200415364979
microbiological quality of fermented cassava flour 'kpor umilin'.a study was conducted on the microbiological quality of traditionally fermented cassava flour 'kpor umilin' and a laboratory modification of the method developed with a view to upgrading the traditional process. microbial analysis of samples from both processing methods was evaluated using standard microbiological methods. the bacterial count in the traditionally processed flours ranged from 2.7 x 10(3) to 1.2 x 10(7) cfu/g, while the modified sample had a count of 3.5 x 10(2) cfu/g. the predomi ...200415369985
diagnosis of proteus syndrome was correct. 200415372511
mutation analysis of the tumor suppressor pten and the glypican 3 (gpc3) gene in patients diagnosed with proteus syndrome.proteus syndrome is a complex hamartomatous disorder characterized by asymmetrical gigantism, epidermal nevi, vascular malformations, hamartomas, lipomas, and hyperostosis. since the syndrome was first described, many hypotheses have been proposed to explain its occurrence. the most plausible is happle's somatic mosaic hypothesis, but no somatic mutations in candidate genes have been reported to be clearly involved in proteus syndrome. however, germ-line pten mutations have been reported in pati ...200415372512
reassessment of the proteus syndrome literature: application of diagnostic criteria to published cases.the medical care of patients affected by rare disorders depends heavily on experiences garnered from prior cases, including those patients evaluated by the treating physician and those published in the medical literature. the utility of published cases is wholly dependent upon accurate diagnosis of those patients. in our experience, the rate of misdiagnosis in proteus syndrome (ps) is high. diagnostic criteria have been published, but these criteria have not been applied consistently and were pu ...200415372514
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