Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the type i toxin-antitoxin par locus from enterococcus faecalis plasmid pad1: rna regulation by both cis- and trans-acting elements. | the pad1 par determinant was the first type i toxin-antitoxin system identified in gram-positive bacteria and has recently been shown to be the prototype of a family of loci that is widespread in these organisms. all family members have (i) convergently transcribed toxin message and regulatory rnas, (ii) three non-contiguous complementary regions for potential interaction, and (iii) intramolecular structures within the toxin message that modulate translation and transcript stability. therefore, ... | 2015 | 25312777 |
| photodynamic killing of enterococcus faecalis in dentinal tubules using mthpc incorporated in liposomes and invasomes. | the present in vitro study investigates the antimicrobial photodynamic efficiency of the photosensitizer 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (mthpc) incorporated in liposomes (lip) and highly flexible invasomes (inv) on the endodontopathogenic species enterococcus faecalis in infected dental root canals. | 2015 | 24950607 |
| autoimmune neutropenia of infancy with recurrent urinary tract infections: a case report. | autoimmune neutropenia of infancy is characterized by minor intercurrent infections despite severe neutropenia; severe bacterial infections are uncommon. an infant developed recurrent urinary tract infections at 9 and 11 months of age. the identified uropathogens were escherichia coli and enterococcus faecalis, respectively. empirical treatment with carbapenems, as broad-spectrum antibiotics, promptly resolved the infection without sequelae. febrile neutropenic children with cancer and autoimmun ... | 2015 | 24942028 |
| potential for nosocomial transmission of enterococcus faecalis from surfaces in dental operatories. | to assess the potential for nosocomial transmission of enterococcus faecalis during root canal treatment by measuring its occurrence on surfaces in dental operatories in relation to the efficacy of disinfection routines. | 2015 | 25066305 |
| commensal bacteria drive endogenous transformation and tumour stem cell marker expression through a bystander effect. | commensal bacteria and innate immunity play a major role in the development of colorectal cancer (crc). we propose that selected commensals polarise colon macrophages to produce endogenous mutagens that initiate chromosomal instability (cin), lead to expression of progenitor and tumour stem cell markers, and drive crc through a bystander effect. | 2015 | 24906974 |
| in vitro efficacy of antibiotic beads in treating abdominal vascular graft infections. | abdominal aortic vascular graft infection often involves several different organisms. antibiotic polymethyl methacrylate (pmma) beads may be effective in controlling infection after débridement, but bacterial species identification and antibiotic susceptibility are often not available at the time of operation, generating a need for a broad-spectrum drug combination for empirical use. we sought to determine an effective antibiotic in pmma beads for use in abdominal vascular graft infection. | 2015 | 24745942 |
| urinary tract infection caused by enterococcus isolates: aetiology and antimicrobial resistance patterns. | the purpose of this study was to analyse the data on resistance in enterococcus faecalis and e. faecium isolated from urine samples of inpatients from january 2010 to december 2013. compared to e. faecalis, e. faecium isolates were significantly more resistant to ampicilin, nitrofurantoin, and ciprofloxacin in the antimicrobial agents evaluated. both species showed higher resistance to high-level gentamicin. the rate of vancomycin-resistant enterococci (vre) in e. faecium was higher as compared ... | 2015 | 24835052 |
| formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (apdt): studies on curcumin and curcuminoids lii. | bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (apdt) are needed. curcumin is under investigation as a potential photosensitizer in apdt. | 2015 | 24842546 |
| comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white-coloured mineral trioxide aggregate. | to comparatively examine the cell attachment, cytotoxicity, and antibacterial activity of radiopaque dicalcium silicate cement (rdsc) and proroot white-coloured mineral trioxide aggregate (wmta). | 2015 | 24802368 |
| clonal diversity in biofilm formation by enterococcus faecalis in response to environmental stress associated with endodontic irrigants and medicaments. | to determine whether clonal diversity within e. faecalis affects biofilm formation when exposed to antimicrobial compounds found in endodontic medicaments and irrigants. | 2015 | 24749689 |
| demography and burden of care associated with patients readmitted for urinary tract infection. | urinary tract infection (uti) is one of the most prevalent admission diagnoses in hospital-based clinical practice. despite its frequency, few data are available regarding its demographics and economic implications. | 2015 | 24863498 |
| characterisation of the antibacterial effect of polyethyleneimine nanoparticles in relation to particle distribution in resin composite. | to characterise the antibacterial effect of resin composite incorporating cross-linked quaternised polyethyleneimine (qpei) nanoparticles in relation to their distribution in the bulk material. | 2015 | 24881908 |
| assessment of dentinal tubule invasion capacity of enterococcus faecalis under stress conditions ex vivo. | to investigate the dentinal tubule invasion capacity of enterococcus faecalis under alkaline and energy starvation stress conditions. | 2015 | 24872016 |
| production of lactic acid using a new homofermentative enterococcus faecalis isolate. | lactic acid is an intermediate-volume specialty chemical for a wide range of food and industrial applications such as pharmaceuticals, cosmetics and chemical syntheses. although lactic acid production has been well documented, improved production parameters that lead to reduced production costs are always of interest in industrial developments. in this study, we describe the production of lactic acid at high concentration, yield and volumetric productivity utilizing a novel homofermentative, fac ... | 2015 | 24894833 |
| modified lactic acid bacteria detect and inhibit multiresistant enterococci. | we designed lactococcus lactis to detect enterococcus faecalis. upon detection, l. lactis produce and secrete antienterococcal peptides. the peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of l. lactis. the enterococcal sex pheromone ccf10 serves as the signal for detection. expression vectors derived from pcf10, a ccf10-responsive e. faecalis sex-pheromone conjugative plasmid, were engineered in l. lactis for the detection system. recombinant host strain ... | 2015 | 24896372 |
| comparison of apical sealing ability of two phases of gutta-percha: a bacterial leakage model. | the aim of this study was to compare apical sealing ability of alpha and beta phases of gutta-percha by means of bacterial leakage model. | 2015 | 27507995 |
| effect of different obturation materials on residual antimicrobial activity of 2% chlorhexidine in dentin at different time intervals: an ex vivo study. | the aim of this study was to evaluate the effect of gutta percha/ah26 and resilon/realseal se on residual antimicrobial activity of chlorhexidine (chx) in human root dentin and suggest the best filling material when chx is used as final irrigant. | 2015 | 27252755 |
| enterococcus faecalis biofilm. formation and development in vitro observed by scanning electron microscopy. | biofilm produced by enterococcus faecalis isolated from root canals was detected by growing it on microplates and using 10% crystal violet stain, elution with alcohol and three procedures: no fixation, heat fixation and 10% formaldehyde fixation. the biofilm was evaluated using a versamax microplate reader (usa). twenty sterile root portions were incubated in ts broth with e. faecalis (108) for 48 hours, 4, 7, 14 and 30 days, after which they were processed and observed by scanning electron micr ... | 2015 | 27095620 |
| characterization of enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production. | in this study, 72 enterococcus faecalis isolates originating from humans (n=39), dogs (n=26) and cats (n=7) were investigated for some virulence factors, some virulence genes, antibiotic resistance phenotypes, randomly amplified polymorphic dna-polymerase chain reaction (rapd-pcr) patterns and biofilm production. of the isolates, 31 (43.1%) were positive for gelatinase, 11 (15.3%) for aggregation substance and cytolysine, 38 (52.8%) for gele and 34 (47.2%) for asa1 genes. all isolates were found ... | 2015 | 27175186 |
| antimicrobial activity of herbal medicines (tulsi extract, neem extract) and chlorhexidine against enterococcus faecalis in endodontics: an in vitro study. | successful endodontic treatment depends on effective disinfection and complete sealing of root canal. various medicaments are advised for disinfecting root canal, such as herbal and non-herbal medicaments. this study was done to assess the antimicrobial activity of herbal medicines (neem extract, tulsi extract) and chlorhexidine against enterococcus faecalis in endodontics. | 2015 | 26942123 |
| [clinical observations of acute-onset endophthalmitis after clear corneal phacoemulsification]. | to evaluate the incidence of acute-onset endophthalmitis after clear corneal phacoemulsification and to report its clinical features, microbiology, and therapeutic efficacy. | 2015 | 26888274 |
| cholesterol suppresses antimicrobial effect of statins. | isoprenoid biosynthesis is a key metabolic pathway to produce a wide variety of biomolecules such as cholesterol and carotenoids, which target cell membranes. on the other hand, it has been reported that statins known as inhibitors of isoprenoid biosynthesis and cholesterol lowering agents, may have a direct antimicrobial effect on the some bacteria. the exact action of statins in microbial metabolism is not clearly understood. it is possible that statins inhibit synthesis or utilization of some ... | 2015 | 26877857 |
| did the iceman know better? screening of the medicinal properties of the birch polypore medicinal mushroom, piptoporus betulinus (higher basidiomycetes). | the birch polypore piptoporus betulinus was among two mushrooms that were found in the iceman's bag. recent studies indicated that p. betulinus was probably used as a religious and medicinal item. in order to examine the medicinal potential of p. betulinus, hot water (hw), partially purified (pp), and alkali extract (ha) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin i-converting enzyme (ace) inhibitory activity. all tested samples exhibited moderate cytotoxi ... | 2015 | 26854098 |
| enterococcus faecalis from food, clinical specimens, and oral sites: prevalence of virulence factors in association with biofilm formation. | enterococci have gained significance as the cause of nosocomial infections; they occur as food contaminants and have also been linked to dental diseases. e. faecalis has a great potential to spread virulence as well as antibiotic resistance genes via horizontal gene transfer. the integration of food-borne enterococci into the oral biofilm in-vivo has been observed. therefore, we investigated the virulence determinants and antibiotic resistance of 97 e. faecalis isolates from the oral cavity, foo ... | 2015 | 26793174 |
| global regulation of gene expression by the mafr protein of enterococcus faecalis. | enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. however, as an opportunistic pathogen, it is able to colonize other host niches and cause life-threatening infections. its adaptation to new environments involves global changes in gene expression. the ef3013 gene (here named mafr) of e. faecalis strain v583 encodes a protein (mafr, 482 residues) that has sequence similarity to global response regulators of the mga/atxa family. the enterococcal og1rf genome also e ... | 2015 | 26793169 |
| [the incidence and risk factors of male urinary tract infection following invasive urodynamic investigation]. | to assess the prevalence of male urinary tract infection (uti) after invasive urodynamic studies and to determinate the risk factors by evaluating the clinical characteristics and urodynamic parameters of patients. | 2015 | 26813139 |
| the influence of urinary tract infections on treatment of fracture of proximal femur in patients with systemiv osteoporosis. | in the structure of intrahospital infections the infections of urinary tract make up from 20 to 40%. this problem represents special medical-social significance for trauma departments as patients with trauma are being in forced situation for along time. 380 patients older than 60 years (average age 73.6 ± 9.2 years) with a fracture of proximal femur against background of the systemic osteoporosis having in-patient treatment in the trauma department of the republic hospital no2 were being examine ... | 2015 | 26887137 |
| enterococcus faecalis as multidrug resistance strains in clinical isolates in imam reza hospital in kermanshah, iran. | the current study aimed to investigate the prevalence of vancomycin-resistant enterococcus in e. faecalis and e. faecium and antimicrobial susceptibility patterns, then dominant genes responsible for vancomycin resistance were determined. for this propose, 180 clinical isolates of enterococcus were subjected for identification and antibiotic susceptibility assay. then, the gene responsible vancomycin resistant strains were determined. the results demonstrated the e. faecalis as a dominant entero ... | 2015 | 26738400 |
| [a case of pyoderma gangrenosum of the penis difficult to distinguish from fournier gangrene]. | here, we report a case of pyoderma gangrenosum of the penis which was difficult to distinguish from fournier gangrene. the patient was a 54-year-old male who was aware of redness and swelling of the glanspenis for 1 month prior to a consultation at our department. although he was diagnosed with herpes and treated at a nearby hospital, his symptoms did not improve. subsequently, the patient visited our department following the onset of pain and fever. during his initial consultation, he had a fev ... | 2015 | 26699892 |
| evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of enterococcus faecalis. | background and aims. triple antibiotic paste (tap) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. the aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of tap at 1-, 2-, 3-, and 4-week intervals on mature enterococcus faecalis biofilm. materials and methods. a total of 287 extracted one-rooted human central incisors were infected with e. faecalis atcc 29212 after removing the crown and preparation ... | 2015 | 26697145 |
| the capability of tyramine production and correlation between phenotypic and genetic characteristics of enterococcus faecium and enterococcus faecalis strains. | the aim of this study was to investigate the diversity of tyramine production capability of four enterococcus strains in buffered systems in relation to their genetic characteristics and environmental conditions. cells of the strains enterococcus faecalis ef37 and atcc 29212, and e. faecium fc12 and fc643 were re-suspended in phosphate/citrate buffers with different ph, nacl concentration and incubation temperature. at intervals, cell viability and tyramine production were assessed by plate coun ... | 2015 | 26696981 |
| olive mill waste extracts: polyphenols content, antioxidant, and antimicrobial activities. | natural polyphenols extracts have been usually associated with great bioactive properties. in this work, we investigated in vitro antioxidant and antimicrobial potential of the phenolic olive mill wastewater extracts (owwe) and the olive cake extracts (oce). using the folin ciocalteux method, owwe contained higher total phenol content compared to oce (8.90 ± 0.728 g/l versus 0.95 ± 0.017 mg/g). the phenolic compounds identification was carried out with a performance liquid chromatograph coupled ... | 2015 | 26693221 |
| enterococcus faecalis septicemia and vertebral osteomyelitis after transrectal ultrasound guided biopsy of the prostate. | transrectal ultrasound guided prostate biopsy (trus) has rarely been associated with disseminated infection, yet the occurrence appears to be increasing. resistance to fluoroquinolones, the most commonly used prophylaxis, is one of the likely causes, with escherichia coli being the most commonly reported cause of these infections. herein we present what is, to our knowledge, the first case of enterococcus faecalis septicemia and vertebral osteomyelitis after trus. previously reported cases of th ... | 2015 | 26682075 |
| genomic sequence of a clinical vancomycin-resistant reference strain, enterococcus faecalis atcc 51299. | in this paper, we present a draft genome sequence of a quality control reference strain, enterococcus faecalis atcc 51299 (multilocus sequencing type [mlst] st6), which is sensitive to teicoplanin but resistant to vancomycin. it is used in an agar screening test for streptomycin, gentamicin, and vancomycin resistance and the resistance marker vanb. | 2015 | 26679597 |
| an in vitro evaluation of antimicrobial activity of five herbal extracts and comparison of their activity with 2.5% sodium hypochlorite against enterococcus faecalis. | sodium hypochlorite is the most widely used irrigant in endodontic practice, but it has various disadvantages. literature has shown that herbal products such as propolis, azadirachta indica (ai), triphala, curcuma longa, and morinda citrifolia (mc) possess good antimicrobial properties and thus can be used as potential endodontic irrigants. | 2015 | 26672425 |
| anti-microbial efficacy of allium sativum extract against enterococcus faecalis biofilm and its penetration into the root dentin: an in vitro study. | sodium hypochlorite (naocl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effective led to this study. | 2015 | 26672416 |
| the effect of addition of an eps degrading enzyme with and without detergent to 2% chlorhexidine on disruption of enterococcus faecalis biofilm: a confocal laser scanning microscopic study. | enterococcus faecalis is one of the most commonly occurring organisms retrieved from root canal treated teeth that show refractory apical periodontitis. though it is well known that the ability of e. faecalis to form a matrix-encased biofilm contributes to its pathogenicity, the role of extracellular dextran and dna in biofilm formation and its effect on the susceptibility of the biofilm to chlorhexidine remains poorly understood. it was hypothesized that the addition of an extracellular polymer ... | 2015 | 26675655 |
| hydrothermally treated chitosan hydrogel loaded with copper and zinc particles as a potential micronutrient-based antimicrobial feed additive. | large-scale use of antibiotics in food animal farms as growth promoters is considered as one of the driving factors behind increasing incidence of microbial resistance. several alternatives are under investigation to reduce the amount of total antibiotics used in order to avoid any potential transmission of drug resistant microbes to humans through food chain. copper sulfate and zinc oxide salts are used as feed supplement as they exhibit antimicrobial properties in addition to being micronutrie ... | 2015 | 26664989 |
| degree of bacterial microleakage at the implant-abutment junction in cone morse tapered implants under loaded and unloaded conditions. | different results have been reported on the internal colonization of cone morse connections under in vitro dynamic loading. the aim of the present in vitro study was to evaluate the bacterial leakage in cone morse implant-abutment connections, both under loaded and unloaded conditions. | 2015 | 26660625 |
| tricuspid valve endocarditis complicated by mobitz type ii heart block - a case report and literature review. | we present a case of a middle-aged male who manifested with low-grade fever and lower back pain. mri and bone scan of the spine were suggestive of vertebral osteomyelitis. blood cultures were persistently positive for enterococcus faecalis and echocardiogram revealed tricuspid valve endocarditis. there was no history of iv drug use and urine toxicology was negative. ekg showed mobitz type ii av block and a transesophageal echocardiogram revealed no valve ring or septal abscesses. the heart block ... | 2015 | 26653699 |
| antibacterial and toxic effect of hydrogen peroxide combined with different concentrations of chlorhexidine in comparison with sodium hypochlorite. | hydrogen peroxide (h2o2) has been suggested to be used in sequence or in combination with chlorhexidine (chx) to enhance the antibacterial activity against enterococcus faecalis, but there is no research in the literature on the safety and effectiveness of this irrigation protocol. | 2015 | 26636124 |
| in vitro comparison of antimicrobial effectiveness of qmix and other final irrigants in human root canals. | final root canal irrigation stands as an effective strategy for eliminating the dentin infection. this study aimed to investigate and compare the antibacterial efficacy of qmix and other four final irrigation regimens in reducing enterococcus faecalis within human root canals. single-canal human teeth contaminated with e. faecalis for 4 weeks were prepared chemomechanically with sodium hypochlorite (naocl). then, the teeth were randomly assigned into six groups according to the final irrigation ... | 2015 | 26631907 |
| clinical and microbiological features of bacteremia caused by enterococcus faecalis. | enterococcus faecalis is a frequent etiologic agent of invasive infections in hospitalized patients. the aim of this study was to analyze clinical and microbiological features of bacteremia caused by e. faecalis. | 2015 | 26623628 |
| synergistic antibacterial effect of the combination of ε-polylysine and nisin against enterococcus faecalis. | this study evaluated the antibacterial effect of the combination of ε-polylysine (ε-pl) and nisin against enterococcus faecalis strains. the combination of ε-pl and nisin showed synergistic antibacterial activity against three enterococcus strains. scanning electron microscopy and a membrane permeability assay revealed that the combined treatment with ε-pl and nisin synergistically damaged the cell morphology of e. faecalis strain r612z1 cells. both ε-pl and nisin can dissipate the transmembrane ... | 2015 | 26613915 |
| infective endocarditis involving the pulmonary valve. | pulmonary valve (pv) infective endocarditis (ie) is a rare entity, accounting for 1.5% to 2% of cases of ie. published data are limited to a few case series and reports. we sought to review the mayo clinic experience and describe clinical, echocardiographic, and microbiologic features. we included all patients aged ≥18 years seen from 2000 to 2014 who had a diagnosis of native pv ie and unequivocal echocardiographic involvement of the pv. nine patients with pv ie were identified. isolated pv ie ... | 2015 | 26611123 |
| biological changes of enterococcus faecalis in the viable but nonculturable state. | enterococcus faecalis may enter a viable but nonculturable (vbnc) state under adverse conditions. e. faecalis, the major bacterial species present in failed root canal treatments, is thought to survive after endodontic treatment by entering a vbnc state. in this study, we characterized the vbnc state of e. faecalis. we designed 3 different protocols to successfully induce the vbnc state. approximately one-third of bacteria entered a vbnc state after 15-30 days, and all remained viable for at lea ... | 2015 | 26600540 |
| antibiotic resistance and biofilm formation of enterococcus faecalis in patient and environmental samples. | enterococci are opportunistic pathogens and are a major factor in nosocomial infections. they may contain ebp operon, which upon expression makes them highly prone to biofilm formation on biotic and abiotic surfaces. | 2015 | 26587208 |
| assessment of the potential of cfc (calcium hydroxide flagyl ciprofloxacin) for the rapid disinfection of resilon and gutta-percha. | obturating materials exposed to the dental operating environment has been shown to be contaminated, making rapid chair side disinfection mandatory to ensure the sterility of the root canals. | 2015 | 26557614 |
| actions of probiotics on trinitrobenzenesulfonic acid-induced colitis in rats. | we investigated the actions of probiotics, streptococcus faecalis 129 bio 3b (sf3b), in a trinitrobenzenesulfonic acid- (tnbs-) induced colitis model in rats. after tnbs was administered into the colons of rats for induction of colitis, the rats were divided into two groups: one group was given a control diet and the other group was given a diet containing sf3b for 14 days. there were no apparent differences in body weight, diarrhea period, macroscopic colitis score, and colonic weight/length ra ... | 2015 | 26550572 |
| in vitro activity of oritavancin and comparator agents against staphylococci, streptococci and enterococci from clinical infections in europe and north america, 2011-2014. | oritavancin is a lipoglycopeptide that has been approved for the treatment of acute bacterial skin and skin-structure infections (absssis) caused by susceptible organisms. oritavancin causes cell death by inhibiting cell wall synthesis as well as depolarising and permeabilising the cellular membrane of gram-positive pathogens. the activities of oritavancin in comparison with vancomycin, daptomycin and linezolid were determined against a collection of over 11000 recent clinical gram-positive isol ... | 2015 | 26541881 |
| comparative evaluation of effect of different irrigation solutions against enterococcus faecalis: a polymerase chain reaction-based study. | enterococcus faecalis is the most isolated or detected species from oral infections including marginal periodontitis, infected root canals, periradicular abscesses and also detected in cases of failed endodontic therapy. to prevent endodontic treatment failure irrigation is mandatory for the effective removal of smear layer, pulp tissue, and microorganisms. cultivation and other traditional identification methods have been demonstrated to have several limitations when it comes to microbiological ... | 2015 | 26538921 |
| microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation. | this study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (cmp). | 2015 | 26521147 |
| influence of a subinhibitory concentration of vancomycin on the in vitro expression of virulence-related genes in the vancomycin-resistant enterococcus faecalis. | exposure to subinhibitory concentrations (sics) of antimicrobials may alter the bacterial transcriptome. | 2015 | 26516976 |
| rapidly progressive gas-containing lumbar spinal epidural abscess. | gas-containing (emphysematous) infections of the abdomen, pelvis, and extremities are well-known disease entities, which can potentially be life-threatening. they require aggressive medical and often surgical treatment. in the neurosurgical field, some cases of gas-containing brain abscess and subdural empyema have been reported. sometimes they progress rapidly and even can cause fatal outcome. however, gas-containing spinal epidural abscess has been rarely reported and clinical course is unknow ... | 2015 | 26512268 |
| design and activity of a cyclic mini-β-defensin analog: a novel antimicrobial tool. | we have designed a cyclic 17-amino acid β-defensin analog featuring a single disulfide bond. this analog, designated "amc" (ie, antimicrobial cyclic peptide), combines the internal hydrophobic domain of hbd1 and the c-terminal charged region of hbd3. the novel peptide was synthesized and characterized by nuclear magnetic resonance spectroscopy. the antimicrobial activities against gram-positive and gram-negative bacteria as well as against herpes simplex virus type 1 were analyzed. the cytotoxic ... | 2015 | 26508857 |
| structural analysis of streptococcus pyogenes nadh oxidase: conformational dynamics involved in formation of the c(4a)-peroxyflavin intermediate. | in probing the oxygen reactivity of an enterococcus faecalis nadh oxidase (nox; o2 → 2h2o) c42s mutant lacking the cys42-sulfenic acid (cys42-soh) redox center, we provided direct evidence of a c(4a)-peroxyflavin intermediate in the oxidative half-reaction and also described a conformational or chemical change that is rate-limiting for full reoxidation of the homodimer. in this work, the nox from streptococcus pyogenes (spynox) has been expressed and crystallized, and the overoxidized wild-type ... | 2015 | 26506002 |
| functional analysis of genes involved in the biosynthesis of enterocin nkr-5-3b, a novel circular bacteriocin. | a putative biosynthetic gene cluster of the enterocin nkr-5-3b (ent53b), a novel circular bacteriocin, was analyzed by sequencing the flanking regions around enkb, the ent53b structural gene, using a fosmid library. a region approximately 9 kb in length was obtained, and the enkb1, enkb2, enkb3, and enkb4 genes, encoding putative biosynthetic proteins involved in the production, maturation, and secretion of ent53b, were identified. we also determined the identity of proteins mediating self-immun ... | 2015 | 26503847 |
| activity of fosfomycin and comparison of several susceptibility testing methods against contemporary urine isolates. | fosfomycin is recommended as first-line treatment for acute uncomplicated cystitis in women. it has demonstrated in vitro activity against a variety of pathogens; however, a paucity of data are available from the usa. we determined the susceptibility of a collection of urine isolates to fosfomycin and compared multiple methods of susceptibility testing. consecutive non-duplicate enterobacteriaceae, enterococci and pseudomonas aeruginosa isolates were collected from the clinical microbiology labo ... | 2015 | 26498988 |
| selective digestive decontamination and enterococcus faecalis overgrowth and infection: a robust relation is yet unproven. | 2015 | 26495899 | |
| virus satellites drive viral evolution and ecology. | virus satellites are widespread subcellular entities, present both in eukaryotic and in prokaryotic cells. their modus vivendi involves parasitism of the life cycle of their inducing helper viruses, which assures their transmission to a new host. however, the evolutionary and ecological implications of satellites on helper viruses remain unclear. here, using staphylococcal pathogenicity islands (sapis) as a model of virus satellites, we experimentally show that helper viruses rapidly evolve resi ... | 2015 | 26495848 |
| antibacterial efficacy of polymer containing nanoparticles in comparison with sodium hypochlorite in infected root canals. | the aim of the present study was to compare the antibacterial properties of plga polymer containing zno, ag and zno/ag nanoparticles with those of 2.5% naocl in root canals contaminated with enterococcus faecalis. | 2015 | 26486202 |
| antimicrobial activity of silver doped fabrics for the production of hospital uniforms. | among several alternatives to control hospital-acquired infections (hais), a strategy could be the use of hospital uniforms imbued with antimicrobial substances. for this purpose we evaluated the antibacterial activity of two different silver doped fabrics employed for the production of hospital uniforms. the study was conducted in two-step. in the first the antimicrobial activity was evaluated in vitro against escherichia coli atcc 25922, pseudomonas aeruginosa atcc 27853, staphylococcus aureus ... | 2015 | 26485013 |
| bacteriocin production augments niche competition by enterococci in the mammalian gastrointestinal tract. | enterococcus faecalis is both a common commensal of the human gastrointestinal tract and a leading cause of hospital-acquired infections. systemic infections with multidrug-resistant enterococci occur subsequent to gastrointestinal colonization. preventing colonization by multidrug-resistant e. faecalis could therefore be a valuable approach towards limiting infection. however, little is known about the mechanisms e. faecalis uses to colonize and compete for stable gastrointestinal niches. phero ... | 2015 | 26479034 |
| modeling and application of a rapid fluorescence-based assay for biotoxicity in anaerobic digestion. | the sensitivity of anaerobic digestion metabolism to a wide range of solutes makes it important to be able to monitor toxicants in the feed to anaerobic digesters to optimize their operation. in this study, a rapid fluorescence measurement technique based on resazurin reduction using a microplate reader was developed and applied for the detection of toxicants and/or inhibitors to digesters. a kinetic model was developed to describe the process of resazurin reduced to resorufin, and eventually to ... | 2015 | 26457928 |
| tumor necrosis factor receptor-associated factor 6 plays a role in the inflammatory responses of human periodontal ligament fibroblasts to enterococcus faecalis. | enterococcus faecalis is a frequently isolated microorganism in persistent periapical lesion or secondary infection. however, no evidence has demonstrated that e. faecalis induced inflammation directly in the apical area. this study aimed to explore the mechanism of the inflammatory responses of human periodontal ligament fibroblasts (pdls) to e. faecalis. | 2015 | 26454720 |
| characterization of mobile staphylococcus equorum plasmids isolated from fermented seafood that confer lincomycin resistance. | the complete nucleotide sequences of lincomycin-resistance gene (lnua)-containing plasmids in staphylococcus equorum strains isolated from the high-salt-fermented seafood jeotgal were determined. these plasmids, designated pselnu1-3, are 2638-bp long, have two polymorphic sites, and encode typical elements found in plasmids that replicate via a rolling-circle mechanism including the replication protein gene (rep), a double-stranded origin of replication, a single-stranded origin of replication, ... | 2015 | 26448648 |
| labdane and clerodane diterpenoids from colophospermum mopane. | five labdane (1-5), an isolabdane (6), and five clerodane diterpenoids (7-11), were isolated from seeds, husks, and leaves of colophospermum mopane. compounds 1-3 and 6-9 are new, and their structures were elucidated by means of physical data analysis (1d and 2d nmr, hresims). the absolute configurations of 1, 7, and 10 were determined by single-crystal x-ray diffraction with cu kα radiation. for compounds 2 and 6, the absolute configurations were established by the modified mosher's method and ... | 2015 | 26444248 |
| faster and economical screening for vancomycin-resistant enterococci by sequential use of chromogenic agar and real-time polymerase chain reaction. | screening for vancomycin-resistant enterococci (vre) by culture takes days to generate results, while polymerase chain reaction (pcr) testing directly from clinical specimens lacks specificity. the aims of this study were to develop a real-time pcr to detect and identify enterococcus faecium, enterococcus faecalis, and vana and vanb genes, and to evaluate the impact of this pcr on test-reporting times when performing it directly from suspect vre isolates present on screening chromogenic media. | 2015 | 26442675 |
| clinico-microbiological investigation of catheter associated urinary tract infection by enterococcus faecalis: vana genotype. | prolonged hospitalization and exposure to third generation cephalosporins are reported to facilitate the acquisition and colonization of vancomycin resistant enterococci (vre). though vre is not uncommon in india, urinary tract infection with a vana genotype is a cause of serious concern as vre co-exhibit resistance to aminoglycosides. in india, majority of the vre isolates recovered from hospitalized patients include enterococcus faecium. we report a case of catheter associated urinary tract in ... | 2015 | 26435949 |
| synthesis, antimycobacterial and antibacterial activity of fluoroquinolone derivatives containing an 3-alkoxyimino-4-(cyclopropylanimo)methylpyrrolidine moiety. | a series of novel fluoroquinolone derivatives containing an 3-alkoxyimino-4-(cyclopropylanimo)methylpyrrolidine moiety were designed, synthesized and evaluated for their biological activity. our results revealed that 19b2 shows good activity against mtb h37rv atcc 27294 (mic: <0.25 μg/ml) and mdr-mtb 6133 clinical isolate (mic: 0.11 μg/ml). most of them have potent potency against gram-positive strains, although they are generally poor active against gram-negative strains. especially, compounds ... | 2015 | 26435513 |
| a comparison of apical bacterial extrusion in manual, protaper rotary, and one shape rotary instrumentation techniques. | apical extrusion of irrigants and debris is an inherent limitation associated with cleaning and shaping of root canals and has been studied extensively because of its clinical relevance as a cause of flare-ups. many factors affect the amount of extruded intracanal materials. the purpose of this study was to assess the bacterial extrusion by using manual, multiple-file continuous rotary system (protaper) and single-file continuous rotary system (one shape). | 2015 | 26472680 |
| clinical characteristics and risk factors of enterococcal infections in nagasaki, japan: a retrospective study. | enterococcus spp. are particularly important etiological agents of nosocomial infections. however, the clinical characteristics of and risk factors for enterococcal infections in clinical settings are poorly understood. | 2015 | 26471715 |
| preliminary evaluation of the research-use-only (ruo) icubate ic-gpc assay for identification of select gram-positive bacteria and their resistance determinants in blood culture broths. | the ic-gpc assay (icubate, huntsville, al) provides a molecular option for the rapid, on-demand analysis of positive blood cultures. a preliminary evaluation of the ic-gpc assay using 203 clinical or seeded specimens demonstrated a sensitivity of 93.8% to 100% and a specificity of 98.0% to 100% for the identification of five gram-positive bacterial species (staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, enterococcus faecalis, and enterococcus faecium) and three asso ... | 2015 | 26468498 |
| prevalence, seasonality, and growth of enterococci in raw and pasteurized milk in victoria, australia. | this study investigated the prevalence, seasonality, and species variety of enterococci present in raw milk factory silos and pasteurized milk in 3 dairying regions in victoria, australia, over a 1-yr period. additionally, the growth ability of thermoduric enterococci isolated in this study (enterococcus faecalis, e. faecium, e. hirae, and e. durans) was determined in milk at temperatures likely to occur during storage, transport, and distribution, and before domestic consumption (4 and 7°c). en ... | 2015 | 26476940 |
| antimicrobial effect against different bacterial strains and bacterial adaptation to essential oils used as feed additives. | the aim of this study was to evaluate the antimicrobial activity and determine the minimum bactericidal concentration (mbc) of the essential oils derived from origanum vulgare (oregano), melaleuca alternifolia (tea tree), cinnamomum cassia (cassia), and thymus vulgaris (white thyme) against salmonella typhimurium, salmonella enteritidis, escherichia coli, staphylococcus aureus and enterococcus faecalis. the study also investigated the ability of these different bacterial strains to develop adapt ... | 2015 | 26424908 |
| antimicrobial activity and ph of calcium hydroxide and zinc oxide nanoparticles intracanal medication and association with chlorhexidine. | to evaluate ph and antibacterial activity of pastes with calcium hydroxide [ca(oh)2] and zinc oxide (zno) microparticles (micro) or nanoparticles (nano) and association with 0.4% chlorhexidine against enterococcus faecalis. | 2015 | 26423497 |
| orthopedic implant-infections. incompetence of staphylococcus epidermidis, staphylococcus lugdunensis and enterococcus faecalis to invade osteoblasts. | septic failure is still the major complication of prosthetic implants. entering host cells, bacteria hide from host immune defenses, shelter from extracellular antibiotics, and cause chronic infection. staphylococcus aureus, the leading etiologic agent of orthopedic implant-infections, is able to enter bone cells and induce osteoblast apoptosis, osteoclast recruitment, and highly destructive osteomyelitis. staphylococcus epidermidis, staphylococcus lugdunensis and enterococcus faecalis are oppor ... | 2015 | 26378773 |
| in vitro studies of antibacterial and antifungal wound dressings comprising h2tio3 and sio2 nanoparticles. | the incidence rate of the infected and complex wound is established at approximately 40,000/1 million of the world's adult population. the aim of this study was to assess the efficiency of three novel types of wound dressings comprising sodium chloride, metatitanic acid and silicon dioxide nanoparticles. the study design was to prove their antimicrobial properties against the microorganisms most commonly causing wound infections. the study evaluated the antimicrobial effect of tested dressings o ... | 2015 | 26373173 |
| antibacterial, antifungal and antimycobacterial activities of some pyrazoline, hydrazone and chalcone derivatives. | twenty-seven previously reported chalcones and their pyrazoline and hydrazone derivatives as well as two further chalcones have been screened for their antimicrobial, antifungal and antimycobacterial activities against standard microbial strains and drug resistant isolates. the minimum inhibitory concentration (mic) value of each compound was determined by a two-fold serial microdilution technique. the compounds were found to possess a broad spectrum of antimicrobial activities with mic values o ... | 2015 | 26372110 |
| effects of pre-encapsulated and pro-encapsulated enterococcus faecalis on growth performance, blood characteristics, and cecal microflora in broiler chickens. | the effects of microencapsulation of enterococcus faecalis on the growth performance, antioxidant activity, immune function, and cecal microbiota in broilers were investigated. broilers (1-day-old) were assigned randomly as follows: 5 treatments, 5 replicate pens per treatment, and 20 broilers per pen. treatments included (1) a basal diet (con), (2) con + aureomycin (1 g/kg of diet) (ant), (3) con + free non-encapsulated probiotics (1 × 10(9) cfu/kg of diet) (free), (4) con + pro-encapsulated pr ... | 2015 | 26371326 |
| deletion of liar reverses daptomycin resistance in enterococcus faecium independent of the genetic background. | we have shown previously that changes in liafsr, a three-component regulatory system predicted to orchestrate the cell membrane stress response, are important mediators of daptomycin (dap) resistance in enterococci. indeed, deletion of the gene encoding the response regulator liar in a clinical strain of enterococcus faecalis reversed dap resistance (dap-r) and produced a strain hypersusceptible to antimicrobial peptides. since liafsr is conserved in enterococcus faecium, we investigated the rol ... | 2015 | 26369959 |
| chemical compositions and aroma evaluation of volatile oil from the industrial cultivation medium of enterococcus faecalis. | enterococcus faecalis is one of the major lactic acid bacterium (lab) species colonizing the intestines of animals and humans. the characteristic odor of the volatile oils obtained from both the liquid medium after incubation (mai) and liquid medium before incubation (mbi) in the cultivation process of e. faecalis was investigated to determine the utility of the liquid medium. in total, fifty-six and thirty-two compounds were detected in the volatile oils from the mai (mai oil) and mbi (mbi oil) ... | 2015 | 26369592 |
| inhibitory effect of gels loaded with a low concentration of antibiotics against biofilm formation by enterococcus faecalis and porphyromonas gingivalis. | we explored longitudinally the inhibitory effect of gels loaded with 1 mg/ml modified triple antibiotic paste (mtap) or double antibiotic paste (dap) against biofilm formation by enterococcus faecalis and porphyromonas gingivalis. methylcellulose-based antibiotic gels of mtap (ciprofloxacin, metronidazole and clindamycin) and dap (ciprofloxacin and metronidazole) were prepared at a concentration of 1 mg/ml. individually cultured e. faecalis and p. gingivalis bacterial suspensions were treated wi ... | 2015 | 26369485 |
| erratum to: investigating the mobilome in clinically important lineages of enterococcus faecium and enterococcus faecalis. | 2015 | 26369411 | |
| the effects of indoor and outdoor dust exposure on the growth, sensitivity to oxidative-stress, and biofilm production of three opportunistic bacterial pathogens. | within the last decade, many studies have highlighted the radical changes in the components of indoor and outdoor dust. for example, agents like automobile emitted platinum group elements and different kinds of organic phthalates and esters have been reported to be accumulating in the biosphere. humans consistently face dermal, respiratory, and dietary exposures to these particles while indoors and outdoors. in fact, dust particulate matter has been associated with close to 500,000 deaths per ye ... | 2015 | 26363607 |
| purple urine bag syndrome in two elderly men with urinary tract infection. | purple urine bag syndrome is a rare condition in which purple discoloration of urine inside its collection bag occurs. we describe two illustrative cases. the first patient is an 81-year-old man who was hospitalized for a newly diagnosed lymphoma with acute obstructive renal failure for which a nephrostomy procedure was performed. during the hospitalization, a sudden purple discoloration of the suprapubic catheter urine was noted, while the nephrostomy urine had a normal color. urine culture fro ... | 2015 | 26351597 |
| the fibronectin-binding protein efba contributes to pathogenesis and protects against infective endocarditis caused by enterococcus faecalis. | efba is a pava-like fibronectin adhesin of enterococcus faecalis previously shown to be important in experimental urinary tract infection. here, we expressed and purified the e. faecalis og1rf efba and confirmed that this protein binds with high affinity to immobilized fibronectin, collagen i, and collagen v. we constructed an efba deletion mutant and demonstrated that its virulence was significantly attenuated (p < 0.0006) versus the wild type in a mixed inoculum rat endocarditis model. further ... | 2015 | 26351286 |
| enterococcus faecalis-related prostatitis successfully treated with moxifloxacin. | 2015 | 26349832 | |
| structure-activity relationship of the aminomethylcyclines and the discovery of omadacycline. | a series of novel tetracycline derivatives were synthesized with the goal of creating new antibiotics that would be unaffected by the known tetracycline resistance mechanisms. new c-9-position derivatives of minocycline (the aminomethylcyclines [amcs]) were tested for in vitro activity against gram-positive strains containing known tetracycline resistance mechanisms of ribosomal protection (tet m in staphylococcus aureus, enterococcus faecalis, and streptococcus pneumoniae) and efflux (tet k in ... | 2015 | 26349824 |
| antimicrobial resistance and virulence factor gene profiles of enterococcus spp. isolates from wild arctocephalus australis (south american fur seal) and arctocephalus tropicalis (subantarctic fur seal). | enterococci are natural inhabitants of the gastrointestinal tracts in humans and animals. epidemiological data suggest that enterococci are important reservoirs of antimicrobial resistant genes that may be transmitted from other bacterial species the aim of this study was to investigate the species composition, antimicrobial resistance and virulence genes in enterococci recovered from fecal samples of wild arctocephalus australis and a. tropicalis found dead along the south coast of brazil. from ... | 2015 | 26347323 |
| chemical composition and in vitro antibacterial activity of the essential oil of verbesina negrensis from the venezuelan andes. | the composition and antimicrobial activity of the essential oil from verbesina negrensis steyerm. leaves is reported. analysis was performed by gc/ms. major constituents were α-pinene (43.1%), α-humulene (13.8%), δ-cadinene (8.1%), limonene (4.6%) and bicyclogermacrene (4.2%). the essential oil showed in vitro activity against the gram-positive bacteria staphylococcus aureus atcc 25923 and enterococcus faecalis atcc 29212 (mic 500 and 350 μl/ml respectively). | 2015 | 26411037 |
| lipoteichoic acid from an enterococcus faecalis clinical strain promotes tnf-α expression through the nf-κb and p38 mapk signaling pathways in differentiated thp-1 macrophages. | to study the immune-inflammatory response and signaling mechanism of macrophages to purified enterococcus faecalis (e. faecalis) lipoteichoic acid (lta), intact lta was obtained from an e. faecalis clinical strain p25rc using the butanol method and hydrophobic interaction chromatography purification. the fractions containing lta were determined using phosphate detection. contaminations with lipopolysaccharide and proteins were excluded using the limulus amoebocyte lysate assay and sodium dodecyl ... | 2015 | 26405548 |
| comparative analysis of the orphan crispr2 locus in 242 enterococcus faecalis strains. | clustered, regularly interspaced short palindromic repeats and their associated cas proteins (crispr-cas) provide prokaryotes with a mechanism for defense against mobile genetic elements (mges). a crispr locus is a molecular memory of mge encounters. it contains an array of short sequences, called spacers, that generally have sequence identity to mges. three different crispr loci have been identified among strains of the opportunistic pathogen enterococcus faecalis. crispr1 and crispr3 are assoc ... | 2015 | 26398194 |
| surveillance of gram-positive cocci infections and drug resistance. | in this study, the prevalence of gram-positive cocci isolates and the characteristics of multiple drug resistances in patients were investigated. antibiotic resistances were determined in the clinical microbiology laboratory with the methodology of the clsi (2012). the software whonet5.4 and spss13.0 were used for statistical analysis. there were a total of 6211 gram-positive cocci isolates, comprised of 2255 (36.3%) coagulase (-) staphylococci, 1277 (20.6%) staphylococci aureus, 1109 (17.9%) en ... | 2015 | 26386666 |
| [construction of luxs gene knockout mutant of enterococcus faecalis]. | to construct quorum sensing luxs knockout mutants of enterococcus faecalis through homologous recombination. | 2015 | 26383557 |
| comparative evaluation of antimicrobial activity of hydroalcoholic extract of aloe vera, garlic, and 5% sodium hypochlorite as root canal irrigants against enterococcus faecalis: an in vitro study. | enterococcus faecalis are the most resistant and predominant microorganisms recovered from root canals of teeth where previous treatment has failed. over the past decade, interest in drugs derived from medicinal plants has markedly increased. in dentistry, phytomedicines has been used as an anti-inflammatory, antibiotic, analgesic, sedative, and also as an endodontic irrigant. in endodontics, because of the cytotoxic reactions of most of the commercial intracanal medicaments and their inability ... | 2015 | 26381627 |
| characterization of sviceucin from streptomyces provides insight into enzyme exchangeability and disulfide bond formation in lasso peptides. | lasso peptides are bacterial ribosomally synthesized and post-translationally modified peptides. they have sparked increasing interest in peptide-based drug development because of their compact, interlocked structure, which offers superior stability and protein-binding capacity. disulfide bond-containing lasso peptides are rare and exhibit highly sought-after activities. in an effort to expand the repertoire of such molecules, we heterologously expressed, in streptomyces coelicolor, the gene clu ... | 2015 | 26343290 |
| rapid, culture-independent, optical diagnostics of centrifugally captured bacteria from urine samples. | this work presents a polymeric centrifugal microfluidic platform for the rapid and sensitive identification of bacteria directly from urine, thus eliminating time-consuming cultivation steps. this "lab-on-a-disc" platform utilizes the rotationally induced centrifugal field to efficiently capture bacteria directly from suspension within a glass-polymer hybrid chip. once trapped in an array of small v-shaped structures, the bacteria are readily available for spectroscopic characterization, such as ... | 2015 | 26339318 |
| transfer, composition and technological characterization of the lactic acid bacterial populations of the wooden vats used to produce traditional stretched cheeses. | the biofilms of 12 wooden vats used for the production of the traditional stretched cheeses caciocavallo palermitano and pdo vastedda della valle del belìce were investigated. salmonella spp. and listeria monocytogenes were never detected. total coliforms were at low numbers with escherichia coli found only in three vats. coagulase-positive staphylococci (cps) were below the enumeration limit, whereas lactic acid bacteria (lab) dominated the surfaces of all vats. in general, the dominance was sh ... | 2015 | 26338114 |
| the influence of different peritoneal dialysis fluids on the in vitro activity of ampicillin, daptomycin, and linezolid against enterococcus faecalis. | intraperitoneal administration of antibiotics is recommended for the treatment of peritoneal dialysis-related peritonitis. however, little data are available on a possible interference between peritoneal dialysis fluids and the activity of antimicrobial agents. thus, the present in vitro study set out to investigate the influence of different peritoneal dialysis fluids on the antimicrobial activity of ampicillin, linezolid, and daptomycin against enterococcus faecalis. time-kill curves in four d ... | 2015 | 26337433 |