Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| apoptosis, autophagy and unfolded protein response pathways in arbovirus replication and pathogenesis. | arboviruses are pathogens that widely affect the health of people in different communities around the world. recently, a few successful approaches toward production of effective vaccines against some of these pathogens have been developed, but treatment and prevention of the resulting diseases remain a major health and research concern. the arbovirus infection and replication processes are complex, and many factors are involved in their regulation. apoptosis, autophagy and the unfolded protein r ... | 2016 | 26781343 |
| viral metagenomics on blood-feeding arthropods as a tool for human disease surveillance. | surveillance and monitoring of viral pathogens circulating in humans and wildlife, together with the identification of emerging infectious diseases (eids), are critical for the prediction of future disease outbreaks and epidemics at an early stage. it is advisable to sample a broad range of vertebrates and invertebrates at different temporospatial levels on a regular basis to detect possible candidate viruses at their natural source. however, virus surveillance systems can be expensive, costly i ... | 2016 | 27775568 |
| an updated checklist of mosquito species (diptera: culicidae) from madagascar. | an updated checklist of 235 mosquito species from madagascar is presented. the number of species has increased considerably compared to previous checklists, particularly the last published in 2003 (178 species). this annotated checklist provides concise information on endemism, taxonomic position, developmental stages, larval habitats, distribution, behavior, and vector-borne diseases potentially transmitted. the 235 species belong to 14 genera: aedeomyia (3 species), aedes (35 species), anophel ... | 2016 | 27101839 |
| the insect-specific palm creek virus modulates west nile virus infection in and transmission by australian mosquitoes. | insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. these viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. following the isolation of the first australian insect-specific flavivirus (isf), palm creek virus (pcv), we investigated routes of infection and transmission of this virus in key australian arbovirus vectors and its impact on replication ... | 2016 | 27457250 |
| development and validation of a quantitative, one-step, multiplex, real-time reverse transcriptase pcr assay for detection of dengue and chikungunya viruses. | dengue virus (denv) and chikungunya virus (chikv) are important human pathogens with common transmission vectors and similar clinical presentations. patient care may be impacted by the misdiagnosis of denv and chikv in areas where both viruses cocirculate. in this study, we have developed and validated a one-step multiplex reverse transcriptase pcr (rt-pcr) to simultaneously detect, quantify, and differentiate between four denv serotypes (pan-denv) and chikungunya virus. the assay uses taqman te ... | 2016 | 27098955 |
| vector competence of aedes aegypti and aedes polynesiensis populations from french polynesia for chikungunya virus. | from october 2014 to march 2015, french polynesia experienced for the first time a chikungunya outbreak. two aedes mosquitoes may have contributed to chikungunya virus (chikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito. | 2016 | 27144888 |
| vertical transmission of indian ocean lineage of chikungunya virus in aedes aegypti and aedes albopictus mosquitoes. | the re-emergence of chikungunya (chik) fever in thailand has been caused by a novel lineage of chikungunya virus (chikv) termed the indian ocean lineage (iol). the aedes albopictus mosquito is thought to be a primary vector of chik fever in thailand, whereas ae. aegypti acts as a secondary vector of the virus. the vertical transmission is believed to be a primary means to maintain chikv in nature and may be associated with an increased risk of outbreak. therefore, the goal of this study was to a ... | 2016 | 27108077 |
| membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying aedes-dengue virus interaction. | understanding the interaction between aedes vectors and dengue viruses (denv) has significant implications in determining the transmission dynamics of dengue. the absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured denv. data obtained from such studies may not reflect the natural human-mosquito transmission scenario. this study explored the potential of using me ... | 2016 | 27083158 |
| rapid detection strategies for the global threat of zika virus: current state, new hypotheses, and limitations. | the current scenario regarding the widespread zika virus (zikv) has resulted in numerous diagnostic studies, specifically in south america and in locations where there is frequent entry of travelers returning from zikv-affected areas, including pregnant women with or without clinical symptoms of zikv infection. the world health organization, who, announced that millions of cases of zikv are likely to occur in the usa in the near future. this situation has created an alarming public health emerge ... | 2016 | 27822207 |
| characterization of a novel murine model to study zika virus. | the mosquito-borne zika virus (zikv) is responsible for an explosive ongoing outbreak of febrile illness across the americas. zikv was previously thought to cause only a mild, flu-like illness, but during the current outbreak, an association with guillain-barré syndrome and microcephaly in neonates has been detected. a previous study showed that zikv requires murine adaptation to generate reproducible murine disease. in our study, a low-passage cambodian isolate caused disease and mortality in m ... | 2016 | 27022155 |
| epidemiology of emergent madariaga encephalitis in a region with endemic venezuelan equine encephalitis: initial host studies and human cross-sectional study in darien, panama. | neurotropic arboviral infections are an important cause of encephalitis. a zoonotic, vector-borne alphavirus, madariaga virus (madv; formerly known as south american eastern equine encephalitis virus), caused its first documented human outbreak in 2010 in darien, panama, where the genetically similar venezuelan equine encephalitis virus (veev) is endemic. we report the results of a seroprevalence survey of animals and humans, illustrating contrasting features of madv and veev ecology and epidemi ... | 2016 | 27101567 |
| the role of the membrane in the structure and biophysical robustness of the dengue virion envelope. | the dengue virion is surrounded by an envelope of membrane proteins surrounding a lipid bilayer. we have combined the cryoelectron microscopy structures of the membrane proteins (pdb: 3j27) with a lipid bilayer whose composition is based on lipidomics data for insect cell membranes, to obtain a near-atomic resolution computational model of the envelope of the dengue virion. a coarse-grained molecular dynamics simulation on the microsecond timescale enables analysis of key biophysical properties ... | 2016 | 26833387 |
| intra-epidemic evolutionary dynamics of a dengue virus type 1 population reveal mutant spectra that correlate with disease transmission. | dengue virus (denv) is currently the most prevalent mosquito-borne viral pathogen. denvs naturally exist as highly heterogeneous populations. even though the descriptions on denv diversity are plentiful, only a few studies have narrated the dynamics of intra-epidemic virus diversity at a fine scale. such accounts are important to decipher the reciprocal relationship between viral evolutionary dynamics and disease transmission that shape dengue epidemiology. in the current study, we present a mic ... | 2016 | 26940650 |
| zika virus and its implication in transfusion safety. | 2016 | 26969782 | |
| transcriptome analysis of the midgut of the chinese oak silkworm antheraea pernyi infected with antheraea pernyi nucleopolyhedrovirus. | the antheraea pernyi nucleopolyhedrovirus (apnpv) is an exclusive pathogen of a. pernyi. the intense interactions between apnpv and a. pernyi cause a series of physiological and pathological changes to a. pernyi. however, no detailed report exists regarding the molecular mechanisms underlying the interactions between apnpv and a. pernyi. in this study, four cdna libraries of the a. pernyi midgut, including two apnpv-infected groups and two control groups, were constructed for transcriptomic anal ... | 2016 | 27820844 |
| prolonged shedding of chikungunya virus in semen and urine: a new perspective for diagnosis and implications for transmission. | we report the presence of chikungunya (chikv) rna in the blood, urine and semen during the acute phase of the disease in an adult with a dual infection with dengue virus type 3. the patient, a 25 yr-old man from salvador, brazil, reported a 6-day duration of high fever, arthralgia, myalgia, headache and photophobia.blood and semen specimens were positive for chikv in the first collected samples; semen and urine specimens were positive for chikv after 30 days of symptoms onset. denv-3 rna was pos ... | 2016 | 27882301 |
| genome sequence of dengue virus 3 from the pythium insidiosum transcriptomes. | 2016 | 27379056 | |
| zika virus: new clinical syndromes and its emergence in the western hemisphere. | zika virus (zikv) had remained a relatively obscure flavivirus until a recent series of outbreaks accompanied by unexpectedly severe clinical complications brought this virus into the spotlight as causing an infection of global public health concern. in this review, we discuss the history and epidemiology of zikv infection, recent outbreaks in oceania and the emergence of zikv in the western hemisphere, newly ascribed complications of zikv infection, including guillain-barré syndrome and microce ... | 2016 | 26962217 |
| the global spread of zika virus: is public and media concern justified in regions currently unaffected? | zika virus, an aedes mosquito-borne flavivirus, is fast becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly among newborn infants in brazil. | 2016 | 27093860 |
| zika virus: the latest newcomer. | since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: west nile, influenza a, avian flu, dengue, chikungunya, sars, mers, ebola, and now zika, the latest newcomer. zika virus (zikv), a flavivirus transmitted by aedes mosquitoes, was identified in 1947 in a sentinel monkey in uganda, and later on in humans in nigeria. the virus was mainly confined to the african continent until it was detected in south-east asia the 1980's, t ... | 2016 | 27148186 |
| clinical and epidemiological characterization of laboratory-confirmed autochthonous cases of zika virus disease in mexico. | since 2014, autochthonous circulation of zika virus (zikv) in the americas was detected (easter island, chile). in may 2015, brazil confirmed autochthonous --transmission and in october of that year colombia reported their first cases. now more than 52 countries have reported cases, including mexico. to deal with this contingency in mexico, several surveillance systems, in addition to systems for vector-borne diseases were strengthened with the participation of all health institutions. also, th ... | 2016 | 27158557 |
| computational approach towards exploring potential anti-chikungunya activity of selected flavonoids. | chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya infection in humans. despite the widespread distribution of chikv, no antiviral medication or vaccine is available against this virus. therefore, it is crucial to find an effective compound to combat chikv. we aimed to predict the possible interactions between non-structural protein 3 (nsp) of chikv as one of the most important viral elements in chikv intracellular replication and 3 potential flavonoids using a comp ... | 2016 | 27071308 |
| zika virus infections imported from brazil to portugal, 2015. | zika virus is an emerging arbovirus transmitted by aedes sp. mosquitoes like the dengue and chikungunya viruses. zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. in 2007, an epidemic in micronesia initiated the turnover in the epidemiological history of zika virus and more recently, the potential association with congenital microcephaly cases in brazil 2015, still under investigation, led the world health organiz ... | 2016 | 27134823 |
| estimating risks of importation and local transmission of zika virus infection. | background. an international spread of zika virus (zikv) infection has attracted global attention. zikv is conveyed by a mosquito vector, aedes species, which also acts as the vector species of dengue and chikungunya viruses. methods. arrival time of zikv importation (i.e., the time at which the first imported case was diagnosed) in each imported country was collected from publicly available data sources. employing a survival analysis model in which the hazard is an inverse function of the effec ... | 2016 | 27069825 |
| identify-isolate-inform: a tool for initial detection and management of zika virus patients in the emergency department. | first isolated in 1947 from a monkey in the zika forest in uganda, and from mosquitoes in the same forest the following year, zika virus has gained international attention due to concerns for infection in pregnant women potentially causing fetal microcephaly. more than one million people have been infected since the appearance of the virus in brazil in 2015. approximately 80% of infected patients are asymptomatic. an association with microcephaly and other birth defects as well as guillain-barre ... | 2016 | 27330653 |
| recombinant dengue 2 virus ns3 helicase protein enhances antibody and t-cell response of purified inactivated vaccine. | dengue virus purified inactivated vaccines (piv) are highly immunogenic and protective over the short term, but may be poor at inducing cell-mediated immune responses and long-term protection. the dengue nonstructural protein 3 (ns3) is considered the main target for t-cell responses during viral infection. the amino (n)-terminal protease and the carboxy (c)-terminal helicase domains of denv-2 ns3 were expressed in e. coli and analyzed for their immune-potentiating capacity. mice were immunized ... | 2016 | 27035715 |
| zika virus: diagnostics for an emerging pandemic threat. | zika virus (zikv) is anaedesmosquito-borne flavivirus that emerged in brazil in 2015 and then rapidly spread throughout the tropical and subtropical americas. based on clinical criteria alone, zikv cannot be reliably distinguished from infections with other pathogens that cause an undifferentiated systemic febrile illness, including infections with two common arboviruses, dengue virus and chikungunya virus. this minireview details the methods that are available to diagnose zikv infection. | 2016 | 26888897 |
| maternal antibody-mediated disease enhancement in type i interferon-deficient mice leads to lethal disease associated with liver damage. | epidemiological studies have reported that most of the severe dengue cases occur upon a secondary heterologous infection. furthermore, babies born to dengue immune mothers are at greater risk of developing severe disease upon primary infection with a heterologous or homologous dengue virus (denv) serotype when maternal antibodies reach sub-neutralizing concentrations. these observations have been explained by the antibody mediated disease enhancement (ade) phenomenon whereby heterologous antibod ... | 2016 | 27007501 |
| nucleotide composition of the zika virus rna genome and its codon usage. | rna viruses have genomes with a distinct nucleotide composition and codon usage. we present the global characteristics of the rna genome of zika virus (zikv), an emerging pathogen within the flavivirus genus. zikv was first isolated in 1947 in uganda, caused a widespread epidemic in south and central america and the caribbean in 2015 and has recently been associated with microcephaly in newborns. | 2016 | 27278486 |
| pathogenesis of zika virus-associated embryopathy. | a strong causal association has become evident between zika virus (zikv) infection during pregnancy and the occurrence of fetal growth restriction, microcephaly and eye defects. circumstantial evidence is presented in this paper in support of the hypothesis that these effects, as well as the guillain-barré syndrome, are due to an endogenous form of hypervitaminosis a resulting from zikv infection-induced damage to the liver and the spillage of stored vitamin a compounds ("retinoids") into the ma ... | 2016 | 27403405 |
| long-term safety and immunogenicity of a tetravalent live-attenuated dengue vaccine and evaluation of a booster dose administered to healthy thai children. | we evaluated the safety and immunogenicity of two doses of a live-attenuated, tetravalent dengue virus vaccine (f17/pre formulation) and a booster dose in a dengue endemic setting in two studies. seven children (7- to 8-year-olds) were followed for 1 year after dose 2 and then given a booster dose (f17/pre formulation), and followed for four more years (child study). in the infant study, 49 2-year-olds, vaccinated as infants, were followed for approximately 3.5 years after dose 2 and then given ... | 2016 | 27022153 |
| scoping review of the zika virus literature. | the global primary literature on zika virus (zikv) (n = 233 studies and reports, up to march 1, 2016) has been compiled using a scoping review methodology to systematically identify and characterise the literature underpinning this broad topic using methods that are documented, updateable and reproducible. our results indicate that more than half the primary literature on zikv has been published since 2011. the articles mainly covered three topic categories: epidemiology of zikv (surveillance an ... | 2016 | 27244249 |
| quantifying the epidemiological impact of vector control on dengue. | 2016 | 27227829 | |
| a pan-dengue virus reverse transcription-insulated isothermal pcr assay intended for point-of-need diagnosis of dengue virus infection by use of the pockit nucleic acid analyzer. | dengue virus (denv) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal pcr (rt-iipcr) method for the detection of all four serotypes of denv in clinical samples. the diagnostic performance of the newly established pan-denv rt-iipc ... | 2016 | 27030492 |
| determinants of arbovirus vertical transmission in mosquitoes. | vertical transmission (vt) and horizontal transmission (ht) of pathogens refer to parental and non-parental chains of host-to-host transmission. combining ht with vt enlarges considerably the range of ecological conditions in which a pathogen can persist, but the factors governing the relative frequency of each transmission mode are poorly understood for pathogens with mixed-mode transmission. elucidating these factors is particularly important for understanding the epidemiology of arthropod-bor ... | 2016 | 27171170 |
| physicians, primary caregivers and topical repellent: all under-utilised resources in stopping dengue virus transmission in affected households. | primary health care facilities frequently manage dengue cases on an ambulatory basis for the duration of the patient's illness. there is a great opportunity for specific messaging, aimed to reduce dengue virus (denv) transmission in and around the home, to be directly targeted toward this high-risk ambulatory patient group, as part of an integrated approach to dengue management. the extent however, to which physicians understand, and can themselves effectively communicate strategies to stop foca ... | 2016 | 27164168 |
| coeloconic sensilla on the antennae of the yellow fever mosquito, aedes aegypti (l.). | 2016 | 5026452 | |
| introduction and transmission of zika virus in brazil: new challenges for the americas. | 2016 | 27007567 | |
| comparative genomic analysis of pre-epidemic and epidemic zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. | less than 20 sporadic cases of human zika virus (zikv) infection were reported in africa and asia before 2007, but large outbreaks involving up to 73% of the populations on the pacific islands have started since 2007, and spread to the americas in 2014. moreover, the clinical manifestation of zikv infection has apparently changed, as evident by increasing reports of neurological complications, such as guillain-barré syndrome in adults and congenital anomalies in neonates. we comprehensively comp ... | 2016 | 26980239 |
| outbreak of zika virus disease in the americas and the association with microcephaly, congenital malformations and guillain-barré syndrome. | 2016 | 26998633 | |
| human antibody response to dengue virus: implications for dengue vaccine design. | dengue, a global health threat, is a leading cause of morbidity and mortality in most tropical and subtropical countries. dengue can range from asymptomatic, relatively mild dengue fever to severe and life-threatening dengue hemorrhagic fever. disease severity and outcome is largely associated with the host immune response. several candidate vaccines in clinical trials appear promising as effective measures for dengue disease control. vaccine development has been hampered by safety and efficacy ... | 2016 | 27398060 |
| zika virus and pregnancy: a review of the literature and clinical considerations. | the latest zika virus (zikv) outbreak has reached epidemic proportions as it spreads throughout south and central america. in november 2015, the brazilian ministry of health reported a 20-fold increase in the number of cases of neonatal microcephaly, which corresponds geographically and temporally to the zikv outbreak. case reports have provided some evidence of a causal link between maternal zikv infection, fetal microcephaly, and intracranial calcifications. the sparse data regarding zikv in p ... | 2016 | 26939047 |
| dna microarray platform for detection and surveillance of viruses transmitted by small mammals and arthropods. | viruses transmitted by small mammals and arthropods serve as global threats to humans. most emergent and re-emergent viral agents are transmitted by these groups; therefore, the development of high-throughput screening methods for the detection and surveillance of such viruses is of great interest. in this study, we describe a dna microarray platform that can be used for screening all viruses transmitted by small mammals and arthropods (smaviruschip) with nucleotide sequences that have been depo ... | 2016 | 27654806 |
| detection of invasive mosquito vectors using environmental dna (edna) from water samples. | repeated introductions and spread of invasive mosquito species (ims) have been recorded on a large scale these last decades worldwide. in this context, members of the mosquito genus aedes can present serious risks to public health as they have or may develop vector competence for various viral diseases. while the tiger mosquito (aedes albopictus) is a well-known vector for e.g. dengue and chikungunya viruses, the asian bush mosquito (ae. j. japonicus) and ae. koreicus have shown vector competenc ... | 2016 | 27626642 |
| new paradigms for virus detection, surveillance and control of zika virus vectors in the settings of southeast asia. | zika virus (zikv) has now become a global public health concern. the vectors for zikv are aedes aegypti and a. albopictus. both these mosquitoes are predominant in southeast asia and are also responsible for the spread of other arboviral diseases like dengue virus and chikungunya virus. the incidence of dengue has been increasing over the years and this is of concern to public health workers. simple laboratory tools for the detection of zikv is also lacking. in the absence of drugs and vaccine f ... | 2016 | 27679623 |
| nonstructural proteins are preferential positive selection targets in zika virus and related flaviviruses. | the flavivirus genus comprises several human pathogens such as dengue virus (denv), japanese encephalitis virus (jev), and zika virus (zikv). although zikv usually causes mild symptoms, growing evidence is linking it to congenital birth defects and to increased risk of guillain-barré syndrome. zikv encodes a polyprotein that is processed to produce three structural and seven nonstructural (ns) proteins. we investigated the evolution of the viral polyprotein in zikv and in related flaviviruses (d ... | 2016 | 27588756 |
| molecular signatures associated with zikv exposure in human cortical neural progenitors. | zika virus (zikv) infection causes microcephaly and has been linked to other brain abnormalities. how zikv impairs brain development and function is unclear. here we systematically profiled transcriptomes of human neural progenitor cells exposed to asian zikv(c), african zikv(m), and dengue virus (denv). in contrast to the robust global transcriptome changes induced by denv, zikv has a more selective and larger impact on expression of genes involved in dna replication and repair. while overall e ... | 2016 | 27580721 |
| zika fetal neuropathogenesis: etiology of a viral syndrome. | the ongoing zika virus epidemic in the americas and the observed association with both fetal abnormalities (primary microcephaly) and adult autoimmune pathology (guillain-barré syndrome) has brought attention to this neglected pathogen. while initial case studies generated significant interest in the zika virus outbreak, larger prospective epidemiology and basic virology studies examining the mechanisms of zika viral infection and associated pathophysiology are only now starting to be published. ... | 2016 | 27560129 |
| modeling mosquito-borne disease spread in u.s. urbanized areas: the case of dengue in miami. | expansion of mosquito-borne pathogens into more temperate regions of the world necessitates tools such as mathematical models for understanding the factors that contribute to the introduction and emergence of a disease in populations naïve to the disease. often, these models are not developed and analyzed until after a pathogen is detected in a population. in this study, we develop a spatially explicit stochastic model parameterized with publicly available u.s. census data for studying the poten ... | 2016 | 27532496 |
| a community-based prospective cohort study of dengue viral infection in malaysia: the study protocol. | globally, dengue infections constitute a significant public health burden. in recent decades, malaysia has become a dengue hyper-endemic country with the co-circulation of the four dengue virus serotypes. the cyclical dominance of sub-types contributes to a pattern of major outbreaks. the consequences can be observed in the rising incidence of reported dengue cases and dengue related deaths. understanding the complex interaction of the dengue virus, its human hosts and the mosquito vectors at th ... | 2016 | 27510731 |
| development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease. | dengue disease is one of the most significant vector-borne illnesses in the world. the emergence and re-emergence of dengue infections in many parts of the world affect millions annually and continue to burden public health systems especially in low-income populations. advances in dengue vaccine development showed promising results; however, protection seems to be suboptimal. there is no licensed chemotherapeutic agent against dengue to date. an ideal scenario of combinatorial vaccination of hig ... | 2016 | 27551237 |
| larval application of sodium channel homologous dsrna restores pyrethroid insecticide susceptibility in a resistant adult mosquito population. | mosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses and other parasitic microorganisms. the emergence and spread of insecticide resistance is threatening the effectiveness of current control measures for common mosquito vector borne diseases, such as malaria, dengue and zika. therefore, the emerging resistance to the widely used pyrethroid insecticides is an alarming problem for public health. herein we demonstrated the use of rna interference ... | 2016 | 27416771 |
| animals in the zika virus life cycle: what to expect from megadiverse latin american countries. | zika virus (zikv) was first isolated in 1947 in primates in uganda, west africa. the virus remained confined to the equatorial regions of africa and asia, cycling between infecting monkeys, arboreal mosquitoes, and occasionally humans. the zikv asiatic strain was probably introduced into brazil in or around late 2013. presently, zikv is in contact with the rich biodiversity in all brazilian biomes, bordering on other latin american countries. infections in brazilian primates have been reported r ... | 2016 | 28005902 |
| artificial diets for mosquitoes. | mosquito-borne diseases are responsible for more than a million human deaths every year. modern mosquito control strategies such as sterile insect technique (sit), release of insects carrying a dominant lethal (ridl), population replacement strategies (pr), and wolbachia-based strategies require the rearing of large numbers of mosquitoes in culture for continuous release over an extended period of time. anautogenous mosquitoes require essential nutrients for egg production, which they obtain thr ... | 2016 | 28009851 |
| diversity of cultivable midgut microbiota at different stages of the asian tiger mosquito, aedes albopictus from tezpur, india. | aedes aegypti and ae. albopictus are among the most important vectors of arboviral diseases, worldwide. recent studies indicate that diverse midgut microbiota of mosquitoes significantly affect development, digestion, metabolism, and immunity of their hosts. midgut microbiota has also been suggested to modulate the competency of mosquitoes to transmit arboviruses, malaria parasites etc. interestingly, the midgut microbial flora is dynamic and the diversity changes with the development of vectors ... | 2016 | 27941985 |
| flaviviruses as a cause of undifferentiated fever in sindh province, pakistan: a preliminary report. | arboviral diseases are expanding worldwide, yet global surveillance is often limited due to diplomatic and cultural barriers between nations. with human encroachment into new habitats, mosquito-borne viruses are also invading new areas. the actual prevalence of expanding arboviruses is unknown in pakistan due to inappropriate diagnosis and poor testing for arboviral diseases. the primary objective of this study was to document evidence of flavivirus infections as the cause of undifferentiated fe ... | 2016 | 26909342 |
| changes in the proteome of langat-infected ixodes scapularis ise6 cells: metabolic pathways associated with flavivirus infection. | ticks (family ixodidae) transmit a variety of disease causing agents to humans and animals. the tick-borne flaviviruses (tbfs; family flaviviridae) are a complex of viruses, many of which cause encephalitis and hemorrhagic fever, and represent global threats to human health and biosecurity. pathogenesis has been well studied in human and animal disease models. equivalent analyses of tick-flavivirus interactions are limited and represent an area of study that could reveal novel approaches for tbf ... | 2016 | 26859745 |
| the asian tiger hunts in maputo city--the first confirmed report of aedes (stegomyia) albopictus (skuse, 1895) in mozambique. | increasing evidence suggests that dengue fever is endemic in mozambique. larvae of both the afrotropical vector aedes aegypti and its subspecies, ae. aegypti formosus, have been reported from three provinces in mozambique, two of which recently experienced dengue outbreaks. despite reports of the invasive oriental vector ae. albopictus on the islands in the mozambique channel and nearby indian ocean, the species has not yet been reported in mozambique. | 2016 | 26856329 |
| cross-protection induced by japanese encephalitis vaccines against different genotypes of dengue viruses in mice. | dengue viruses (denvs) and japanese encephalitis virus (jev) are closely related mosquito-borne flaviviruses that cause very high global disease burdens. although cross-reactivity and cross-protection within flaviviruses have been demonstrated, the effect of jev vaccination on susceptibility to denv infection has not been well elucidated. in this study, we found that vaccination with the jev inactivated vaccine (inv) and live attenuated vaccine (lav) could induce cross-immune responses and cross ... | 2016 | 26818736 |
| long-lasting immune protection and other epidemiological findings after chikungunya emergence in a cambodian rural community, april 2012. | the east/central/south african genotype of chikungunya virus with the e1-a226v mutation emerged in 2011 in cambodia and spread in 2012. an outbreak of 190 cases was documented in trapeang roka, a rural village. we surveyed 425 village residents within 3-4 weeks after the outbreak, and determined the sensitivity and specificity of case definitions and factors associated with infection by chikv. self-reported clinical presentation consisted mostly of fever, rash and arthralgia. the presence of all ... | 2016 | 26752630 |
| behavioral response of aedes aegypti mosquito towards essential oils using olfactometer. | aedes aegypti mosquito is responsible for transmitting human diseases like dengue and chikungunya. personal or space protection with insect repellents is a practical approach to reducing human mosquito contact, thereby minimizing disease transmission. essential oils are natural volatile substances from plants used as protective measure against blood-sucking mosquitoes. | 2016 | 27308295 |
| screening of methanolic plant extracts against larvae of aedes aegypti and anopheles stephensi in mysore. | mosquitoes transmit serious human diseases, causing millions of death every year. vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. nine different locally available medicinally important plants suspected to posse larvicidal property were screened against fourth instar larvae of aedes aegypti and anopheles stephensi to a series of concentrations of ... | 2016 | 27308289 |
| insecticide resistance and management strategies in urban ecosystems. | the increased urbanization of a growing global population makes imperative the development of sustainable integrated pest management (ipm) strategies for urban pest control. this emphasizes pests that are closely associated with the health and wellbeing of humans and domesticated animals. concurrently there are regulatory requirements enforced to minimize inadvertent exposures to insecticides in the urban environment. development of insecticide resistance management (irm) strategies in urban eco ... | 2016 | 26751480 |
| analysis of genotype diversity and evolution of dengue virus serotype 2 using complete genomes. | dengue is one of the most common arboviral diseases prevalent worldwide and is caused by dengue viruses (genus flavivirus, family flaviviridae). there are four serotypes of dengue virus (denv-1 to denv-4), each of which is further subdivided into distinct genotypes. denv-2 is frequently associated with severe dengue infections and epidemics. denv-2 consists of six genotypes such as asian/american, asian i, asian ii, cosmopolitan, american and sylvatic. comparative genomic study was carried out t ... | 2016 | 27635316 |
| detection of chikungunya virus circulation using sugar-baited traps during a major outbreak in french guiana. | 2016 | 27606960 | |
| sequence diversity of dengue virus type 2 in brain and thymus of infected interferon receptor ko mice: implications for dengue virulence. | we previously reported that a clinical isolate of dengue virus (denv) is capable of causing acute-phase systemic infection in mice harboring knockouts of the genes encoding type-i and -ii interferon ifn receptors (ifn-α/β/γr ko mice); in contrast, other virulent denv isolates exhibited slow disease progression in this mice, yielding lethal infection around 20 days post-infection (p.i.). in the present study, we sought to clarify the dynamics of slow disease progression by examining disease progr ... | 2016 | 27903277 |
| febrile illness diagnostics and the malaria-industrial complex: a socio-environmental perspective. | global prioritization of single-disease eradication programs over improvements to basic diagnostic capacity in the global south have left the world unprepared for epidemics of chikungunya, ebola, zika, and whatever lies on the horizon. the medical establishment is slowly realizing that in many parts of sub-saharan africa (ssa), particularly urban areas, up to a third of patients suffering from acute fever do not receive a correct diagnosis of their infection. | 2016 | 27855644 |
| persistence of neutralizing antibody against dengue virus 2 after 70 years from infection in nagasaki. | this study aimed to investigate the duration of humoral immune responses to dengue virus (denv) infection in japanese who experienced acute febrile illness with hemorrhagic manifestations 70 years ago, when an epidemic of dengue occurred in nagasaki, japan, from 1942 to 1944. a japanese volunteer requested serological diagnosis of denv infection in 2014 and donated blood sample to measure the antibody titer against denv by antiflavi igg indirect elisa, focus reduction neutralization test, and pl ... | 2016 | 27493841 |
| assessment of angelica sinensis (oliv.) diels as a repellent for personal protection against mosquitoes under laboratory and field conditions in northern thailand. | angelica sinensis (oliv.) hexane extract (ahe) has been reported as a proven and impressive repellent against laboratory-reared female aedes aegypti mosquitoes. with the aim of promoting products of plant origin as a viable alternative to conventional synthetic substances, this study was designed to transform ahe-based repellents for exploitable commercial production by enhancing their efficacy and assessing their physical and biological stability as well as repellency against mosquitoes under l ... | 2016 | 27357395 |
| dengue virus genome uncoating requires ubiquitination. | the process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, zika, and west nile viruses. currently, dengue virus is one of the most significant human viral pathogens transmitted by mosquitoes and is responsible for about 390 million infections every year around the world. here, we examined for the first time molecular aspect ... | 2016 | 27353759 |
| flavivirus ns1 protein in infected host sera enhances viral acquisition by mosquitoes. | the arbovirus life cycle involves viral transfer between a vertebrate host and an arthropod vector, and acquisition of virus from an infected mammalian host by a vector is an essential step in this process. here, we report that flavivirus nonstructural protein-1 (ns1), which is abundantly secreted into the serum of an infected host, plays a critical role in flavivirus acquisition by mosquitoes. the presence of dengue virus (denv) and japanese encephalitis virus ns1s in the blood of infected inte ... | 2016 | 27562253 |
| prevention and control of zika as a mosquito-borne and sexually transmitted disease: a mathematical modeling analysis. | the ongoing zika virus (zikv) epidemic in the americas poses a major global public health emergency. while zikv is transmitted from human to human by bites of aedes mosquitoes, recent evidence indicates that zikv can also be transmitted via sexual contact with cases of sexually transmitted zikv reported in argentina, canada, chile, france, italy, new zealand, peru, portugal, and the usa. yet, the role of sexual transmission on the spread and control of zikv infection is not well-understood. we i ... | 2016 | 27312324 |
| role of aedes aegypti (linnaeus) and aedes albopictus (skuse) in local dengue epidemics in taiwan. | aedes mosquitoes in taiwan mainly comprise aedes albopictus and ae. aegypti. however, the species contributing to autochthonous dengue spread and the extent at which it occurs remain unclear. thus, in this study, we spatially analyzed real data to determine spatial features related to local dengue incidence and mosquito density, particularly that of ae. albopictus and ae. aegypti. | 2016 | 27829399 |
| low sensitivity of the tourniquet test for differential diagnosis of dengue: an analysis of 28,000 trials in patients. | the aim of this study was to evaluate the utility of the tourniquet test (tt) for dengue diagnosing. to our knowledge, no previous study with such a large sample, of this duration, with as many laboratory methods referenced, or relating the results of the tt to the 2009 who classification of severity has been conducted thus far. | 2016 | 27809813 |
| vertical transmission of zika virus in aedes aegypti mosquitoes. | previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. in this study, designed to test whether zika virus (zikv) could be vertically transmitted, female aedes aegypti and aedes albopictus were injected with zikv, and their f1 adult progeny were tested for zikv ... | 2016 | 27573623 |
| unsuspected dengue as a cause of acute febrile illness in children and adults in western nicaragua. | dengue is an emerging infectious disease of global significance. suspected dengue, especially in children in nicaragua's heavily-urbanized capital of managua, has been well documented, but unsuspected dengue among children and adults with undifferentitated fever has not. | 2016 | 27792777 |
| vector competence of american mosquitoes for three strains of zika virus. | in 2015, zika virus (zikv; flaviviridae; flavivirus) emerged in the americas, causing millions of infections in dozens of countries. the rapid spread of the virus and the association with disease outcomes such as guillain-barré syndrome and microcephaly make understanding transmission dynamics essential. currently, there are no reports of vector competence (vc) of american mosquitoes for zikv isolates from the americas. further, it is not clear whether zikv strains from other genetic lineages ca ... | 2016 | 27783679 |
| a review of the mosquito species (diptera: culicidae) of bangladesh. | diseases caused by mosquito-borne pathogens remain an important source of morbidity and mortality in bangladesh. to better control the vectors that transmit the agents of disease, and hence the diseases they cause, and to appreciate the diversity of the family culicidae, it is important to have an up-to-date list of the species present in the country. original records were collected from a literature review to compile a list of the species recorded in bangladesh. | 2016 | 27770829 |
| zika virus (zikv). | 2016 | 27994533 | |
| looking for inhibitors of the dengue virus ns5 rna-dependent rna-polymerase using a molecular docking approach. | the dengue virus (denv) nonstructural protein 5 (ns5) contains both an n-terminal methyltransferase domain and a c-terminal rna-dependent rna polymerase domain. polymerase activity is responsible for viral rna synthesis by a de novo initiation mechanism and represents an attractive target for antiviral therapy. the incidence of denv has grown rapidly and it is now estimated that half of the human population is at risk of becoming infected with this virus. despite this, there are no effective dru ... | 2016 | 27784988 |
| zika virus on a spreading spree: what we now know that was unknown in the 1950's. | zika virus (zikv) is a mosquito-borne flavivirus that is transmitted through the bite of aedes spp mosquitoes and less predominantly, through sexual intercourse. prior to 2007, zikv was associated with only sporadic human infections with minimal or no clinical manifestations. recently the virus has caused disease outbreaks from the pacific islands, the americas, and off the coast of west africa with approximately 1.62 million people suspected to be infected in more than 60 countries around the g ... | 2016 | 27716242 |
| full genome sequence and sfrna interferon antagonist activity of zika virus from recife, brazil. | the outbreak of zika virus (zikv) in the americas has transformed a previously obscure mosquito-transmitted arbovirus of the flaviviridae family into a major public health concern. little is currently known about the evolution and biology of zikv and the factors that contribute to the associated pathogenesis. determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative proces ... | 2016 | 27706161 |
| management of infection by the zika virus. | a panel of national experts was convened by the brazilian infectious diseases society in order to organize the national recommendations for the management of zika virus infection. the focus of this document is the diagnosis, both clinical and laboratorial, and appropriate treatment of the diverse manifestations of this infection, ranging from acute mild disease to guillain-barré syndrome and also microcephaly and congenital malformations. | 2016 | 27686610 |
| the golgi associated eri3 is a flavivirus host factor. | dengue virus (denv) is a mosquito-borne flavivirus classified into four serotypes (denv-1-4) that causes dengue fever (df), dengue hemorrhagic fever (dhf) or dengue shock syndrome (dss). an estimated 390 million people are at risk for infection with denv and there are no effective vaccines or therapeutics. we utilized rna chromatography coupled with quantitative mass spectrometry (qms) to identify host rna binding proteins (rbps) that interact with denv-2 rna. we identified eri3 (also prnpip and ... | 2016 | 27682269 |
| discovery of novel viruses in mosquitoes from the zambezi valley of mozambique. | mosquitoes carry a wide variety of viruses that can cause vector-borne infectious diseases and affect both human and veterinary public health. although mozambique can be considered a hot spot for emerging infectious diseases due to factors such as a rich vector population and a close vector/human/wildlife interface, the viral flora in mosquitoes have not previously been investigated. in this study, viral metagenomics was employed to analyze the viral communities in culex and mansonia mosquitoes ... | 2016 | 27682810 |
| synthesis and insecticidal activity of acridone derivatives to aedes aegypti and culex quinquefasciatus larvae and non-target aquatic species. | a serious mosquito borne yellow fever is one of the grave diseases which affect the major population. since there is no specific treatment for yellow fever, there is a necessity to develop an effective agent. the series of acridinone analogues 3 to 5 were synthesized with help of non-conventional microwave heating and confirmed by respective spectral characterization. 5c and 3b showed highest activity to kill 90% of larvae against a. aegypti and c. quinquefasciatus, respectively. also the active ... | 2017 | 28059104 |
| discovery of flavivirus-derived endogenous viral elements in anopheles mosquito genomes supports the existence of anopheles-associated insect-specific flaviviruses. | the flavivirus genus encompasses several arboviruses of public health significance such as dengue, yellow fever, and zika viruses. it also includes insect-specific flaviviruses (isfs) that are only capable of infecting insect hosts. the vast majority of mosquito-infecting flaviviruses have been associated with mosquito species of the aedes and culex genera in the culicinae subfamily, which also includes most arbovirus vectors. mosquitoes of the anophelinae subfamily are not considered significan ... | 2017 | 28078104 |
| functional analysis of the abcs of eye color in helicoverpa armigera with crispr/cas9-induced mutations. | many insect pigments are localized in subcellular pigment granules, and transport of pigment precursors from the cytoplasm is accomplished by abc proteins. drosophila melanogaster has three half-transporter genes (white, scarlet, and brown, all affecting eye pigments) and bombyx mori has a fourth (ok). the white, brown, scarlet and ok proteins each have one transmembrane and one cytoplasmic domain and they heterodimerize to form functional transporters with different substrate specificities. we ... | 2017 | 28053351 |
| evaluations of dual attractant toxic sugar baits for surveillance and control of aedes aegypti and aedes albopictus in florida. | dual attractant toxic sugar baits (d-atsb) containing two host kairomones, l-lactic (la) and 1-octen-3-ol (o), and fruit-based attractants were evaluated through olfactory, consumption and mortality, and semi-field experiments to determine if host kairomones could first, enhance attraction of a fruit-based (attractant) toxic sugar bait (atsb), and second, increase the efficacy of a fruit based attractive toxic sugar bait (atsb). | 2017 | 28057066 |
| evaluation of the importance of fever with respect to dengue prognosis according to the 2009 who classification: a retrospective study. | the 2009 revised world health organization (who) guidelines for dengue describe fever as the core symptom. accordingly, the diagnosis of non-febrile patients is complicated. the aim of this study was to evaluate the importance of fever in patients with dengue according to the 2009 revised who classification. | 2017 | 28052760 |
| enhancement of mosquito trapping efficiency by using pulse width modulated light emitting diodes. | in this study, a light-driving bug zapper is presented for well controlling the diseases brought by insects, such as mosquitoes. in order to have the device efficient to trap the insect pests in off-grid areas, pulse width modulated light emitting diodes (pwm-led) combined with a solar power module are proposed and implemented. with specific pwm electric signals to drive the led, it is found that no matter what the ability of catching insects or the consumed power efficiency can be enhanced thus ... | 2017 | 28059148 |
| the search for novel insecticide targets in the post-genomics era, with a specific focus on g-protein coupled receptors. | insects are considered pests globally, implicated in the destruction of agricultural fields and transmission of pathogens that cause deadly human diseases, such as dengue, zika and malaria. the diversity of the insecticide arsenal has remained stagnant for decades, but the recent rise of insecticide resistance fueled the discovery of novel modes of action, and the power of genomics has reinvigorated this search. this review discusses the importance of comparative and functional insect genomics i ... | 2017 | 28076467 |
| a comparative analysis of three vector-borne diseases across australia using seasonal and meteorological models. | ross river virus (rrv), barmah forest virus (bfv), and dengue are three common mosquito-borne diseases in australia that display notable seasonal patterns. although all three diseases have been modeled on localized scales, no previous study has used harmonic models to compare seasonality of mosquito-borne diseases on a continent-wide scale. we fit poisson harmonic regression models to surveillance data on rrv, bfv, and dengue (from 1993, 1995 and 1991, respectively, through 2015) incorporating s ... | 2017 | 28071683 |
| mitigating future avian malaria threats to hawaiian forest birds from climate change. | avian malaria, transmitted by culex quinquefasciatus mosquitoes in the hawaiian islands, has been a primary contributor to population range limitations, declines, and extinctions for many endemic hawaiian honeycreepers. avian malaria is strongly influenced by climate; therefore, predicted future changes are expected to expand transmission into higher elevations and intensify and lengthen existing transmission periods at lower elevations, leading to further population declines and potential extin ... | 2017 | 28060848 |
| efficient method for establishing f1 progeny from wild populations of anopheles mosquitoes. | the changing malaria situation in madagascar requires additional knowledge on the physiology and behaviour of local mosquito vectors. however, the absence of established colonies for several anopheline species present in madagascar constitutes a limiting factor. to avoid labour intensive work and uncertainty for success of establishing anopheles colonies from malagasy species, field collections of blood-fed females and in-tube forced oviposition were combined to reliably produce large numbers of ... | 2017 | 28069024 |
| commensal viruses of mosquitoes: host restriction, transmission, and interaction with arboviral pathogens. | recent advances in virus detection strategies and deep sequencing technologies have enabled the identification of a multitude of new viruses that persistently infect mosquitoes but do not infect vertebrates. these are usually referred to as insect-specific viruses (isvs). these novel viruses have generated considerable interest in their modes of transmission, persistence in mosquito populations, the mechanisms that restrict their host range to mosquitoes, and their interactions with pathogens tr ... | 2017 | 28096646 |
| persistent zika virus detection in semen in a traveler returning to the united kingdom from brazil, 2016. | zika virus is normally transmitted by mosquitos, but cases of sexual transmission have been reported. we describe a patient with symptomatic zika virus infection in whom the virus was detected in semen for 92 days. our findings support recommendations for 6 months of barrier contraceptive use after symptomatic zika virus infection. | 2017 | 27748650 |
| epidemic outbreak of chikungunya in two neighboring towns in the colombian caribbean: a survival analysis. | the first autochthonous chikungunya virus transmission in colombia was reported in september 2014. three months later, every town in the caribbean region was affected, including the bordering towns of ovejas and corozal, in the department of sucre. the objective of the study was to analyze and compare the temporal dynamics of the outbreak of chikungunya in two towns of the department of sucre. | 2017 | 28074128 |
| insecticidal toxicities of carvacrol and thymol derived from thymus vulgaris lin. against pochazia shantungensis chou &lu., newly recorded pest. | the insecticidal toxicities of five essential oils against pochazia shantungensis adults and nymphs, newly recorded pests, were evaluated. the lc50 values of thymus vulgaris, ruta graveolens, citrus aurantium, leptospermum petersonii and achillea millefolium oils were recorded as 57.48, 84.44, 92.58, 113.26 and 125.78 mg/l, respectively, against p. shantungensis nymphs using the leaf dipping bioassay, and 75.80, 109.86, 113.26, 145.06 and 153.74 mg/l, respectively, against p. shantungensis adult ... | 2017 | 28106093 |
| rna interference mediated knockdown of brugia malayi udp-galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae. | galactofuranose is an essential cell surface component present in bacteria, fungi and several nematodes such as caenorhabditis spp., brugia spp., onchocerca spp. and strongyloides spp. this sugar maintains the integrity of parasite surface and is essential for virulence. udp-galactopyranose mutase (bmugm) plays a key role in galf biosynthesis by catalyzing conversion of udp-galactopyranose into udp-galactofuranose and knockout studies of the gene in leishmania major, mycobacterium and aspergillu ... | 2017 | 28103957 |