Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the persistence of brucella melitensis in experimentally infected ewes through three reproductive cycles. | the authors studied the persistence of infection in 46 ewes experimentally infected with brucella melitensis biovar 3 and monitored through three subsequent reproductive cycles. the entire experimental period lasted for 151 weeks. infection of ewes and elimination of brucella in milk, or its presence in vaginal discharges, persisted throughout the duration of the trial, as demonstrated by recurrent elimination of brucella in milk and vaginal discharges. brucella melitensis was recovered from the ... | 2005 | 16283920 |
| characteristics of b. melitensis versus b. abortus bacteraemias. | to determine the epidemiological and the clinical characteristics of bacteremic brucellosis. | 2005 | 15603839 |
| modulation of phospholipase a2 activity in primary cultures of rat cortical neurons. | in neurons, phospholipase a2 (pla2) plays a central role in the regulation of membrane phospholipid metabolism. we have addressed the pharmacological modulation of pla2 in primary cultures of rat cortical neurons. inhibition curves were obtained in 4 day-in-culture neurons treated for 30 minutes with either the dual pla2 inhibitor methyl arachidonyl fluorophosphonate (mafp), or the ipla2 inhibitor bromoenol lactone (bel). full inhibition was achieved with 100 and 250 microm of mafp, or 10 and 20 ... | 2005 | 15682269 |
| [brucellosis at the dawn of the 21st century]. | human brucellosis is now a rare disease in countries where eradication programs (especially vaccination) against brucellosis in cattle, sheep, and goats have been successfully implemented. in france, fewer than 50 brucellosis cases are annually notified to the national institute for infection surveillance. human brucellosis, however, remains endemic in the mediterranean basin, middle east, western asia, africa, and south america. shortcomings of standard diagnostic methods for brucellosis (varia ... | 2005 | 15695027 |
| indirect enzyme-linked immunosorbent assay for detection of brucella melitensis-specific antibodies in goat milk. | brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. few cases of b. melitensis infection in goats have occurred in the united states over the last 25 years. however, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not introduced into the u.s. goat population. the objective of this study was to develop a sensitive and specific indirect enzyme-linked immunosorbent assay (ielisa) for the detection of ... | 2005 | 15695670 |
| synergy tests by e test and checkerboard methods of antimicrobial combinations against brucella melitensis. | two different synergy testing methods, the checkerboard and the e test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 brucella melitensis strains isolated from blood cultures. the rate of agreement of the e test and checkerboard methods was found to be 55%. the most concordant results were found for the streptomycin-doxycycline combination in 12 (75%) tests, in which four strains showed synergistic activity by e test and antagonistic activi ... | 2005 | 15634962 |
| a case of brucellar septic arthritis of the knee with a prolonged clinical course. | brucellosis is a systemic infectious disease with a broad spectrum of clinical manifestations. arthritis is frequently observed in its course and may be one of the main presenting clinical features of the disease. we report a case of brucellar monoarthritis of the knee with a prolonged clinical course despite efficient antibiotic treatment. | 2005 | 15007623 |
| 18fdg-pet for monitoring disease activity in an hiv-1 positive patient with disseminated chronic osteomyelitic brucellosis due to brucella melitensis. | 2005 | 15549294 | |
| comparison of fluorescence polarization assay with card and complement fixation tests for the diagnosis of goat brucellosis in a high-prevalence area. | an evaluation of fluorescence polarization assay (fpa) to detect antibodies against brucella melitensis according to the mexican official norm (nom) was performed. in this study, a total of 2582 goat serum samples from a high-prevalence area in northeast mexico where vaccination is applied, were used. of these, 1094 were classified as nom negatives (card test (ct) negatives or ct positives/complement fixation test (cft) negatives) and 1488 as nom positives (ct and cft positives). the receiver op ... | 2006 | 16242780 |
| live nativity and brucellosis, sicily. | 2006 | 17354346 | |
| development and validation of a competitive elisa kit for the serological diagnosis of ovine, caprine and bovine brucellosis. | a competitive elisa (brucella-ab c-elisa) was standardized and validated for the detection of brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (mab 4b5a) produced against brucella melitensis biotype 2. the specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (b/bo%). when compared with an indirect elisa, the brucella-ab c-elisa did not demonstrate cross-reactions when testing positive sera for antibodies to some enterobacteriaceae. a compariso ... | 2006 | 17123429 |
| brucellosis with pulmonary involvement in a premature infant. | congenital brucellosis is rare. a premature infant with transplacentally acquired congenital brucellosis and pulmonary involvement is described. | 2006 | 17132304 |
| [have we defeated the principal zoonoses?]. | following an overview of some successful campaigns against zoonoses, this paper examines other zoonotic diseases that are likely to be brought under control in industrialized countries, such as brucellosis, tuberculosis and canine or wildlife rabies. the author goes on to explain the reasons for the failure to eradicate some other zoonoses in developing countries, and concludes by examining reasons for optimism or pessimism, taking into account new methods of prevention and control. | 2006 | 17140096 |
| mariner mutagenesis of brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival. | random gene inactivation used to identify cellular functions associated with virulence and survival of brucella spp has relied heavily upon the use of the transposon tn5 that integrates at g/c base pairs. transposons of the mariner family do not require species-specific host factors for efficient transposition, integrate nonspecifically at t/a base pairs, and, at a minimum, provide an alternative approach for gene discovery. in this study, plasmid vector psc189, containing both the hyperactive t ... | 2006 | 17176467 |
| [brucellosis: clinical presentation, diagnosis, complications and therapeutic options]. | brucellosis is a zoonotic disease that causes systemic symptoms and can involve many organs and tissues. the major sources of infection are consumption of unpasteurized diary products and occupational contact. brucella is a small, gram-negative coccobacillus that grows slowly in vitro. there are four species of brucella that are pathogenic for humans; in israel brucella melitensis is still the most frequent organism. there are several methods to identified the organism and make the diagnosis (1) ... | 2006 | 17183958 |
| infective endocarditis due to brucella. | one of the complications of brucellosis is infective endocarditis, which carries a high mortality rate if undiagnosed or misdiagnosed. we report a case of brucella infective endocarditis, which was diagnosed serologically and by polymerase chain reaction. after brucella specific treatment, patient showed dramatic improvement clinically, as evident by echocardiogram findings and other investigations. | 2006 | 17185849 |
| acute pancreatitis associated with brucellosis. | acute pancreatitis can be caused by a variety of infectious agents but it is regarded as an extremely rare complication of brucellosis. | 2006 | 16407621 |
| isolation of brucella melitensis from a patient with hearing loss. | 2006 | 16418830 | |
| nnra is required for full virulence and regulates several brucella melitensis denitrification genes. | we identified two regulators of denitrification genes in brucella melitensis 16m: narr, which regulates the nitrate reductase (nar) operon, and nnra, which is involved in the expression of the last three reductases of the denitrification pathway (nirk, norb, and nosz). nnra is required for virulence in mice and for intracellular resistance to nitric oxide. | 2006 | 16452445 |
| in vitro susceptibilities of brucella melitensis isolates to eleven antibiotics. | brucellosis is an endemic disease present in many countries worldwide, but it is rare in europe and north america. nevertheless brucella is included in the bacteria potentially used for bioterrorism. the aim of this study was the investigation of the antibiotic susceptibility profile of brucella isolates from areas of the eastern mediterranean where it has been endemic. | 2006 | 17014707 |
| exudative pleural effusion due to brucellosis in a patient with chronic obstructive pulmonary disease. | pleural involvement is a rare presentation of brucellosis. we report a patient referred for the evaluation of fever, right-sided pleural effusion and hilar lymph node enlargement. the pleural fluid revealed exudative characteristics with the predominance of neutrophils. brucella melitensis was isolated from bone marrow, pleural effusion and blood cultures. the diagnosis was also confirmed by demonstration of a high brucella agglutinin titre. the patient was successfully treated with rifampicin a ... | 2006 | 17034714 |
| prevalence of bovine and human brucellosis in western algeria: comparison of screening tests. | a serological study was carried out in tiaret province in western algeria on 1032 cows distributed in 95 flocks to estimate the prevalence of brucella infection and to compare the sensitivity and specificity of a range of agglutination tests. screening tests showed 31.5% of herds positive using the buffered plate antigen test and 26.3% using the rose bengal test compared with 15.7% with the complement fixation test. using the complement fixation test as the gold standard for confirmatory tests, ... | 2006 | 17037229 |
| brucellosis: the first case of king chulalongkorn memorial hospital and review of the literature. | brucellosis remains a major zoonotic disease worldwide. it has never been reported at king chulalongkorn memorial hospital (kcmh). the authors describe the first case of brucellosis in kcmh, and also review all previous reports in thailand. the presented case was a 52-year-old thai man, living in phetchabun province, who was diagnosed with idiopathic pulmonary fibrosis two years prior to admission. he presented with prolonged fever, dry cough, weight loss of eight kg over three months, hepatospl ... | 2006 | 17048447 |
| cloning, expression and characterization of immunogenic aminopeptidase n from brucella melitensis. | a 97-kda purified aminopeptidase n (pepn) of brucella melitensis was previously identified to be immunogenic in humans. the b. melitensis pepn gene was cloned, expressed in escherichia coli and purified by affinity chromatography. the recombinant pepn (rpepn) exhibited the same biochemical properties, specificity and susceptibility to inhibitors as the native pepn. rpepn was evaluated as a diagnostic antigen in an indirect enzyme-linked immunosorbent assay (elisa) using sera from patients with a ... | 2006 | 17064279 |
| rapid diagnosis of brucella epididymo-orchitis by real-time polymerase chain reaction assay in urine samples. | we studied the diagnostic yield of a real-time polymerase chain reaction assay in urine samples for the rapid diagnosis of brucella epididymo-orchitis compared to that of conventional microbiological techniques. | 2006 | 17070314 |
| chemical and biological factors in the control of brucella and brucellosis. | brucellosis is a highly contagious bacterial zoonosis that affects millions of people worldwide. brucella is highly infectious, especially when aerosolized. the infection induces severe protracted diseases, which are both debilitating and incapacitating, hence, brucella melitensis has been considered a potential biological warfare agent. in the battle against brucella, it is crucial to know its chemical-structure and biochemistry-metabolic characteristics. it is well known that brucella, as well ... | 2006 | 17076637 |
| identification of brucella melitensis 16m genes required for bacterial survival in the caprine host. | brucella species are gram-negative bacteria which belong to alpha-proteobacteria family. these organisms are zoonotic pathogens that induce abortion and sterility in domestic mammals and chronic infections in humans known as malta fever. the virulence of brucella is dependent upon its ability to enter and colonize the cells in which it multiplies. the genetic basis of this aspect is poorly understood. signature-tagged mutagenesis (stm) was used to identify potential brucella virulence factors. p ... | 2006 | 17090391 |
| brucellosis in all patients with febrile neutropenia. | 2006 | 16753892 | |
| protean clinical manifestations and diagnostic challenges of human brucellosis in adults: 16 years' experience in an endemic area. | a prospective study was carried out to elucidate the clinical, epidemiological and laboratory features of human brucellosis. a total of 26 948 blood samples (from adults aged 15 years and above) were screened for serological evidence of brucellosis over a period of 16 years. the slide agglutination/rose bengal plate agglutination test gave positive results in 517 patients, of which 509 had detectable titres by the standard tube agglutination test (sat). the diagnosis of brucellosis was documente ... | 2006 | 16772417 |
| characterization of biological activities of brucella melitensis lipopolysaccharide. | biological activities of lipopolysaccharide (lps) from brucella melitensis 16m were characterized in comparison with lps from escherichia coli o55. lps extracted from b. melitensis was smooth type by electrophoretic analysis with silver staining. the endotoxin-specific limulus activity of b. melitensis lps was lower than that of e. coli lps. there was no significant production of tumor necrosis factor-alpha and nitric oxide in raw 264.7 macrophage cells stimulated with b. melitensis lps, althoug ... | 2006 | 16785713 |
| deletion of znua virulence factor attenuates brucella abortus and confers protection against wild-type challenge. | znua is known to be an important factor for survival and normal growth under low zn(2+) concentrations for escherichia coli, haemophilus spp., neisseria gonorrhoeae, and pasteurella multocida. we hypothesized that the znua gene present in brucella melitensis 16 m would be similar to znua in b. abortus and questioned whether it may also be an important factor for growth and virulence of brucella abortus. using the b. melitensis 16 m genome sequence, primers were designed to construct a b. abortus ... | 2006 | 16790759 |
| evaluation of protection afforded by brucella abortus and brucella melitensis unmarked deletion mutants exhibiting different rates of clearance in balb/c mice. | research for novel brucella vaccines has focused upon the development of live vaccine strains, which have proven more efficacious than killed or subunit vaccines. in an effort to develop improved vaccines, signature-tagged mutant banks were screened to identify mutants attenuated for survival. mutants selected from these screens exhibited various degrees of attenuation characterized by the rate of clearance, ranging from a failure to grow in macrophages after 24 h of infection to a failure to pe ... | 2006 | 16790778 |
| intracellular killing of brucella melitensis in human macrophages with microsphere-encapsulated gentamicin. | treatment of human brucellosis demands antibiotic targeting into the mononuclear-phagocytic system. the aim of this work was to prepare and characterize particulate carriers containing gentamicin and to study their interactions with phagocytic cells and bactericidal activity against intracellular brucella melitensis. | 2006 | 16799160 |
| the stringent response mediator rsh is required for brucella melitensis and brucella suis virulence, and for expression of the type iv secretion system virb. | physiological adaptation of intracellular bacteria is critical for timely interaction with eukaryotic host cells. one mechanism of adaptation, the stringent response, is induced by nutrient stress via its effector molecule (p)ppgpp, synthesized by the action of rela/spot homologues. the intracellular pathogen brucella spp., causative agent of brucellosis, possesses a gene homologous to rela/spot, named rsh, encoding a (p)ppgpp synthetase as confirmed by heterologous complementation of a rela mut ... | 2006 | 16803581 |
| evaluation of oxidative stress and inflammation in long term brucella melitensis infection. | the brucella genus is able to cause chronic infection in a wide range of mammals including humans. oxidative events, lipid peroxidation and inflammatory response against brucella infection have not yet been well elucidated in vivo. we have investigated oxidative/antioxidative status and nitric oxide production in plasma, brain, liver and spleen during a 60 day period of b. melitensis infection in a rat model. in addition, inducible nitric oxide synthase (inos), il-10, il-12, ifn-gamma and tnf-al ... | 2006 | 16810561 |
| the brucella abortus cyclic beta-1,2-glucan virulence factor is substituted with o-ester-linked succinyl residues. | brucella periplasmic cyclic beta-1,2-glucan plays an important role during bacterium-host interaction. nuclear magnetic resonance spectrometry analysis, thin-layer chromatography, and deae-sephadex chromatography were used to characterize brucella abortus cyclic glucan. in the present study, we report that a fraction of b. abortus cyclic beta-1,2-glucan is substituted with succinyl residues, which confer anionic character on the cyclic beta-1,2-glucan. the oligosaccharide backbone is substituted ... | 2006 | 16816173 |
| comparative proteome analysis of laboratory grown brucella abortus 2308 and brucella melitensis 16m. | brucella species are pathogenic agents that cause brucellosis, a debilitating zoonotic disease that affects a large variety of domesticated animals and humans. brucella melitensis and brucella abortus are considered major health threats because of their highly infectious nature and worldwide occurrence. the availability of the annotated genomes for these two species has allowed a comparative proteomics study of laboratory grown b. melitensis 16m and b. abortus 2308 by two-dimensional (2-d) gel e ... | 2006 | 16823981 |
| microbial drug efflux proteins of the major facilitator superfamily. | drug efflux proteins are widespread amongst microorganisms, including pathogens. they can contribute to both natural insensitivity to antibiotics and to emerging antibiotic resistance and so are potential targets for the development of new antibacterial drugs. the design of such drugs would be greatly facilitated by knowledge of the structures of these transport proteins, which are poorly understood, because of the difficulties of obtaining crystals of quality. we describe a structural genomics ... | 2006 | 16842212 |
| efficacy of several antibiotic combinations against brucella melitensis rev 1 experimental infection in balb/c mice. | the objective of the present study was to compare the efficacy of gentamicin given alone or combined with doxycycline with that of standard combination therapies in balb/c mice experimentally infected with the brucella melitensis vaccine strain rev 1. | 2006 | 16849379 |
| abducens nerve palsy and optic neuritis as initial manifestation in brucellosis. | cranial nerve involvement in brucellosis is rare. we present a case of brucellosis presenting with optic neuritis and abducens nerve palsy on the left side. cerebrospinal fluid findings indicated brucella meningitis with high protein count, low sugar level and pleocystosis. in addition, brucella agglutination test (wright test) was found to be 1/128 in cerebrospinal fluid. serum agglutination test for brucella was also positive at 1/1280. this case was diagnosed as brucellosis involving optic an ... | 2006 | 16857626 |
| a footprint of brucella infection: enormous saccular aneurysm of the ascending aorta. | 2006 | 16908703 | |
| evaluation of the clinical presentations in neurobrucellosis. | brucellosis is a multisystem disease that may present with a broad spectrum of clinical manifestations and complications. neurobrucellosis is one of the complications. | 2006 | 16914346 |
| brucella melitensis triggers time-dependent modulation of apoptosis and down-regulation of mitochondrion-associated gene expression in mouse macrophages. | brucella spp. are facultative intracellular bacteria that cause brucellosis in humans and other animals. brucella spp. are taken up by macrophages, and the outcome of the macrophage-brucella interaction is a basis for establishment of a chronic brucella infection. microarrays were used to analyze the transcriptional response of the murine macrophage-like j774.a1 cell line to infection with virulent brucella melitensis strain 16m. it was found that most significant changes in macrophage gene tran ... | 2006 | 16926395 |
| identification of protein candidates for developing bacterial ghost vaccines against brucella. | 2006 | 16929687 | |
| an rpoh-like heat shock sigma factor is involved in stress response and virulence in brucella melitensis 16m. | b. melitensis 16m genome analysis revealed the presence of six putative sigma factor-encoding genes: rpod, rpoh1, rpoh2, rpoe1, rpoe2, and rpon. we mutated all these genes except rpod. phenotypic analysis of the mutants reveals that a strain carrying an rpoh2 null mutation (deltarpoh2) is impaired for growth at 21 and 42 degrees c and shows increased sensitivity to hydrogen peroxide. compared to the wild-type strain, the deltarpoh2 mutant is attenuated in all virulence models tested. three other ... | 2006 | 16936018 |
| the hmuq and hmud genes from bradyrhizobium japonicum encode heme-degrading enzymes. | utilization of heme by bacteria as a nutritional iron source involves the transport of exogenous heme, followed by cleavage of the heme macrocycle to release iron. bradyrhizobium japonicum can use heme as an iron source, but no heme-degrading oxygenase has been described. here, bioinformatics analyses of the b. japonicum genome identified two paralogous genes renamed hmuq (bll7075) and hmud (bll7423) that encode proteins with weak similarity to the heme-degrading monooxygenase isdg from staphylo ... | 2006 | 16952937 |
| identification of smooth and rough forms in cultures of brucella melitensis strains by flow cytometry. | brucella melitensis strains may occur as either smooth or rough variants depending on the expression of o-polysaccharides (ops) as a component of the bacterial outer membrane lipopolysaccharide (lps). the wboa gene, which codes for the enzyme glycosyl transferase, is essential for the assembly of o-chain in brucella. deletion of wboa in smooth virulent b. melitensis 16m results in a rough mutant designated wrr51. we developed a flow cytometric method to determine the proportion of b. melitensis ... | 2006 | 16965789 |
| evaluation of immunomagnetic separation-polymerase chain reaction in direct detection of brucella abortus and brucella melitensis from cheese samples. | the current study was carried out to assess the use of immunomagnetic separation-polymerase chain reaction (ims-pcr) in direct detection of brucella abortus and b. melitensis from soft cheese and to examine a relatively small number of field samples for the presence of these species. two methodologies, one with ims and the other without ims, were employed for recovery of the brucella species from cheese samples. ims in conjunction with the pcr assay was determined to detect as low as 3x10(2) bac ... | 2006 | 16972772 |
| comparison of protective efficacy of subcutaneous versus intranasal immunization of mice with a brucella melitensis lipopolysaccharide subunit vaccine. | groups of mice were immunized either subcutaneously or intranasally with purified brucella melitensis lipopolysaccharide (lps) or with lps as a noncovalent complex with neisseria meningitidis group b outer membrane protein (lps-gbomp). control mice were inoculated with sterile saline. two doses of vaccine were given 4 weeks apart. mice were challenged intranasally with virulent b. melitensis strain 16m 4 weeks after the second dose of vaccine. sera, spleens, lungs, and livers of mice were harves ... | 2006 | 16988260 |
| [a case of brucellosis presenting with urinary tract infection]. | in this report an acute brucellosis case presenting with the symptoms of urinary tract infection (uti) has been discussed. a 29 years old male patient was admitted to the emergency service of our hospital with the complaints of acute uti. his complaints were persisting for five days before admission. his physical examination was normal but there were fever (39 degrees c) and costovertebral angle tenderness. his medical history revealed that multiple stones in the lower pole of the right kidney a ... | 2006 | 17001859 |
| fiber-optic microsphere-based arrays for multiplexed biological warfare agent detection. | we report a multiplexed high-density dna array capable of rapid, sensitive, and reliable identification of potential biological warfare agents. an optical fiber bundle containing 6000 individual 3.1-mum-diameter fibers was chemically etched to yield microwells and used as the substrate for the array. eighteen different 50-mer single-stranded dna probes were covalently attached to 3.1-mum microspheres. probe sequences were designed for bacillus anthracis, yersinia pestis, francisella tularensis, ... | 2006 | 16478092 |
| experiments on a sub-unit vaccine encapsulated in microparticles and its efficacy against brucella melitensis in mice. | the aim of this study was to evaluate the effect of the excipients used to facilitate the encapsulation of high hydrophobic antigenic complex extracted from brucella ovis (hs) on the physico-chemical properties of the resulting microparticles. poly(epsilon-caprolactone) (pec) microparticles containing hs were prepared by the solvent extraction/evaporation method using total recirculation one-machine system (troms). different excipients, beta-cyclodextrin (beta-cd), pluronic f68, tween 20 or twee ... | 2006 | 16481077 |
| clinical and laboratory features of brucellosis in two university hospitals in southeast turkey. | this prospective study was carried out in two university hospitals between january 2000 and december 2002. the diagnosis of brucellosis was made with compatible clinical findings, positive brucella agglutination > or =1/160 titres, and/or the isolation of brucella species. the patients were followed up without intervention. one hundred and thirty-eight patients with active brucellosis were evaluated. of the participants, 79 (57.2%) cases were acute, 23 (16.7%) sub-acute and 36 (26.1%) chronic. b ... | 2006 | 16483439 |
| utility of an immunocapture-agglutination test and an enzyme-linked immunosorbent assay test against cytosolic proteins from brucella melitensis b115 in the diagnosis and follow-up of human acute brucellosis. | the utility of an immunocapture-agglutination (brucellacapt, vircell sl, granada, spain) test and an enzyme-linked immunosorbent assay igg, iga, and igm (elisa-igg, elisa-iga, elisa-igm) against cytosolic proteins from brucella melitensis b115 (r) was compared with elisa-igg, elisa-iga, and elisa-igm against smooth lipopolysaccharide (s-lps) from b. melitensis 16m (s), serum agglutination test (sat), and coombs test in the diagnosis and follow-up for 10 months of 51 patients with acute brucellos ... | 2006 | 16500069 |
| [psoas abcess and brucellosis]. | 2006 | 16500734 | |
| brucella outer membrane protein omp31 is a haemin-binding protein. | the expression of haemin-binding proteins (hbps) in the outer membrane is one of the strategies used by gram-negative bacteria to obtain iron from the host. no hbp has been described in brucella spp. we investigated whether omp31, an outer membrane protein from brucella with homology to hbps from bartonella quintana, is an hbp. soluble recombinant omp31 bound specifically to haemin-agarose, while an unrelated brucella protein (sura) did not. a similar experiment showed that native omp31 found in ... | 2006 | 16517201 |
| residual virulence and immunogenicity of cgv26 and cgv2631 b. melitensis rev. 1 deletion mutant strains in sheep after subcutaneous or conjunctival vaccination. | the cgv26 and cgv2631 strains are novel engineered brucella melitensis rev.1 mutant strains deleted for the bp26 gene or for both bp26 and omp31 genes, respectively, coding for proteins of diagnostic significance. the residual virulence and immunogenicity of both mutants were compared to the parental rev.1 strain in sheep after subcutaneous or conjunctival vaccination. the deletion of the bp26 gene or both bp26 and omp31 genes had no significant effect on the intracellular survival of the rev.1 ... | 2006 | 16519974 |
| brucella melitensis: review of the human infection case. | during the last several years, brucellosis has become an important public-health problem on a large territory part of bosnia and herzegovina. the disease belongs to the zoonosis group, and can be caused by several bacterium species from brucella genus. for human and veterinarian medicine, b. abortus, b. melitensis, b. suis and b. canis from brucella genus are important, while other brucella species are found only in animals. the results of laboratory process of isolating brucella melitensis, as ... | 2006 | 16533173 |
| use of real-time quantitative polymerase chain reaction to monitor the evolution of brucella melitensis dna load during therapy and post-therapy follow-up in patients with brucellosis. | we performed quantitative real-time polymerase chain reaction (q-pcr) to monitor the evolution of brucella melitensis dna load from initial diagnosis through post-therapy follow-up in patients with brucellosis. | 2006 | 16586386 |
| multiplex pcr assay for the identification and differentiation of all brucella species and the vaccine strains brucella abortus s19 and rb51 and brucella melitensis rev1. | 2006 | 16595839 | |
| total hip arthroplasty infected by brucella: a report of two cases. | infection caused by brucella following total joint arthroplasty is very rare. we present 2 cases of brucellar infection following total hip arthroplasty (tha). a 66-year-old woman who had prolonged contact with cattle presented with pain at the thigh and groin areas. the patient had undergone an uncemented tha for a hip fracture 3 years previously. cultures of articular liquid were positive for brucella abortus. the implant was loosened and was managed with a 2-stage re-implantation. a 71-year-o ... | 2006 | 16598098 |
| attenuated bioluminescent brucella melitensis mutants gr019 (virb4), gr024 (gale), and gr026 (bmei1090-bmei1091) confer protection in mice. | in vivo bioluminescence imaging is a persuasive approach to investigate a number of issues in microbial pathogenesis. previously, we have applied bioluminescence imaging to gain greater insight into brucella melitensis pathogenesis. endowing brucella with bioluminescence allowed direct visualization of bacterial dissemination, pattern of tissue localization, and the contribution of brucella genes to virulence. in this report, we describe the pathogenicity of three attenuated bioluminescent b. me ... | 2006 | 16622231 |
| a simple and rapid protein array based method for the simultaneous detection of biowarfare agents. | a protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. the chip was developed for the arraytube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich-enzyme-linked immunosorbent assay and signal amplification by streptavidin-poly-horseradish peroxidase. specific immunoassays for staphylococcus enterotoxin b, ricin, venezuelan eq ... | 2006 | 16622830 |
| isolated brucella peritonitis in a capd patient. | peritonitis is the most common complication in patients undergoing continuous ambulatory peritoneal dialysis (capd). brucellosis is a rare cause of bacterial peritonitis. only 1 case was reported of a patient with brucella peritonitis during capd therapy. in that case, capd peritonitis was accompanied by acute brucellosis. we present the case of a patient with isolated brucella peritonitis receiving capd therapy without systemic manifestations of brucellosis who works as a farmer. results of a s ... | 2006 | 16632008 |
| cghscan: finding variable regions using high-density microarray comparative genomic hybridization data. | comparative genomic hybridization can rapidly identify chromosomal regions that vary between organisms and tissues. this technique has been applied to detecting differences between normal and cancerous tissues in eukaryotes as well as genomic variability in microbial strains and species. the density of oligonucleotide probes available on current microarray platforms is particularly well-suited for comparisons of organisms with smaller genomes like bacteria and yeast where an entire genome can be ... | 2006 | 16638145 |
| evaluation of novel brucella melitensis unmarked deletion mutants for safety and efficacy in the goat model of brucellosis. | pregnant goats were employed to assess unmarked deletion mutant vaccine candidates bmdeltaasp24, bmdeltacydba, and bmdeltavirb2, as the target host species naturally infected with brucella melitensis. goats were assessed for the degree of pathology associated with the vaccine strains as well as the protective immunity afforded by each strain against abortion and infection after challenge with wild-type brucella melitensis 16m. both bmdeltaasp24 and bmdeltavirb2 were considered safe vaccine candi ... | 2006 | 16697090 |
| a new variant of brucella melitensis. | brucella melitensis is highly pathogenic and constitutes a serious risk to public health. in argentina, biovar 1 has been isolated from infected animals, but the rev.1 strain vaccine is not authorised for use. this report describes nine atypical b. melitensis isolates obtained from humans. these isolates grew slowly, produced small colonies and were susceptible to penicillin and dyes, similar to the b. melitensis rev.1 vaccine strain, but were inhibited by streptomycin 2.5 mg/l. the isolation of ... | 2006 | 16700714 |
| probable transmission of brucellosis by breast milk. | brucellosis is a zoonosis transmittable to humans. the transmission is mainly by consumption of unpasteurized milk or its products. cases in the first year are very uncommon and other modes of transmission are responsible at this age. we report two children with brucellosis diagnosed at 7 and 2 months old where the probable way of transmission is the breast milk. | 2006 | 16735362 |
| crystal structures of two bacterial 3-hydroxy-3-methylglutaryl-coa lyases suggest a common catalytic mechanism among a family of tim barrel metalloenzymes cleaving carbon-carbon bonds. | the enzyme 3-hydroxy-3-methylglutaryl-coenzyme a (hmg-coa) lyase catalyzes the terminal steps in ketone body generation and leucine degradation. mutations in this enzyme cause a human autosomal recessive disorder called primary metabolic aciduria, which typically kills victims because of an inability to tolerate hypoglycemia. here we present crystal structures of the hmg-coa lyases from bacillus subtilis and brucella melitensis at 2.7 and 2.3 a resolution, respectively. these enzymes share great ... | 2006 | 16330546 |
| brucella outer membrane complex-loaded microparticles as a vaccine against brucella ovis in rams. | due to the important drawbacks of the brucella melitensis rev 1 vaccine, a safer vaccine based on an outer membrane complex from brucella ovis encapsulated in poly-epsilon-caprolactone (pec) microparticles (mp) was developed and tested in rams. homogeneous batches of microparticles were prepared by a new double emulsion solvent evaporation method called "total recirculation one-machine system" (troms). such microparticles presented a mean diameter of 2 microm and displayed an antigen loading of ... | 2006 | 16337315 |
| a complication of brucellosis: epididymoorchitis. | epididymoorchitis is the most frequent genitourinary complication of brucellosis. | 2006 | 16360332 |
| evaluation of the bactec microbial detection system for culturing miscellaneous sterile body fluids. | this study sought to evaluate the efficacy of the bactec blood culture system for culturing sterile body fluids, in comparison with traditional culture methods. a total of 906 specimens were cultured using both conventional media and the bactec detection system. differences in numbers of isolates determined by each system were compared. clinically significant microorganisms were isolated from 15.3% (139) of 906 specimens by both the bactec system and conventional culture, whereas for 8.8% (80) o ... | 2006 | 16364602 |
| foreign-body osteoarticular infection by brucella melitensis: a report of three cases. | 2006 | 16391267 | |
| prevention of laboratory-acquired brucellosis: significant side effects of prophylaxis. | 2006 | 16392095 | |
| a rare case of brucella melitensis infection in an obstetrician during the delivery of a transplacentally infected infant. | we report a case of brucella melitensis infection in an obstetrician who was infected during the delivery of an infant suffering from congenital brucellosis. the obstetrician was treated with doxycycline and rifampin and fully recovered. this is the first reported case of brucella infection transmitted through infectious secretions at the delivery of a transplacentally infected newborn and emphasizes that, especially in endemic areas educational and technical measures are needed in order the obs ... | 2006 | 16249034 |
| an indirect elisa for the detection of antibody in milk from sheep experimentally infected with brucella melitensis biovar 3. | an indirect elisa was evaluated for the detection of brucella antibodies in milk (m-elisa) from sheep experimentally infected with b. melitensis biovar 3. at the end of the second reproductive cycle (13 months post infection), the milk of 22 lactating sheep was tested using the m-elisa. sera from the same sheep were also tested for brucella antibodies using the rose bengal test (rbt) and the complement fixation test (cft). the first serum sampling after parturition showed 100% sensitivity in bot ... | 2006 | 20429056 |
| investigations on brucellosis in egyptian baladi does with emphasis on evaluation of diagnostic techniques. | investigations were carried out on caprine brucellosis in a costal area in egypt. a total number of 577 baladi does was examined for brucella infection using different serological tests. specimens were taken from seropositive obligatory slaughtered does (no = 33) for brucella isolation, histopathological examination, polymerase chain reaction (pcr) assay and determination of serum copper (cu), zinc(zn) and iron (fe) concentrations. results indicated that the incidence of brucellosis was 3.0-5.0% ... | 2007 | 19070039 |
| [response to the treatment of brucellosis among children. evaluation with huddleson reaction and pcr]. | brucellosis poses a significant public health problem and requires meticulous diagnosis; the outcome has frequent relapses even when the treatment is appropriate. | 2007 | 18593545 |
| comparison of pcr assay and bacteriological culture method for the detection of brucella melitensis in stomach content samples of aborted sheep fetuses. | the aim of this study was to evaluate the polymerase chain reaction (pcr) assay for detection of brucella melitensis in stomach content samples of aborted sheep fetuses and to compare its performance with bacteriological culture method. it was also aimed to determine the agreement between pcr and rose bengal plate test (rbpt). materials were collected from aborted sheep from 109 different sheep flocks in the region of van during the lambing seasons of 2004-2005 and 2005-2006. stomach contents fr ... | 2007 | 18181360 |
| the many faces of human-to-human transmission of brucellosis: congenital infection and outbreak of nosocomial disease related to an unrecognized clinical case. | because person-to-person transmission of brucellosis is exceptional, physicians who care for patients with this disease are not considered to be at increased risk. a woman in her 24th week of pregnancy who had received a diagnosis of placenta previa presented to the hospital with massive vaginal bleeding and hypovolemic shock, requiring performance of an emergency cesarean delivery. two physicians who assisted the surgical delivery developed culture-proven brucella melitensis infection. the orga ... | 2007 | 18190307 |
| [evaluation of diagnostic value of brucellacapt test in brucellosis]. | although standard tube agglutination (sta) test is the most preferred method for the serodiagnosis of brucellosis, it may yield false negative results because of its incompetence in detecting the blocking (non-agglutinating) antibodies. blocking antibodies can be detected by coombs' test (anti-human globulin test), which is generally not performed in laboratories routinely since it is a time consuming and laborious method. recently more practical brucellacapt (immunocapture-agglutination) test i ... | 2007 | 18173079 |
| brucella bacteremia in patients with acute leukemia: a case series. | abstract: | 2007 | 18036218 |
| mutant prevention concentrations of tetracycline, rifampicin and ciprofloxacin for brucellae melitensis. | 2007 | 18073161 | |
| [ticks bite in foresters]. | the objective of the study is evalutation of the risk for ticks strings on foresters. the sample constituted by 325 foresters belong to messina province as been submitted to medical examination venous tests. whole sample had to answer to a questionnaire to consider. the prevalence of systemic and skin reactions and we have dose immunoglobulines versus brucella melitensis, rickettsie conorii e borrelia burgdorferi. the results showed that the 19% has declared past stings of tick, and 4.9% reporte ... | 2007 | 18409975 |
| brucellar spondylodiscitis: case report. | brucellosis is a common zoonosis which still remains a major health problem in certain parts of the world. osteoarticular involvement is the most frequent complication of brucellosis, in which the diagnosis of brucellar spondylodiscitis is often difficult since the clinical presentation may be obscured by many other conditions. herein, we report an uncommon case of spondylodiscitis due to brucella in a male who presented with abdominal pain. the diagnosis was established by positron emission tom ... | 2007 | 18229463 |
| brucellar arthritis of the knee: a case report with delayed diagnosis. | brucellar septic arthritis has become extremely rare in western developed countries since bovine brucellosis has been successfully eradicated. nevertheless, we should remain vigilant for this disease, as brucellosis is still endemic in many parts of the world. the authors present the rare manifestation of a brucellar septic arthritis of the knee joint, associated with a lytic lesion of the proximal tibia. the diagnosis of brucellosis was delayed by 11 months because of the microbiological and hi ... | 2007 | 18260497 |
| a brucellosis case presenting with mass formation suggestive for tumor in soft tissue. | we report here a 70-year-old female patient who was diagnosed with brucellosis and presented with mass formation resembling a tumor. the mass was protuberant, 10 cm from the skin surface with a diameter of 15 cm, located at the inferior-lateral region of the left scapula. brucella melitensis was yielded from culture of mass fluid. the patient responded to ceftriaxone, rifampin and doxycycline therapy and recovered without any sequela at the end of surgery and 3 months of medical treatment. | 2007 | 17984747 |
| cervical intramedullary granuloma of brucella: a case report and review of the literature. | the aim of this study was to present a unique case of intramedullary brucellar granuloma (ibg) and to discuss the diagnosis and management. to our knowledge, only one case of thoracic ibg has been reported previously, and our case is the first in cervical spine. a 35-year-old female patient was admitted with headache, pain and weakness in her four extremities. she had no gastrointestinal symptoms and fever. she had been diagnosed with brucella meningitis 3 months ago and a triple therapy of doxy ... | 2007 | 17103231 |
| immunological responses and protective efficacy against brucella melitensis induced by bp26 and omp31 b. melitensis rev.1 deletion mutants in sheep. | the commonly used live attenuated vaccine in ovine brucellosis prophylaxis is brucella melitensis rev.1. this vaccine is known to induce antibody responses in vaccinated animals indistinguishable by the current conventional serological tests from those observed in challenged animals. brucella bp26 and omp31 proteins have shown an interesting potential as diagnostic antigens for ovine brucellosis. accordingly, the bp26 gene and both bp26 and omp31 genes have been deleted from the vaccine strain r ... | 2007 | 17070627 |
| the identification of two protective dna vaccines from a panel of five plasmid constructs encoding brucella melitensis 16m genes. | five candidate genes from the brucella melitensis 16m genome were selected. eukaryotic expression plasmids encoding these antigens were constructed and expression was verified in vitro from transfected cos7 cells. each vaccine was assessed for protective efficacy in a balb/c mouse brucellosis infection model. from these experiments two protective dna vaccines were identified: p-omp25 and p-ialb. the omp25 antigen (bmei1249) has previously been studied in terms of brucella virulence, serodiagnosi ... | 2007 | 17049676 |
| assessment of genetic stability of brucella melitensis rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis. | the assessment of the genetic stability is one of the essential elements to guarantee the biological quality of live anti-bacteria vaccines. live attenuated brucella melitensis rev 1 is the most effective vaccine against brucellosis in small ruminants. thirty-six b. melitensis rev 1 vaccine strains isolated from human or animal sources from different geographic regions, from different commercial batches or laboratory collections were typed by the multiple-locus variable-number tandem repeat (vnt ... | 2007 | 17050051 |
| ftcr is a new master regulator of the flagellar system of brucella melitensis 16m with homologs in rhizobiaceae. | the flagellar regulon of brucella melitensis 16m contains 31 genes clustered in three loci on the small chromosome. these genes encode a polar sheathed flagellum that is transiently expressed during vegetative growth and required for persistent infection in a mouse model. by following the expression of three flagellar genes (flif, flge, and flic, corresponding to the ms ring, hook, and filament monomer, respectively), we identified a new regulator gene, ftcr (flagellar two-component regulator). ... | 2007 | 17056750 |
| induction of immune response in mice with a dna vaccine encoding outer membrane protein (omp31) of brucella melitensis 16m. | brucellosis causes serious economic losses to goat farmers by way of reproductive losses in the form of abortions and stillbirths. nucleic acid vaccines provide an exciting approach for antigen presentation to the immune system. in this study, we evaluated the ability of dna vaccine encoding the omp31 protein of brucella melitensis 16m to induce cellular and humoral immune responses in mice. we constructed eukaryotic expression vectors called ptargetomp31, encoding outer membrane protein (omp31) ... | 2007 | 17014873 |
| elevated camp levels reverse brucella melitensis-induced lipid peroxidation and stimulate il-10 transcription in rats. | brucella species are able to survive and replicate within the phagocytic vacuole of macrophages that induce chronic infection in humans and domestic animals. the activation of oxidative bactericidal activity is one of the defense systems which protect the host from the toxic effects of pathogens. the aim of this study was to evaluate lipid peroxidation, no production, antioxidative system and inflammation during a period of brucella infection in a rat model; in addition to investigate the role o ... | 2007 | 17014875 |
| brucellosis of the sternum and olecranon in an adolescent. | 2007 | 17195720 | |
| clinical findings, diagnostic approach, and outcome of brucella melitensis epididymo-orchitis. | we have studied 912 patients with brucellosis. of these, 631 (69.2%) were male and 48 had epididymo-orchitis, giving an incidence of epididymo-orchitis of 7.6%. the duration of symptoms before diagnosis was 52.5 +/- 70 days. all the patients had fever, swelling, and scrotal pain, but only 2 (4.2%) reported urinary symptoms. seven patients (14.5%) had leukocyte figures above 11 x 10(9)/l, and urine analysis was normal in 69% of the patients. blood cultures were positive in 65.8% of cases. a total ... | 2007 | 17141451 |
| differential expression of homing receptors and vascular addressins in tonsils and draining lymph nodes: effect of brucella infection in sheep. | the differential expression of homing receptors (hr) and complementary vascular addressins was studied in t and b lymphocytes from ovine tonsils and draining lymph nodes (ln) in uninfected and brucella melitensis-infected sheep. in uninfected sheep, cd4+cd25+ t cells expressed proportionally more l-selectin and beta1 integrin than beta7 integrin in pharyngeal and palatine tonsils and in parotid ln (pln), retropharyngeal ln (rln) and the peripheral prescapular ln (psln). in contrast, memory cd4+c ... | 2007 | 17161868 |
| myd88-dependent activation of b220-cd11b+ly-6c+ dendritic cells during brucella melitensis infection. | ifn-gamma is a key cytokine controlling brucella infection. one of its major function is the stimulation of brucella-killing effector mechanisms, such as inducible no synthase (inos)/nos2 activity, in phagocytic cells. in this study, an attempt to identify the main cellular components of the immune response induced by brucella melitensis in vivo is made. ifn-gamma and inos protein were analyzed intracellularly using flow cytometry in chronically infected mice. although tcrbeta(+)cd4(+) cells wer ... | 2007 | 17404301 |
| [a case of brucellosis presenting as high titer negative result by standard tube agglutination test]. | since prozone is a well known phenomenon in the serologic diagnosis of brucella infections, it is necessary to prepare higher serum dilutions in the standard tube agglutination (sta) test for the brucellosis suspected patients. however, due to limited economical support, the serum dilutions generally last at 1/320-1/640 titers in some laboratories in turkey. in this report, a brucellosis case whose sta test was found negative until the titer of 1/1280, has been presented. a 36-year-old female wa ... | 2007 | 17427566 |
| improved immunogenicity of a vaccination regimen combining a dna vaccine encoding brucella melitensis outer membrane protein 31 (omp31) and recombinant omp31 boosting. | in the present study, we report an attempt to improve the immunogenicity of the omp31 antigen by a dna prime-protein boost immunization regimen. we immunized balb/c mice with an omp31 dna vaccine (pciomp31) followed by boosting with recombinant omp31 (romp31) in incomplete freund's adjuvant and characterized the resulting immune responses and the protective efficacy against brucella ovis and b. melitensis infection. immunoglobulin g1 (igg1) and igg2a titers were higher in sera from pciomp31/romp ... | 2007 | 17428946 |