Publications

fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression.	diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. diapause-destined insects store large amounts of fat when preparing for diapause. however, the extent to which these accumulated fat reserves influence diapause remains unclear. to address this question, we investigated the function of fatty acid synthase (fas), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause prepar ...	2017	28071706
dengue in latin america: systematic review of molecular epidemiological trends.	dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (denv-1, -2, -3 or -4). a literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in latin america, including the caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research ...	2017	28068335
proteomic analysis of cry2aa-binding proteins and their receptor function in spodoptera exigua.	the bacterium bacillus thuringiensis produces crystal (cry) proteins that are toxic to a diverse range of insects. transgenic crops that produce bt cry proteins are grown worldwide because of their improved resistance to insect pests. although bt "pyramid" cotton that produces both cry1a and cry2a is predicted to be more resistant to several lepidopteran pests, including spodoptera exigua, than plants that produce cry1ac alone, the mechanisms responsible for the toxicity of cry2aa in s. exigua a ...	2017	28067269
wolbachia infections in aedes aegypti differ markedly in their response to cyclical heat stress.	aedes aegypti mosquitoes infected with wolbachia bacteria are currently being released for arbovirus suppression around the world. their potential to invade populations and persist will depend on interactions with environmental conditions, particularly as larvae are often exposed to fluctuating and extreme temperatures in the field. we reared ae. aegypti larvae infected with different types of wolbachia (wmel, walbb and wmelpop-cla) under diurnal cyclical temperatures. rearing wmel and wmelpop-c ...	2017	28056065
the dangers of accepting a single diagnosis: case report of concurrent plasmodium knowlesi malaria and dengue infection.	dengue and malaria are two common, mosquito-borne infections, which may lead to mortality if not managed properly. concurrent infections of dengue and malaria are rare due to the different habitats of its vectors and activities of different carrier mosquitoes. the first case reported was in 2005. since then, several concurrent infections have been reported between the dengue virus (denv) and the malaria protozoans, plasmodium falciparum and plasmodium vivax. symptoms of each infection may be mas ...	2017	28049485
pterodon emarginatus oleoresin-based nanoemulsion as a promising tool for culex quinquefasciatus (diptera: culicidae) control.	preparation of nanoformulations using natural products as bioactive substances is considered very promising for innovative larvicidal agents. on this context, oil in water nanoemulsions develop a main role, since they satisfactorily disperse poor-water soluble substances, such as herbal oils, in aqueous media. pterodon emarginatus, popularly known as sucupira, has a promising bioactive oleoresin. however, to our knowledge, no previous studies were carried out to evaluate its potential against cu ...	2017	28049483
insecticidal toxicities of carvacrol and thymol derived from thymus vulgaris lin. against pochazia shantungensis chou &lu., newly recorded pest.	the insecticidal toxicities of five essential oils against pochazia shantungensis adults and nymphs, newly recorded pests, were evaluated. the lc50 values of thymus vulgaris, ruta graveolens, citrus aurantium, leptospermum petersonii and achillea millefolium oils were recorded as 57.48, 84.44, 92.58, 113.26 and 125.78 mg/l, respectively, against p. shantungensis nymphs using the leaf dipping bioassay, and 75.80, 109.86, 113.26, 145.06 and 153.74 mg/l, respectively, against p. shantungensis adult ...	2017	28106093
rna interference mediated knockdown of brugia malayi udp-galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae.	galactofuranose is an essential cell surface component present in bacteria, fungi and several nematodes such as caenorhabditis spp., brugia spp., onchocerca spp. and strongyloides spp. this sugar maintains the integrity of parasite surface and is essential for virulence. udp-galactopyranose mutase (bmugm) plays a key role in galf biosynthesis by catalyzing conversion of udp-galactopyranose into udp-galactofuranose and knockout studies of the gene in leishmania major, mycobacterium and aspergillu ...	2017	28103957
antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections.	the transmission of dengue virus (denv) and chikungunya virus (chikv) has increased worldwide, due in part to the lack of a specific antiviral treatment. for this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. the objective of this study was to identify some of the compounds that are present in mammea americana (m. americana) and tabernaemontana cymosa (t. cymosa) plants and, subsequently, to evaluate their cyto ...	2017	28100218
microrna-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.	micrornas (mirnas) are key regulators of developmental processes, such as cell fate determination and differentiation. previous studies showed dicer knockdown in honeybee embryos disrupt the processing of functional mature mirnas and impairs embryo patterning. here we investigated the expression profiles of mirnas in honeybee embryogenesis and the role of the highly conserved mir-34-5p in the regulation of genes involved in insect segmentation. a total of 221 mirnas were expressed in honey bee e ...	2017	28098233
the physical genome mapping of anopheles albimanus corrected scaffold misassemblies and identified interarm rearrangements in genus anopheles.	the genome of the neotropical malaria vector anopheles albimanus was sequenced as part of the 16 anopheles genomes project published in 2015. the draft assembly of this species consisted of 204 scaffolds with an n50 scaffold size of 18.1 mb and a total assembly size of 170.5 mb. it was among the smallest genomes with the longest scaffolds in the 16 anopheles species cluster, making an. albimanus the logical choice for anchoring the genome assembly to chromosomes. in this study, we developed a hi ...	2017	27821634
a comparative analysis of three vector-borne diseases across australia using seasonal and meteorological models.	ross river virus (rrv), barmah forest virus (bfv), and dengue are three common mosquito-borne diseases in australia that display notable seasonal patterns. although all three diseases have been modeled on localized scales, no previous study has used harmonic models to compare seasonality of mosquito-borne diseases on a continent-wide scale. we fit poisson harmonic regression models to surveillance data on rrv, bfv, and dengue (from 1993, 1995 and 1991, respectively, through 2015) incorporating s ...	2017	28071683
mitigating future avian malaria threats to hawaiian forest birds from climate change.	avian malaria, transmitted by culex quinquefasciatus mosquitoes in the hawaiian islands, has been a primary contributor to population range limitations, declines, and extinctions for many endemic hawaiian honeycreepers. avian malaria is strongly influenced by climate; therefore, predicted future changes are expected to expand transmission into higher elevations and intensify and lengthen existing transmission periods at lower elevations, leading to further population declines and potential extin ...	2017	28060848
the search for novel insecticide targets in the post-genomics era, with a specific focus on g-protein coupled receptors.	insects are considered pests globally, implicated in the destruction of agricultural fields and transmission of pathogens that cause deadly human diseases, such as dengue, zika and malaria. the diversity of the insecticide arsenal has remained stagnant for decades, but the recent rise of insecticide resistance fueled the discovery of novel modes of action, and the power of genomics has reinvigorated this search. this review discusses the importance of comparative and functional insect genomics i ...	2017	28076467
the transcriptome of the mosquito aedes fluviatilis (diptera: culicidae), and transcriptional changes associated with its native wolbachia infection.	wolbachia is a bacterial endosymbiont that naturally infects a wide range of insect species, and causes drastic changes to host biology. stable infections of wolbachia in mosquitoes can inhibit infection with medically important pathogens such as dengue virus and malaria-causing plasmodium parasites. however, some native wolbachia strains can enhance infection with certain pathogens, as is the case for the mosquito aedes fluviatilis, where infection with plasmodium gallinaceum is enhanced by the ...	2017	28049478
functional analysis of the abcs of eye color in helicoverpa armigera with crispr/cas9-induced mutations.	many insect pigments are localized in subcellular pigment granules, and transport of pigment precursors from the cytoplasm is accomplished by abc proteins. drosophila melanogaster has three half-transporter genes (white, scarlet, and brown, all affecting eye pigments) and bombyx mori has a fourth (ok). the white, brown, scarlet and ok proteins each have one transmembrane and one cytoplasmic domain and they heterodimerize to form functional transporters with different substrate specificities. we ...	2017	28053351
species specific rna a-to-i editing of mosquito rdl modulates gaba potency and influences agonistic, potentiating and antagonistic actions of ivermectin.	the insect gaba receptor, rdl, is the target of several classes of pesticides. the peptide sequences of rdl are generally highly conserved between diverse insects. however, rna a-to-i editing can effectively alter amino acid residues of rdl in a species specific manner, which can affect the potency of gaba and possibly insecticides. we report here that rna a-to-i editing alters the gene products of rdl in three mosquito disease vectors, recoding five amino acid residues in rdl of aedes aegypti a ...	2017	29223796
field evaluation of indoor thermal fog and ultra-low volume applications for control of aedes aegypti in thailand.	efficacies of a handheld thermal fogger (patriot™) and a backpack ultra-low volume (ulv) sprayer (twister™) with combinations of 2 different adulticides (pyrethrin, deltamethrin) and an insect growth regulator (pyriproxyfen) were field-tested and compared for their impact on reducing indoor aedes aegypti populations in thailand. the effectiveness of the indoor space sprays was evaluated by sampling the natural ae. aegypti population in houses and determining their physiological status, by monito ...	2017	28590217
citrus seed oils efficacy against larvae of aedes aegypti.	dengue fever is a serious public health issue in pakistan for many years. globally plants have been reported to contain compounds with insecticidal properties. these properties have been demonstrated more recently on the larval stages of mosquitoes. therefore, citrus cultivar seeds were evaluated for larvicidal potential against the primary dengue vector aedes aegypti.	2017	29322059
relative insecticidal efficacy of three spatial repellent integrated light sources against aedes aegypti.	three repellent products, off! mosquito lamp, insecticandel, and rescue decoshield, were comparatively evaluated against aedes aegypti in 130-m2 enclosed areas with a 317-m3 air volume. the results showed that the off! mosquito lamp with metofluthrin had a greater effect than the insecticandel with transfluthrin, which had greater effect than the decoshield with lemongrass oil and several other plant oils against ae. aegypti. the off! mosquito lamp was the only product to exceed 50% mortality. a ...	2017	29369020
larvicidal potential of juglans regia male flower against anopheles stephensi, aedes aegypti and culex quinquefasciatus.	the diseases vectored by mosquitoes continue to be a main cause of illnesses and death throughout the world. the methanol extract of juglans regia male flower was screened for larvicidal activity against three therapeutically important mosquito vectors viz., malarial vector, anopheles stephensi; dengue vector, aedes aegypti and the filarial vector, culex quinquefasciatus. the larvicidal activity was assayed against the early fourth-instar larvae of tested mosquito species at a concentration rang ...	2017	29251995
the attractiveness of the gravid aedes trap to dengue vectors in fiji.	the gravid aedes trap (gat) is a passive trap that relies on visual and olfactory cues to lure and capture gravid mosquitoes. the gat was designed as a dengue vector surveillance tool for use in conditions where power sources are not readily available. experiments were conducted over a 10-d period in fiji to determine which species of mosquitoes the gat would collect, how different infusion types affect the attractiveness of the trap, how long each infusion type took to begin attracting mosquito ...	2017	29244174
in silico validation of d7 salivary protein-derived b- and t-cell epitopes of aedes aegypti as potential vaccine to prevent transmission of flaviviruses and togaviruses to humans.	mosquito (aedes aegyptii) salivary proteins play a crucial role in facilitating viral transmission from vector-to-host due to their role in facilitating the "blood meal" of the vector. three main proteins, d7, aegyptin and sialokinin play a role in this process. using in-silico programs, we identified b- and t-cell epitopes in the mosquito salivary proteins d7 long and short form. t-cell epitopes with high affinity to the most prevalent hla mhc class-i supertypes among different population group ...	2017	29225429
meta-analysis of aedes aegypti expression datasets: comparing virus infection and blood-fed transcriptomes to identify markers of virus presence.	the mosquito aedes aegypti (l.) is vector of several arboviruses including dengue, yellow fever, chikungunya, and more recently zika. previous transcriptomic studies have been performed to elucidate altered pathways in response to viral infection. however, the intrinsic coupling between alimentation and infection were unappreciated in these studies. feeding is required for the initial mosquito contact with the virus and these events are highly dependent. addressing this relationship, we reinterr ...	2017	29376049
[estimation of underreporting of chikungunya virus infection cases in girardot, colombia, from november, 2014, to may, 2015].	chikungunya virus infection in colombia became epidemic in 2015. it is estimated that there is underreporting of cases to the public health surveillance system which can induce bias in epidemiological projections for decision making, a serious problem, as it veils the real magnitude and actual epidemiological importance of this disease.	2017	29373771
dengue vectors in urban and suburban assam, india: entomological observations.	dengue is rapidly becoming established in north-east india and spreading, on account of rapid urbanization and population movement, with reported morbidity and attributable death cases. this study aims to determine the seasonal abundance of aedes (stegomyia) albopictus and aedes (stegomyia) aegypti in guwahati metropolis and suburban settlements; to characterize the breeding resources for these mosquitoes; and to ascertain the status of their susceptibility to adulticides and larvicides.	2017	28607255
copper ii - polar amino acid complexes: toxicity to bacteria and larvae of aedes aegypti.	control strategies using insecticides are sometimes ineffective due to the resistance of the insect vectors.in this scenario new products must be proposed for the control of insect vectors.the complexes l-aspartate cu (ii) and l-glutamate-cu (ii) complexes were synthesized and characterized by elemental analysis, visible ultraviolet, infrared spectroscopy and potentiometric titration. the toxicity of these complexes was analyzed in aedes aegypti (diptera: culicidae) larvae and gram-negative and ...	2017	28746551
jaburetox: update on a urease-derived peptide.	urease from canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. these proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kda), which is ...	2017	28638403
bacteria-mediated hypoxia functions as a signal for mosquito development.	mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. we previously reported that several mosquito species, including aedes aegypti, do not develop beyond the first instar when fed a nutritionally complete diet in the absence of a gut microbiota. in contrast, several species of bacteria, including escherichia coli, rescue development of axenic larvae into adults. the molecular mechanisms underlying bacteria-dependent growth are unknown. here, we des ...	2017	28630299
new insights into hcv replication in original cells from aedes mosquitoes.	the existing literature about hcv association with, and replication in mosquitoes is extremely poor. to fill this gap, we performed cellular investigations aimed at exploring (i) the capacity of hcv e1e2 glycoproteins to bind on aedes mosquito cells and (ii) the ability of hcv serum particles (hcvsp) to replicate in these cell lines.	2017	28830495
larvicidal potency of marine actinobacteria isolated from mangrove environment against aedes aegypti and anopheles stephensi.	the marine soil samples were collected from different locations of parangipettai mangrove ecosystem, vellar estuary, southeast coast of india. totally 30 different marine actinobacteria were isolated by serial dilution plate technique on starch casein agar medium. the isolated actinobacteria were investigated for their larvicidal activity against aedes aegypti and anopheles stephensi mosquitoes. streptomyces fungicidicus, s. griseus, s. albus, s. alboflavus and s. rochei were identified as poten ...	2017	28615847
efficacy of the vegetative cells of lysinibacillus sphaericus for biological control of insecticide-resistant aedes aegypti.	the control of aedes aegypti is usually based on chemical insecticides, but the overuse of these compounds has led to increased resistance. the binary toxin produced by lysinibacillus sphaericus in the final stages of sporulation is used for mosquito control due to its specificity against the culicid larvae; however, it has been proved that ae. aegypti is refractory for this toxin. currently, there is no evidence of the use of l. sphaericus vegetative cells for mosquito biocontrol. therefore, in ...	2017	28490350
mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of buruli ulcer.	addressing the transmission enigma of the neglected disease buruli ulcer (bu) is a world health organization priority. in australia, we have observed an association between mosquitoes harboring the causative agent, mycobacterium ulcerans, and bu. here we tested a contaminated skin model of bu transmission by dipping the tails from healthy mice in cultures of the causative agent, mycobacterium ulcerans. tails were exposed to mosquito (aedes notoscriptus and aedes aegypti) blood feeding or punctur ...	2017	28410412
vector competence of aedes albopictus (skuse) and aedes aegypti (linnaeus) for plasmodium gallinaceum infection and transmission.	avian malaria caused by plasmodium gallinaceum is an important mosquito-borne disease. eradication of this disease remains problematic since its competent vectors are diverse and widely distributed across the globe. several mosquito species were implicated as competent vectors for this parasite. however, studies on vector competence for p. gallinaceum remain limited. in this study, vector competence in the two most predominant mosquito vectors in tropical countries, aedes albopictus and ae. aegy ...	2017	28579025
synergistic efficacy of aedes aegypti antimicrobial peptide cecropin a2 and tetracycline against pseudomonas aeruginosa.	the increasing prevalence of antibiotic resistance has created an urgent need for alternative drugs with new mechanisms of action. antimicrobial peptides (amps) are promising candidates that could address the spread of multidrug-resistant bacteria, either alone or in combination with conventional antibiotics. we studied the antimicrobial efficacy and bactericidal mechanism of cecropin a2, a 36-residue α-helical cationic peptide derived from aedes aegypti cecropin a, focusing on the common pathog ...	2017	28483966
aedes aegypti piwi4 is a noncanonical piwi protein involved in antiviral responses.	the small interfering rna (sirna) pathway is a major antiviral response in mosquitoes; however, another rna interference pathway, the piwi-interacting rna (pirna) pathway, has been suggested to be antiviral in mosquitoes. piwi4 has been reported to be a key mediator of this response in mosquitoes, but it is not involved in the production of virus-specific pirnas. here, we show that piwi4 associates with members of the antiviral exogenous sirna pathway (ago2 and dcr2), as well as with proteins of ...	2017	28497119
characterization of tolypocladium cylindrosporum (hypocreales: ophiocordycipitaceae) and its impact against aedes aegypti and aedes albopictus eggs at low temperature.	we examined the growth characteristics of tolypocladium cylindrosporum ibt 41712 and its potential to infect eggs of aedes aegypti and ae. albopictus at a low temperature (15°c). when grown on sabouraud dextrose agar supplemented with yeast extract, the ibt 41712 formed white colonies turning to a slightly darker, off-white color when mature. the mycelia bore swollen conidiophores producing smooth-walled, oblong to cylindrical conidia with varying sizes, ranging from 1.5 to 3.5 μm long. to deter ...	2017	28854112
the role of the environment in the evolution of tolerance and resistance to a pathogen.	defense against parasites can be divided into resistance, which limits parasite burden, and tolerance, which reduces pathogenesis at a given parasite burden. distinguishing between the two and understanding which defense is favored by evolution in different ecological settings are important, as they lead to fundamentally different evolutionary trajectories of host-parasite interactions. we let the mosquito aedes aegypti evolve under different food levels and with either no parasite, a constant p ...	2017	28829641
specificity and putative mode of action of a mosquito larvicidal toxin from the bacterium xenorhabdus innexi.	reduction of mosquito-borne diseases relies, in part, on the use of synthetic pesticides to control pest mosquitoes. this reliance has led to genetic resistance, environmental contamination and the nondiscriminatory elimination of both pest and non-pest species. to expand our options for control, we screened entomopathogenic bacteria for potential larvicidal activity. a lipopeptide from the bacterium, xenorhabdus innexi, was discovered that displayed potent larvicidal activity. the lc50s of the ...	2017	28712711
potential risk of re-emergence of urban transmission of yellow fever virus in brazil facilitated by competent aedes populations.	yellow fever virus (yfv) causing a deadly viral disease is transmitted by the bite of infected mosquitoes. in brazil, yfv is restricted to a forest cycle maintained between non-human primates and forest-canopy mosquitoes, where humans can be tangentially infected. since late 2016, a growing number of human cases have been reported in southeastern brazil at the gates of the most populated areas of south america, the atlantic coast, with rio de janeiro state hosting nearly 16 million people. we sh ...	2017	28687779
taking innovative vector control interventions in urban latin america to scale: lessons learnt from multi-country implementation research.	prior to the current public health emergency following the emergence of chikungunya and zika virus disease in the americas during 2014 and 2015, multi-country research investigated between 2011 and 2013 the efficacy of novel aedes aegypti intervention packages through cluster randomised controlled trials in four latin-american cities: fortaleza (brazil); girardot (colombia), acapulco (mexico) and salto (uruguay). results from the trials led to a scaling up effort of the interventions at city lev ...	2017	28829235
modeling the transmission and control of zika in brazil.	zika virus, a reemerging mosquito-borne flavivirus, started spread across central and southern america and more recently to north america. the most serious impacted country is brazil. based on the transmission mechanism of the virus and assessment of the limited data on the reported suspected cases, we establish a dynamical model which allows us to estimate the basic reproduction number r 0 = 2.5020. the wild spreading of the virus make it a great challenge to public health to control and preven ...	2017	28798323
differential outcomes of zika virus infection in aedes aegypti orally challenged with infectious blood meals and infectious protein meals.	infection of mosquitoes is an essential step for the transmission of mosquito-borne arboviruses in nature. engorgement of infectious blood meals from viremic infected vertebrate hosts allows the entry of viruses and initiates infection of midgut epithelial cells. historically, the infection process of arboviruses in mosquitoes has been studied through the engorgement of mosquitoes from viremic laboratory animals or from artificial feeders containing blood mixed with viruses harvested from cell c ...	2017	28796799
zika virus replication in the mosquito culex quinquefasciatus in brazil.	zika virus (zikv) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. the virus is primarily transmitted by mosquito bite, although other routes of infection have been implicated in some cases. the aedes aegypti mosquito is considered to be the main vector to humans worldwide; however, there is evidence that other mosquito species, including culex quinquefasciatus, transmit the virus. to test the potential of cx ...	2017	28790458
sexual and vertical transmission of zika virus in anti-interferon receptor-treated rag1-deficient mice.	although zika virus (zikv) is primarily transmitted to humans by the aedes aegypti mosquito, human-to-human transmission has also been observed from males-to-females as well as mother-to-offspring. in the current study, we studied both sexual transmission (stx) and vertical transmission (vtx) of zikv using anti-ifnar1-treatment of rag1 (-/-) (air) mice. these mice have suppressed type i ifn responses and lack adaptive immune responses, leading to a prolonged infection prior to clinical disease. ...	2017	28775298
culex quinquefasciatus from areas with the highest incidence of microcephaly associated with zika virus infections in the northeast region of brazil are refractory to the virus.	zika virus (zikv) is widely distributed in brazil and the northeast region (ne) is the most affected zone, showing the highest incidence of microcephaly associated with zikv congenital infections worldwide. we report attempts to infect three populations of culex quinquefasciatus from severely affected sites in the ne and southeast region (se) of brazil with three strains of zikv isolated from these localities. an aedes aegypti population from the se was used as a positive control. all tested cx. ...	2017	28767975
linking only aedes aegypti with zika virus has world-wide public health implications.		2017	28736548
zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process.	zika disease has transformed into a serious global health problem due to the rapid spread of the arbovirus and alarming severity including congenital complications, microcephaly and guillain-barré syndrome. zika virus (zikv) is primarily transmitted to humans through the bite of an infective mosquito, with aedes aegypti being the main vector.	2017	28728607
asymptomatic transmission and the dynamics of zika infection.	following the 2013-14 outbreak in french polynesia, the zika virus (zikv) epidemic spread widely to many countries where aedes aegypti as the main transmitting vector is endemic. the lack of a second wave of zikv infection in most affected regions may suggest that a sufficiently high level of herd immunity was reached during the first wave. we developed an agent-based transmission model to investigate the role of asymptomatic infection on the likelihood of observing a second wave, while accounti ...	2017	28724972
american aedes vexans mosquitoes are competent vectors of zika virus.	abstractstarting in 2013-2014, the americas have experienced a massive outbreak of zika virus (zikv) which has now reached at least 49 countries. although most cases have occurred in south america and the caribbean, imported and autochthonous cases have occurred in the united states. aedes aegypti and aedes albopictus mosquitoes are known vectors of zikv. little is known about the potential for temperate aedes mosquitoes to transmit zikv. aedes vexans has a worldwide distribution, is highly abun ...	2017	28719283
early evidence for zika virus circulation among aedes aegypti mosquitoes, rio de janeiro, brazil.	during 2014-2016, we conducted mosquito-based zika virus surveillance in rio de janeiro, brazil. results suggest that zika virus was probably introduced into the area during may-november 2013 via multiple in-country sources. furthermore, our results strengthen the hypothesis that zika virus in the americas originated in brazil during october 2012-may 2013.	2017	28628464
a single substitution changes zika virus infectivity in mosquitoes.	zika virus (zikv) has caused outbreaks in the pacific and the americas. the mechanism underlying the recent zikv epidemic remains obscure. a recent study reveals that an amino acid substitution is associated with increased infectivity of zikv in the aedes aegypti mosquito.	2017	28610876
zika vector transmission risk in temperate australia: a vector competence study.	zika virus is an emerging pathogen of global importance. it has been responsible for recent outbreaks in the americas and in the pacific region. this study assessed five different mosquito species from the temperate climatic zone in australia and included aedes albopictus as a potentially invasive species.	2017	28599659
emerging infectious disease agents and blood safety in australia: spotlight on zika virus.	emerging infectious diseases (eids) are infectious diseases whose incidence has increased in humans in the past 20 years or could increase in the near future. eid agents may represent a threat to blood safety if they infect humans, cause a clinically significant illness, include an asymptomatic blood phase in the course of infection, and are transmissible by transfusion. eid agents are typically not well characterised, but there is a consensus that we can expect ongoing outbreaks. strategies to ...	2017	28566073
genomic epidemiology reveals multiple introductions of zika virus into the united states.	zika virus (zikv) is causing an unprecedented epidemic linked to severe congenital abnormalities. in july 2016, mosquito-borne zikv transmission was reported in the continental united states; since then, hundreds of locally acquired infections have been reported in florida. to gain insights into the timing, source, and likely route(s) of zikv introduction, we tracked the virus from its first detection in florida by sequencing zikv genomes from infected patients and aedes aegypti mosquitoes. we s ...	2017	28538723
de novo generation and characterization of new zika virus isolate using sequence data from a microcephaly case.	zika virus (zikv) has recently emerged and is the etiological agent of congenital zika syndrome (czs), a spectrum of congenital abnormalities arising from neural tissue infections in utero. herein, we describe the de novo generation of a new zikv isolate, zikvnatal, using a modified circular polymerase extension reaction protocol and sequence data obtained from a zikv-infected fetus with microcephaly. zikvnatal thus has no laboratory passage history and is unequivocally associated with czs. zikv ...	2017	28529976
outbreak of zika virus infection in singapore: an epidemiological, entomological, virological, and clinical analysis.	an outbreak of zika virus infection was detected in singapore in august, 2016. we report the first comprehensive analysis of a national response to an outbreak of zika virus infection in asia.	2017	28527892
eco-friendly and cost-effective ag nanocrystals fabricated using the leaf extract of habenaria plantaginea: toxicity on six mosquito vectors and four non-target species.	recently, the biofabrication of metal nanoparticles has gained wide interest owing to its inherent features such as swift, simplicity, eco-friendliness, and cheaper costs. different green-reducing agents led to the production of nanoparticles with varying toxicity on insects. in the current study, silver nanoparticles (agnps) were successfully synthesized using habenaria plantaginea leaf extract. ag nanoparticles were studied by uv-vis spectroscopy (uv-vis), x-ray diffraction (xrd), fourier tran ...	2017	28523613
evolutionary enhancement of zika virus infectivity in aedes aegypti mosquitoes.	zika virus (zikv) remained obscure until the recent explosive outbreaks in french polynesia (2013-2014) and south america (2015-2016). phylogenetic studies have shown that zikv has evolved into african and asian lineages. the asian lineage of zikv was responsible for the recent epidemics in the americas. however, the underlying mechanisms through which zikv rapidly and explosively spread from asia to the americas are unclear. non-structural protein 1 (ns1) facilitates flavivirus acquisition by m ...	2017	28514450
a location-specific spreadsheet for estimating zika risk and timing for zika vector surveillance, using us military facilities as an example.	local zika virus transmission in the united states involving one or both of the known vector species, aedes aegypti and ae. albopictus, is of major concern. to assist efforts to anticipate the risks of transmission, we developed an excel spreadsheet tool that uses vector and virus temperature thresholds, remotely sensed maximum temperature, and habitat suitability models to answer the questions: "is zika transmission likely here?" and "when should we conduct vector surveillance?" an example spre ...	2017	28511272
transmission incompetence of culex quinquefasciatus and culex pipiens pipiens from north america for zika virus.	abstractin late 2014, zika virus (zikv; flaviviridae, flavivirus) emerged as a significant arboviral disease threat in the western hemisphere. aedes aegypti and aedes albopictus have been considered the principal vectors of zikv in the new world due to viral isolation frequency and vector competence assessments. limited reports of culex transmission potential have highlighted the need for additional vector competence assessments of north american culex species. accordingly, north american culex ...	2017	28500817
potential of a northern population of aedes vexans (diptera: culicidae) to transmit zika virus.	zika virus is an emerging arbovirus of humans in the western hemisphere. with its potential spread into new geographical areas, it is important to define the vector competence of native mosquito species. we tested the vector competency of aedes vexans (meigen) from the lake agassiz plain of northwestern minnesota and northeastern north dakota. aedes aegypti (l.) was used as a positive control for comparison. mosquitoes were fed blood containing zika virus and 2 wk later were tested for viral inf ...	2017	28499036
a zika virus from america is more efficiently transmitted than an asian virus by aedes aegypti mosquitoes from asia.	zika is a mosquito-borne disease associated with neurological disorders that causes an on-going pandemic. the first outbreak was recorded in micronesia in 2007, then in french polynesia in 2014 from which it spread to south america in 2015 and ignited a widespread epidemic. interestingly, zika outbreaks in asia remained of moderate intensity although the virus is circulating. to understand these epidemiological variations, we investigated the entomological determinants of zikv transmission in as ...	2017	28450714
vector competence and transovarial transmission of two aedes aegypti strains to zika virus.	zika virus (zikv) has become a serious threat to global health since the outbreak in brazil in 2015. additional chinese cases have continuously been reported since the first case of laboratory-confirmed zikv infection in china on 6 february 2016. aedes aegypti is the most important vector for zikv. this study shows that two strains from china exhibit high levels of midgut infection and highly disseminated infection of salivary glands and ovaries. both strains can transmit zikv to infant mice bit ...	2017	28442754
effects of zika virus strain and aedes mosquito species on vector competence.	in the western hemisphere, zika virus is thought to be transmitted primarily by aedes aegypti mosquitoes. to determine the extent to which ae. albopictus mosquitoes from the united states are capable of transmitting zika virus and the influence of virus dose, virus strain, and mosquito species on vector competence, we evaluated multiple doses of representative zika virus strains in ae. aegypti and ae. albopictus mosquitoes. virus preparation (fresh vs. frozen) significantly affected virus infect ...	2017	28430564
competence of aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes as zika virus vectors, china.	in china, the prevention and control of zika virus disease has been a public health threat since the first imported case was reported in february 2016. to determine the vector competence of potential vector mosquito species, we experimentally infected aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes and determined infection rates, dissemination rates, and transmission rates. we found the highest vector competence for the imported zika virus in ae. aegypti mosquitoes, some sus ...	2017	28430562
evidence of zika virus rna fragments in aedes albopictus (diptera: culicidae) field-collected eggs from camaçari, bahia, brazil.	a major mosquito-borne viral disease outbreak caused by zika virus (zikv) occurred in bahia, brazil, in 2015, largely due to transmission by the mosquito, aedes aegypti (l.). detecting zikv in field samples of ae. aegypti has proven problematic in some locations, suggesting other mosquito species might be contributing to the spread of zikv. in this study, several (five) adult aedes albopictus (skuse) mosquitoes that emerged from a 2015 field collection of eggs from camaçari, bahia, brazil, were ...	2017	28419254
chemosensory responses to the repellent nepeta essential oil and its major component nepetalactone by aedes aegypti (diptera: culicidae), a vector of zika virus.	nepeta essential oil (neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. however, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito aedes aegypti (l.), an important vector of zika virus, were poorly understood. here we show that neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female ae. aegypti. a gustatory rece ...	2017	28407077
quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection.	zika virus (zikv) is an emerging arbovirus belonging to the genus flavivirus of the family flaviviridae during replication processes, flavivirus manipulates host cell systems to facilitate its replication, while the host cells activate antiviral responses. identification of host proteins involved in the flavivirus replication process may lead to the discovery of antiviral targets. the mosquitoes aedes aegypti and aedes albopictus are epidemiologically important vectors for zikv, and effective re ...	2017	28404849
persistence of toxic activity of fermentation extracts from bacillus thuringiensis var. israelensis after more than three decades of storage.	this study was carried out to determine the persistence of toxicity of fermentation extracts of bacillus thuringiensis var. israelensis after more than three decades of storage. for this purpose, a population of aedes aegypti was established. the mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified who protocol. the extracts with the highest mortality rate were determined in triplicat ...	2017	29358951
a small-scale investigation into the effect of a larvicidal oil on oviposition site preference by aedes aegypti.	larvicidal oils can be used to control immature aedes aegypti and other pestiferous mosquitoes. to test whether oil-based larvicides also act as oviposition deterrents, indoor and semi-field trials were conducted at the navy entomology center of excellence in jacksonville, fl. in both studies, treatment cages consisted of oviposition cups lined with seed germination paper as an oviposition substrate and filled with 1-wk-old southern live oak ( quercus virginiana) leaf litter-infused water. treat ...	2017	29369032
comparison of adult mosquito abundance from biogents-2 sentinel and biogents gravid aedes traps in northeastern florida.	we compared the efficacy of the biogents gravid aedes trap (bg-gat) and biogents-2 sentinel (bg2s) trap to assess differences in adult mosquito collection rates between these 2 mosquito surveillance methods in northeastern florida. species diversity of trap collections was significantly greater for bg2s than for bg-gat, with 14 mosquito species collected from the former trap and 4 species from the latter. aedes aegypti and culex quinquefasciatus were the 2 predominant species collected in both t ...	2017	29369031
bg-sentinel™ trap efficacy as a component of proof-of-concept for push-pull control strategy for dengue vector mosquitoes.	the efficacy of the bg-sentinel™ (bgs) trap as a "pull" component of a "push-pull" system (pps) for management of the dengue vector, aedes aegypti, was evaluated using local households in pu tuey, kanchanaburi, thailand. the pull component was the concluding phase of a 3-part investigation using a pps combination spatial repellent (sr) and bgs trap to capture adult vector mosquitoes. two sentinel households were selected for evaluation of bgs trap efficacy based on the highest pretrial indoor re ...	2017	29369029
laboratory evaluation of synthetic blends of l-(+)-lactic acid, ammonia, and ketones as potential attractants for aedes aegypti.	attraction of aedes aegypti to various binary, trinary, and quaternary blends of lactic acid and ketones with or without ammonia was studied using a dual choice olfactometer. a dose dependent attraction was observed in cases of single compounds where cyclopentanone attracted the highest percentage (36.9 ± 1.8%) of ae. aegypti when tested alone. no significant difference was observed between the attraction levels of trinary and binary blends of lactic acid and acetone or butanone when tested agai ...	2017	29369028
synergistic effect of fertilizer and plant material combinations on the development of aedes aegypti (diptera: culicidae) and anopheles gambiae (diptera: culicidae) mosquitoes.	chemical fertilizers are used everywhere, which often pollute the breeding sites of mosquitoes. in this laboratory study, the consequences on aedes aegypti (l.) (diptera: culicidae) and anopheles gambiae (giles) (diptera: culicidae) of water-containing plant matter (pm) alone, or in association with an npk type of fertilizer (pm+npk), were evaluated. to obtain a 20% imaginal emergence of an. gambiae (iet20), the bioassays carried out with pm have evidenced that its larvae need four times as much ...	2017	29309617
dengue and yellow fever virus vectors: seasonal abundance, diversity and resting preferences in three kenyan cities.	the transmission patterns of dengue (denv) and yellow fever (yfv) viruses, especially in urban settings, are influenced by aedes (stegomyia) mosquito abundance and behavior. despite recurrent dengue outbreaks on the kenyan coast, these parameters remain poorly defined in this and other areas of contrasting dengue endemicity in kenya. in assessing the transmission risk of denv/yfv in three kenyan cities, we determined adult abundance and resting habits of potential aedes (stegomyia) vectors in ki ...	2017	29284522
genetically modified aedes aegypti to control dengue: a review.	dengue is an acute infectious disease of viral etiology characterized by lymphadenopathy, leucopenia, headache, biphasic fever, pain in various parts of the body, rashes, and extreme physical weakness. it is a vector-borne disease caused by a positive-stranded rna virus of the family flaviviridae, genus flavivirus. dengue inflicts a significant health, economic, and social burden on populations of endemic areas. dengue virus is transmitted to humans by the mosquito vector aedes aegypti. vaccines ...	2017	29283327
family level variation in wolbachia-mediated dengue virus blocking in aedes aegypti.	the mosquito vector aedes aegypti is responsible for transmitting a range of arboviruses including dengue (denv) and zika (zikv). the global reach of these viruses is increasing due to an expansion of the mosquito's geographic range and increasing urbanization and human travel. vector control remains the primary means for limiting these diseases. wolbachia pipientis is an endosymbiotic bacterium of insects that has the ability to block the replication of pathogens, including flaviviruses such as ...	2017	29282144
african and asian zika virus isolates display phenotypic differences both in vitro and in vivo.	zika virus (zikv) is a mosquito-borne member of the genus flavivirus that has emerged since 2007 to cause outbreaks in africa, asia, oceania, and most recently, in the americas. here, we used an isolate history as well as genetic and phylogenetic analyses to characterize three low-passage isolates representing african (ard 41525) and asian (cpc-0740, sv0127-14) lineages to investigate the potential phenotypic differences in vitro and in vivo. the african isolate displayed a large plaque phenotyp ...	2017	29280428
altitudinal distribution of aedes indices during dry season in the dengue endemic area of central java, indonesia	aedes mosquitoes, mainly aedes aegypti and aedes albopictus, are the primary and secondary vectors of dengue viruses in indonesia, with transmission occurring by sucking blood. the density of the vectors is influenced by season and rainfall, but limited by altitude. the aim of the study is to describe the density and distribution of dengue vectors during the dry season based on the altitudes of recent dengue cases in five regencies of central java province, indonesia. mosquito larvae and pupae w ...	2017	29274215
zika virus: an emerging infectious disease with serious perinatal and neurologic complications.	zika virus (zikv) is a flavivirus that is primarily transmitted by aedes aegypti, the mosquito vector also important in the transmission of the flaviviruses responsible for dengue fever, yellow fever and chikungunya. due to occurrence in the same geographic regions, serological cross-reactivity, and similar, albeit often less severe clinical manifestations as dengue and chikungunya infections, zikv infection likely went undetected and/or misdiagnosed for many years. zikv is somewhat unique among ...	2017	29273403
history and emergence of zika virus.	zika virus was discovered in east africa in 1947 by the rockefeller foundation during investigations on the ecology of yellow fever. although it was subsequently shown to have widespread distribution in africa and asia, it was not known to cause epidemics until 2007. this paper describes the history of the virus discovery, emergence and evolution as an epidemic virus, and the its evolving clinical spectrum.	2017	29267917
zika virus mosquito vectors: competence, biology, and vector control.	zika virus (zikv) (flaviviridae, flavivirus) has become one of the most medically important mosquito-borne viruses because of its ability to cause microcephaly in utero and guillain-barré syndrome in adults. this virus emerged from its sylvatic cycle in africa to cause an outbreak in yap, federated states of micronesia in 2007, french polynesia in 2014, and most recently south america in 2015. the rapid expansion of zikv in the americas largely has been due to the biology and behavior of its vec ...	2017	29267910
chikungunya: molecular aspects, clinical outcomes and pathogenesis.	the alarming worldwide emergence of the chikungunya virus began in the last decade. since the first autochthonous transmission in mexico in november of 2014, the virus has spread throughout the country, resulted in multiple outbreaks. this virus produces an acute and self-limiting disease characterized by fever, polyarthralgia, myalgia, exanthema, and general malaise. it is transmitted to humans by the bite of aedes aegypti and a. albopictus mosquitoes. the fact that the clinical presentation is ...	2017	29265115
characteristics of aedes aegypti adult mosquitoes in rural and urban areas of western and coastal kenya.	aedes aegypti is the main vector for yellow fever, dengue, chikungunya and zika viruses. recent outbreaks of dengue and chikungunya have been reported in kenya. presence and abundance of this vector is associated with the risk for the occurrence and transmission of these diseases. this study aimed to characterize the presence and abundance of ae. aegypti adult mosquitoes from rural and urban sites in western and coastal regions of kenya. presence and abundance of ae. aegypti adult mosquitoes wer ...	2017	29261766
individual co-variation between viral rna load and gene expression reveals novel host factors during early dengue virus infection of the aedes aegypti midgut.	dengue virus (denv) causes more human infections than any other mosquito-borne virus. the current lack of antiviral strategies has prompted genome-wide screens for host genes that are required for denv infectivity. earlier transcriptomic studies that identified denv host factors in the primary vector aedes aegypti used inbred laboratory colonies and/or pools of mosquitoes that erase individual variation. here, we performed transcriptome sequencing on individual midguts in a field-derived ae. aeg ...	2017	29261661
assessment of maldi-tof mass spectrometry for filariae detection in aedes aegypti mosquitoes.	matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is an emerging tool for routine identification of bacteria, archaea and fungi. it has also been recently applied as an accurate approach for arthropod identification. preliminary studies have shown that the maldi-tof ms was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and plasmodium parasites, respectively. the aim of the present study was to test the efficien ...	2017	29261659
origin of a high-latitude population of aedes aegypti in washington, dc.	an overwintering population of aedes aegypti has been documented in the capitol hill neighborhood of washington, dc, since 2011. mitochondrial cytochrome oxidase i (mtcoi) sequence data presented in a previous study traced the origin to the new world. here, we use microsatellite and snp chip data along with more mitochondrial dna (mtdna) sequences on washington ae. aegypti samples and samples from potential sources to further narrow the origin of this population. genetically, washington ae. aegy ...	2017	29260658
from ground pools to treeholes: convergent evolution of habitat and phenotype in aedes mosquitoes.	invasive mosquito species are responsible for millions of vector-borne disease cases annually. the global invasive success of aedes mosquitoes such as aedes aegypti and aedes albopictus has relied on the human transport of immature stages in container habitats. however, despite the importance of these mosquitoes and this ecological specialization to their widespread dispersal, evolution of habitat specialization in this group has remained largely unstudied. we use comparative methods to evaluate ...	2017	29258425
zika virus exhibits lineage-specific phenotypes in cell culture, in aedes aegypti mosquitoes, and in an embryo model.	zika virus (zikv) has quietly circulated in africa and southeast asia for the past 65 years. however, the recent zikv epidemic in the americas propelled this mosquito-borne virus to the forefront of flavivirus research. based on historical evidence, zikv infections in africa were sporadic and caused mild symptoms such as fever, skin rash, and general malaise. in contrast, recent asian-lineage zikv infections in the pacific islands and the americas are linked to birth defects and neurological dis ...	2017	29258204
seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan.	to find out the variations in larval and adult density of aedes aegypti in different seasons under different ecological conditions.	2017	29256519
social-ecological factors and preventive actions decrease the risk of dengue infection at the household-level: results from a prospective dengue surveillance study in machala, ecuador.	in ecuador, dengue virus (denv) infections transmitted by the aedes aegypti mosquito are among the greatest public health concerns in urban coastal communities. community- and household-level vector control is the principal means of controlling disease outbreaks. this study aimed to assess the impact of knowledge, attitudes, and practices (kaps) and social-ecological factors on the presence or absence of denv infections in the household.	2017	29253873
an approach of the exact linearization techniques to analysis of population dynamics of the mosquito aedes aegypti.	dengue fever, chikungunya and zika are caused by different viruses and mainly transmitted by aedes aegypti mosquitoes. these diseases have received special attention of public health officials due to the large number of infected people in tropical and subtropical countries and the possible sequels that those diseases can cause. in severe cases, the infection can have devastating effects, affecting the central nervous system, muscles, brain and respiratory system, often resulting in death. vaccin ...	2017	29253493
aedes aegypti resistance development to commonly used insecticides in jakarta, indonesia.	aedes aegypti is the primary vector of various relevant arthropod-borne viral infectious diseases worldwide. the mosquito control is still mainly performed by using insecticides but their effectiveness is increasingly questioned nowadays. we here conducted a study on ae. aegypti resistance development towards several commonly used insecticides in the capital city of jakarta, indonesia. in order to achieve this goal, ae. aegypti eggs from jakarta were collected with ovitraps and hatched in the in ...	2017	29253003
analytical report of the 2016 dengue outbreak in córdoba city, argentina.	after elimination of the aedes aegypti vector in south america in the 1960s, dengue outbreaks started to reoccur during the 1990s; strongly in argentina since 1998. in 2016, córdoba city had the largest dengue outbreak in its history. in this article we report this outbreak including spatio-temporal analysis of cases and vectors in the city. a total of 653 dengue cases were recorded by the laboratory-based dengue surveillance system and georeferenced by their residential addresses. case maps wer ...	2017	29239555
assessing natural infection with zika virus in the southern house mosquito, culex quinquefasciatus, during 2016 in puerto rico.	the epidemic of zika in the western hemisphere has led to intense investigations of all species important in the transmission of zika virus (zikv), including putative mosquito vectors. although evidence points to stegomyia (= aedes) (diptera: culicidae) mosquitoes as the primary vectors in nature among humans, there remains the possibility that other common mosquito species may be implicated in the rapid spread of the virus. herein, field-caught culex quinquefasciatus (diptera: culicidae) collec ...	2017	29239003
aedes aegypti breeding site in an underground rainwater reservoir: a warning.	we describe the discovery of aedes aegypti underground breeding site in the pinheiros neighborhood of são paulo, sp, during an entomological survey program performed in 2016. even with intense surveillance and vector control, large numbers of mosquitoes were present in this area. a detailed investigation allowed for the detection of ae. aegypti in an underground reservoir used for rainwater storage. after the implementation of protection screens in the accesses, the presence of the vector was no ...	2017	29236877
detection of zika virus in aedes mosquitoes from mexico.	we report on the results of an entomovirological surveillance system of aedes populations performed by the ministry of health of the central state of san luis potosí, mexico.	2017	29232453
efficacy of 13 commercial household aerosol insecticides against aedes aegypti (diptera: culicidae) from morelos, mexico.	in mexico, aedes aegypti (l.) (diptera: culicidae) is the primary vector of dengue, zika, and chikungunya viruses. control programs include community participation using personal protection such as household aerosol insecticides. in both, urban or rural areas, the use of aerosol insecticides is a common practice to avoiding mosquito biting. thus, information on the efficacy of commercial products must be available. this study reports the efficacy of 13 household aerosol insecticides against ae. ...	2017	29228197
the first detected airline introductions of yellow fever mosquitoes (aedes aegypti) to europe, at schiphol international airport, the netherlands.	air-borne introduction of exotic mosquitoes to schiphol airport in the netherlands has been considered plausible based upon findings of mosquitoes in aircraft cabins during 2008, 2010 and 2011. beginning in 2013, surveillance efforts at schiphol had focused on promptly detecting accidental introductions at the airport facilities in order to quickly react and avoid temporary proliferation or establishment of mosquito populations, identify the origin of the introductions, and avoid potential trans ...	2017	29221490