Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antimicrobial residual effects of irrigation regimens with maleic acid in infected root canals. | the success of endodontic treatment depends largely on the control of microorganisms present in infected root canals. the aim of this study was to determine the residual antimicrobial activity of several final irrigation protocols with 7% maleic acid (ma) alone and combined with chlorhexidine (chx), cetrimide (ctr) or both, in root canals infected with enterococcus faecalis. biofilms of e. faecalis were grown in uniradicular roots for 4 weeks. a total of 72 specimens were divided into 5 experime ... | 2015 | 25984506 |
| the effect of benzalkonium chloride additions to ah plus sealer. antimicrobial, physical and chemical properties. | the aim of this study was to determine the antimicrobial and antibiofilm activities and physicochemical properties of ah plus sealer mixed with different concentrations of benzalkonium chloride (bc). | 2015 | 25979825 |
| the intraperitoneal transcriptome of the opportunistic pathogen enterococcus faecalis in mice. | enterococcus faecalis is a gram-positive lactic acid intestinal opportunistic bacterium with virulence potential. for a better understanding of the adapation of this bacterium to the host conditions, we performed a transcriptome analysis of bacteria isolated from an infection site (mouse peritonitis) by rna-sequencing. we identified a total of 211 genes with significantly higher transcript levels and 157 repressed genes. our in vivo gene expression database reflects well the infection process si ... | 2015 | 25978463 |
| a novel gene, optra, that confers transferable resistance to oxazolidinones and phenicols and its presence in enterococcus faecalis and enterococcus faecium of human and animal origin. | the oxazolidinone-resistant enterococcus faecalis e349 from a human patient tested negative for the cfr gene and 23s rrna mutations. here we report the identification of a novel oxazolidinone resistance gene, optra, and a first investigation of the extent to which this gene was present in e. faecalis and enterococcus faecium from humans and food-producing animals. | 2015 | 25977397 |
| molecular characterization of resistance, virulence and clonality in vancomycin-resistant enterococcus faecium and enterococcus faecalis: a hospital-based study in beijing, china. | the incidence of vancomycin-resistant enterococcus (vre) in china is increasing, the molecular epidemiology of vre in china is only partly known. this study was conducted to assess the molecular characterization of resistance, virulence and clonality of 69 vancomycin-resistant enterococcus faecium (vrefm) and seven vancomycin-resistant enterococcus faecalis (vrefs) isolates obtained from a chinese hospital between july 2011 and july 2013. the glycopeptide resistance genes (vana and vanb) were sc ... | 2015 | 25976380 |
| which is the most effective disinfection method in primary root canals: conventional or newly developed ones? | the aim of the study was to evaluate the antimicrobial activity of the-potassium-titanyl-phosphate--the ktp laser and ozone in of primary root canals. | 2015 | 25966729 |
| is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583. | enterococcus faecalis is one of the most controversial species of lactic acid bacteria. some strains are used as probiotics, while others are associated with severe and life-threatening nosocomial infections. their pathogenicity depends on the acquisition of multidrug resistance and virulence factors. gelatinase, which is required in the first steps of biofilm formation, is an important virulence determinant involved in e. faecalis pathogenesis, including endocarditis and peritonitis. the gene t ... | 2015 | 25966618 |
| improved antibacterial phototoxicity of a neutral porphyrin in natural deep eutectic solvents. | neutral porphyrins for antibacterial photodynamic therapy (apdt) have received little attention due to their tendency to aggregate in aqueous media and reports of low phototoxic effect. these compounds may be less toxic to cells than positively and negatively charged photosensitisers. the preparation of highly bacterial phototoxic formulations of neutral porphyrins remains an open field of research with great potential if achievable. the purpose of this study was to develop novel hydrophilic for ... | 2015 | 25966307 |
| polyamine derivatives of betulinic acid and β-sitosterol: a comparative investigation. | β-sitosterol and betulinic acid were used in designing their conjugates with selected polyamines bearing either an amide bond, or an ester and an amide bond simultaneously in the target molecule. the synthesized compounds were subjected to basic cytotoxic and antimicrobial tests. the synthetic protocol is described separately for each of the three series of the target amides, because each series of compounds required a different synthetic approach. the cytotoxicity was tested on cells derived fr ... | 2015 | 25963549 |
| synthesis and evaluation of cationic norbornanes as peptidomimetic antibacterial agents. | a series of structurally amphiphilic biscationic norbornanes have been synthesised as rigidified, low molecular weight peptidomimetics of cationic antimicrobial peptides. a variety of charged hydrophilic functionalities were attached to the norbornane scaffold including aminium, guanidinium, imidazolium and pyridinium moieties. additionally, a range of hydrophobic groups of differing sizes were incorporated through an acetal linkage. the compounds were evaluated for antibacterial activity agains ... | 2015 | 25958967 |
| an antibacterial ortho-quinone diterpenoid and its derivatives from caryopteris mongolica. | to identify antibacterial components in traditional mongolian medicinal plant caryopteris mongolica, an ortho-quinone abietane caryopteron a (1) and three its derivatives caryopteron b-d (2-4) were isolated from the roots of the plant together with three known abietanes demethylcryptojaponol (5), 6α-hydroxydemethyl cryptojaponol (6), and 14-deoxycoleon u (7). the chemical structures of these abietane derivatives were elucidated on the basis of spectroscopic data. compounds 1-4 had c-13 methylcyc ... | 2015 | 25958242 |
| antibacterial properties associated with chitosan nanoparticle treatment on root dentin and 2 types of endodontic sealers. | the aim of this study was to evaluate the efficacy of carboxymethyl chitosan (cmcs) and chitosan nanoparticles (cnps) to inactivate bacteria and prevent biofilm formation at sealer-dentin interfaces. | 2015 | 25958178 |
| the effects of sodium hypochlorite and chlorhexidine irrigants on the antibacterial activities of alkaline media against enterococcus faecalis. | sodium hypochlorite (naocl), chlorhexidine (chx) and calcium hydroxide are common intracanal medicaments. the present study aimed to evaluate the effects of naocl and chx on the antibacterial activities of alkaline media against enterococcus faecalis. | 2015 | 25956995 |
| small hospitals matter: insights from the emergence and spread of vancomycin-resistant enterococci in 2 public hospitals in inner brazil. | although vancomycin-resistant enterococci (vre) are reported in brazil since 1996, data on their impact over settings of different complexity are scarce. we performed a study aimed at identifying determinants of vre emergence and spread in a public hospital consortium (comprising 2 hospitals, with 318 and 57 beds) in inner brazil. molecular typing and case-control studies (addressing predictors of acquisition or clonality) were performed. among 122 authocthonous isolates, 106 were enterococcus f ... | 2015 | 25956931 |
| daily variations in pathogenic bacterial populations in a monsoon influenced tropical environment. | changing climatic conditions have influenced the monsoon pattern in recent years. variations in bacterial population in one such tropical environment were observed everyday over two years and point out intra and inter annual changes driven by the intensity of rainfall. vibrio spp. were abundant during the monsoon and so were faecal coliforms. vibrio alginolyticus were negatively influenced by nitrate, whereas, silicate and rainfall positively influenced vibrio parahaemolyticus numbers. it is als ... | 2015 | 25956443 |
| the combination of ampicillin plus ceftaroline is synergistic against enterococcus faecalis. | 2015 | 25953804 | |
| effect of nacl treatments on tyramine biosynthesis of enterococcus faecalis. | the effect of nacl stress (0 to 8%, wt/vol) on the growth and tyramine production in two enterococcus faecalis strains was examined during culture time. the growth of e. faecalis was inhibited by the increase in nacl concentration, but tyramine production was unaffected. tyramine accumulated rapidly during the logarithmic phase of the strains, and the final tyramine levels were approximately 800 μg/ml. relative gene expression of four genes in the tyrosine decarboxylase locus, namely, tyrrs, tyr ... | 2015 | 25951388 |
| effect of apically separated rotary instruments on bacterial leakage of obturated root canals using resilon. | the purpose of this study was to determine the effect of a separated rotary instrument on the time required for bacterial penetration of obturated root canals using resilon. eighty-four extracted human maxillary premolars with single canals were used in this study. group 1 consisted of teeth obturated with gutta-percha and ah-26 sealer (n=15); group 2 consisted of teeth obturated similarly to group 1 but without a separated file; group 3 consisted of teeth obturated with resilon and epiphany sea ... | 2015 | 25928974 |
| genotypic variations of virulent genes in enterococcus faecium and enterococcus faecalis isolated from three hospitals in malaysia. | the genus enterococcus is of increasing significance as a cause of nosocomial infections, and this trend is exacerbated by the development of antibiotic resistance. | 2015 | 25923096 |
| multiple roles for enterococcus faecalis glycosyltransferases in biofilm-associated antibiotic resistance, cell envelope integrity, and conjugative transfer. | the emergence of multidrug-resistant bacteria and the limited availability of new antibiotics are of increasing clinical concern. a compounding factor is the ability of microorganisms to form biofilms (communities of cells encased in a protective extracellular matrix) that are intrinsically resistant to antibiotics. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and also has the propensity to acquire resistance determinants via horizontal gene transfer. there is i ... | 2015 | 25918141 |
| effects of photodynamic therapy on enterococcus faecalis biofilms. | microbial biofilms are involved in almost all infectious pathologies of the oral cavity. this has led to the search for novel therapies specifically aimed at biofilm elimination. in this study, we used atomic force microscopy (afm) to visualize injuries and to determine surface roughness, as well as confocal laser scanning microscopy (clsm) to enumerate live and dead bacterial cells, to determine the effects of photodynamic therapy (pdt) on enterococcus faecalis biofilms. the afm images showed t ... | 2015 | 25917515 |
| enterococcus faecalis gelatinase mediates intestinal permeability via protease-activated receptor 2. | microbial protease-mediated disruption of the intestinal epithelium is a potential mechanism whereby a dysbiotic enteric microbiota can lead to disease. this mechanism was investigated using the colitogenic, protease-secreting enteric microbe enterococcus faecalis. caco-2 and t-84 epithelial cell monolayers and the mouse colonic epithelium were exposed to concentrated conditioned media (ccm) from e. faecalis v583 and e. faecalis lacking the gelatinase gene (gele). the flux of fluorescein isothio ... | 2015 | 25916983 |
| proteomic investigation of the response of enterococcus faecalis v583 when cultivated in urine. | enterococcus faecalis is a robust bacterium, which is able to survive in and adapt to hostile environments such as the urinary tract and bladder. in this label-free quantitative proteomic study based on maxquant lfq algorithms, we identified 127 proteins present in the secretome of the clinical vancomycin-resistant isolate e. faecalis v583 and we compared proteins secreted in the initial phase of cultivation in urine with the secretome during cultivation in standard laboratory medium, 2xyt. of t ... | 2015 | 25915650 |
| tyrosine decarboxylase activity of enterococci grown in media with different nutritional potential: tyramine and 2-phenylethylamine accumulation and tyrdc gene expression. | the ability to accumulate tyramine and 2-phenylethylamine by two strains of enterococcus faecalis and two strains enterococcus faecium was evaluated in two cultural media added or not with tyrosine. all the enterococcal strains possessed a tyrosine decarboxylase (tyrdc) which determined tyramine accumulation in all the conditions tested, independently on the addition of high concentration of free tyrosine. enterococci differed in rate and level of biogenic amines accumulation. e. faecalis ef37 a ... | 2015 | 25914676 |
| preparation, characterization and antibacterial properties of zno/kaoline nanocomposites. | this paper describes laboratory preparation, characterization and antibacterial activity testing of zno/kaoline composites. zno/kaoline composites with 50 wt.% of zno were laboratory prepared, dried at 105 °c and calcined at 500 °c. xrpd analysis revealed that thermal treatment caused the phase transformation of zn containing precursor into zno. scanning and transmission electron microscopy techniques were used for characterization of morphology of the prepared samples. a standard microdilution ... | 2015 | 25909474 |
| interplay between copper and zinc homeostasis through the transcriptional regulator zur in enterococcus faecalis. | by integrating the microarray expression data and a global e. faecalis transcriptional network we identified a sub-network activated by zinc and copper. our analyses indicated that the transcriptional response of the bacterium to copper and zinc exposure involved the activation of two modules, module i that contains genes implicated in zinc homeostasis, including the zur transcriptional repressor, and module ii containing a set of genes associated with general stress response and basal metabolis ... | 2015 | 25906431 |
| ph-induced change in cell susceptibility to butanol in a high butanol-tolerant bacterium, enterococcus faecalis strain cm4a. | though butanol is considered as a potential biofuel, its toxicity toward microorganisms is the main bottleneck for the biological butanol production. recently, butanol-tolerant bacteria have been proposed as alternative butanol production hosts overcoming the end product inhibition. one remaining key issue to be addressed is how physicochemical properties such as ph and temperature affect microbial butanol tolerance during cultivation and fermentation. | 2015 | 25904984 |
| genotypic and phenotypic diversity in enterococcus faecalis: is agar invasion a pathogenicity score? | the main objective of the present study is to analyze different genotypic and phenotypic traits related to virulence in enterococcus faecalis, as well as evaluated the agar invasion phenotype in a collection of isolates with different clinical origins. | 2015 | 25904518 |
| competition between vanu(g) repressor and vanr(g) activator leads to rheostatic control of vang vancomycin resistance operon expression. | enterococcus faecalis bm4518 is resistant to vancomycin by synthesis of peptidoglycan precursors ending in d-alanyl-d-serine. in the chromosomal vang locus, transcription of the resistance genes from the pyg resistance promoter is inducible and, upstream from these genes, there is an unusual three-component regulatory system encoded by the vanurs(g) operon from the p(ug) regulatory promoter. in contrast to the other van operons in enterococci, the vang operon possesses the additional vanu(g) gen ... | 2015 | 25898178 |
| a variable dna recognition site organization establishes the liar-mediated cell envelope stress response of enterococci to daptomycin. | liar is a 'master regulator' of the cell envelope stress response in enterococci and many other gram-positive organisms. mutations to liar can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. liar is phosphorylated in response to membrane stress to regulate downstream target operons. using dna footprinting of the regions upstream of the liaxyz and liafsr operons we show that liar binds an extended stretch of dna that extends beyond the propos ... | 2015 | 25897118 |
| urinary tract infections and antimicrobial sensitivity among diabetic patients at khartoum, sudan. | patients with diabetes mellitus (dm) are more susceptible to urinary tract infection (uti) than non-diabetics. due to the emergence of multidrug resistant (mdr) uropathogenic strains, the choice of antimicrobial agent is restricted. this study investigated the epidemiology of uti, antimicrobial susceptibility, and resistance patterns of bacterial isolates from adult diabetic patients. | 2015 | 25896611 |
| total and specific bacterial levels in the apical root canal system of teeth with post-treatment apical periodontitis. | most studies of the microbiota in root canal-treated teeth focused only on the main canal, not distinguishing regions nor incorporating the intricate anatomy in the analysis. moreover, most of them provided only prevalence data. this study was designed to evaluate the total bacterial counts and the presence, levels, and relative abundance of candidate endodontic pathogens exclusively in the apical root canal system associated with post-treatment apical periodontitis. | 2015 | 25892512 |
| minimizing concentration of sodium hypochlorite in root canal irrigation by combination of ultrasonic irrigation with photodynamic treatment. | concentration of sodium hypochlorite (naocl) is positively correlated with its effectiveness in root canal disinfection but negatively correlated with its biocompatibility. the objective of this in vitro study was to compare the bactericidal effects among ultrasonic irrigation with different concentration of naocl alone or together with photodynamic treatment (pdt) against enterococcus faecalis (e. faecalis) in infected root canals. one hundred and twenty bovine root canals contaminated with e. ... | 2015 | 25892274 |
| micropan: an r-package for microbial pan-genomics. | a pan-genome is defined as the set of all unique gene families found in one or more strains of a prokaryotic species. due to the extensive within-species diversity in the microbial world, the pan-genome is often many times larger than a single genome. studies of pan-genomes have become popular due to the easy access to whole-genome sequence data for prokaryotes. a pan-genome study reveals species diversity and gene families that may be of special interest, e.g because of their role in bacterial ... | 2015 | 25888166 |
| evaluation of residual antibacterial effect of human radicular dentin treated with triple and double antibiotic pastes. | the aim of this study was to investigate the residual antibacterial effect of human radicular dentin treated with various concentrations of triple antibiotic paste and double antibiotic paste (dap). | 2015 | 25887806 |
| comprehensive molecular, genomic and phenotypic analysis of a major clone of enterococcus faecalis mlst st40. | enterococcus faecalis is a multifaceted microorganism known to act as a beneficial intestinal commensal bacterium. it is also a dreaded nosocomial pathogen causing life-threatening infections in hospitalised patients. isolates of a distinct mlst type st40 represent the most frequent strain type of this species, distributed worldwide and originating from various sources (animal, human, environmental) and different conditions (colonisation/infection). since enterococci are known to be highly recom ... | 2015 | 25887115 |
| investigating the mobilome in clinically important lineages of enterococcus faecium and enterococcus faecalis. | the success of enterococcus faecium and e. faecalis evolving as multi-resistant nosocomial pathogens is associated with their ability to acquire and share adaptive traits, including antimicrobial resistance genes encoded by mobile genetic elements (mges). here, we investigate this mobilome in successful hospital associated genetic lineages, e. faecium sequence type (st)17 (n=10) and st78 (n=10), e. faecalis st6 (n=10) and st40 (n=10) by dna microarray analyses. | 2015 | 25885771 |
| genetic relatedness and risk factor analysis of ampicillin-resistant and high-level gentamicin-resistant enterococci causing bloodstream infections in tanzanian children. | while enterococci resistant to multiple antimicrobials are spreading in hospitals worldwide, causing urinary tract, wound and bloodstream infections, there is little published data on these infections from africa. | 2015 | 25884316 |
| review and phylogenetic analysis of qac genes that reduce susceptibility to quaternary ammonium compounds in staphylococcus species. | the qac genes of staphylococcus species encode multidrug efflux pumps: membrane proteins that export toxic molecules and thus increase tolerance to a variety of compounds such as disinfecting agents, including quaternary ammonium compounds (for which they are named), intercalating dyes and some antibiotics. in stapylococcus species, six different plasmid-encoded qac efflux pumps have been described, and they belong to two major protein families. qaca and qacb are members of the major facilitator ... | 2015 | 25883793 |
| iatrogenic aortic insufficiency following mitral valve replacement: case report and review of the literature. | we report a 28-year-old white female who suffered significant aortic insufficiency (ai) following mitral valve (mv) replacement for endocarditis. the patient had history of rheumatoid arthritis and presented to our emergency department with a 3-month history of dyspnea, orthopnea, fevers and weight loss, worsening over 2 weeks, for which she took intermittent acetaminophen. on admission, vital signs revealed blood pressure of 99/70 mm hg, heart rate of 120 beats/minute, and temperature of 98.8 ° ... | 2015 | 25883714 |
| antibacterial and cytotoxic activity from the extract and fractions of a marine derived bacterium from the streptomyces genus. | marine natural products are a rich source of potent, selective, and structurally novel compounds. marine bacteria are considered the most promising source of biologically active compounds which can be applied to treat a wide range of diseases. | 2015 | 25880141 |
| prevalence, seasonality, and growth of enterococci in raw and pasteurized milk in victoria, australia. | this study investigated the prevalence, seasonality, and species variety of enterococci present in raw milk factory silos and pasteurized milk in 3 dairying regions in victoria, australia, over a 1-yr period. additionally, the growth ability of thermoduric enterococci isolated in this study (enterococcus faecalis, e. faecium, e. hirae, and e. durans) was determined in milk at temperatures likely to occur during storage, transport, and distribution, and before domestic consumption (4 and 7°c). en ... | 2015 | 26476940 |
| antimicrobial effect against different bacterial strains and bacterial adaptation to essential oils used as feed additives. | the aim of this study was to evaluate the antimicrobial activity and determine the minimum bactericidal concentration (mbc) of the essential oils derived from origanum vulgare (oregano), melaleuca alternifolia (tea tree), cinnamomum cassia (cassia), and thymus vulgaris (white thyme) against salmonella typhimurium, salmonella enteritidis, escherichia coli, staphylococcus aureus and enterococcus faecalis. the study also investigated the ability of these different bacterial strains to develop adapt ... | 2015 | 26424908 |
| antimicrobial activity and ph of calcium hydroxide and zinc oxide nanoparticles intracanal medication and association with chlorhexidine. | to evaluate ph and antibacterial activity of pastes with calcium hydroxide [ca(oh)2] and zinc oxide (zno) microparticles (micro) or nanoparticles (nano) and association with 0.4% chlorhexidine against enterococcus faecalis. | 2015 | 26423497 |
| chemical composition and in vitro antibacterial activity of the essential oil of verbesina negrensis from the venezuelan andes. | the composition and antimicrobial activity of the essential oil from verbesina negrensis steyerm. leaves is reported. analysis was performed by gc/ms. major constituents were α-pinene (43.1%), α-humulene (13.8%), δ-cadinene (8.1%), limonene (4.6%) and bicyclogermacrene (4.2%). the essential oil showed in vitro activity against the gram-positive bacteria staphylococcus aureus atcc 25923 and enterococcus faecalis atcc 29212 (mic 500 and 350 μl/ml respectively). | 2015 | 26411037 |
| lipoteichoic acid from an enterococcus faecalis clinical strain promotes tnf-α expression through the nf-κb and p38 mapk signaling pathways in differentiated thp-1 macrophages. | to study the immune-inflammatory response and signaling mechanism of macrophages to purified enterococcus faecalis (e. faecalis) lipoteichoic acid (lta), intact lta was obtained from an e. faecalis clinical strain p25rc using the butanol method and hydrophobic interaction chromatography purification. the fractions containing lta were determined using phosphate detection. contaminations with lipopolysaccharide and proteins were excluded using the limulus amoebocyte lysate assay and sodium dodecyl ... | 2015 | 26405548 |
| comparative analysis of the orphan crispr2 locus in 242 enterococcus faecalis strains. | clustered, regularly interspaced short palindromic repeats and their associated cas proteins (crispr-cas) provide prokaryotes with a mechanism for defense against mobile genetic elements (mges). a crispr locus is a molecular memory of mge encounters. it contains an array of short sequences, called spacers, that generally have sequence identity to mges. three different crispr loci have been identified among strains of the opportunistic pathogen enterococcus faecalis. crispr1 and crispr3 are assoc ... | 2015 | 26398194 |
| surveillance of gram-positive cocci infections and drug resistance. | in this study, the prevalence of gram-positive cocci isolates and the characteristics of multiple drug resistances in patients were investigated. antibiotic resistances were determined in the clinical microbiology laboratory with the methodology of the clsi (2012). the software whonet5.4 and spss13.0 were used for statistical analysis. there were a total of 6211 gram-positive cocci isolates, comprised of 2255 (36.3%) coagulase (-) staphylococci, 1277 (20.6%) staphylococci aureus, 1109 (17.9%) en ... | 2015 | 26386666 |
| [construction of luxs gene knockout mutant of enterococcus faecalis]. | to construct quorum sensing luxs knockout mutants of enterococcus faecalis through homologous recombination. | 2015 | 26383557 |
| comparative evaluation of antimicrobial activity of hydroalcoholic extract of aloe vera, garlic, and 5% sodium hypochlorite as root canal irrigants against enterococcus faecalis: an in vitro study. | enterococcus faecalis are the most resistant and predominant microorganisms recovered from root canals of teeth where previous treatment has failed. over the past decade, interest in drugs derived from medicinal plants has markedly increased. in dentistry, phytomedicines has been used as an anti-inflammatory, antibiotic, analgesic, sedative, and also as an endodontic irrigant. in endodontics, because of the cytotoxic reactions of most of the commercial intracanal medicaments and their inability ... | 2015 | 26381627 |
| orthopedic implant-infections. incompetence of staphylococcus epidermidis, staphylococcus lugdunensis and enterococcus faecalis to invade osteoblasts. | septic failure is still the major complication of prosthetic implants. entering host cells, bacteria hide from host immune defenses, shelter from extracellular antibiotics, and cause chronic infection. staphylococcus aureus, the leading etiologic agent of orthopedic implant-infections, is able to enter bone cells and induce osteoblast apoptosis, osteoclast recruitment, and highly destructive osteomyelitis. staphylococcus epidermidis, staphylococcus lugdunensis and enterococcus faecalis are oppor ... | 2015 | 26378773 |
| in vitro studies of antibacterial and antifungal wound dressings comprising h2tio3 and sio2 nanoparticles. | the incidence rate of the infected and complex wound is established at approximately 40,000/1 million of the world's adult population. the aim of this study was to assess the efficiency of three novel types of wound dressings comprising sodium chloride, metatitanic acid and silicon dioxide nanoparticles. the study design was to prove their antimicrobial properties against the microorganisms most commonly causing wound infections. the study evaluated the antimicrobial effect of tested dressings o ... | 2015 | 26373173 |
| antibacterial, antifungal and antimycobacterial activities of some pyrazoline, hydrazone and chalcone derivatives. | twenty-seven previously reported chalcones and their pyrazoline and hydrazone derivatives as well as two further chalcones have been screened for their antimicrobial, antifungal and antimycobacterial activities against standard microbial strains and drug resistant isolates. the minimum inhibitory concentration (mic) value of each compound was determined by a two-fold serial microdilution technique. the compounds were found to possess a broad spectrum of antimicrobial activities with mic values o ... | 2015 | 26372110 |
| effects of pre-encapsulated and pro-encapsulated enterococcus faecalis on growth performance, blood characteristics, and cecal microflora in broiler chickens. | the effects of microencapsulation of enterococcus faecalis on the growth performance, antioxidant activity, immune function, and cecal microbiota in broilers were investigated. broilers (1-day-old) were assigned randomly as follows: 5 treatments, 5 replicate pens per treatment, and 20 broilers per pen. treatments included (1) a basal diet (con), (2) con + aureomycin (1 g/kg of diet) (ant), (3) con + free non-encapsulated probiotics (1 × 10(9) cfu/kg of diet) (free), (4) con + pro-encapsulated pr ... | 2015 | 26371326 |
| deletion of liar reverses daptomycin resistance in enterococcus faecium independent of the genetic background. | we have shown previously that changes in liafsr, a three-component regulatory system predicted to orchestrate the cell membrane stress response, are important mediators of daptomycin (dap) resistance in enterococci. indeed, deletion of the gene encoding the response regulator liar in a clinical strain of enterococcus faecalis reversed dap resistance (dap-r) and produced a strain hypersusceptible to antimicrobial peptides. since liafsr is conserved in enterococcus faecium, we investigated the rol ... | 2015 | 26369959 |
| chemical compositions and aroma evaluation of volatile oil from the industrial cultivation medium of enterococcus faecalis. | enterococcus faecalis is one of the major lactic acid bacterium (lab) species colonizing the intestines of animals and humans. the characteristic odor of the volatile oils obtained from both the liquid medium after incubation (mai) and liquid medium before incubation (mbi) in the cultivation process of e. faecalis was investigated to determine the utility of the liquid medium. in total, fifty-six and thirty-two compounds were detected in the volatile oils from the mai (mai oil) and mbi (mbi oil) ... | 2015 | 26369592 |
| inhibitory effect of gels loaded with a low concentration of antibiotics against biofilm formation by enterococcus faecalis and porphyromonas gingivalis. | we explored longitudinally the inhibitory effect of gels loaded with 1 mg/ml modified triple antibiotic paste (mtap) or double antibiotic paste (dap) against biofilm formation by enterococcus faecalis and porphyromonas gingivalis. methylcellulose-based antibiotic gels of mtap (ciprofloxacin, metronidazole and clindamycin) and dap (ciprofloxacin and metronidazole) were prepared at a concentration of 1 mg/ml. individually cultured e. faecalis and p. gingivalis bacterial suspensions were treated wi ... | 2015 | 26369485 |
| erratum to: investigating the mobilome in clinically important lineages of enterococcus faecium and enterococcus faecalis. | 2015 | 26369411 | |
| the effects of indoor and outdoor dust exposure on the growth, sensitivity to oxidative-stress, and biofilm production of three opportunistic bacterial pathogens. | within the last decade, many studies have highlighted the radical changes in the components of indoor and outdoor dust. for example, agents like automobile emitted platinum group elements and different kinds of organic phthalates and esters have been reported to be accumulating in the biosphere. humans consistently face dermal, respiratory, and dietary exposures to these particles while indoors and outdoors. in fact, dust particulate matter has been associated with close to 500,000 deaths per ye ... | 2015 | 26363607 |
| purple urine bag syndrome in two elderly men with urinary tract infection. | purple urine bag syndrome is a rare condition in which purple discoloration of urine inside its collection bag occurs. we describe two illustrative cases. the first patient is an 81-year-old man who was hospitalized for a newly diagnosed lymphoma with acute obstructive renal failure for which a nephrostomy procedure was performed. during the hospitalization, a sudden purple discoloration of the suprapubic catheter urine was noted, while the nephrostomy urine had a normal color. urine culture fro ... | 2015 | 26351597 |
| the fibronectin-binding protein efba contributes to pathogenesis and protects against infective endocarditis caused by enterococcus faecalis. | efba is a pava-like fibronectin adhesin of enterococcus faecalis previously shown to be important in experimental urinary tract infection. here, we expressed and purified the e. faecalis og1rf efba and confirmed that this protein binds with high affinity to immobilized fibronectin, collagen i, and collagen v. we constructed an efba deletion mutant and demonstrated that its virulence was significantly attenuated (p < 0.0006) versus the wild type in a mixed inoculum rat endocarditis model. further ... | 2015 | 26351286 |
| enterococcus faecalis-related prostatitis successfully treated with moxifloxacin. | 2015 | 26349832 | |
| structure-activity relationship of the aminomethylcyclines and the discovery of omadacycline. | a series of novel tetracycline derivatives were synthesized with the goal of creating new antibiotics that would be unaffected by the known tetracycline resistance mechanisms. new c-9-position derivatives of minocycline (the aminomethylcyclines [amcs]) were tested for in vitro activity against gram-positive strains containing known tetracycline resistance mechanisms of ribosomal protection (tet m in staphylococcus aureus, enterococcus faecalis, and streptococcus pneumoniae) and efflux (tet k in ... | 2015 | 26349824 |
| antimicrobial resistance and virulence factor gene profiles of enterococcus spp. isolates from wild arctocephalus australis (south american fur seal) and arctocephalus tropicalis (subantarctic fur seal). | enterococci are natural inhabitants of the gastrointestinal tracts in humans and animals. epidemiological data suggest that enterococci are important reservoirs of antimicrobial resistant genes that may be transmitted from other bacterial species the aim of this study was to investigate the species composition, antimicrobial resistance and virulence genes in enterococci recovered from fecal samples of wild arctocephalus australis and a. tropicalis found dead along the south coast of brazil. from ... | 2015 | 26347323 |
| in mixed biofilms enterococcus faecalis benefits from a calcium hydroxide challenge and culturing. | to evaluate the fate of enterococcus faecalis in dual-species and multispecies biofilms after treatment with calcium hydroxide (ca(oh)2 ). | 2015 | 26333716 |
| antibiofilm efficacy of silver nanoparticles as a vehicle for calcium hydroxide medicament against enterococcus faecalis. | the aim of the present study was to investigate antibacterial characteristic and enterococcus faecalis (e. faecalis) biofilm suppression effect of different vehicles of calcium hydroxide as intracanal medicaments in short and long-term. | 2015 | 26327612 |
| characterization of two metal binding lipoproteins as vaccine candidates for enterococcal infections. | enterococcus faecium and faecalis are gram-positive opportunistic pathogens that have become leading causes of nosocomial infections over the last decades. especially multidrug resistant enterococci have become a challenging clinical problem worldwide. therefore, new treatment options are needed and the identification of alternative targets for vaccine development has emerged as a feasible alternative to fight the infections caused by these pathogens. | 2015 | 26322633 |
| enterococcal isolates carrying the novel oxazolidinone resistance gene optra from hospitals in zhejiang, guangdong, and henan, china, 2010-2014. | a collection of 1159 enterococcal isolates from five chinese hospitals were screened for the presence of the novel oxazolidinone resistance gene optra, which was found in 34 (2.9%) isolates. pulsed-field gel electrophoresis (pfge) typing of 29 optra-carrying enterococcus faecalis isolates revealed 25 pfge patterns, and multilocus sequence typing yielded 20 sequence types. routine surveillance of optra-positive enterococci in hospitals should be conducted to monitor and counteract their further d ... | 2015 | 26319902 |
| roles of tlr/myd88/mapk/nf-κb signaling pathways in the regulation of phagocytosis and proinflammatory cytokine expression in response to e. faecalis infection. | enterococcus faecalis is a commensal bacterium residing in the gastrointestinal tract of mammals, but in certain situations it is also an opportunistic pathogen which can cause serious disease. macrophages have been shown to play a critical role in controlling infections by commensal enterococci and also have an important role in mediating chromosomal instability and promoting colon cancer during high-level enterococcal colonization in genetically susceptible mice. however, the molecular mechani ... | 2015 | 26317438 |
| telavancin: mechanisms of action, in vitro activity, and mechanisms of resistance. | telavancin is a semisynthetic lipoglycopeptide derivative of vancomycin. telavancin has a dual mechanism of antibacterial action, disrupting peptidoglycan synthesis and cell membrane function. in 2014, the clinical and laboratory standards institute (clsi) revised the antimicrobial susceptibility testing method for telavancin, resulting in minimum inhibitory concentration (mic) determinations that are more accurate and reproducible and demonstrate greater in vitro potency than shown with the pre ... | 2015 | 26316559 |
| environmental waters as a source of antibiotic-resistant enterococcus species in belgrade, serbia. | despite the number of studies on antibiotic-resistant enterococci from serbian clinical settings, there are no data about environmental contamination with these bacteria. thus, this study investigated the prevalence of antibiotic-resistant enterococci in belgrade, serbia. enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance ... | 2015 | 26314345 |
| analysis of 5-year trends in daptomycin activity tested against staphylococcus aureus and enterococci from european and us hospitals (2009-2013). | this study evaluated daptomycin activity trends among meticillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococci (vre) over a 5-year period (2009-2013). consecutive, unique patient isolates of clinical significance were collected in 67 european (eu) (17 nations) and 145 us medical centres and were susceptibility tested in a central reference laboratory against daptomycin and various comparators by clsi broth microdilution methods. mic results were interpreted accordin ... | 2015 | 27873705 |
| aspirin plus ticlopidine prevented experimental endocarditis due to enterococcus faecalis and streptococcus gallolyticus. | enterococcus faecalis and streptococcus gallolyticus cause infective endocarditis (ie), which can originate from the continuous release or translocation of low bacterial numbers into the bloodstream. in this context, ie cannot be prevented with antibiotics. we previously demonstrated that aspirin plus ticlopidine protected rats from ie due to s. gordonii and staphylococcus aureus. here we showed that aspirin plus ticlopidine significantly reduced vegetation weight and protected 73 and 64% rats ( ... | 2015 | 26310867 |
| prevalence of multidrug resistant gram-positive cocci in a chinese hospital over an 8-year period. | gram-positive cocci are common causes of bloodstream and nosocomial infections, and their multi-drug resistance is an increasingly serious problem. the present study aimed to assess the prevalence of multi-drug-resistant gram-positive cocci in a chinese population. in this retrospective study, data about gram-positive cocci from in-patients (january 2006 and december 2013) at the second hospital of shanxi medical university, taiyuan, china, were reviewed. antimicrobial susceptibility profile of ... | 2015 | 26309609 |
| regulatory crosstalk between type i and type ii toxin-antitoxin systems in the human pathogen enterococcus faecalis. | we discovered a chromosomal locus containing 2 toxin-antitoxin modules (tas) with an antisense transcriptional organization in the e. faecalis clinical isolate v583. these tas are homologous to the type i txpa-rata system and the type ii mazef, respectively. we have shown that the putative mazf is toxic for e. coli and triggers rna degradation, and its cognate antitoxin maze counteracts toxicity. the second module, adjacent to mazef, expresses a toxin predicted to belong to the txpa type i famil ... | 2015 | 26305399 |
| correction: enterococcus faecalis glycolipids modulate lipoprotein-content of the bacterial cell membrane and host immune response. | 2015 | 26301715 | |
| unexpected abscess localization of the anterior abdominal wall in an adpkd patient undergoing hemodialysis. | autosomal dominant polycystic kidney disease (adpkd) is one of the most common monogenic disorders and the leading inheritable cause of end-stage renal disease worldwide. cystic and noncystic extrarenal manifestations are correlated with variable clinical presentations so that an inherited disorder is now considered a systemic disease. kidney and liver cystic infections are the most common infectious complications in adpkd patients. furthermore, it is well known that adpkd is commonly associated ... | 2015 | 26301109 |
| transcriptome analysis of enterococcus faecalis in response to alkaline stress. | enterococcus faecalis is the most commonly isolated species from endodontic failure root canals; its persistence in treated root canals has been attributed to its ability to resist high ph stress. the goal of this study was to characterize the e. faecalis transcriptome and to identify candidate genes for response and resistance to alkaline stress using illumina hiseq 2000 sequencing. we found that e. faecalis could survive and form biofilms in a ph 10 environment and that alkaline stress had a g ... | 2015 | 26300863 |
| dentin tubule invasion by enterococcus faecalis under stress conditions ex vivo. | enterococcus faecalis is the species most frequently isolated from failed endodontic treatments because it can survive under stress conditions imposed by root canal treatment. the objective of this study was to determine the ability of e. faecalis to invade dentine tubules under alkaline and energy-starvation stress and to explore the potential mechanisms. roots from single-rooted human teeth were infected with e. faecalis under alkaline and energy-starvation stress conditions. after 4 wk of cul ... | 2015 | 26296719 |
| adhesion potential of intestinal microbes predicted by physico-chemical characterization methods. | bacterial adhesion to epithelial surfaces affects retention time in the human gastro-intestinal tract and therefore significantly contributes to interactions between bacteria and their hosts. bacterial adhesion among other factors is strongly influenced by physico-chemical factors. the accurate quantification of these physico-chemical factors in adhesion is however limited by the available measuring techniques. we evaluated surface charge, interfacial rheology and tensiometry (interfacial tensio ... | 2015 | 26295945 |
| antimicrobial susceptibility patterns of enterococcus faecalis and enterococcus faecium isolated from poultry flocks in germany. | between 2010 and 2011, 145 enterococcus isolates (enterococcus faecalis, n = 127; enterococcus faecium, n = 18) were collected during routine bacteriologic diagnostics from broilers, layers, and fattening turkeys in germany showing various clinical signs. the susceptibility to 24 antimicrobial agents was investigated by broth microdilution test to determine minimum inhibitory concentrations (mics). all e. faecalis isolates (n = 127) were susceptible to the beta-lactam antibiotics ampicillin, amo ... | 2015 | 26292548 |
| effects of ozone and photo-activated disinfection against enterococcus faecalis biofilms in vitro. | the purpose of this study was to compare the antibacterial effects of gaseous ozone (o3) and photo-activated disinfection (pad) methods against enterococcus faecalis (e. faecalis) biofilms. | 2015 | 26289524 |
| [study on the antibacterial activity of four kinds of nano-hydroxyapatite composites against enterococcus faecalis]. | this study aims to compare and determine a kind of nano-hydroxyapatite composite material with good antibacterial efficacy on enterococcusfaecalis (e. faecalis) in vitro. | 2015 | 26281262 |
| cytotoxicity, genotoxicity and antibacterial effectiveness of a bioceramic endodontic sealer. | to compare the characteristics of bioceramic endodontic sealer endosequence bc sealer with those of ah plus sealer. | 2015 | 26281002 |
| sterilization of rotary niti instruments within endodontic sponges. | to determine whether the following can be sterilized by autoclaving - endodontic sponges, rotary nickel-titanium (niti) instruments within endodontic sponges, and rotary niti instruments with rubber stoppers. | 2015 | 26280766 |
| cryptogenic mycotic aneurysm of the superior mesenteric artery. | visceral artery aneurysms as a result of arterial degenerative disease are rare (0.1-2%), and superior mesenteric artery (sma) accounts for 3.2% of all reported series. however, mycotic sma aneurysms (smaas) are even rarer, and to the best of our knowledge, this is the first report of cryptogenic mycotic aneurysm of sma by enterococcus faecalis (ef). we report a case of 77-year-old man with 6-week history of supra pubic/left iliac fossa pain, weight loss and fever. the computed tomography demons ... | 2015 | 26276703 |
| investigations of the presence of caffeine in the rudawa river, kraków, poland. | caffeine concentration in surface water (rudawa river, kraków, poland) has been being investigated since 2011. the method applied for investigations was developed in 2011, and the first series of measurements of caffeine concentration in surface water began in 2011. caffeine concentration was determined by the gas chromatography-mass spectrometry (gc-ms) method. solid phase extraction (spe) was used to enrich the concentration of caffeine in water samples. as an internal standard, the caffeine i ... | 2015 | 26264791 |
| selective elimination of bacterial faecal indicators in the schmutzdecke of slow sand filtration columns. | slow sand filtration (ssf) is an effective low-tech water treatment method for pathogen and particle removal. yet despite its application for centuries, it has been uncertain to which extent pathogenic microbes are removed by mechanical filtration or due to ecological interactions such as grazing and competition for nutrients. in this study, we quantified the removal of bacterial faecal indicators, escherichia coli and enterococcus faecalis, from secondary effluent of a wastewater treatment plan ... | 2015 | 26264137 |
| an in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (mta) against five oral bacteria. | the purpose of this study was to evaluate the antibacterial ability of three mta (mta-angelus, endocem mta, and proroot mta) against five typical oral bacteria (streptococcus mutans, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus paracasei, and porphyromonas gingivalis). | 2015 | 26263538 |
| loss of antibiotic tolerance in sod-deficient mutants is dependent on the energy source and arginine catabolism in enterococci. | enterococci are naturally tolerant to typically bactericidal cell wall-active antibiotics, meaning that their growth is inhibited but they are not killed even when exposed to a high concentration of the drug. the molecular reasons for this extraordinary tolerance are still incompletely understood. previous work showed that resistance to killing collapsed specifically in mutants affected in superoxide dismutase (sod) activity, arguing that bactericidal antibiotic treatment led to induction of a s ... | 2015 | 26260456 |
| effect of starch source (corn, oats or wheat) and concentration on fermentation by equine faecal microbiota in vitro. | the goal was to determine the effect of starch source (corn, oats and wheat) and concentration on: (i) total amylolytic bacteria, group d gram-positive cocci (gpc), lactobacilli and lactate-utilizing bacteria, and (ii) fermentation by equine microbiota. | 2015 | 26255645 |
| a new uv-led device for automatic disinfection of stethoscope membranes. | stethoscopes are widely used by doctors and nurses. poor stethoscope hygiene is a potential source of nosocomial infection. this study aimed to propose an innovative solution, based on the latest advances in ultraviolet (uv) light-emitting diodes (leds), for disinfecting stethoscope membranes automatically and efficiently. | 2015 | 26254501 |
| in vitro activities of tedizolid and linezolid against gram-positive cocci associated with acute bacterial skin and skin structure infections and pneumonia. | tedizolid is a novel, expanded-spectrum oxazolidinone with potent activity against a wide range of gram-positive pathogens. a total of 425 isolates of gram-positive bacteria were obtained consecutively from patients with acute bacterial skin and skin structure infections (absssis) or pneumonia. these isolates included methicillin-susceptible staphylococcus aureus (mssa) (n = 100), methicillin-resistant staphylococcus aureus (mrsa) (n = 100), streptococcus pyogenes (n = 50), streptococcus agalact ... | 2015 | 26248355 |
| characterization of sviceucin from streptomyces provides insight into enzyme exchangeability and disulfide bond formation in lasso peptides. | lasso peptides are bacterial ribosomally synthesized and post-translationally modified peptides. they have sparked increasing interest in peptide-based drug development because of their compact, interlocked structure, which offers superior stability and protein-binding capacity. disulfide bond-containing lasso peptides are rare and exhibit highly sought-after activities. in an effort to expand the repertoire of such molecules, we heterologously expressed, in streptomyces coelicolor, the gene clu ... | 2015 | 26343290 |
| rapid, culture-independent, optical diagnostics of centrifugally captured bacteria from urine samples. | this work presents a polymeric centrifugal microfluidic platform for the rapid and sensitive identification of bacteria directly from urine, thus eliminating time-consuming cultivation steps. this "lab-on-a-disc" platform utilizes the rotationally induced centrifugal field to efficiently capture bacteria directly from suspension within a glass-polymer hybrid chip. once trapped in an array of small v-shaped structures, the bacteria are readily available for spectroscopic characterization, such as ... | 2015 | 26339318 |
| transfer, composition and technological characterization of the lactic acid bacterial populations of the wooden vats used to produce traditional stretched cheeses. | the biofilms of 12 wooden vats used for the production of the traditional stretched cheeses caciocavallo palermitano and pdo vastedda della valle del belìce were investigated. salmonella spp. and listeria monocytogenes were never detected. total coliforms were at low numbers with escherichia coli found only in three vats. coagulase-positive staphylococci (cps) were below the enumeration limit, whereas lactic acid bacteria (lab) dominated the surfaces of all vats. in general, the dominance was sh ... | 2015 | 26338114 |
| the influence of different peritoneal dialysis fluids on the in vitro activity of ampicillin, daptomycin, and linezolid against enterococcus faecalis. | intraperitoneal administration of antibiotics is recommended for the treatment of peritoneal dialysis-related peritonitis. however, little data are available on a possible interference between peritoneal dialysis fluids and the activity of antimicrobial agents. thus, the present in vitro study set out to investigate the influence of different peritoneal dialysis fluids on the antimicrobial activity of ampicillin, linezolid, and daptomycin against enterococcus faecalis. time-kill curves in four d ... | 2015 | 26337433 |
| antibacterial and residual antimicrobial activities against enterococcus faecalis biofilm: a comparison between edta, chlorhexidine, cetrimide, mtad and qmix. | we compared the antibacterial and residual antimicrobial activities of five root canal irrigants (17% edta,2% chlorhexidine,0.2% cetrimide, mtad, and qmix) in a model of enterococcus faecalis biofilm formation. sixty dentin blocks with 3-week e. faecalis biofilm were divided into six equal groups and flushed with irrigant for 2 min or left untreated. a blank control group was also established. antibacterial activities of the irrigants were evaluated by counting colony forming units. to test resi ... | 2015 | 26245711 |
| antimicrobial susceptibility profile of uropathogens in maluti adventist hospital patients, 2011. | urinary tract infections (utis) are amongst the most common infections encountered globally and are usually treated empirically based on bacterial resistance to antibiotics for a given region. unfortunately in lesotho, no published studies are available to guide doctors in the treatment of utis. treatment protocols for western countries have been adopted, which may not be applicable for this region. | 2015 | 26245603 |
| vancomycin-resistant enterococcal infections: epidemiology, clinical manifestations, and optimal management. | since its discovery in england and france in 1986, vancomycin-resistant enterococcus has increasingly become a major nosocomial pathogen worldwide. enterococci are prolific colonizers, with tremendous genome plasticity and a propensity for persistence in hospital environments, allowing for increased transmission and the dissemination of resistance elements. infections typically present in immunosuppressed patients who have received multiple courses of antibiotics in the past. virulence is variab ... | 2015 | 26244026 |