Publications

research contributing to improvements in controlling florida's mosquitoes and mosquito-borne diseases.	research on mosquitoes and mosquito-borne diseases has contributed to improvements in providing effective, efficient, and environmentally proper mosquito control. florida has benefitted from several research accomplishments that have increased the state's mosquito control capabilities. research with florida's mosquitoes has resulted in the development of ecologically sound management of mosquito impoundments on florida's east coast. this strategy, called rotational impoundment management (rim), ...	2016	27690112
dengueme: a tool for the modeling and simulation of dengue spatiotemporal dynamics.	the prevention and control of dengue are great public health challenges for many countries, particularly since 2015, as other arboviruses have been observed to interact significantly with dengue virus. different approaches and methodologies have been proposed and discussed by the research community. an important tool widely used is modeling and simulation, which help us to understand epidemic dynamics and create scenarios to support planning and decision making processes. with this aim, we propo ...	2016	27649226
aedes aegypti d7 saliva protein inhibits dengue virus infection.	aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (denv) types 1-4. ae. aegypti transmits denv by inoculating virus-infected saliva into host skin during probing and feeding. ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. previously, we showed that ae. aegypti salivary gland extracts (sges) enhanced dissemination of denv to draining lymph nodes. in con ...	2016	27632170
population genetic structure of aedes fluviatilis (diptera: culicidae).	although aedes fluviatilis is an anthropophilic mosquito found abundantly in urban environments, its biology, epidemiological potential and genetic characteristics are poorly understood. climate change and urbanization processes that result in environmental modifications benefit certain anthropophilic mosquito species such as ae. fluviatilis, greatly increasing their abundance in urban areas. to gain a better understanding of whether urbanization processes modulate the genetic structure of this ...	2016	27598889
the effect of west nile virus infection on the midgut gene expression of culex pipiens quinquefasciatus say (diptera: culicidae).	the interaction of the mosquito and the invading virus is complex and can result in physiological and gene expression alterations in the insect. the association of west nile virus (wnv) and culex pipiens quinquefasciatus mosquitoes results in measurable changes in gene expression; 22 gene products were shown previously to have altered expression. sequence analysis of one product, cq g1a1, revealed 100% amino acid identity to gram negative bacteria binding proteins (cpqgbp) in cx. p. quinquefasci ...	2016	27999244
potential for zika virus to establish a sylvatic transmission cycle in the americas.	zika virus (zikv) originated and continues to circulate in a sylvatic transmission cycle between non-human primate hosts and arboreal mosquitoes in tropical africa. recently zikv invaded the americas, where it poses a threat to human health, especially to pregnant women and their infants. here we examine the risk that zikv will establish a sylvatic cycle in the americas, focusing on brazil. we review the natural history of sylvatic zikv and present a mathematical dynamic transmission model to as ...	2016	27977671
chikungunya virus infection and diabetes mellitus: a double negative impact.	the impact of chikungunya virus (chikv) infection on diabetic patients (dps) has not been described. we aimed to compare clinical features of chikv infection in dps and nondiabetic patients (ndps), and to evaluate its effects on glycemic control among dps. we recorded clinical information and, in dps, glycemic control. forty-six dps and 53 ndps aged ≥ 20 years living in haiti, with acute chikv infection, were studied. diabetes duration was 7.1 ± 6.1 years. the most common acute chikv clinical ma ...	2016	27729569
dengue in the middle east and north africa: a systematic review.	dengue virus (denv) infection is widespread and its disease burden has increased in past decades. however, little is known about the epidemiology of dengue in the middle east and north africa (mena).	2016	27926925
review of west nile virus circulation and outbreak risk in madagascar: entomological and ornithological perspectives.	west nile fever (wnf) is a zoonotic disease, occurring nearly globally. in madagascar, west nile virus (wnv) was first detected in 1978 from wild birds and the virus is currently distributed across the island, but no epidemic or epizootic period has been recorded. one fatal human case of wnv infection was reported in 2011, suggesting a "tip of the iceberg" phenomenon of a possible wnf epidemic/epizootic on the island. the main objective of this literature-based survey is to review patterns of wn ...	2016	27849515
plant virus-insect vector interactions: current and potential future research directions.	acquisition and transmission by an insect vector is central to the infection cycle of the majority of plant pathogenic viruses. plant viruses can interact with their insect host in a variety of ways including both non-persistent and circulative transmission; in some cases, the latter involves virus replication in cells of the insect host. replicating viruses can also elicit both innate and specific defense responses in the insect host. a consistent feature is that the interaction of the virus wi ...	2016	27834855
downregulation of aedes aegypti chromodomain helicase dna binding protein 7/kismet by wolbachia and its effect on dengue virus replication.	dengue virus (denv) is a mosquito-transmitted virus imposing a significant burden on human health around the world. since current control strategies are not sufficient, there is an urgent need to find alternative methods to control denv transmission. it has been demonstrated that introduction of wolbachia pipientis in aedes aegypti mosquitoes can impede denv transmission with the mechanism(s) not fully understood. recently, a number of studies have found the involvement of chromodomain dna bindi ...	2016	27827425
zika virus epidemic in brazil. i. fatal disease in adults: clinical and laboratorial aspects.	zika virus (zikv) was first detected in brazil in may 2015 and the country experienced an explosive epidemic. however, recent studies indicate that the introduction of zikv occurred in late 2013. cases of microcephaly and deaths associated with zikv infection were identified in brazil in november, 2015.	2016	27835759
transmission bottlenecks and rnai collectively influence tick-borne flavivirus evolution.	arthropod-borne rna viruses exist within hosts as heterogeneous populations of viral variants and, as a result, possess great genetic plasticity. understanding the micro-evolutionary forces shaping these viruses can provide insights into how they emerge, adapt, and persist in new and changing ecological niches. while considerable attention has been directed toward studying the population dynamics of mosquito-borne viruses, little is known about tick-borne virus populations. therefore, using a mo ...	2016	28058113
distribution of the members of the pipiens assemblage in the sympatric area from argentina: which is where and when?	given their medical and veterinary relevance, the members of the pipiens assemblage are a worldwide target of ecological research. the distribution of culex pipiens s.s. and cx. quinquefasciatus converge in buenos aires, argentina, where hybrids have been detected. each member of the assemblage exhibits a distinct eco-physiological behaviour that can affect its efficiency in pathogen transmission. our aim was to identify the environmental drivers for the spatio-temporal distribution of each memb ...	2016	27783720
transstadial transmission and long-term association of crimean-congo hemorrhagic fever virus in ticks shapes genome plasticity.	the trade-off hypothesis, the current paradigm of arbovirus evolution, proposes that cycling between vertebrate and invertebrate hosts presents significant constraints on genetic change of arboviruses. studying these constraints in mosquito-borne viruses has led to a new understanding of epizootics. the trade-off hypothesis is assumed to be applicable to tick-borne viruses too, although studies are lacking. tick-borne crimean-congo hemorrhagic fever virus (cchfv), a member of the family bunyavir ...	2016	27775001
combining attractants and larvicides in biodegradable matrices for sustainable mosquito vector control.	there is a global need for cost-effective and environmentally friendly tools for control of mosquitoes and mosquito-borne diseases. one potential way to achieve this is to combine already available tools to gain synergistic effects to reduce vector mosquito populations. another possible way to improve mosquito control is to extend the active period of a given control agent, enabling less frequent applications and consequently, more efficient and longer lasting vector population suppression.	2016	27768698
looking backward, looking forward: the long, torturous struggle with mosquitoes.	the american anti-mosquito movement grew out of the discovery of the role of mosquitoes in transferring pathogens and public concern about pest and nuisance mosquitoes in the late 1800s. in the 20th century, organized mosquito control in the united states passed through three eras: mechanical, chemical, and integrated mosquito control. mosquito control in the 21st century faces the challenge of emerging pathogens, invasive mosquito species, and balancing concerns about the environment with effec ...	2016	27775554
zika antiviral chemotherapy: identification of drugs and promising starting points for drug discovery from an fda-approved library.	background the recent epidemics of zika virus (zikv) implicated it as the cause of serious and potentially lethal congenital conditions such microcephaly and other central nervous system defects, as well as the development of the guillain-barré syndrome in otherwise healthy patients. recent findings showed that anti-dengue antibodies are capable of amplifying zikv infection by a mechanism similar to antibody-dependent enhancement, increasing the severity of the disease. this scenario becomes pot ...	2016	27909576
how did zika virus emerge in the pacific islands and latin america?	the unexpected emergence of zika virus (zikv) in the pacific islands and latin america and its association with congenital zika virus syndrome (czvs) (which includes microcephaly) and guillain-barré syndrome (gbs) have stimulated wide-ranging research. high densities of susceptible aedes spp., immunologically naive human populations, global population growth with increased urbanization, and escalation of global transportation of humans and commercial goods carrying vectors and zikv undoubtedly e ...	2016	27729507
forecasting united states heartworm dirofilaria immitis prevalence in dogs.	this paper forecasts next year's canine heartworm prevalence in the united states from 16 climate, geographic and societal factors. the forecast's construction and an assessment of its performance are described.	2016	27724981
massive yet grossly underestimated global costs of invasive insects.	insects have presented human society with some of its greatest development challenges by spreading diseases, consuming crops and damaging infrastructure. despite the massive human and financial toll of invasive insects, cost estimates of their impacts remain sporadic, spatially incomplete and of questionable quality. here we compile a comprehensive database of economic costs of invasive insects. taking all reported goods and service estimates, invasive insects cost a minimum of us$70.0 billion p ...	2016	27698460
particularities of allergy in the tropics.	allergic diseases are distributed worldwide and their risk factors and triggers vary according to geographical and socioeconomic conditions. allergies are frequent in the tropics but aspects of their prevalence, natural history, risk factors, sensitizers and triggers are not well defined and some are expected to be different from those in temperate zone countries. the aim of this review is to investigate if allergic diseases in the tropics have particularities that deserve special attention for ...	2016	27386040
antinociceptive effect of tephrosia sinapou extract in the acetic acid, phenyl-p-benzoquinone, formalin, and complete freund's adjuvant models of overt pain-like behavior in mice.	tephrosia toxicaria, which is currently known as tephrosia sinapou (buc'hoz) a. chev. (fabaceae), is a source of compounds such as flavonoids. t. sinapou has been used in amazonian countries traditional medicine to alleviate pain and inflammation. the purpose of this study was to evaluate the analgesic effects of t. sinapou ethyl acetate extract in overt pain-like behavior models in mice by using writhing response and flinching/licking tests. we demonstrated in this study that t. sinapou extract ...	2016	27293981
a deep insight into the sialome of male and female aedes aegypti mosquitoes.	only adult female mosquitoes feed on blood, while both genders take sugar meals. accordingly, several compounds associated with blood feeding (i.e. vasodilators, anti-clotting, anti-platelets) are found only in female glands, while enzymes associated with sugar feeding or antimicrobials (such as lysozyme) are found in the glands of both sexes. we performed de novo assembly of reads from adult aedes aegypti female and male salivary gland libraries (285 and 90 million reads, respectively). by mapp ...	2016	26999592
epidemiology of chikungunya virus in bahia, brazil, 2014-2015.	chikungunya is an emerging arbovirus that is characterized into four lineages. one of these, the asian genotype, has spread rapidly in the americas after its introduction in the saint martin island in october 2013. unexpectedly, a new lineage, the east-central-south african genotype, was introduced from angola in the end of may 2014 in feira de santana (fsa), the second largest city in bahia state, brazil, where over 5,500 cases have now been reported. number weekly cases of clinically confirmed ...	2016	27330849
accuracy of clinical criteria and an immunochromatographic strip test for dengue diagnosis in a denv-4 epidemic.	early diagnosis of dengue infection is important for decision-making and timely implementation of therapeutic measures. although rapid ns1 assays have been used for dengue diagnosis since 2008, their performance in denv-4 cases has not yet been fully assessed.	2016	26822788
in silico analysis of an envelope domain iii-based multivalent fusion protein as a potential dengue vaccine candidate.	dengue virus infection is now a global problem. currently, there is no licensed vaccine or proven antiviral treatment against this virus. all four serotypes (1-4) of dengue virus can infect human. an effective dengue vaccine should be tetravalent to induce protective immune responses against all four serotypes. most of dengue vaccine candidates are monovalent, or in the form of physically mixed multivalent formulations. recently envelope protein domain iii of virus is considered as a vaccine can ...	2016	26866023
the risk of dengue virus transmission in dar es salaam, tanzania during an epidemic period of 2014.	in 2010, 2012, 2013 and 2014 dengue outbreaks have been reported in dar es salaam, tanzania. however, there is no comprehensive data on the risk of transmission of dengue in the country. the objective of this study was to assess the risk of transmission of dengue in dar es salaam during the 2014 epidemic.	2016	26812489
identification of proteins bound to dengue viral rna in vivo reveals new host proteins important for virus replication.	dengue virus is the most prevalent cause of arthropod-borne infection worldwide. due to the limited coding capacity of the viral genome and the complexity of the viral life cycle, host cell proteins play essential roles throughout the course of viral infection. host rna-binding proteins mediate various aspects of virus replication through their physical interactions with viral rna. here we describe a technique designed to identify such interactions in the context of infected cells using uv cross ...	2016	26733069
relevance of non-communicable comorbidities for the development of the severe forms of dengue: a systematic literature review.	patients with dengue fever and comorbidities seem to be at higher risk of developing complications and/or severe dengue compared to healthier individuals. this study systematically reviews the evidence related to comorbidities and dengue. a systematic literature review was performed in five databases (embase, pubmed, global health, scielo, cochrane) and grey literature for full-text articles since its inceptions until october 10, 2015. a total of 230 articles were retrieved. sixteen studies were ...	2016	26727113
antibody-dependent enhancement of dengue virus infection inhibits rlr-mediated type-i ifn-independent signalling through upregulation of cellular autophagy.	antibody dependent enhancement (ade) of dengue virus (denv) infection is identified as the main risk factor of severe dengue diseases. through opsonization by subneutralizing or non-neutralizing antibodies, denv infection suppresses innate cell immunity to facilitate viral replication. however, it is largely unknown whether suppression of type-i ifn is necessary for a successful ade infection. here, we report that both denv and denv-ade infection induce an early isg (nos2) expression through rlr ...	2016	26923481
in silico screening, alanine mutation, and dft approaches for identification of ns2b/ns3 protease inhibitors.	to identify the ligand that binds to a target protein with high affinity is a nontrivial task in computer-assisted approaches. antiviral drugs have been identified for ns2b/ns3 protease enzyme on the mechanism to cleave the viral protein using the computational tools. the consequence of the molecular docking, free energy calculations, and simulation protocols explores the better ligand. it provides in-depth structural insights with the catalytic triad of his51, asp75, ser135, and gly133. the md ...	2016	27057355
no evidence for local adaptation of dengue viruses to mosquito vector populations in thailand.	despite their epidemiological importance, the evolutionary forces that shape the spatial structure of dengue virus genetic diversity are not fully understood. fine-scale genetic structure of mosquito vector populations and evidence for genotype × genotype interactions between dengue viruses and their mosquito vectors are consistent with the hypothesis that the geographical distribution of dengue virus genetic diversity may reflect viral adaptation to local mosquito populations. to test this hypo ...	2016	27099625
phylogenetic analysis of dengue virus 1 isolated from south minas gerais, brazil.	dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. primary infection with a single dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. however, a subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease, called dengue hemorrhagic fever. the four dengue virus serotypes (1-4) are antigenically and genetically distinct and each ...	2016	26887252
projected impact of dengue vaccination in yucatán, mexico.	dengue vaccines will soon provide a new tool for reducing dengue disease, but the effectiveness of widespread vaccination campaigns has not yet been determined. we developed an agent-based dengue model representing movement of and transmission dynamics among people and mosquitoes in yucatán, mexico, and simulated various vaccine scenarios to evaluate effectiveness under those conditions. this model includes detailed spatial representation of the yucatán population, including the location and mov ...	2016	27227883
temporal dynamics and spatial patterns of aedes aegypti breeding sites, in the context of a dengue control program in tartagal (salta province, argentina).	since 2009, fundación mundo sano has implemented an aedes aegypti surveillance and control program in tartagal city (salta province, argentina). the purpose of this study was to analyze temporal dynamics of ae. aegypti breeding sites spatial distribution, during five years of samplings, and the effect of control actions over vector population dynamics.	2016	27223693
meta-analysis of dengue severity during infection by different dengue virus serotypes in primary and secondary infections.	dengue virus (denv) infection is currently a major cause of morbidity and mortality in the world; it has become more common and virulent over the past half-century and has gained much attention. thus, this review compared the percentage of severe cases of both primary and secondary infections with different serotypes of dengue virus.	2016	27213782
the risk of dengue for non-immune foreign visitors to the 2016 summer olympic games in rio de janeiro, brazil.	rio de janeiro in brazil will host the summer olympic games in 2016. about 400,000 non-immune foreign tourists are expected to attend the games. as brazil is the country with the highest number of dengue cases worldwide, concern about the risk of dengue for travelers is justified.	2016	27129407
analysis of significant factors for dengue fever incidence prediction.	many popular dengue forecasting techniques have been used by several researchers to extrapolate dengue incidence rates, including the k-h model, support vector machines (svm), and artificial neural networks (ann). the time series analysis methodology, particularly arima and sarima, has been increasingly applied to the field of epidemiological research for dengue fever, dengue hemorrhagic fever, and other infectious diseases. the main drawback of these methods is that they do not consider other v ...	2016	27083696
first chikungunya outbreak in suriname; clinical and epidemiological features.	in june 2014, suriname faced the first chikungunya outbreak. since international reports mostly focus on hospitalized patients, the least affected group, a study was conducted to describe clinical characteristics of mainly outpatients including children. in addition, the cumulative incidence of this first epidemic was investigated.	2016	27082985
assessing potential countermeasures against the dengue epidemic in non-tropical urban cities.	dengue is a common mosquito-borne viral disease epidemic especially in tropical and sub-tropical regions where water sanitation is not substantially controlled. however, dengue epidemics sometimes occur in non-tropical urban cities with substantial water sanitary control. using a mathematical model, we investigate what conditions can be important for a dengue epidemic to occur in an urban city such as tokyo, where vectors are active only in summer and there are little number of vectors around ho ...	2016	27072122
epidemiological dynamics of an urban dengue 4 outbreak in são paulo, brazil.	dengue studies at the urban scale are scarce and required for guiding control efforts. in brazil, the burden of dengue is high and challenges city public health administrations with limited resources. here we studied the dynamics of a dengue epidemic in a single city.	2016	27069820
presence of three dengue serotypes in ouagadougou (burkina faso): research and public health implications.	the significant malaria burden in africa has often eclipsed other febrile illnesses. burkina faso's first dengue epidemic occurred in 1925 and the most recent in 2013. yet there is still very little known about dengue prevalence, its vector proliferation, and its poverty and equity impacts.	2016	27044528
fitting the incidence data from the city of campinas, brazil, based on dengue transmission modellings considering time-dependent entomological parameters.	four time-dependent dengue transmission models are considered in order to fit the incidence data from the city of campinas, brazil, recorded from october 1st 1995 to september 30th 2012. the entomological parameters are allowed to depend on temperature and precipitation, while the carrying capacity and the hatching of eggs depend only on precipitation. the whole period of incidence of dengue is split into four periods, due to the fact that the model is formulated considering the circulation of o ...	2016	27010654
evolutionary analysis of dengue serotype 2 viruses using phylogenetic and bayesian methods from new delhi, india.	dengue fever is the most important arboviral disease in the tropical and sub-tropical countries of the world. delhi, the metropolitan capital state of india, has reported many dengue outbreaks, with the last outbreak occurring in 2013. we have recently reported predominance of dengue virus serotype 2 during 2011-2014 in delhi. in the present study, we report molecular characterization and evolutionary analysis of dengue serotype 2 viruses which were detected in 2011-2014 in delhi. envelope genes ...	2016	26977703
dengue in java, indonesia: relevance of mosquito indices as risk predictors.	no vaccine is currently available for dengue virus (denv), therefore control programmes usually focus on managing mosquito vector populations. entomological surveys provide the most common means of characterising vector populations and predicting the risk of local dengue virus transmission. despite indonesia being a country strongly affected by denv, only limited information is available on the local factors affecting denv transmission and the suitability of available survey methods for assessin ...	2016	26967524
co-distribution and co-infection of chikungunya and dengue viruses.	chikungunya and dengue infections are spatio-temporally related. the current review aims to determine the geographic limits of chikungunya, dengue and the principal mosquito vectors for both viruses and to synthesise current epidemiological understanding of their co-distribution.	2016	26936191
comparative analysis of dengue and zika outbreaks reveals differences by setting and virus.	the pacific islands of micronesia have experienced several outbreaks of mosquito-borne diseases over the past decade. in outbreaks on small islands, the susceptible population is usually well defined, and there is no co-circulation of pathogens. because of this, analysing such outbreaks can be useful for understanding the transmission dynamics of the pathogens involved, and particularly so for yet understudied pathogens such as zika virus. here, we compared three outbreaks of dengue and zika vir ...	2016	27926933
aedes (stegomyia) albopictus' dynamics influenced by spatiotemporal characteristics in a brazilian dengue-endemic risk city.	brazil reported the majority of the dengue cases in americas during the last two decades, where the occurrence of human dengue cases is exclusively attributed to the aedes (stegomyia) aegypti (linnaeus). nowadays, other recognized dengue virus (denv) vector in asian countries, aedes (stegomyia) albopictus (skuse), has been detected in more than half of the 5565 brazilian municipalities. therefore, the aim of the present study was to investigate the presence of, and determine the ae. albopictus' ...	2016	27771419
analysis of synonymous codon usage bias of zika virus and its adaption to the hosts.	zika virus (zikv) is a mosquito-borne virus (arbovirus) in the family flaviviridae, and the symptoms caused by zikv infection in humans include rash, fever, arthralgia, myalgia, asthenia and conjunctivitis. codon usage bias analysis can reveal much about the molecular evolution and host adaption of zikv. to gain insight into the evolutionary characteristics of zikv, we performed a comprehensive analysis on the codon usage pattern in 46 zikv strains by calculating the effective number of codons ( ...	2016	27893824
a prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting.	dengue is one of the most important vector-borne diseases in the world, causing significant morbidity and economic impact. in colombia, dengue is a major public health problem. departments of la guajira, cesar and magdalena are dengue endemic areas. the objective of this research is to determine the seroprevalence and the incidence of dengue virus infection in the participating municipalities from these departments, and also establish the association between individual and housing factors and ve ...	2016	27887591
tsetse fly tolerance to t. brucei infection: transcriptome analysis of trypanosome-associated changes in the tsetse fly salivary gland.	for their transmission, african trypanosomes rely on their blood feeding insect vector, the tsetse fly (glossina sp.). the ingested trypanosoma brucei parasites have to overcome a series of barriers in the tsetse fly alimentary tract to finally develop into the infective metacyclic forms in the salivary glands that are transmitted to a mammalian host by the tsetse bite. the parasite population in the salivary gland is dense with a significant number of trypanosomes tightly attached to the epithe ...	2016	27884110
susceptibility profile and metabolic mechanisms involved in aedes aegypti and aedes albopictus resistant to ddt and deltamethrin in the central african republic.	aedes aegypti and ae. albopictus are the main epidemic vectors of dengue, chikungunya and zika viruses worldwide. their control during epidemics relies mainly on control of larvae and adults with insecticides. unfortunately, loss of susceptibility of both species to several insecticide classes limits the efficacy of interventions. in africa, where aedes-borne viruses are of growing concern, few data are available on resistance to insecticides. to fill this gap, we assessed the susceptibility to ...	2016	27881148
generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization.	due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (denv), chikungunya and zika virus, have developed into major public health challenges. since all of these viruses may cause similar symptoms and can occur in concurrent epidemics, tools for their differential diagnosis and epidemiological monitoring are of urgent need.	2016	27876044
the driving force for 2014 dengue outbreak in guangdong, china.	dengue fever has rapidly spread in recent decades to become the most globally expansive viral vector-borne disease. in mainland china, a number of dengue outbreaks have been reported since 1978, but the worst epidemic in decades, involving 45230 cases and 76 imported cases, resulting in six deaths in guangdong province, emerged in 2014. reasons for this ongoing surge in dengue, both imported and autochthonous, are currently unclear and demand urgent investigation. here, a seasonally-driven dynam ...	2016	27861514
genomic studies of envelope gene sequences from mosquito and human samples from bangkok, thailand.	dengue virus (denv) is an rna virus showing a high degree of genetic variation as a consequence of its proofreading inability. this variation plays an important role in virus evolution and pathogenesis. although levels of within-host genetic variation are similar following equilibrium, variation among different hosts is frequently different. to identify dengue quasispecies present among two hosts, we collected patient samples from six acute denv cases and two pools of aedes aegypti mosquitoes an ...	2016	27917342
detection of dengue virus serotypes 1, 2 and 3 in selected regions of kenya: 2011-2014.	dengue fever, a mosquito-borne disease, is associated with illness of varying severity in countries in the tropics and sub tropics. dengue cases continue to be detected more frequently and its geographic range continues to expand. we report the largest documented laboratory confirmed circulation of dengue virus in parts of kenya since 1982.	2016	27814732
comprehensive dna methylation analysis of the aedes aegypti genome.	aedes aegypti mosquitoes are important vectors of viral diseases. mosquito host factors play key roles in virus control and it has been suggested that dengue virus replication is regulated by dnmt2-mediated dna methylation. however, recent studies have shown that dnmt2 is a trna methyltransferase and that dnmt2-dependent methylomes lack defined dna methylation patterns, thus necessitating a systematic re-evaluation of the mosquito genome methylation status. we have now searched the ae. aegypti g ...	2016	27805064
dengue outbreak in mombasa city, kenya, 2013-2014: entomologic investigations.	dengue outbreaks were first reported in east africa in the late 1970s to early 1980s including the 1982 outbreak on the kenyan coast. in 2011, dengue outbreaks occurred in mandera in northern kenya and subsequently in mombasa city along the kenyan coast in 2013-2014. following laboratory confirmation of dengue fever cases, an entomologic investigation was conducted to establish the mosquito species, and densities, causing the outbreak. affected parts of the city were identified with the help of ...	2016	27783626
the interplay of vaccination and vector control on small dengue networks.	dengue fever is a major public health issue affecting billions of people in over 100 countries across the globe. this challenge is growing as the invasive mosquito vectors, aedes aegypti and aedes albopictus, expand their distributions and increase their population sizes. hence there is an increasing need to devise effective control methods that can contain dengue outbreaks. here we construct an epidemiological model for virus transmission between vectors and hosts on a network of host populatio ...	2016	27457093
zika mosquito vectors: the jury is still out.	after a 40-year hiatus, the international congress of entomology (ice 2016) convened in orlando, florida (september 25-30, 2016). one of the symposia at ice 2016, the zika symposium, covered multiple aspects of the zika epidemic, including epidemiology, sexual transmission, genetic tools for reducing transmission, and particularly vector competence. while there was a consensus among participants that the yellow fever mosquito, aedes aegypti, is a vector of the zika virus, there is growing eviden ...	2016	27853521
polyphenol-rich diets exacerbate ampk-mediated autophagy, decreasing proliferation of mosquito midgut microbiota, and extending vector lifespan.	mosquitoes feed on plant-derived fluids such as nectar and sap and are exposed to bioactive molecules found in this dietary source. however, the role of such molecules on mosquito vectorial capacity is unknown. weather has been recognized as a major determinant of the spread of dengue, and plants under abiotic stress increase their production of polyphenols.	2016	27732590
emergence of epidemic dengue-1 virus in the southern province of sri lanka.	dengue is a frequent cause of acute febrile illness with an expanding global distribution. since the 1960s, dengue in sri lanka has been documented primarily along the heavily urbanized western coast with periodic shifting of serotypes. outbreaks from 2005-2008 were attributed to a new clade of denv-3 and more recently to a newly introduced genotype of denv-1. in 2007, we conducted etiologic surveillance of acute febrile illness in the southern province and confirmed dengue in only 6.3% of febri ...	2016	27711206
epidemiological and molecular characterization of dengue viruses imported into guangzhou during 2009-2013.	dengue virus causes one of the most significant infectious diseases in tropical and subtropical regions, notable number of which is imported into china every year.	2016	27722053
epidemiologic investigation of a family cluster of imported zikv cases in guangdong, china: probable human-to-human transmission.	zika virus (zikv) is an emerging mosquito-borne flavivirus that can potentially threaten south china. a chinese family of four returning from venezuela to china was found to be positive for zikv when the youngest son's fever was first detected at an airport immigration inspection. they were isolated temporarily in a local hospital in enping city, guangdong province, where their clinical data were recorded and urine and saliva were collected to isolate zikv and to obtain viral sequences. all of t ...	2016	27599469
the importance of socio-economic versus environmental risk factors for reported dengue cases in java, indonesia.	dengue is a major mosquito-borne viral disease and an important public health problem. identifying which factors are important determinants in the risk of dengue infection is critical in supporting and guiding preventive measures. in south-east asia, half of all reported fatal infections are recorded in indonesia, yet little is known about the epidemiology of dengue in this country.	2016	27603137
maintenance of demographic and hematological profiles in a long-lasting dengue fever outbreak: implications for management.	dengue fever (df) outbreaks present regionally specific epidemiological and clinical characteristics. in certain medium-sized cities (100 000-250 000 inhabitants) of são paulo state, brazil, and after reaching an incidence of 150 cases/100 000 inhabitants ("epidemiological threshold"), clinical diagnosis indicated dengue virus (denv) infection. during this period, other seasonally infectious diseases with symptoms and physical signs mimicking df can simultaneously occur, with the consequential o ...	2016	27593529
measuring haitian children's exposure to chikungunya, dengue and malaria.	to differentiate exposure to the newly introduced chikungunya virus from exposure to endemic dengue virus and other pathogens in haiti.	2016	27821884
patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue.	in the present study, the construction of arrays on silicon for naked-eye detection of dna dengue was demonstrated. the array was created by exposing a polyethylene glycol (peg) silane monolayer to 254 nm ultraviolet (uv) light through a photomask. formation of the peg silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (afm), x-ray photoelectron spectroscopy (xps), and contact angle measurements. the results of xps confirmed that ir ...	2016	27571080
impact of dengue virus (denv) co-infection on clinical manifestations, disease severity and laboratory parameters.	the co-circulation of 4 denv serotypes in geographically expanding area, has resulted in increasing occurrence of denv co-infections. however, studies assessing the clinical impact of denv co-infections have been scarce and have involved small number of patients. this study explores the impact of denv co-infection on clinical manifestations and laboratory parameters.	2016	27514512
extended evaluation of virological, immunological and pharmacokinetic endpoints of celaden: a randomized, placebo-controlled trial of celgosivir in dengue fever patients.	celaden was a randomized placebo-controlled trial of 50 patients with confirmed dengue fever to evaluate the efficacy and safety of celgosivir (a study registered at clinicaltrials.gov, number nct01619969). celgosivir was given as a 400 mg loading dose and 200 mg bid (twice a day) over 5 days. replication competent virus was measured by plaque assay and compared to reverse transcription quantitative pcr (qpcr) of viral rna. pharmacokinetics (pk) correlations with viremia, immunological profiling ...	2016	27509020
prospects for dengue vaccines for travelers.	travel-acquired dengue cases have been increasing as the overall global dengue burden has expanded. in korea, imported dengue cases have been reported since 2000 when it first became a notifiable disease. during the first four months of 2016, three times more dengue cases were reported in korea than during the same period the previous year. a safe and efficacious vaccine for travelers would be beneficial to prevent dengue disease in individual travelers and potentially decrease the risk of virus ...	2016	27489798
storm drains as larval development and adult resting sites for aedes aegypti and aedes albopictus in salvador, brazil.	dengue (denv), chikungunya (chikv), zika (zikv), as well as yellow fever (yfv) viruses are transmitted to humans by aedes spp. females. in salvador, the largest urban center in north-eastern brazil, the four denv types have been circulating, and more recently, chikv and zikv have also become common. we studied the role of storm drains as aedes larval development and adult resting sites in four neighbourhoods of salvador, representing different socioeconomic, infrastructure and topographic condit ...	2016	27464886
effect of spatial repellent exposure on dengue vector attraction to oviposition sites.	aedes aegypti is a primary vector of dengue virus (denv), the causative agent of dengue fever, an arthropod-borne disease of global importance. although a vaccine has been recommended for prevention, current dengue prevention strategies rely on vector control. recently, volatile pyrethroids-spatial repellents-have received interest as a novel delivery system for adult ae. aegypti control. understanding the full range of behavioral effects spatial repellents elicit in mosquito species will be cri ...	2016	27428011
zika virus challenges for neuropsychiatry.	before 2007, zika virus (zikv) was generally considered as an arbovirus of low clinical relevance, causing a mild self-limiting febrile illness in tropical africa and southeast asia. currently, a large, ongoing outbreak of zikv that started in brazil in 2015 is spreading across the americas. virus infection during pregnancy has been potentially linked to congenital malformations, including microcephaly. in addition to congenital malformations, a temporal association between zikv infection and an ...	2016	27478378
applications of a rapid and sensitive dengue duo rapid immunochromatographic test kit as a diagnostic strategy during a dengue type 2 epidemic in an urban city.	dengue infection is a major health problem in tropical and subtropical countries. a prospective observational study in a university-affiliated hospital was conducted between august 2015 and september 2015. patients who visited the emergency department (ed) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (ns1), igm/igg rapid immunochromatographic tests and real-time polymerase chain reaction (rt-pcr) to evaluate the performance of the rapid test ...	2016	27415767
serotype influences on dengue severity: a cross-sectional study on 485 confirmed dengue cases in vitória, brazil.	dengue is caused by a rna virus of the family flaviviridae, which presents four serotypes (denv-1 to denv-4) capable of inducing hemorrhage. the purpose of this study was to evaluate the influence of serotype on the outcome of dengue.	2016	27393011
dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus.	zika virus (zikv) was discovered in 1947 and was thought to lead to relatively mild disease. the recent explosive outbreak of zikv in south america has led to widespread concern, with reports of neurological sequelae ranging from guillain barré syndrome to microcephaly. zikv infection has occurred in areas previously exposed to dengue virus (denv), a flavivirus closely related to zikv. here we investigated the serological cross-reaction between the two viruses. plasma immune to denv showed subst ...	2016	27339099
epidemic resurgence of dengue fever in singapore in 2013-2014: a virological and entomological perspective.	dengue resurged in singapore during 2013-14, causing an outbreak with unprecedented number of cases in the country. in the present study, we summarise the epidemiological, virological and entomological findings gathered through the dengue surveillance programme and highlight the drivers of the epidemic. we also describe how the surveillance system facilitated the preparedness to moderate epidemic transmission of dengue in the country.	2016	27316694
virus-like particles derived from pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain iii-directed antibodies.	four antigenically distinct serotypes (1-4) of dengue viruses (denvs) cause dengue disease. antibodies to any one denv serotype have the potential to predispose an individual to more severe disease upon infection with a different denv serotype. a dengue vaccine must elicit homotypic neutralizing antibodies to all four denv serotypes to avoid the risk of such antibody-dependent enhancement in the vaccine recipient. this is a formidable challenge as evident from the lack of protective efficacy aga ...	2016	27301568
assessing seasonal risks for the introduction and mosquito-borne spread of zika virus in europe.	the explosive zika virus epidemic in the americas is amplifying spread of this emerging pathogen into previously unaffected regions of the world, including europe (gulland, 2016), where local populations are immunologically naïve. as summertime approaches in the northern hemisphere, aedes mosquitoes in europe may find suitable climatic conditions to acquire and subsequently transmit zika virus from viremic travellers to local populations. while aedes albopictus has proven to be a vector for the ...	2016	27344225
population genetics of the asian tiger mosquito aedes albopictus, an invasive vector of human diseases.	the asian tiger mosquito aedes albopictus is currently one of the most threatening invasive species in the world. native to southeast asia, the species has spread throughout the world in the past 30 years and is now present in every continent but antarctica. because it was the main vector of recent dengue and chikungunya outbreaks, and because of its competency for numerous other viruses and pathogens such as the zika virus, a. albopictus stands out as a model species for invasive diseases vecto ...	2016	27273325
the influence of urbanization modes on the spatial circulation of flaviviruses within ouagadougou (burkina faso).	dengue is an emerging infectious disease of global significance. although this virus has been reported for a long time, its significance within the burden of diseases in west africa is not obvious, especially in burkina faso. our objective was to evaluate flavivirus presence in ouagadougou (burkina faso) and the link between anti-flavivirus antibody seroprevalence and urbanization modes. a population-based cross-sectional survey was conducted and 3015 children were enrolled from ouagadougou dist ...	2016	27973402
detection of human anti-zika virus igg by elisa using an antigen from in vitro infected vero cells: preliminary results.	zika virus (zkv) infection is a huge public health problem in brazil because of the increased incidence of microcephaly in neonates from infected mothers. detection of specific igg antibodies in maternal serum samples constitutes an important approach for diagnosing zkv infection and evaluating its relationship with neonatal microcephaly. however, as there is no serological test produced in brazil to detect igm and igg antibodies against zkv, we sought to examine specific igg in serum samples fr ...	2016	27982355
invasive mutualisms between a plant pathogen and insect vectors in the middle east and brazil.	complex multi-trophic interactions in vectorborne diseases limit our understanding and ability to predict outbreaks. arthropod-vectored pathogens are especially problematic, with the potential for novel interspecific interactions during invasions. variations and novelties in plant-arthropod-pathogen triumvirates present significant threats to global food security. we examined aspects of a phytoplasma pathogen of citrus across two continents. 'candidatus phytoplasma aurantifolia' causes witches' ...	2016	28083099
evaluations of dual attractant toxic sugar baits for surveillance and control of aedes aegypti and aedes albopictus in florida.	dual attractant toxic sugar baits (d-atsb) containing two host kairomones, l-lactic (la) and 1-octen-3-ol (o), and fruit-based attractants were evaluated through olfactory, consumption and mortality, and semi-field experiments to determine if host kairomones could first, enhance attraction of a fruit-based (attractant) toxic sugar bait (atsb), and second, increase the efficacy of a fruit based attractive toxic sugar bait (atsb).	2017	28057066
evaluation of the importance of fever with respect to dengue prognosis according to the 2009 who classification: a retrospective study.	the 2009 revised world health organization (who) guidelines for dengue describe fever as the core symptom. accordingly, the diagnosis of non-febrile patients is complicated. the aim of this study was to evaluate the importance of fever in patients with dengue according to the 2009 revised who classification.	2017	28052760
enhancement of mosquito trapping efficiency by using pulse width modulated light emitting diodes.	in this study, a light-driving bug zapper is presented for well controlling the diseases brought by insects, such as mosquitoes. in order to have the device efficient to trap the insect pests in off-grid areas, pulse width modulated light emitting diodes (pwm-led) combined with a solar power module are proposed and implemented. with specific pwm electric signals to drive the led, it is found that no matter what the ability of catching insects or the consumed power efficiency can be enhanced thus ...	2017	28059148
the search for novel insecticide targets in the post-genomics era, with a specific focus on g-protein coupled receptors.	insects are considered pests globally, implicated in the destruction of agricultural fields and transmission of pathogens that cause deadly human diseases, such as dengue, zika and malaria. the diversity of the insecticide arsenal has remained stagnant for decades, but the recent rise of insecticide resistance fueled the discovery of novel modes of action, and the power of genomics has reinvigorated this search. this review discusses the importance of comparative and functional insect genomics i ...	2017	28076467
a comparative analysis of three vector-borne diseases across australia using seasonal and meteorological models.	ross river virus (rrv), barmah forest virus (bfv), and dengue are three common mosquito-borne diseases in australia that display notable seasonal patterns. although all three diseases have been modeled on localized scales, no previous study has used harmonic models to compare seasonality of mosquito-borne diseases on a continent-wide scale. we fit poisson harmonic regression models to surveillance data on rrv, bfv, and dengue (from 1993, 1995 and 1991, respectively, through 2015) incorporating s ...	2017	28071683
mitigating future avian malaria threats to hawaiian forest birds from climate change.	avian malaria, transmitted by culex quinquefasciatus mosquitoes in the hawaiian islands, has been a primary contributor to population range limitations, declines, and extinctions for many endemic hawaiian honeycreepers. avian malaria is strongly influenced by climate; therefore, predicted future changes are expected to expand transmission into higher elevations and intensify and lengthen existing transmission periods at lower elevations, leading to further population declines and potential extin ...	2017	28060848
efficient method for establishing f1 progeny from wild populations of anopheles mosquitoes.	the changing malaria situation in madagascar requires additional knowledge on the physiology and behaviour of local mosquito vectors. however, the absence of established colonies for several anopheline species present in madagascar constitutes a limiting factor. to avoid labour intensive work and uncertainty for success of establishing anopheles colonies from malagasy species, field collections of blood-fed females and in-tube forced oviposition were combined to reliably produce large numbers of ...	2017	28069024
commensal viruses of mosquitoes: host restriction, transmission, and interaction with arboviral pathogens.	recent advances in virus detection strategies and deep sequencing technologies have enabled the identification of a multitude of new viruses that persistently infect mosquitoes but do not infect vertebrates. these are usually referred to as insect-specific viruses (isvs). these novel viruses have generated considerable interest in their modes of transmission, persistence in mosquito populations, the mechanisms that restrict their host range to mosquitoes, and their interactions with pathogens tr ...	2017	28096646
persistent zika virus detection in semen in a traveler returning to the united kingdom from brazil, 2016.	zika virus is normally transmitted by mosquitos, but cases of sexual transmission have been reported. we describe a patient with symptomatic zika virus infection in whom the virus was detected in semen for 92 days. our findings support recommendations for 6 months of barrier contraceptive use after symptomatic zika virus infection.	2017	27748650
epidemic outbreak of chikungunya in two neighboring towns in the colombian caribbean: a survival analysis.	the first autochthonous chikungunya virus transmission in colombia was reported in september 2014. three months later, every town in the caribbean region was affected, including the bordering towns of ovejas and corozal, in the department of sucre. the objective of the study was to analyze and compare the temporal dynamics of the outbreak of chikungunya in two towns of the department of sucre.	2017	28074128
insecticidal toxicities of carvacrol and thymol derived from thymus vulgaris lin. against pochazia shantungensis chou &lu., newly recorded pest.	the insecticidal toxicities of five essential oils against pochazia shantungensis adults and nymphs, newly recorded pests, were evaluated. the lc50 values of thymus vulgaris, ruta graveolens, citrus aurantium, leptospermum petersonii and achillea millefolium oils were recorded as 57.48, 84.44, 92.58, 113.26 and 125.78 mg/l, respectively, against p. shantungensis nymphs using the leaf dipping bioassay, and 75.80, 109.86, 113.26, 145.06 and 153.74 mg/l, respectively, against p. shantungensis adult ...	2017	28106093
rna interference mediated knockdown of brugia malayi udp-galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae.	galactofuranose is an essential cell surface component present in bacteria, fungi and several nematodes such as caenorhabditis spp., brugia spp., onchocerca spp. and strongyloides spp. this sugar maintains the integrity of parasite surface and is essential for virulence. udp-galactopyranose mutase (bmugm) plays a key role in galf biosynthesis by catalyzing conversion of udp-galactopyranose into udp-galactofuranose and knockout studies of the gene in leishmania major, mycobacterium and aspergillu ...	2017	28103957
antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections.	the transmission of dengue virus (denv) and chikungunya virus (chikv) has increased worldwide, due in part to the lack of a specific antiviral treatment. for this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. the objective of this study was to identify some of the compounds that are present in mammea americana (m. americana) and tabernaemontana cymosa (t. cymosa) plants and, subsequently, to evaluate their cyto ...	2017	28100218
microrna-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.	micrornas (mirnas) are key regulators of developmental processes, such as cell fate determination and differentiation. previous studies showed dicer knockdown in honeybee embryos disrupt the processing of functional mature mirnas and impairs embryo patterning. here we investigated the expression profiles of mirnas in honeybee embryogenesis and the role of the highly conserved mir-34-5p in the regulation of genes involved in insect segmentation. a total of 221 mirnas were expressed in honey bee e ...	2017	28098233
assessment of synthetic floral-based attractants and sugar baits to capture male and female aedes aegypti (diptera: culicidae).	the viruses transmitted by aedes aegypti, including dengue and zika viruses, are rapidly expanding in geographic range and as a threat to public health. in response, control programs are increasingly turning to the use of sterile insect techniques resulting in a need to trap male ae. aegypti to monitor the efficacy of the intervention. however, there is a lack of effective and cheap methods for trapping males. thus, we attempted to exploit the physiological need to obtain energy from sugar feedi ...	2017	28095875