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specific poly-phenolic compounds in cell culture of vitis vinifera l. cv. gamay fréaux.cell cultures established from plants represent an attractive alternative to whole plants for effective production of bioactive secondary metabolites. cell culture from vitis vinifera l. cv. gamay fréaux accumulated high amounts of hydroxycinnamic acid derivatives and anthocyanins. two new compounds were identified: 3-o-glucosylresveratrol, a stilbene derivative, abundant in cell suspension culture, and a hydroxyphenol, 4-(3,5-dihydroxyphenyl)-phenol, abundant in callus culture. the major anthoc ...201121061087
seasonal changes of whole root system conductance by a drought-tolerant grape root system.the role of root systems in drought tolerance is a subject of very limited information compared with above-ground responses. adjustments to the ability of roots to supply water relative to shoot transpiration demand is proposed as a major means for woody perennial plants to tolerate drought, and is often expressed as changes in the ratios of leaf to root area (a(l):a(r)). seasonal root proliferation in a directed manner could increase the water supply function of roots independent of total root ...201120851906
water deficit increases stilbene metabolism in cabernet sauvignon berries.the impact of water deficit on stilbene biosynthesis in wine grape (vitis vinifera) berries was investigated. water deficit increased the accumulation of trans-piceid (the glycosylated form of resveratrol) by 5-fold in cabernet sauvignon berries but not in chardonnay. similarly, water deficit significantly increased the transcript abundance of genes involved in the biosynthesis of stilbene precursors in cabernet sauvignon. increased expression of stilbene synthase, but not that of resveratrol-o- ...201121128664
characterization of expression dynamics of wox homeodomain transcription factors during somatic embryogenesis in vitis vinifera.different cultivars of vitis vinifera vary in their potential to form embryogenic tissues. the wuschel (wus)-related homeobox (wox) genes have been shown to play an important role in coordinating the gene transcription involved in the early phases of embryogenesis. the expression dynamics of 12 vvwox genes present in the v. vinifera genome in embryogenic and other tissues of 'chardonnay' were analysed. in order to understand the influence of wox genes on the somatic embryogenic process, their ex ...201121127025
environmental stress enhances biosynthesis of flavor precursors, s-3-(hexan-1-ol)-glutathione and s-3-(hexan-1-ol)-l-cysteine, in grapevine through glutathione s-transferase activation.the biosynthesis of s-(3-hexan-1-ol)-glutathione (3mh-s-glut) and s-(3-hexan-l-ol)-l-cysteine (3mh-s-cys), which act as flavour precursors in wines, in vitis vinifera grapes exposed to various environmental stress conditions is reported here. ultraviolet (uv-c) irradiation, water deficit, and biological stimulation up-regulated 3mh-s-glut and 3mh-s-cys biosynthesis in grape leaves. 3mh-s-glut and 3mh-s-cys contents in grape berries were increased by cold shock, heat shock, uv-c irradiation, and ...201121115666
epigenetic repressor-like genes are differentially regulated during grapevine (vitis vinifera l.) development.grapevine sexual reproduction involves a seasonal separation between inflorescence primordia (flowering induction) and flower development. we hypothesized that a repression mechanism implicating epigenetic changes could play a role in the seasonal separation of these two developmental processes in grapevine. therefore, the expression of five grapevine genes with homology to the arabidopsis epigenetic repressor genes fertilization independent endosperm (fie), embryonic flower 2 (emf2), curly leaf ...201121681473
transcriptome analysis of sarracenia, an insectivorous plant.sarracenia species (pitcher plants) are carnivorous plants which obtain a portion of their nutrients from insects captured in the pitchers. to investigate these plants, we sequenced the transcriptome of two species, sarracenia psittacina and sarracenia purpurea, using roche 454 pyrosequencing technology. we obtained 46 275 and 36 681 contigs by de novo assembly methods for s. psittacina and s. purpurea, respectively, and further identified 16 163 orthologous contigs between them. estimation of s ...201121676972
extensin network formation in vitis vinifera callus cells is an essential and causal event in rapid and h(2)o(2)-induced reduction in primary cell wall hydration.extensin deposition is considered important for the correct assembly and biophysical properties of primary cell walls, with consequences to plant resistance to pathogens, tissue morphology, cell adhesion and extension growth. however, evidence for a direct and causal role for the extensin network formation in changes to cell wall properties has been lacking.201121672244
the role of light in the regulation of ascorbate metabolism during berry development in the cultivated grapevine vitis vinifera l.the accumulation of l-ascorbate (asc) in fruits is influenced by environmental factors including light quantity. fruit exposure to ambient light is often reduced by the surrounding leaf canopy, and can be altered by cultivation practices. the influence of reduced sunlight exposure on the accumulation of asc and its catabolites was investigated in field-grown berries of the cultivated grapevine vitis vinifera l. cv. shiraz.201121656772
accumulation and distribution pattern of macro- and microelements and trace elements in vitis vinifera l. cv. chardonnay berries.this paper describes the accumulation pattern of 42 mineral elements in vitis vinifera l. berries during development and ripening and their distribution in berry skin, seeds, and flesh around harvest time. grape berries were sampled in two different vineyards with alkaline soil and analyzed using a icp-ms. although elemental amounts were significantly different in the grapes from the two vineyards, the accumulation pattern and percentage distribution in different parts of the berries were genera ...201121639148
restructuring of endophytic bacterial communities in grapevine yellows-diseased and recovered vitis vinifera l. plants.length heterogeneity-pcr assays, combined with statistical analyses, highlighted that the endophytic bacterial community associated with healthy grapevines was characterized by a greater diversity than that present in diseased and recovered plants. the findings suggest that phytoplasmas can restructure the bacterial community by selecting endophytic strains that could elicit a plant defense response.201121622794
anthocyanin composition and extractability in berry skin and wine of vitis vinifera l. cv. aglianico.the present article reports the anthocyanin content in the berry skin and wine of the italian red grape cultivar aglianico (clone vcr11 grafted onto 1103 paulsen), one of the most ancient vines and famous for its deep-red colour. anthocyanins were extracted from frozen berry skin in an acidified methanol solution. the extraction mixtures, monitored for 120 h, were analysed by high-performance liquid chromatography.201121800322
volatiles that encode host-plant quality in the grapevine moth.plant volatiles are signals used by herbivorous insects to locate host plants and select oviposition sites. whether such volatiles are used as indicators of plant quality by adult insects in search of host plants has been rarely tested. we tested whether volatiles indicate plant quality by studying the oviposition of the grapevine moth lobesia botrana on the grapevine plant vitis vinifera. host plants were infected with a variety of microorganisms, and larval fitness was correlated to the infect ...201121794883
investigations on the leaf anatomy and ultrastructure of grapevine (vitis vinifera) under heat stress.leaf anatomical and ultrastructural responses of "razegui" and "muscat italia" grapevine cultivars to high temperatures were studied under controlled conditions (t > 36°c), based on photonic and electron microscopy. histological studies performed on leaves from heat-stressed and control grapevines revealed thicker leaf blades under high temperature conditions. environmental scanning electron microscopy of leaf surfaces from both cultivars allowed observing sinuate epidermal cells on the leaves o ...201121780249
effects of elicitors on the production of resveratrol and viniferins in cell cultures of vitis vinifera l. cv italia.methyl jasmonate, jasmonic acid and chitosan were tested as elicitors on cell suspension cultures obtained from vitis vinifera cv italia to investigate their effect on stilbene production. stilbene accumulation in the callus, grown under nonelicited conditions, was also investigated. calli and cell suspensions were obtained in a b5 culture medium supplemented with 0.2 mg l(-1) naa and 1 mg l(-1) kin. stilbene determination was achieved by hplc/dad/ms. whereas callus biosynthesized only piceid, c ...201121751812
identification of isomers of resveratrol dimer and their analogues from wine grapes by hplc/ms(n) and hplc/dad-uv.a facile method based on hplc/(-)esi-ms(n) is established for the analysis of seven isomers of resveratrol dimers and three of their analogues in xinjiang wine grapes. the structures of these compounds are positively or tentatively determined. among them, three are tentatively identified as new compounds. ms(n) experiments on the [m-h](-) ions provide abundant structural information, especially regarding the relative abundance of the key product ions, m/z 333 and 369 (385 in compound 3), which c ...201123140727
impact of cover crops in vineyard on the aroma compounds of vitis vinifera l. cv cabernet sauvignon wine.this study compared the influence of different cover crops with clean tillage on wine aroma compounds of 5-year-old cabernet sauvignon vines. white clover, alfalfa, and tall fescue were used in the vineyard and compared with clean tillage. aroma compounds of wine were analysed by solid-phase microextraction gas chromatography-mass spectrometry (spme-gc/ms). forty-seven volatile compounds were identified and quantified. wines made from grapes grown with various cover crops had higher levels of ar ...201123140695
host plant volatiles induce oriented flight behaviour in male european grapevine moths, lobesia botrana.the european grapevine moth lobesia botrana relies on a female produced sex pheromone for long-distance mate finding. grapevine moth males compete heavily during limited time windows for females. the aim of this study was to investigate the perception of host plant volatiles by grapevine moth males and whether such compounds elicit upwind oriented flights. we compared five host plant headspace extracts by means of gas chromatography linked electroantennogram (eag) recording. we identified 12 com ...201121729701
determination of the geographical origin of brazilian wines by isotope and mineral analysis.in the present research, we studied wines from three different south brazilian winemaking regions with the purpose of differentiating them by geographical origin of the grapes. brazil's wide territory and climate diversity allow grape cultivation and winemaking in many regions of different and unique characteristics. the wine grape cultivation for winemaking concentrates in the south region, mainly in the serra gaúcha, the mountain area of the state of rio grande do sul, which is responsible for ...201121720735
the susceptibility of small fruits and cherries to the spotted-wing drosophila, drosophila suzukii.the spotted-wing drosophila, drosophila suzukii matsumura, is native to asia and was first detected in the north american mainland and europe in 2008-2010. drosophila suzukii is a serious economic pest to stone and small fruits because the female lays eggs within ripening fruit on a plant before harvest, which can lead to crop loss. the aim of this study was to evaluate the susceptibility of blackberries, blueberries, cherries, grapes, raspberries and strawberries to d. suzukii among various rip ...201121710685
mehercules, adhuc bacchus! the debate on wine proteomics continues.the proteome of untreated white wines (a recioto made with garganega grapes from the veneto region) was explored in depth via capture with combinatorial peptide ligand libraries (cpll) at four different ph values: ph 2.2, 3.8, 7.2, and 9.3. the combined data on the discoveries in the four cpll eluates, as well as in the collected bottle sediment, allowed the identification of 106 unique gene products belonging to vitis vinifera, as well as of an additional 11 proteins released by the s. cerevisi ...201121702502
relationship between red wine grade and phenolics. 2. tannin composition and size.commercial red wines ( vitis vinifera l. cv. shiraz) produced during the 2009 vintage underwent winemaker assessment for allocation grade soon after production. the wines were then subjected to phenolic analysis to measure wine color (total anthocyanin, so(2) nonbleachable pigment, and wine color density) and tannins (concentration, composition, and average degree of polymerization). a positive relationship was found between wine phenolic concentration and projected bottle price. tannin composit ...201121702478
cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine vitis vinifera.dihydrodipicolinate synthase (dhdps) catalyses the first committed step of the lysine-biosynthesis pathway in bacteria, plants and some fungi. this study describes the cloning, expression, purification and crystallization of dhdps from the grapevine vitis vinifera (vv-dhdps). following in-drop cleavage of the hexahistidine tag, cocrystals of vv-dhdps with the substrate pyruvate were grown in 0.1 m bis-tris propane ph 8.2, 0.2 m sodium bromide, 20%(w/v) peg 3350. x-ray diffraction data in space g ...201122139160
evaluation of genetic dissimilarity in a segregating wine grape population.this study examined the genetic diversity present in a population obtained by crossing two very distinctive varieties of wine grapes (142 progeny from a riesling x cabernet sauvignon cross, including both parents, created at the university of california). the following list of morphological characters were evaluated and found to segregate in this population: cluster weight, cluster length, presence or absence of cluster wings, cluster wing length, number of berries, cluster density (cd), and ber ...201122095478
detailed characterization of proanthocyanidins in skin, seeds, and wine of shiraz and cabernet sauvignon wine grapes (vitis vinifera).the distribution of proanthocyanidin (pa) polymer lengths, proanthocyanidin concentration at each polymer length, and polymer composition were determined in the seed, skin, and wine of shiraz and cabernet sauvignon grape berries grown in southeast australia. pa was fractionated by semipreparative high performance liquid chromatography (hplc) and analyzed by phloroglucinolysis and hplc to report the degree of polymerization (dp), concentration, and composition at 11 dp values in seed and wine and ...201122085086
a 48 snp set for grapevine cultivar identification.rapid and consistent genotyping is an important requirement for cultivar identification in many crop species. among them grapevine cultivars have been the subject of multiple studies given the large number of synonyms and homonyms generated during many centuries of vegetative multiplication and exchange. simple sequence repeat (ssr) markers have been preferred until now because of their high level of polymorphism, their codominant nature and their high profile repeatability. however, the rapid a ...201122060012
evaluation of differences in the aroma composition of free-run and pressed neutral grape juices obtained from emir (vitis vinifera l.).in this study, the differences in the aroma compounds released after the free-run and pressed juices of cv. emir grape (vitis vinifera l.) were evaluated. aroma compounds were obtained by liquid-liquid extraction with ch(2) cl(2) , and then analyzed by gas chromatography (gc) and gas chromatography/mass spectrometry (gc/ms). according to the results, pressing uniformly increased the levels of the aromatic constituents, but this treatment lowered the grape juice quality for winemaking by increasi ...201121922666
seasonal fluctuations in vitis vinifera root respiration in the field.we studied the seasonal fluctuation of soil respiration (r(s)), and its root-dependent (r(r)) and basal (r(b)) components, in a vitis vinifera (chardonnay) vineyard. the r(s) components were estimated through independent field methods (y-intercept and trenching) and modeled on the basis of a q(10) response to soil temperature, and fine and coarse root respiration coefficients. the effect of assimilate availability on r(r) was assessed through a trunk girdling treatment. the apparent q(10) for r( ...201121919911
antioxidant activity of grape skin aqueous extracts from pressurized hot water extraction combined with electron paramagnetic resonance spectroscopy.pressurized hot water extraction (phwe) was employed to prepare extracts from dried grape skin of two wine grape varieties (st. laurent and alibernet) at various temperatures (from 40 up to 120°c) and amounts of sample (0.5, 1.0 and 1.5 g). to assess the antioxidant activity of the extracts, electron paramagnetic resonance (epr) spectroscopy was applied involving dpph and abts(+) assays. other extract characteristics including hplc profile of anthocyanins and total phenolic compound content were ...201121872083
multivariate analysis of the polyphenol composition of tempranillo and graciano red wines.vitis vinifera l. cv graciano is often used as a blending partner of tempranillo based wines because it is considered to contribute significantly to the quality. the aim of this study is to discriminate between tempranillo and graciano monovarietal wines, and those made by the incorporation of 20% of graciano variety in two different stages (at the end of malolactic fermentation and mixing the two grape varieties in the pre-fermentative maceration stage) of the winemaking process of the temprani ...201121872058
horam nonam exclamavit: sitio. the trace proteome of your daily vinegar.the trace proteome of white-wine vinegar has been identified via capture with home-made combinatorial peptide ligand libraries under conditions mimicking reverse-phase capture, i.e. at ph 2.2 in presence of 0.1% trifluoroacetic acid. a total of 27 unique gene products have been identified, of which 10 specific of the database vitis vinifera, 13 found in the general database uniprot_viridiplantae and 4 in swiss prot_all entries. the most abundant species detected, on the basis of spectral counts, ...201121867782
occurrence, sensory impact, formation, and fate of damascenone in grapes, wines, and other foods and beverages.among plant-derived odorants, damascenone is one of the most ubiquitous, sometimes occurring as an apparent natural product but more commonly occurring in processed foodstuffs and beverages. it has been widely reported as a component of alcoholic beverages, particularly of wines made from the grape vitis vinifera . although damascenone has one of the lowest ortho- and retronasal detection thresholds of any odorant, its contribution to the sensory properties of most products remains poorly unders ...201121866982
varietal relationship between instrumental skin hardness and climate for grapevines (vitis vinifera l.).the main aims of this work were to classify 30 colored and white wine grape varieties according to the berry skin hardness, to assess the influence of annual variations in climate on the berry skin hardness, and to establish significant relationships among berry skin mechanical properties and some climatic-bioclimatic indices calculated for different grape-ripening periods, close to the harvest date. the results obtained show that the most influential bioclimatic indices on the skin mechanical a ...201121861480
phenolics and antioxidant capacity of table grape (vitis vinifera l.) cultivars grown in chile.grapes (vitis vinifera l.) possess health-promoting effects attributed to their supply of a wide variety of bioactive phenolics. juice and skin fractions of 4 varieties of table grapes: red globe, crimson seedless, autumn royal, and ribier were prepared to determine and compare their total phenolics content, antioxidant capacity (dpph, frap, and orac), anthocyanins, and specific phenolics (caffeic acid, gallic acid, resveratrol, and catechin) content, since a series of positive health benefits a ...201121819404
optimisation of solid-phase microextraction combined with gas chromatography-mass spectrometry based methodology to establish the global volatile signature in pulp and skin of vitis vinifera l. grape varieties.the volatiles (vocs) and semi-volatile organic compounds (svocs) responsible for aroma are mainly present in skin of grape varieties. thus, the present investigation is directed towards the optimisation of a solvent free methodology based on headspace-solid-phase microextraction (hs-spme) combined with gas chromatography-quadrupole mass spectrometry (gc-qms) in order to establish the global volatile composition in pulp and skin of bual and bastardo vitis vinifera l. varieties. a deep study on th ...201121807213
unsupervised principal component analysis of nmr metabolic profiles for the assessment of substantial equivalence of transgenic grapes (vitis vinifera).substantial equivalence is a key concept in the evaluation of unintended and potentially harmful metabolic impact consequent to a genetic modification of food. the application of unsupervised multivariate data analysis to the metabolic profiles is expected to improve the effectiveness of such evaluation. the present study uses nmr spectra of hydroalcoholic extracts, as holistic representations of the metabolic profiles of grapes, to evaluate the effect of the insertion of one or three copies of ...201121806070
the presence of melatonin in grapevine (vitis vinifera l.) berry tissues.melatonin has been reported in a variety of food plants and, consequently, in a number of plant-derived foodstuffs. in grapevine (vitis vinifera l.) products, it was found in berry exocarp (skin) of different cultivars and monovarietal wines. herein, we assessed, by means of mass spectrometry, the occurrence of melatonin in all berry tissues (skin, flesh, and seed) at two different phenological stages, pre-véraison and véraison. we detected the highest melatonin content in skin, at pre-véraison, ...201121615489
in vivo grapevine anthocyanin transport involves vesicle-mediated trafficking and the contribution of anthomate transporters and gst.in cells, anthocyanin pigments are synthesized at the cytoplasmic surface of the endoplasmic reticulum, and are then transported and finally accumulated inside the vacuole. in vitis vinifera (grapevine), two kinds of molecular actors are putatively associated with the vacuolar sequestration of anthocyanins: a glutathione-s-transferase (gst) and two mate-type transporters, named anthomates. however, the sequence of events by which anthocyanins are imported into the vacuole remains unclear. we use ...201121605207
melatonin levels, determined by lc-esi-ms/ms, fluctuate during the day/night cycle in vitis vinifera cv malbec: evidence of its antioxidant role in fruits.the identification of melatonin in plants has inspired new investigations to understand its biological function and which endogenous and external factors control its levels in these organisms. owing to the therapeutical and nutraceutical properties of melatonin, it should be important to develop reliable analytical methods for its quantification in vegetal matrices containing this indoleamine, such as grape and wine. the main objectives of the present study were to test whether melatonin levels ...201121605162
biosynthesis of methoxypyrazines: elucidating the structural/functional relationship of two vitis viniferao-methyltransferases capable of catalyzing the putative final step of the biosynthesis of 3-alkyl-2-methoxypyrazine.3-alkyl-2-methoxypyrazines (mps) are an important food constituent and have been associated with detrimental herbaceous flavors in red wines by consumers and the wine industry. the vitis vinifera genes o-methyltransferase 1 and 2 (vvomt1 and vvomt2) have been isolated in the grapevine cultivar carmenere. these genes encode s-adenosyl-l-methionine (sam)-dependent o-methyltransferases, which have the ability to methylate 3-alkyl-2-hydroxypyrazines (hps)-the putative final step in mps production. a ...201121591781
fruit ripening in vitis vinifera: spatiotemporal relationships among turgor, sugar accumulation, and anthocyanin biosynthesis.this study reports the first observations indicating the spatiotemporal relationships among genetic and physiological aspects of ripening in the berry of vitis vinifera. at the onset of ripening in the red flesh variety alicante bouschet, colour development began in the flesh at the stylar end of the fruit and progressed toward the pedicel end flesh and into the skin. tissue solute potential and cell turgor also decreased first in the flesh. the decrease in flesh solute potential was due to accu ...201121586429
from vineyard to glass: agrochemicals enhance the melatonin and total polyphenol contents and antiradical activity of red wines.resistance inducers are a class of agrochemicals, including benzothiadiazole and chitosan, which activate the plant own defence mechanisms. in this work, open-field treatments with plant activators were performed on two red grape (vitis vinifera l.) varieties cultivated in different localities, groppello (brescia, lombardia, italy) and merlot (treviso, veneto, italy). treatments were carried out every 10 days until the véraison and, after harvesting, experimental wines (microvinificates) were pr ...201121585520
a dige-based quantitative proteomic analysis of grape berry flesh development and ripening reveals key events in sugar and organic acid metabolism.grapevine (vitis vinifera l.) is an economically important fruit crop. quality-determining grape components, such as sugars, acids, flavours, anthocyanins, tannins, etc., are accumulated during the different grape berry development stages. thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in grape is of paramount importance to advance the understanding of the berry development and ripening processes. here, the developmental analysis of v. vinifera ...201121576399
automated analysis of three-dimensional xylem networks using high-resolution computed tomography.connections between xylem vessels represent important links in the vascular network, but the complexity of three-dimensional (3d) organization has been difficult to access. this study describes the development of a custom software package called tanax (tomography-derived automated network analysis of xylem) that automatically extracts vessel dimensions and the distribution of intervessel connections from high-resolution computed tomography scans of grapevine (vitis vinifera) stems, although the ...201121569032
inflorescence of grapevine (vitis vinifera l.): a high ability to distribute its own assimilates.the distribution of carbon (c) into whole grapevine fruiting cuttings was investigated during flower development to determine the relative contribution of inflorescence and leaf photoassimilates in the total c balance and to investigate their partitioning towards other plant organs. a (13)c labelling procedure was used to label c photoassimilates by leaves and inflorescences in grapevine. investigations were carried out at various stages of flower/berry development, from separated cluster to fru ...201121561954
evolution of phenolic composition of red wine during vinification and storage and its contribution to wine sensory properties and antioxidant activity.the objective of this work was to study the evolution of the phenolic composition of red wine during vinification and storage and its relationship with some sensory properties (astringency and bitterness) and antioxidant activities. thus, red wine was made by a classic vinification method with castelão and tinta miúda grapes (vitis vinifera l.) harvested at maturity (3:2; w/w). samples were taken at 2 and 7 days of maceration, at second racking, at the time of bottling and at 6 and 14 months aft ...201121561162
acyl substrate preferences of an iaa-amido synthetase account for variations in grape (vitis vinifera l.) berry ripening caused by different auxinic compounds indicating the importance of auxin conjugation in plant development.nine gretchen hagen (gh3) genes were identified in grapevine (vitis vinifera l.) and six of these were predicted on the basis of protein sequence similarity to act as indole-3-acetic acid (iaa)-amido synthetases. the activity of these enzymes is thought to be important in controlling free iaa levels and one auxin-inducible grapevine gh3 protein, gh3-1, has previously been implicated in the berry ripening process. ex planta assays showed that the expression of only one other gh3 gene, gh3-2, incr ...201121543520
phenolic composition of malbec grape skins and seeds from valle de uco (mendoza, argentina) during ripening. effect of cluster thinning.the phenolic composition of malbec (vitis vinifera l.) grape skins and seeds during ripening and the effect of cluster thinning (ct) in two consecutive seasons (2008-2009) were evaluated by high-performance liquid chromatography-diode array detection/electrospray ionization-mass spectrometry (hplc-dad/esi-ms). removal of 50% of clusters was performed at 40 days (t1), 80 days (t2), and 100 days after flowering (t3) in a vineyard located in southern mendoza (argentina). yield components, with the ...201121520971
comparative transcriptomic profiling of vitis vinifera under high light using a custom-made array and the affymetrix genechip.understanding abiotic stress responses is one of the most important issues in plant research nowadays. abiotic stress, including excess light, can promote the onset of oxidative stress through the accumulation of reactive oxygen species. oxidative stress also arises when in vitro propagated plants are exposed to high light upon transfer to ex vitro. to determine whether the underlying pathways activated at the transfer of in vitro grapevine to ex vitro conditions reflect the processes occurring ...201121498622
effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine.in the present work, the effect of assisted fertilization on anatomical, morphological and gene expression changes occurring in carpels and during early stages of berry development in vitis vinifera were studied. inflorescences were emasculated before capfall, immediately manually pollinated (ep) and fruit development was compared to emasculated but non-pollinated (enp) and self-pollinated inflorescences (nesp). the diameter of berries derived from pollinated flowers (ep and nesp) was significan ...201121497942
deep sequencing of grapevine flower and berry short rna library for discovery of novel micrornas and validation of precise sequences of grapevine micrornas deposited in mirbase.micrornas (mirnas) are a class of non-coding rna molecules which have significant gene regulation roles in organisms. the advent of new high-throughput sequencing technologies has enabled the discovery of novel mirnas. although there are two recent reports on high-throughput sequencing analysis of small rna libraries from different organs of two wine grapevine varieties, there was a significant divergence in the number and kinds of mirnas sequenced in these studies. more sequencing of small rna ...201121496033
determination of stilbene derivatives in burgundy red wines by ultra-high-pressure liquid chromatography.the polyphenols, for example stilbenes and flavonoids, are an important family of compounds present in grapes and wines. several studies have shown that stilbenes are antioxidants and cancer-preventing agents. for the first time, eight natural stilbenes (trans-resveratrol, trans-piceid, cis-piceid, trans-astringin, trans-piceatannol, (+)-trans-ε-viniferin, pallidol, and hopeaphenol), isolated and purified from vitis vinifera, were simultaneously analysed by ultra-high-pressure liquid chromatogra ...201121487710
pectin-derived oligosaccharides increase color and anthocyanin content in flame seedless grapes.grapes grown in warm weather do not develop a desirable red color and require the use of products to enhance berry color. pectin-derived oligosaccharides (pdos) have been shown to have a role in various responses including plant defense, growth and development. in this work a mixture of pdos with 3-20 degrees of polymerization was applied to vitis vinifera cv. flame seedless grapes under field conditions and compared to the effects of ethephon (an ethylene-releasing compound). the effect of trea ...201121484810
solar uv-b and aba are involved in phenol metabolism of vitis vinifera l. increasing biosynthesis of berry skin polyphenols.it has been previously found that abscisic acid (aba) participates in the activation of grapevine leaf tissue defense against potentially damaging effects of solar ultraviolet-b radiation (uv-b), apparently by triggering biosynthesis of phenols that filter the harmful radiation and act as antioxidants. the present work studies the effect of solar uv-b and exogenously applied aba on berry growth, sugar accumulation, and phenol (anthocyanin and nonanthocyanin) profiles across berry development and ...201121469737
occurrence and estimation of trans-resveratrol in one-year-old canes from seven major chinese grape producing regions.the concentration of trans-resveratrol in 165 grape cane samples from three major grape production regions and four large distribution centers of chinese wild vitis species were determined by reversed-phase high-performance liquid chromatography (hplc). among the different genotype groups and purpose of uses, cultivars of v. vinifera had much higher amounts of trans-resveratrol than did the cultivars of both v. labrusca or v. labrusca and v. vinifera hybrids, and within the v. vinifera species, ...201121455097
expression analysis of phytochromes a, b and floral integrator genes during the entry and exit of grapevine-buds from endodormancy.a common molecular regulatory pathway that involves phya, phyb and floral integrator genes constans (co), flowering locus t (ft) and supressor of overexpression of co1 (soc1) has been suggested to participate in the regulation of photoperiod dependent processes such as flowering and dormancy. in grapevines (vitis vinifera l.), decreasing photoperiod and low temperatures trigger the transition of buds into endodormancy (ed), a process that is accompanied by drastic changes in gene expression of v ...201121453983
diurnal cycles of embolism formation and repair in petioles of grapevine (vitis vinifera cv. chasselas).the impact of water deficit on stomatal conductance (g(s)), petiole hydraulic conductance (k(petiole)), and vulnerability to cavitation (plc, percentage loss of hydraulic conductivity) in leaf petioles has been observed on field-grown vines (vitis vinifera l. cv. chasselas). petioles were highly vulnerable to cavitation, with a 50% loss of hydraulic conductivity at a stem xylem water potential (ψ(x)) of -0.95 mpa, and up to 90% loss of conductivity at a ψ(x) of -1.5 mpa. k(petiole) described a d ...201121447755
molecular, genetic and transcriptional evidence for a role of vvagl11 in stenospermocarpic seedlessness in grapevine.stenospermocarpy is a mechanism through which certain genotypes of vitis vinifera l. such as sultanina produce berries with seeds reduced in size. stenospermocarpy has not yet been characterized at the molecular level.201121447172
dige analysis of proteome changes accompanying large resveratrol production by grapevine (vitis vinifera cv. gamay) cell cultures in response to methyl-β-cyclodextrin and methyl jasmonate elicitors.we had previously shown that vitis vinifera cv. gamay cell suspension accumulates extracellularly large amounts of the phytoalexin trans-resveratrol (tr) in response to elicitation with methylated cyclodextrins (mbcd), which can be triplicated when the elicitor is combined with methyl jasmonate (meja). in parallel, new pathogenesis-related proteins accumulated in the apoplast-like extracellular space. the aim of this study was to investigate changes in the grapevine cell proteome potentially rel ...201121426946
proteomic analysis of grape berry cell cultures reveals that developmentally regulated ripening related processes can be studied using cultured cells.this work describes a proteomics profiling method, optimized and applied to berry cell suspensions to evaluate organ-specific cultures as a platform to study grape berry ripening. variations in berry ripening within a cluster(s) on a vine and in a vineyard are a major impediment towards complete understanding of the functional processes that control ripening, specifically when a characterized and homogenous sample is required. berry cell suspensions could overcome some of these problems, but the ...201121379583
increased accumulation and decreased catabolism of anthocyanins in red grape cell suspension culture following magnesium treatment.anthocyanins are the largest and best studied group of plant pigments. however, not very much is known about the fate of these phenolic pigments after they have accumulated in the cell vacuoles of plant tissues. we have previously shown that magnesium treatment of ornamentals during the synthesis of anthocyanins in the flowers or foliage caused an increase in the pigment concentration. in this study, we characterized the effect of magnesium on the accumulation of anthocyanin in red cell suspensi ...201121369922
changes of platelet antioxidative enzymes during oxidative stress: the protective effect of polyphenol-rich extract from berries of aronia melanocarpa and grape seeds.aronia melanocarpa fruits (rosaceae) and grape seeds (seeds of vitis vinifera, vitaceae) are two of the richest plant sources of phenolic substances, and they have been shown to have various biological activities. the aim of the present study was to investigate and compare the action of phenolic extracts (at concentrations 5-100 µg/ml) of two different plants, berries of a. melanocarpa (chokebbery) and grape seeds, on the activities of various antioxidative enzymes, the amount of glutathione (as ...201121299394
genetic structure and domestication history of the grape.the grape is one of the earliest domesticated fruit crops and, since antiquity, it has been widely cultivated and prized for its fruit and wine. here, we characterize genome-wide patterns of genetic variation in over 1,000 samples of the domesticated grape, vitis vinifera subsp. vinifera, and its wild relative, v. vinifera subsp. sylvestris from the us department of agriculture grape germplasm collection. we find support for a near east origin of vinifera and present evidence of introgression fr ...201121245334
high throughput analysis of grape genetic diversity as a tool for germplasm collection management.using 20 ssr markers well scattered across the 19 grape chromosomes, we analyzed 4,370 accessions of the inra grape repository at vassal, mostly cultivars of vitis vinifera subsp. sativa (3,727), but also accessions of v. vinifera subsp. sylvestris (80), interspecific hybrids (364), and rootstocks (199). the analysis revealed 2,836 ssr single profiles: 2,323 sativa cultivars, 72 wild individuals (sylvestris), 306 interspecific hybrids, and 135 rootstocks, corresponding to 2,739 different cultiva ...201121234742
berry skin development in norton grape: distinct patterns of transcriptional regulation and flavonoid biosynthesis.the complex and dynamic changes during grape berry development have been studied in vitis vinifera, but little is known about these processes in other vitis species. the grape variety 'norton', with a major portion of its genome derived from vitis aestivalis, maintains high levels of malic acid and phenolic acids in the ripening berries in comparison with v. vinifera varieties such as cabernet sauvignon. furthermore, norton berries develop a remarkably high level of resistance to most fungal pat ...201121219654
influence of skin hardness on dehydration kinetics of wine grapes.knowledge of the influence of initial mechanical properties on the evolution of the weight loss of berries through the drying process is scarce. therefore, the main purpose of this work was to investigate the effect of skin hardness at two different physiological stages of off-vine drying kinetics of grapes. skin hardness was evaluated as the berry skin-break force parameter, measured by the texture analysis test.201121218485
new phenolic grape skin products from vitis vinifera cv. pinot noir.anthocyanins and their related compounds were extracted from grape skins of pinot noir, using 50% aqueous methanol, and purified by solid phase extraction chromatography using xad-7 resin to obtain a pigment-rich fraction. this fraction was subjected to multilayer coil countercurrent chromatography (mlccc) using a quaternary solvent system consisting of tert-butyl methyl ether/n-butanol/acetonitrile/water acidified with 0.01% trifluoroacetic acid (2:2:0.1-1.8:5) (v/v/v/v) in a step gradient elut ...201121214245
reductions in biomass accumulation, photosynthesis in situ and net carbon balance are the costs of protecting vitis vinifera 'semillon' grapevines from heat stress with shade covering.covering whole vines with shade cloth is used to protect the vines from heat stress, but may have costs on vine productivity through reduced light availability. our aim was to assess the carbon balance of vines growing with and without shade to quantify the impact of the covering.201122476493
effects of pulsed electric field on secondary metabolism of vitis vinifera l. cv. gamay fréaux suspension culture and exudates.plant cell cultures provide a large potential for the production of secondary metabolites. through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. the effects of pulsed electric field (pef) and ethephon on growth and secondary metabolism, particularly anthocyanins and phenolic acids synthesis, were investigated by using suspension culture of vitis vinifera l. cv. gamay fréaux as a model sy ...201121190090
low-temperature-induced transcription factors in grapevine enhance cold tolerance in transgenic arabidopsis plants.we report the characterization of low-temperature-induced transcription factors in grapevine (vitis vinifera). four transcription factors were identified in low-temperature-treated grapevine. the expression of v. vinifera c-repeat-binding factors, vvcbf2, vvcbf4, and vvcbfl, and v. vinifera b-box-type zinc finger protein, vvzfpl, was immediately induced and upregulated in leaves by the low-temperature treatment. similar induction of the gene expression was observed in low-temperature-treated ste ...201121185622
genetic mechanisms underlying the methylation level of anthocyanins in grape (vitis vinifera l.).plant color variation is due not only to the global pigment concentration but also to the proportion of different types of pigment. variation in the color spectrum may arise from secondary modifications, such as hydroxylation and methylation, affecting the chromatic properties of pigments. in grapes (vitis vinifera l.), the level of methylation modifies the stability and reactivity of anthocyanin, which directly influence the color of the berry. anthocyanin methylation, as a complex trait, is co ...201122171701
increasing the source/sink ratio in vitis vinifera (cv sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening.cluster thinning is an agronomic practice in which a proportion of berry clusters are removed from the vine to increase the source/sink ratio and improve the quality of the remaining berries. until now no transcriptomic data have been reported describing the mechanisms that underlie the agronomic and biochemical effects of thinning.201122192855
the hepatoprotective effects of dihydromyrcenol and geranyl formate in an experimental model of acute hepatic injury induced by the use of carbon tetrachloride.we aimed to investigate the hepatoprotective effects of dihydromyrcenol and geranyl formate extracted from the vitis vinifera l. plant in a rat model of acute hepatic injury induced by carbon tetrachloride.201122287404
optimization of nir spectral data management for quality control of grape bunches during on-vine ripening.nir spectroscopy was used as a non-destructive technique for the assessment of chemical changes in the main internal quality properties of wine grapes (vitis vinifera l.) during on-vine ripening and at harvest. a total of 363 samples from 25 white and red grape varieties were used to construct quality-prediction models based on reference data and on nir spectral data obtained using a commercially-available diode-array spectrophotometer (380-1,700 nm). the feasibility of testing bunches of intact ...201122163944
polyphenolic profiles detected in the ripe berries of vitis vinifera germplasm.polyphenolic profiles in the berry samples of 344 european grape (vitis vinifera) cultivars were evaluated for two consecutive years. these cultivars represent a diverse collection of v. vinifera germplasm maintained at the usda-agricultural research service vitis clonal repository in davis of california, usa. a total of 36 polyphenolic compounds, including 16 anthocyanins, 6 flavonols, 6 flavanols, 6 hydroxycinnamic acids and 2 hydroxybenzoic acids, were identified via hplc-ms and quantified by ...201125212322
relationships between godello white wine sensory properties and its aromatic fingerprinting obtained by gc-ms.godello is a traditional white-grape vine grown widely in the valdeorras area (northwest spain) available information about the sensory and chemical characteristics of which is scant. in this work, exploratory research was conducted with a view to correlate the results of instrumental analyses of the aroma compounds in godello wine and their sensory perception. principal component analysis (pca) and partial least squares regression (pls) were the multivariate data analysis methods selected. pca ...201125212315
antioxidant activities, sensory and anti-influenza activity of grape skin tea infusion.grape skin extracts from pinot noir and pinot gris exhibited significant in vitro antiviral (influenza virus) activity. five tea infusions from grape skins (vitis vinifera var. pinot noir and pinot gris) without any additives (control pinot noir and control pinot gris) or by adding variable amounts of green tea and hibiscus were investigated as a means to utilise wine wastes. the antioxidant activities (dpph scavenging capacity and superoxide anion radical scavenging capacity), total phenolics, ...201125212307
transcript and metabolite analysis in trincadeira cultivar reveals novel information regarding the dynamics of grape ripening.grapes (vitis vinifera l.) are economically the most important fruit crop worldwide. however, the complexity of molecular and biochemical events that lead to the onset of ripening of nonclimacteric fruits is not fully understood which is further complicated in grapes due to seasonal and cultivar specific variation. the portuguese wine variety trincadeira gives rise to high quality wines but presents extremely irregular berry ripening among seasons probably due to high susceptibility to abiotic a ...201122047180
optimization, in-house validation, and application of a liquid chromatography-tandem mass spectrometry (lc-ms/ms)-based method for the quantification of selected polyphenolic compounds in leaves of grapevine (vitis vinifera l.).polyphenols in grapevine can be constitutive or induced, depending upon cultivar, plant organ, and environmental influences. the aim of the presented work was to develop and optimize a liquid chromatography-tandem mass spectrometry (lc-ms/ms) method to study the pattern and amount of selected polyphenols in leaves of vitis vinifera l. the method is simple and does not require any sample cleanup. it covers representative metabolites of the structure classes cinnamic acids, flavonoids, and stilben ...201121910493
chemical ecology and management of lobesia botrana (lepidoptera: tortricidae).the moth lobesia botrana (denis & schiffermüller) (lepidoptera: tortricidae) feeds on grapes (vitis vinifera l.), reducing yield and increasing susceptibility to fungal infections. l. botrana is among the most economically important insects in europe and has recently been found in vineyards in chile, argentina, and california. here, we review l. botrana biology and behavior in relation to its larval host (the grapevine) and its natural enemies. we also discuss current and future control strategi ...201121882674
instability of anthocyanin composition under different subculture conditions during long-term suspension cultures of vitis vinifera l. var. gamay fréaux.the instability of secondary metabolite production is a ubiquitous problem in plant cell culture. in order to understand the instability in plant cell culture, we investigated anthocyanin accumulation in suspension cultures of vitis vinifera, as a model system, in our laboratory. not only the anthocyanin contents but also its composition exhibited instability along with the long-term subculture. new methods were developed to indicate the instability of plant cell culture. both the definition of ...201122393716
profiling of resveratrol oligomers, important stress metabolites, accumulating in the leaves of hybrid vitis vinifera (merzling × teroldego) genotypes infected with plasmopara viticola.in the vitaceae, viniferins represent a relatively restricted group of trans-resveratrol oligomers with antifungal properties, thus enabling plants to cope with pathogen attack. the aim of this study was to perform isolation and structural characterization of the whole class of viniferins accumulating in the leaves of hybrid vitis vinifera (merzling × teroldego) genotypes infected with plasmopara viticola . infected leaves of resistant plants were collected 6 days after infection, extracted with ...201121510709
a white spruce gene catalog for conifer genome analyses.several angiosperm plant genomes, including arabidopsis (arabidopsis thaliana), rice (oryza sativa), poplar (populus trichocarpa), and grapevine (vitis vinifera), have been sequenced, but the lack of reference genomes in gymnosperm phyla reduces our understanding of plant evolution and restricts the potential impacts of genomics research. a gene catalog was developed for the conifer tree picea glauca (white spruce) through large-scale expressed sequence tag sequencing and full-length cdna sequen ...201121730200
analysis of bac end sequences in oak, a keystone forest tree species, providing insight into the composition of its genome.one of the key goals of oak genomics research is to identify genes of adaptive significance. this information may help to improve the conservation of adaptive genetic variation and the management of forests to increase their health and productivity. deep-coverage large-insert genomic libraries are a crucial tool for attaining this objective. we report herein the construction of a bac library for quercus robur, its characterization and an analysis of bac end sequences.201121645357
a physical map for the amborella trichopoda genome sheds light on the evolution of angiosperm genome structure.recent phylogenetic analyses have identified amborella trichopoda, an understory tree species endemic to the forests of new caledonia, as sister to a clade including all other known flowering plant species. the amborella genome is a unique reference for understanding the evolution of angiosperm genomes because it can serve as an outgroup to root comparative analyses. a physical map, bac end sequences and sample shotgun sequences provide a first view of the 870 mbp amborella genome.201121619600
fructo-oligosaccharides in table grapes and response to storage.fructo-oligosaccharides (fos) have been recognized as health food ingredients with a protective effect against environmental stresses in plants. we have analyzed the profiles of individual fos in cardinal table grape pulp, until now undetected, and quantified their changes in response to low temperature and high co2 levels. fos separation and quantification was carried out using anion-exchange chromatography with pulsed amperometric detection (hpaec-pad), and the glucose, fructose and sucrose co ...201125212291
influence of foliar fertilization with p and k on chemical constituents of grape cv. 'cardinal'.the foliar fertilization has been used as an important agrotechnical measure to avoid deficiencies and to improve quality. during the two consecutive years, a study has been performed on vitis vinifera l. (cv. 'cardinal') to examine whether a grape berry quality has been affected by the foliar application of pk fertilizer. a liquid mineral fertilizer containing 15% p2o5, 20% k2o with 0.1% b, 0.1% mn and 0.01% mo (% w/w) has been sprayed three times at rate of 8 l ha(-1) every 14-15 days starting ...201121827138
expression and genome-wide analysis of the xylogen-type gene family.in higher plants, many extracellular proteins are involved in developmental processes, including cell-cell signaling and cell wall construction. xylogen is an extracellular arabinogalactan protein (agp) isolated from zinnia elegans xylogenic culture medium, which promotes xylem cell differentiation. xylogen has a unique structure, containing a non-specific lipid transfer protein (nsltp) domain and agp domains. we searched for xylogen-type genes in the genomes of land plants, including arabidopsi ...201121558309
exploration of plant genomes in the flagdb++ environment.in the contexts of genomics, post-genomics and systems biology approaches, data integration presents a major concern. databases provide crucial solutions: they store, organize and allow information to be queried, they enhance the visibility of newly produced data by comparing them with previously published results, and facilitate the exploration and development of both existing hypotheses and new ideas.201121447150
comparative analysis of gossypium and vitis genomes indicates genome duplication specific to the gossypium lineage.genetic mapping studies have suggested that diploid cotton (gossypium) might be an ancient polyploid. however, further evidence is lacking due to the complexity of the genome and the lack of sequence resources. here, we used the grape (vitis vinifera) genome as an out-group in two different approaches to further explore evidence regarding ancient genome duplication (wgd) event(s) in the diploid gossypium lineage and its (their) effects: a genome-level alignment analysis and a local-level sequenc ...201121352905
molecular cloning and characterization of ghapm, a gene encoding the μ subunit of the clathrin-associated adaptor protein complex that is associated with cotton (gossypium hirsutum) fiber development.the clathrin-associated adaptor protein (ap) complexes are the primary clathrin adaptors that contribute to the formation of clathrin-coated vesicles (ccvs). the ghapm gene (genbank accession number: gu359054), which encodes the medium subunit of the ap complexes, was cloned from cotton by rapid amplification of cdna ends-polymerase chain reaction (race-pcr). the full-length cdna was 1590 bp in size and encoded an open reading frame (orf) of 416 amino acids with a molecular weight of 46 kda. the ...201121225463
longidorus carniolensis sp. n. (nematoda, longidoridae) from vineyard soil in slovenia.a new needle nematode, longidorus carniolensissp. n., recovered from the soil around the roots of grapevine vitis vinifera l. from slovenia, is described and illustrated. longidorus carniolensisis an amphimictic species, characterised by females with a moderately long (l=5.6-8.2 mm) and plump (a=51-72.4, ave. 66.3) body, assuming a spiral to c-shape when heat relaxed. head region continuous, anteriorly almost flat, lip region 23-25 µm wide; guiding ring situated posteriorly (42-47 μm, 43-50 μm i ...201122287876
rootstock and vineyard floor management influence on 'cabernet sauvignon' grape yeast assimilable nitrogen (yan).this is a study on the influence that two rootstocks (110r, high vigour; 420a, low vigour) and three vineyard floor management regimes (tilled resident vegetation - usual practise in california, and barley cover crops that were either mowed or tilled) had upon grape nitrogen-containing compounds (mainly ammonia and free amino acids recalculated as yan), sugars, and organic acids in 'cabernet sauvignon' clone 8. a significant difference was observed for some of the free amino acids between rootst ...201125214080
comparison of odor-active compounds in grapes and wines from vitis vinifera and non-foxy american grape species.native american grape (vitis) species have many desirable properties for winegrape breeding, but hybrids of these non-vinifera wild grapes with vitis vinifera often have undesirable aromas. other than the foxy-smelling compounds in vitis labrusca and vitis rotundifolia , the aromas inherent to american vitis species are not well characterized. in this paper, the key odorants in wine produced from the american grape species vitis riparia and vitis cinerea were characterized in comparison to wine ...201121879766
synergistic antioxidant activity of green tea with some herbs.cardiovascular diseases, cancer, arthritis, etc. are caused by free radicals that are byproducts of metabolic pathways. selected plants namely vitis vinifera, phyllanthus emblica l., punica granatum, cinnamomum cassia, ginkgo biloba l., and camellia sinensis linn. are reported to produce antioxidant property. this study is undertaken to support the hypothesis that formulation of a polyherbal combination of these plants shows a synergistic effect with green tea. the extracts of each drug were cha ...201122171315
an est-based analysis identifies new genes and reveals distinctive gene expression features of coffea arabica and coffea canephora.coffee is one of the world's most important crops; it is consumed worldwide and plays a significant role in the economy of producing countries. coffea arabica and c. canephora are responsible for 70 and 30% of commercial production, respectively. c. arabica is an allotetraploid from a recent hybridization of the diploid species, c. canephora and c. eugenioides. c. arabica has lower genetic diversity and results in a higher quality beverage than c. canephora. research initiatives have been launch ...201121303543
host plant suitability and feeding preferences of the grapevine pest abagrotis orbis (lepidoptera: noctuidae).thirteen plant species were tested for their suitability as hosts for abagrotis orbis (grote), a climbing cutworm pest of grapevines in british columbia. choice tests were also conducted to investigate larval feeding preferences for the brassicaceae species joi choi, brassica rapa variety. chinensis l., spring draba; draba verna l.; and shepherd's purse, capsella bursa-pastoris (l.) medik; compared with postdormant buds of grape, vitis vinifera l. (vitaceae), and leaves of nine other plant speci ...201122217761
new records of eriophyoid mites (acari: prostigmata: eriophyoidea) from saudi arabia.a field study was conducted to investigate eriophyoid mites associated with some fruit trees in riyadh, saudi arabia. the survey was carried out in four localities (el-waseel, al-beer, al-haiyer and el-deriya) and included seven species of fruit trees, namely olives (olea europea), fig (ficus carica), grapes (vitis vinifera), apple (malus domestica), citrus (citrus spp.), pomegranate (punica granatum) and pear (pyrus communis). seven new records of eriophyoid species (aceria benghalensis soliman ...201121916261
association of a novel dna virus with the grapevine vein-clearing and vine decline syndrome.a severe vein-clearing and vine decline syndrome has emerged on grapevines (vitis vinifera) and hybrid grape cultivars in the midwest region of the united states. the typical symptoms are translucent vein-clearing on young leaves, short internodes and decline of vine vigor. known viral pathogens of grapevines were not closely associated with the syndrome. to obtain a comprehensive profile of viruses in a diseased grapevine, small rnas were enriched and two cdna libraries were constructed from a ...201121554183
comparison of foliar and soil formulations of neonicotinoid insecticides for control of potato leafhopper, empoasca fabae (homoptera: cicadellidae), in wine grapes.the potential of systemic neonicotinoid insecticides to control potato leafhopper, empoasca fabae (harris), a damaging pest of wine grapes in the eastern united states, was investigated. soil or foliar applications were made to potted or field-grown vines, and the response of leafhoppers was determined in clip cages over the following month on young or mature leaves.201121268231
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