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diversity of ctx-m beta-lactamases and their promoter regions from enterobacteriaceae isolated in three parisian hospitals.nine clinical isolates of enterobacteriaceae (six escherichia coli and three proteus mirabilis) isolated in three parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. ctx-m-1, ctx-m-2, ctx-m-9, ctx-m-14 and two novel plasmid-mediated ctx-m beta-lactamases (ctx-m-20, and ctx-m-21) were identified by polymerase chain reaction and isoelectric focusing (pi>8) and ...200212007800
ceftriaxone activity against gram-positive and gram-negative pathogens isolated in us clinical microbiology laboratories from 1996 to 2000: results from the surveillance network (tsn) database-usa.ceftriaxone was introduced into clinical practice in the usa in 1985 and was the first extended-spectrum (third-generation) cephalosporin approved for once-daily treatment of patients with gram-positive or gram-negative infections. review of ceftriaxone activity is important given its continued use since the mid-1980s and reports of emerging resistance among all antimicrobial agent classes. we reviewed the activity of ceftriaxone and relevant comparative agents against five gram-positive and 11 ...200212007850
[profile and sensitivity to antibiotics of 2063 uropathogenic bacteria isolated in the center of tunisia].current bacteriological data are very useful when making therapeutic decisions in cases of non complicated urinary tract infection. in this article, we present the data gathered by a university hospital laboratory in mid-tunisia as well as the results of 17,829 urinary cytobacteriological examinations conducted in a multidisciplinary hospital during the year 2000. urine was sowed on usual agar. all bacterium cultivating at least 10(5) bacteria reported to ml and at 37 degrees c in a normal atmos ...200212012968
chromosome-encoded class d beta-lactamase oxa-23 in proteus mirabilis.ten nonrepetitive proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of clermont-ferrand, france, produced class d carbapenemase oxa-23. mics of imipenem were 0.25 to 0.5 microg/ml for these clinical isolates. molecular typing revealed that the 10 p. mirabilis isolates originated from the same clonal strain. hybridization of i-ceui-generated chromosome fragments with a bla(oxa-23) probe showed that the gene was chromosome encoded in the p. mirab ...200212019126
effect of tumor necrosis factor alpha, interferon gamma, and interleukin-4 on bacteria-enterocyte interactions.little is known about the mechanisms involved in bacterial translocation from the intestinal lumen to extraintestinal sites. because the cytokine cascade associated with sepsis, inflammation, and trauma has been shown to affect intestinal epithelial permeability, experiments were designed to clarify the effects of selected cytokines on bacterial adherence to and internalization by cultured ht-29 and caco-2 enterocytes.200212020125
competitive advantages of caedibacter-infected paramecia.intracellular bacteria of the genus caedibacter limit the reproduction of their host, the freshwater ciliate paramecium. reproduction rates of infected strains of paramecia were significantly lower than those of genetically identical strains that had lost their parasites after treatment with an antibiotic. interference competition occurs when infected paramecia release a toxic form of the parasitic bacterium that kills uninfected paramecia. in mixed cultures of infected and uninfected strains of ...200212022275
a comparison of the performance of cystine lactose electrolyte deficient (cled) agar with oxoid chromogenic urinary tract infection (cuti) medium for the isolation and presumptive identification of organisms from urine.as part of the uk antimicrobial resistance strategy and action plan, the public health laboratory service (phls) is required to collect antibiotic susceptibility data so that resistance trends and patterns can be monitored. most laboratories report urine gram negative isolates, as "coliforms" according to morphological appearance, but without an acceptable identification system the antimicrobial surveillance data will be meaningless. commercially available identification systems tend to be expen ...200212101200
swarming-coupled expression of the proteus mirabilis hpmba haemolysin operon.the hpma haemolysin toxin of proteus mirabilis is encoded by the hpmba locus and its production is upregulated co-ordinately with the synthesis and assembly of flagella during differentiation into hyperflagellated swarm cells. primer extension identified a sigma(70) promoter upstream of hpmb that was upregulated during swarming. northern blotting indicated that this promoter region was also required for concomitant transcription of the immediately distal hpma gene, and that the unstable hpmba tr ...200212101306
morphine, the proteus of organic molecules.this feature article encapsulates the senior author's longstanding interests in opiate chemistry and attempts to place it within an historical context and against the backdrop of related work by others who have viewed morphine as one of the pinnacles of natural product synthesis. biomimetic and 'bioanalogous' routes to the morphine skeleton are discussed followed by approaches based on the elaboration of phenanthrene platforms. the latter include an asymmetric synthesis of ent-morphine developed ...200212109065
epidemiology and genetics of rheumatoid arthritis.the prevalence of rheumatoid arthritis (ra) is relatively constant in many populations, at 0.5-1.0%. however, a high prevalence of ra has been reported in the pima indians (5.3%) and in the chippewa indians (6.8%). in contrast, low occurrences have been reported in populations from china and japan. these data support a genetic role in disease risk. studies have so far shown that the familial recurrence risk in ra is small compared with other autoimmune diseases. the main genetic risk factor of r ...200212110146
the etiology of urinary tract infection: traditional and emerging pathogens.the microbial etiology of urinary infections has been regarded as well established and reasonably consistent. escherichia coli remains the predominant uropathogen (80%) isolated in acute community-acquired uncomplicated infections, followed by staphylococcus saprophyticus (10% to 15%). klebsiella, enterobacter, and proteus species, and enterococci infrequently cause uncomplicated cystitis and pyelonephritis. the pathogens traditionally associated with uti are changing many of their features, par ...200212113867
role of pten, a lipid phosphatase upstream effector of protein kinase b, in epithelial thyroid carcinogenesis.both benign and malignant thyroid disease are well-established components of cowden syndrome (cs), an autosomal dominant disorder characterized by multiple hamartomas and breast cancer that may be considered a phakomatosis. the susceptibility gene for cs is pten, a tumor suppressor gene on 10q23.3 that encodes a lipid phosphatase that lies upstream of protein kinase b (akt). interestingly, carney complex is also a phakomatosis where multiple endocrine neoplasias are prominent and thyroid cancer ...200212119278
emerging antimicrobial resistances among proteus mirabilis in europe: report from the mystic program (1997-2001). meropenem yearly susceptibility test information collection.resistance patterns that are currently problematic in europe can vary greatly within the same species over time, among various patient populations and among geographic regions on the same continent. the results from the meropenem yearly susceptibility test information collection (mystic) program, which monitors carbapenem resistance rates in institutions using meropenem, were used to determine resistance differences among proteus mirabilis. mic results from 688 p. mirabilis strains were classifi ...200212120879
antibiotic resistance of bacteria associated with community-acquired urinary tract infections in the southern area of the gaza strip.the aim of this study was to identify the microorganisms that cause "community-acquired" urinary tract infections among adults and to investigate their resistance to fourteen selected antimicrobial agents. the uropathogens identified in 121 positive midstream urine cultures from the 270 subjects included in this study were escherichia coli (57.9%), proteus species (9.9%), enterobacter species (7.4%), klebsiella species (6.6%), pseudomonas species (5.8%), staphylococcus saprophyticus (5.0%), ente ...200212120880
resistant pathogens in urinary tract infections.antimicrobial susceptibility of bacteria causing urinary tract infection (uti) has evolved over several decades as antimicrobial exposure has repeatedly been followed by emergence of resistance. older populations in the community, long-term care facilities, or acute care facilities have an increased prevalence of resistant bacteria isolated from uti. resistant isolates are more frequent in long-term care populations than the community. resistant isolates include common uropathogens, such as esch ...200212121518
dna gyrase and topoisomerase iv mutations associated with fluoroquinolone resistance in proteus mirabilis.mutations associated with fluoroquinolone resistance in clinical isolates of proteus mirabilis were determined by genetic analysis of the quinolone resistance-determining region (qrdr) of gyra, gyrb, parc, and pare. this study included the p. mirabilis type strain atcc 29906 and 29 clinical isolates with reduced susceptibility (mic, 0.5 to 2 microg/ml) or resistance (mic, > or =4 microg/ml) to ciprofloxacin. susceptibility profiles for ciprofloxacin, clinafloxacin, gatifloxacin, gemifloxacin, le ...200212121936
lomefloxacin versus ciprofloxacin in the treatment of chronic bacterial prostatitis.a total of 182 patients with chronic bacterial prostatitis (cbp) were recruited into this multicentre prospective, randomized clinical study. of these, 93 were treated orally with lomefloxacin (lomx) 400 mg once daily and 89 with ciprofloxacin (cipx) 500 mg twice daily for 4 weeks. at 5-9 days 90/90 vs 86/86, at 4-6 weeks 82/83 vs 82/82, at 3 months 80/79 vs 78/75, and at 6 months 78/75 vs 75/72 patients aging from 18 to 70 years were evaluable bacteriologically/clinically according to a modifie ...200212127707
reactivity to bacterial, fungal, and parasite antigens in patients with lymphedema and elephantiasis.both secondary infections and antifilarial immunity are thought to play roles in the development and progression of lymphedema. to investigate this issue, immune responses to a panel of bacterial, fungal, and parasite antigens were examined for women with lymphedema and elephantiasis (n = 28) and for women with no clinical evidence of lymphatic dysfunction who were either microfilaremic (mf+, n = 23) or microfilaria- and filarial antigen-negative (ag-, n = 24). the prevalence and intensity of de ...200212135288
vaccines for proteus mirabilis in urinary tract infection.proteus mirabilis is a documented cause of urinary tract infection (uti) in the complicated urinary tract. urease-mediated urea hydrolysis is responsible for both virulence of the organism and the ability to cause urolithiasis. a urease-negative mutant of p. mirabilis is unable to initiate stone formation and colonizes the kidney at a significantly lower rate. the considerable pathology caused by p. mirabilis warrants the development of a vaccine. we have initiated the advancement of vaccine stu ...200212135833
aetiology of chronic prostatitis.a total of 388 patients with symptoms of chronic prostatitis and inflammatory findings in expressed prostatic secretion (eps) or in a urine sample collected immediately after prostate massage, were examined over a 2 year period at the outpatient department for urogenital infections, university hospital for infectious diseases 'dr fran mihaljević', zagreb, croatia. the infective aetiology was determined in 276 (71.13%) patients. chlamydia trachomatis was the causative pathogen in 109 patients, tr ...200212135835
strategies for the control of catheter encrustation.two general strategies have been adopted to develop catheter materials that resist encrustaion by bacterial biofilms: (a) the incorporation of antimicrobial agents into the polymers and (b) the production of materials with surface properties which prevent the adherence of bacterial cells. our experience to develop non-adherent surfaces which abstracts design from nature is reported. compounds based on 2-methacryloloxyethylphosphorylcholine co-polymerised with long-chain alkyl methacrylates have ...200212135840
green fluorescent protein urea sensors. uropathogenic proteus mirabilis. 200212136763
identification of mrpi as the sole recombinase that regulates the phase variation of mr/p fimbria, a bladder colonization factor of uropathogenic proteus mirabilis.proteus mirabilis is a common cause of urinary tract infection (uti) in individuals with structural abnormalities or long-term catheterization. the expression of mannose-resistant/proteus-like (mr/p) fimbria is phase variable because of the inversion of a 251 bp dna fragment that carries the promoter for the mrp operon. previous studies have shown that mrpi, which is transcribed divergently from the mrp operon, encodes a recombinase capable of switching the orientation of this invertible element ...200212139630
community-acquired bacterial meningitis in adults: the epidemiology, timing of appropriate antimicrobial therapy, and prognostic factors.between january 1986 and december 1999, 109 adult patients with culture-proven community-acquired bacterial meningitis were identified at kaohsiung chang gung memorial hospital. to compare changes over time, the appearance of disease among our patients was divided into two equal time periods: an earlier time period (1986-1992) and a later time period (1993-1999). in this study, there was a decreasing proportion of community-acquired bacterial meningitis compared with nosocomial bacterial meningi ...200212140104
interaction of the atypical prokaryotic transcription activator flhd2c2 with early promoters of the flagellar gene hierarchy.the transcriptional activator flhd2c2 is the master regulator of bacterial flagellum biogenesis and swarming migration, activating the "early" class ii promoters of the large flagellar gene hierarchy. using primer extensions, band-shift assays, and enzymatic and chemical footprinting, we describe the binding of the flhd2c2 heterotetramer to the promoter regions of four class ii flagella operons, fliaz, flhba and the divergent flgamn and flgbcd(efghij). each of the promoter regions was bound by a ...200212144778
evaluation of a new etest for detecting metallo-beta-lactamases in routine clinical testing.several etest (ab biodisk, solna, sweden) gradient formats were developed for detection of metallo-beta-lactamases based on the reduction of imipenem (ip) or ceftazidime (tz) mics in the presence of edta or 2-mercaptopropionic acid (mpa). the etest metallo-beta-lactamase (etest mbl) strips consisted of a double-sided seven-dilution range of ip or tz (4 to 256 microg/ml) and ip or tz (1 to 64 microg/ml) overlaid with a constant concentration of edta or mpa. the prototype strips were evaluated on ...200212149325
epidemiology and prognosis of bacteremia: a 10-y study in a community hospital.in order to determine the epidemiology and factors influencing the outcome of adult bacteremia in a community hospital, episodes of significant bacteremia were recorded prospectively over a 10-y period (1989-98). the following variables were included: age, sex, etiology, acquisition and source of the bacteremia, risk factors, clinical manifestations, empirical antibiotic treatment and outcome. a total of 798 episodes of bacteremia were recorded (436 in males) and 185 (24%) were hospital-acquired ...200212160171
assessment of peo/ptmo multiblock copolymer/segmented polyurethane blends as coating materials for urinary catheters: in vitro bacterial adhesion and encrustation behavior.the effective long-term use of indwelling urinary catheters has often been hindered by catheter-associated infection and encrustation. in this study, the suitability of poly(ethylene oxide) (peo)-based multiblock copolymer/segmented polyurethane (spu) blends as coating materials for the commercial urinary catheters was assessed by measuring swellability, bacterial adhesion, and encrustation behavior. when exposed to pbs (ph 7.4), the blends absorbed a significant amount of water, which was propo ...200212162332
[bacteriologic examination of gallbladder contents].acute calculous (obstructive) cholecystitis develops as a consequence of cystic obstruction and obstruction of bile flow into choledochus. most often it is a result of impacted gallstones in hartman's pouch or the cystic duct. their direct pressure on gallbladder mucosa causes ischemia, necrosis and ulceration with consequential wall edema and obstructed venous flow. this mechanism is further increasing and spreading the inflammatory process. ulcerations may be that extensive, that mucosa is hig ...200212170867
primary cutaneous alveolar rhabdomyosarcoma of the perineum.primary alveolar rhabdomyosarcoma (rms) involving perineal skin is extremely rare, particularly in the infant age group. we report a case of an alveolar rms in a newborn with abnormal symmetrical perineal overgrowth, causing ambiguous morphologic structure of the genitalia. clinical and imaging studies were suggestive of proteus syndrome with lymphatic malformation. histologic examination of the mass showed cutaneous alveolar rms with areas of embryonal and pleomorphic rms features. multiple sup ...200212171502
[effectiveness of imipenem/cilastatin (tienam, msd) in treating complicated infections in urology].complicated urinary infections tend to eventuate in severe pyoseptic complications--bacteriuria, sepsis. the search for methods of fighting agents of urinary infections goes in the direction of perfection of already existing methods and in the direction of design of novel antibacterial drugs. in the middle 1980s the first carbapenem drug-imipenem--was proposed for urological clinical practice. mechanism of its action as that of the other beta-lactam antibiotics consists in impairment of synthesi ...200212180053
vaginal microbiota in healthy pregnant women and prenatal screening of group b streptococci (gbs).the microbiota of the lower female genital tract was evaluated from vaginal swabs obtained from 623 healthy pregnant women at gestation periods of 35-40 weeks. isolated and identified microorganisms were expressed as percentages of total samples. as expected, lactobacilli made up the dominant vaginal microbiota (70%). enterobacteriaceae, mainly escherichia coli, klebsiella spp. and proteus, were present in 38% of the samples, which might reflect the possible contamination of vaginal tract with r ...200212180785
[experimental study on bacteriostasis of chitosan and sodium hyaluronate].to compare the effect and coverage of bacteriostasis of chitosan and sodium hyaluronate.200212181792
[antibiotic properties of the pseudoalteromonas genus bacteria isolated from the black sea water and molluscs].antagonistic properties of 41 strains of alteromonas-like bacteria isolated from the black sea water and molluscs have been studied. being grown on the rich medium "b" for marine bacteria, 21% of strains have shown high antagonistic activity against phytopathogenic fungi; 6% of strains inhibited the growth of bacillus subtilis, proteus vulgaris and candida albicans. spectrum of antagonistic activity was essentially changed on synthetic "bm" medium with acetate, glutamate, alpha-alanine as a sing ...200212190034
characterization of flagella produced by clinical strains of stenotrophomonas maltophilia.stenotrophomonas maltophilia is an emerging nosocomial pathogen associated with opportunistic infections in patients with cystic fibrosis, cancer, and hiv. adherence of this organism to abiotic surfaces such as medical implants and catheters represents a major risk for hospitalized patients. the adhesive surface factors involved in adherence of these bacteria are largely unknown, and their flagella have not yet been characterized biochemically and antigenically. we purified and characterized the ...200212194767
[evaluation of the rapid lumi 'eiken' using chemiluminescent assay for rapid antimicrobial susceptibility testing].one hundred ninety eight clinical isolates, including enterobacteriaceae (70 strains), pseudomonas aeruginosa (20 strains), acinetobacter baumannii (10 strains), staphylococci (50 strains), enterococci (20 strains), streptococcus pneumoniae (15 strains) and haemophilus influenzae (13 strains) were tested for their antimicrobial susceptibilities by using the rapid lumi 'eiken' (rl). as a reference method, broth microdilution method according to the national committee for clinical laboratory stand ...200212199112
proteus syndrome and sudden cardiac arrest; beware of pulmonary embolism! 200212204468
intravitreal penetration of cefepime after systemic administration to humans.to investigate the penetration of cefepime (a fourth-generation cephalosporin) into the vitreous after single-dose intravenous administration to human subjects.200212207129
a case of purple urine bag syndrome (pubs). 200212212364
pathology of a mouse model of x-linked chronic granulomatous disease.a colony of knockout mice (gene designation cybb tm1) has been maintained at this institution for 5 years. these mice are lacking the b subunit of nadph oxidase and are susceptible to experimental infection with aspergillus fumigatus. the purpose of this study was to document the spontaneous diseases present in these mice which are a murine model of x-linked chronic granulomatous disease and to compare these lesions to those of chronic granulomatous disease in humans. lesions were documented in ...200212213046
first report of a parasitic copepod (pennella balaenopterae) infestation in a pinniped.an infestation by the parasitic copepod pennella balaenopterae was found in a stranded, 8-mo-old, female northern elephant seal (mirounga angustirostris). diagnosis was based on the finding of the cephalothoraxes of 14 adult female copepods from three subcutaneous sites. bacteria cultured from lesion exudate included arcanobacterium phocae, escherichia coli, edwardsiella tarda, an enterococcus sp., and proteus mirabilis. the lesions were drained and irrigated with chlorhexidine, and the seal was ...200212216795
development of an elisa using a recombinant 41 kda partial protein (p45n') for the detection of riemerella anatipestifer infections in ducks.riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. an indirect enzyme-linked immunosorbent assay (elisa) has been developed to facilitate early detection of r. anatipestifer infection in ducks. the antigen used was a recombinant 41 kda n-terminal fragment (rp45n') of a newly characterized r. anatipestifer potential surface protein, p45, which was expressed in escherichia coli as an n- ...200212220809
chemical constituents and antimicrobial activity of the essential oil of lantana xenica.the aim of this work was to evaluate the chemical composition of lantana xenica essential oil and its antimicrobial activity. the oil from the aerial parts of lantana xenica mold. (verbenacea) was obtained by steam distillation and analyzed by gas chromatography/mass spectrometry. the major constituent of the oil was (e)-caryophyllene (35.2 %), with minor amounts of gamma-cadinene (13.3 %), alpha-pinene (9.3 %), ocimene (9.2 %) and germacrene d (6.6 %). the antimicrobial assays showed that the e ...200212221608
[antimicrobial, echinococcidial and immunostimulating properties of the drug cheblin-sk-1].the antimicrobial properties of the drug cheblin-ck-1 (cck-1) were determined in mice intraabdominally inoculated with proteus mirabilis-4691 in a dose of 140-200 million daily cultured microbial bodies. its comparison agent was ampicillin. cck-1 was found to act as an antibiotic similar to ampicillin in its effects. the antimicrobial activity of cck-1 against staphylococcus aureus and escherichia coli isolated from the contents of echinococcal cysts from patients operated on was also establishe ...200212224264
extended-spectrum beta-lactamases.extended-spectrum beta-lactamases (esbls) were discovered in europe in the early 1980s after widespread use of broad-spectrum antibiotics. they are largely derivatives of 3 progenitor beta-lactamases that confer resistance to ampicillin in gram-negative bacteria and are now carried on plasmids. substitutions at the active enzyme site of these progenitor enzymes at single or multiple amino sites have resulted in altered substrate affinities for esbls. depending on the location of the substitution ...200212226798
[clinical and pathogenetic significance of proteus mirabilis antibodies in uveitis associated with joint lesions in children and adolescents].immunological studies in 95 children with uveitis associated with juvenile chronic arthritis revealed infection with proteus mirabilis in 14.7% cases, mainly in cases with reactive arthritis and juvenile rheumatoid arthritis (jra). a clear-cut relationship between p. mirabilis infection and severity of the disease was detected. in 85% children infected with p. mirabilis uveitis was associated with a rapid decrease of ocular functions and development of complications, one of the most frequent of ...200212226974
pathogenic effects of bacteria isolated from larvae of hylesia metabus crammer (lepidoptera: saturniidae).hylesia metabus larvae are susceptible to several pathogens indigenous to the area in which they are found. some larvae show symptoms characteristic of bacterial infection; they become flaccid and lethargic, and show a marked loss of appetite. we isolated and identified 29 bacterial strains from live, dead and experimentally infected h. metabus larvae, and evaluated their pathogenic activity. the bacteria which caused mortality in the larvae were: pseudomonas aeruginosa (60-93.3%), proteus vulga ...200212234536
antimicrobial and anti-inflammatory activity of folklore: mallotus peltatus leaf extract.since ages mallotus peltatus (geist) muell. arg. var acuminatus (euphorbiaceae) leaf and stem bark is used in folk medicine to cure intestinal ailments and skin infections. in several intestinal ailments, localized inflammation is of common occurrence and hence we have evaluated the antimicrobial as well as anti-inflammatory activity of m. peltatus leaf extract. the crude methanol extract of m. peltatus leaves was found to be active against staphylococcus aureus, staphylococcus saprophyticus, st ...200212242000
use of an electronic nose system for diagnoses of urinary tract infections.the use of volatile production patterns produced by bacterial contaminants in urine samples were examined using electronic nose technology. in two experiments 25 and 45 samples from patients were analysed for specific bacterial contaminants using agar culture techniques and the major uti bacterial species identified. these samples were also analysed by incubation in a volatile generation test tube system for 4-5 h. the volatile production patterns were then analysed using an electronic nose syst ...200212243908
neonatal proteus syndrome? 200212244562
histopathologic and bacteriologic evaluations of cellulitis detected in legs and caudal abdominal regions of turkeys.the objective of this study was to identify the causative agent of cellulitis in turkeys. eighteen flocks from nine producers were sampled at the local processing plant, and 37 birds with cellulitis on legs or caudal thoracic area were obtained. none of the 37 birds with cellulitis had lesions in other organs. on gross examination, lesions were categorized into two groups: cellulitis with unopened skin lesions (type a) and cellulitis with opened skin lesions (type b). histopathologically, cellul ...200211922333
emerging extended-spectrum beta-lactamases in proteus mirabilis.beta-lactamase production was detected in 147 (52%) of 282 consecutive nonduplicate proteus mirabilis isolates obtained over a 1-year period from the s. matteo hospital of pavia (northern italy). seventy isolates (48% of the beta-lactamase producers) were found to produce extended-spectrum beta-lactamases (esbls), identified as per-1 (first report in this species) and tem-52 in 52 and 18 isolates, respectively. analysis of clonal diversity of the esbl producers suggested different spreading patt ...200211923394
[use of the multi-component vp-4 vaccine to prevent acute respiratory diseases at preschool children's establishments].the multicomponent vp-4 vaccine from klebsiella pneumonia, staphyloccocus aureus, proteus vulgaris, escherichia coli antigens was used to prevent acute respiratory diseases (ard) at preschoolchildren's establishments. prevention was effected during an influenza epidemic with a drastic rise in the incidence of ard. the vp-4 use involved 3 nasal and 6-9 oral administrations of the drug at an interval of 3-4 days. the duration of the course was 26 +/- 4 days. the preventive action of vp-4 on the in ...200211924123
properties and utility of the peculiar mixed disulfide in the bacterial glutathione transferase b1-1.bacterial glutathione transferases appear to represent an evolutionary link between the thiol:disulfide oxidoreductase and glutathione transferase superfamilies. in particular, the observation of a mixed disulfide in the active site of proteus mirabilis glutathione transferase b1-1 is a feature that links the two families. this peculiar mixed disulfide between cys10 and one gsh molecule has been studied by means of esr spectroscopy, stopped-flow kinetic analysis, radiochemistry, and site-directe ...200211926831
staghorn calculus endotoxin expression in sepsis.staghorn calculi are infrequent and generally are infected stones. struvite or apatite calculi are embedded with gram-negative bacteria, which can produce endotoxin. sepsis syndrome may occur after surgical therapy or endoscopic manipulation of infected or staghorn calculi. sepsis, which can occur despite perioperative antibiotic use, may be due to bacteremia or endotoxemia. we present a child with an infected staghorn calculus who developed overwhelming sepsis and died after percutaneous stone ...200211927328
sensory, chemical and bacteriological changes in vacuum-packed pressurised squid mantle (todaropsis eblanae) stored at 4 degrees c.sensory, chemical and bacteriological changes were studied in vacuum-packed squid mantles (todaropsis eblanae) that were pressurised at 150, 200, 300 and 400 megapascal (mpa) for 15 min at ambient temperature and stored at 4 degrees c. sensory analysis showed that the higher the pressurisation the longer the shelf-life. thus, the lot pressurised at 400 mpa was rejected after 28 days' storage compared with 7 days for the untreated lot. the chemical results generally corresponded with the sensory ...200211929163
prevalence of extended spectrum beta-lactamase (esbl)-producing clinical isolates in the asia-pacific region and south africa: regional results from sentry antimicrobial surveillance program (1998-99).the frequency of occurrence of esbl-producing clinical strains varies widely in distinct geographic areas. the objective of this study was to describe the frequency of occurrence, the preferred substrate, and the co-resistance patterns of the esbl-producing isolates collected from the asia-pacific region and south africa through the sentry antimicrobial surveillance program between january 1998 and december 1999. a total of 1,377 escherichia coli, 678 klebsiella pneumoniae, and 138 proteus mirab ...200211929691
presence of rickettsia helvetica in granulomatous tissue from patients with sarcoidosis.in samples obtained during the autopsies of 2 patients with sarcoidosis, genetic material from rickettsia helvetica was detected by polymerase chain reaction, and histologic and immunohistochemical examination (using 3 different antibodies) of the polymerase chain reaction-positive tissues showed different degrees of granuloma formation and presence of rickettsia-like organisms predominantly located in the endothelium and macrophages. electron microscopic examination clearly identified and demon ...200211930323
evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies.phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ...200211931166
frequent association with neurosurgical conditions in adult proteus mirabilis meningitis: report of five cases.adult proteus (p.) mirabilis meningitis is relatively rare and has not been examined individually in the english-language literature. during a period of 15 years (january 1986-december 2000), four adult patients with p. mirabilis meningitis and one adult patient with mixed bacterial meningitis involving p. mirabilis were identified at chang gung memorial hospital, kaohsiung. these five patients included one man and four women, aged from 19 to 74 years (mean age=55.4). p. mirabilis infection acco ...200211932041
prevalence of extended spectrum beta-lactamases among enterobacteriaceae: an italian survey.the prevalence of extended-spectrum beta-lactamase (esbl) production by consecutive non-repeated isolates of enterobacteriaceae was determined over a 6-month period. a total of 8015 strains were isolated from ten italian laboratories and 509 (6.3%) of these were designated esbl producers from the results of a double-disk synergy test. escherichia coli was the most isolated microrganism, followed by klebsiella pneumoniae and proteus mirabilis. providencia stuartii (28.1%) was the most frequently ...200211932144
the m603 idiotype is lost in the response to phosphocholine in terminal deoxynucleotidyl transferase-deficient mice.the majority of anti-phosphocholine (pc) antibodies induced by the pc epitope in proteus morganii (pm) express the m603 idiotype (id), which is characterized by an invariant asp to asn substitution at the v(h):d(h) junction. to elucidate the molecular basis by which m603-like b cells acquire the mutations resulting in this invariant substitution, we analyzed the immune response to pc-pm in terminal deoxynucleotidyl transferase (tdt) gene knockout (ko) mice. in the absence of tdt, t15-id antibodi ...200211932921
crystals of tryptophan indole-lyase and tyrosine phenol-lyase form stable quinonoid complexes.the binding of substrates and inhibitors to wild-type proteus vulgaris tryptophan indole-lyase and to wild type and y71f citrobacter freundii tyrosine phenol-lyase was investigated in the crystalline state by polarized absorption microspectrophotometry. oxindolyl-lalanine binds to tryptophan indole-lyase crystals to accumulate predominantly a stable quinonoid intermediate absorbing at 502 nm with a dissociation constant of 35 microm, approximately 10-fold higher than that in solution. l-trp or l ...200211934889
precocious puberty in a female with proteus syndrome.proteus syndrome is a complex disorder comprising malformations and overgrowth of multiple tissues and characterized by its polymorphism and mosaicism. the syndrome is rare and sporadic. oliveira m da c et al reported the first case of proteus syndrome associated with precocious puberty in a boy. we are reporting a case of a 7-month old girl with proteus syndrome who developed a juvenile granulosa cell tumor in one ovary causing precocious puberty. at our knowledge this is the first case of prot ...200211938428
glutamic acid-65 is an essential residue for catalysis in proteus mirabilis glutathione s-transferase b1-1.the functional role of three conserved amino acid residues in proteus mirabilis glutathione s-transferase b1-1 (pmgst b1-1) has been investigated by site-directed mutagenesis. crystallographic analyses indicated that glu(65), ser(103) and glu(104) are in hydrogen-bonding distance of the n-terminal amino group of the gamma-glutamyl moiety of the co-substrate, gsh. glu(65) was mutated to either aspartic acid or leucine, and ser(103) and glu(104) were both mutated to alanine. glu(65) mutants (glu(6 ...200211903062
structure of the core part of the lipopolysaccharides from proteus penneri strains 7, 8, 14, 15, and 21.the core-lipid a region of the lipopolysaccharides from proteus penneri strains 7, 8, 14, 15, and 21 was studied using nmr spectroscopy, esi ms, and chemical analysis after alkaline deacylation, deamination, and mild-acid hydrolysis of the lipopolysaccharides. the following general structure of the major core oligosaccharides is proposed: [abstract: see text] where all sugars are in the pyranose form and have the d configuration unless otherwise stated, hep and ddhep=l-glycero- and d-glycero-d-m ...200211909598
proteus syndrome in adulthood.proteus syndrome is a very rare congenital condition comprising malformations and overgrowth of multiple sorts of tissue. it was described for the first time in 1979 and was termed proteus syndrome in 1983. the authors describe a 37-year-old patient who was diagnosed initially as having klippel-trenaunay-weber syndrome at the age of 10 years. the patient was operated for a major thoracic lymphatic malformation, which caused functional problems. the operation at older age for this large lymphatic ...200211910228
a transcription terminator in the groex gene of symbiotic x-bacteria expressed in escherichia coli.the over-expressing groex gene of symbiotic x-bacteria in amoeba proteus has unique nucleotide motifs (tx), containing two hairpins and a c-rich region at its 3'-end. to investigate the role of tx as a transcription terminator, we mutated tx and analyzed the effects on the expression of an upstream-located lacz in e. coli. the level of beta-galactosidase (beta-gal) production in tx deletion mutants was reduced to 23% of the control level. site-directed mutation of the hairpin-1, c-rich region, a ...200211911472
[four cases of non-clostridial gas gangrene with diabetes mellitus].we report four cases of non-clostridial gas gangrene. all cases were associated with diabetes mellitus as the underlying disease. case 1: a 60-year-old male developed an ulcerative lesion on the dorsum of his left foot. peptostreptococcus asaccharolyticus, citrobacter freundii and staphyrococcus epidermidis were identified in culture from odoriferous pus. case 2: a 81-year-old female developed a lesion on her vulva spreading to the right lower abdomen. bacteroides bivius, peptostreptococcus asac ...200211915237
structure of an extended-spectrum class a beta-lactamase from proteus vulgaris k1.the structure of a chromosomal extended-spectrum beta-lactamase (esbl) having the ability to hydrolyze cephalosporins including cefuroxime and ceftazidime has been determined by x-ray crystallography to 1.75 a resolution. the species-specific class a beta-lactamase from proteus vulgaris k1 was crystallized at ph 6.25 and its structure solved by molecular replacement. refinement of the model resulted in crystallographic r and r(free) of 16.9 % and 19.3 %, respectively. the folding of the k1 enzym ...200211916382
[identification of plasmid-encoded cephalosporinase acc-1 among various enterobacteria (klebsiella pneumoniae, proteus mirabilis, salmonella) isolated from a tunisian hospital (sfax 997-2000)].because a multiresistant k. pneumoniae outbreak detected in an intensive care unit of a parisian hospital, combined to the production of the plasmid-encoded cephalosporinase acc-1, a probable importation via a patient was suggested from another country (tunisia). the investigation was conducted to examine 35 clinical strains of enterobacteria resistant to ceftazidime without synergy towards augmentin. other test of synergy with two inhibitors, brl 42715, ro 48-5545 was performed by diffusion met ...200211873633
structure of the o-polysaccharide and classification of proteus mirabilis strain g1 in proteus serogroup o3.the o-chain polysaccharide of the lipopolysaccharide (lps) of a previously nonclassified strain of proteus mirabilis termed g1 was studied by sugar analysis and 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, rotating-frame noe (roesy), h-detected 1h,13c hmqc, and heteronuclear multiple-bond correlation (hmbc) experiments. the following structure of the polysaccharide was established: [carbohydrate structure: see text] where d-gala6(l-lys) stands for n(alpha)-(d-galacturonoyl)-l-lysine. t ...200211874454
protean pten: form and function.germline mutations distributed across the pten tumor-suppressor gene have been found to result in a wide spectrum of phenotypic features. originally shown to be a major susceptibility gene for both cowden syndrome (cs), which is characterized by multiple hamartomas and an increased risk of breast, thyroid, and endometrial cancers, and bannayan-riley-ruvalcaba syndrome, which is characterized by lipomatosis, macrocephaly, and speckled penis, the pten hamartoma tumor syndrome spectrum has broadene ...200211875759
craniofacial and dental manifestations of proteus syndrome: a case report.the proteus syndrome is a rare congenital hamartomatous condition that is characterized by a wide range of malformations, sometimes involving the face. common manifestations include partial gigantism, congenital lipomas, and plantar hyperplasia. in this report we describe the craniofacial clinicopathological development in a girl with proteus syndrome from age 6 to 20 years. the patient had pronounced hemifacial hypertrophy, exostoses in the left parietal region, and enlargement of the inferior ...200211879083
treatment of chronic suppurative otitis media with ofloxacin in hydroxypropyl methylcellulose ear drops: a clinical/bacteriological study in a rural area of malawi.chronic suppurative otitis media in young children is a major problem in africa, with socio-economic consequences at a later age. common treatment regimens with antibiotics are expensive and often not practically feasible. therefore, a project was started to develop a low-cost and effective treatment in a rural area of malawi by studying the clinical efficacy of an inexpensive application regimen of ofloxacin (0.075%) in hydroxypropyl methylcellulose (1.5%) ear drops. in earlier studies with thi ...200211879929
single-dose fluoroquinolone therapy of acute uncomplicated urinary tract infection in women: results from a randomized, double-blind, multicenter trial comparing single-dose to 3-day fluoroquinolone regimens.to compare the efficacy and safety of single-dose and 3-day fluoroquinolone treatment of uncomplicated urinary tract infection (uti).200211880065
detection and mutation of a luxs-encoded autoinducer in proteus mirabilis.quorum sensing regulates the expression of virulence factors in a wide variety of pathogenic bacteria. this study has shown that proteus mirabilis harbours a homologue of luxs, a gene required for the synthesis of the quorum sensing autoinducer 2 (ai-2). ai-2 activity is expressed during and is correlated with the initiation of swarming migration on agar surfaces. the p. mirabilis luxs locus was cloned and a luxs(-) strain constructed by allelic-exchange mutagenesis. while lacking ai-2 activity, ...200211882712
proteus syndrome.proteus syndrome is a rarely described dysplasia syndrome of the group of congenital hamartomas that arises from mosaic mutation. an extraordinary case history including imaging studies will be reported. this 17-year-old girl suffered from cachexia, lifelong chronic obstipation, different dysplasias, and lipomatous tumor-like lesions. the following findings were marked: macrodactyly, nevi, hemihypertrophy, aggressive lipomatosis, hemangiomas of the spleen, and skull and cerebral malformations. a ...200211884784
novel bacterial membrane surface display system using cell wall-less l-forms of proteus mirabilis and escherichia coli.we describe a novel membrane surface display system that allows the anchoring of foreign proteins in the cytoplasmic membrane (cm) of stable, cell wall-less l-form cells of escherichia coli and proteus mirabilis. the reporter protein, staphylokinase (sak), was fused to transmembrane domains of integral membrane proteins from e. coli (lactose permease lacy, preprotein translocase secy) and p. mirabilis (curved cell morphology protein ccma). both l-form strains overexpressed fusion proteins in amo ...200211823186
molecular characterization of extended-spectrum beta-lactamases produced by nosocomial isolates of enterobacteriaceae from an italian nationwide survey.extended-spectrum beta-lactamases (esbls) are widespread in hospital settings worldwide. the present investigation was undertaken to assess the distribution and prevalence of esbls belonging to the tem and shv families in 448 esbl-producing clinical isolates of enterobacteriaceae collected from 10 different italian hospitals. the natures of tem and shv determinants were identified by direct sequencing of pcr-amplified genes. tem-52 and shv-12 were the most common variants, and they were found in ...200211825979
structural and serological relatedness of the o-antigens of proteus penneri 1 and 4 from a novel proteus serogroup o72.o-specific polysaccharides (o-antigens) of the lipopolysaccharides (lps) of proteus penneri strains 1 and 4 were studied using sugar analysis, (1)h and (13)c nmr spectroscopy, including 2d cosy, h-detected (1)h,(13)c hmqc, and rotating-frame noe spectroscopy (roesy). the following structures of the tetrasaccharide (strain 1) and pentasaccharide (strain 4) repeating units of the polysaccharides were established: [reaction: see text]. in the polysaccharide of p. penneri strain 4, glycosylation wit ...200211784330
the characterization of bacterial isolates from acute otitis media in ile-ife, southwestern nigeria.the incidence of acute otitis media (aom) in a comprehensive healthcare setting was investigated in ile-ife, nigeria. out of the 617 children examined, 53 (11.64 per cent) suffered from the condition based on the criteria used. staphylococci constituted the predominant organisms associated with the condition with staphylococcus aureus (25.0 per cent) being the most frequent single microbe recovered from the subjects. this was followed by proteus mirabilis (16.2 per cent), staphylococcus sp. (8.8 ...200211866331
rapid antimicrobial susceptibility testing of urinary tract isolates and samples by flow cytometry.a multiparametric flow cytometry antimicrobial susceptibility test was developed and its performance was evaluated on clinical urine isolates and samples in comparison with standard methods. alterations in cytoplasmic membrane integrity were monitored by propidium iodide, and the anionic probe bis-(1,3-dibutylbarbituric acid) trimethine oxonol (dibac4(3)) was used to measure changes in membrane potential. microbial size and cellular content were analysed by light scattering. twelve antibiotics w ...200211871613
[in vitro antimicrobial activity of glutaraldehyde plus o-phenylphenol association (ratio 2:1)].the correct use of antiseptics, disinfectants and sterilization processes to inactivate or remove micro-organisms is an essential component of an effective infection control program. to reach this result the use of phenolic detergent-disinfectants which work well as a presoak has always been suggested in lieu of sterilization involving submerging instruments in a properly prepared glutaraldehyde solution for about 6 to 10 hrs. this study investigated the in vitro effectiveness of disinfectant so ...200211845118
current presentation and optimal surgical management of sternoclavicular joint infections.infection of the stemoclavicular joint is unusual, and treatment of this entity has not been standardized. we sought to characterize the current presentation and optimal management of this disease.200211845854
the migration of proteus mirabilis and other urinary tract pathogens over foley catheters.to examine the ability of organisms that infect the catheterized urinary tract to migrate over the surfaces of foley catheters.200211849161
characterization of a new extended-spectrum beta-lactamase (tem-87) isolated in proteus mirabilis during an italian survey.a new natural tem derivative, named tem-87, was identified in a proteus mirabilis isolate from an italian hospital. compared to tem-1, tem-87 contains the following mutations: e104k, r164c, and m182t. kinetic analysis of tem-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. expression of blatem-87 in escherichia coli decreased the host susceptibility to these drugs.200211850290
genital tract tumors in proteus syndrome: report of a case of bilateral paraovarian endometrioid cystic tumors of borderline malignancy and review of the literature.proteus syndrome is a rare, sporadic disorder that causes postnatal overgrowth of multiple tissues in a mosaic pattern. characteristic manifestations include: overgrowth and hypertrophy of limbs and digits, connective tissue nevus, epidermal nevus and hyperostoses. various benign and malignant tumors and hamartomas may complicate the clinical course of patients with the syndrome. commonly encountered tumors include hemangiomas, lymphangiomas and lipomas. tumors of the genital tract occur less of ...200211850547
susceptibility to fluoroquinolones among commonly isolated gram-negative bacilli in 2000: trust and tsn data for the united states. tracking resistance in the united states today. the surveillance network.from january to may 2000, as part of the tracking resistance in the united states today (trust) surveillance initiative, clinical isolates of enterobacteriaceae (n=2519) and non-fermentative gram-negatives (n=580) were prospectively collected from 26 hospital laboratories across the united states. isolates were tested for susceptibility to three fluoroquinolones (ciprofloxacin, levofloxacin, gatifloxacin) and seven other agents. in addition, data for the same period were collected from the surve ...200211814764
occurrence of extended-spectrum beta-lactamases in members of the family enterobacteriaceae in italy: implications for resistance to beta-lactams and other antimicrobial drugs.an italian nationwide survey was carried out to assess the prevalences and the antimicrobial susceptibilities of members of the family enterobacteriaceae producing extended-spectrum beta-lactamases (esbls). over a 6-month period, 8,015 isolates were obtained from hospitalized patients and screened for resistance to extended-spectrum cephalosporins and monobactams. on the basis of a synergistic effect between clavulanate and selected beta-lactams (ceftazidime, aztreonam, cefotaxime, cefepime, and ...200211751134
postneurosurgical meningitis due to proteus penneri with selection of a ceftriaxone-resistant isolate: analysis of chromosomal class a beta-lactamase huga and its lysr-type regulatory protein hugr.we report on a case of a postneurosurgical meningitis due to ceftriaxone-susceptible proteus penneri, with selection of a ceftriaxone-resistant isolate following treatment with ceftriaxone. the isolates presented identical patterns by pulsed-field gel electrophoresis and produced a single beta-lactamase named huga with an isoelectric point of 6.7. the ceftriaxone-resistant isolate hyperproduced the beta-lactamase (increase in the level of production, about 90-fold). the sequences of the huga bet ...200211751137
solid-phase synthesis of new saphenamycin analogues with antimicrobial activity.an array of 12 new saphenamycin analogues modified at the benzoate moiety was synthesized on solid support. synthesis commenced with a chemoselective anchoring of saphenic acid through the carboxyl group to a 2-chlorotrityl functionalized polystyrene resin. the secondary alcohol was acylated in parallel with a series of differently substituted benzoic acid derivatives. treatment with tfa-ch(2)cl(2) (5:995) released the expected saphenamycin analogues into solution. these new analogues were purif ...200211755347
visualization of proteus mirabilis within the matrix of urease-induced bladder stones during experimental urinary tract infection.the virulence of a urease-negative mutant of uropathogenic proteus mirabilis and its wild-type parent strain was assessed by using a cba mouse model of catheterized urinary tract infection. overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type p. mirabilis. at a high inoculum, the urease-negative mutant successfully colonized bladders of catheterized mice but did not cause urolithiasis and was still severely attenuated in its ability to ...200211748205
invitro comparative evaluation of aminoglycosides at a cancer centre.a comparative study of in vitro activity of amikacin, gentamicin, netilmicin, tobramycin and isepamicin was done. a total of 200 clinical isolates of gram negative organisms from various clinical sources were tested. e.coli was the most frequently isolated organism followed by pseudomonas spp., klebsiella spp., proteus spp., acinetobacter spp. and enterobacter spp. of the 5 aminoglycosides tested, isepamicin showed the highest numbers of susceptible isolates followed by amikacin, netilmicin, gen ...200212928571
[cefepime (maxipime) treatment efficacy in surgical patients ].between september and december 2001 in the "caritas" surgical clinic of bucharest has been conducted a clinical study for the efficiency of cefepime (maxipime) treatment in surgical patients. introduced in therapy in the last decade of the xxth century, cefepime (maxipime) is the most active 4th generation cephalosporin, due to its extended spectrum of activity and its high resistance against beta-lactamases. cefepime (maxipime) has a very large spectrum, including the majority of the microorgan ...200212731215
extended spectrum beta-lactamase mediated resistance in urinary tract isolates of family enterobacteriaceae.extended spectrum beta-lactamases (esbl) hydrolyse expanded spectrum cephalosporins like ceftazidime, cefotaxime and monobactam aztreonam. esbl producing bacteria may not be detectable in the routine disk diffusion susceptibility tests leading to inappropriate use of antibiotics and treatment failures. as information on esbl producers causing urinary tract infection (uti) is not available from our country, the occurrence of esbl producing strains causing uti was studied as also the differences b ...200212674828
information extraction for enhanced access to disease outbreak reports.document search is generally based on individual terms in the document. however, for collections within limited domains it is possible to provide more powerful access tools. this paper describes a system designed for collections of reports of infectious disease outbreaks. the system, proteus-bio, automatically creates a table of outbreaks, with each table entry linked to the document describing that outbreak; this makes it possible to use database operations such as selection and sorting to find ...200212755518
aerobic pathogenic bacteria in post-operative wounds at moi teaching and referral hospital.the emergence of bacterial antimicrobial resistance associated with acquired infections has made the choice of empirical therapy more difficult and expensive, hence the need for continuous research to determine their sensitivity patterns.200212678447
asymptomatic bacteriuria in patients on continuous ambulatory peritoneal dialysis.chronic inflammatory state and chronic infections appear to be one of the most important risk factors of morbidity and mortality in patients (pts) on dialysis. the aim of our study was to determine the frequency of urinary tract infections (uti) in clinically asymptomatic patients on capd. the study included 43 (22f, 21m) clinically stable asymptomatic capd patients with diuresis ranged from 300 to 2500 ml per 24 h. clean catch urine specimen, was cultured and identified by standard technique. u ...200212898851
diagnosis, treatment and registration of urinary tract infections in geriatric patients.bacteriuria (> or = 10(5) cfu/ml) is a very common phenomenon in elderly people, occurring twice as frequently in women than in men. there are symptomatic and asymptomatic types of bacteriuria. risk factors include: a decrease in the estrogen level in women after the menopause, catheterisation, urinary bladder dysfunction, hypertrophy of the prostate gland, diabetes, neurological illnesses. the diagnosis of bacteriuria is based on quantitative urine culture (positive result--> or = 10(5) cfu/ml ...200212898972
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