Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| identification of functional enolase genes of the silkworm bombyx mori from public databases with a combination of dry and wet bench processes. | various insect species have been added to genomic databases over the years. thus, researchers can easily obtain online genomic information on invertebrates and insects. however, many incorrectly annotated genes are included in these databases, which can prevent the correct interpretation of subsequent functional analyses. to address this problem, we used a combination of dry and wet bench processes to select functional genes from public databases. | 2017 | 28086791 |
| application of the analytic hierarchy approach to the risk assessment of zika virus disease transmission in guangdong province, china. | an international spread of zika virus (zikv) infection has attracted global attention in 2015. the infection also affected guangdong province, which is located in southern china. multiple factors, including frequent communication with south america and southeast asia, suitable climate (sub-tropical) for the habitat of aedes species, may increase the risk of zikv disease transmission in this region. | 2017 | 28086897 |
| functional characterization of the active mutator-like transposable element, muta1 from the mosquito aedes aegypti. | mutator-like transposable elements (mules) are widespread with members in fungi, plants, and animals. most of the research on the mule superfamily has focused on plant mules where they were discovered and where some are extremely active and have significant impact on genome structure. the maize mudr element has been widely used as a tool for both forward and reverse genetic studies because of its high transposition rate and preference for targeting genic regions. however, despite being widesprea ... | 2017 | 28096902 |
| juvenile hormone biosynthesis in adult blattella germanica requires nuclear receptors seven-up and ftz-f1. | in insects, the transition from juvenile development to the adult stage is controlled by juvenile hormone (jh) synthesized from the corpora allata (ca) glands. whereas a jh-free period during the last juvenile instar triggers metamorphosis and the end of the growth period, the reappearance of this hormone after the imaginal molt marks the onset of reproductive adulthood. despite the importance of such transition, the regulatory mechanism that controls it remains mostly unknown. here, using the h ... | 2017 | 28074850 |
| emergence of epidemic zika virus transmission and congenital zika syndrome: are recently evolved traits to blame? | the mechanisms responsible for the dramatic emergence of zika virus (zikv), accompanied by congenital zika syndrome and guillain-barré syndrome (gbs), remain unclear. however, two hypotheses are prominent: (i) evolution for enhanced urban transmission via adaptation to mosquito vectors, or for enhanced human infection to increase amplification, or (ii) the stochastic introduction of zikv into large, naive human populations in regions with abundant aedes aegypti populations, leading to enough rar ... | 2017 | 28074023 |
| fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression. | diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. diapause-destined insects store large amounts of fat when preparing for diapause. however, the extent to which these accumulated fat reserves influence diapause remains unclear. to address this question, we investigated the function of fatty acid synthase (fas), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause prepar ... | 2017 | 28071706 |
| dengue in latin america: systematic review of molecular epidemiological trends. | dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (denv-1, -2, -3 or -4). a literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in latin america, including the caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research ... | 2017 | 28068335 |
| proteomic analysis of cry2aa-binding proteins and their receptor function in spodoptera exigua. | the bacterium bacillus thuringiensis produces crystal (cry) proteins that are toxic to a diverse range of insects. transgenic crops that produce bt cry proteins are grown worldwide because of their improved resistance to insect pests. although bt "pyramid" cotton that produces both cry1a and cry2a is predicted to be more resistant to several lepidopteran pests, including spodoptera exigua, than plants that produce cry1ac alone, the mechanisms responsible for the toxicity of cry2aa in s. exigua a ... | 2017 | 28067269 |
| wolbachia infections in aedes aegypti differ markedly in their response to cyclical heat stress. | aedes aegypti mosquitoes infected with wolbachia bacteria are currently being released for arbovirus suppression around the world. their potential to invade populations and persist will depend on interactions with environmental conditions, particularly as larvae are often exposed to fluctuating and extreme temperatures in the field. we reared ae. aegypti larvae infected with different types of wolbachia (wmel, walbb and wmelpop-cla) under diurnal cyclical temperatures. rearing wmel and wmelpop-c ... | 2017 | 28056065 |
| the dangers of accepting a single diagnosis: case report of concurrent plasmodium knowlesi malaria and dengue infection. | dengue and malaria are two common, mosquito-borne infections, which may lead to mortality if not managed properly. concurrent infections of dengue and malaria are rare due to the different habitats of its vectors and activities of different carrier mosquitoes. the first case reported was in 2005. since then, several concurrent infections have been reported between the dengue virus (denv) and the malaria protozoans, plasmodium falciparum and plasmodium vivax. symptoms of each infection may be mas ... | 2017 | 28049485 |
| pterodon emarginatus oleoresin-based nanoemulsion as a promising tool for culex quinquefasciatus (diptera: culicidae) control. | preparation of nanoformulations using natural products as bioactive substances is considered very promising for innovative larvicidal agents. on this context, oil in water nanoemulsions develop a main role, since they satisfactorily disperse poor-water soluble substances, such as herbal oils, in aqueous media. pterodon emarginatus, popularly known as sucupira, has a promising bioactive oleoresin. however, to our knowledge, no previous studies were carried out to evaluate its potential against cu ... | 2017 | 28049483 |
| the physical genome mapping of anopheles albimanus corrected scaffold misassemblies and identified interarm rearrangements in genus anopheles. | the genome of the neotropical malaria vector anopheles albimanus was sequenced as part of the 16 anopheles genomes project published in 2015. the draft assembly of this species consisted of 204 scaffolds with an n50 scaffold size of 18.1 mb and a total assembly size of 170.5 mb. it was among the smallest genomes with the longest scaffolds in the 16 anopheles species cluster, making an. albimanus the logical choice for anchoring the genome assembly to chromosomes. in this study, we developed a hi ... | 2017 | 27821634 |
| the transcriptome of the mosquito aedes fluviatilis (diptera: culicidae), and transcriptional changes associated with its native wolbachia infection. | wolbachia is a bacterial endosymbiont that naturally infects a wide range of insect species, and causes drastic changes to host biology. stable infections of wolbachia in mosquitoes can inhibit infection with medically important pathogens such as dengue virus and malaria-causing plasmodium parasites. however, some native wolbachia strains can enhance infection with certain pathogens, as is the case for the mosquito aedes fluviatilis, where infection with plasmodium gallinaceum is enhanced by the ... | 2017 | 28049478 |
| argonaute 2 suppresses japanese encephalitis virus infection in aedes aegypti. | there are three main innate immune mechanisms against viruses in mosquitoes. infection with the flavivirus dengue virus is controlled by rna interference (rnai) and the jak-stat and toll signaling pathways. this study showed that another flavivirus, japanese encephalitis virus (jev), did not invade the salivary glands of aedes aegypti and that this may be a result of the innate immune resistance to the virus. argonaute 2 (ago2) plays a critical role in the rnai pathway. to understand the mechani ... | 2017 | 27169949 |
| molecular identification of aedes aegypti mosquitoes from pilani region of rajasthan, india. | aedes aegypti is the most important vector of dengue virus infection in humans worldwide. accurate identification and colonization are the essential requirements to understand vector biology as well as its diseases transmission potential. in this study, we have used molecular approaches for the identification of ae. aegypti mosquitoes that were collected from the pilani region of rajasthan, india methods: field collected mosquito larvae were colonized under laboratory conditions. conserved genet ... | 2017 | 27353585 |
| microbiota activates imd pathway and limits sindbis infection in aedes aegypti. | aedes aegypti is the main vector of important arboviruses such as dengue, zika and chikungunya. during infections mosquitoes can activate the immune pathways toll, imd and jak/stat to limit pathogen replication. | 2017 | 28231846 |
| the evolutionary divergence of stat transcription factor in different anopheles species. | anopheles mosquito transmits plasmodium, the malaria causing parasite. different species of anopheles mosquito dominate in a particular geographical location and are capable of transmitting specific strains of plasmodium. it is important to understand the biology of different anophelines to control the parasite transmission. stat is an evolutionary conserved transcription factor that regulates the parasite development in african malaria vector anopheles gambiae. unlike drosophila and aedes aegyp ... | 2017 | 27664587 |
| molecular expression of aquaporin mrnas in the northern house mosquito, culex pipiens. | aquaporins (aqps) are a family of membrane-bound proteins, originally described as water-channels, that broadly exist in prokaryotic and eukaryotic cells. however, some aqps can transport small molecules (e.g. urea, glycerol) along with or preferentially to water. previous work in the mosquitoes aedes aegypti and anopheles gambiae have characterized the molecular expression of one or more aqp genes and shown that they are involved in water homeostasis after blood feeding, tolerance to dehydratio ... | 2017 | 27773635 |
| larvicidal and growth-inhibitory activity of entomopathogenic bacteria culture fluids against aedes aegypti (diptera: culicidae). | dengue, chikungunya, and zika are important vector-borne diseases, and aedes aegypti l. is their main transmitter. as the disease management is mainly based on mosquito control strategies, the search for alternative and cost-effective approaches is ongoing. the gram-negative bacteria xenorhabdus nematophila and photorhabdus luminescens are symbiotically associated with entomopathogenic nematodes and are highly pathogenic for insect larvae. after we have recently confirmed the toxicity of these b ... | 2017 | 28062794 |
| artesunate-tafenoquine combination therapy promotes clearance and abrogates transmission of the avian malaria parasite plasmodium gallinaceum. | clinical manifestations of malaria infection in vertebrate hosts arise from the multiplication of the asexual stage parasites in the blood, while the gametocytes are responsible for the transmission of the disease. antimalarial drugs that target the blood stage parasites and transmissible gametocytes are rare, but are essentially needed for the effective control of malaria and for limiting the spread of resistance. artemisinin and its derivatives are the current first-line antimalarials that are ... | 2017 | 28043395 |
| toxicity of herbal extracts used in ethno-veterinary medicine and green-encapsulated zno nanoparticles against aedes aegypti and microbial pathogens. | dengue and chikungunya are arboviral diseases mainly vectored by the mosquito aedes aegypti. presently, there is no treatment for these viral diseases and their prevention is still based on vector control measures. nanopesticides fabricated using herbal extracts as reducing and capping agents currently represent an excellent platform for pest control. in this scenario, the present study assessed the acute toxicity of seven plants employed in ethno-veterinary medicine of southern india, as well a ... | 2017 | 28389893 |
| pseudomonas aeruginosa kun2, extracellular toxins-a potential source for the control of dengue vector. | dengue fever is one of the serious health disease transmitted by aedes spp mosquitoes. the incidence of dengue has increased dramatically around the world in recent decades. vector control is one of the important strategies practiced for the control of dengue fever. the emergence of resistance among vectors against the existing insecticides has raised new challenges. the aim of the present study was to identify the larvicidal activity of extracellular toxins from pseudomonas spp for the control ... | 2017 | 27353579 |
| n-glycosylation influences the catalytic activity of mosquito α-glucosidases associated with susceptibility or refractoriness to lysinibacillus sphaericus. | cqm1 and aam1 are α-glucosidases (ec 3.2.1.20) expressed in culex quinquefasciatus and aedes aegypti larvae midgut, respectively. these orthologs share high sequence similarity but while cqm1 acts as a receptor for the binary (bin) insecticidal toxin from lysinibacillus sphaericus, aam1 does not bind the toxin, rendering ae. aegypti refractory to this bacterium. aam1 is heavily glycosylated, contrasting to cqm1, but little is known regarding how glycosylation impacts on its function. this study ... | 2017 | 28017798 |
| mosgctl-7, a c-type lectin protein, mediates japanese encephalitis virus infection in mosquitoes. | japanese encephalitis virus (jev) is an arthropod-borne flavivirus prevalent in asia and the western pacific, and is the leading cause of viral encephalitis. jev is maintained in a transmission cycle between mosquitoes and vertebrate hosts, but the molecular mechanisms by which the mosquito vector participates in transmission are unclear. we investigated the expression of all c-type lectins during jev infection in aedes aegypti. c-type lectin mosgctl-7 (aael002524) was significantly up-regulated ... | 2017 | 28250133 |
| first case of zika virus infection in a hiv+ patient. | zika virus is an enveloped, single-stranded rna virus that belongs to the flavivirus genre. as with arbovirus, it is transmitted by arthropods (mosquitoes), both aedes aegypti (urban) and albopictus (rural). zika virus was first isolated in 1947 from monkeys in uganda. the first human cases were reported in 1952 in east africa. outbreaks were reported in micronesia (2007), polynesia (2013), and chile (2014). both diagnostic procedures and therapeutics are poorly developed. serological tests cros ... | 2017 | 27232506 |
| chemical composition and in vitro antioxidant, cytotoxic, antimicrobial, and larvicidal activities of the essential oil of mentha piperita l. (lamiaceae). | the essential oil was obtained by hydrodistillation and the identification and quantification of components were achieved with the use of gc-ms analysis. the antioxidant activity was evaluated by the method of sequestration of dpph. essential oils were used for study the cytotoxic front larvae of artemia salina. in the evaluation of the antimicrobial activity of essential oils, we employed the disk-diffusion method. the potential larvicide in mosquito larvae of the third stage of development of ... | 2017 | 28116346 |
| transcriptome sequencing reveals large-scale changes in axenic aedes aegypti larvae. | mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. we previously reported that aedes aegypti larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only escherichia coli develop very similarly into adults, whereas axenic larvae never molt and die as first instars. in this study, we extended these findings by first comparing the growth and abundance of bacteria in conventional, gnotobiotic, and axenic larvae during ... | 2017 | 28060822 |
| potential high-risk areas for zika virus transmission in the contiguous united states. | to understand where transmission of zika virus has the highest likelihood to occur in the contiguous united states with regard to its transmission both sexually and via aedes aegypti mosquito bites. | 2017 | 28323468 |
| development of dirofilaria immitis and dirofilaria repens in aedes japonicus and aedes geniculatus. | the mosquito-borne filarial nematodes dirofilaria immitis and dirofilaria repens primarily affect dogs but also cats, causing heartworm disease or subcutaneous dirofilariosis, respectively, and both may also cause zoonotic diseases in humans. several mosquito species have been reported as competent vectors for these nematodes, but no data are available for the invasive mosquito species aedes japonicus (theobald, 1901). the objective of this study was to describe the development of both d. immiti ... | 2017 | 28219407 |
| predicting arboviral disease emergence using bayesian networks: a case study of dengue virus in western australia. | a bayesian belief network (bbn) for assessing the potential risk of dengue virus emergence and distribution in western australia (wa) is presented and used to identify possible hotspots of dengue outbreaks in summer and winter. the model assesses the probabilities of two kinds of events which must take place before an outbreak can occur: (1) introduction of the virus and mosquito vectors to places where human population densities are high; and (2) vector population growth rates as influenced by ... | 2017 | 27620510 |
| an analysis of the potential impact of climate change on dengue transmission in the southeastern united states. | dengue fever, caused by a mosquito-transmitted virus, is an increasing health concern in the americas. meteorological variables such as temperature and precipitation can affect disease distribution and abundance through biophysical impacts on the vector and on the virus. such tightly coupled links may facilitate further spread of dengue fever under a changing climate. in the southeastern united states, the dengue vector is widely established and exists on the current fringe of dengue transmissio ... | 2017 | 27713106 |
| magnetic nanoparticles are highly toxic to chloroquine-resistant plasmodium falciparum, dengue virus (den-2), and their mosquito vectors. | a main challenge in parasitology is the development of reliable tools to prevent or treat mosquito-borne diseases. we investigated the toxicity of magnetic nanoparticles (mnp) produced by magnetospirillum gryphiswaldense (strain msr-1) on chloroquine-resistant (cq-r) and sensitive (cq-s) plasmodium falciparum, dengue virus (den-2), and two of their main vectors, anopheles stephensi and aedes aegypti, respectively. mnp were studied by fourier-transform infrared spectroscopy and transmission elect ... | 2017 | 27815736 |
| the dna chaperone hmgb1 potentiates the transcriptional activity of rel1a in the mosquito aedes aegypti. | high mobility group protein 1 (hmgb1) is a non-histone, chromatin-associated nuclear protein that functions in regulating eukaryotic gene expression. we investigated the influence and mechanism of action of aedes aegypti hmgb1 (aahmgb1) on mosquito rel1a-mediated transcription from target gene promoters. the dna-binding domain (rhd) of aarel1a was bacterially expressed and purified, and aahmgb1 dramatically enhanced rhd binding to consensus nf-kb/rel dna response elements. luciferase reporter an ... | 2017 | 27867076 |
| zika virus and neuroscience: the need for a translational collaboration. | zika virus (zikv) has become a major challenge for scientists and health agencies. zikv's involvement with human fetal microcephaly and guillain-barré syndrome and its transmission through aedes africanus and aedes aegypti mosquitos highlighted the epidemiological and neurological risks associated to zikv infection. in 2013, zikv arrives in brazil but the first outbreak in the country was reported in 2015. here, we used the web of science as a search tool for comparing the evolution of world and ... | 2017 | 28185126 |
| a heparin-functionalized carbon nanotube-based affinity biosensor for dengue virus. | dengue virus is an arthropod-borne virus transmitted primarily by aedes mosquitos and is major cause of disease in tropical and subtropical regions. colloquially known as dengue fever, infection can cause hemorrhagic disorders and death in humans and non-human primates. we report a novel electronic biosensor based on a single-walled carbon nanotube network chemiresistive transducer that is functionalized with heparin for low-cost, label-free, ultra-sensitive, and rapid detection of whole dengue ... | 2017 | 28152487 |
| dengue virus serotype 2 infection alters midgut and carcass gene expression in the asian tiger mosquito, aedes albopictus. | the asian tiger mosquito, aedes albopictus is currently an important vector for dengue, chikungunya and zika virus, and its role in transmission of arthropod-borne viruses (arboviruses) may increase in the future due to its ability to colonize temperate regions. in contrast to aedes aegypti, the dominant vector of dengue, chikungunya and zika virus, genetic responses of ae. albopictus upon infection with an arbovirus are not well characterized. here we present a study of the changes in transcrip ... | 2017 | 28152011 |
| superinfection interference between dengue-2 and dengue-4 viruses in aedes aegypti mosquitoes. | dengue virus consists of four antigenically distinct serotypes (denv 1-4) that are transmitted to humans by aedes aegypti and aedes albopictus mosquitoes. in many dengue-endemic regions, co-circulation of two or more denv serotypes is fairly common increasing the likelihood for exposure of the two vectors to multiple serotypes. we used a model system of denv-2 and denv-4 to investigate how prior exposure of aedes aegypti to one denv serotype affects its susceptibility to another serotype. | 2017 | 28150899 |
| the transovarial transmission in the dynamics of dengue infection: epidemiological implications and thresholds. | the anthropophilic and peridomestic female mosquito aedes aegypti bites humans to suck blood to maturate fertilized eggs, during which dengue virus can be spread between mosquito and human populations. besides this route of transmission, there is a possibility of dengue virus being passed directly to offspring through transovarial (or vertical) transmission. the effects of both horizontal and transovarial transmission routes on the dengue virus transmission are assessed by mathematical modeling. ... | 2017 | 28131789 |
| recent advances in the identification of the host factors involved in dengue virus replication. | dengue virus (denv) belongs to the genus flavivirus of the family flaviviridae and it is primarily transmitted via aedes aegypti and aedes albopictus mosquitoes. the life cycle of denv includes attachment, endocytosis, protein translation, rna synthesis, assembly, egress, and maturation. recent researches have indicated that a variety of host factors, including cellular proteins and micrornas, positively or negatively regulate the denv replication process. this review summarizes the latest findi ... | 2017 | 28124222 |
| aedes aegypti (diptera: culicidae) longevity and differential emergence of dengue fever in two cities in sonora, mexico. | dengue virus, primarily transmitted by the aedes aegypti (l.) mosquito, has rapidly expanded in geographic extent over the past several decades. in some areas, however, dengue fever has not emerged despite established ae. aegypti populations. the reasons for this are unclear and have sometimes been attributed to socio-economic differences. in 2013 we compared ae. aegypti adult density and population age structure between two cities in sonora, mexico: hermosillo, which has regular seasonal dengue ... | 2017 | 28082648 |
| comparison of stable and transient wolbachia infection models in aedes aegypti to block dengue and west nile viruses. | pathogen replication and transmission in wolbachia infected insects are currently studied using three wolbachia infection systems: naturally infected wolbachia hosts, hosts transinfected with wolbachia (stably maintained and inherited infections) and hosts transiently infected with wolbachia. all three systems have been used to test the effect of wolbachia on mosquito transmitted pathogens such as dengue virus (denv), west nile virus (wnv) and plasmodium. from these studies it is becoming increa ... | 2017 | 28052065 |
| optimal control problems of mosquito-borne disease subject to changes in feeding behavior of aedes mosquitoes. | dengue viruses (denv) are transmitted to humans by the bite of aedes mosquitoes. it is known that dengue virus infection in aedes aegypti female mosquitoes makes a change in the feeding behavior of the infected mosquitoes. in this study, using the forces of infection, we incorporated the effect of changes in the feeding behavior of mosquitoes into the standard vector-borne sir-si model. it has been proved that both a single-strain model and a two-strain model exhibit forward bifurcations. moreov ... | 2017 | 28385591 |
| catalase protects aedes aegypti from oxidative stress and increases midgut infection prevalence of dengue but not zika. | digestion of blood in the midgut of aedes aegypti results in the release of pro-oxidant molecules that can be toxic to the mosquito. we hypothesized that after a blood meal, the antioxidant capacity of the midgut is increased to protect cells against oxidative stress. concomitantly, pathogens present in the blood ingested by mosquitoes, such as the arboviruses dengue and zika, also have to overcome the same oxidative challenge, and the antioxidant program induced by the insect is likely to influ ... | 2017 | 28379952 |
| the relationship between entomological indicators of aedes aegypti abundance and dengue virus infection. | routine entomological monitoring data are used to quantify the abundance of ae. aegypti. the public health utility of these indicators is based on the assumption that greater mosquito abundance increases the risk of human denv transmission, and therefore reducing exposure to the vector decreases incidence of infection. entomological survey data from two longitudinal cohort studies in iquitos, peru, linked with 8,153 paired serological samples taken approximately six months apart were analyzed. i ... | 2017 | 28333938 |
| a new paradigm for aedes spp. surveillance using gravid ovipositing sticky trap and ns1 antigen test kit. | dengue remains a serious public health problem in southeast asia and has increased 37-fold in malaysia compared to decades ago. new strategies are urgently needed for early detection and control of dengue epidemics. | 2017 | 28327173 |
| biologic evidence required for zika disease enhancement by dengue antibodies. | the sudden appearance of overt human zika virus infections that cross the placenta to damage fetal tissues, target sexual organs, and are followed in some instances by guillain-barré syndrome raises questions regarding whether these outcomes are caused by genetic mutations or if prior infection by other flaviviruses affects disease outcome. because dengue and zika viruses co-circulate in the urban aedes aegypti mosquito-human cycle, a logical question, as suggested by in vitro data, is whether d ... | 2017 | 28322690 |
| occurrence of concurrent infections with multiple serotypes of dengue viruses during 2013-2015 in northern kerala, india. | dengue is a global human public health threat, causing severe morbidity and mortality. the occurrence of sequential infection by more than one serotype of dengue virus (denv) is a major contributing factor for the induction of dengue hemorrhagic fever (dhf) and dengue shock syndrome (dss), two major medical conditions caused by denv infection. however, there is no specific drug or vaccine available against dengue infection. there are reports indicating the increased incidence of concurrent infec ... | 2017 | 28316881 |
| dengue virus ns2b/ns3 protease inhibitors exploiting the prime side. | the mosquito-transmitted dengue virus (denv) infects millions of people in tropical and sub-tropical regions. maturation of denv particles requires proper cleavage of the viral polyprotein, including processing 8 of the 13 substrate cleavage sites by dengue ns2b-s3 protease. with no available direct-acting antiviral targeting denv, ns2-s3 protease is a promising target for inhibitor design. current design efforts focus on the nonprime side of the denv protease active site, resulting in highly hy ... | 2017 | 28298600 |
| the microbiome composition of aedes aegypti is not critical for wolbachia-mediated inhibition of dengue virus. | dengue virus (denv) is primarily vectored by the mosquito aedes aegypti, and is estimated to cause 390 million human infections annually. a novel method for denv control involves stable transinfection of ae. aegypti with the common insect endosymbiont wolbachia, which mediates an antiviral effect. however, the mechanism by which wolbachia reduces the susceptibility of ae. aegypti to denv is not fully understood. in this study we assessed the potential of resident microbiota, which can play impor ... | 2017 | 28267749 |
| the rnai pathway plays a small part in wolbachia-mediated blocking of dengue virus in mosquito cells. | wolbachia pipientis is an insect endosymbiont known to limit the replication of viruses including dengue and zika in their primary mosquito vector, aedes aegypti. wolbachia is being released into mosquito populations globally in a bid to control the diseases caused by these viruses. it is theorized that wolbachia's priming of the insect immune system may confer protection against subsequent viral infection. other hypotheses posit a role for competition between wolbachia and viruses for host cell ... | 2017 | 28262718 |
| population genomics reveals that an anthropophilic population of aedes aegypti mosquitoes in west africa recently gave rise to american and asian populations of this major disease vector. | the mosquito aedes aegypti is the main vector of dengue, zika, chikungunya and yellow fever viruses. this major disease vector is thought to have arisen when the african subspecies ae. aegypti formosus evolved from being zoophilic and living in forest habitats into a form that specialises on humans and resides near human population centres. the resulting domestic subspecies, ae. aegypti aegypti, is found throughout the tropics and largely blood-feeds on humans. | 2017 | 28241828 |
| [technical guidelines for the prevention and treatment of chikungunya fever]. | chikungunya fever is an emerging disease caused by an alphavirus belonging to the togaviridae family, transmitted by the bite of aedes genus species: aedesaegypti and aedesalbopictus. in 2013, paho/who received confirmation of the first cases of indigenous transmission of chikungunya in the americas. this disease may be acute, subacute and chronic, affecting all age groups. following an incubation period from three to seven days, the patient usually begins with a high fever (greater than 39 °c), ... | 2017 | 25680650 |
| [chikungunya, an emerging viral disease. proposal of an algorithm for its clinical management]. | chikungunya fever (chik) is an emerging viral disease. it is caused by the chikungunya virus, an alphavirus from the togaviridae family. it is transmitted to humans by the bite of infected mosquitoes, mainly aedes aegypti and aedes albopictus. they are also involved in the transmission of dengue, malaria, etc. chik is now endemic in any region of africa and southeast-asia. cases of chik have been reported in america, the caribbean, and europe (france, italy and spain). there are reservoirs of th ... | 2017 | 25440971 |
| entomological investigations during early stages of a chikungunya outbreak in the united states virgin islands, 2014. | during the 2014 chikungunya (chik) outbreak in the caribbean, we performed entomological surveys on 3 united states virgin islands (usvi): st. croix, st. thomas, and st. john. we aimed to evaluate the potential for chikungunya virus (chikv) transmission in the usvi. the surveys took place between june 19, 2014, and june 29, 2014, during the dry season in usvi. a total of 1,929 adult mosquitoes belonging to 4 species- culex quinquefasciatus (68.4%), aedes aegypti (29.7%), ae. mediovittatus (1.3%) ... | 2017 | 28388320 |
| potential of aedes aegypti and aedes albopictus populations in the central african republic to transmit enzootic chikungunya virus strains. | major chikungunya outbreaks have affected several central african countries during the past decade. the chikungunya virus (chikv) was isolated from humans and sylvan mosquitoes in the central african republic (car) during the 1970 and 1980s but has not been found recently, despite the presence of aedes albopictus since 2010. the risk of a massive chikungunya epidemic is therefore potentially high, as the human populations are immunologically naïve and because of the presence of the mosquito vect ... | 2017 | 28347325 |
| computer-aided structure based drug design approaches for the discovery of new anti-chikv agents. | chikungunya is a viral infection caused by chikungunya virus (chikv), an arbovirus transmitted through mosquito (aedes aegypti and aedes albopictus) bite. the virus from sylvatic cycle in africa mutated to new vector adaptation and became one of the major emerging and re-emerging viral infections in the past decade, affecting more than 40 countries. efforts are being made by many researches to develop means to prevent and control the infection through vaccines and vector control strategy. on the ... | 2017 | 28294048 |
| chikungunya infection: a global public health menace. | chikungunya virus (chikv) has been involved in epidemics in african and asian subcontinents and, of late, has transcended to affect the americas. aedes aegypti and aedes albopictus are the major vectors for chikv infection, which results in dissemination of virus to various vital organs. entry of virus into these tissues causes infiltration of innate immune cells, monocytes, macrophages, neutrophils, natural killer cells, and adaptive immune cells. macrophages bearing the replicating virus, in t ... | 2017 | 28233156 |
| aedes-borne virus-mosquito interactions: mass spectrometry strategies and findings. | aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing zika epidemics. aedes mosquitoes spread different arboviruses such as dengue virus (denv), chikungunya virus (chikv), and zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. these viruses have complex transmission cycles that include two hosts, namely the aedes mosquito as a vector and susceptible vertebrate hos ... | 2017 | 28192064 |
| environmental and social change drive the explosive emergence of zika virus in the americas. | since zika virus (zikv) was detected in brazil in 2015, it has spread explosively across the americas and has been linked to increased incidence of microcephaly and guillain-barré syndrome (gbs). in one year, it has infected over 500,000 people (suspected and confirmed cases) in 40 countries and territories in the americas. along with recent epidemics of dengue (denv) and chikungunya virus (chikv), which are also transmitted by aedes aegypti and ae. albopictus mosquitoes, the emergence of zikv s ... | 2017 | 28182667 |
| severe chikungunya infection in northern mozambique: a case report. | although chikungunya virus has rapidly expanded to several countries in sub-saharan africa, little attention has been paid to its control and management. until recently, chikungunya has been regarded as a benign and self-limiting disease. in this report we describe the first case of severe chikungunya disease in an adult patient in pemba, mozambique. | 2017 | 28179029 |
| transcriptome analysis of aedes aegypti in response to mono-infections and co-infections of dengue virus-2 and chikungunya virus. | chikungunya virus (chikv) and dengue virus (denv) spread via the bite of infected aedes mosquitoes. both these viruses exist as co-infections in the host as well as the vector and are known to exploit their cellular machinery for their replication. while there are studies reporting the changes in aedes transcriptome when infected with denv and chikv individually, the effect both these viruses have on the mosquitoes when present as co-infections is not clearly understood. in the present study, we ... | 2017 | 28161634 |
| chikungunya virus: an update on the biology and pathogenesis of this emerging pathogen. | re-emergence of chikungunya virus, a mosquito-transmitted pathogen, is of serious public health concern. in the past 15 years, after decades of infrequent, sporadic outbreaks, the virus has caused major epidemic outbreaks in africa, asia, the indian ocean, and more recently the caribbean and the americas. chikungunya virus is mainly transmitted by aedes aegypti mosquitoes in tropical and subtropical regions, but the potential exists for further spread because of genetic adaptation of the virus t ... | 2017 | 28159534 |
| engineered aedes aegypti jak/stat pathway-mediated immunity to dengue virus. | we have developed genetically modified ae. aegypti mosquitoes that activate the conserved antiviral jak/stat pathway in the fat body tissue, by overexpressing either the receptor dome or the janus kinase hop by the blood feeding-induced vitellogenin (vg) promoter. transgene expression inhibits infection with several dengue virus (denv) serotypes in the midgut as well as systemically and in the salivary glands. the impact of the transgenes dome and hop on mosquito longevity was minimal, but it re ... | 2017 | 28081143 |
| identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, aedes aegypti. | aedes aegypti is a major vector for arboviruses such as dengue, chikungunya and zika viruses. during acquisition of a viremic bloodmeal, an arbovirus infects mosquito midgut cells before disseminating to secondary tissues, including the salivary glands. once virus is released into the salivary ducts it can be transmitted to another vertebrate host. the midgut is surrounded by a basal lamina (bl) in the extracellular matrix, consisting of a proteinaceous mesh composed of collagen iv and laminin. ... | 2017 | 28054419 |
| wingbeat frequency-sweep and visual stimuli for trapping male aedes aegypti (diptera: culicidae). | combinations of female wingbeat acoustic cues and visual cues were evaluated to determine their potential for use in male aedes aegypti (l.) traps in peridomestic environments. a modified centers for disease control (cdc) light trap using a 350-500 hz frequency-sweep broadcast from a speaker as an acoustic stimulus, combined with a black poster-board half-cylinder behind the trap as a visual stimulus, captured a significantly greater proportion of males in a laboratory arena during daylight than ... | 2017 | 28399312 |
| effect of temperature thresholds on modeled aedes aegypti (diptera: culicidae) population dynamics. | dynamic simulation models provide vector abundance estimates using only meteorological data. however, model outcomes may heavily depend on the assumptions used to parameterize them. we conducted a sensitivity analysis for a model of aedes aegypti (l.) abundance using weather data from two locations where this vector is established, la margarita, puerto rico and tucson, arizona. we tested the effect of simplifying temperature-dependent development and mortality rates and of changing development a ... | 2017 | 28399306 |
| factors of concern regarding zika and other aedes aegypti-transmitted viruses in the united states. | the recent explosive outbreaks of zika and chikungunya throughout the americas has raised concerns about the threats that these and similar diseases may pose to the united states (u.s.). the commonly accepted association between tropical climates and the endemicity of these diseases has led to concerns about the possibility of their redistribution due to climate change and transmission arising from cases imported from endemic regions initiating outbreaks in the united states. while such possibil ... | 2017 | 28399294 |
| repellent and larvicidal activity of the essential oil from eucalyptus nitens against aedes aegypti and aedes albopictus (diptera: culicidae). | dengue, chikungunya, and yellow fever are important vector-borne diseases transmitted by female mosquitoes when they feed on humans. the use of repellents based on natural products is an alternative for personal protection against these diseases. application of chemicals with larvicidal activity is another strategy for controlling the mosquito population. the repellent and larvicidal activities of the essential oil from eucalyptus nitens were tested against aedes aegypti and aedes albopictus, th ... | 2017 | 28399283 |
| transcriptional profile for detoxification enzymes aeaggt1 and aaeggt2 from aedes aegypti (diptera: culicidae) in response to larvicides. | aedes aegypti (l.) is the vector responsible for transmitting dengue, chikungunya, yellow fever, and zika viruses, as well as other pathogens. microbial larvicides based on bacillus thuringiensis berliner israelensis (bti) and saccharopolyspora spinosa mertz and yao, such as vectobac 12as and natular 2ec, have been shown to be effective in reducing larval populations of ae. aegypti. we examined the gene expression of two detoxification enzymes, glucosyl and glucuronosyl transferases (aaeggt1 and ... | 2017 | 28399278 |
| detection and establishment of aedes aegypti and aedes albopictus (diptera: culicidae) mosquitoes in california, 2011-2015. | in 2011, a thriving population of aedes albopictus (skuse), the asian tiger mosquito, was discovered within three cities in los angeles county over an estimated 52-km2 urban area. two years later in 2013, aedes aegypti (l.), the yellow fever mosquito, was detected within several urban areas of madera, fresno, and san mateo counties. state and local vector control agencies responded with an aggressive effort to eradicate or interrupt the spread of these two invasive mosquitoes; however, known pop ... | 2017 | 28399270 |
| ecological niche model for predicting distribution of disease-vector mosquitoes in yucatán state, méxico. | the majority of the yucatán state, méxico, presents subtropical climate that is suitable for many species of mosquitoes that are known to be vectors of diseases, including those from the genera aedes and culex. the objective of this study is to identify the geographic distribution of five species from these two genera and estimate the human population at risk of coming in contact with them. we compiled distributional data for aedes aegypti (l.), aedes (howardina) cozumelensis (diaz najera), cule ... | 2017 | 28399263 |
| emergent and reemergent arboviruses in south america and the caribbean: why so many and why now? | several arboviruses have emerged and/or reemerged in the new world in the past decades. zika and chikungunya viruses, formerly restricted to africa and perhaps asia, invaded the continent, causing great concern. dengue virus outbreaks have continued to occur in almost all countries, with millions of cases per year. west nile virus rapidly invaded north america, and now cases have been found in central and south america. other arboviruses, such as mayaro and eastern equine encephalitis viruses, h ... | 2017 | 28399216 |
| eleutherococcus senticosus (araliaceae) leaf morpho-anatomy, essential oil composition, and its biological activity against aedes aegypti (diptera: culicidae). | the roots of eleutherococcus senticosus (rupr. & maxim.) maxim., a well-known medicinal plant from eastern asia, are used worldwide for their known beneficial medicinal properties. recently, the leaves have been used as an alternative to the roots. the present study was aimed at exploring the leaf essential oil as a potential source of compounds for mosquito management. gas chromatography/mass spectrometry analysis of the leaf essential oil revealed 87 compounds, constituting 95.2% of the oil. α ... | 2017 | 28399215 |
| a video-tracking analysis-based behavioral assay for larvae of anopheles pseudopunctipennis and aedes aegypti (diptera: culicidae). | aedes aegypti (l.) is the primary vector of dengue, yellow fever, zika, and chikungunya viruses, whereas anopheles pseudopunctipennis (theobald) is the principal vector for malaria in latin america. the larval stage of these mosquitoes occurs in very different development habitats, and the study of their respective behaviors could give us valuable information to improve larval control. the aim of this study was to set up a bioassay to study basic larval behaviors using a video-tracking software. ... | 2017 | 28399206 |
| differential pathogenicity of metarhizium blastospores and conidia against larvae of three mosquito species. | biorational insecticides are being increasingly used in integrated pest management programs. in laboratory bioassays, the pathogenicity of blastospores and conidia of the entomopathogenic fungus metarhizium brunneum arsef 4556 was evaluated against larvae of three mosquito species. three propagule concentrations (1 × 106, 1 × 107, and 1 × 108 spores ml - 1) were used in the bioassays. results showed that aedes aegypti had lower survival rates when exposed to blastospores than when exposed to con ... | 2017 | 28399202 |
| epidemiological characteristics of dengue disease in latin america and in the caribbean: a systematic review of the literature. | dengue, an important mosquito-borne virus transmitted mainly by aedes aegypti, is a major public health issue in latin america and the caribbean. national epidemiological surveillance systems, usually based on passive detection of symptomatic cases, while underestimating the true burden of dengue disease, can provide valuable insight into disease trends and excess reporting and potential outbreaks. we carried out a systematic review of the literature to characterize the recent epidemiology of de ... | 2017 | 28392806 |
| fabrication of highly effective mosquito nanolarvicides using an asian plant of ethno-pharmacological interest, priyangu (aglaia elaeagnoidea): toxicity on non-target mosquito natural enemies. | mosquitoes threaten the lives of humans, livestock, pets and wildlife around the globe, due to their ability to vector devastating diseases. aglaia elaeagnoidea, commonly known as priyangu, is widely employed in asian traditional medicine and pest control. medicinal activities include anti-inflammatory, analgesic, anticancer, and anesthetic actions. flavaglines, six cyclopenta[b]benzofurans, a cyclopenta[bc]benzopyran, a benzo[b]oxepine, and an aromatic butyrolactone showed antifungal properties ... | 2017 | 28390026 |
| insecticidal and repellent activity of several plant-derived essential oils against aedes aegypti. | we examined the pupicidal, adulticidal, repellent, and oviposition-deterrent activities of essential oils (eos) from lippia alba, l. origanoides, eucalyptus citriodora, cymbopogon citratus, cymbopogon flexuosus, citrus sinensis , cananga odorata , swinglea glutinosa, and tagetes lucida plants against aedes aegypti under laboratory conditions. pupicidal and adulticidal activities were assessed at exploratory concentrations of 250, 310, and 390 parts per million (ppm); and 30, 300, and 1,000 ppm, ... | 2017 | 28388322 |
| in the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito aedes aegypti: an integrated next-generation sequencing approach. | the capacity of aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. metabolic resistance to pyrethroids is common in aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robu ... | 2017 | 28379969 |
| effects of socio-demographic characteristics and household water management on aedes aegypti production in suburban and rural villages in laos and thailand. | dengue fever is a mosquito-borne disease accounting for 50-100 million annual cases globally. laos and thailand are countries in south-east asia where the disease is endemic in both urban and rural areas. household water storage containers, which are favourable breeding sites for dengue mosquitoes, are common in these areas, due to intermittent or limited access to water supply. this study assessed the effect of household water management and socio-demographic risk factors on aedes aegypti infes ... | 2017 | 28376893 |
| new insights into the in vitro development and virulence of culicinomyces spp. as fungal pathogens of aedes aegypti. | culicinomyces spp. (hypocreales: cordycipitaceae) are facultative fungal pathogens affecting the larval stages from a range of mosquito species and are especially notable in their ability to infect hosts through the digestive tract after conidial ingestion. while culicinomyces spp. were studied mainly in the 1980s, little is yet known about inter- and intraspecific variability of the in vitro development of these fungi at different temperatures, and nothing is known about the impact of serial ho ... | 2017 | 28372997 |
| evolutionary origin and function of nox4-art, an arthropod specific nadph oxidase. | nadph oxidases (nox) are ros producing enzymes that perform essential roles in cell physiology, including cell signaling and antimicrobial defense. this gene family is present in most eukaryotes, suggesting a common ancestor. to date, only a limited number of phylogenetic studies of metazoan noxes have been performed, with few arthropod genes. in arthropods, only nox5 and duox genes have been found and a gene called noxm was found in mosquitoes but its origin and function has not been examined. ... | 2017 | 28356077 |
| larvicidal activity of ottonia anisum metabolites against aedes aegypti: a potential natural alternative source for mosquito vector control in brazil. | aedes aegypti mosquito is the principal vector of the viruses responsible for urban yellow fever, dengue, dengue haemorrhagic fever, as well as zika and chikungunya in brazil. the present study was aimed to investigate the insecticidal potential of the extract and fractions of ottonia anisum, along with special metabolites isolated from it, as natural alternatives against larvae (l3) of ae. aegypti, vector of potentially deadly tropical infections in brazil. | 2017 | 28352047 |
| combined target site (kdr) mutations play a primary role in highly pyrethroid resistant phenotypes of aedes aegypti from saudi arabia. | pyrethroid resistance is a threat to effective vector control of aedes aegypti, the vector of dengue, zika and other arboviruses, but there are many major knowledge gaps on the mechanisms of resistance. in jeddah and makkah, the principal dengue-endemic areas of saudi arabia, pyrethroids are used widely for ae. aegypti control but information about resistance remains sparse, and the underlying genetic basis is unknown. findings from an ongoing study in this internationally significant area are r ... | 2017 | 28347352 |
| first report of the phe1534cys kdr mutation in natural populations of aedes albopictus from brazil. | knockdown resistance (kdr), caused by alterations in the voltage-gated sodium channel (nav), is one of the mechanisms responsible for pyrethroid (py) resistance. in the asian tiger mosquito, aedes albopictus, at least four different mutations were described in the iiis6 nav segment in populations from asia, north america and europe. in contrast, in aedes aegypti at least 12 non-synonymous mutations have been reported at nine different codons, mostly in the iis6 and iiis6 nav segments. the phe153 ... | 2017 | 28347326 |
| the toxicity of angiotensin converting enzyme inhibitors to larvae of the disease vectors aedes aegypti and anopheles gambiae. | the control of mosquitoes is threatened by the appearance of insecticide resistance and therefore new control chemicals are urgently required. here we show that inhibitors of mosquito peptidyl dipeptidase, a peptidase related to mammalian angiotensin-converting enzyme (ace), are insecticidal to larvae of the mosquitoes, aedes aegypti and anopheles gambiae. ace inhibitors (captopril, fosinopril and fosinoprilat) and two peptides (trypsin-modulating oostatic factor/tmof and a bradykinin-potentiati ... | 2017 | 28345667 |
| novel synthesis of gold nanoparticles using artemisia vulgaris l. leaf extract and their efficacy of larvicidal activity against dengue fever vector aedes aegypti l. | the aedes aegypti l. mosquito transmits dengue and yellow fever, which cause millions of death every year. dengue is a mosquito-borne viral disease that has rapidly spread worldwide particularly in countries with tropical and subtropical climates areas. the present study denotes a simple and eco-friendly biosynthesis of gold nanoparticles using artemisia vulgaris l. leaf extract as reducing agent. the synthesized gold nanoparticles were characterized by uv-visible spectroscopy, x-ray diffraction ... | 2017 | 28341392 |
| rapid surveillance for vector presence (rsvp): development of a novel system for detecting aedes aegypti and aedes albopictus. | the globally important zika, dengue and chikungunya viruses are primarily transmitted by the invasive mosquitoes, aedes aegypti and aedes albopictus. in australia, there is an increasing risk that these species may invade highly urbanized regions and trigger outbreaks. we describe the development of a rapid surveillance for vector presence (rsvp) system to expedite presence- absence surveys for both species. | 2017 | 28339458 |
| de novo assembly of the aedes aegypti genome using hi-c yields chromosome-length scaffolds. | the zika outbreak, spread by the aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective way. here we combine hi-c data with existing draft assemblies to generate chromosome-length scaffolds. we validate this method by assembling a human genome, de novo, from short reads alone (67× coverage). we then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors aeaegypti and culex quinquefa ... | 2017 | 28336562 |
| functional and nonfunctional forms of cquior91, an odorant selectivity subunit of culex quinquefasciatus. | in culex quinquefasciatus, cquior91 is the ortholog of 2 larvae-specific odorant receptors (ors) from anopheles gambiae (agam\or40, previously shown to respond to several odorant ligands including the broad-spectrum repellent n,n-diethyl-3-methylbenzamide, deet) and aedes aegypti (aaeg\or40). when we cloned full-length cquior91 from a culex quinquefasciatus larval head rna sample, we found 2 alleles of this or, differing at 9 residues. functional analysis using the xenopus oocyte expression syst ... | 2017 | 28334229 |
| screening of wolbachia endosymbiont infection in aedes aegypti mosquitoes using attenuated total reflection mid-infrared spectroscopy. | dengue fever is the most common mosquito transmitted viral infection afflicting humans, estimated to generate around 390 million infections each year in over 100 countries. the introduction of the endosymbiotic bacterium wolbachia into aedes aegypti mosquitoes has the potential to greatly reduce the public health burden of the disease. this approach requires extensive polymerase chain reaction (pcr) testing of the wolbachia-infection status of mosquitoes in areas where wolbachia-a. aegypti are r ... | 2017 | 28332822 |
| retention of duplicated long-wavelength opsins in mosquito lineages by positive selection and differential expression. | opsins are light sensitive receptors associated with visual processes. insects typically possess opsins that are stimulated by ultraviolet, short and long wavelength (lw) radiation. six putative lw-sensitive opsins predicted in the yellow fever mosquito, aedes aegypti and malaria mosquito, anopheles gambiae, and eight in the southern house mosquito, culex quinquefasciatus, suggest gene expansion in the family culicidae (mosquitoes) relative to other insects. here we report the first detailed mol ... | 2017 | 28320313 |
| frequency of aedes sp. linnaeus (diptera: culicidae) and associated entomofauna in bromeliads from a forest patch within a densely urbanized area. | little is known about the relationship between the presence of aedes, abiotic factors and the entomofauna existing in phytotelmatas. the aim of this study was to identify biotic and abiotic factors associated with the presence of aedes in bromeliads sites located in a forest fragment in rio de janeiro, brazil. in the study area, eight bromeliads from the aechmea genus were chosen and kept in landscape form. physical and chemical variables were measured. collected aquatic insects were identified ... | 2017 | 28315189 |
| a dynamic survival detection and analysis system for mosquito larvae viability in drug assays. | bioinformatics studies have emerged in the domain of larval behavior analysis in recent years. a dynamic survival detection and analysis system for automatically monitoring a large amount of mosquito larvae in bioassays with multiwell plates by acquiring and processing videos is proposed in this article. in our system, equipment is designed for acquiring the video of the mosquito larvae in several multiwell plates simultaneously by a camera, and a video analysis module is developed for detecting ... | 2017 | 28314109 |
| toxicity of spinosad to temephos-resistant aedes aegypti populations in brazil. | the mosquito aedes aegypti is the primary vector of different arboviruses and represents a major public health problem. several brazilian populations of ae. aegypti have developed resistance to temephos, the most used organophosphate larvicide. new tools which are less harmful to the environment and safer for humans are becoming increasingly important to control this insect vector. spinosad, an aerobic fermentation product of a soil actinobacteria, has a favorable environmental profile. it prese ... | 2017 | 28301568 |
| evaluation of reference genes at different developmental stages for quantitative real-time pcr in aedes aegypti. | the mosquito aedes aegypti (ae. aegypti) is the most notorious vector of illness-causing viruses such as dengue, chikugunya, and zika. although numerous genetic expression studies utilizing quantitative real-time pcr (qpcr) have been conducted with regards to ae. aegypti, a panel of genes to be used suitably as references for the purpose of expression-level normalization within this epidemiologically important insect is presently lacking. here, the usability of seven widely-utilized reference ge ... | 2017 | 28300076 |
| longitudinal study of thai people media exposure, knowledge, and behavior on dengue fever prevention and control. | dengue hemorrhagic fever is transmitted through a bite by a dengue -infected aedes aegypti mosquito. it was first reported in the mid -20th century in thailand, and since then its epidemiology has been of great concern and has spread all across the country. the alarming incidence of dengue posed a serious threat to human health in all major cities of thailand. this study was aimed at identifying the level of awareness of dengue fever in thai population knowledge for prevention and control, and m ... | 2017 | 28285970 |
| repellency of the origanum onites l. essential oil and constituents to the lone star tick and yellow fever mosquito. | the oregano, origanum onites l., essential oil (eo) was tested in laboratory behavioural bioassays for repellent activity against amblyomma americanum (l.) and aedes aegypti (l.). the o. onites eo was characterised using gc-fid and gc-ms. carvacrol (75.70%), linalool (9.0%), p-cymene (4.33%) and thymol (1.9%) were the most abundant compounds. at a concentration of 0.413 mg oil/cm(2) of filter paper, o. onites eo repelled 100% of the ticks tested and at 0.103 mg oil/cm(2) of filter paper, 66.7% o ... | 2017 | 28278656 |
| mosquito-borne diseases and omics: salivary gland proteome of the female aedes aegypti mosquito. | the female aedes aegypti mosquito is an important vector for several tropical and subtropical diseases such as dengue, chikungunya, and zika and yellow fever. the disease viruses infect the mosquito and subsequently spread to the salivary glands after which the viruses can be transmitted to humans with probing or feeding by the mosquito. omics systems sciences offer the opportunity to characterize vectors and can inform disease surveillance, vector control and development of innovative diagnosti ... | 2017 | 28271980 |
| cre/lox-recombinase-mediated cassette exchange for reversible site-specific genomic targeting of the disease vector, aedes aegypti. | site-specific genome modification (ssm) is an important tool for mosquito functional genomics and comparative gene expression studies, which contribute to a better understanding of mosquito biology and are thus a key to finding new strategies to eliminate vector-borne diseases. moreover, it allows for the creation of advanced transgenic strains for vector control programs. ssm circumvents the drawbacks of transposon-mediated transgenesis, where random transgene integration into the host genome r ... | 2017 | 28266580 |