Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| rapid fluorescence-based measurement of toxicity in anaerobic digestion. | a rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. by initially using a pure facultative anaerobic strain, enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (pcp). the technique revealed significant metabolic changes in enterococcus faecalis with a pcp spike ranging from 0.05 to 100 mg/l, and ... | 2015 | 25768985 |
| could β-hemolytic, group b enterococcus faecalis be mistaken for streptococcus agalactiae? | a β-hemolytic enterococcus faecalis strain agglutinating lancefield group a, b, c, d, f, and g antisera was observed from a rectovaginal swab, in the context of antenatal screening for streptococcus agalactiae (group b streptococcus [gbs]). this is the first multi-lancefield antisera-agglutinating isolate of this species, and it raised particular concern, as it may mimic gbs, leading to false reporting and useless receipt of intrapartum antibiotics. | 2015 | 25766004 |
| re: enterococcus faecalis subverts and invades the host urothelium in patients with chronic urinary tract infection. | 2015 | 25765392 | |
| bacteriological findings and antimicrobial susceptibility pattern of isolated pathogens from visual threatening ocular infections. | to study the profiles and antimicrobial susceptibility pattern of isolated bacteria from visual threatening ocular infection. | 2015 | 25764616 |
| fluoroquinolone resistance in bacteremic and low risk febrile neutropenic patients with cancer. | the low risk febrile neutropenic patients with multinational association for supportive care in cancer (mascc) score of more than 20 are recommended to be treated with fluoroquinolone-based oral treatment by the national comprehensive cancer network (nccn) guideline. this recommendation relies, at least partially, on the high sensitivity of the blood culture isolates to fluoroquinolone in clinical trials conducted in western countries. whether this also applies in middle or low income countries ... | 2015 | 25763661 |
| antimicrobial action of oleanolic acid on listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. | this study investigated the antimicrobial action of oleanolic acid against listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. to determine the cytotoxicity of oleanolic acid, hep-2 cells were incubated with oleanolic acid at 37°c. mics (minimal inhibition concentrations) for l. monocytogenes, e. faecium, and e. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid a ... | 2015 | 25756202 |
| β-lactams enhance daptomycin activity against vancomycin-resistant enterococcus faecalis and enterococcus faecium in in vitro pharmacokinetic/pharmacodynamic models. | enterococcus faecalis and enterococcus faecium are frequently resistant to vancomycin and β-lactams. in enterococcal infections with reduced glycopeptide susceptibility, combination therapy is often administered. our objective was to conduct pharmacokinetic/pharmacodynamic (pk/pd) models to evaluate β-lactam synergy with daptomycin (dap) against resistant enterococci. one e. faecalis strain (r6981) and two e. faecium strains (r6370 and 8019) were evaluated. dap mics were obtained. all strains we ... | 2015 | 25753639 |
| fluid dynamics and biofilm removal generated by syringe-delivered and 2 ultrasonic-assisted irrigation methods: a novel experimental approach. | thorough understanding of fluid dynamics in root canal irrigation and corresponding antibiofilm capacity will support improved disinfection strategies. this study aimed to develop a standardized, simulated root canal model that allows real-time analysis of fluid/irrigation dynamics and its correlation with biofilm elimination. | 2015 | 25749254 |
| ag-loaded mesoporous bioactive glasses against enterococcus faecalis biofilm in root canal of human teeth. | ag-loaded mesoporous bioactive glass (ag-mbg) powders were synthesized and characterized. the ions release of ag-mbgs in tris-hcl and the ph stability of simulated body fluids after immersing ag-mbgs were tested. root canals were inoculated with enterococcus faecalis for 4 weeks, and the antibacterial activity of mbgs, ag-mbgs and calcium hydroxide against e. faecalis biofilm were evaluated. results showed that ag-mbgs possessed highly ordered mesoporous structure with silver nanoparticles depos ... | 2015 | 25748459 |
| [analysis of pathogenic bacteria and drug resistance in neonatal purulent meningitis]. | to study the clinical characteristics, pathogenic bacteria, and antibiotics resistance of neonatal purulent meningitis in order to provide the guide for early diagnosis and appropriate treatment. | 2015 | 25748405 |
| molecular mechanisms of resistance to human pathogenic bacteria in caenorhabditis elegans by mev-1 mediated oxidative stress. | both mutation and knockdown in mev-1 gene render caenorhabditis elegans susceptibility to enterococcus faecalis infection. however, the mechanisms by which of mev-1 defects pathogen resistance remain unclear. here we show that mev-1rnai causes a dramatic decrease in oxidative stress and antioxidant enzyme expressions, thereby leading to increased e. faecalis accumulation in nematode intestine. mitochondrial superoxide change after infection induced these oxidative responses through daf-16 activi ... | 2015 | 25747713 |
| new antimicrobial contact catalyst killing antibiotic resistant clinical and waterborne pathogens. | microbial growth on medical and technical devices is a big health issue, particularly when microorganisms aggregate to form biofilms. moreover, the occurrence of antibiotic-resistant bacteria in the clinical environment is dramatically growing, making treatment of bacterial infections very challenging. in search of an alternative, we studied a novel antimicrobial surface coating based on micro galvanic elements formed by silver and ruthenium with surface catalytic properties. the antimicrobial c ... | 2015 | 25746238 |
| the effect of indomethacin and betamethasone on the cytokine response of human neonatal mononuclear cells to gram-positive bacteria. | intrauterine infections with gram-positive bacteria pose a serious threat to neonates since they can result in neonatal sepsis, induce a fetal inflammatory response and also cause preterm birth. despite intensive care, prematurity remains a leading cause of neonatal death, and is often accompanied by a number of morbidities. in order to prevent premature birth, tocolytic agents like indomethacin are administered. betamethasone is used to promote lung maturation and prevent respiratory distress s ... | 2015 | 25743243 |
| whole-genome mapping of 5' rna ends in bacteria by tagged sequencing: a comprehensive view in enterococcus faecalis. | enterococcus faecalis is the third cause of nosocomial infections. to obtain the first snapshot of transcriptional organizations in this bacterium, we used a modified rna-seq approach enabling to discriminate primary from processed 5' rna ends. we also validated our approach by confirming known features in escherichia coli. we mapped 559 transcription start sites (tsss) and 352 processing sites (psss) in e. faecalis. a blind motif search retrieved canonical features of siga- and sign-dependent p ... | 2015 | 25737579 |
| the fsr quorum-sensing system and cognate gelatinase orchestrate the expression and processing of proprotein ef_1097 into the mature antimicrobial peptide enterocin o16. | a novel antimicrobial peptide designated enterocin o16 was purified from enterococcus faecalis. mass spectrometry showed a monoisotopic mass of 7,231 da, and n-terminal edman degradation identified a 29-amino-acid sequence corresponding to residues 90 to 119 of the ef_1097 protein. bioinformatic analysis showed that enterocin o16 is composed of the 68 most c-terminal residues of the ef_1097 protein. introduction of an in-frame isogenic deletion in the ef1097 gene abolished the production of ente ... | 2015 | 25733609 |
| ec300: a phage-based, bacteriolysin-like protein with enhanced antibacterial activity against enterococcus faecalis. | bacteriophage lytic enzymes, either endolysins or virion-associated lysins, have been receiving considerable attention as potential antibacterial agents, particularly for the combat of antibiotic-resistant gram-positive pathogens. a conclusion that easily emerges from the careful analysis of a great number of reports on the field is that the activity of phage lytic enzymes is rarely studied in conditions that support robust growth of the target bacteria. here, we report the construction and stud ... | 2015 | 25728445 |
| the sfani restriction-modification system from enterococcus faecalis neb215 is located on a putative mobile genetic element. | a type iis restriction-modification (r-m) system sfani from enterococcus faecalis neb215 has been characterized. the sfanim gene was cloned by the methylase selection method. methyltransferase sfani, a protein of 695 amino acids, consists of two domains responsible for different dna-strand recognition and modification, and a putative dna-binding hth domain located in the n-terminal part of the protein. the sfanir gene, located adjacent to the gene of the cognate modification methyltransferases, ... | 2015 | 25724535 |
| validation of a novel rinse and filtration method for efficient processing of fresh produce samples for microbiological indicator enumeration. | several methods have been described to prepare fresh produce samples for microbiological analysis, each with its own advantages and disadvantages. the aim of this study was to compare the performance of a novel combined rinse and membrane filtration method to two alternative sample preparation methods for the quantification of indicator microorganisms from fresh produce. decontaminated cantaloupe melons and jalapeño peppers were surface inoculated with a cocktail containing 10(6) cfu/ml escheric ... | 2015 | 25719876 |
| prevalence, resistance patterns, and risk factors for antimicrobial resistance in bacteria from retail chicken meat in colombia. | as a step toward implementing the colombian integrated program for antimicrobial resistance surveillance (coipars), this study aimed to establish the baseline antimicrobial resistance patterns of salmonella serovars, escherichia coli, and enterococcus spp. isolates in retail poultry meat from independent stores and from a main chain distributor center. mics of the isolates were determined for antimicrobials used both in humans and animals, using an automated system. salmonella serovars were isol ... | 2015 | 25836401 |
| anaerobic degradation of tannins in acacia nilotica pods by enterococcus faecalis in co-culture with ruminal microbiota. | 2015 | 25833679 | |
| exploration of the mechanisms of biofilm formation by enterococcus faecalis in glucose starvation environments. | enterococcus faecalis is the most common single species present in teeth after failed root canal therapy. this is mainly due to its ability to maintain viability for a long time in filled root canals where nutrients are normally sparse. the aim of this study was to explore the mechanism of e. faecalis survival and biofilm formation in glucose-starved environments. | 2015 | 25990488 |
| collagen degradation and mmp9 activation by enterococcus faecalis contribute to intestinal anastomotic leak. | even under the most expert care, a properly constructed intestinal anastomosis can fail to heal, resulting in leakage of its contents, peritonitis, and sepsis. the cause of anastomotic leak remains unknown, and its incidence has not changed in decades. we demonstrate that the commensal bacterium enterococcus faecalis contributes to the pathogenesis of anastomotic leak through its capacity to degrade collagen and to activate tissue matrix metalloproteinase 9 (mmp9) in host intestinal tissues. we ... | 2015 | 25947163 |
| dissemination of enterococcus faecalis and enterococcus faecium in a ricotta processing plant and evaluation of pathogenic and antibiotic resistance profiles. | in this work, the sources of contamination by enterococcus spp. in a ricotta processing line were evaluated. the isolated strains were tested for virulence genes (gele, cyla,b, m, esp, agg, ace, efaa, vanb), expression of virulence factors (hemolysin and gelatinase), and the resistance to 10 different antibiotics. enterococcus faecium and enterococcus faecalis were subjected to discriminatory identification by intergenic spacer region (its)-polymerase chain reaction and sequencing of the its reg ... | 2015 | 25874819 |
| in vitro evaluation of antimicrobial photodynamic therapy associated with hydroalcoholic extracts of schinopsis brasiliensis engl.: new therapeutic perspectives. | the aim of this study was to evaluate the photodynamic potential of extracts of schinopsis brasiliensis engl. on bacteria involved in several human infections. | 2015 | 25866862 |
| incidence, risk factors, microbiology of venous catheter associated bloodstream infections--a prospective study from a tertiary care hospital. | central venous catheters (cvcs) though indispensable in current medical and intensive care treatment, also puts patients at risk of catheter related infection (cri) resulting in increased morbidity and mortality. we analysed the incidence, risk factors, bacteriological profile and antimicrobial susceptibility pattern of the isolates in central venous catheter associated bloodstream infection (cvc-bsi) in the intensive care unit (icu) patients and studied the formation of biofilm in cvcs. | 2015 | 25865976 |
| semi-synthesis of new antimicrobial esters from the natural oleanolic and maslinic acids. | in this article, we report an effective procedure for the selective isolation of oleanolic acid 1 and maslinic acid 2 (3.4 and 8.5mg/g dw, respectively) from pomace olive (olea europaea l.) using an ultrasonic bath, and the synthesis of a series of new triterpenic acid esters. the compounds were characterized by their spectral data and were evaluated for their antimicrobial activity. among the compounds tested, those having sulfur and chlorine atoms were found to be antibacterial. they showed ac ... | 2015 | 25863603 |
| evaluation of antimicrobial effectiveness and dentine mechanical properties after use of chemical and natural auxiliary irrigants. | to evaluate the effect of gse, naocl, chx and qmix as an antimicrobial agents against enterococcus faecalis and their influence on flexural and ultimate tensile strength of root canal dentine. | 2015 | 25863158 |
| erratum for la rosa et al., in vivo assessment of growth and virulence gene expression during commensal and pathogenic lifestyles of luxabcde-tagged enterococcus faecalis strains in murine gastrointestinal and intravenous infection models. | 2015 | 25862783 | |
| the conserved modular elements of the acyl carrier proteins of lipid synthesis are only partially interchangeable. | prior work showed that expression of acyl carrier proteins (acps) of a diverse set of bacteria replaced the function of escherichia coli acp in lipid biosynthesis. however, the acpas of lactococcus lactis and enterococcus faecalis were inactive. both failed to support growth of an e. coli acpp mutant strain. this defect seemed likely because of the helix ii sequences of the two acpas, which differed markedly from those of the proteins that supported growth. to test this premise, chimeric acps we ... | 2015 | 25861991 |
| emergence of multidrug resistant enterococci at a tertiary care centre. | enterococci have assumed great clinical importance because of their increasing resistance to various antimicrobial agents. thus, knowledge about the antibiogram of these multidrug resistant isolates is of utmost importance in formulating an effective antibiotic policy to treat these infections and reducing the morbidity and mortality. aim of this study was to assess the antimicrobial resistance pattern of enterococci and determine the prevalence of multidrug resistance among them. | 2015 | 25859076 |
| urinary tract infections: epidemiology, mechanisms of infection and treatment options. | urinary tract infections (utis) are a severe public health problem and are caused by a range of pathogens, but most commonly by escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterococcus faecalis and staphylococcus saprophyticus. high recurrence rates and increasing antimicrobial resistance among uropathogens threaten to greatly increase the economic burden of these infections. in this review, we discuss how basic science studies are elucidating the molecular details of the crossta ... | 2015 | 25853778 |
| effect of apically separated rotary instruments on bacterial leakage of obturated root canals using resilon. | the purpose of this study was to determine the effect of a separated rotary instrument on the time required for bacterial penetration of obturated root canals using resilon. eighty-four extracted human maxillary premolars with single canals were used in this study. group 1 consisted of teeth obturated with gutta-percha and ah-26 sealer (n=15); group 2 consisted of teeth obturated similarly to group 1 but without a separated file; group 3 consisted of teeth obturated with resilon and epiphany sea ... | 2015 | 25928974 |
| genotypic variations of virulent genes in enterococcus faecium and enterococcus faecalis isolated from three hospitals in malaysia. | the genus enterococcus is of increasing significance as a cause of nosocomial infections, and this trend is exacerbated by the development of antibiotic resistance. | 2015 | 25923096 |
| multiple roles for enterococcus faecalis glycosyltransferases in biofilm-associated antibiotic resistance, cell envelope integrity, and conjugative transfer. | the emergence of multidrug-resistant bacteria and the limited availability of new antibiotics are of increasing clinical concern. a compounding factor is the ability of microorganisms to form biofilms (communities of cells encased in a protective extracellular matrix) that are intrinsically resistant to antibiotics. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and also has the propensity to acquire resistance determinants via horizontal gene transfer. there is i ... | 2015 | 25918141 |
| effects of photodynamic therapy on enterococcus faecalis biofilms. | microbial biofilms are involved in almost all infectious pathologies of the oral cavity. this has led to the search for novel therapies specifically aimed at biofilm elimination. in this study, we used atomic force microscopy (afm) to visualize injuries and to determine surface roughness, as well as confocal laser scanning microscopy (clsm) to enumerate live and dead bacterial cells, to determine the effects of photodynamic therapy (pdt) on enterococcus faecalis biofilms. the afm images showed t ... | 2015 | 25917515 |
| enterococcus faecalis gelatinase mediates intestinal permeability via protease-activated receptor 2. | microbial protease-mediated disruption of the intestinal epithelium is a potential mechanism whereby a dysbiotic enteric microbiota can lead to disease. this mechanism was investigated using the colitogenic, protease-secreting enteric microbe enterococcus faecalis. caco-2 and t-84 epithelial cell monolayers and the mouse colonic epithelium were exposed to concentrated conditioned media (ccm) from e. faecalis v583 and e. faecalis lacking the gelatinase gene (gele). the flux of fluorescein isothio ... | 2015 | 25916983 |
| proteomic investigation of the response of enterococcus faecalis v583 when cultivated in urine. | enterococcus faecalis is a robust bacterium, which is able to survive in and adapt to hostile environments such as the urinary tract and bladder. in this label-free quantitative proteomic study based on maxquant lfq algorithms, we identified 127 proteins present in the secretome of the clinical vancomycin-resistant isolate e. faecalis v583 and we compared proteins secreted in the initial phase of cultivation in urine with the secretome during cultivation in standard laboratory medium, 2xyt. of t ... | 2015 | 25915650 |
| tyrosine decarboxylase activity of enterococci grown in media with different nutritional potential: tyramine and 2-phenylethylamine accumulation and tyrdc gene expression. | the ability to accumulate tyramine and 2-phenylethylamine by two strains of enterococcus faecalis and two strains enterococcus faecium was evaluated in two cultural media added or not with tyrosine. all the enterococcal strains possessed a tyrosine decarboxylase (tyrdc) which determined tyramine accumulation in all the conditions tested, independently on the addition of high concentration of free tyrosine. enterococci differed in rate and level of biogenic amines accumulation. e. faecalis ef37 a ... | 2015 | 25914676 |
| preparation, characterization and antibacterial properties of zno/kaoline nanocomposites. | this paper describes laboratory preparation, characterization and antibacterial activity testing of zno/kaoline composites. zno/kaoline composites with 50 wt.% of zno were laboratory prepared, dried at 105 °c and calcined at 500 °c. xrpd analysis revealed that thermal treatment caused the phase transformation of zn containing precursor into zno. scanning and transmission electron microscopy techniques were used for characterization of morphology of the prepared samples. a standard microdilution ... | 2015 | 25909474 |
| interplay between copper and zinc homeostasis through the transcriptional regulator zur in enterococcus faecalis. | by integrating the microarray expression data and a global e. faecalis transcriptional network we identified a sub-network activated by zinc and copper. our analyses indicated that the transcriptional response of the bacterium to copper and zinc exposure involved the activation of two modules, module i that contains genes implicated in zinc homeostasis, including the zur transcriptional repressor, and module ii containing a set of genes associated with general stress response and basal metabolis ... | 2015 | 25906431 |
| ph-induced change in cell susceptibility to butanol in a high butanol-tolerant bacterium, enterococcus faecalis strain cm4a. | though butanol is considered as a potential biofuel, its toxicity toward microorganisms is the main bottleneck for the biological butanol production. recently, butanol-tolerant bacteria have been proposed as alternative butanol production hosts overcoming the end product inhibition. one remaining key issue to be addressed is how physicochemical properties such as ph and temperature affect microbial butanol tolerance during cultivation and fermentation. | 2015 | 25904984 |
| genotypic and phenotypic diversity in enterococcus faecalis: is agar invasion a pathogenicity score? | the main objective of the present study is to analyze different genotypic and phenotypic traits related to virulence in enterococcus faecalis, as well as evaluated the agar invasion phenotype in a collection of isolates with different clinical origins. | 2015 | 25904518 |
| competition between vanu(g) repressor and vanr(g) activator leads to rheostatic control of vang vancomycin resistance operon expression. | enterococcus faecalis bm4518 is resistant to vancomycin by synthesis of peptidoglycan precursors ending in d-alanyl-d-serine. in the chromosomal vang locus, transcription of the resistance genes from the pyg resistance promoter is inducible and, upstream from these genes, there is an unusual three-component regulatory system encoded by the vanurs(g) operon from the p(ug) regulatory promoter. in contrast to the other van operons in enterococci, the vang operon possesses the additional vanu(g) gen ... | 2015 | 25898178 |
| a variable dna recognition site organization establishes the liar-mediated cell envelope stress response of enterococci to daptomycin. | liar is a 'master regulator' of the cell envelope stress response in enterococci and many other gram-positive organisms. mutations to liar can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. liar is phosphorylated in response to membrane stress to regulate downstream target operons. using dna footprinting of the regions upstream of the liaxyz and liafsr operons we show that liar binds an extended stretch of dna that extends beyond the propos ... | 2015 | 25897118 |
| urinary tract infections and antimicrobial sensitivity among diabetic patients at khartoum, sudan. | patients with diabetes mellitus (dm) are more susceptible to urinary tract infection (uti) than non-diabetics. due to the emergence of multidrug resistant (mdr) uropathogenic strains, the choice of antimicrobial agent is restricted. this study investigated the epidemiology of uti, antimicrobial susceptibility, and resistance patterns of bacterial isolates from adult diabetic patients. | 2015 | 25896611 |
| total and specific bacterial levels in the apical root canal system of teeth with post-treatment apical periodontitis. | most studies of the microbiota in root canal-treated teeth focused only on the main canal, not distinguishing regions nor incorporating the intricate anatomy in the analysis. moreover, most of them provided only prevalence data. this study was designed to evaluate the total bacterial counts and the presence, levels, and relative abundance of candidate endodontic pathogens exclusively in the apical root canal system associated with post-treatment apical periodontitis. | 2015 | 25892512 |
| minimizing concentration of sodium hypochlorite in root canal irrigation by combination of ultrasonic irrigation with photodynamic treatment. | concentration of sodium hypochlorite (naocl) is positively correlated with its effectiveness in root canal disinfection but negatively correlated with its biocompatibility. the objective of this in vitro study was to compare the bactericidal effects among ultrasonic irrigation with different concentration of naocl alone or together with photodynamic treatment (pdt) against enterococcus faecalis (e. faecalis) in infected root canals. one hundred and twenty bovine root canals contaminated with e. ... | 2015 | 25892274 |
| micropan: an r-package for microbial pan-genomics. | a pan-genome is defined as the set of all unique gene families found in one or more strains of a prokaryotic species. due to the extensive within-species diversity in the microbial world, the pan-genome is often many times larger than a single genome. studies of pan-genomes have become popular due to the easy access to whole-genome sequence data for prokaryotes. a pan-genome study reveals species diversity and gene families that may be of special interest, e.g because of their role in bacterial ... | 2015 | 25888166 |
| evaluation of residual antibacterial effect of human radicular dentin treated with triple and double antibiotic pastes. | the aim of this study was to investigate the residual antibacterial effect of human radicular dentin treated with various concentrations of triple antibiotic paste and double antibiotic paste (dap). | 2015 | 25887806 |
| comprehensive molecular, genomic and phenotypic analysis of a major clone of enterococcus faecalis mlst st40. | enterococcus faecalis is a multifaceted microorganism known to act as a beneficial intestinal commensal bacterium. it is also a dreaded nosocomial pathogen causing life-threatening infections in hospitalised patients. isolates of a distinct mlst type st40 represent the most frequent strain type of this species, distributed worldwide and originating from various sources (animal, human, environmental) and different conditions (colonisation/infection). since enterococci are known to be highly recom ... | 2015 | 25887115 |
| investigating the mobilome in clinically important lineages of enterococcus faecium and enterococcus faecalis. | the success of enterococcus faecium and e. faecalis evolving as multi-resistant nosocomial pathogens is associated with their ability to acquire and share adaptive traits, including antimicrobial resistance genes encoded by mobile genetic elements (mges). here, we investigate this mobilome in successful hospital associated genetic lineages, e. faecium sequence type (st)17 (n=10) and st78 (n=10), e. faecalis st6 (n=10) and st40 (n=10) by dna microarray analyses. | 2015 | 25885771 |
| genetic relatedness and risk factor analysis of ampicillin-resistant and high-level gentamicin-resistant enterococci causing bloodstream infections in tanzanian children. | while enterococci resistant to multiple antimicrobials are spreading in hospitals worldwide, causing urinary tract, wound and bloodstream infections, there is little published data on these infections from africa. | 2015 | 25884316 |
| review and phylogenetic analysis of qac genes that reduce susceptibility to quaternary ammonium compounds in staphylococcus species. | the qac genes of staphylococcus species encode multidrug efflux pumps: membrane proteins that export toxic molecules and thus increase tolerance to a variety of compounds such as disinfecting agents, including quaternary ammonium compounds (for which they are named), intercalating dyes and some antibiotics. in stapylococcus species, six different plasmid-encoded qac efflux pumps have been described, and they belong to two major protein families. qaca and qacb are members of the major facilitator ... | 2015 | 25883793 |
| iatrogenic aortic insufficiency following mitral valve replacement: case report and review of the literature. | we report a 28-year-old white female who suffered significant aortic insufficiency (ai) following mitral valve (mv) replacement for endocarditis. the patient had history of rheumatoid arthritis and presented to our emergency department with a 3-month history of dyspnea, orthopnea, fevers and weight loss, worsening over 2 weeks, for which she took intermittent acetaminophen. on admission, vital signs revealed blood pressure of 99/70 mm hg, heart rate of 120 beats/minute, and temperature of 98.8 ° ... | 2015 | 25883714 |
| antibacterial and cytotoxic activity from the extract and fractions of a marine derived bacterium from the streptomyces genus. | marine natural products are a rich source of potent, selective, and structurally novel compounds. marine bacteria are considered the most promising source of biologically active compounds which can be applied to treat a wide range of diseases. | 2015 | 25880141 |
| antibiotic selective pressure and development of bacterial resistance detected in bacteriuria following kidney transplantation. | bacteriuria (symptomatic and asymptomatic) is the most common infectious complication after kidney transplantation. this study aimed to determine its prevalence among kidney transplant recipients hospitalized after transplantation, respective risk factors, and frequency of isolates and antibacterial susceptibility. | 2015 | 26036537 |
| surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic escherichia coli biofilms against pathogen colonization. | prevention of pathogenic colonization on medical devices over a long period of time remains a great challenge, especially in a high-nutrient environment that accelerates production of biomass leading to biofouling of the device. since biofouling and the subsequent pathogen colonization is eventually inevitable, a new strategy using non-pathogenic bacteria as living guards against pathogenic colonization on medical devices has attracted increasing interest. crucial to the success of this strategy ... | 2015 | 26029359 |
| evaluation of cold plasma treatment and safety in disinfecting 3-week root canal enterococcus faecalis biofilm in vitro. | although endodontic infection is caused by multi-bacteria species, enterococcus faecalis is usually isolated in chronic apical periodontitis. the aim of this study was to evaluate the effectiveness and mechanical safety of cold plasma therapy in disinfecting 3-week e. faecalis biofilms. | 2015 | 26027875 |
| susceptibility patterns of uropathogens identified in hospitalised children with community-acquired urinary tract infections in thrace, greece. | urinary tract infections (utis) are common infections associated with considerable morbidity and mortality, particularly in paediatric patients. the alarmingly increasing antimicrobial resistance of contemporary uropathogens in children necessitates the re-evaluation of antibiotic treatment. we evaluated uropathogens isolated from children hospitalised due to a community-acquired uti over a 5.5-year period in a university hospital of northern greece and their antibiotic susceptibility patterns. ... | 2015 | 27873675 |
| evaluation of the effect of enterococcus faecalis biofilm on the 2% chlorhexidine substantivity: an in vitro study. | the aim of this study was to correlate the bacterial viability and the presence of 2% chlorhexidine (chx) solution on dentin by means of confocal laser scanning microscopy and high-performance liquid chromatography for 48 hours, 7 days, and 30 days. | 2015 | 26025346 |
| statins and antimicrobial effects: simvastatin as a potential drug against staphylococcus aureus biofilm. | statins are important lipid-lowering agents with other pleiotropic effects. several studies have explored a possible protective effect of statins to reduce the morbidity and mortality of many infectious diseases. staphylococcus aureus is one of the main pathogens implicated in nosocomial infections; its ability to form biofilms makes treatment difficult. the present study observed the mic of atorvastatin, pravastatin and simvastatin against s. aureus, pseudomonas aeruginosa, escherichia coli and ... | 2015 | 26020797 |
| analysis of vancomycin susceptibility testing results for presumptive categorization of telavancin. | scattergrams between vancomycin and telavancin demonstrated susceptibility agreement rates of 99.96, 99.65, and 100.00% for staphylococcus aureus, enterococcus faecalis, and streptococci, respectively. a single very major error was obtained against e. faecalis, while vancomycin-intermediate s. aureus (visa) and vancomycin-resistant and teicoplanin-susceptible (vanb phenotype) e. faecalis were responsible for major and minor errors. these results support the use of vancomycin to infer telavancin ... | 2015 | 26019194 |
| a novel termini analysis theory using hts data alone for the identification of enterococcus phage ef4-like genome termini. | enterococcus faecalis and enterococcus faecium are typical enterococcal bacterial pathogens. antibiotic resistance means that the identification of novel e. faecalis and e. faecium phages against antibiotic-resistant enterococcus have an important impact on public health. in this study, the e. faecalis phage ime-ef4, e. faecium phage ime-efm1, and both their hosts were antibiotic resistant. to characterize the genome termini of these two phages, a termini analysis theory was developed to provide ... | 2015 | 26016565 |
| the enterococcus faecalis ebpa pilus protein: attenuation of expression, biofilm formation, and adherence to fibrinogen start with the rare initiation codon att. | the endocarditis and biofilm-associated pili (ebp) are important in enterococcus faecalis pathogenesis, and the pilus tip, ebpa, has been shown to play a major role in pilus biogenesis, biofilm formation, and experimental infections. based on in silico analyses, we previously predicted that att is the ebpa translational start codon, not the atg codon, 120 bp downstream of att, which is annotated as the translational start. att is rarely used to initiate protein synthesis, leading to our hypothes ... | 2015 | 26015496 |
| correlation between enterococcus faecalis and persistent intraradicular infection compared with primary intraradicular infection: a systematic review. | the relationship between enterococcus faecalis and pulpal or periradicular diseases has been studied for many years; however, whether e. faecalis is correlated with persistent intraradicular infections (teeth after failed endodontic treatments) compared with primary intraradicular infections remains controversial. the objective of this systematic review was to compare the prevalence of e. faecalis in primary and persistent intraradicular infections. | 2015 | 26015157 |
| uses and limitations of green fluorescent protein as a viability marker in enterococcus faecalis: an observational investigation. | enterococci are capable of producing biofilms that are notoriously difficult to treat and remove, for instance in root canal infections. the tenacious nature of these organisms makes screening of known and novel antimicrobial compounds necessary. while traditionally growth and fluorescence-based screening methods have proven useful, these methods have their limitations when applied to enterococci (e.g. time consuming, no kinetic data, diffusion properties of the fluorescent dyes). the aim of thi ... | 2015 | 26015063 |
| identification of enterococcus faecalis and pseudomonas aeruginosa on and in implants in individuals with peri-implant disease: a cross-sectional study. | to verify whether the parts of dental implants can be contaminated by opportunistic pathogens. | 2015 | 26009909 |
| synthesis, structural characterization and biological activity of novel cyclohexane-1,3-dione ligands and their metal complexes. | some new zn(ii) and cu(ii) complexes [cu(l1)(oac)2]∙h2o, [cu(l1)(no3)h2o]∙no3∙3.5h2o, [zn(l1)(no3)2]∙4.5h2o, [zn(l1)(oac)2(h2o)2]∙3h2o, [cu2(l2)(oac)4]∙2h2o∙2dmf, [cu(l2)2]∙2no3∙1.5dmf∙h2o, [zn(l2)2(no3)2]∙dmf and [zn2(l2)(oac)4(h2o)4]∙5h2o; l1 = 2-[2-(2-methoxyphenyl)hydrazono]cyclohexane-1,3-dione and l2 = 2-[2-(3-nitrophenyl)hydrazono]cyclohexane-1,3-dione were synthesized and characterized by ir, 1h-nmr,13c-nmr and ultraviolet (uv-vis.) spectroscopy, elemental analysis, magnetic susceptibili ... | 2015 | 26007185 |
| the mazef toxin-antitoxin system as an attractive target in clinical isolates of enterococcus faecium and enterococcus faecalis. | the toxin-antitoxin (ta) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. in this study, the prevalence of ta systems in independently isolated clinical isolates of enterococcus faecium and enterococcus faecalis was determined, the dominant ta system was identified, different virulence genes in e. faecium and e. faecalis were surveyed, the level of expression of the virulence and ta genes in normal and stress conditions was determined, and f ... | 2015 | 26005332 |
| overexpression of enterococcus faecalis elr operon protects from phagocytosis. | mechanisms underlying the transition from commensalism to virulence in enterococcus faecalis are not fully understood. we previously identified the enterococcal leucine-rich protein a (elra) as a virulence factor of e. faecalis. the elra gene is part of an operon that comprises four other orfs encoding putative surface proteins of unknown function. | 2015 | 26003173 |
| probiotic features of lactobacillus strains isolated from ragusano and pecorino siciliano cheeses. | in the present study 177 lactobacillus spp. strains, isolated from ragusano and pecorino siciliano cheeses, were in vitro screened for probiotic traits, and their characteristics were compared to those of lactobacillus rhamnosus gg, commercial strain. based on acidic and bile salt resistance, thirteen lactobacillus strains were selected. the multiplex-pcr application revealed that nine strains belonged to l. rhamnosus species and four to lactobacillus paracasei species. all selected strains were ... | 2015 | 25998823 |
| association between triclosan-coated sutures for abdominal wall closure and incisional surgical site infection after open surgery in patients presenting with fecal peritonitis: a randomized clinical trial. | a factor that may influence the incidence of surgical site infections (ssis) is the suture used for closure of the abdominal wall because bacteria may adhere to the suture material. sutures can be coated with antibacterial substances that may reduce the bacterial load in the incision. | 2015 | 26171624 |
| histopathological changes induced in an animal model by potentially pathogenic enterococcus faecalis strains recovered from ready-to-eat food outlets in osun state, nigeria. | enterococci have been implicated as an emerging important cause of several diseases and multiple antibiotic resistance. however, there is little information about the prevalence of pathogenic and/or antibiotic-resistant enterococcus faecalis in ready-to-eat foods in nigeria. here we report the pathogenic potential of three selected antibiotic-resistant e. faecalis strains isolated from food canteens and food outlets with different virulence determinant genes, including efc 12 (with gel (+), esp ... | 2015 | 26170700 |
| in vitro effectiveness of brazilian brown propolis against enterococcus faecalis. | the aim of this study was to evaluate the in vitro antimicrobial activity of brazilian brown propolis as an intracanal medication against enterococcus faecalis. thirty dentin discs prepared from intact freshly extracted bovine maxillary central incisors were infected with e. faecalis for 21 days. the specimens were distributed into six groups according to the medicament used as follows: g1- calcium hydroxide paste; g2- carbowax 400 (control group); g3- 20% brown propolis paste; g4- 40% brown pro ... | 2015 | 25992787 |
| a comparison of monomicrobial versus polymicrobial enterococcus faecalis bacteriuria in a french university hospital. | enterococci are of considerable relevance in the hospital setting. their most common location is the urinary tract, where they may be responsible for both colonization and infections. they are often associated with the presence of other microorganisms. the aim was to compare monomicrobial and polymicrobial enterococcus faecalis bacteriuria. a retrospective study was performed on the demographic, clinical, and laboratory data of 299 patients who had presented with e. faecalis bacteriuria in 2012 ... | 2015 | 25987245 |
| infective endocarditis presenting as acute renal failure and unusual complications. | infective endocarditis can be a diagnostic challenge. few cases have so far reported infective endocarditis presenting as renal failure. pseudoaneurysms of the mitral-aortic intervalvular fibrosa and splenic abscess are rare complications of infective endocarditis. we herein report a case of an 80-year-old man admitted due to anorexia, malaise, edema of the legs and renal failure. a progressive degradation of the patient's renal function was documented and hemodialysis was started. blood culture ... | 2015 | 25986267 |
| antimicrobial residual effects of irrigation regimens with maleic acid in infected root canals. | the success of endodontic treatment depends largely on the control of microorganisms present in infected root canals. the aim of this study was to determine the residual antimicrobial activity of several final irrigation protocols with 7% maleic acid (ma) alone and combined with chlorhexidine (chx), cetrimide (ctr) or both, in root canals infected with enterococcus faecalis. biofilms of e. faecalis were grown in uniradicular roots for 4 weeks. a total of 72 specimens were divided into 5 experime ... | 2015 | 25984506 |
| the effect of benzalkonium chloride additions to ah plus sealer. antimicrobial, physical and chemical properties. | the aim of this study was to determine the antimicrobial and antibiofilm activities and physicochemical properties of ah plus sealer mixed with different concentrations of benzalkonium chloride (bc). | 2015 | 25979825 |
| the intraperitoneal transcriptome of the opportunistic pathogen enterococcus faecalis in mice. | enterococcus faecalis is a gram-positive lactic acid intestinal opportunistic bacterium with virulence potential. for a better understanding of the adapation of this bacterium to the host conditions, we performed a transcriptome analysis of bacteria isolated from an infection site (mouse peritonitis) by rna-sequencing. we identified a total of 211 genes with significantly higher transcript levels and 157 repressed genes. our in vivo gene expression database reflects well the infection process si ... | 2015 | 25978463 |
| a novel gene, optra, that confers transferable resistance to oxazolidinones and phenicols and its presence in enterococcus faecalis and enterococcus faecium of human and animal origin. | the oxazolidinone-resistant enterococcus faecalis e349 from a human patient tested negative for the cfr gene and 23s rrna mutations. here we report the identification of a novel oxazolidinone resistance gene, optra, and a first investigation of the extent to which this gene was present in e. faecalis and enterococcus faecium from humans and food-producing animals. | 2015 | 25977397 |
| molecular characterization of resistance, virulence and clonality in vancomycin-resistant enterococcus faecium and enterococcus faecalis: a hospital-based study in beijing, china. | the incidence of vancomycin-resistant enterococcus (vre) in china is increasing, the molecular epidemiology of vre in china is only partly known. this study was conducted to assess the molecular characterization of resistance, virulence and clonality of 69 vancomycin-resistant enterococcus faecium (vrefm) and seven vancomycin-resistant enterococcus faecalis (vrefs) isolates obtained from a chinese hospital between july 2011 and july 2013. the glycopeptide resistance genes (vana and vanb) were sc ... | 2015 | 25976380 |
| which is the most effective disinfection method in primary root canals: conventional or newly developed ones? | the aim of the study was to evaluate the antimicrobial activity of the-potassium-titanyl-phosphate--the ktp laser and ozone in of primary root canals. | 2015 | 25966729 |
| is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583. | enterococcus faecalis is one of the most controversial species of lactic acid bacteria. some strains are used as probiotics, while others are associated with severe and life-threatening nosocomial infections. their pathogenicity depends on the acquisition of multidrug resistance and virulence factors. gelatinase, which is required in the first steps of biofilm formation, is an important virulence determinant involved in e. faecalis pathogenesis, including endocarditis and peritonitis. the gene t ... | 2015 | 25966618 |
| improved antibacterial phototoxicity of a neutral porphyrin in natural deep eutectic solvents. | neutral porphyrins for antibacterial photodynamic therapy (apdt) have received little attention due to their tendency to aggregate in aqueous media and reports of low phototoxic effect. these compounds may be less toxic to cells than positively and negatively charged photosensitisers. the preparation of highly bacterial phototoxic formulations of neutral porphyrins remains an open field of research with great potential if achievable. the purpose of this study was to develop novel hydrophilic for ... | 2015 | 25966307 |
| polyamine derivatives of betulinic acid and β-sitosterol: a comparative investigation. | β-sitosterol and betulinic acid were used in designing their conjugates with selected polyamines bearing either an amide bond, or an ester and an amide bond simultaneously in the target molecule. the synthesized compounds were subjected to basic cytotoxic and antimicrobial tests. the synthetic protocol is described separately for each of the three series of the target amides, because each series of compounds required a different synthetic approach. the cytotoxicity was tested on cells derived fr ... | 2015 | 25963549 |
| synthesis and evaluation of cationic norbornanes as peptidomimetic antibacterial agents. | a series of structurally amphiphilic biscationic norbornanes have been synthesised as rigidified, low molecular weight peptidomimetics of cationic antimicrobial peptides. a variety of charged hydrophilic functionalities were attached to the norbornane scaffold including aminium, guanidinium, imidazolium and pyridinium moieties. additionally, a range of hydrophobic groups of differing sizes were incorporated through an acetal linkage. the compounds were evaluated for antibacterial activity agains ... | 2015 | 25958967 |
| an antibacterial ortho-quinone diterpenoid and its derivatives from caryopteris mongolica. | to identify antibacterial components in traditional mongolian medicinal plant caryopteris mongolica, an ortho-quinone abietane caryopteron a (1) and three its derivatives caryopteron b-d (2-4) were isolated from the roots of the plant together with three known abietanes demethylcryptojaponol (5), 6α-hydroxydemethyl cryptojaponol (6), and 14-deoxycoleon u (7). the chemical structures of these abietane derivatives were elucidated on the basis of spectroscopic data. compounds 1-4 had c-13 methylcyc ... | 2015 | 25958242 |
| antibacterial properties associated with chitosan nanoparticle treatment on root dentin and 2 types of endodontic sealers. | the aim of this study was to evaluate the efficacy of carboxymethyl chitosan (cmcs) and chitosan nanoparticles (cnps) to inactivate bacteria and prevent biofilm formation at sealer-dentin interfaces. | 2015 | 25958178 |
| the effects of sodium hypochlorite and chlorhexidine irrigants on the antibacterial activities of alkaline media against enterococcus faecalis. | sodium hypochlorite (naocl), chlorhexidine (chx) and calcium hydroxide are common intracanal medicaments. the present study aimed to evaluate the effects of naocl and chx on the antibacterial activities of alkaline media against enterococcus faecalis. | 2015 | 25956995 |
| small hospitals matter: insights from the emergence and spread of vancomycin-resistant enterococci in 2 public hospitals in inner brazil. | although vancomycin-resistant enterococci (vre) are reported in brazil since 1996, data on their impact over settings of different complexity are scarce. we performed a study aimed at identifying determinants of vre emergence and spread in a public hospital consortium (comprising 2 hospitals, with 318 and 57 beds) in inner brazil. molecular typing and case-control studies (addressing predictors of acquisition or clonality) were performed. among 122 authocthonous isolates, 106 were enterococcus f ... | 2015 | 25956931 |
| daily variations in pathogenic bacterial populations in a monsoon influenced tropical environment. | changing climatic conditions have influenced the monsoon pattern in recent years. variations in bacterial population in one such tropical environment were observed everyday over two years and point out intra and inter annual changes driven by the intensity of rainfall. vibrio spp. were abundant during the monsoon and so were faecal coliforms. vibrio alginolyticus were negatively influenced by nitrate, whereas, silicate and rainfall positively influenced vibrio parahaemolyticus numbers. it is als ... | 2015 | 25956443 |
| the combination of ampicillin plus ceftaroline is synergistic against enterococcus faecalis. | 2015 | 25953804 | |
| effect of nacl treatments on tyramine biosynthesis of enterococcus faecalis. | the effect of nacl stress (0 to 8%, wt/vol) on the growth and tyramine production in two enterococcus faecalis strains was examined during culture time. the growth of e. faecalis was inhibited by the increase in nacl concentration, but tyramine production was unaffected. tyramine accumulated rapidly during the logarithmic phase of the strains, and the final tyramine levels were approximately 800 μg/ml. relative gene expression of four genes in the tyrosine decarboxylase locus, namely, tyrrs, tyr ... | 2015 | 25951388 |
| cytotoxicity, interaction with dentine and efficacy on multispecies biofilms of a modified salt solution intended for endodontic disinfection in a new in vitro biofilm model. | to investigate the cytotoxicity of a modified salt solution (mss) and evaluate the antimicrobial properties of mss on in vitro biofilm models. | 2015 | 24702260 |
| effectiveness of ktp laser versus 980 nm diode laser to kill enterococcus faecalis in biofilms developed in experimentally infected root canals. | this study aimed to evaluate the antibacterial action of ktp (potassium-titanyl-phosphate) laser irradiations (compared with 980 nm diode laser), associated with conventional endodontic procedures, on enterococcus faecalis biofilms. fifty-six dental roots with single canals were prepared with ni-ti rotary instruments, autoclaved, inoculated with an e. faecalis suspension and incubated for 72 h. they were randomly allocated to control and treatment groups. laser parameters were as follows: power ... | 2015 | 24588799 |
| definitive structural assessment of enterococcal diheteroglycan. | enterococcus faecalis is one of most important nosocomial and often multi-antibiotic resistant pathogens responsible for infections that are difficult to treat. previously, a cell-wall polysaccharide termed diheteroglycan (dhg) was isolated and characterized as a promising vaccine candidate. however, the configuration of its lactic acid (la) residue attached to the galactofuranoside unit was not assessed, although it influences conformation of dhg chain in terms of biological recognition and imm ... | 2015 | 25421964 |
| influence of working length and apical preparation size on apical bacterial extrusion during reciprocating instrumentation. | to evaluate the influence of reciprocating single-file instrumentation with different working lengths (wl) and apical preparation sizes on apical bacterial extrusion. | 2015 | 25087922 |
| mckittrick-wheelock syndrome complicated by enterococcus faecalis endocarditis: a unique combination. | 2015 | 25112151 | |
| the surface rhamnopolysaccharide epa of enterococcus faecalis is a key determinant of intestinal colonization. | enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients. the pathogenesis of e. faecalis infection relies in part on its capacity to colonize the gut. following disruption of intestinal homeostasis, e. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream. to identify and characterize e. faecalis genes that are key to intestinal colonization, our strategy consisted in scr ... | 2015 | 25035517 |
| antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or er:yag laser in vitro. | to investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of enterococcus faecalis in vitro. | 2015 | 25080240 |