Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antibody detection, isolation, genotyping, and virulence of toxoplasma gondii in captive felids from china. | the felids are the only definitive hosts of toxoplasma gondii, which could excrete oocysts into the environment and provide an infection source for toxoplasmosis in various warm-blooded animal species, particularly the captive felids that live close to human communities. the infection rate of the captive felids is a perfect standard in detecting the presence of toxoplasma gondii oocysts in the environment. in this study, sera or tissue samples from zoo (1 young tiger, 2 adult tigers, 6 young lio ... | 2017 | 28791002 |
| the use of elisa, npcr and qpcr for diagnosis of ocular toxoplasmosis in experimentally infected pigs. | in the present study we experimentally infected pigs with t. gondii tachyzoites, bradyzoites and oocysts in order to evaluate igg-elisa, nested-pcr, and qpcr for diagnosis of ocular infection. eighteen pigs were divided into four groups: g1 (infected with 10(3) tissue cysts of the m4 strain (type ii) at day 28, n=5), g2 (infected with 10(3) oocysts of the m4 strain at day 28, n=5), g3 (infected with tachyzoites of s48 strain (type 1) at day 0, n=5), and g4 (uninfected unchallenged, control group ... | 2017 | 28787692 |
| effects of recombinant toxoplasma gondii citrate synthase i on the cellular functions of murine macrophages in vitro. | toxoplasmosis, which is one of the most widespread zoonoses worldwide, has a high incidence and infection can result in severe disease in humans and livestock. citrate synthase (cs) is a component of nearly all living cells that plays a vital role in the citric acid cycle, which is the central metabolic pathway of aerobic organisms. in the present study, the citrate synthase i gene of toxoplasma gondii (t. gondii) (tgcsi) was cloned and characterized. the tgcsi gene had an open reading frame of ... | 2017 | 28785250 |
| elevated seroprevalence of toxoplasma gondii in aids/hiv patients: a meta-analysis. | clinical toxoplasmosis in aids/hiv patients is a great public health concern around the world. untreated toxoplasma gondii (t. gondii)-infections are often fatal in aids/hiv patients. this study aims to assess the seroprevalence and odds ratio (or) of t. gondii in aids/hiv patients, as well as the potential influential factors. studies published from december 1, 1983 to december 1, 2016 in english, which comparing the seroprevalence of t. gondii between aids/hiv patients and control group were s ... | 2017 | 28784422 |
| memory-phenotype cd4(+) t cells spontaneously generated under steady-state conditions exert innate th1-like effector function. | conventional cd4(+) t cells are composed of naïve, pathogen-specific memory, and pathogen-independent memory-phenotype (mp) cells under steady state. naïve and pathogen-specific memory cells play key roles in adaptive immunity, whereas the homeostatic mechanisms regulating the generation of mp cells and their biological functions are unclear. we show that mp cells are autonomously generated from peripheral naïve cells in the absence of infectious stimulation in a t cell receptor (tcr)- and cd28- ... | 2017 | 28783663 |
| seroprevalence of toxoplasma gondii infection in goats from the south-west region of poland and the detection of t. gondii dna in goat milk. | toxoplasma gondii (nicolle et manceaux, 1908) is an obligatory intracellular protozoan parasite prevalent in animals and humans worldwide having medical and veterinary importance on account of causing abortion or congenital disease in intermediate hosts, including man. since t. gondii has already been identified in the milk of goats, capra aegagrus hircus (linnaeus), the possibility of acquiring infection by ingesting unpasteurised goat milk should be taken into consideration. thus, the aim of t ... | 2017 | 28783032 |
| overcoming t. gondii infection and intracellular protein nanocapsules as biomaterials for ultrasonically controlled drug release. | one of the pivotal matters of concern in intracellular drug delivery is the preparation of biomaterials containing drugs that are compatible with the host target. nanocapsules for oral delivery are found to be suitable candidates for targeting toxoplasma gondii (t. gondii), a maneuvering and smart protozoic parasite found across europe and america that causes a subtle but deadly infection. to overcome this disease, there is much potential of integrating protein-based cells into bioinspired nanoc ... | 2017 | 28776612 |
| divergent co-transcriptomes of different host cells infected with toxoplasma gondii reveal cell type-specific host-parasite interactions. | the apicomplexan parasite toxoplasma gondii infects various cell types in avian and mammalian hosts including humans. infection of immunocompetent hosts is mostly asymptomatic or benign, but leads to development of largely dormant bradyzoites that persist predominantly within neurons and muscle cells. here we have analyzed the impact of the host cell type on the co-transcriptomes of host and parasite using high-throughput rna sequencing. murine cortical neurons and astrocytes, skeletal muscle ce ... | 2017 | 28775382 |
| activity of rosuvastatin in tachyzoites of toxoplasma gondii (rh strain) in hela cells. | due to the toxicity of conventional medication in toxoplasmosis, some drugs are being studied for treating this infection, such as statins, especially rosuvastatin compound, which is efficient in inhibiting the initial isoprenoid biosynthesis processes in humans and the parasite. the goal of this study was to assess the activity of rosuvastatin in hela cells infected with the rh strain of t. gondii. in the experiment, hela cells (1 × 10(5)) were infected with tachyzoites of t. gondii (5 × 10(5)) ... | 2017 | 28774497 |
| first description of enhanced expression of glia maturation factor-beta in experimantal toxoplasmic encephalitis. | objective we previously showed that toxoplasma gondii infection induces severe neuropathology in the form of oxidative stress, high nitric oxide production, glial activation, and apoptosis. this study examined the association between glia maturation factor-beta (gmf-β) expression, activated astrocytes/microglia, and neuropathology in toxoplasmic encephalitis (te). methods mouse brain gmf expression was examined by immunohistochemistry on days 10 and 30 post- t. gondii infection. results neuropat ... | 2017 | 28774213 |
| urban environments alter parasite fauna, weight and reproductive activity in the quenda (isoodon obesulus). | some wildlife species are capable of surviving in urbanised environments. however, the implications of urbanisation on wildlife health, and public health regarding zoonoses, are often unknown. quenda (syn. southern brown bandicoots, isoodon obesulus) survive in many areas of perth, australia, despite urbanisation. this study investigated differences in gastrointestinal and macroscopic ecto-parasitic infections, morphometrics and reproductive status between bushland and urban dwelling quenda. 287 ... | 2017 | 28764110 |
| pathogen-mediated nmda receptor autoimmunity and cellular barrier dysfunction in schizophrenia. | autoantibodies that bind the n-methyl-d-aspartate receptor (nmdar) may underlie glutamate receptor hypofunction and related cognitive impairment found in schizophrenia. exposure to neurotropic pathogens can foster an autoimmune-prone environment and drive systemic inflammation leading to endothelial barrier defects. in mouse model cohorts, we demonstrate that infection with the protozoan parasite, toxoplasma gondii, caused sustained elevations of igg class antibodies to the nmdar in conjunction ... | 2017 | 28763062 |
| seroepidemiological study of toxoplasma gondii infection among psychiatric patients in mashhad, northeast of iran. | psychiatric patients have an increased risk of some infections like toxoplasmosis. investigations on toxoplasma gondii infection among psychiatric patients have been limited in mashhad, northeast of iran. in this case-control study, prevalence of t. gondii was investigated by serological method. | 2017 | 28761468 |
| molecular detection and genotyping of toxoplasma gondii in chicken, beef, and lamb meat consumed in northwestern iran. | toxoplasma gondii, the obligate, intracellular parasite afflicts human in diverse ways such as ingestion of tissue cysts in undercooked meat. the aim of this study was to assess the prevalence and genotyping of toxoplasma isolated from meat samples being consumed in east azerbaijan, northwest of iran. | 2017 | 28761459 |
| down-modulation of effector functions in nk cells upon t. gondii infection. | the obligate intracellular parasite toxoplasma gondii (t. gondii) can actively infect any nucleated cell type including cells from the immune system. rapid transfer of t. gondii from infected dendritic cells to effector natural killer (nk) cells may contribute to the parasite's sequestration and shielding from immune recognition shortly after infection. however, subversion of nk cell functions, such as cytotoxicity or production of pro-inflammatory cytokines such as ifnγ, upon parasite infection ... | 2017 | 28760930 |
| toxoplasma gondii, a foodborne pathogen in the swine production chain from a european perspective. | toxoplasmosis is a foodborne zoonosis transmitted by toxoplasma gondii, a cosmopolitan protozoan that infects humans through exposure to different parasite stages, in particular by ingestion of tissue cysts or tachyzoites contained in meat, primary offal (viscera), and meat-derived products or ingestion of environmental sporulated oocysts in contaminated food or water. the pig is an important species for infection: raw or undercooked pork consumption not subject to treatment able to inactivate t ... | 2017 | 28759251 |
| genetic diversity of toxoplasma gondii isolates from ruminants: a systematic review. | toxoplasma gondii is a protozoan capable of infecting all warm-blooded animals. this parasite has been classified into three major lineages. our aim was to assess and compare the identified types and genotypes in ruminants. from november 2014 to april 2015, four english language databases and four persian databases that reported data on the t. gondii genotyping in ruminants were searched. overall, typing results of the 250/307 t. gondii isolates in all animals showed that type ii was a predomina ... | 2017 | 28756372 |
| anti-toxoplasma gondii antibodies in beef cattle slaughtered in the metropolitan region of belém, brazilian amazon. | the relevance of consuming raw or undercooked beef in the transmission of toxoplasmosis is unclear due to the high resistance of cattle to infection. however, this possibility needs to be considered in endemic areas, such as the amazon, where the consumption of beef is frequent. the objective of this study was to determine the frequency of anti-toxoplasma gondii igg antibodies in beef cattle slaughtered in the metropolitan region of belem, pará state, brazil. blood samples were collected from 50 ... | 2017 | 28746450 |
| seroprevalence of anti-toxoplasma gondii antibodies in healthy voluntary blood donors from mashhad city, iran. | the present investigation aims to evaluate the prevalence of igm and igg anti-t. gondii antibodies and the associated risk factors among healthy blood donors in mashhad city, razavi khorasan province, iran. | 2017 | 28745905 |
| nadph oxidase 4 is required for the generation of macrophage migration inhibitory factor and host defense against toxoplasma gondii infection. | nicotinamide adenine dinucleotide phosphate (nadph) oxidases (nox) are an important family of catalytic enzymes that generate reactive oxygen species (ros), which mediate the regulation of diverse cellular functions. although phagocyte nox2/gp91phox is closely associated with the activation of host innate immune responses, the roles of nox family protein during toxoplasma gondii (t. gondii) infection have not been fully investigated. here, we found that t. gondii-mediated ros production was requ ... | 2017 | 28743960 |
| aid-ing signaling in toxoplasma gondii. | the cyclic gmp-dependent protein kinase (pkg) of apicomplexan parasites is essential for secretion of micronemes and host cell invasion and egress. both kinase specificity and localization can determine which substrates are phosphorylated. the functions of plasma membrane and cytosolic pkg isoforms of toxoplasma gondii were unknown because of difficulties precisely manipulating expression of essential genes. brown et al. (k. m. brown, s. long, and l. d. sibley, mbio 8:e00375-17, https://doi.org/ ... | 2017 | 28743818 |
| inherent oxidative stress in lewis rat is associated with resistance to toxoplasmosis. | the course of toxoplasma gondii infection in rats closely resembles that in humans. however, compared to the brown norway (bn) rat, the lewis (lew) rat is extremely resistant to t. gondii infection. thus, we performed rna sequencing analysis of the lew versus bn rat, with or without t. gondii infection, in order to unravel molecular factors directing robust and rapid early t. gondii-killing mechanisms in the lew rat. we found that, compared to the uninfected bn rat, the uninfected lew rat has in ... | 2017 | 28739829 |
| ovine toxoplasmosis: a new look at its pathogenesis. | conditions causing reproductive failure are a significant concern in the livestock industry, and amongst these, ovine toxoplasmosis is one of the most important. despite decades of research, there remain gaps in knowledge about this disease, especially regarding the pathogenesis of abortion in sheep. as for other diseases causing ovine abortion, such as chlamydial abortion or border disease, the consequences of infection with toxoplasma gondii depend largely on the stage of gestation, but the me ... | 2017 | 28735668 |
| association of toxoplasma gondii infection with schizophrenia and its relationship with suicide attempts in these patients. | to investigate the association between schizophrenia and toxoplasma gondii, and to assess the association of infection with suicide attempts and age of onset of schizophrenia in these patients. | 2017 | 28734096 |
| spatial and simultaneous representative seroprevalence of anti-toxoplasma gondii antibodies in owners and their domiciled dogs in a major city of southern brazil. | toxoplasmosis, caused by toxoplasma gondii, has traditionally been considered an important water and foodborne protozoonosis with important public health considerations. although felids play a well-established role as definitive hosts, canine epidemiological involvement in the parasite's life cycle remains questionable and controversial. the increasing closeness of the human-dog bond, particularly seen in urban settings, has been recognized as a historically unprecedented worldwide movement. sha ... | 2017 | 28732033 |
| toxoplasma gondii: entry, association, and physiological influence on the central nervous system. | toxoplasma gondii is one of the world's most successful parasites, in part because of its ability to infect and persist in most warm-blooded animals. a unique characteristic of t. gondii is its ability to persist in the central nervous system (cns) of a variety of hosts, including humans and rodents. how, what, and why t. gondii encysts in the cns has been the topic of study for decades. in this review, we will discuss recent work on how t. gondii is able to traverse the unique barrier surroundi ... | 2017 | 28727854 |
| a plant/fungal-type phosphoenolpyruvate carboxykinase located in the parasite mitochondrion ensures glucose-independent survival of toxoplasma gondii. | toxoplasma gondii is considered as one of the most successful intracellular pathogens, because it can reproduce in varied nutritional milieus, encountered in diverse host-cell types of essentially any warm-blooded organism. our earlier work has demonstrated that the acute (tachyzoite) stage of t. gondii depends on cooperativity of glucose and glutamine catabolism to meet biosynthetic demands. either of these two nutrients can sustain the parasite survival; however, what determines the metabolic ... | 2017 | 28726641 |
| adhesion of toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression. | intracellular pathogens have numerous strategies for effective dissemination within the host. many intracellular pathogens first infect leukocytes, which they use as a vehicle to transport them to target organs. once at the target organ, intracellular parasite toxoplasma gondii can cross the capillary wall in extracellular form by infecting endothelial cells. however, after egression from leukocytes, extracellular parasites face the risk of host immune attack. in this study, observation of infec ... | 2017 | 28720868 |
| profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis. | toxoplasma gondii, a common opportunistic protozoan, is a leading cause of illness and mortality among immunosuppressed individuals and during congenital infections. current therapeutic strategies for toxoplasmosis are not fully effective at curtailing disease progression in these cases. given the parasite ability to influence host immunity and metabolism, understanding of the metabolic alterations in the host's immune organs during t. gondii infection may enhance the understanding of the molecu ... | 2017 | 28720125 |
| seroprevalence of toxoplasmosis with elisa and rapid diagnostic test among residents in gyodong-do, inchon city, korea: a four-year follow-up. | elisa has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (rdt). we compared and analyzed elisa and rdt results using the sera collected during 4 consecutive years from residents of gyodong-do (island), incheon-city, korea. sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). elisa was performed by using a crude extract of t. gondii rh strain antigen and igg/igm rdt mounted with recombinant frag ... | 2017 | 28719949 |
| isolation and genotyping of toxoplasma gondii in brazilian dogs. | strains of toxoplasma gondii in brazil are highly genetically diverse compared to strains from north america and europe. dogs are epidemiologically important because they act as sentinels for t. gondii infections in humans and are good indicators of environmental contamination. the aim of this study was to isolate and genetically characterize t. gondii strains from tissues of naturally infected brazilian dogs. for this study, 21 blood samples were collected from dogs at the zoonosis control cent ... | 2017 | 28719948 |
| phosphoproteome of toxoplasma gondii infected host cells reveals specific cellular processes predominating in different phases of infection. | the invasion of toxoplasma gondii tachyzoites into the host cell results in extensive host cell signaling activation/deactivation that is usually regulated by the phosphorylation/dephosphorylation. to elucidate how t. gondii regulates host cell signal transduction, the comparative phosphoproteome of stable isotope labeling with amino acids in cell culture-labeled human foreskin fibroblast cells was analyzed. the cells were grouped (light [l], medium [m], and heavy [h] groups) based on the labeli ... | 2017 | 28719319 |
| seroprevalence of toxoplasma gondii infection in refugee and migrant pregnant women along the thailand-myanmar border. | toxoplasma gondii primary infection in pregnancy is associated with poor obstetric outcomes. this study aimed to determine the seroprevalence of toxoplasma infection in pregnant migrant and refugee women from myanmar attending antenatal care in thailand. a random selection of 199 residual blood samples from first antenatal screen in 2014-2015 was tested for toxoplasma igg and igm antibodies. seroprevalence of toxoplasma infection was 31.7% (95% confidence interval = 25.6-38.4). avidity testing i ... | 2017 | 28719309 |
| evaluation of the protective effect of deoxyribonucleic acid vaccines encoding granule antigen 2 and 5 against acute toxoplasmosis in balb/c mice. | abstracttoxoplasma gondii infects a broad range of warm-blooded hosts, including humans. important clinical manifestations include encephalitis in immunocompromised patients as well as miscarriage and fetal damage during early pregnancy. toxoplasma gondii dense granule antigen 2 and 5 (gra2 and gra5) are essential for parasitophorous vacuole development of the parasite. to evaluate the potential of gra2 and gra5 as recombinant dna vaccine candidates, these antigens were cloned into eukaryotic ex ... | 2017 | 28719288 |
| toxoplasma gondii infection and common mental disorders in the finnish general population. | we investigated whether t. gondii seropositivity is associated with 12-month depressive, anxiety and alcohol use disorders and current depressive symptoms and whether inflammation, measured by c-reactive protein (crp) level, explains these associations. | 2017 | 28715724 |
| diarrhea as a presenting symptom of disseminated toxoplasmosis. | disseminated toxoplasmosis is uncommon in both immunocompetent and immunocompromised hosts with gastrointestinal involvement being rarely described. we report a case of disseminated gastrointestinal toxoplasmosis in an immunocompromised man who presented with one month of diarrhea and abdominal pain. imaging showed thickening of the ascending colon and cecum. esophagogastroduodenoscopy and colonoscopy biopsies revealed toxoplasma gondii, confirmed by immunostain. symptoms completely resolved fol ... | 2017 | 28713605 |
| the association between toxoplasma and the psychosis continuum in a general population setting. | toxoplasma gondii infection is associated with increased risk for psychosis. however, the possible association between t. gondii and psychotic-like symptoms in the general adult population is unknown. we investigated whether t. gondii is associated with psychotic-like symptoms and psychosis diagnoses using data from health 2000, a large cross-sectional health survey of the finnish general population aged 30 and above. seropositivity to toxoplasma was defined as a cutoff of 50iu/ml of igg antibod ... | 2017 | 28711477 |
| molecular detection of toxoplasma gondii infection in slaughtered ruminants (sheep, goats and cattle) in northwest tunisia. | the present study aimed to estimate the molecular prevalence of t. gondii infection in meat from slaughtered sheep, goats and cattle in northwest tunisia (béja district). pcrs were performed on genomic dna extracted from 420 meat samples (150 ewes, 120 goats and 150 cows). the overall molecular prevalence of t. gondii in sheep, goats and cattle were 33.3 (50/150), 32.5 (39/120) and 19.3% (29/150), respectively. toxoplasma gondii molecular prevalences in the three meat ruminant species were signi ... | 2017 | 28711018 |
| first detection and genetic characterization of toxoplasma gondii in market-sold oysters in china. | toxoplasma gondii oocysts in the water might be filtered through the gills of shellfish in the process of feeding, and can be concentrated in the digestive glands. consumers might become infected through ingestion of the shellfish unless they are discharged or inactivated by the shellfish. thus, the purpose of this study was to evaluate the presence of t. gondii in market-sold oysters in china under natural conditions using a molecular approach. a total of 998 oysters were collected from markets ... | 2017 | 28710018 |
| toxoplasmosis treatment with diphenyl diselenide in infected mice modulates the activity of purinergic enzymes and reduces inflammation in spleen. | toxoplasma gondii, an intracellular protozoan, may cause chronic infection in the brain tissue of the host inducing a systemic pro-inflammatory profile. chronic infections can induce numerous physiological changes, such as alterations in the immune and oxidative profiles. diphenyl diselenide (phse)2, an organoselenium compound, has shown antioxidant and immunomodulatory activities in recent studies. so, the aim of this study was to investigate the activity of purinergic enzymes and reactive oxyg ... | 2017 | 28710007 |
| impact of the host on toxoplasma stage differentiation. | the unicellular parasite toxoplasma gondii infects warm-blooded animals and humans, and it is highly prevalent throughout the world. infection of immunocompetent hosts is usually asymptomatic or benign but leads to long-term parasite persistence mainly within neural and muscular tissues. the transition from acute primary infection towards chronic toxoplasmosis is accompanied by a developmental switch from fast replicating and metabolically highly active tachyzoites to slow replicating and largel ... | 2017 | 28706936 |
| immunization with toxoplasma gondii gra17 deletion mutant induces partial protection and survival in challenged mice. | toxoplasmosis remains a world-threatening disease largely because of the lack of a fully effective vaccine. here, we created a δgra17 mutant by disrupting the virulence factor gra17 using crispr-cas9 method. then, we tested whether δgra17 tachyzoites can be used as a live-attenuated vaccine against acute, chronic, and congenital toxoplasma gondii infection in mice. immune response evoked by δgra17 immunization suggested a sequential th1 and th2 t cell response, indicated by high levels of th1 an ... | 2017 | 28706518 |
| trim21 is critical for survival of toxoplasma gondii infection and localises to gbp-positive parasite vacuoles. | interferon gamma (ifnγ) is the major proinflammatory cytokine conferring resistance to the intracellular vacuolar pathogen toxoplasma gondii by inducing the destruction of the parasitophorous vacuole (pv). we previously identified trim21 as an ifnγ-driven e3 ubiquitin ligase mediating the deposition of ubiquitin around pathogen inclusions. here, we show that trim21 knockout mice were highly susceptible to toxoplasma infection, exhibiting decreased levels of serum inflammatory cytokines and highe ... | 2017 | 28701773 |
| the first detection of toxoplasma gondii dna in environmental air samples using gelatine filters, real-time pcr and loop-mediated isothermal (lamp) assays: qualitative and quantitative analysis. | toxoplasma gondii infections are acquired through the ingestion of oocysts present in the environment. however, there is no data about their occurrence in the air or about airborne transmission of these infections. in the present paper, we report on the identification of t. gondii using rapid molecular detection methods, supported by microscopic analysis, in environmental air samples. a total of 71 samples were collected, using gelatine filters, from kitchen gardens, recreational areas and sandp ... | 2017 | 28697821 |
| association of parasite load levels in amniotic fluid with clinical outcome in congenital toxoplasmosis. | to correlate neonatal and infant clinical outcome with parasite load in amniotic fluid (af). | 2017 | 28697120 |
| in vitro activity of the interaction between taxifolin (dihydroquercetin) and pyrimethamine against toxoplasma gondii. | toxoplasmosis is one of the most neglected zoonotic foodborne parasitic diseases that cause public health and socioeconomic concern worldwide. the current drugs used for the treatment of toxoplasmosis have been identified to have clinical limitations. hence, new drugs are urgently needed to eradicate t.gondii infections globally. here, an in vitro anti-toxoplasma gondii activity of taxifolin (dihydroquercetin) and dihydrofolate inhibitor (pyrimethamine) alone and in combination with a fixed conc ... | 2017 | 28696589 |
| a more sensitive, efficient and iso 17025 validated magnetic capture real time pcr method for the detection of archetypal toxoplasma gondii strains in meat. | toxoplasma gondii is a globally prevalent, zoonotic parasite of major importance to public health. various indirect and direct methods can be used for the diagnosis of toxoplasmosis. whereas serological tests are useful to prove contact with the parasite has occurred, the actual presence of the parasite in the tissues of a seropositive animal is not demonstrated. for this, a bioassay is still the reference method. as an alternative, various pcr methods have been developed, but due to the limited ... | 2017 | 28694187 |
| the standardization of 5 immunoassays for anti-toxoplasma immunoglobulin g(igg). | quantitative immunoassays to detect igg antibodies are the most commonly used tests for diagnosing toxoplasmosis. we investigated the current state of standardization of quantitative immunoassays used to measure anti-toxoplasma igg levels. | 2017 | 28694124 |
| seroprevalence and risk factors of toxoplasma gondii infection in pregnant women from bobo dioulasso, burkina faso. | toxoplasmosis is one of the common worldwide parasitic zoonosis due to toxoplasma gondii (t. gondii). toxoplasmosis during pregnancy can result in fetal and neonatal death or various congenital defects. the aim of this study was to assess the seroprevalence and risk factors of t. gondii infection in pregnant women following antenatal care (anc) services at bobo dioulasso. | 2017 | 28693432 |
| congenital toxoplasmosis in austria: prenatal screening for prevention is cost-saving. | primary infection of toxoplasma gondii during pregnancy can be transmitted to the unborn child and may have serious consequences, including retinochoroiditis, hydrocephaly, cerebral calcifications, encephalitis, splenomegaly, hearing loss, blindness, and death. austria, a country with moderate seroprevalence, instituted mandatory prenatal screening for toxoplasma infection to minimize the effects of congenital transmission. this work compares the societal costs of congenital toxoplasmosis under ... | 2017 | 28692640 |
| evaluation of serological and molecular tests used to identify toxoplasma gondii infection in pregnant women attended in a public health service in são paulo state, brazil. | toxoplasmosis during pregnancy can have severe consequences. the use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. we compared the elisa and elfa serological methods, conventional pcr (cpcr), nested pcr and quantitative pcr (qpcr) in the diagnosis of toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (g1=94) and with clinical suspicion of toxoplasmosis (g2=53). the results were c ... | 2017 | 28689893 |
| sequence variation in the b1 gene among toxoplasma gondii isolates from swine and cats in italy. | the evaluation of the genetic variations of toxoplasma gondii among isolates of a wide variety of animal hosts can provide significant information for better understanding the epidemiology and population structure of the parasite in different geographical areas. the aim of this study was to provide information on t. gondii genetic diversity in host species living in central italy, which could act as a potential source of human infection. seventy-seven feline faecal samples, and 36 and 20 diaphra ... | 2017 | 28689144 |
| toxoplasmosis accelerates acquisition of epilepsy in rats undergoing chemical kindling. | epilepsy is one of the most common neurologic disorders worldwide with no distinguishable cause in 60% of patients. one-third of the world population has been infected with toxoplasma gondii. this intracellular parasite has high tropism for excitable cells including neurons. we assessed impact of acute and chronic t. gondii infection on epileptogenesis in pentylenetetrazole (ptz) kindling model in male rats. t. gondii cysts were administered to rats by intraperitoneal (i.p.) injection. the prese ... | 2017 | 28688333 |
| actin nanobodies uncover the mystery of actin filament dynamics in toxoplasma gondii. | while the intracellular parasite toxoplasma relies on a divergent actomyosin motor to support unique speeds in directional movement, the dynamics and architecture of parasite actin filaments remain a much-discussed issue. using actin chromobodies, periz et al. started to unveil how networks of dynamic f-actin connect toxoplasma progeny and expand in the replicative vacuole. | 2017 | 28687476 |
| first toxoplasma gondii isolate from an aborted foetus of european bison (bison bonasus bonasus l.). | the study was performed on a male european bison (bison bonasus bonasus l.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the białowieża forest. serum samples from the foetus and mother revealed the presence of antibodies against t. gondii (s/p% = 88% and 75%, respectively). mobile extracellular tachyzoites were first observed in a vero cell culture, 110 days following inoculation of brain homogenate. pcr amplification with tgr1e1 and tgr1e2 primers confirmed the pres ... | 2017 | 28685180 |
| seroprevalence and risk factors of neospora spp. and toxoplasma gondii infections among horses and donkeys in nigeria, west africa. | neospora spp. and toxoplasma gondii are considered to be a globally distributed parasites affecting wide range of warm-blooded animals. neosporosis has caused clinical illness in horses and consumption of horse meat has been epidemiologically linked to clinical toxoplasmosis in humans. this study was conducted to determine neospora spp. and t. gondii antibodies and risk factors of infection in horses and donkeys from three states of nigeria. a total of 144 samples were collected from clinically ... | 2017 | 28682771 |
| epidemiology of toxoplasma gondii infection in native tibetans in tibet, china. | toxoplasma gondii (t. gondii) is one of the most common and widespread protozoan parasite which caused toxoplasma infection in animals and humans. toxoplasma infection in healthy people is usually asymptomatic and it can lead to serious pathological effects in congenital cases and immunodeficient patients. the aim of the study is to investigate the prevalence of t. gondii infection in native tibetans in tibet as scarce information is available on such a high plateau. a total of 600 blood samples ... | 2017 | 28682769 |
| structuraland antigenic analysis of a new rhoptry pseudokinase gene (rop54) in toxoplasma gondii. | toxoplasma gondii is defined as an obligate intracellular apicomplexan parasite and influences approximatelyone-third of the human all over the world. rop54 protein is expressed in the rhoptry of toxoplasma gondii. in the present study, we used smart software to analyzethe secondary structure of rop54. the 3d model of rop54 protein was constructed and analyzed using swiss-model server and vmd software. the structure results fully showed that rop54 proteinis an importantmember from the rop family ... | 2017 | 28682759 |
| behavior of neutrophil granulocytes during toxoplasma gondii infection in the central nervous system. | cerebral toxoplasmosis is characterized by activation of brain resident cells and recruitment of specific immune cell subsets from the periphery to the central nervous system (cns). our studies revealed that the rapidly invaded ly6g(+) neutrophil granulocytes are an early non-lymphoid source of interferon-gamma (ifn-γ), the cytokine known to be the major mediator of host resistance to toxoplasma gondii (t. gondii). upon selective depletion of ly6g(+) neutrophils, we detected reduced ifn-γ produc ... | 2017 | 28680853 |
| seroepidemiology of toxoplasma gondii infection in patients with liver disease in eastern china. | the role of the protozoan parasite toxoplasma gondii in the pathogenesis of liver disease has recently gained much interest. the aim of this study was to determine the prevalence and risk factors associated with t. gondii infection in patients with liver disease from three cities in shandong and henan provinces, china. a case-control study was conducted from december 2014 to november 2015 and included 1142 patients with liver disease and 1142 healthy controls. serum samples were collected from a ... | 2017 | 28677516 |
| report of an unsual case of anophthalmia and craniofacial cleft in a newborn with toxoplasma gondii congenital infection. | we present one unusual case of anophthalmia and craniofacial cleft, probably due to congenital toxoplasmosis only. | 2017 | 28673238 |
| checkpoints of apicomplexan cell division identified in toxoplasma gondii. | the unusual cell cycles of apicomplexa parasites are remarkably flexible with the ability to complete cytokinesis and karyokinesis coordinately or postpone cytokinesis for several rounds of chromosome replication, and are well recognized. despite this surprising biology, the molecular machinery required to achieve this flexibility is largely unknown. in this study, we provide comprehensive experimental evidence that apicomplexan parasites utilize multiple cdk-related kinases (crks) to coordinate ... | 2017 | 28671988 |
| qtl mapping and crispr/cas9 editing to identify a drug resistance gene in toxoplasma gondii. | scientific knowledge is intrinsically linked to available technologies and methods. this article will present two methods that allowed for the identification and verification of a drug resistance gene in the apicomplexan parasite toxoplasma gondii, the method of quantitative trait locus (qtl) mapping using a whole genome sequence (wgs) -based genetic map and the method of clustered regularly interspaced short palindromic repeats (crispr)/cas9 -based gene editing. the approach of qtl mapping allo ... | 2017 | 28671645 |
| prevalence of toxoplasma gondii igg and igm and associated risk factors among hiv-positive and hiv-negative patients in vhembe district of south africa. | toxoplasma gondii is a zoonotic parasite that has arisen as an important opportunistic infection that causes morbidity and mortality especially in hiv positive patients. this study was carried out to determine the sero-prevalence of t. gondii (igg and igm) and the associated risk factors among hiv positive and negative patients in northern south africa. | 2017 | 28670634 |
| role of nets in the difference in host susceptibility to toxoplasma gondii between sheep and cattle. | the main aim of this study was to compare extracellular traps (nets) formation by polymorphonuclear neutrophils (pmns) of cattle and sheep when exposed to t. gondii tachyzoites in vitro. the effects of parasite concentrations and different incubation periods on nets development in cattle and sheep pmns were studied. the effect of net structures on host cell invasion by tachyzoites was also studied. this is the first report of nets development by sheep and cattle pmns against t. gondii in vitro. ... | 2017 | 28669381 |
| toxoplasma genotyping in congenital toxoplasmosis in upper egypt: evidence of type i strain. | toxoplasma gondii has subpopulation structures in different geographical regions caused by less frequent sexual recombination, population sweeps, and biogeography. the majority of strains isolated in north america and europe fall into one of three clonal lineages, referred to as types i, ii, and iii. so far, little is known about genetics of toxoplasma strains in africa. the present study aimed to determine the genotype of toxoplasma strains obtained directly from trophoblastic/placental tissues ... | 2017 | 28668985 |
| the toxoplasma gondii inhibitor-2 regulates protein phosphatase 1 activity through multiple motifs. | toxoplasma gondii has a complex life cycle characterized by multiple differentiation steps that are essential for its survival in both human and definitive feline host. several studies have demonstrated the importance of phosphorylations by protein kinases during the life cycle of t. gondii. however, very little is known about protein phosphatases and their regulators in the parasite. we report the molecular and functional characterization of the t. gondii ortholog of the inhibitor-2 protein, de ... | 2017 | 28667522 |
| cerebral toxoplasmosis after haematopoietic stem cell transplantation. | toxoplasmosis is an opportunistic infection caused by the parasite toxoplasma gondii. the infection is severe and difficult to diagnose in patients receiving allogeneic haematopoietic stem cell transplantation (hsct). it frequently involves the central nervous system. the case is presented of cerebral toxoplasmosis in a 17-year-old youth with fanconi anaemia treated with haematopoietic stem cell transplantation (hsct). | 2017 | 28664700 |
| the moving junction protein ron4, although not critical, facilitates host cell invasion and stabilizes mj members. | toxoplasma gondii is an obligate intracellular parasite of phylum apicomplexa. to facilitate high-efficiency invasion of host cells, t. gondii secretes various proteins related to the moving junction (mj) complex from rhoptries and micronemes into the interface between the parasite and host. ama1/ron2/4/5/8 is an important mj complex, but its mechanism of assembly remains unclear. in this study, we used the crispr-cas9 system to generate a derivative of t. gondii strain rh with a null mutation i ... | 2017 | 28662729 |
| risk factors associated with toxoplasma gondii infection in free-range chickens in the semiarid region of brazil. | this study aimed to investigate the frequency of anti-toxoplasma gondii antibodies in serum from 629 chickens on 39 family farms in seven municipalities in the semiarid region, pernambuco, brazil, and to identify risk factors associated with t. gondii infection. the risk factors were studied in 421 samples from 29 farms. anti-t. gondii antibodies were investigated by indirect fluorescent antibody test with a 1:16 cutoff. the frequency of positive chickens was 27.9% (176/629) and 94.8% of the far ... | 2017 | 28658418 |
| a plastid protein that evolved from ubiquitin and is required for apicoplast protein import in toxoplasma gondii. | apicomplexan parasites cause a variety of important infectious diseases, including malaria, toxoplasma encephalitis, and severe diarrhea due to cryptosporidium most apicomplexans depend on an organelle called the apicoplast which is derived from a red algal endosymbiont. the apicoplast is essential for the parasite as the compartment of fatty acid, heme, and isoprenoid biosynthesis. the majority of the approximate 500 apicoplast proteins are nucleus encoded and have to be imported across the fou ... | 2017 | 28655825 |
| mechanism of dexamethasone in the context of toxoplasma gondii infection. | toxoplasmosis is a serious zoonoses disease and opportunistic, and can be life-threatening. dexamethasone (dex) is widely used in the clinic for treatment of inflammatory and autoimmune diseases. however, long-term use of dex is often easy to lead to acute toxoplasmosis in patients, and the potential molecular mechanism is still not very clear. the aims of this study were to investigate the effect of dex on proliferation of toxoplasma and its molecular mechanisms, and to establish the correspond ... | 2017 | 28653591 |
| east-west divide: temperature and land cover drive spatial variation of toxoplasma gondii infection in eurasian otters (lutra lutra) from england and wales. | toxoplasma gondii, a zoonotic parasite of global importance, infects all endothermic vertebrates, with extensive health implications. the prevalence of this parasite is seldom monitored in wildlife. here, a semi-aquatic species, the eurasian otter (lutra lutra) was used as a model to assess the potential effect of climate, land cover and biotic factors on t. gondii seroprevalence in british wildlife. the sabin-feldman cytoplasm-modifying dye test identified t. gondii antibodies in 25·5% of blood ... | 2017 | 28653587 |
| characterization of the activities of dinuclear thiolato-bridged arene ruthenium complexes against toxoplasma gondii. | the in vitro effects of 18 dinuclear thiolato-bridged arene ruthenium complexes (1 monohiolato compound, 4 dithiolato compounds, and 13 trithiolato compounds), originally designed as anticancer agents, on the apicomplexan parasite toxoplasma gondii grown in human foreskin fibroblast (hff) host cells were studied. some trithiolato compounds exhibited antiparasitic efficacy at concentrations of 250 nm and below. among those, complex 1 and complex 2 inhibited t. gondii proliferation with 50% inhibi ... | 2017 | 28652238 |
| point-of-care testing for toxoplasma gondii igg/igm using toxoplasma ict igg-igm test with sera from the united states and implications for developing countries. | congenital toxoplasmosis is a serious but preventable and treatable disease. gestational screening facilitates early detection and treatment of primary acquisition. thus, fetal infection can be promptly diagnosed and treated and outcomes can be improved. | 2017 | 28650970 |
| immunization of c57bl/6 mice with gra2 combined with mpl conferred partial immune protection against toxoplasma gondii | we have previously reported that immunization with gra2 antigen of toxoplasma gondii induces protective immunity in cba/j (h2k) and balb/c mice (h2d). we aimed to examine whether immunization of a distinct strain of rodent with recombinant dense granule antigens (gra2) combined with monophosphorryl lipid a (mpl) adjuvant elicits protective immune response against t. gondii. | 2017 | 28646827 |
| proteomic profiling of extracellular vesicles secreted from toxoplasma gondii. | toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. recently, exosomes, small extracellular vesicles (ev) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. the effect of evs derived from t. gondii on the immune response and its relevance in a physiological context is unknown. here we disclose the first proteomic profiling of t. gondii evs compared ... | 2017 | 28643940 |
| strain- and dose-dependent reduction of toxoplasma gondii burden in pigs is associated with interferon-gamma production by cd8(+) lymphocytes in a heterologous challenge model. | toxoplasma gondii is a worldwide prevalent parasite of humans and animals. the global infection burden exceeds yearly one million disability-adjusted life years (daly's) in infected individuals. therefore, effective preventive measures should be taken to decrease the risk of infection in humans. although human toxoplasmosis is predominantly foodborne by ingestion of tissue cysts in meat from domestic animals such as pigs, the incidence risk is difficult to estimate due to the lack of screening o ... | 2017 | 28642841 |
| is toxoplasma gondii infection protective against multiple sclerosis risk? | parasitic infections may play a protective role in neurodegenative diseases. objectıve: to determine the association between toxoplasma gondii (t. gondii) infection and multiple sclerosis (ms). | 2017 | 28641775 |
| survival of an aids patient after infection with acanthamoeba sp. of the central nervous system. | a 38-year-old man presented with headaches and generalized weakness. he was found to have aids; a ring-enhancing central nervous system lesion was found on brain imaging and he had elevated serum toxoplasma gondii igg levels. a diagnosis of presumptive toxoplasma encephalitis was made and he received antiretrovirals and antitoxoplasma therapy for 4 years. intermittent headaches and evidence of disease progression on neuroimaging warranted further evaluation and cerebrospinal fluid analysis revea ... | 2017 | 28639141 |
| first report of typical brazilian toxoplasma gondii genotypes from isolates of free-range chickens (gallus gallus domesticus) circulating in the state of paraíba, northeast brazil. | this study evaluated, for the first time, the genetic diversity of toxoplasma gondii isolates from free-range chickens from the state of paraíba, northeast brazil. tissue samples from 33 chickens from properties in five municipalities of paraíba (esperança, olho d'água, malta, monteiro, and patos) were bioassayed in mice. the brains of mice infected with t. gondii cysts were used for dna extraction and genotyping. genotyping was performed using 11 pcr-rflp markers and 15 microsatellite (ms) mark ... | 2017 | 28638980 |
| infectious disease surveillance in the woylie (bettongia penicillata). | wild populations of the critically endangered woylie (bettongia penicillata) recently declined by 90% in southwest western australia. increased predation is the leading hypothesis for decline, but disease may be playing a role increasing susceptibility to predation. to explore this possibility, we surveyed woylie populations in the wild, in captivity and in a predator-free sanctuary for exposure to, and infection with, four known pathogens of macropods: herpesviruses, wallal and warrego orbiviru ... | 2017 | 28634781 |
| development of neurological mouse model for toxoplasmosis using toxoplasma gondii isolated from chicken in kenya. | animal models for the toxoplasmosis are scarce and have limitations. in this study, a neurological mouse model was developed in balb/c mice infected intraperitoneally with 15 cysts of a toxoplasma gondii isolate. the mice were monitored for 42 days and euthanized at different time points. another group of mice were orally treated with dexamethasone (dxm: 2.66 mg/kg daily, 5.32 mg/kg daily) at 42 days after infection and monitored for a further 42 days. a mortality rate of 15% and 28.6% was obser ... | 2017 | 28630781 |
| ccr5 is involved in interruption of pregnancy in mice infected with toxoplasma gondii during early pregnancy. | toxoplasmosis can cause abortion in pregnant humans and other animals; however, the mechanism of abortion remains unknown. c-c chemokine receptor type 5 (ccr5) is essential for host defense against toxoplasma gondii infection. to investigate the relationship between ccr5 and abortion in toxoplasmosis, we inoculated wild-type and ccr5-deficient (ccr5(-/-)) mice with t. gondii tachyzoites intraperitoneally on day 3 of pregnancy (embryonic day 3 [e3]). the pregnancy rate decreased as pregnancy prog ... | 2017 | 28630065 |
| association between toxoplasma gondii types and outcomes of human infection: a meta-analysis. | the virulence and pathogenicity of various types of toxoplasma gondii differ considerably in mice. recent studies have claimed that similar phenomenon was observed in humans, but no relevant studies have been performed to validate this finding. in addition, reports showing association between a given t. gondii type and outcomes of human infection yielded conflicting results. to provide a more precise estimation of the association and a more reliable conclusion on this subject, we performed this ... | 2017 | 28629230 |
| igg avidity test in congenital toxoplasmosis diagnoses in newborns. | the goal of this study was to investigate the importance of igg avidity testing in newborns (nbs) diagnosed with early congenital toxoplasmosis. we collected samples from 88 puerperae infected by toxoplasma gondii (t. gondii) and their nbs (48 acutely-infected puerperae (aip) and 40 chronically-infected puerperae (cip)), from two public maternity hospitals in goiania city, goias, brazil, from 2010 to 2015. specific anti-t. gondii igm and igg serum levels and igg avidity tests were evaluated usin ... | 2017 | 28629167 |
| toxoplasma depends on lysosomal consumption of autophagosomes for persistent infection. | globally, nearly 2 billion people are infected with the intracellular protozoan toxoplasma gondii(1). this persistent infection can cause severe disease in immunocompromised people and is epidemiologically linked to major mental illnesses(2) and cognitive impairment(3). there are currently no options for curing this infection. the lack of effective therapeutics is due partly to a poor understanding of the essential pathways that maintain long-term infection. although it is known that toxoplasma ... | 2017 | 28628099 |
| the heat shock protein 90 of toxoplasma gondii is essential for invasion of host cells and tachyzoite growth. | toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects almost all warm-blooded vertebrates. heat shock proteins (hsp) regulate key signal transduction events in many organisms, and heat shock protein 90 (hsp90) plays an important role in growth, development, and virulence in several parasitic protozoa. here, we discovered increased transcription of the hsp90 gene under conditions for bradyzoite differentiation, i.e. alkaline and heat shock conditions in vitro, suggesti ... | 2017 | 28627357 |
| proteomic differences between developmental stages of toxoplasma gondii revealed by itraq-based quantitative proteomics. | toxoplasma gondii has a complex two-host life-cycle between intermediate host and definitive host. understanding proteomic variations across the life-cycle stages of t. gondii may improve the understanding of molecular adaption mechanism of t. gondii across life-cycle stages, and should have implications for the development of new treatment and prevention interventions against t. gondii infection. here, we utilized lc-ms/ms coupled with itraq labeling technology to identify differentially expres ... | 2017 | 28626452 |
| evaluation of protective immunity induced by dna vaccination with genes encoding toxoplasma gondii gra17 and gra23 against acute toxoplasmosis in mice. | toxoplasma gondii, an obligatory intracellular protozoan, can cause serious public health problems and economic losses worldwide. two novel dense granule proteins (gra17 and gra23) were recently identified as t. gondii-secreted proteins which are localized to the parasitophorous vacuole membrane (pvm) and can mediate the movement of small molecules between the host cell and parasitophorous vacuole (pv). in the present study, we evaluated the protective immunity induced by dna vaccination with ge ... | 2017 | 28625894 |
| schizogony and gametogony of oocyst-deficient t-263 strain of toxoplasma gondii. | oocysts are important stage for the spread of toxoplasma gondii because they are environmentally resistant. among all hosts of t. gondii, only felids can excrete oocysts. cats that have excreted t. gondii oocysts after primary infection develop immunity to re-excretion of oocysts, and this immunity appears to be long-lasting. it would be desirable to have a non-infectious vaccine for the prevention of t. gondii infection in cats and to understand mechanism of immunity to excretion of oocysts. an ... | 2017 | 28624132 |
| pcr-rflp based genotyping of indian isolates of toxoplasma gondii. | toxoplasma gondii is an apicomplexan parasite capable of infecting a wide variety of warm-blooded animals, including birds and humans and is zoonotically important too. felidae serve its definitive hosts and most infections are inoccous while in various intermediate hosts (e.g. sheep), it is responsible for abortion, still births. humans which are immune compromised are also susceptible to toxoplasmosis. most of the epidemiological studies have revealed it to be belonging to three clonal types w ... | 2017 | 28615877 |
| anti-toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: the first report in the americas. | in brazil, there have been no previous studies of toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. this study evaluated t. gondii infection in patients with beta-hemoglobinopathies. | 2017 | 28615064 |
| identification of compounds that suppress toxoplasma gondii tachyzoites and bradyzoites. | drug treatment for toxoplasmosis is problematic, because current drugs cannot eradicate latent infection with toxoplasma gondii and can cause bone marrow toxicity. because latent infection remains after treatment, relapse of infection is a problem in both infections in immunocompromised patients and in congenitally infected patients. to identify lead compounds for novel drugs against toxoplasma gondii, we screened a chemical compound library for anti-toxoplasma activity, host cell cytotoxicity, ... | 2017 | 28609444 |
| transcriptional responses in the murine spleen after toxoplasma gondii infection: inflammasome and mucus-associated genes. | the spleen plays an important role in coordinating both adaptive and innate immune responses. here, the transcriptional response to t. gondii infection in the murine spleen was characterized concerning inflammasome sensors (two different models: seven days after oral or four weeks after intraperitoneal infection). additionally, tff1(ko) and tff3(ko) mice were investigated because tff genes are often upregulated during inflammation. the expression of the pattern-recognition receptors nlrp3, nlrp1 ... | 2017 | 28604600 |
| the effect of tgf-β on treg cells in adverse pregnancy outcome upon toxoplasma gondii infection. | toxoplasma gondii (t. gondii) is a ubiquitous intracellular protozoan parasite that causes adverse pregnancy outcomes. its infection downregulates the treg cell population and tgf-β level, which may contribute to adverse pregnancy outcomes. tgf-β plays a critical role in treg cell development, but whether tgf-β treatment can affect the number and function of treg cells and hence alleviate adverse pregnancy outcomes caused by t. gondii infection remains elusive. in this study, t. gondii-infected ... | 2017 | 28603517 |
| sag5b and sag5c combined vaccine protects mice against toxoplasma gondii infection. | infections with the protozoan parasite toxoplasma gondii, which are common around the world, can lead to congenital infections in humans. t. gondii surface antigen protein 5b (sag5b) and sag5c are potential stimulators of humoral and cellular immune responses. in this study, a multi-antigenic dna vaccine constructed to express t. gondii sag5b and sag5c proteins simultaneously was used to immunize balb/c mice to evaluate the protective efficacy of the vaccine. igg antibody and gamma interferon (i ... | 2017 | 28602862 |
| parkinson's disease and toxoplasma gondii infection: sero-molecular assess the possible link among patients. | we investigated the possible association between parkinson's disease (pd), the second most common neurodegenerative disorder and toxoplasma gondii infection, the most common neurotropic protozoan parasitic infection, using serological and molecular techniques. one hundred and fifteen patients with confirmed pd and 115 healthy subjects in the same age and sex distribution were enrolled in this study. blood samples were taken from each participant and the sera was screened for anti-toxoplasma anti ... | 2017 | 28602836 |
| the antifungal aureobasidin a and an analogue are active against the protozoan parasite toxoplasma gondii but do not inhibit sphingolipid biosynthesis - corrigendum. | 2017 | 28595661 | |
| myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of toxoplasma gondii. | the obligate intracellular parasite toxoplasma gondii possesses a repertoire of 11 myosins. three class xiv motors participate in motility, invasion and egress, whereas the class xxii myosin f is implicated in organelle positioning and inheritance of the apicoplast. here we provide evidence that tgunc acts as a chaperone dedicated to the folding, assembly and function of all toxoplasma myosins. the conditional ablation of tgunc recapitulates the phenome of the known myosins and uncovers two func ... | 2017 | 28593938 |