Publications

in vitro activities of amphotericin b in combination with four antifungal agents and rifampin against aspergillus spp.	strains of aspergillus fumigatus, aspergillus flavus, and aspergillus niger were tested for in vitro susceptibility with a microtiter plate system in buffered yeast-nitrogen base and in buffered minimal essential medium. isolates were tested against amphotericin b, flucytosine, rifampin, ketoconazole, ici 153,066, and bay n 7133 and against combinations of amphotericin b with each of the other five drugs. combinations of amphotericin b and rifampin were the most active against all three species ...	1984	6329088
antimycotic activity of bay n 7133 in animal experiments.	the triazole derivative bay n 7133 has been tested for its antimycotic efficacy on oral administration in vivo and compared with ketoconazole in mice infected with candida albicans, aspergillus fumigatus and cryptococcus neoformans and in guinea pig trichophytosis. on starting administration at the same time as infection and using daily doses between 25 and 100 mg/kg, the agent protected the mice in all experimental models, even mouse aspergillosis for which ketoconazole was not adequately effec ...	1984	6330018
antimycotic azoles. 7. synthesis and antifungal properties of a series of novel triazol-3-ones.	a series of novel triazol-3-ones have been synthesized, and their in vitro and in vivo antifungal properties are reported. compound 68 (itraconazole), which displays a pronounced oral activity against vaginal candidosis in rats and against microsporosis in guinea pigs, has been selected for clinical evaluation.	1984	6330360
immunologic studies in bronchoalveolar fluid in a patient with allergic bronchopulmonary aspergillosis.	bronchoalveolar lavage was performed in a patient during an acute stage of allergic bronchopulmonary aspergillosis (abpa) and repeated 17 mo later during a remission stage and cessation of corticosteroid therapy. measurement of protein concentrations (albumin, igg, iga, and igm) by laser nephelometry and crossed immunoelectrophoresis indicates that transudation of proteins occurs from the circulation into the lung compartment during the acute stage of abpa. furthermore, comparing total ig concen ...	1984	6334111
effect of castanospermine on the structure and secretion of glycoprotein enzymes in aspergillus fumigatus.	aspergillus fumigatus secretes a number of glycosidases into the culture medium when the cells are grown in a mineral salts medium containing guar flour (a galactomannan) as the carbon source. at least some of these glycosidases have been reported to be glycoproteins having n-linked oligosaccharides. in this study, we examined the effect of the glycoprotein processing inhibitor, castanospermine, on the structures of the n-linked oligosaccharides and on the secretion of various glycosidases. cell ...	1984	6237100
extrinsic allergic alveolitis in the tobacco industry.	a total of 57 subjects who had been exposed to mould dust in the tobacco industry were studied. their working environment showed exposure to spores of different moulds, and 29 subjects (51%) showed antibodies against one or more of the microbes. fifteen (26%) had work related respiratory symptoms. eight (14%) showed slight radiographic pulmonary fibrosis. spirometry showed a tendency toward restriction and obstruction, especially in small airways. diffusion capacity was decreased in 18% of the w ...	1984	6318802
killing of aspergillus fumigatus spores and candida albicans yeast phase by the iron-hydrogen peroxide-iodide cytotoxic system: comparison with the myeloperoxidase-hydrogen peroxide-halide system.	a new fungicidal system composed of ferrous ion, h2o2, and iodide is described and compared with the myeloperoxidase-hydrogen peroxide-halide system. both systems had similar activity against aspergillus fumigatus spores and the candida albicans yeast phase, but only the ferrous ion-hydrogen peroxide-iodide system was inhibited by hydroxyl radical scavengers.	1984	6321349
in vitro activities of two new antifungal azoles.	the antifungal activities of equimolar quantities of three azole compounds, bay n 7133 [1-(4-chlorophenoxy)-3,3-dimethyl-2-(1,2,4-triazole-1-yl)methylbutan-2-o1], bay 1 19139 [1-(4-chlorophenoxy)-1-(1-imidazolyl)-3,3-dimethyl-2-butanol hydrochloride], and ketoconazole, were compared by testing the susceptibility in vitro of 10 clinical isolates each of candida albicans, candida parapsilosis, torulopsis glabrata, cryptococcus neoformans, aspergillus fumigatus, aspergillus niger, aspergillus flavu ...	1984	6721465
specific susceptibility to mucormycosis in murine diabetes and bronchoalveolar macrophage defense against rhizopus.	to assess the influence of diabetes mellitus in predisposing to pulmonary mucormycosis, a murine model of streptozotocin-induced diabetes was used. intranasal inoculation of rhizopus oryzae into diabetic mice resulted in mucormycotic infection with histopathology resembling pulmonary mucormycosis observed in humans. there was no mortality nor infection in inoculated normal mice. diabetic mice had fatal infections caused by r. oryzae but significantly reduced mortality following inoculation with ...	1984	6736246
primary cutaneous aspergillosis in six leukemic children.	we report a cluster of primary cutaneous aspergillosis in six children with hematologic malignancy. when first seen, they had hemorrhagic bullae caused by aspergillus flavus, aspergillus fumigatus, and aspergillus niger at the sites of insertion of intravenous cannulas or where arm boards had been taped to the extremities. rapid diagnosis of cutaneous aspergillosis was made by direct examination of the blister roof with potassium hydroxide before it progressed to a necrotic ulcer. intravenous am ...	1985	3855880
treatment of aspergillus fumigatus keratitis in rabbits with oral and topical ketoconazole.	aspergillus fumigatus keratitis was established in rabbits by intrastromal injection of a suspension containing 5,000 spores. after a 48-hour incubation period, the animals were treated with oral and topical ketoconazole alone and in combination with topical natamycin for five days. colony counts per cornea were obtained at the conclusion of the treatment period. based on these colony counts, neither oral nor topical ketoconazole was effective despite moderate in vitro sensitivity of the fungus ...	1985	3872596
surgery and granulocyte transfusions for life-threatening infections in chronic granulomatous disease.	we report two patients with chronic granulomatous disease (cgd) and life-threatening infections: a 10 10/12-year-old boy had aspergillus fumigatus spondylitis with destruction of the 11th vertebral body and paravertebral abscess formation, and an 8 5/12-year-old boy had multiple staphylococcus aureus hepatic abscesses with subphrenic abscess formation. both patients failed to respond to intense antimicrobial therapy but showed a remarkable recovery following surgical drainage combined with granu ...	1985	3878346
allergic bronchopulmonary aspergillosis.	allergic bronchopulmonary aspergillosis (abpa) occurs as a complication of asthma. it presents as an infiltrative pulmonary disorder with respiratory and systemic symptoms, eosinophilia, elevated total serum ige, and skin sensitizing, as well as precipitating antibodies to aspergillus fumigatus. sputum cultures are not always positive for the organism. if unchecked, the disease may lead to bronchiectasis and ultimately pulmonary fibrosis. therapy consists mainly of corticosteroids.	1985	3880535
limited reliability of the "negative" two-dimensional echocardiogram in the evaluation of infectious vegetative endocarditis: diagnostic and surgical implications.	three patients with vegetative endocarditis involving a native valve, a mechanical prosthesis and a bioprosthetic valve respectively are presented. each underwent emergency surgical exploration and prosthetic valve replacement based on clinical evidence of vegetative endocarditis after an initial delay following a report of a negative two-dimensional echocardiographic study. these cases emphasize the fact that within the clinical setting in which vegetative endocarditis is strongly suspected a n ...	1985	3881454
aspergilloma of the renal pelvis in a leukemic child.		1985	3881740
acute rejection and massive cyclosporine requirements in heart transplant recipients treated with rifampin.		1985	3883596
autoradiographic method to screen for soil microorganisms which accumulate zinc.	an autoradiographic method was developed to screen for and isolate soil microorganisms which accumulate zinc (zn). diluted soil samples (rubicon fine sand, entic haplorthods [ph 5.9]) were plated on soil extract-glucose agar containing radioactive 65zn. after 7 days of incubation, individual colonies which accumulated sufficient 65zn could be detected by autoradiography. these colonies were isolated and confirmed as zn accumulators in pure culture by using the autoradiographic plate technique. m ...	1985	3883897
the evaluation of a quantitative enzyme-linked immunosorbent assay (elisa) for anti-aspergillus fumigatus igg.	a standard enzyme-linked immunosorbent assay method for anti-aspergillus fumigatus igg in human serum was modified to produce a quantitative assay. the resulting assay was reproducible and capable of separating individual precipitin line groups and provided a means of monitoring the variation in antibody levels over long periods in patients with pulmonary aspergillosis.	1985	3884714
disseminated aspergillosis and bleeding colonic ulcers in renal transplant patient.		1985	3884810
contribution to the study of aspergillus fumigatus fresenius.	50 strains of aspergillus fumigatus were studied for enzymatic and physiologic profile. proteolytic and lipolytic activity were lacking. various ribonucleases and sacchrolytic enzymes were found.	1985	3885041
cardiac aspergillosis in acquired immune deficiency syndrome.		1985	3885709
chemotherapy of aspergillus fumigatus keratitis: an experimental study.	an experimental keratitis study of aspergillus fumigatus was performed in 130 rabbits divided into 12 groups of ten animals each. three antifungal drugs (myconazole, amphotericin b and pimaricin) were tested using two procedures (topical drops and subconjunctival injections) and two different concentrations (500 and 10 000 times the mic). in each case, the drugs were applied every 3 h starting 14 h after inoculation. miconazole was useful at 10 mg/ml concentration by topical drops and subconjunc ...	1985	3887174
bovine mycotic abortion in denmark.	a survey is given of the occurrence of mycotic infections associated with abortion in danish cattle. during a period of six years a total of 748 samples of placenta material were examined. mycotic abortion was demonstrated in 101 cases (14%). the case rate was significantly higher (21%) during the winter months from december through february than during the rest of the year (10%). abortions occurred as from the 135th day of pregnancy, with maximum in the eighth month, where 45% of all cases were ...	1985	3887326
intracranial granuloma caused by aspergillus fumigatus.	a case is reported of intracranial granuloma caused by aspergillus fumigatus involving the anterior cranial fossa and the frontal lobe. in this case, clinical symptoms developed about 5 years before the diagnosis was made. the final diagnosis was made by a craniotomy. the patient was treated with an extensive excision and chemotherapy, but finally he failed to respond to these treatments. we compile a summary of reported cases with a tabulation of pertinent information and discuss the pathogenes ...	1985	3887626
galactofuranosyl groups are immunodominant in aspergillus fumigatus galactomannan.	aspergillus fumigatus galactomannan was prepared and its structure partially characterized. galactofuranosyl groups were immunodominant when this polysaccharide antigen was reacted with antibody raised in rabbits.	1985	3889593
surgical management of symptomatic pulmonary aspergilloma.	pulmonary aspergilloma is a potentially life-threatening disease resulting from the colonization of lung cavities by the ubiquitous fungus aspergillus fumigatus. complex aspergilloma, characterized by thick-walled cavities with surrounding parenchymal inflammation, is a risk factor for increased morbidity and mortality. fifteen patients with symptomatic aspergilloma underwent major thoracic procedures at north carolina memorial hospital between january 1, 1972, and december 31, 1983. twelve of t ...	1985	3890782
[diagnosis of allergic bronchopulmonary aspergillosis: report of 3 cases].		1985	3891259
serum antibodies and their role in antibody-dependent cell-mediated cytotoxicity in aspergillosis.	a total of 22 sera from patients with aspergilloma and allergic bronchopulmonary aspergillosis (abpa) were examined concomitantly for specific antibody against aspergillus fumigatus antigen and for their activity in antibody-dependent cell-mediated cytotoxicity (adcc) against aspergillus antigen-coated target cells. these sera demonstrated significant precipitin bands in agar gel double diffusion test (78% of abpa and 75% of aspergilloma sera), while in indirect immunofluorescence studies all se ...	1985	3891592
antibody determination against aspergillus fumigatus by means of the enzyme-linked immunosorbent assay. i. liberation of polystyrene binding components during cultivation.	the liberation of polystyrene binding antigenic components (pbc) of aspergillus fumigatus involved in elisa igg measurements was studied during cultivation in the culture filtrates. pbc were rapidly liberated into the culture medium during the logarithmic phase of growth (phase i), were in part degraded or reconsumed during the stationary phase (phase ii) and again secreted (other pbc) during the lytic phase (phase iii). elisa igg titers and numbers of precipitating components are not always rel ...	1985	3891635
studies on toxin of aspergillus fumigatus. xxii. fashion of binding of asp-hemolysin to human erythrocytes and asp-hemolysin-binding proteins of erythrocyte membranes.	the fashion of binding of asp-hemolysin to human erythrocytes and the isolation of asp-hemolysin-binding proteins from erythrocyte membranes were investigated by the immunocytochemical technique and affinity chromatography. asp-hemolysin bound best at a ph range from 5 to 7. the erythrocytes treated with asp-hemolysin showed diffuse, ring-like or cap-like staining by the peroxidase-labeled antibody method under the light microscope. the distribution of asp-hemolysin on the erythrocyte surface wa ...	1985	3892239
the many faces of pulmonary aspergillosis.	the recognition of aspergillus in all its clinical disguises remains a challenge to clinicians in all fields of medicine. fortunately, aspergillus has low pathogenicity for humans and requires very heavy inoculum of spores (aspergillary pneumonitis) in order to infect those whose pulmonary defense mechanisms, inherent structure, and physiology are intact. patients with problems from aspergillus may be seen in either inpatient or outpatient clinical practice. the patient with fibrotic or cavitary ...	1985	3892565
frequency of aspergillus fumigatus isolates and antibodies to aspergillus antigens in cystic fibrosis.	the frequency of aspergillus fumigatus isolates from sputum was assessed prospectively during a 22-month period in 156 patients with cystic fibrosis (cf) from one center, and findings were compared to a cross-sectional evaluation of specific igg and iga antibodies, occurrence of chronic pseudomonas aeruginosa infection, and pulmonary function. the prevalence rate for the 22-month period was 40%. positive a. fumigatus cultures appeared to be independent of the presence or duration of chronic bron ...	1985	3893030
embolotherapy of a mycotic pseudoaneurysm of the internal mammary artery in chronic granulomatous disease.	we report a case demonstrating an unusual and previously unpublished complication of chronic granulomatous disease (cgd), that is massive hemorrhage from a mycotic pseudoaneurysm of the left internal mammary due to invasive aspergillus fumigatus of the anterior chest wall. this brief report further illustrates the angiographic diagnosis and embolotherapeutic control of a mycotic pseudoaneurysm of the internal mammary artery.	1985	3893696
rapid immunoelectrophoretic assay for detection of serum antibodies to aspergillus fumigatus catalase in patients with pulmonary aspergillosis.	a rapid immunoelectrophoretic assay was developed to detect antibodies to aspergillus fumigatus catalase. the method's diagnostic sensitivity for pulmonary aspergillosis was 88% (72-97%, 95% confidence limits) in 33 patients presenting with either aspergilloma or aspergillus lung infiltrate. the diagnostic specificity was 94% (90-97%) as judged from 191 patients with other infiltrative lung diseases, including infections and neoplasia. none of the 185 healthy subjects had catalase antibodies. th ...	1985	3894017
angioinvasive pulmonary aspergillosis: presentation as massive pulmonary saddle embolism in an immunocompromised patient.	a 33 year old woman with chronic myelogenous leukemia presented with clinical symptoms and hemodynamic signs suggestive of pulmonary embolism. initial angiographic studies supported the diagnosis of a massive saddle pulmonary embolus, and an inferior vena cava filter was inserted. however, subsequent autopsy revealed unsuspected angioinvasive pulmonary aspergillosis with secondary in situ thrombosis. the clinical features and diagnostic considerations in immunocompromised patients presenting wit ...	1985	3894476
[a case of aspergillosis of the ear].		1985	3895133
mycotic pseudoaneurysm of an aortic bypass graft and contiguous vertebral osteomyelitis due to aspergillus fumigatus.	fungal osteomyelitis is rare in patients who are not immunocompromised. this report documents that aspergillus vertebral osteomyelitis may, however, be associated with infection of an adjacent prosthetic vascular graft in the absence of overt immunosuppression. a 73-year-old man is described with a mycotic pseudoaneurysm of a dacron aortic bypass graft and contiguous vertebral osteomyelitis due to aspergillus fumigatus. the patient was successfully treated with resection of the infected graft an ...	1985	3895911
treatment of aspergillus fumigatus keratitis in rabbits with oral and topical ketoconazole.		1985	3895970
dual mycotic infection in a chicken.	a double mycotic infection, caused by aspergillus fumigatus and a zygomycete-like organism, was histopathologically demonstrated in the lungs of a 12-week-old chicken. a. fumigatus was isolated, but the zygomycete was not.	1985	3896222
[fungal pneumonia].		1985	3897098
[nasosinus aspergillosis].		1985	3898267
the occurrence of and organisms concerned with bovine mycotic abortion in some counties of ireland.	bovine mycotic abortion in some counties of the irish provinces of leinster and ulster was estimated at 1-2% of all abortions for which a causal agent was identified in the years from 1969 to 1978. the organism most commonly isolated from diseased specimens was aspergillus fumigatus, followed by absidia corymbifera. there was no relationship between the incidence of the disease in any year and the rainfall or number of raindays during the previous summer. the data obtained are discussed in relat ...	1985	3898556
aspergillus fumigatus thrombi causing total occlusion of both coronary arterial ostia, all four major epicardial coronary arteries and coronary sinus and associated with purulent pericarditis.		1985	3898801
detection of class-specific antibodies against aspergillus fumigatus antigens in various pulmonary diseases.	using an enzyme-linked immunosorbent assay (elisa) and a radioimmunoassay (rast), the specific iga, igg, igm and ige antibody levels against aspergillus fumigatus antigens were determined in sera from patients with either chronic bronchitis or non-allergic bronchial asthma, allergic asthma, allergic bronchopulmonary aspergillosis or aspergilloma. these levels were compared with those in serum samples from healthy controls. the results showed statistical differences in specific antibody levels be ...	1985	3898993
[enzyme-linked immunosorbent assay of serum igg antibodies to aspergillus fumigatus antigen using mouse monoclonal antibody to human igg].		1985	3899058
igg enzyme linked immunosorbent assay for diagnosis of invasive aspergillosis: retrospective study over 15 years of transplant recipients.	four commercially available aspergillus fumigatus antigens were compared in an igg enzyme linked immunosorbent assay by testing multiple sera from 19 patients who had received renal and hepatic allografts and who had histologically confirmed invasive aspergillosis. raised antibody levels were found in 16 of the 19 patients. more antibody responses were detected by culture filtrate antigens, but somatic antigens often detected antibodies sooner. when antibodies to aspergillus antigens were presen ...	1985	3900143
the diagnosis of invasive aspergillosis by an enzyme-linked immunosorbent assay for circulating antigen.	an inhibition enzyme-linked immunosorbent assay (elisa) capable of detecting 10 ng of aspergillus carbohydrate antigen/ml of serum was developed. when retrospectively applied to the sera of 19 patients with invasive aspergillosis, the elisa detected antigen in 11 patients. none of 14 healthy controls or 28 patients with a variety of other infections were positive for circulating antigen. a rabbit model of invasive aspergillosis was also developed. daily fungal cultures of blood were negative in ...	1985	3900240
[detection of aspergillosis among patients with lung diseases].		1985	3900990
methods for reducing particle concentrations of aspergillus fumigatus conidia and mouldy hay dust.	the effectiveness of commercially available domestic air purifiers to reduce airborne aspergillus fumigatus conidia and mouldy hay dust was investigated. it was found that the rate of particle clearance is a function of the volume of air passing through the purifiers but that the low throughflow of air makes their use of little value in clearing particles from a normal sized room. vacuum cleaners were more effective than air purifiers because of their higher air throughput, so too were high volu ...	1985	3901328
application of elisa-inhibition to aspergillus antigen standardization for immunodiagnosis.	an inhibition elisa method was used to test culture filtrate antigens of aspergillus fumigatus for the avidity of antibodies to them. four different culture media were used to check the ability of this assay to distinguish between similar antigenic preparations. differences between antigens exhibiting similar crossed immunoelectrophoresis patterns were observed by inhibition elisa.	1985	3901333
paraplegia due to aspergillosis. successful conservative treatment of two cases.	aspergillus infection of the spine is rare; for it to lead to paraplegia is still more rare. when this does occur it is usually treated by decompression and antifungal agents, but the results have usually been poor. we report two cases of successful conservative treatment of aspergillus paraplegia in patients with chronic granulomatous disease.	1985	3902849
[detection of antibodies to aspergillus fumigatus using enzyme immunoassay and counterimmunoelectrophoresis].		1985	3904255
immunoblot analysis of serological responses in invasive aspergillosis.	the serological response to aspergillus fumigatus was investigated in patients with pulmonary aspergillosis using the immunoblot technique. antibody was detected against nine components of the fungus, ranging in molecular weight from 88 000 to 33 000 daltons. antibody to a 40 000 dalton component was present in most patients (13 of 16). in surviving patients it was high (two with aspergilloma) or low but persistent (two with invasive aspergillosis). it was absent or fell in the 12 patients who d ...	1985	3905872
comparative pharmacokinetics of antifungal drugs in domestic turkeys, red-tailed hawks, broad-winged hawks, and great-horned owls.	the present research was to test in vitro activity of thiabendazole, 5-fluorocytosine, and amphotericin b against 11 isolates of aspergillus fumigatus from avian species. additionally, the plasma concentrations of these drugs were determined in four avian species given a range of dosages by oral, intravenous, and intratracheal routes. thiabendazole inhibited most isolates in vitro at concentrations between 25 and 50 micrograms/ml; however, there were no detectable inhibitory concentrations in th ...	1985	3907612
allergic bronchopulmonary aspergillosis and sensitization to aspergillus fumigatus in chronic bronchiectasis in adults.	fifty adult subjects for whom a diagnosis of idiopathic bronchiectasis (excluding those secondary to tuberculosis or hypogammaglobulinaemia) had been confirmed previously were investigated by: questionnaire; blood eosinophil count; sputum culture for aspergillus fumigatus and eosinophil count; chest radiography; skin-prick tests with several aeroallergens and four preparations of a. fumigatus, including a reference extract; measurement of specific ige antibodies; precipitin testing and self-cros ...	1985	3907888
[yield of the thoracoscopic biopsy in experimental pulmonary infections in the immunosuppressed rabbit].	using thoracoscopy lung biopsy we investigated the bacteriological diagnostic yield in immunodepressed and/or infected nz rabbits. 84 rabbits were used: 18 controls, 30 immunodepressed rabbits and 36 rabbits immunodepressed and then infected with aspergillus fumigatus. candida albicans or b.c.g. the thoracoscopy technique was the one we designed for humans, the instruments were adapted to the size of animals. thoracoscopy biopsy results were compared to those of surgical biopsies made in the sam ...	1985	3909265
[farmer's lung with antibodies against thermoactinomyces vulgaris and aspergillus fumigatus. clinical course and treatment].	we present a case of farmer's lung with antibodies to thermoactinomyces vulgaris and aspergillus fumigatus. a 56-year-old male patient with an atopic family medical history came to our hospital complaining of cough, dyspnea, fever, asthenia and anorexia. his condition worsened after being exposed to cereal powder, becoming symptomatic after 20 minutes or on occasion after 6 hours. physical examination showed basal crepitant rales in lung auscultation. the radiograph of the thorax showed a bilate ...	1985	3909794
familial aggregation of igg antibody response to antigens associated with farmer's lung.	the levels of circulating igg antibodies to aspergillus umbrosus, aspergillus fumigatus, thermoactinomyces vulgaris, and micropolyspora faeni were determined by enzyme immunoassay in 197 subjects selected for a study of farmer's lung (fl). the material consisted of five study groups: 37 patients with clinically confirmed fl, 31 spouses of the patients, 44 immediate relatives of the patients, 35 immediate relatives of the patients' spouses, and 50 unrelated people who were spouses of the 79 peopl ...	1985	3910599
comparison of serial diagnostic tests in a patient with invasive aspergillosis.	studies on a liver transplant recipient with fatal disseminated aspergillosis are described. the concentration of igg antibodies to aspergillus rose sharply with the onset of fever and changes in the chest x-ray, reaching a peak on day 10. thereafter, antibody concentrations fell and were within the normal range by day 21, when aspergillus was first isolated from an endotracheal aspirate and one day before death. the fall in antibodies preceded a rise in circulating immune complexes but aspergil ...	1985	3910731
primary pulmonary aspergillosis. a case study.		1985	3911067
aspergillus fumigatus osteomyelitis of the thoracic spine treated by excision and interbody fusion.		1985	3911444
[remote results of the surgical treatment of patients with pulmonary aspergillosis].	on the basis of an analysis of long-term results of the operative treatment of 17 patients (from 1 to 10 years) the development of possible complications associated with the aspergillous infection was established: aspergillous mycetoma with a bronchial fistula (in 3 patients), chronic aspergillous bronchopneumonia (in 10 patients), polycystosis of the lungs (in 3 patients). a conclusion is made of a necessary continuous dispensary follow-up of the patients operated upon and their treatment by an ...	1985	3911542
cultivation of fungi in synthetic and semi-synthetic liquid medium. ii. immunochemical properties of the antigenic and allergenic extracts.	four allergologically important fungi, viz. aspergillus fumigatus, penicillium notatum, alternaria alternata, and cladosporium herbarum were cultured in a liquid synthetic medium, with or without addition of 0.1% yeast extract (ye). after 10 and 28 days of cultivation, immunochemical properties of the fungal extracts, characterised by precipitation pattern and igg- and ige-binding capacity, were studied. in pure synthetic medium no large differences were observed in number of precipitates, as me ...	1985	3911818
galactose-reacting agglutinins to sporothrix schenckii.		1985	3914494
[allergic bronchopulmonary aspergillosis--individual cases or a problem?].		1985	3914634
systemic mycosis in scottish red deer (cervus elaphus).	the pathological features of 10 cases of systemic infection of young red deer by fungi morphologically identified as phycomycetes are described. the most frequently affected organs were the kidneys, followed by liver and central nervous system. the fungi stimulated an acute pyogenic inflammatory reaction and a phagocytic giant cell response. vasculitis with thrombosis and spread of fungi to the surrounding tissue was a common finding. culture was attempted in 4 cases; one yielded absidia, 2 aspe ...	1985	3915751
[the toxin-forming capacity of natural isolates of aspergillus fumigatus].		1985	3917185
antimicrobial activities of n-chloramines and diazolidinyl urea.	a combination of mics of an n-chloramine, a simple chlorinated amino acid, and diazolidinyl urea gave synergistic activity against bacteria, but not fungi. the two compounds at a higher concentration, 0.1 and 0.3%, respectively, gave synergistic inhibition of fungi; kill times were 1 h for trichophyton tonsurans, 3 h for aspergillus niger and fusarium moniliforme, and 6 h for aspergillus fumigatus.	1985	3920962
antigens of aspergillus fumigatus. iii. comparative immunochemical analyses of clinically relevant aspergilli and related fungal taxa.	cell sap (cs) of the pathogenic fungus aspergillus fumigatus strain ag-507 was fractionated by sephadex g-200 column chromatography. a protein fraction designated cs3 was partially characterized by two dimensional electrophoresis (2-de) and analytical ultracentrifugation. cs3 consisted mainly of low molecular weight components (14 k-43 k) of the whole cs, and produced one peak in analytical ultracentrifugation with an sapp of 4.25. cs3 was demonstrated to be different from a previously character ...	1985	3921301
[mycoses of the orbit and the eye].		1985	3922126
pulmonary aspergillosis in apparently healthy young rabbits.	pulmonary aspergillosis was discovered in apparently healthy young rabbits. thirty-five rabbits of various ages raised in the same rabbitry were examined by histological, ultrastructural and microbiological methods. the pulmonary lesions were found only in rabbits younger than five weeks old. these lesions contained characteristically shaped hyphae surrounded by eosinophilic radiating projections--"asteroid bodies." the significance of the disease condition and the structure of the "asteroid bod ...	1985	3923689
the report of kaufman et al. on the relationship between agar gel double-diffusion and elisa results for aspergillus fumigatus igg antibody.		1985	3924983
[peracute disseminated course of fatal aspergillus fumigatus infection in liver failure and corticoid therapy. a case report on the epidemiology, pathogenesis and diagnosis of the systemic course of aspergillus infections].	cytostatic-treated persons and cases of severe hepatic failure under corticoid therapy are predisposed to disseminating aspergillus infections. constant exposure to aspergillus spores may result in a fatal aspergillus infection. the triad of hepatic failure, corticoid therapy and constant exposure to aspergillus spores is described in a 70-year-old female patient. a painless icterus was clinically diagnosed as non-a non-b hepatitis, with a protracted cholestatic course. she had been treated with ...	1985	3925220
[immunodiagnosis of aspergillus-induced bronchopulmonary diseases].	studies using the immunoblot technique were carried out to identify the components of aspergillus fumigatus against which the ige and igg antibodies of 22 patients with aspergillus-related lung diseases are directed. asthmatics exhibit ige antibodies against 2-6 of the fungus components and, surprisingly, an even more pronounced igg antibody reaction. it was determined that persons suffering from allergic bronchopulmonary aspergillosis show massive ige-and igg-mediated sensitization, generally a ...	1985	3925546
serum antibodies to aspergillus fumigatus in danish farmers.	182 danish farmers and 105 city-dwelling control subjects were investigated for serum igg antibodies to three purified aspergillus fumigatus antigen fractions and unfractionated culture filtrate by enzyme-linked immunosorbent assay (elisa). farmers had higher levels of antibodies to all four elisa antigens than non-farming controls. in farmers and controls high antibody activity was recorded with an antigen fraction of approximate molecular weight 470 000 daltons. antibody levels to this fractio ...	1985	3925571
a method to determine significant levels of immunoglobulin g to aspergillus fumigatus antigens in an elisa system and a comparison with counterimmunoelectrophoresis and double diffusion techniques.	a method is described which assesses results obtained from an elisa system for the determination of human serum levels of igg class antibodies to aspergillus fumigatus. the method is used to discriminate positive from negative samples, and significant antibody activity may be reported to the clinician, relative to a reference positive control serum monitored simultaneously under the same test conditions. antibody content is expressed as the absorbance of a certain dilution of serum. duplicate sa ...	1985	3926825
diagnosis of pulmonary infection caused by aspergillus: usefulness of respiratory cultures.	aspergillus infection is often difficult to diagnose, and the usefulness of methods of culture for diagnosis has been questioned. we reviewed all respiratory cultures that yielded any species of aspergillus and all surgical and autopsy materials suspected or diagnostic of infection with aspergillus over a two-year period. of 89 patients with one or more positive cultures of nonbiopsy respiratory tract specimens, eight had histologically proven disease (three had aspergillomas and five, invasive ...	1985	3928772
use of triton-x-100 for improving aspergillus fumigatus antigen(s) and antigenicity.		1985	3929082
antibody determination against aspergillus fumigatus by means of the enzyme-linked immunosorbent assay. ii. physico- and immunochemical properties of the polystyrene-binding components.	polystyrene-binding antigenic components (pbc) of aspergillus fumigatus involved in elisa igg antibody determinations and the corresponding numbers of precipitates found by double immunodiffusion (did) and/or crossed immunoelectrophoresis (cie) were studied using culture filtrate antigenic preparations fractionated by sephadex g-100 chromatography, concanavalin a (cona) affinity chromatography, isoelectric focusing (ief) and tca precipitation. it is shown that pbc, immunologically active in elis ...	1985	3930408
a method to determine significant levels of immunoglobulin g to aspergillus fumigatus antigens in an elisa system and a comparison with counterimmunoelectrophoresis and double diffusion techniques.	a method is described which assesses results obtained from an elisa system for the determination of human serum levels of igg class antibodies to aspergillus fumigatus. the method is used to discriminate positive from negative samples, and significant antibody activity may be reported to the clinician, relative to a reference positive control serum monitored simultaneously under the same test conditions. antibody content is expressed as the absorbance of a certain dilution of serum. duplicate sa ...	1985	3930574
therapeutic concentrations of glucocorticoids suppress the antimicrobial activity of human macrophages without impairing their responsiveness to gamma interferon.	by exposing human blood-derived macrophages and alveolar macrophages in vitro to dexamethasone, we showed in these studies that glucocorticoids markedly suppress the antimicrobial activity of macrophages but not macrophage activation by lymphokines. as little as 2.5 x 10(-8) mol/liter of dexamethasone prevented macrophages from inhibiting germination of aspergillus spores or from eliminating ingested bacteria such as listeria, nocardia, or salmonella. damage to macrophage function was inhibited ...	1985	3932471
the effect of phospholipids from listeria monocytogenes and aspergillus fumigatus on degradation of bacterial antigens by mouse peritoneal macrophages.	investigations were carried out on an influence of phospholipids from l. monocytogenes and a. fumigatus on the degradation of streptococcus faecalis cell walls by mouse peritoneal macrophages. it has been found, that macrophages from mice stimulated with phospholipids under studies degraded streptococcal cell walls labeled with dl-alanine-i-14c more efficiently than normal macrophages. activated macrophages degraded also more rapidly antigenic structures of bacterial cell walls reacting with spe ...	1985	3933450
[aspergilloma of the lung].		1985	3934455
variation of a 470 000 daltons antigen complex and catalase antigen in clinical isolates of aspergillus fumigatus.	antigens in ruptured mycelium of 18 aspergillus strains including 14 clinical isolates of a. fumigatus were studied by immunoelectrophoresis. one antigenic component of molecular weight 470 000 previously characterized by hydrophobic interaction chromatography and gel filtration and a second component with catalase activity were detected in all a. fumigatus isolates but in varying quantities. the 470 000 antigen complex cross-reacted with antigens in a. flavus and a. nidulans but not in a. niger ...	1985	3934773
aspergillosis of the nose and paranasal sinuses in neutropenic patients at an oncology center.	invasive aspergillosis of the nose and paranasal sinuses is one of the presentations of aspergillosis in granulocytopenic patients with neoplastic disorders. it is most prevalent among patients with leukemia and granulocytopenia and is associated with a high mortality rate. we report five cases of invasive aspergillosis of the nose and paranasal sinuses in profoundly neutropenic patients treated with broad spectrum antibiotics. both aspergillus fumigatus and aspergillus flavus were cultured and ...	1985	3935605
precipitating antibodies against micropolyspora faeni in equines in north-western india.	prevalence of serum precipitins against micropolyspora faeni, thermoactinomyces vulgaris and aspergillus fumigatus, employing the counterimmunoelectrophoresis (coie) and ouchterlony's double diffusion (dd) techniques, is reported in 162 of the equines stationed at two military installations in north-western india. m. faeni specific precipitins were demonstrable in 58 of 112 mules from site i in the mountainous region whereas the results were negative for all of the 50 horses examined from site i ...	1985	3937491
investigations on the sporicidal and fungicidal activity of disinfectants.	the sporicidal and fungicidal activity of disinfectants was studied in a suspension test. glutaraldehyde 4%, sodium-dichloroisocyanurate-dihydrate (2400 ppm active chlorine) and peracetic acid 0.25% demonstrated after 30 min of exposure at 20 degrees c in the presence of 4% horse serum a clear activity against spores of bacillus cereus. under the same conditions formaldehyde 4% and glutaraldehyde 2% were also found to be sporicidal, but only after a longer time of exposure. spores of bacillus an ...	1985	3938146
[characterization of exoantigens of aspergillus fumigatus. study of factors influencing their quality].	several methods are available for exoantigen preparation and standardization, but until now there is no agreement about which is the best of them. moreover an exhaustive analysis of the influence of several experimental conditions on the antigenic composition of exoantigens of aspergillus fumigatus is badly needed. in this paper the analysis of the influence of the following conditions on this antigenic composition is described (origin of strains, culture media composition, incubation time of my ...	1985	3938616
pleural fluid as nutrient substratum for aspergillus fumigatus and a. flavus. submerged growth in pleural fluid and extracellular proteolysis in pleural fluid agar. a preliminary report.	pleural fluid in liquid and agar form from a patient with adenocarcinoma of the lung was used as a nutrient medium for culturing aspergillus fumigatus and a, flavus. the extracellular proteolytic activity of both strains could be demonstrated after an incubation period of 5 d at 37 degrees c by the agar block method using pleural fluid agar (protein content ca. 0.1-0.5% and initial ph values of 7.0 and 5.0) and protein staining with naphthalene black. in the undiluted pleural fluid, after statio ...	1985	3938910
[production and standardization of paracoccidioides brasiliensis, histoplasma capsulatum and aspergillus fumigatus antigens to be used in immunodiagnosis. comparison between immunodiffusion and immunoelectrophoresis].	soluble antigens (ag) from paracoccidioides brasiliensis, histoplasma capsulatum and aspergillus fumigatus were prepared and standardized by double immunodiffusion (did) and immunoelectroosmophoresis (ieop). no difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positive with p. brasiliensis and a. fumigatus ag and 83.3% were positive with h. capsulatum ag. the specificity of the tests was verified testing 96 sera from patients with paracoccidioi ...	1985	3939250
[effect of the relative humidity of the air and of the attendant microflora on aflatoxin formation in food concentrates].		1985	3939832
allergic bronchopulmonary aspergillosis in indian children with bronchial asthma.	out of 243 children with bronchial asthma, 107 children (44%) were found to have perennial asthma. these children were studied to determine the incidence of allergic bronchopulmonary aspergillosis (abpa) in indian children. clinical and radiologic evaluations, skin testing, and precipitin studies with aspergillus fumigatus antigen, and total ige level estimations were done. fifteen percent of children had four or more of the criteria, and were considered to have abpa.	1985	3966689
immunomodulation of the antibody response to lipopolysaccharide in c3h/hej mice by complexing with heterologous ribosomes.	we show that formaldehyde fixation of salmonella typhimurium lipopolysaccharide (lps) to ribosomes purified from brucella abortus induced a primary immunoglobulin m (igm) response to lps in c3h/hej mice and upon revaccination resulted in elevated titers of igm and induction of igg antibody to the o antigens of lps, as measured by an enzyme-linked immunosorbent assay. a similar lps-aspergillus fumigatus ribosomal complex yielded igm and igg antibody to lps only after secondary stimulation. these ...	1985	3980087
standardization of a hyphal inoculum of aspergilli for amphotericin b susceptibility testing.	standardized, evenly dispersed hyphal suspensions served as the inoculum in a microtiter technique for amphotericin b antifungal susceptibility testing. preliminary testing with six strains of aspergillus fumigatus and a. flavus produced consistent and reproducible results at 30 degrees c over 24 h. the observed amphotericin b mics required for hyphae (0.3 to 0.6 microgram/ml) were comparable to mics required for conidia (0.16 to 0.6 microgram/ml). the results were evaluated and compared with pr ...	1985	3988896
the effect of ketoconazole on amphotericin b in a model of disseminated aspergillosis.	the potential of ketoconazole prophylaxis to antagonize the activity of amphotericin b against aspergilli was investigated in vitro and in neutropenic mice. exposure of aspergillus fumigatus (six strains) and of aspergillus flavus or aspergillus niger to ketoconazole resulted in a uniform increase of the minimal fungicidal activity of amphotericin b, from 0.15-0.63 mg/liter to greater than 2.5 mg/liter in a microwell assay. to test the relevance of this antagonism in vivo, we challenged neutrope ...	1985	3989324
complete amino acid sequence of the aspergillus cytotoxin mitogillin.	the complete amino acid sequence of the cytotoxin mitogillin has been determined by sequencing the intact chain and peptide fragments produced by cleavage at methionyl, arginyl, lysyl, and tryptophanyl residues and at one aspartic acid-proline bond. the protein consists of 149 amino acid residues with alanine at the nh2 terminus and histidine at the cooh terminus. the calculated mr of the native mitogillin was 16 867. the native molecule presents two disulfide bridges, one between cysteine resid ...	1985	3994994
the mode of action of restrictocin and mitogillin on eukaryotic ribosomes. inhibition of brain protein synthesis, cleavage and sequence of the ribosomal rna fragment.	the relationship between the process of rat brain protein synthesis inhibition by restrictocin and mitogillin and the induction of a specific cleavage in 28s rrna has been examined. restrictocin or mitogillin at a concentration of 6 nm inhibits protein synthesis to a level of 80%. the inhibition induced by 6 nm alpha-sarcin is of the same order, indicating that those molecules all catalytically inhibit protein synthesis. when restrictocin or mitogillin was reduced and alkylated, a 100-fold highe ...	1985	3996402
bronchoesophageal fistula caused by pulmonary aspergillosis.	a 29-year-old patient developed a bronchoesophageal fistula after expectorating a mycetoma caused by aspergillus fumigatus. after failure of medical therapy, a retrosternal coloplasty was performed. pulmonary aspergillosis can be the cause of a bronchoesophageal fistula.	1985	4018188
asthma and allergic aspergillosis in monozygotic twins.	monozygotic twins are reported; both had bronchial asthma with type i hypersensitivity to aspergillus fumigatus but only one had type iii hypersensitivity, together with pulmonary infiltrations compatible with the diagnosis of allergic bronchopulmonary aspergillosis. it is suggested that non-inherited factors other than the intensity of exposure to antigen may be important in determining the development of allergic bronchopulmonary aspergillosis.	1985	4040385
calcofluor and ink-potassium hydroxide preparations for identifying fungi.	calcofluor and ink-potassium hydroxide preparations identified fusarium solani, aspergillus fumigatus, and candida albicans, the three most common ocular fungal pathogens, in scrapings, biopsy specimens, and tissue sections of corneal mycotic infections in rabbits. these stains also identified fungal organisms in specimens from four human patients with keratomycoses. neither procedure requires more than a few minutes to perform or extensive training or experience to interpret. the specimen stain ...	1985	4061554