Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| enterococcus faecalis 6-phosphogluconolactonase is required for both commensal and pathogenic interactions with manduca sexta. | enterococcus faecalis is a commensal and pathogen of humans and insects. in manduca sexta, e. faecalis is an infrequent member of the commensal gut community, but its translocation to the hemocoel results in a commensal-to-pathogen switch. to investigate e. faecalis factors required for commensalism, we identified e. faecalis genes that are upregulated in the gut of m. sexta using recombinase-based in vivo expression technology (rivet). the rivet screen produced 113 clones, from which we identif ... | 2015 | 25385794 |
| the polyamine n-acetyltransferase-like enzyme pmve plays a role in the virulence of enterococcus faecalis. | we previously showed that the mutant strain of enterococcus faecalis lacking the transcriptional regulator slya is more virulent than the parental strain. we hypothesized that this phenotype was due to overexpression of the second gene of the slya operon, ef_3001, renamed pmve (for polyamine metabolism and virulence of e. faecalis). pmve shares strong homologies with n(1)-spermidine/spermine acetyltransferase enzymes involved in the metabolism of polyamines. in this study, we used an e. faecalis ... | 2015 | 25385793 |
| the two-component system grvrs (etars) regulates ace expression in enterococcus faecalis og1rf. | expression of ace (adhesin to collagen of enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions, such as in the presence of serum, bile salts, urine, and collagen and at 46 °c. however, the mechanism of ace/ace regulation under different conditions is still unknown. in this study, we identified a two-component regulatory system grvrs as the main regulator of ace expression under these stress con ... | 2015 | 25385790 |
| an in vivo comparison of internal bacterial colonization in two dental implant systems: identification of a pathogenic reservoir. | the aim of this study was to compare internal bacterial colonization in two implant systems, one screw root form (srf) with an external hexagon connection and one plateau root form (prf) with a morse taper internal connection. | 2015 | 25385682 |
| role of secondary metabolites in establishment of the mutualistic partnership between xenorhabdus nematophila and the entomopathogenic nematode steinernema carpocapsae. | xenorhabdus nematophila engages in a mutualistic partnership with the nematode steinernema carpocapsae, which invades insects, migrates through the gut, and penetrates into the hemocoel (body cavity). we showed previously that during invasion of manduca sexta, the gut microbe staphylococcus saprophyticus appeared transiently in the hemocoel, while enterococcus faecalis proliferated as x. nematophila became dominant. x. nematophila produces diverse secondary metabolites, including the major water ... | 2015 | 25398871 |
| photoactivation of curcumin and sodium hypochlorite to enhance antibiofilm efficacy in root canal dentin. | to test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against enterococcus faecalis biofilms in vitro. | 2015 | 25462576 |
| definitive structural assessment of enterococcal diheteroglycan. | enterococcus faecalis is one of most important nosocomial and often multi-antibiotic resistant pathogens responsible for infections that are difficult to treat. previously, a cell-wall polysaccharide termed diheteroglycan (dhg) was isolated and characterized as a promising vaccine candidate. however, the configuration of its lactic acid (la) residue attached to the galactofuranoside unit was not assessed, although it influences conformation of dhg chain in terms of biological recognition and imm ... | 2015 | 25421964 |
| in vitro and in vivo activity of hyperimmune globulin preparations against multiresistant nosocomial pathogens. | we compared different immunoglobulin preparations containing igg (intraglobin/intratect) or a mixture of igg, iga, and igm (pentaglobin) to assess the opsonic and protective efficacy of human immunoglobulin preparations against multiresistent nosocomial pathogens. | 2015 | 25428225 |
| the question of pathogen quantification in disinfected graywater. | graywater (gw) reuse for irrigation is recognized as a sustainable solution for water conservation. one of the major impediments to gw reuse is the presence of pathogenic microorganisms. this study monitored three similar on-site gw treatment systems bi-monthly over the course of a year to compare the presence of pathogens and indicators in raw, biologically treated, and biologically treated and disinfected [by chlorine and ultraviolet light (uv)] gw. the systems were designed to allow the testi ... | 2015 | 25437766 |
| enterococcus faecalis pcf10-encoded surface proteins prga, prgb (aggregation substance) and prgc contribute to plasmid transfer, biofilm formation and virulence. | enterococcus faecalis pcf10 transfers at high frequencies upon pheromone induction of the prgq transfer operon. this operon codes for three cell wall-anchored proteins - prga, prgb (aggregation substance) and prgc - and a type iv secretion system through which the plasmid is delivered to recipient cells. here, we defined the contributions of the prg surface proteins to plasmid transfer, biofilm formation and virulence using the caenorhabditis elegans infection model. we report that a combination ... | 2015 | 25431047 |
| antimicrobial bioassay-guided fractionation of a methanol extract of eupatorium triplinerve. | eupatorium triplinerve vahl (asteraceae), popularly known as japana, is widely used in folk medicine, due its analgesic, anticoagulant, antianorexic, antiparasitic, anthelmintic, sedative, antifungal, and antibacterial properties. | 2015 | 25430540 |
| atomic force microscopy visualization of injuries in enterococcus faecalis surface caused by er,cr:ysgg and diode lasers. | to visualize by atomic force microscopy the alterations induced on enterococcus. faecalis surface after treatment with 2 types of laser: erbium chromium:yttrium-scandium-gallium-garnet (er,cr:ysgg) laser and diode laser. | 2015 | 25475770 |
| the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening. | the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ... | 2015 | 25475270 |
| transfer of tetracycline resistance genes with aggregation substance in food-borne enterococcus faecalis. | enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (as) and inducible sex pheromones to exchange genetic elements in food matrix. to evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne e. faecalis were collected in korea. among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(m) and tet(l) genes together, followed by tet(m) (19.6 %), and tet(l) (6.8 %) alone. also, we ... | 2015 | 25487115 |
| outcome of aortic valve replacement for active infective endocarditis in patients on chronic hemodialysis. | the high risk of morbidity and mortality for patients on hemodialysis who are undergoing cardiac surgery is increased for those with active infective endocarditis (aie). this retrospective observational single-center study evaluated the impact of chronic hemodialysis on the outcome of aortic valve replacement in patients with aortic aie. | 2015 | 25483000 |
| biosynthesis of silver nanoparticles using ethanolic petals extract of rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities. | the present study was aimed at biosynthesis of silver nanoparticles (agnps) using ethanolic extract of rose (rosa indica) petals and testing their potential antibacterial activity using selective human pathogenic microbes, anticancer activity using human colon adenocarcinoma cancer cell line hct 15 as well as anti-inflammatory activity using rat peritoneal macrophages in vitro. the biologically synthesized agnps were also characterized by uv-visible spectroscopy, scanning electron microscopy (se ... | 2015 | 25481491 |
| the use of the replication region of plasmid prs7 from oenococcus oeni as a putative tool to generate cloning vectors for lactic acid bacteria. | a chimeric plasmid, prs7rep (6.1 kb), was constructed using the replication region of prs7, a large plasmid from oenococcus oeni, and pem64, a plasmid derived from pij2925 and containing a gene for resistance to chloramphenicol. prs7rep is a shuttle vector that replicates in escherichia coli using its pij2925 component and in lactic acid bacteria (lab) using the replication region of prs7. high levels of transformants per µg of dna were obtained by electroporation of prs7rep into pediococcus aci ... | 2015 | 25479060 |
| removal of total and antibiotic resistant bacteria in advanced wastewater treatment by ozonation in combination with different filtering techniques. | elimination of bacteria by ozonation in combination with charcoal or slow sand filtration for advanced sewage treatment to improve the quality of treated sewage and to reduce the potential risk for human health of receiving surface waters was investigated in pilot scale at the sewage treatment plant eriskirch, baden-wuerttemberg/germany. to determine the elimination of sewage bacteria, inflowing and leaving wastewater of different treatment processes was analysed in a culture-based approach for ... | 2015 | 25497174 |
| different vana elements in e. faecalis and in e. faecium suggest at least two origins of tn1546 among vre in a brazilian hospital. | in 2009 during surveillance in a brazilian hospital, many patients were confirmed to be colonized by vancomycin-resistant enterococci (vre) and a few infection cases occurred. among 14 isolates of enterococcus faecalis, most had the same pulsotype, virulence profile (agg(+)elra(+)gele(+)), and were sequence type (st)103, indicating dissemination of a clone. the 47 enterococcus faecium were separated into four pulsotypes, the predominant virulence profile being esp(+)acm(+). all of them harbored ... | 2015 | 25588068 |
| antimicrobial activity of chlorhexidine, peracetic acid and sodium hypochlorite/etidronate irrigant solutions against enterococcus faecalis biofilms. | to evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (naocl) and associated with 9% hebp (naocl/hebp), 2% peracetic acid (paa) and 2% chlorhexidine (chx), on the viability of enterococcus faecalis biofilms attached to dentine. | 2015 | 25515403 |
| photodynamic therapy in root canals contaminated with enterococcus faecalis using curcumin as photosensitizer. | the aim of the study was to evaluate the photodynamic therapy (pdt) effect on root canals contaminated with enterococcus faecalis using a light emitting diode (led) light and a curcumin solution (cur) as photosensitizer (ps). eighty root canals from uniradicular human teeth were prepared with protaper universal rotary system and contaminated with e. faecalis for 21 days. they were divided as: gia-pdt (cur, pre-irradiation for 5 + 5 min of irradiation); gib-pdt (cur, pre-irradiation for 5 + 10 mi ... | 2015 | 25502831 |
| effects of the probiotic enterococcus faecium ncimb 10415 on selected lactic acid bacteria and enterobacteria in co-culture. | enterococcus faecium ncimb 10415 is used as a probiotic for piglets and has been shown to modify the porcine intestinal microbiota. however, the mode of action of this probiotic modification is still unclear. one possible explanation is the direct growth inhibiting or stimulating effect of the probiotic on other indigenous bacteria. therefore, the aim of the present study was to examine the growth interactions of the probiotic with different indigenous porcine bacteria in vitro. reference strain ... | 2015 | 25519527 |
| tyramine biosynthesis is transcriptionally induced at low ph and improves the fitness of enterococcus faecalis in acidic environments. | enterococcus faecalis is a commensal bacterium of the human gut that requires the ability to pass through the stomach and therefore cope with low ph. e. faecalis has also been identified as one of the major tyramine producers in fermented food products, where they also encounter acidic environments. in the present work, we have constructed a non-tyramine-producing mutant to study the role of the tyramine biosynthetic pathway, which converts tyrosine to tyramine via amino acid decarboxylation. wi ... | 2015 | 25529314 |
| using a genome-scale metabolic model of enterococcus faecalis v583 to assess amino acid uptake and its impact on central metabolism. | increasing antibiotic resistance in pathogenic bacteria necessitates the development of new medication strategies. interfering with the metabolic network of the pathogen can provide novel drug targets but simultaneously requires a deeper and more detailed organism-specific understanding of the metabolism, which is often surprisingly sparse. in light of this, we reconstructed a genome-scale metabolic model of the pathogen enterococcus faecalis v583. the manually curated metabolic network comprise ... | 2015 | 25527553 |
| effects of epigallocatechin gallate against enterococcus faecalis biofilm and virulence. | epigallocatechin-3-gallate (egcg), the most abundant polyphenol in green tea (camellia sinesis) has been shown to exert antimicrobial effects on numerous bacterial pathogens. however its efficacy against enterococcus faecalis biofilm, which is associated with persistent root canal infection is unknown. the aims of this study were to investigate the effects of egcg against e. faecalis biofilm and virulence. | 2015 | 25526623 |
| photodynamic killing of enterococcus faecalis in dentinal tubules using mthpc incorporated in liposomes and invasomes. | the present in vitro study investigates the antimicrobial photodynamic efficiency of the photosensitizer 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (mthpc) incorporated in liposomes (lip) and highly flexible invasomes (inv) on the endodontopathogenic species enterococcus faecalis in infected dental root canals. | 2015 | 24950607 |
| influence of working length and apical preparation size on apical bacterial extrusion during reciprocating instrumentation. | to evaluate the influence of reciprocating single-file instrumentation with different working lengths (wl) and apical preparation sizes on apical bacterial extrusion. | 2015 | 25087922 |
| potential for nosocomial transmission of enterococcus faecalis from surfaces in dental operatories. | to assess the potential for nosocomial transmission of enterococcus faecalis during root canal treatment by measuring its occurrence on surfaces in dental operatories in relation to the efficacy of disinfection routines. | 2015 | 25066305 |
| the surface rhamnopolysaccharide epa of enterococcus faecalis is a key determinant of intestinal colonization. | enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients. the pathogenesis of e. faecalis infection relies in part on its capacity to colonize the gut. following disruption of intestinal homeostasis, e. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream. to identify and characterize e. faecalis genes that are key to intestinal colonization, our strategy consisted in scr ... | 2015 | 25035517 |
| autoimmune neutropenia of infancy with recurrent urinary tract infections: a case report. | autoimmune neutropenia of infancy is characterized by minor intercurrent infections despite severe neutropenia; severe bacterial infections are uncommon. an infant developed recurrent urinary tract infections at 9 and 11 months of age. the identified uropathogens were escherichia coli and enterococcus faecalis, respectively. empirical treatment with carbapenems, as broad-spectrum antibiotics, promptly resolved the infection without sequelae. febrile neutropenic children with cancer and autoimmun ... | 2015 | 24942028 |
| characterisation of the antibacterial effect of polyethyleneimine nanoparticles in relation to particle distribution in resin composite. | to characterise the antibacterial effect of resin composite incorporating cross-linked quaternised polyethyleneimine (qpei) nanoparticles in relation to their distribution in the bulk material. | 2015 | 24881908 |
| production of lactic acid using a new homofermentative enterococcus faecalis isolate. | lactic acid is an intermediate-volume specialty chemical for a wide range of food and industrial applications such as pharmaceuticals, cosmetics and chemical syntheses. although lactic acid production has been well documented, improved production parameters that lead to reduced production costs are always of interest in industrial developments. in this study, we describe the production of lactic acid at high concentration, yield and volumetric productivity utilizing a novel homofermentative, fac ... | 2015 | 24894833 |
| modified lactic acid bacteria detect and inhibit multiresistant enterococci. | we designed lactococcus lactis to detect enterococcus faecalis. upon detection, l. lactis produce and secrete antienterococcal peptides. the peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of l. lactis. the enterococcal sex pheromone ccf10 serves as the signal for detection. expression vectors derived from pcf10, a ccf10-responsive e. faecalis sex-pheromone conjugative plasmid, were engineered in l. lactis for the detection system. recombinant host strain ... | 2015 | 24896372 |
| antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or er:yag laser in vitro. | to investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of enterococcus faecalis in vitro. | 2015 | 25080240 |
| commensal bacteria drive endogenous transformation and tumour stem cell marker expression through a bystander effect. | commensal bacteria and innate immunity play a major role in the development of colorectal cancer (crc). we propose that selected commensals polarise colon macrophages to produce endogenous mutagens that initiate chromosomal instability (cin), lead to expression of progenitor and tumour stem cell markers, and drive crc through a bystander effect. | 2015 | 24906974 |
| clonal diversity in biofilm formation by enterococcus faecalis in response to environmental stress associated with endodontic irrigants and medicaments. | to determine whether clonal diversity within e. faecalis affects biofilm formation when exposed to antimicrobial compounds found in endodontic medicaments and irrigants. | 2015 | 24749689 |
| comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white-coloured mineral trioxide aggregate. | to comparatively examine the cell attachment, cytotoxicity, and antibacterial activity of radiopaque dicalcium silicate cement (rdsc) and proroot white-coloured mineral trioxide aggregate (wmta). | 2015 | 24802368 |
| formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (apdt): studies on curcumin and curcuminoids lii. | bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (apdt) are needed. curcumin is under investigation as a potential photosensitizer in apdt. | 2015 | 24842546 |
| assessment of dentinal tubule invasion capacity of enterococcus faecalis under stress conditions ex vivo. | to investigate the dentinal tubule invasion capacity of enterococcus faecalis under alkaline and energy starvation stress conditions. | 2015 | 24872016 |
| effectiveness of ktp laser versus 980 nm diode laser to kill enterococcus faecalis in biofilms developed in experimentally infected root canals. | this study aimed to evaluate the antibacterial action of ktp (potassium-titanyl-phosphate) laser irradiations (compared with 980 nm diode laser), associated with conventional endodontic procedures, on enterococcus faecalis biofilms. fifty-six dental roots with single canals were prepared with ni-ti rotary instruments, autoclaved, inoculated with an e. faecalis suspension and incubated for 72 h. they were randomly allocated to control and treatment groups. laser parameters were as follows: power ... | 2015 | 24588799 |
| diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures? | enterococci represent the most controversial group of dairy bacteria. they are found to be the main constituent of many traditional mediterranean dairy products and contribute to their characteristic taste and flavor. on the other hand, during the last 50 years antibiotic-resistant enterococci have emerged as leading causes of nosocomial infections worldwide. the aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 ente ... | 2015 | 26441888 |
| molecular characterization of microbial contaminants isolated from umbilical cord blood units for transplant. | disposal of umbilical cord blood units due to microbial contamination is a major problem in cord blood banks worldwide as it reduces the number of units available for transplantation. additionally, economic losses are generated as result of resources and infrastructure used to obtain such units. umbilical cord blood units that showed initial microbial contamination were subject to strains isolation, identification, and characterization by sequencing the 16s rrna gene and enterobacterial repetiti ... | 2015 | 26361843 |
| virulence factors genes in enterococci isolated from beavers (castor fiber). | only limited information exists concerning the microbiota in beaver (castor fiber). this study has been focused on the virulence factors genes detection in enterococci from beavers. in general, animals are not affected by enterococcal infections, but they can be a reservoir of, e.g. pathogenic strains. moreover, detection of virulence factors genes in enterococci from beavers was never tested before. free-living beavers (12), male and female (age 4-5 years) were caught in the north-east part of ... | 2015 | 25300356 |
| comparison of apical sealing ability of two phases of gutta-percha: a bacterial leakage model. | the aim of this study was to compare apical sealing ability of alpha and beta phases of gutta-percha by means of bacterial leakage model. | 2015 | 27507995 |
| effect of different obturation materials on residual antimicrobial activity of 2% chlorhexidine in dentin at different time intervals: an ex vivo study. | the aim of this study was to evaluate the effect of gutta percha/ah26 and resilon/realseal se on residual antimicrobial activity of chlorhexidine (chx) in human root dentin and suggest the best filling material when chx is used as final irrigant. | 2015 | 27252755 |
| characterization of enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production. | in this study, 72 enterococcus faecalis isolates originating from humans (n=39), dogs (n=26) and cats (n=7) were investigated for some virulence factors, some virulence genes, antibiotic resistance phenotypes, randomly amplified polymorphic dna-polymerase chain reaction (rapd-pcr) patterns and biofilm production. of the isolates, 31 (43.1%) were positive for gelatinase, 11 (15.3%) for aggregation substance and cytolysine, 38 (52.8%) for gele and 34 (47.2%) for asa1 genes. all isolates were found ... | 2015 | 27175186 |
| enterococcus faecalis biofilm. formation and development in vitro observed by scanning electron microscopy. | biofilm produced by enterococcus faecalis isolated from root canals was detected by growing it on microplates and using 10% crystal violet stain, elution with alcohol and three procedures: no fixation, heat fixation and 10% formaldehyde fixation. the biofilm was evaluated using a versamax microplate reader (usa). twenty sterile root portions were incubated in ts broth with e. faecalis (108) for 48 hours, 4, 7, 14 and 30 days, after which they were processed and observed by scanning electron micr ... | 2015 | 27095620 |
| antimicrobial activity of herbal medicines (tulsi extract, neem extract) and chlorhexidine against enterococcus faecalis in endodontics: an in vitro study. | successful endodontic treatment depends on effective disinfection and complete sealing of root canal. various medicaments are advised for disinfecting root canal, such as herbal and non-herbal medicaments. this study was done to assess the antimicrobial activity of herbal medicines (neem extract, tulsi extract) and chlorhexidine against enterococcus faecalis in endodontics. | 2015 | 26942123 |
| [clinical observations of acute-onset endophthalmitis after clear corneal phacoemulsification]. | to evaluate the incidence of acute-onset endophthalmitis after clear corneal phacoemulsification and to report its clinical features, microbiology, and therapeutic efficacy. | 2015 | 26888274 |
| cholesterol suppresses antimicrobial effect of statins. | isoprenoid biosynthesis is a key metabolic pathway to produce a wide variety of biomolecules such as cholesterol and carotenoids, which target cell membranes. on the other hand, it has been reported that statins known as inhibitors of isoprenoid biosynthesis and cholesterol lowering agents, may have a direct antimicrobial effect on the some bacteria. the exact action of statins in microbial metabolism is not clearly understood. it is possible that statins inhibit synthesis or utilization of some ... | 2015 | 26877857 |
| did the iceman know better? screening of the medicinal properties of the birch polypore medicinal mushroom, piptoporus betulinus (higher basidiomycetes). | the birch polypore piptoporus betulinus was among two mushrooms that were found in the iceman's bag. recent studies indicated that p. betulinus was probably used as a religious and medicinal item. in order to examine the medicinal potential of p. betulinus, hot water (hw), partially purified (pp), and alkali extract (ha) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin i-converting enzyme (ace) inhibitory activity. all tested samples exhibited moderate cytotoxi ... | 2015 | 26854098 |
| [the incidence and risk factors of male urinary tract infection following invasive urodynamic investigation]. | to assess the prevalence of male urinary tract infection (uti) after invasive urodynamic studies and to determinate the risk factors by evaluating the clinical characteristics and urodynamic parameters of patients. | 2015 | 26813139 |
| enterococcus faecalis from food, clinical specimens, and oral sites: prevalence of virulence factors in association with biofilm formation. | enterococci have gained significance as the cause of nosocomial infections; they occur as food contaminants and have also been linked to dental diseases. e. faecalis has a great potential to spread virulence as well as antibiotic resistance genes via horizontal gene transfer. the integration of food-borne enterococci into the oral biofilm in-vivo has been observed. therefore, we investigated the virulence determinants and antibiotic resistance of 97 e. faecalis isolates from the oral cavity, foo ... | 2015 | 26793174 |
| global regulation of gene expression by the mafr protein of enterococcus faecalis. | enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. however, as an opportunistic pathogen, it is able to colonize other host niches and cause life-threatening infections. its adaptation to new environments involves global changes in gene expression. the ef3013 gene (here named mafr) of e. faecalis strain v583 encodes a protein (mafr, 482 residues) that has sequence similarity to global response regulators of the mga/atxa family. the enterococcal og1rf genome also e ... | 2015 | 26793169 |
| enterococcus faecalis as multidrug resistance strains in clinical isolates in imam reza hospital in kermanshah, iran. | the current study aimed to investigate the prevalence of vancomycin-resistant enterococcus in e. faecalis and e. faecium and antimicrobial susceptibility patterns, then dominant genes responsible for vancomycin resistance were determined. for this propose, 180 clinical isolates of enterococcus were subjected for identification and antibiotic susceptibility assay. then, the gene responsible vancomycin resistant strains were determined. the results demonstrated the e. faecalis as a dominant entero ... | 2015 | 26738400 |
| the influence of urinary tract infections on treatment of fracture of proximal femur in patients with systemiv osteoporosis. | in the structure of intrahospital infections the infections of urinary tract make up from 20 to 40%. this problem represents special medical-social significance for trauma departments as patients with trauma are being in forced situation for along time. 380 patients older than 60 years (average age 73.6 ± 9.2 years) with a fracture of proximal femur against background of the systemic osteoporosis having in-patient treatment in the trauma department of the republic hospital no2 were being examine ... | 2015 | 26887137 |
| [a case of pyoderma gangrenosum of the penis difficult to distinguish from fournier gangrene]. | here, we report a case of pyoderma gangrenosum of the penis which was difficult to distinguish from fournier gangrene. the patient was a 54-year-old male who was aware of redness and swelling of the glanspenis for 1 month prior to a consultation at our department. although he was diagnosed with herpes and treated at a nearby hospital, his symptoms did not improve. subsequently, the patient visited our department following the onset of pain and fever. during his initial consultation, he had a fev ... | 2015 | 26699892 |
| evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of enterococcus faecalis. | background and aims. triple antibiotic paste (tap) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. the aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of tap at 1-, 2-, 3-, and 4-week intervals on mature enterococcus faecalis biofilm. materials and methods. a total of 287 extracted one-rooted human central incisors were infected with e. faecalis atcc 29212 after removing the crown and preparation ... | 2015 | 26697145 |
| the capability of tyramine production and correlation between phenotypic and genetic characteristics of enterococcus faecium and enterococcus faecalis strains. | the aim of this study was to investigate the diversity of tyramine production capability of four enterococcus strains in buffered systems in relation to their genetic characteristics and environmental conditions. cells of the strains enterococcus faecalis ef37 and atcc 29212, and e. faecium fc12 and fc643 were re-suspended in phosphate/citrate buffers with different ph, nacl concentration and incubation temperature. at intervals, cell viability and tyramine production were assessed by plate coun ... | 2015 | 26696981 |
| olive mill waste extracts: polyphenols content, antioxidant, and antimicrobial activities. | natural polyphenols extracts have been usually associated with great bioactive properties. in this work, we investigated in vitro antioxidant and antimicrobial potential of the phenolic olive mill wastewater extracts (owwe) and the olive cake extracts (oce). using the folin ciocalteux method, owwe contained higher total phenol content compared to oce (8.90 ± 0.728 g/l versus 0.95 ± 0.017 mg/g). the phenolic compounds identification was carried out with a performance liquid chromatograph coupled ... | 2015 | 26693221 |
| enterococcus faecalis septicemia and vertebral osteomyelitis after transrectal ultrasound guided biopsy of the prostate. | transrectal ultrasound guided prostate biopsy (trus) has rarely been associated with disseminated infection, yet the occurrence appears to be increasing. resistance to fluoroquinolones, the most commonly used prophylaxis, is one of the likely causes, with escherichia coli being the most commonly reported cause of these infections. herein we present what is, to our knowledge, the first case of enterococcus faecalis septicemia and vertebral osteomyelitis after trus. previously reported cases of th ... | 2015 | 26682075 |
| genomic sequence of a clinical vancomycin-resistant reference strain, enterococcus faecalis atcc 51299. | in this paper, we present a draft genome sequence of a quality control reference strain, enterococcus faecalis atcc 51299 (multilocus sequencing type [mlst] st6), which is sensitive to teicoplanin but resistant to vancomycin. it is used in an agar screening test for streptomycin, gentamicin, and vancomycin resistance and the resistance marker vanb. | 2015 | 26679597 |
| an in vitro evaluation of antimicrobial activity of five herbal extracts and comparison of their activity with 2.5% sodium hypochlorite against enterococcus faecalis. | sodium hypochlorite is the most widely used irrigant in endodontic practice, but it has various disadvantages. literature has shown that herbal products such as propolis, azadirachta indica (ai), triphala, curcuma longa, and morinda citrifolia (mc) possess good antimicrobial properties and thus can be used as potential endodontic irrigants. | 2015 | 26672425 |
| anti-microbial efficacy of allium sativum extract against enterococcus faecalis biofilm and its penetration into the root dentin: an in vitro study. | sodium hypochlorite (naocl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effective led to this study. | 2015 | 26672416 |
| the effect of addition of an eps degrading enzyme with and without detergent to 2% chlorhexidine on disruption of enterococcus faecalis biofilm: a confocal laser scanning microscopic study. | enterococcus faecalis is one of the most commonly occurring organisms retrieved from root canal treated teeth that show refractory apical periodontitis. though it is well known that the ability of e. faecalis to form a matrix-encased biofilm contributes to its pathogenicity, the role of extracellular dextran and dna in biofilm formation and its effect on the susceptibility of the biofilm to chlorhexidine remains poorly understood. it was hypothesized that the addition of an extracellular polymer ... | 2015 | 26675655 |
| hydrothermally treated chitosan hydrogel loaded with copper and zinc particles as a potential micronutrient-based antimicrobial feed additive. | large-scale use of antibiotics in food animal farms as growth promoters is considered as one of the driving factors behind increasing incidence of microbial resistance. several alternatives are under investigation to reduce the amount of total antibiotics used in order to avoid any potential transmission of drug resistant microbes to humans through food chain. copper sulfate and zinc oxide salts are used as feed supplement as they exhibit antimicrobial properties in addition to being micronutrie ... | 2015 | 26664989 |
| degree of bacterial microleakage at the implant-abutment junction in cone morse tapered implants under loaded and unloaded conditions. | different results have been reported on the internal colonization of cone morse connections under in vitro dynamic loading. the aim of the present in vitro study was to evaluate the bacterial leakage in cone morse implant-abutment connections, both under loaded and unloaded conditions. | 2015 | 26660625 |
| tricuspid valve endocarditis complicated by mobitz type ii heart block - a case report and literature review. | we present a case of a middle-aged male who manifested with low-grade fever and lower back pain. mri and bone scan of the spine were suggestive of vertebral osteomyelitis. blood cultures were persistently positive for enterococcus faecalis and echocardiogram revealed tricuspid valve endocarditis. there was no history of iv drug use and urine toxicology was negative. ekg showed mobitz type ii av block and a transesophageal echocardiogram revealed no valve ring or septal abscesses. the heart block ... | 2015 | 26653699 |
| antibacterial and toxic effect of hydrogen peroxide combined with different concentrations of chlorhexidine in comparison with sodium hypochlorite. | hydrogen peroxide (h2o2) has been suggested to be used in sequence or in combination with chlorhexidine (chx) to enhance the antibacterial activity against enterococcus faecalis, but there is no research in the literature on the safety and effectiveness of this irrigation protocol. | 2015 | 26636124 |
| in vitro comparison of antimicrobial effectiveness of qmix and other final irrigants in human root canals. | final root canal irrigation stands as an effective strategy for eliminating the dentin infection. this study aimed to investigate and compare the antibacterial efficacy of qmix and other four final irrigation regimens in reducing enterococcus faecalis within human root canals. single-canal human teeth contaminated with e. faecalis for 4 weeks were prepared chemomechanically with sodium hypochlorite (naocl). then, the teeth were randomly assigned into six groups according to the final irrigation ... | 2015 | 26631907 |
| clinical and microbiological features of bacteremia caused by enterococcus faecalis. | enterococcus faecalis is a frequent etiologic agent of invasive infections in hospitalized patients. the aim of this study was to analyze clinical and microbiological features of bacteremia caused by e. faecalis. | 2015 | 26623628 |
| synergistic antibacterial effect of the combination of ε-polylysine and nisin against enterococcus faecalis. | this study evaluated the antibacterial effect of the combination of ε-polylysine (ε-pl) and nisin against enterococcus faecalis strains. the combination of ε-pl and nisin showed synergistic antibacterial activity against three enterococcus strains. scanning electron microscopy and a membrane permeability assay revealed that the combined treatment with ε-pl and nisin synergistically damaged the cell morphology of e. faecalis strain r612z1 cells. both ε-pl and nisin can dissipate the transmembrane ... | 2015 | 26613915 |
| infective endocarditis involving the pulmonary valve. | pulmonary valve (pv) infective endocarditis (ie) is a rare entity, accounting for 1.5% to 2% of cases of ie. published data are limited to a few case series and reports. we sought to review the mayo clinic experience and describe clinical, echocardiographic, and microbiologic features. we included all patients aged ≥18 years seen from 2000 to 2014 who had a diagnosis of native pv ie and unequivocal echocardiographic involvement of the pv. nine patients with pv ie were identified. isolated pv ie ... | 2015 | 26611123 |
| biological changes of enterococcus faecalis in the viable but nonculturable state. | enterococcus faecalis may enter a viable but nonculturable (vbnc) state under adverse conditions. e. faecalis, the major bacterial species present in failed root canal treatments, is thought to survive after endodontic treatment by entering a vbnc state. in this study, we characterized the vbnc state of e. faecalis. we designed 3 different protocols to successfully induce the vbnc state. approximately one-third of bacteria entered a vbnc state after 15-30 days, and all remained viable for at lea ... | 2015 | 26600540 |
| antibiotic resistance and biofilm formation of enterococcus faecalis in patient and environmental samples. | enterococci are opportunistic pathogens and are a major factor in nosocomial infections. they may contain ebp operon, which upon expression makes them highly prone to biofilm formation on biotic and abiotic surfaces. | 2015 | 26587208 |
| assessment of the potential of cfc (calcium hydroxide flagyl ciprofloxacin) for the rapid disinfection of resilon and gutta-percha. | obturating materials exposed to the dental operating environment has been shown to be contaminated, making rapid chair side disinfection mandatory to ensure the sterility of the root canals. | 2015 | 26557614 |
| actions of probiotics on trinitrobenzenesulfonic acid-induced colitis in rats. | we investigated the actions of probiotics, streptococcus faecalis 129 bio 3b (sf3b), in a trinitrobenzenesulfonic acid- (tnbs-) induced colitis model in rats. after tnbs was administered into the colons of rats for induction of colitis, the rats were divided into two groups: one group was given a control diet and the other group was given a diet containing sf3b for 14 days. there were no apparent differences in body weight, diarrhea period, macroscopic colitis score, and colonic weight/length ra ... | 2015 | 26550572 |
| in vitro activity of oritavancin and comparator agents against staphylococci, streptococci and enterococci from clinical infections in europe and north america, 2011-2014. | oritavancin is a lipoglycopeptide that has been approved for the treatment of acute bacterial skin and skin-structure infections (absssis) caused by susceptible organisms. oritavancin causes cell death by inhibiting cell wall synthesis as well as depolarising and permeabilising the cellular membrane of gram-positive pathogens. the activities of oritavancin in comparison with vancomycin, daptomycin and linezolid were determined against a collection of over 11000 recent clinical gram-positive isol ... | 2015 | 26541881 |
| comparative evaluation of effect of different irrigation solutions against enterococcus faecalis: a polymerase chain reaction-based study. | enterococcus faecalis is the most isolated or detected species from oral infections including marginal periodontitis, infected root canals, periradicular abscesses and also detected in cases of failed endodontic therapy. to prevent endodontic treatment failure irrigation is mandatory for the effective removal of smear layer, pulp tissue, and microorganisms. cultivation and other traditional identification methods have been demonstrated to have several limitations when it comes to microbiological ... | 2015 | 26538921 |
| microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation. | this study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (cmp). | 2015 | 26521147 |
| influence of a subinhibitory concentration of vancomycin on the in vitro expression of virulence-related genes in the vancomycin-resistant enterococcus faecalis. | exposure to subinhibitory concentrations (sics) of antimicrobials may alter the bacterial transcriptome. | 2015 | 26516976 |
| rapidly progressive gas-containing lumbar spinal epidural abscess. | gas-containing (emphysematous) infections of the abdomen, pelvis, and extremities are well-known disease entities, which can potentially be life-threatening. they require aggressive medical and often surgical treatment. in the neurosurgical field, some cases of gas-containing brain abscess and subdural empyema have been reported. sometimes they progress rapidly and even can cause fatal outcome. however, gas-containing spinal epidural abscess has been rarely reported and clinical course is unknow ... | 2015 | 26512268 |
| design and activity of a cyclic mini-β-defensin analog: a novel antimicrobial tool. | we have designed a cyclic 17-amino acid β-defensin analog featuring a single disulfide bond. this analog, designated "amc" (ie, antimicrobial cyclic peptide), combines the internal hydrophobic domain of hbd1 and the c-terminal charged region of hbd3. the novel peptide was synthesized and characterized by nuclear magnetic resonance spectroscopy. the antimicrobial activities against gram-positive and gram-negative bacteria as well as against herpes simplex virus type 1 were analyzed. the cytotoxic ... | 2015 | 26508857 |
| structural analysis of streptococcus pyogenes nadh oxidase: conformational dynamics involved in formation of the c(4a)-peroxyflavin intermediate. | in probing the oxygen reactivity of an enterococcus faecalis nadh oxidase (nox; o2 → 2h2o) c42s mutant lacking the cys42-sulfenic acid (cys42-soh) redox center, we provided direct evidence of a c(4a)-peroxyflavin intermediate in the oxidative half-reaction and also described a conformational or chemical change that is rate-limiting for full reoxidation of the homodimer. in this work, the nox from streptococcus pyogenes (spynox) has been expressed and crystallized, and the overoxidized wild-type ... | 2015 | 26506002 |
| functional analysis of genes involved in the biosynthesis of enterocin nkr-5-3b, a novel circular bacteriocin. | a putative biosynthetic gene cluster of the enterocin nkr-5-3b (ent53b), a novel circular bacteriocin, was analyzed by sequencing the flanking regions around enkb, the ent53b structural gene, using a fosmid library. a region approximately 9 kb in length was obtained, and the enkb1, enkb2, enkb3, and enkb4 genes, encoding putative biosynthetic proteins involved in the production, maturation, and secretion of ent53b, were identified. we also determined the identity of proteins mediating self-immun ... | 2015 | 26503847 |
| activity of fosfomycin and comparison of several susceptibility testing methods against contemporary urine isolates. | fosfomycin is recommended as first-line treatment for acute uncomplicated cystitis in women. it has demonstrated in vitro activity against a variety of pathogens; however, a paucity of data are available from the usa. we determined the susceptibility of a collection of urine isolates to fosfomycin and compared multiple methods of susceptibility testing. consecutive non-duplicate enterobacteriaceae, enterococci and pseudomonas aeruginosa isolates were collected from the clinical microbiology labo ... | 2015 | 26498988 |
| selective digestive decontamination and enterococcus faecalis overgrowth and infection: a robust relation is yet unproven. | 2015 | 26495899 | |
| virus satellites drive viral evolution and ecology. | virus satellites are widespread subcellular entities, present both in eukaryotic and in prokaryotic cells. their modus vivendi involves parasitism of the life cycle of their inducing helper viruses, which assures their transmission to a new host. however, the evolutionary and ecological implications of satellites on helper viruses remain unclear. here, using staphylococcal pathogenicity islands (sapis) as a model of virus satellites, we experimentally show that helper viruses rapidly evolve resi ... | 2015 | 26495848 |
| antibacterial efficacy of polymer containing nanoparticles in comparison with sodium hypochlorite in infected root canals. | the aim of the present study was to compare the antibacterial properties of plga polymer containing zno, ag and zno/ag nanoparticles with those of 2.5% naocl in root canals contaminated with enterococcus faecalis. | 2015 | 26486202 |
| antimicrobial activity of silver doped fabrics for the production of hospital uniforms. | among several alternatives to control hospital-acquired infections (hais), a strategy could be the use of hospital uniforms imbued with antimicrobial substances. for this purpose we evaluated the antibacterial activity of two different silver doped fabrics employed for the production of hospital uniforms. the study was conducted in two-step. in the first the antimicrobial activity was evaluated in vitro against escherichia coli atcc 25922, pseudomonas aeruginosa atcc 27853, staphylococcus aureus ... | 2015 | 26485013 |
| bacteriocin production augments niche competition by enterococci in the mammalian gastrointestinal tract. | enterococcus faecalis is both a common commensal of the human gastrointestinal tract and a leading cause of hospital-acquired infections. systemic infections with multidrug-resistant enterococci occur subsequent to gastrointestinal colonization. preventing colonization by multidrug-resistant e. faecalis could therefore be a valuable approach towards limiting infection. however, little is known about the mechanisms e. faecalis uses to colonize and compete for stable gastrointestinal niches. phero ... | 2015 | 26479034 |
| a comparison of apical bacterial extrusion in manual, protaper rotary, and one shape rotary instrumentation techniques. | apical extrusion of irrigants and debris is an inherent limitation associated with cleaning and shaping of root canals and has been studied extensively because of its clinical relevance as a cause of flare-ups. many factors affect the amount of extruded intracanal materials. the purpose of this study was to assess the bacterial extrusion by using manual, multiple-file continuous rotary system (protaper) and single-file continuous rotary system (one shape). | 2015 | 26472680 |
| clinical characteristics and risk factors of enterococcal infections in nagasaki, japan: a retrospective study. | enterococcus spp. are particularly important etiological agents of nosocomial infections. however, the clinical characteristics of and risk factors for enterococcal infections in clinical settings are poorly understood. | 2015 | 26471715 |
| preliminary evaluation of the research-use-only (ruo) icubate ic-gpc assay for identification of select gram-positive bacteria and their resistance determinants in blood culture broths. | the ic-gpc assay (icubate, huntsville, al) provides a molecular option for the rapid, on-demand analysis of positive blood cultures. a preliminary evaluation of the ic-gpc assay using 203 clinical or seeded specimens demonstrated a sensitivity of 93.8% to 100% and a specificity of 98.0% to 100% for the identification of five gram-positive bacterial species (staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, enterococcus faecalis, and enterococcus faecium) and three asso ... | 2015 | 26468498 |
| modeling and application of a rapid fluorescence-based assay for biotoxicity in anaerobic digestion. | the sensitivity of anaerobic digestion metabolism to a wide range of solutes makes it important to be able to monitor toxicants in the feed to anaerobic digesters to optimize their operation. in this study, a rapid fluorescence measurement technique based on resazurin reduction using a microplate reader was developed and applied for the detection of toxicants and/or inhibitors to digesters. a kinetic model was developed to describe the process of resazurin reduced to resorufin, and eventually to ... | 2015 | 26457928 |
| tumor necrosis factor receptor-associated factor 6 plays a role in the inflammatory responses of human periodontal ligament fibroblasts to enterococcus faecalis. | enterococcus faecalis is a frequently isolated microorganism in persistent periapical lesion or secondary infection. however, no evidence has demonstrated that e. faecalis induced inflammation directly in the apical area. this study aimed to explore the mechanism of the inflammatory responses of human periodontal ligament fibroblasts (pdls) to e. faecalis. | 2015 | 26454720 |
| characterization of mobile staphylococcus equorum plasmids isolated from fermented seafood that confer lincomycin resistance. | the complete nucleotide sequences of lincomycin-resistance gene (lnua)-containing plasmids in staphylococcus equorum strains isolated from the high-salt-fermented seafood jeotgal were determined. these plasmids, designated pselnu1-3, are 2638-bp long, have two polymorphic sites, and encode typical elements found in plasmids that replicate via a rolling-circle mechanism including the replication protein gene (rep), a double-stranded origin of replication, a single-stranded origin of replication, ... | 2015 | 26448648 |
| labdane and clerodane diterpenoids from colophospermum mopane. | five labdane (1-5), an isolabdane (6), and five clerodane diterpenoids (7-11), were isolated from seeds, husks, and leaves of colophospermum mopane. compounds 1-3 and 6-9 are new, and their structures were elucidated by means of physical data analysis (1d and 2d nmr, hresims). the absolute configurations of 1, 7, and 10 were determined by single-crystal x-ray diffraction with cu kα radiation. for compounds 2 and 6, the absolute configurations were established by the modified mosher's method and ... | 2015 | 26444248 |
| faster and economical screening for vancomycin-resistant enterococci by sequential use of chromogenic agar and real-time polymerase chain reaction. | screening for vancomycin-resistant enterococci (vre) by culture takes days to generate results, while polymerase chain reaction (pcr) testing directly from clinical specimens lacks specificity. the aims of this study were to develop a real-time pcr to detect and identify enterococcus faecium, enterococcus faecalis, and vana and vanb genes, and to evaluate the impact of this pcr on test-reporting times when performing it directly from suspect vre isolates present on screening chromogenic media. | 2015 | 26442675 |
| clinico-microbiological investigation of catheter associated urinary tract infection by enterococcus faecalis: vana genotype. | prolonged hospitalization and exposure to third generation cephalosporins are reported to facilitate the acquisition and colonization of vancomycin resistant enterococci (vre). though vre is not uncommon in india, urinary tract infection with a vana genotype is a cause of serious concern as vre co-exhibit resistance to aminoglycosides. in india, majority of the vre isolates recovered from hospitalized patients include enterococcus faecium. we report a case of catheter associated urinary tract in ... | 2015 | 26435949 |