Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| biofilm synthesis and presence of virulence factors among enterococci isolated from patients and water samples. | the goal of this study was to compare biofilm synthesis among enterococci recovered from clinical samples (infection or colonization) of patients as well as environmental samples in order to determine possible virulence factors and clonal relationship. during a two-year period, clinical samples (blood, catheter tips, bronchial secretions, wounds, peritoneal fluid, urine) and rectal swabs collected from hospitalized patients as well as environmental water samples were tested for the presence of e ... | 2015 | 26242895 |
| identification of vancomycin interaction with enterococcus faecalis within 30 min of interaction time using raman spectroscopy. | vancomycin is an important glycopeptide antibiotic which is used to treat serious infections caused by gram-positive bacteria. however, during the last years, a tremendous rise in vancomycin resistances, especially among enterococci, was reported, making fast diagnostic methods inevitable. in this contribution, we apply raman spectroscopy to systematically characterize vancomycin-enterococci interactions over a time span of 90 min using a sensitive enterococcus faecalis strain and two different ... | 2015 | 26231687 |
| the in vitro effect of irrigants with low surface tension on enterococcus faecalis. | due to the complex anatomy of the root canal system and high surface tension of common root canal irrigants (rci), conducting an investigation on rcis containing surfactants is a priority. the aim of this in vitro study was to verify the antibacterial potential of rci with low surface tension in root canals infected with enterococcus faecalis (e. faecalis). | 2015 | 26229541 |
| the enterococcal cytolysin synthetase has an unanticipated lipid kinase fold. | the enterococcal cytolysin is a virulence factor consisting of two post-translationally modified peptides that synergistically kill human immune cells. both peptides are made by cylm, a member of the lanm lanthipeptide synthetases. cylm catalyzes seven dehydrations of ser and thr residues and three cyclization reactions during the biosynthesis of the cytolysin large subunit. we present here the 2.2 å resolution structure of cylm, the first structural information on a lanm. unexpectedly, the stru ... | 2015 | 26226635 |
| leukocyte presence does not increase microbicidal activity of platelet-rich plasma in vitro. | human platelets are a rich reservoir of molecules that promote regenerative processes and microbicidal activity. this activity might be increased by concentration in platelet-rich plasma (prp) products and modulated by the presence of leukocytes. despite extensive use in clinical procedures, only few studies have investigated prp's real microbicidal potential. therefore, this study aimed at comparing the in vitro microbicidal activity of platelets and leukocyte-enriched prp (l-prp) to pure plate ... | 2015 | 26223356 |
| surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic escherichia coli biofilms against pathogen colonization. | prevention of pathogenic colonization on medical devices over a long period of time remains a great challenge, especially in a high-nutrient environment that accelerates the production of biomass leading to biofouling of the device. since biofouling and the subsequent pathogen colonization is eventually inevitable, a new strategy using non-pathogenic bacteria as living guards against pathogenic colonization on medical devices has attracted increasing interest. crucial to the success of this stra ... | 2015 | 26221844 |
| epidemiological alteration in pathogens found in ground meat in iran: unexpected predominance of vancomycin-resistant enterococcus faecalis. | colonization of the human and animal intestinal tract with potential pathogenic bacteria is correlated with the risk of contamination of food products. the current study analyzed the prevalence of enterococcus faecalis and escherichia coli o157h7 in ground meat in ilam, iran. both index organisms were identified following standard food microbiological methods. for e. faecalis, the susceptibility to vancomycin was tested, and pcr was used to check for the vana gene. e. faecalis was present in all ... | 2015 | 26213680 |
| antimicrobial activity of calcium hydroxide and betamethasone on enterococcus faecalis; an in vitro assessment. | calcium hydroxide (ch) is one of the most common intracanal medications. corticosteroids (cs) are used in endodontics because of their anti-inflammatory activity. this study aimed to evaluate the antimicrobial effect of ch+betamethasone and ch+saline against enterococcus faecalis (e. faecalis) using agar diffusion test and measuring the microbial zone of inhibition (zoi). | 2015 | 26213541 |
| antibacterial efficacy of pastes against e faecalis in primary root dentin: a confocal microscope study. | management of abscessed primary teeth often present endodontic failure owing to questioned efficiency of dressings or obturating pastes to eliminate enterococcus faecalis, a resistant bacterium, residing in depth of dentinal tubules. the present study evaluates the antimicrobial efficacy of two antibacterial and two obturating pastes in dentinal tubules of primary teeth infected with enterococcus faecalis using viability stain and confocal laser scanning microscope (clsm). | 2015 | 26208070 |
| genome sequences of copper resistant and sensitive enterococcus faecalis strains isolated from copper-fed pigs in denmark. | six strains of enterococcus faecalis (s1, s12, s17, s18, s19 and s32) were isolated from copper fed pigs in denmark. these gram-positive bacteria within the genus enterococcus are able to survive a variety of physical and chemical challenges by the acquisition of diverse genetic elements. the genome of strains s1, s12, s17, s18, s19 and s32 contained 2,615, 2,769, 2,625, 2,804, 2,853 and 2,935 protein-coding genes, with 41, 42, 27, 42, 32 and 44 genes encoding antibiotic and metal resistance, re ... | 2015 | 26203344 |
| antibacterial and anti-inflammatory activities of an extract, fractions, and compounds isolated from gochnatia pulchra aerial parts. | this paper reports on the in vitro antibacterial and in vivo anti-inflammatory properties of a hydroethanolic extract of the aerial parts of gochnatia pulchra (hegp). it also describes the antibacterial activity of hegp fractions and of the isolated compounds genkwanin, scutellarin, apigenin, and 3,5-o-dicaffeoylquinic acid, as evaluated by a broth microdilution method. while hegp and its fractions did not provide promising results, the isolated compounds exhibited pronounced antibacterial activ ... | 2015 | 26200228 |
| structures of folt in substrate-bound and substrate-released conformations reveal a gating mechanism for ecf transporters. | energy-coupling factor (ecf) transporters are a new family of abc transporters that consist of four subunits, two cytoplasmic atpases ecfa and ecfa' and two transmembrane proteins namely ecfs for substrate-specific binding and ecft for energy coupling. here, we report the 3.2-å resolution crystal structure of the ecfs protein of a folate ecf transporter from enterococcus faecalis-effolt, a close homologue of folt from lactobacillus brevis-lbfolt. structural and biochemical analyses reveal the re ... | 2015 | 26198469 |
| anti-inflammatory, antioxidant and antimicrobial activity characterization and toxicity studies of flowers of "jarilla", a medicinal shrub from argentina. | zuccagnia punctata cav. (fabaceae) is an argentine medicinal aromatic shrub (jarilla pispito, puspus, lata and jarilla macho). the chalcones were identified as pigments responsible for the yellow color of the flowers. hydroethanolic extracts were obtained both from fresh flowers and from flowers dried by lyophilization. the extracts were standardized by their phenolic and flavonoids content. their fingerprints by hplc-dad indicated the presence of two chalcones as major compounds (2',4'-dihydrox ... | 2015 | 26197533 |
| protective effect of enterococcus faecalis dapto 512 on the intestinal tract and gut mucosa: milk allergy application. | the allergenicity of β-lactoglobulin (β-lg) was studied by using ussing chamber in a murine model of β-lg allergy supplemented with hydrolysates obtained after fermentation of milk for 48 h at 37 (°)c with enterococcus faecalis dapto 512, isolated from cow milk and identified by 16s rdna sequence analysis. balb/c mice were sensitised intraperitoneally with β-lg. three groups of mice were formed: group 1, composed of naive mice used as control received only nacl; group 2, positive control compose ... | 2015 | 26192744 |
| repeated exposures to blue light-activated eosin y enhance inactivation of e. faecalis biofilms, in vitro. | in dentistry, antibacterial photodynamic therapy (a-pdt) has shown promising results for inactivating bacterial biofilms causing carious, endodontic and periodontal diseases. in the current study, we assessed the ability of eosin y exposed to 3 irradiation protocols at inactivating enterococcus faecalis biofilms, in vitro. | 2015 | 26188278 |
| synthesis and evaluation of isatin-β-thiosemicarbazones as novel agents against antibiotic-resistant gram-positive bacterial species. | methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus (vre) have caused an increasing mortality rate, which means that antibiotic resistance is becoming an important health issue. in the course to screen new agents for resistant bacteria, we identified that a series of isatin-β-thiosemicarbazones (ibts) could inhibit the growth of mrsa and vre. this was the first time that the "familiar" ibt compounds exhibited significant anti gram-positive pathogen activity. ... | 2015 | 26185006 |
| antimicrobial resistance and virulence traits in enterococcus strains isolated from dogs and cats. | we investigated presence and prevalence of antibiotic-resistances and other biological characters in enterococci isolated from faeces of healthy dogs and cats because these microorganisms represent important human and veterinary pathogens/opportunists, and a significant burden for healthcare systems. in all samples (n=115) we detected enterococci, with a predominance of enterococcus faecium (42; 36.5%) and enterococcus faecalis (36; 31.3%) species, endowed with virulence traits and multidrug-res ... | 2015 | 26147147 |
| estradiol protects female rats against sepsis induced by enterococcus faecalis improving leukocyte bactericidal activity. | enterococcus faecalis is a gram-positive bacteria described as an important causative agent of sepsis. the contact between host leukocytes and bacteria activates the innate immunity, participating as the first defense mechanism against infection. pro-inflammatory cytokines [including tumor necrosis factor (tnf)-α and interleukin-1β] and nitric oxide (no) are essential to recruitment of leukocytes into the infectious focus as well as their activation for phagocytosis. beyond the bacteria species, ... | 2015 | 26143494 |
| [microbiological characteristics and patterns of resistance in prosthetic joint infections in a referral hospital]. | the prosthetic joint infection is the most feared and catastrophic complication for cause severe physical damage to patients and, generates high economic costs. | 2015 | 26143043 |
| enterococcus faecalis inhibits osteoblast differentiation and induces chemokine expression. | enterococcus faecalis is commonly found in root canals of patients with refractory apical periodontitis, often accompanying inflammation and malfunctioning bone regeneration. in this study, we investigated the effect of e. faecalis on osteoblast differentiation and the ability to induce chemokine expression to recruit inflammatory cells. | 2015 | 26141768 |
| enterococcus faecalis promotes osteoclastogenesis and semaphorin 4d expression. | enterococcus faecalis is considered a major bacterial pathogen implicated in endodontic infections and contributes considerably to periapical periodontitis. this study aimed to investigate the potential mechanisms by which e. faecalis accounts for the bone destruction in periapical periodontitis in vitro. osteoclast precursor raw264.7 cells were treated with e. faecalis atcc 29212 and a wild strain of e. faecalis derived clinically from an infected root canal. the results showed that, to some ex ... | 2015 | 26138525 |
| antibacterial activity of chlorhexidine after final irrigation with ethanol: clsm and culture-based method analysis. | this study investigated the effect of 95% ethanol on the antibacterial properties of 2% chlorexidine (chx) over monospecies biofilm (enterococcus faecalis) through a culture-based method, and over multispecies biofilm using confocal laser scanning microscopy (clsm). for monospecies model, e. faecalis biofilm was induced in 40 root canals. the irrigation procedures were: s-saline solution; s/chx-saline solution + chx; e-ethanol; and e/chx-ethanol + chx. microbial sampling was performed at three p ... | 2015 | 26138134 |
| antibacterial monoterpenoid indole alkaloids from alstonia scholaris cultivated in temperate zone. | three new monoterpenoid indole alkaloids, named normavacurine-21-one (1), 5-hydroxy-19, 20-e-alschomine (2), and 5-hydroxy-19, 20-z-alschomine (3), together with thirteen known indole alkaloids (4-16) were isolated from the leaves of alstonia scholaris cultivated in kunming. their structures were elucidated on the basis of extensive spectroscopic analysis, as well as by comparison with the reported spectroscopic data. the leaves of a. scholaris cultivated in kunming, contained picrinine-type alk ... | 2015 | 26136061 |
| molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in belgrade, serbia. | in this study the distribution of species and antimicrobial resistance among vancomycin resistant enterococci (vre) recovered from clinical specimens obtained from five hospitals in belgrade was analyzed. strains were further characterized by pulsed-field gel electrophoresis (pfge). polymerase chain reaction (pcr) was used to investigate the presence of vana and vanb genes and pathogenicity factor genes. identification of 194 vre isolates revealed 154 enterococcus faecium, 21 enterococcus faecal ... | 2015 | 26132835 |
| establishment and characterization of uti and cauti in a mouse model. | urinary tract infections (uti) are highly prevalent, a significant cause of morbidity and are increasingly resistant to treatment with antibiotics. females are disproportionately afflicted by uti: 50% of all women will have a uti in their lifetime. additionally, 20-40% of these women who have an initial uti will suffer a recurrence with some suffering frequent recurrences with serious deterioration in the quality of life, pain and discomfort, disruption of daily activities, increased healthcare ... | 2015 | 26132341 |
| clinical features and treatment of endophthalmitis after cataract surgery. | the aim of this study was to investigate the clinical features and treatment results of endophthalmitis after cataract surgery. five patients with endophthalmitis after phacoemulsification with intraocular lens implantation were enrolled in this study. the pathogenesis, clinical manifestation, and surgical outcomes of 5 patients were compared. three patients were surgically treated with anterior chamber irrigation and vitrectomy with intravitreal injection. the remaining two patients were medica ... | 2015 | 26125869 |
| evaluation of an indigenously prepared herbal extract (endopam) as an antimicrobial endodontic irrigant: an ex vivo study. | root canal irrigation plays a pivotal role in endodontics. constant increase in antibiotic resistance and side effects caused by synthetic irrigants has shifted the research toward developing herbal alternatives. the current study aims to assess the ex vivo effectiveness of an indigenously prepared herbal extract "endopam" and compare it with the conventional endodontic irrigants for disinfection of root canals infected with enterococcus faecalis. | 2015 | 26124607 |
| from (p)ppgpp to (pp)pgpp: characterization of regulatory effects of pgpp synthesized by the small alarmone synthetase of enterococcus faecalis. | the bacterial stringent response (sr) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. this response is mediated by the intracellular accumulation of the alarmones pppgpp and ppgpp, collectively called (p)ppgpp. in enterococcus faecalis, (p)ppgpp metabolism is carried out by the bifunctional synthetase/hydrolase e. faecalis rel (relef) and the small alarmone synthetase (sas) relqef. although rel is the main enzyme responsible for sr ... | 2015 | 26124242 |
| codon-optimized fluorescent mtfp and mcherry for microscopic visualization and genetic counterselection of streptococci and enterococci. | despite the powerful potential of fluorescent proteins for labeling bacteria, their use has been limited in multi-species oral biofilm models. fermentative metabolism by streptococcal species that initiate biofilm colonization results in an acidic, reduced microenvironment that may limit the activities of some fluorescent proteins which are influenced by ph and oxygen availability. the need to reliably distinguish morphologically similar strains within biofilms was the impetus for this work. tea ... | 2015 | 26122309 |
| a novel rat model of catheter-associated urinary tract infection. | the authors aimed to establish a rat model of catheter-associated utis using a complete urethral catheter. bacterial growth in biofilms on urethral catheters was analyzed using standard culture methods to validate this model. | 2015 | 26122120 |
| enterococcal endocarditis revisited. | the enterococcus species is the third main cause of infective endocarditis (ie) worldwide, and it is gaining relevance, especially among healthcare-associated cases. patients with enterococcal ie are older and have more comorbidities than other types of ie. classical treatment options are limited due to the emergence of high-level aminoglycosides resistance (hlar), vancomycin resistance and multidrug resistance in some cases. besides, few new antimicrobial alternatives have shown real efficacy, ... | 2015 | 26118390 |
| nanostructured composite material graphite/tio2 and its antibacterial activity under visible light irradiation. | the paper addresses laboratory preparation, characterization and in vitro evaluation of antibacterial activity of graphite/tio2 nanocomposites. composites graphite/tio2 with various ratio of tio2 nanoparticles (30wt.%, and 50wt.%) to graphite were prepared using a thermal hydrolysis of titanylsulfate in the presence of graphite particles, and subsequently dried at 80°c. x-ray powder diffraction, transmission electron microscopy and raman microspectroscopy served as phase-analytical methods disti ... | 2015 | 26114221 |
| the natural surfactant glycerol monolaurate significantly reduces development of staphylococcus aureus and enterococcus faecalis biofilms. | bacterial biofilms are involved in a large proportion of clinical infections, including device-related infections. unfortunately, biofilm-associated bacteria are typically less susceptible to antibiotics, and infected devices must often be removed. on the basis of a recent observation that lipid-rich biofilm matrix material is present in early biofilm formation and may protect a population of bacteria from interacting with ordinarily diffusible small molecules, we hypothesized that surfactants m ... | 2015 | 26110557 |
| [diagnosis in patients with a painful arthroplasty]. | the differential diagnosis between asepting loosening or prosthetic joint infection is not always easy. tc-99m-labelled leucocyte scans, frozen section and histology can help recognise doubtful cases. we report the experience of the unit for infectious diseases and septic orthopaedics of the asl-2 liguria, italy, with a tc-99m-labelled leucocyte scan and intraoperative frozen section to choose the best therapeutic approach: one-stage or two-stage exchange or arthrodesis-arthroplastica. all cases ... | 2015 | 26110294 |
| characterization of lactobacillus fermentum pl9988 isolated from healthy elderly korean in a longevity village. | in this work, we wanted to develop a probiotic from famous longevity villages in korea. we visited eight longevity villages in korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. isolated colonies that appeared on mrs agar containing bromophenol blue were identified by means of 16s rrna sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 ... | 2015 | 26095384 |
| combined antibacterial effect of sodium hypochlorite and root canal sealers against enterococcus faecalis biofilms in dentin canals. | the present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (naocl) and root canal sealers on enterococcus faecalis biofilms using a dentin infection model. | 2015 | 26092772 |
| sol-gel-derived bioactive glasses demonstrate antimicrobial effects on common oral bacteria. | to determine the antibacterial effect of nano-structured, sol-gel processed bioactive glasses that may be used for implants, coatings, and as adjuncts to dental restorative materials. | 2015 | 26087578 |
| preterm infant gut colonization in the neonatal icu and complete restoration 2 years later. | preterm infants in a neonatal intensive care unit (nicu) are exposed to multidrug-resistant bacteria previously adapted to the hospital environment. the aim of the present study was to characterize the bacterial antibiotic-resistant high-risk lineages colonizing preterm infants during their nicu stay and their persistence in faeces after 2 years. a total of 26 preterm neonates were recruited between october 2009 and june 2010 and provided 144 faecal samples. milk samples (86 mother's milk, 35 hu ... | 2015 | 26086569 |
| low-dose gentamicin for uncomplicated enterococcus faecalis bacteremia may be nephrotoxic in children. | uncertainty exists regarding the role of synergistic gentamicin for uncomplicated enterococcus faecalis bacteremia in children. | 2015 | 26082503 |
| phenotypic and genotypic characterization of lactic acid bacteria isolated from raw goat milk and effect of farming practices on the dominant species of lactic acid bacteria. | lactic acid bacteria, in particular lactococcus lactis, play a decisive role in the cheese making process and more particularly in lactic cheeses which are primarily produced on goat dairy farms. the objective of this study was therefore to identify the main lactic acid bacteria found in raw goats' milk from three different regions in france and evaluate if certain farming practices have an effect on the distribution of species of lactic acid bacteria in the various milk samples. identification ... | 2015 | 26082325 |
| adherence and intracellular survival within human macrophages of enterococcus faecalis isolates from coastal marine sediment. | enterococcus faecalis is part of the human intestinal microbiota and an important nosocomial pathogen. it can be found in the marine environment, where it is also employed as a fecal indicator. to assess the pathogenic potential of marine e. faecalis, four strains isolated from marine sediment were analyzed for their ability to survive in human macrophages. escherichia coli dh5α was used as a negative control. the number of adherent and intracellular bacteria was determined 2.5 h after the infec ... | 2015 | 26079735 |
| non-linear significant relationship between use of glycopeptides and isolation of vancomycin-resistant enterococcus species in a university hospital setting. | emergence of colonization and infection with vancomycin-resistant enterococci (vre) has become a worldwide challenge. to investigate whether the increasing incidence of vre isolation can be correlated with use of glycopeptides in the hospital setting, we conducted a hospital-wide two-year study in the university hospital of vienna. | 2015 | 26078865 |
| evaluation of the enterococcus faecalis biofilm-associated virulence factors ahrc and eep in rat foreign body osteomyelitis and in vitro biofilm-associated antimicrobial resistance. | enterococcus faecalis can cause healthcare-associated biofilm infections, including those of orthopedic devices. treatment of enterococcal prosthetic joint infection is difficult, in part, due to biofilm-associated antimicrobial resistance. we previously showed that the e. faecalis og1rf genes ahrc and eep are in vitro biofilm determinants and virulence factors in animal models of endocarditis and catheter-associated urinary tract infection. in this study, we evaluated the role of these genes in ... | 2015 | 26076451 |
| genetic relationships among enterococcus faecalis isolates from different sources as revealed by multilocus sequence typing. | enterococcus faecalis is part of the natural gut flora of humans and other mammals; some isolates are also used in food production. so, it is important to evaluate the genetic diversity and phylogenetic relationships among e. faecalis isolates from different sources. multilocus sequence typing protocol was used to compare 39 e. faecalis isolates from chinese traditional food products (including dairy products, acidic gruel) and 4 published e. faecalis isolates from other sources including human- ... | 2015 | 26074239 |
| rna-based assay demonstrated enterococcus faecalis metabolic activity after chemomechanical procedures. | because ribosomal rna (rrna) indicates metabolic cell activity, this study aimed to evaluate the sensitivity of rrna-based quantitative polymerase chain reaction (rt-qpcr) for the identification of active enterococcus faecalis in root canals samples compared with a method based on ribosomal dna (rdna) (rrna genes). | 2015 | 26071099 |
| surface-associated lipoproteins link enterococcus faecalis virulence to colitogenic activity in il-10-deficient mice independent of their expression levels. | the commensal enterococcus faecalis is among the most common causes of nosocomial infections. recent findings regarding increased abundance of enterococci in the intestinal microbiota of patients with inflammatory bowel diseases and induction of colitis in il-10-deficient (il-10-/-) mice put a new perspective on the contribution of e. faecalis to chronic intestinal inflammation. based on the expression of virulence-related genes in the inflammatory milieu of il-10-/- mice using rna-sequencing an ... | 2015 | 26067254 |
| rampant parasexuality evolves in a hospital pathogen during antibiotic selection. | horizontal gene transfer threatens the therapeutic success of antibiotics by facilitating the rapid dissemination of resistance alleles among bacterial species. the conjugative mobile element tn916 provides an excellent context for examining the role of adaptive parasexuality as it carries the tetracycline-resistance allele tetm and has been identified in a wide range of pathogens. we have used a combination of experimental evolution and allelic frequency measurements to gain insights into the a ... | 2015 | 26060280 |
| purification and characterization of a cysteine-rich 14-kda antibacterial peptide from the granular hemocytes of mangrove crab episesarma tetragonum and its antibiofilm activity. | antimicrobial peptide (amp) crustin is a type of immune molecule present in the immune system of crustaceans and response against microbial invasion. in the present study, we have identified and characterized the cationic, amphipathic structure consisting of amp crustin from a mangrove crab episesarma tetragonum using cm sepharose-based cation exchange column chromatography. e. tetragonum crustin showed a single band of 14 kda on sds-page and the homogeneity showed retention time of 8.4 min in r ... | 2015 | 26059067 |
| aortico-left atrial fistula: a rare complication of bioprosthetic aortic valve endocarditis secondary to enterococcus faecalis. | paravalvular aortic root abscess with intracardiac fistula formation is an exceedingly rare complication of infective endocarditis. this condition is even more rarely encountered in patients with bioprosthetic valve endocarditis. we report an unusual case of a 68-year-old bosnian female with a bioprosthetic aortic valve, who developed an extensive aortic root abscess, complicated by an aortico-left atrial intracardiac fistula. this case illustrates that a high index of suspicion, prompt diagnosi ... | 2015 | 26246917 |
| diversity and dynamics of antibiotic-resistant bacteria in cheese as determined by pcr denaturing gradient gel electrophoresis. | this work reports the composition and succession of tetracycline- and erythromycin-resistant bacterial communities in a model cheese, monitored by polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge). bacterial 16s rrna genes were examined using this technique to detect structural changes in the cheese microbiota over manufacturing and ripening. total bacterial genomic dna, used as a template, was extracted from cultivable bacteria grown without and with tetracycline or e ... | 2015 | 26241491 |
| antibiotic resistance patterns of bacteria causing urinary tract infections in the elderly living in nursing homes versus the elderly living at home: an observational study. | antibiotic resistance is a problem in nursing homes. presumed urinary tract infections (uti) are the most common infection. this study examines urine culture results from elderly patients to see if specific guidelines based on gender or whether the patient resides in a nursing home (nh) are warranted. | 2015 | 26238248 |
| additional disinfection with a modified salt solution in a root canal model. | the aim of this study is to investigate the disinfecting properties of a modified salt solution (mss) and calcium hydroxide (ca(oh)2) in a non-direct-contact ex-vivo model. | 2015 | 26234625 |
| infection control in retreatment cases: in vivo antibacterial effects of 2 instrumentation systems. | this in vivo study compared the antibacterial effects of 2 instrumentation systems in root canal-treated teeth with apical periodontitis. | 2015 | 26234543 |
| detection of identical isolates of enterococcus faecalis from the blood and oral mucosa in a patient with infective endocarditis. | the detection of infective endocarditis (ie) of oral origin has been previously discussed. however, there are few reports confirming this infection using molecular biological techniques. we herein describe the case of a 67-year-old man who developed ie. blood culture samples and strains obtained from the gingival and buccal mucosa showed 100% identity to enterococcus faecalis jcm 5803 on sequencing of 16s rrna gene fragments. a random amplification of polymorphic dna (rapd) analysis showed the s ... | 2015 | 26179542 |
| plasma deposition of silver nanoparticles on ultrafiltration membranes: antibacterial and anti-biofouling properties. | a novel and versatile plasma reactor was used to modify polyethersulphone commercial membranes. the equipment was applied to: i) functionalize the membranes with low-temperature plasmas, ii) deposit a film of poly(methyl methacrylate) (pmma) by plasma enhanced chemical vapor deposition (pecvd) and, iii) deposit silver nanoparticles (snp) by gas flow sputtering. each modification process was performed in the same reactor consecutively, without exposure of the membranes to atmospheric air. scannin ... | 2015 | 26166926 |
| japanese nationwide surveillance in 2011 of antibacterial susceptibility patterns of clinical isolates from complicated urinary tract infection cases. | to investigate antimicrobial susceptibility patterns of various bacterial pathogens isolated from complicated urinary tract infection (uti) cases, the japanese society of chemotherapy, the japanese association of infectious disease, and the japanese society of clinical microbiology conducted the second nationwide surveillance from january to september 2011. with the cooperation of 42 medical institutions throughout japan, 1036 strains belonging to 8 clinically relevant bacterial species were col ... | 2015 | 26166322 |
| the bacterial spectrum and antimicrobial susceptibility in female recurrent urinary tract infection: how different they are from sporadic single episodes? | to analyze and compare the etiological uropathogens and the susceptibility profile findings on urine culture between sporadic cases of community-acquired, uncomplicated urinary tract infection (uti) and recurrent uti cases in women. | 2015 | 26163810 |
| an in vitro comparison of endodontic medicaments propolis and calcium hydroxide alone and in combination with ciprofloxacin and moxifloxacin against enterococcus faecalis. | to evaluate and compare the antimicrobial properties of propolis and calcium hydroxide alone and in combination with ciprofloxacin and moxifloxacin against enterococcus faecalis (e. faecalis). | 2015 | 26162260 |
| antibacterial potential of 2.5% sodium hypochlorite in distinct irrigation protocols on enterococcus faecalis biofilm. | the aim of this study was to evaluate the effect of irrigation methods on antibacterial potential of 2.5% naocl on enterococcus faecalis biofilm. | 2015 | 26162251 |
| spread of enterococcal surface protein in antibiotic resistant entero-coccus faecium and enterococcus faecalis isolates from urinary tract infections. | enterococci rank among leading cause of nosocomial bacteremia and urinary tract infection in hospital and community acquired infections. several traits that may contribute to enhanced virulence have been identified in enterococci. extracellular surface protein (esp) is a virulence factor that contributes in biofilm formation and resistance to environmental stresses. in this study we aimed to determine occurrence of esp in e. faecium and e. faecalis isolates isolated from urinary tract infections ... | 2015 | 26161154 |
| antimicrobial activity of the essential oil of plectranthus neochilus against cariogenic bacteria. | this work used the broth microdilution method to investigate the antimicrobial activity of the essential oil obtained from the leaves of plectranthus neochilus (pn-eo) against a representative panel of oral pathogens. we assessed the antimicrobial activity of this oil in terms of the minimum inhibitory concentration (mic). pn-eo displayed moderate activity against enterococcus faecalis (mic = 250 μg/ml) and streptococcus salivarus (mic = 250 μg/ml), significant activity against streptococcus sob ... | 2015 | 26161115 |
| adaptation to nacl reduces the susceptibility of enterococcus faecalis to melaleuca alternifolia (tea tree) oil. | this study investigated the hypothesis that the salt adaptation response of enterococcus faecalis alters susceptibility to tea tree oil (tto). six e. faecalis isolates were adapted to 6.5 % nacl, and then exposed to tto in phosphate-buffered saline (pbs). one isolate was also exposed to tto in brain heart infusion broth (bhib). the viability of salt-adapted and non-adapted control cells was determined at 0, 45 and 90 min and compared. mics for several antibiotics and tto were also determined by ... | 2015 | 26159776 |
| antibiotic selective pressure and development of bacterial resistance detected in bacteriuria following kidney transplantation. | bacteriuria (symptomatic and asymptomatic) is the most common infectious complication after kidney transplantation. this study aimed to determine its prevalence among kidney transplant recipients hospitalized after transplantation, respective risk factors, and frequency of isolates and antibacterial susceptibility. | 2015 | 26036537 |
| surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic escherichia coli biofilms against pathogen colonization. | prevention of pathogenic colonization on medical devices over a long period of time remains a great challenge, especially in a high-nutrient environment that accelerates production of biomass leading to biofouling of the device. since biofouling and the subsequent pathogen colonization is eventually inevitable, a new strategy using non-pathogenic bacteria as living guards against pathogenic colonization on medical devices has attracted increasing interest. crucial to the success of this strategy ... | 2015 | 26029359 |
| evaluation of cold plasma treatment and safety in disinfecting 3-week root canal enterococcus faecalis biofilm in vitro. | although endodontic infection is caused by multi-bacteria species, enterococcus faecalis is usually isolated in chronic apical periodontitis. the aim of this study was to evaluate the effectiveness and mechanical safety of cold plasma therapy in disinfecting 3-week e. faecalis biofilms. | 2015 | 26027875 |
| susceptibility patterns of uropathogens identified in hospitalised children with community-acquired urinary tract infections in thrace, greece. | urinary tract infections (utis) are common infections associated with considerable morbidity and mortality, particularly in paediatric patients. the alarmingly increasing antimicrobial resistance of contemporary uropathogens in children necessitates the re-evaluation of antibiotic treatment. we evaluated uropathogens isolated from children hospitalised due to a community-acquired uti over a 5.5-year period in a university hospital of northern greece and their antibiotic susceptibility patterns. ... | 2015 | 27873675 |
| evaluation of the effect of enterococcus faecalis biofilm on the 2% chlorhexidine substantivity: an in vitro study. | the aim of this study was to correlate the bacterial viability and the presence of 2% chlorhexidine (chx) solution on dentin by means of confocal laser scanning microscopy and high-performance liquid chromatography for 48 hours, 7 days, and 30 days. | 2015 | 26025346 |
| statins and antimicrobial effects: simvastatin as a potential drug against staphylococcus aureus biofilm. | statins are important lipid-lowering agents with other pleiotropic effects. several studies have explored a possible protective effect of statins to reduce the morbidity and mortality of many infectious diseases. staphylococcus aureus is one of the main pathogens implicated in nosocomial infections; its ability to form biofilms makes treatment difficult. the present study observed the mic of atorvastatin, pravastatin and simvastatin against s. aureus, pseudomonas aeruginosa, escherichia coli and ... | 2015 | 26020797 |
| analysis of vancomycin susceptibility testing results for presumptive categorization of telavancin. | scattergrams between vancomycin and telavancin demonstrated susceptibility agreement rates of 99.96, 99.65, and 100.00% for staphylococcus aureus, enterococcus faecalis, and streptococci, respectively. a single very major error was obtained against e. faecalis, while vancomycin-intermediate s. aureus (visa) and vancomycin-resistant and teicoplanin-susceptible (vanb phenotype) e. faecalis were responsible for major and minor errors. these results support the use of vancomycin to infer telavancin ... | 2015 | 26019194 |
| a novel termini analysis theory using hts data alone for the identification of enterococcus phage ef4-like genome termini. | enterococcus faecalis and enterococcus faecium are typical enterococcal bacterial pathogens. antibiotic resistance means that the identification of novel e. faecalis and e. faecium phages against antibiotic-resistant enterococcus have an important impact on public health. in this study, the e. faecalis phage ime-ef4, e. faecium phage ime-efm1, and both their hosts were antibiotic resistant. to characterize the genome termini of these two phages, a termini analysis theory was developed to provide ... | 2015 | 26016565 |
| the enterococcus faecalis ebpa pilus protein: attenuation of expression, biofilm formation, and adherence to fibrinogen start with the rare initiation codon att. | the endocarditis and biofilm-associated pili (ebp) are important in enterococcus faecalis pathogenesis, and the pilus tip, ebpa, has been shown to play a major role in pilus biogenesis, biofilm formation, and experimental infections. based on in silico analyses, we previously predicted that att is the ebpa translational start codon, not the atg codon, 120 bp downstream of att, which is annotated as the translational start. att is rarely used to initiate protein synthesis, leading to our hypothes ... | 2015 | 26015496 |
| correlation between enterococcus faecalis and persistent intraradicular infection compared with primary intraradicular infection: a systematic review. | the relationship between enterococcus faecalis and pulpal or periradicular diseases has been studied for many years; however, whether e. faecalis is correlated with persistent intraradicular infections (teeth after failed endodontic treatments) compared with primary intraradicular infections remains controversial. the objective of this systematic review was to compare the prevalence of e. faecalis in primary and persistent intraradicular infections. | 2015 | 26015157 |
| uses and limitations of green fluorescent protein as a viability marker in enterococcus faecalis: an observational investigation. | enterococci are capable of producing biofilms that are notoriously difficult to treat and remove, for instance in root canal infections. the tenacious nature of these organisms makes screening of known and novel antimicrobial compounds necessary. while traditionally growth and fluorescence-based screening methods have proven useful, these methods have their limitations when applied to enterococci (e.g. time consuming, no kinetic data, diffusion properties of the fluorescent dyes). the aim of thi ... | 2015 | 26015063 |
| expression of interleukin-1β and matrix metalloproteinase-8 in cytolytic and noncytolytic enterococcus faecalis-induced persistent apical periodontitis: a comparative study in the rat. | cytolytic enterococcus faecalis possesses a highly toxic and proinflammatory capacity. cytokines and proteases play important roles in the host inflammatory response. the aim of this study was to compare the local expression of interleukin (il)-1β and matrix metalloproteinase-8 (mmp-8) between persistent apical periodontitis (ap) infected by cytolytic and noncytolytic e. faecalis. | 2015 | 26055433 |
| blocking the 'midas' touch of enterococcus faecalis. | 2015 | 26046076 | |
| effects of fixed and removable space maintainers on plaque accumulation, periodontal health, candidal and enterococcus faecalis carriage. | to evaluate the effects of space maintainers on plaque accumulation, periodontal health and oral microflora. | 2015 | 26044443 |
| pheromone killing of multidrug-resistant enterococcus faecalis v583 by native commensal strains. | multidrug-resistant enterococcus faecalis possess numerous mobile elements that encode virulence and antibiotic resistance traits as well as new metabolic pathways, often constituting over one-quarter of the genome. it was of interest to determine how this large accretion of mobile elements affects competitive growth in the gastrointestinal (gi) tract consortium. we unexpectedly observed that the prototype clinical isolate strain v583 was actively killed by gi tract flora, whereas commensal ente ... | 2015 | 26039987 |
| identification of enterococcus faecalis and pseudomonas aeruginosa on and in implants in individuals with peri-implant disease: a cross-sectional study. | to verify whether the parts of dental implants can be contaminated by opportunistic pathogens. | 2015 | 26009909 |
| synthesis, structural characterization and biological activity of novel cyclohexane-1,3-dione ligands and their metal complexes. | some new zn(ii) and cu(ii) complexes [cu(l1)(oac)2]∙h2o, [cu(l1)(no3)h2o]∙no3∙3.5h2o, [zn(l1)(no3)2]∙4.5h2o, [zn(l1)(oac)2(h2o)2]∙3h2o, [cu2(l2)(oac)4]∙2h2o∙2dmf, [cu(l2)2]∙2no3∙1.5dmf∙h2o, [zn(l2)2(no3)2]∙dmf and [zn2(l2)(oac)4(h2o)4]∙5h2o; l1 = 2-[2-(2-methoxyphenyl)hydrazono]cyclohexane-1,3-dione and l2 = 2-[2-(3-nitrophenyl)hydrazono]cyclohexane-1,3-dione were synthesized and characterized by ir, 1h-nmr,13c-nmr and ultraviolet (uv-vis.) spectroscopy, elemental analysis, magnetic susceptibili ... | 2015 | 26007185 |
| the mazef toxin-antitoxin system as an attractive target in clinical isolates of enterococcus faecium and enterococcus faecalis. | the toxin-antitoxin (ta) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. in this study, the prevalence of ta systems in independently isolated clinical isolates of enterococcus faecium and enterococcus faecalis was determined, the dominant ta system was identified, different virulence genes in e. faecium and e. faecalis were surveyed, the level of expression of the virulence and ta genes in normal and stress conditions was determined, and f ... | 2015 | 26005332 |
| overexpression of enterococcus faecalis elr operon protects from phagocytosis. | mechanisms underlying the transition from commensalism to virulence in enterococcus faecalis are not fully understood. we previously identified the enterococcal leucine-rich protein a (elra) as a virulence factor of e. faecalis. the elra gene is part of an operon that comprises four other orfs encoding putative surface proteins of unknown function. | 2015 | 26003173 |
| probiotic features of lactobacillus strains isolated from ragusano and pecorino siciliano cheeses. | in the present study 177 lactobacillus spp. strains, isolated from ragusano and pecorino siciliano cheeses, were in vitro screened for probiotic traits, and their characteristics were compared to those of lactobacillus rhamnosus gg, commercial strain. based on acidic and bile salt resistance, thirteen lactobacillus strains were selected. the multiplex-pcr application revealed that nine strains belonged to l. rhamnosus species and four to lactobacillus paracasei species. all selected strains were ... | 2015 | 25998823 |
| mckittrick-wheelock syndrome complicated by enterococcus faecalis endocarditis: a unique combination. | 2015 | 25112151 | |
| mutational analysis of critical residues of fad-independent catabolic acetolactate synthase from enterococcus faecalis v583. | catabolic acetolactate synthase (cals) from enterococcus faecalis is a fad-independent enzyme, which catalyzes the condensation of two molecules of pyruvate to produce acetolactate. mutational and kinetic analyses of variants suggested the importance of h111, q112, and q411 residues for catalysis in cals. the wild-type and variants were expressed as equally soluble proteins and co-migrated to a size of 60 kda on sds-page. importantly, h111 in cals, which is widely present as phenylalanine in man ... | 2015 | 25128823 |
| relationships between the antibacterial activity of sodium hypochlorite and treatment time and biofilm age in early enterococcus faecalis biofilms. | to determine the relationships between the antibacterial activity of naocl and treatment time and biofilm age in early enterococcus faecalis biofilms using a linear fitting procedure. | 2015 | 25156394 |
| recurrent linezolid-resistant enterococcus faecalis infection in a patient with pneumonia. | it has been reported that lzd-resistant enterococcus in the gastrointestinal tract of mice colonizes persistently and shows variable minimum inhibitor concentration (mic) values. however, the colonization characteristics of enterococcus with lzd resistance in patients remain elusive. here, we report the case of a patient with recurrent pneumonia due to infection with lzd-resistant enterococcus faecalis strains. the colonization characteristics of the strains isolated from this patient were analy ... | 2015 | 25236388 |
| construction of a chimeric lysin ply187n-v12c with extended lytic activity against staphylococci and streptococci. | developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. in the present work, a novel chimeric lysin (ply187n-v12c) was constructed by fusing the catalytic domain (ply187n) of the bacteriophage lysin ply187 with the cell binding domain (146-314aa, v12c) of the lysin plyv12. the results showed that the chimeric lysin ply187n-v12c had not only lytic activity similar to ply187n against staphylococcal strains but al ... | 2015 | 25219798 |
| oligomerization of esculin improves its antibacterial activity and modulates antibiotic resistance. | in this particular study, the antibacterial activity of esculin and oligomer fractions was assessed. mic values of esculin and its oligomer fractions as well as of some antibiotics against gram-positive and gram-negative strains and against escherichia coli multiresistant variants were determined by the standard broth microdilution method. both esculin and oligoesculin fractions exhibited antibacterial effect against reference strains; staphylococcus aureus, enterococcus faecalis, salmonella ent ... | 2015 | 25248728 |
| transcriptomic response of enterococcus faecalis v583 to low hydrogen peroxide levels. | enterococcus faecalis is a gram-positive commensal bacterium inhabiting the gastrointestinal tracts of human and other mammals, but is also increasingly recognized as an opportunistic human pathogen. oxidative stress is one of the major challenges encountered by enterococci, both in their natural environment and during infection. in this paper, we evaluated the transcriptomic response of e. faecalis to oxidative stress, and showed that transcript abundance was reduced for 93 genes and increased ... | 2015 | 25245959 |
| positively charged imidazolium-based ionic liquid-protected silver nanoparticles: a promising disinfectant in root canal treatment. | to synthesize and characterize silver nanoparticles (ag nps) with different surface charges in order to evaluate their cytotoxicity and antibacterial activity in the absence and presence of dentine compared with naocl and chx. | 2015 | 25269666 |
| ceftobiprole and ampicillin increase daptomycin susceptibility of daptomycin-susceptible and -resistant vre. | the synergistic combination of daptomycin plus ampicillin has proven to be effective against vre including daptomycin-non-susceptible strains. ceftobiprole is a cephalosporin with broad binding affinity for enterococcal pbp subtypes including pbp5. given the synergy between β-lactams and daptomycin against vre, it was of interest to determine whether ceftobiprole offered any synergistic advantage with daptomycin compared with ampicillin. | 2015 | 25304643 |
| the type i toxin-antitoxin par locus from enterococcus faecalis plasmid pad1: rna regulation by both cis- and trans-acting elements. | the pad1 par determinant was the first type i toxin-antitoxin system identified in gram-positive bacteria and has recently been shown to be the prototype of a family of loci that is widespread in these organisms. all family members have (i) convergently transcribed toxin message and regulatory rnas, (ii) three non-contiguous complementary regions for potential interaction, and (iii) intramolecular structures within the toxin message that modulate translation and transcript stability. therefore, ... | 2015 | 25312777 |
| distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using rna- and dna-based quantitative pcr assays. | the identification of fecal pollution sources is commonly carried out using dna-based methods. however, there is evidence that dna can be associated with dead cells or present as "naked dna" in the environment. furthermore, it has been shown that rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) assays can be more sensitive than rrna gene-based qpcr assays since metabolically active cells usually contain higher numbers of ribosomes than quiescent cells. to this end, we compared the ... | 2015 | 25326295 |
| oxidative stress enhances cephalosporin resistance of enterococcus faecalis through activation of a two-component signaling system. | enterococcus faecalis is a low-gc gram-positive bacterium, a normal resident of the gastrointestinal (gi) tract, and an important hospital-acquired pathogen. an important risk factor for hospital-acquired enterococcal infections is prior therapy with broad-spectrum cephalosporins, antibiotics that impair cell wall biosynthesis by inhibiting peptidoglycan cross-linking. enterococci are intrinsically resistant to cephalosporins; however, environmental factors that modulate cephalosporin resistance ... | 2015 | 25331701 |
| baseline activity of telavancin against gram-positive clinical isolates responsible for documented infections in u.s. hospitals (2011-2012) as determined by the revised susceptibility testing method. | telavancin had mic50 and mic90 values of 0.03 and 0.06 μg/ml (100.0% susceptible), respectively, against methicillin-resistant and -susceptible staphylococcus aureus. telavancin was active against vancomycin-susceptible enterococcus faecalis (mic50/90, 0.12/0.12 μg/ml; 100% susceptible) and enterococcus faecium (mic50/90, 0.03/0.06 μg/ml), while higher mic values were obtained against vancomycin-resistant e. faecium (mic50/90, 1/2 μg/ml) and e. faecalis (mic50/90, >2/>2 μg/ml). streptococci show ... | 2015 | 25348529 |
| activity of oritavancin against gram-positive clinical isolates responsible for documented skin and soft-tissue infections in european and us hospitals (2010-13). | to assess oritavancin activity in vitro against clinically relevant gram-positive pathogens causing skin and soft-tissue infections (sstis) in european and us hospitals. | 2015 | 25362568 |
| a liar deletion restores susceptibility to daptomycin and antimicrobial peptides in multidrug-resistant enterococcus faecalis. | daptomycin is a lipopeptide antibiotic that is used clinically against many gram-positive bacterial pathogens and is considered a key frontline bactericidal antibiotic to treat multidrug-resistant enterococci. emergence of daptomycin resistance during therapy of serious enterococcal infections is a major clinical issue. in this work, we show that deletion of the gene encoding the response regulator, liar (a member of the liafsr system that controls cell envelope homeostasis), from daptomycin-res ... | 2015 | 25362197 |
| genomic annotation for the temperate phage efc-1, isolated from enterococcus faecalis kbl101. | the temperate phage efc-1 was newly isolated from a mitomycin-c-induced lysate of enterococcus faecalis kbl101. efc-1 has an isometric head and a long tail. the phage belongs to the family siphoviridae according to its genomic structure and morphology. the phage efc-1 has 40,286 base pairs of double-stranded dna and a g+c content of 35.05 %. bioinformatic analysis of the phage revealed 60 putative open reading frames (orfs). the genome of the temperate phage efc-1 was not significantly similar t ... | 2015 | 25359106 |
| bacteriocin protein bacl1 of enterococcus faecalis targets cell division loci and specifically recognizes l-ala2-cross-bridged peptidoglycan. | bacteriocin 41 (bac41) is produced from clinical isolates of enterococcus faecalis and consists of two extracellular proteins, bacl1 and baca. we previously reported that bacl1 protein (595 amino acids, 64.5 kda) is a bacteriolytic peptidoglycan d-isoglutamyl-l-lysine endopeptidase that induces cell lysis of e. faecalis when an accessory factor, baca, is copresent. however, the target of bacl1 remains unknown. in this study, we investigated the targeting specificity of bacl1. fluorescence micros ... | 2015 | 25368300 |
| estimation of cultivable bacterial diversity in the cloacae and pharynx in eurasian griffon vultures (gyps fulvus). | in this work, we describe the biodiversity of cloacal and pharynx culture-based bacteria (commensal and pathogenic), in 75 eurasian griffon vultures (gyps fulvus) from two geographic areas. we address the question of whether the cultivable microbiota of vultures is organised into assemblages occurring by chance. in addition, we assess bacterial diversity in both anatomic regions and geographic areas. bacterial diversity was represented by 26 gram-negative and 20 gram-positive genera. the most co ... | 2015 | 25388757 |