Publications

The CfMNPV LEF-3/P143 complex can complement DNA replication and budded virus production in an AcMNPV LEF-3/P143 double knockout bacmid.	Transient replication assays using Autographa californica nucleopolyhedrovirus and Choristoneura fumiferana nucleopolyhedrovirus genes suggested the interactions between P143, the viral helicase, and LEF-3, a single-strand DNA binding protein, may represent virus species specificity determinants. P143 and LEF-3 are essential for DNA replication in these assays and together with IE-1, the major immediate-early transcription factor, may be part of the viral replisome. In the current report, a lef- ...	2011	22049089
Identification of Autographa californica nucleopolyhedrovirus ac93 as a core gene and its requirement for intranuclear microvesicle formation and nuclear egress of nucleocapsids.	Autographa californica nucleopolyhedrovirus (AcMNPV) orf93 (ac93) is a highly conserved uncharacterized gene that is found in all of the sequenced baculovirus genomes except for Culex nigripalpus NPV. In this report, using bioinformatics analyses, ac93 and odv-e25 (ac94) were identified as baculovirus core genes and thus p33-ac93-odv-e25 represent a cluster of core genes. To investigate the role of ac93 in the baculovirus life cycle, an ac93 knockout AcMNPV bacmid was constructed via homologous ...	2011	21880748
modulation of innate immunity in chickens induced by in vivo administration of baculovirus.	baculoviruses stimulate cytokine production in mammalian cells. they induce a strong innate immune response in animals and have adjuvant properties. the purpose of this work was to study the in vivo effect of baculovirus on chicken innate immune response. spf chickens were inoculated intravenously with autographa californica nuclear polyhedrosis virus (bv). three hours later, chickens were bled, euthanized and their spleen, duodenum and cecal tonsils were excised in order to take samples for rna ...	2011	22142984
new insights into the induction of the heat shock proteins in baculovirus infected insect cells.	eight members of the hsp/hsc70 family were identified in spodoptera frugiperda sf9 cells infected with autographa californica multiple nucleopolyhedrovirus (acmnpv) by 2d electrophoresis followed by mass spectrometry (maldi/tof) and a mascot search. the family includes five hsp70s induced by acmnpv-infection and three constitutive cognate hsc70s that remained abundant in infected cells. confocal microscopy revealed dynamic changes in subcellular localization of hsp/hsc70s in the course of infect ...	2011	21982219
establishment and characterization of an embryonic cell line from gampsocleis gratiosa (orthoptera: tettigoniidae).	the first continuous cell line from the embryo of gampsocleis gratiosa (orthoptera: tettigoniidae), designated as riri-gg1, was established. this cell line was serially subcultured in modified grace medium. the cells were grown adherent to a culture flask and had spindle-like and polygonal shapes. the chromosome number ranged from 26 to 79 at the 50th passage, and 68% of cells had a diploid chromosome number. the growth rate was determined at the 53rd passage, and the population doubling time wa ...	2011	21424241
genetic variation and virulence of nucleopolyhedroviruses isolated worldwide from the heliothine pests helicoverpa armigera, helicoverpa zea, and heliothis virescens.	to assess the diversity and relationships of baculoviruses found in insects of the heliothine pest complex, a pcr-based method was used to classify 90 samples of nucleopolyhedrovirus (npv; baculoviridae: alphabaculovirus) obtained worldwide from larvae of heliothis virescens, helicoverpa zea, and helicoverpa armigera. partial nucleotide sequencing and phylogenetic analysis of three highly conserved genes (lef-8, lef-9, and polh) indicated that 67 of these samples contained isolates of the h. zea ...	2011	21439295
autographa californica multiple nucleopolyhedrovirus odv-e66 is an essential gene required for oral infectivity.	autographa californica multiple nucleopolyhedrovirus (acmnpv) odv-e66 is a core gene and encodes an occlusion-derived virus (odv)-specific envelope protein, odv-e66. the n-terminal 23 amino acid of the envelope protein odv-e66 are sufficient to direct native and fusion proteins to induced membrane microvesicles and the viral envelope during infection with acmnpv. in this study, an odv-e66-knockout bacmid can not express n-terminal hydrophobic domains was constructed via homologous recombination ...	2011	21440017
baculovirus vp80 protein and the f-actin cytoskeleton interact and connect the viral replication factory with the nuclear periphery.	recently, we showed that the autographa californica multicapsid nucleopolyhedrovirus (acmnpv) vp80 protein is essential for the formation of both virion types, budded virus (bv) and occlusion-derived virus (odv). deletion of the vp80 gene did not affect assembly of nucleocapsids. however, these nucleocapsids were not able to migrate from the virogenic stroma to the nuclear periphery. in the current paper, we constructed a baculovirus recombinant with enhanced-green fluorescent protein (egfp)-tag ...	2011	21450830
baculoviruses mediate efficient gene expression in a wide range of vertebrate cells.	baculovirus expression vector system (bevs) is well known as a feasible and safe technology to produce recombinant (re-)proteins in a eukaryotic milieu of insect cells. however, its proven power in gene delivery and gene therapy is still poorly recognized. the basis of bevs lies in large enveloped dna viruses derived from insects, the prototype virus being autographa californica multiple nucleopolyhedrovirus (acmnpv). infection of insect cell culture with a virus encoding a desired transgene und ...	2011	21590402
the autographa californica multiple nucleopolyhedrovirus lef-5 gene is required for productive infection.	to examine the role of the acmnpv lef-5 gene in the context of the infection cycle, we generated an acmnpv lef-5 knockout virus (vac(lef5ko)) and a complementing cell line that supports viral replication. we examined acmnpv dna replication, early and late gene expression, and production of infectious viral progeny in the absence of lef-5. while early gene expression and dna replication were not reduced by the lef-5 knockout, expression of a late reporter was disrupted and representative late tra ...	2011	21601232
kinetic characterization of the group ii helicoverpa armigera nucleopolyhedrovirus propagated in suspension cell cultures: implications for development of a biopesticides production process.	large-scale commercialization of baculovirus biopesticides for the control of insect pests requires a cell culture production process, and knowledge of the infection kinetics is a vital prerequisite for process optimization. well-characterized kinetic parameters have so far only been reported for the commercially established recombinant autographa californica nucleopolyhedrovirus (acmnpv), a group i npv. in this work, key infection kinetic parameters of the group ii npv helicoverpa armigera nucl ...	2011	21644255
active depletion of host cell inhibitor-of-apoptosis proteins triggers apoptosis upon baculovirus dna replication.	apoptosis is an important antivirus defense by virtue of its impact on virus multiplication and pathogenesis. to define molecular mechanisms by which viruses are detected and the apoptotic response is initiated, we examined the antiviral role of host inhibitor-of-apoptosis (iap) proteins in insect cells. we report here that the principal iaps, diap1 and sfiap, of the model insects drosophila melanogaster and spodoptera frugiperda, respectively, are rapidly depleted and thereby inactivated upon i ...	2011	21653668
establishment, growth kinetics, and susceptibility to acmnpv of heat tolerant lepidopteran cell lines.	lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several bombyx cells. they have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. in this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. adaptation of cell lines sf-9, bti-tn-5b1-4 (high5) and bti-tn-mg1 (mg1) to 33-¦c and 35-¦c was carried out by shift ...	2011	21667340
baculovirus as vectors for human cells and applications in organ transplantation.	the baculovirus autographa californica multiple nucleopolyhedrovirus (acmnpv) is able to transduce a wide range of mammalian cells and shows preferential uptake in some, particularly liver and kidney cells. this suggests that the virus may be useful for delivery of protective genes for ameliorating the effects of ischaemia reperfusion injury (iri) in solid organs during transplantation procedures. in this chapter we discuss the advantages of the baculovirus over other virus vectors for gene deli ...	2011	21784231
secretory expression of all 16 subtypes of the hemagglutinin 1 protein of influenza a virus in insect cells.	influenza viruses are known for their ability to change their antigenic structure and create new viral strains. hemagglutinin (ha), for which 16 subtypes have been identified, is a major antigenic determinant essential for the pathogenesis of influenza a viruses. to predict and monitor future epidemics, it is critical to produce various ha subtype antigens conveniently and rapidly. a simple, effective, and economic method to generate subunit ha1 of all 16 ha subtypes as recombinant proteins (rha ...	2011	21827791
baculovirus envelope protein odv-e66 is a novel chondroitinase with distinct substrate specificity.	chondroitin sulfate is a linear polysaccharide of alternating d-glucuronic acid and n-acetyl-d-galactosamine residues with sulfate groups at various positions of the sugars. it interacts with and regulates cytokine and growth factor signal transduction, thus influencing development, organ morphogenesis, inflammation, and infection. we found chondroitinase activity in medium conditioned by baculovirus-infected insect cells and identified a novel chondroitinase. sequence analysis revealed that the ...	2011	21715327
[the search of microrna genes encoded in antiparallel chains of autographa californica nuclear polyhedrosis virus genome regions, including most late and complementary genes].	it was shown using bioinformatic approach by analysis of alternative transcriptes secondary structure, that a. californica nuclear polyhedrosis virus gene ph encoded two mature mirnas and three potential mirnas. gene orf1629 complementary to gene ph did not encode mirnas and pre-mirna-cs. gene p10 encodes mature and potential mirna. gene p74 located on complementary chain encodes three mature mirnas.	2011	22145408
Susceptibility to AcMNPV and expression of recombinant proteins by a novel cell clone derived from a Trichoplusia ni QAU-BTI-Tn9-4s cell line.	It is well known that Tn5B1-4 (commercially known as the High Five) cell line is highly susceptible to baculovirus and provides superior production of recombinant proteins when compared to other insect cell lines. But the characteristics of the cell line do not always remain stable and may change upon continuous passage. Recently an alphanodavirus, named Tn5 Cell Line Virus (or TNCL Virus), was identified in High Five cells in particular. Therefore, we established a new cell line, QB-Tn9-4s, fro ...	2011	21979569
Baculovirus GP64-mediated entry into mammalian cells.	The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) serves as an efficient viral vector not only for abundant gene expression in insect cells but also for gene delivery into mammalian cells. Lentivirus vectors pseudotyped with the baculovirus envelope glycoprotein GP64 have been shown to acquire more potent gene transduction than those with vesicular stomatitis virus (VSV) envelope glycoprotein G. However, there are conflicting hypotheses about the molecular mechanisms ...	2011	22190715
Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy.	Autographa californica multiple nucleopolyhedrovirus (AcMNPV) replicates in the nucleus of insect cells to produce nucleocapsids, which are transported from the nucleus to the plasma membrane for budding through GP64-enriched areas to form budded viruses. However, little is known about the anterograde trafficking of baculovirus nucleocapsids in insect cells. Preliminary confocal scanning laser microscopy studies showed that enhanced green fluorescent protein (EGFP)-tagged nucleocapsids and capsi ...	2012	22072745
development of a novel baculovirus titration method using the enzyme-linked immunosorbent spot (elispot) assay.	the baculovirus expression vector system (bevs) is one of the most powerful methods for production of recombinant proteins for research or commercial purposes. titration of viable virus in insect cell culture is often required when bevs is used for basic research or bioprocessing. an enzyme-linked immunosorbent spot (elispot) assay using monoclonal antibodies against the major capsid protein vp39 of both autographa californica nuclear polyhedrosis virus (acmnpv) and bombyx mori nuclear polyhedro ...	2012	23274754
cellular vps4 is required for efficient entry and egress of budded virions of autographa californica multiple nucleopolyhedrovirus.	membrane budding is essential for the egress of many enveloped viruses, and this process shares similarities with the biogenesis of multivesicular bodies (mvbs). in eukaryotic cells, the budding of intraluminal vesicles (ivls) is mediated by the endosomal sorting complex required for transport (escrt) machinery and some viruses require escrt machinery components or functions to bud from host cells. baculoviruses, such as autographa californica multiple nucleopolyhedrovirus (acmnpv), enter host c ...	2012	22072775
stable protein expression in mammalian cells using baculoviruses.	the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) has been widely used in biotechnology for protein expression in insect cells. baculoviruses use arthropods as their natural hosts and are unable to replicate in mammalian cells. however, acmnpv is able to enter many mammalian cell types and can be used for transgene expression if engineered to contain suitable expression cassettes. in this chapter, we describe the construction and application of a recombinant bac ...	2012	21987248
Exploring sub-lethal effects of exposure to a nucleopolyhedrovirus in the speckled wood (Pararge aegeria) butterfly.	This study investigated the sub-lethal effects of larval exposure to baculovirus on host life history and wing morphological traits using a model system, the speckled wood butterfly Pararge aegeria (L.) and the virus Autographa californica nucleopolyhedrovirus. Males and females showed similar responses to the viral infection. Infection significantly reduced larval growth rate, whilst an increase in development time allowed the critical mass for pupation to be attained. There was no direct effec ...	2012	22064244