Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
the first report of ovine cerebral neosporosis and evaluation of neospora caninum prevalence in sheep in new south wales.presence of neospora caninum dna was detected in the brain and spinal cord of an adult merino sheep suspected of dying with acute non-suppurative meningoencephalitis and mild to moderate non-suppurative myelitis. the most severe neurological lesions were found in the midbrain at the rostral coliculi with moderate to severe multifocal vasculitis and gliosis. as this was the first known occurrence of cerebral disease in sheep in australia caused by n. caninum, we surveyed sera from five sheep prop ...201020189309
neospora spp. and toxoplasma gondii antibodies in horses in the czech republic.during january 2007, blood samples were collected from 552 healthy horses from nine different regions of the czech republic. sera were tested for serum antibodies to neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. the same samples were tested for serum antibodies against toxoplasma gondii by a latex agglutination test. in total, 131 of 552 (24%) horses reacted positively for neospora antibodies in competitive- ...201020532561
neospora caninum: in vitro culture of tachyzoites in mcf-7 human breast carcinoma cells.the development of neospora caninum tachyzoites, an apicomplexan protozoan parasite, was studied in vitro using the human breast carcinoma cell 7 (mcf-7) as the host cell line. the extracellular nc-1 tachyzoites in mcf-7 cells were observed and counted daily for 6 consecutive days post-infection to establish the growth curve. the intracellular parasites were observed by acridine orange staining using laser scanning confocal microscope. the results indicated that nc-1 tachyzoites invaded mcf-7 ce ...201020566363
neospora caninum: cloning and expression of a gene coding for cytokine-inducing profilin.profilins are actin-binding proteins that in toxoplasma gondii stimulate innate immunity in mice by binding toll-like receptors (tlr) on dendritic cells (dc) leading to release of inflammatory cytokines, primarily il-12 and ifn-gamma. the purpose of the present study was to characterize neospora caninum profilin, termed ncprofilin. recombinant ncprofilin was purified by affinity chromatography, and used to prepare specific antisera to allow characterization of native ncprofilin antigen in n. can ...201020211619
seroprevalence of neospora caninum in gray wolves in scandinavia.transmission of the protozoan parasite neospora caninum between wild and domestic animals has gained some interest during recent years. because of the close relationship between gray wolf (canis lupus) and dog it has been suggested that gray wolf is a definitive host for the parasite. the aim of this study was to estimate the seroprevalence of n. caninum in scandinavian gray wolves and to investigate any geographical patterns of the infection. the investigation was based on blood samples collect ...201020609524
prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies in swine from northeastern brazil.a serologic survey was conducted among 130 swine slaughtered in the public slaughterhouse of the city of patos, paraíba state, northeastern brazil, to determine the prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies, and to verify possible associations between sex of the animals and antibody prevalence. the sera were analyzed by indirect antibody tests, considering 1:64 (t. gondii) and 1:50 (n. caninum) dilutions as cut-off points. the prevalence of anti-t. gondii antibodi ...201020624342
association between seropositivity for neospora caninum and reproductive performance of beef heifers in the pantanal of mato grosso do sul, brazil.neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, ruminants, and horses. the purpose of this study was to evaluate the association between the presence of antibodies to n. caninum and reproductive loss in beef heifers in the south pantanal region of mato grosso, brazil. a total of 1098 heifers were evaluated from breeding to calving with regard to reproductive performance, and serology for neosporosis was assayed by indirect immunofluorescence react ...201020624350
identification of neospora caninum proteins regulated during the differentiation process from tachyzoite to bradyzoite stage by dige.identification of differentially expressed proteins during neospora caninum tachyzoite-bradyzoite conversion processes may lead to a better knowledge of the pathogenic mechanisms developed by this important parasite of cattle. in the present work, a differential expression proteomic study of tachyzoite and bradyzoite stages was accomplished for the first time by applying dige technology coupled with ms analysis. up to 72 differentially expressed spots were visualized (1.5-fold in relative abunda ...201020162558
development of an indirect elisa test using an affinity purified surface antigen (p38) for sero-diagnosis of canine neospora caninum infection.neospora caninum is an intracellular protozoan that infects domestic and wild canids, as well as many warm blooded animals as shown by the isolation of viable parasites and the detection of parasite dna in naturally infected hosts. n. caninum is described as a cause of neuromuscular disease and death in dogs. the dog is also known as a definitive host, shedding oocysts involved in the transmission of the infection to intermediate hosts. this study was conducted to develop an indirect elisa test ...201020434268
molecular characterization of neospora caninum mag1, a dense granule protein secreted into the parasitophorous vacuole, and associated with the cyst wall and the cyst matrix.summary: in neospora caninum and toxoplasma gondii, the parasitophorous vacuole (pv) is synthesized at the time of infection. during tachyzoite-to-bradyzoite stage conversion, the pv is later transformed into a tissue cyst that allows parasites to survive in their host for extended periods of time. we report on the characterization of ncmag1, the n. caninum orthologue of t. gondii mag1 (matrix antigen 1; tgmag1). the 456 amino acid predicted ncmag1 protein is 54% identical to tgmag1. by immunobl ...201020444303
occurrence of antibodies anti-neospora caninum, anti-toxoplasma gondii, and anti-leishmania chagasi in serum of dogs from pará state, amazon, brazil.a cross-sectional study was conducted to determine the occurrence of anti-toxoplasma gondii, anti-neospora caninum, and anti- leishmania chagasi antibodies in dogs of the state of pará, brazil. for this purpose, 129 blood samples were collected from dogs of different ages and gender. samples of 72 dogs were collected from 39 rural properties from 19 municipalities, and 57 samples were from stray dogs, collected after captivity by the center of zoonosis control from the municipality of santarém. ...201020445991
detection of anti-toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by indirect fluorescence assay (ifa) and indirect elisa.the indirect fluorescence assay (ifa) and the indirect elisa were comparatively used to detect igg and igm antibodies for toxoplasma gondii in experimentally and naturally infected primates. in the experimentally infected group, antibodies of diagnostic value were detected at day 9 post-infection (pi) with the ifa (igg and igm) and with igg-elisa. igm-elisa detected antibodies for t. gondii starting at day 3 pi until the end of the experiment (102 days pi). of the 209 naturally infected sera tes ...201020385056
meningoencephalitis associated with sarcocystis spp. in a free-living japanese raccoon dog (nyctereutes procyonoides viverrinus).a free-living, young adult, male japanese raccoon dog (nyctereutes procyonoides viverrinus) was rescued in gifu, japan in march 2009. the animal was weak and emaciated with neurological signs that included head tilt, tremor and tic. the brain showed no gross abnormality at necropsy, but microscopically there was severe meningoencephalitis associated with protozoa, which were morphologically consistent with the asexual developmental stage of sarcocystis spp. the protozoa were immunohistochemicall ...201020163802
conventional and molecular diagnostic testing for the acute neurologic patient.the aim of this review is to describe and evaluate both conventional and molecular diagnostic testing utilized in dogs and cats with acute neurologic diseases. various types of polymerase chain reaction (pcr) are explored along with novel molecular diagnostic testing that ultimately may prove useful in the critical care setting.201020230434
pathogenic characterization in mice of neospora caninum isolates obtained from asymptomatic calves.in this study, we characterized 8 new isolates obtained from healthy but congenitally infected calves using a balb/c mouse model. neospora caninum-infected mice survived without exhibiting any clinical signs of disease. nevertheless, differences among isolates in parasite organ distribution, parasite burden and the severity of histopathological lesions were determined. mice infected with the nc-spain 5h, nc-spain 7 and nc-spain 9 isolates showed higher parasite burdens and more severe brain lesi ...201020233488
necrotizing cerebellitis and cerebellar atrophy caused by neospora caninum infection: magnetic resonance imaging and clinicopathologic findings in seven dogs.adult dogs with neosporosis can develop a variety of neurologic signs. no area of predilection within the nervous system so far has been identified in adult dogs.201020337912
neospora caninum and toxoplasma gondii antibodies in european brown hares in the czech republic, slovakia and austria.european brown hares (lepus europaeus) coming from the czech republic, slovakia and austria were tested for serum antibodies against neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and for serum antibodies against toxoplasma gondii by an indirect fluorescent antibody test. in a total of 925 samples, 280 (30%) and 132 (14%) reacted positively for n. caninum and t. gondii antibodies, respectively. out of 333 hares in the czech republic, 129 (39%) and 71 (21%) reacted ...201020347525
a survey of neospora caninum-associated bovine abortion in large dairy farms of mashhad, iran.neospora caninum is an apicomplexan protozoon causing abortion in cattle worldwide. the present study was designed to assess the importance of this parasite for causing abortion in dairy farms in the mashhad area of iran. of the aborted bovine fetuses, 151 were collected from dairy farms between 2006 and 2008. first, brain samples were examined by polymerase chain reaction (pcr) for the presence of n. caninum dna, diagnosis was complemented with immunohistochemistry (ihc) and fetal serology (eli ...201020352453
a4d12 monoclonal antibody recognizes a new linear epitope from sag2a toxoplasma gondii tachyzoites, identified by phage display bioselection.toxoplasma gondii surface is coated by closely related antigens that belong to srs (sag-1 related sequences) superfamily. two tachyzoite-specific srs antigens, sag1 and sag2, are immunodominant proteins that apparently modulate the virulence of infection by inducing the host immune response against tachyzoites during the acute phase. in this study, we described a conformationally insensitive monoclonal antibody (a4d12mab) that recognizes a linear epitope shared by two isoforms of p22 that is exp ...201019261354
seroprevalence of toxoplasma gondii and neospora caninum in wild and domestic ruminants sharing pastures in galicia (northwest spain).the prevalence of antibodies to the protozoan parasites toxoplasma gondii and neospora caninum were investigated by the direct agglutination test (dat) and celisa, respectively, in 160 roe deer (capreolus capreolus), 177 sheep and 178 cattle sharing pastures in galicia (northwest spain). the seroprevalence for t. gondii was 13.7% in roe deer, 57% in sheep and 7.3% in cattle. the seroprevalence for n. canimum was 6.8%, 10.1% and 24.1% in roe deer, sheep and cattle, respectively. statistically sig ...201019482324
risk factors associated with neospora caninum seropositivity in sheep from the state of alagoas, in the northeast region of brazil.the aim of this study was to identify risk factors associated with neospora caninum seropositivity in sheep from the state of alagoas, in the northeast region of brazil. twenty-six herds were selected, and blood samples were collected from 343 animals > 6-mo-old. the indirect fluorescent antibody test (ifat) was used for serological diagnosis of n. caninum infection. epidemiological questionnaires were applied for each herd to identify the risk factors associated with infection. thirty-three (9. ...201019799489
neuritis of the cauda equina in a dog.this study presents the first case report of neuritis of the cauda equina in a dog, including characterisation of the inflammatory infiltrate. the dog in question, a 6-year-old welsh springer spaniel, was presented with flaccid tail and faecal and urinary incontinence. the histological lesions included severe mononuclear cell infiltration of the nerve roots of the cauda equina and of the lumbar nerve roots. the infiltrate was composed of large numbers of t-lymphocytes and b-lymphocytes and small ...201020840297
microarray analyses of mouse responses to infection by neospora caninum identifies disease associated cellular pathways in the host response.neospora caninum is a coccidian cyst-forming parasite found in a wide range of host species such as mice, dogs and cattle. the development of methods such as vaccines to prevent abortion and fetal loss due to neosporosis would be greatly assisted by further knowledge on immunity and host responses to infection. in this study we used microarray technology to investigate the protective host responses occurring at 6h post infection in the spleen of mice infected with a prototype live n. caninum vac ...201020817048
association between neospora caninum seropositivity and perinatal mortality in dairy heifers at first calving.associations between neospora caninum infection and the reproductive performance of dairy heifers in their first and second pregnancy on 18 uk farms were examined. six-month-old heifer calves were tested for n caninum-specific antibodies with a commercial elisa, and were then monitored until their second calving. random-effects regression analyses were performed on the data, using serological status as the explanatory variable. of 460 heifers tested, 7.2 per cent were seropositive. seropositive ...201020643884
evaluation of a commercial elisa for the specific detection of antibodies against besnoitia besnoiti.bovine besnoitiosis is an economically important disease in cattle caused by the protozoan parasite besnoitia besnoiti, which occurs endemically in many countries of africa and asia and is spreading in europe. serological identification of subclinically infected cattle is important to avoid the introduction of infected animals into naive herds. here we determine the sensitivity and specificity of the priocheck(®) besnoitia ab, a serological test recently introduced into the european market. anal ...201021035269
neospora caninum: evaluation of vertical transmission in slaughtered beef cows (bos indicus).neospora caninum is a protozoan parasite that causes the most important reproductive problems in cattle worldwide. the objective of this study was to evaluate the possibility of vertical transmission of n. caninum in zebus breed beef cows (bos indicus) submitted for slaughter at an abattoir in the northern region of the state of paraná, southern brazil. one hundred and fifty-nine cows were evaluated: 83 pregnant (in different stages of gestation) and 76 non-pregnant. serum determination of n. ca ...201021063729
influence of adjuvant and antigen dose on protection induced by an inactivated whole vaccine against neospora caninum infection in mice.in this study, the protection afforded by a neospora caninum inactivated vaccine formulated with three different adjuvants (water-in-oil emulsion, aluminum hydroxide with cpg oligodeoxynucleotides and aluminum hydroxide with ginseng extract) and three different parasite doses (10(5), 5 × 10(5) or 10(6) inactivated whole tachyzoites) was evaluated using a mouse model. mice were immunized subcutaneously twice at three-week intervals with inactivated nc-spain 1h tachyzoites and challenged by intrap ...201021067865
detection of neospora caninum tachyzoites in cerebrospinal fluid of a dog following prednisone and cyclosporine therapy.a 9-year-old female spayed shetland sheepdog was presented to the kansas state university veterinary medical teaching hospital for evaluation following a 3-week history of left rear limb lameness that had progressed to generalized ataxia. multifocal or diffuse brain lesions were suspected based on physical examination findings. cerebrospinal fluid (csf) contained 52 nucleated cells/μl composed of mixed inflammatory cells. treatment with prednisone and cyclosporine was initiated based on a presum ...201020698940
immune response to neospora caninum native antigens formulated with immune stimulating complexes in calves.the aim of this study was to compare the immune responses to live neospora caninum tachyzoites and n. caninum native antigens formulated with immune stimulating complexes matrix (iscom-matrix) in calves. fifteen calves were used in this study: 3 were intravenously inoculated with 1 × 10(8) live tachyzoites (group a), 3 were inoculated twice with n. caninum native antigens formulated with iscoms (group b); 3 with n. caninum native antigens in phosphate-buffered saline (pbs) (group c); 3 received ...201021074325
monensin use against neospora caninum challenge in dairy cattle.using a randomized controlled trial design, a randomly allocated intervention group of 15 cows received a slow-release bolus that delivered 100 days of monensin. the negative control group of 15 cows received a placebo bolus that was identical to the monensin bolus, except without the monensin. two weeks after bolus administration, all cows were challenged with a 2 ml subcutaneous injection of a live tachyzoite suspension. whole blood and serum samples were collected from each cow every week for ...201021106293
detection of toxoplasma gondii, neospora caninum, and encephalitozoon cuniculi in the brains of common voles (microtus arvalis) and water voles (arvicola terrestris) by gene amplification techniques in western austria (vorarlberg).knowledge about the protozoan parasite fauna in voles (arvicolinae) in austria is rather limited, although some of these pathogens play an important role in human medicine and cause zoonoses (e.g., toxoplasma gondii and encephalitozoon cuniculi). others are of relevance in veterinary medicine and have a negative economic impact (e.g., neospora caninum). two hundred sixty-eight common voles (microtus arvalis) and 86 water voles (arvicola terrestris) from the most western austrian province, vorarl ...201020480373
prevalence of toxoplasma gondii and neospora caninum antibodies in spanish ibex (capra pyrenaica hispanica).a cross-sectional study was carried out on spanish ibex populations in southern spain to assess the seroprevalence of toxoplasma gondii and neospora caninum and to investigate the risk factors associated with these infections. using the modified agglutination test, the seroprevalence to t. gondii was 27.5% (146/531; ci(95%), 23.7-31.3), and this seropositivity significantly increased with age. among adults, statistically significant differences were observed between geographical locations and ov ...201021159529
evidence of congenital transmission of neospora caninum in naturally infected water buffalo (bubalus bubalis) fetus from brazil.the aim of this study was to determine the congenital infection by neospora caninum in the water buffalo (bubalus bubalis), a natural intermediate host. nine pregnant water buffalos, raised under free-grazing condition, were slaughtered, and their fetuses were collected. samples of brain and thoracic fluid were obtained from those fetuses, with gestational ages ranging from 2 to 5 months. the dna of n. caninum was detected and identified in the brain of one of those fetuses, using two pcr assays ...201021181191
prevalence of antibodies to trypanosoma cruzi, toxoplasma gondii, encephalitozoon cuniculi, sarcocystis neurona, besnoitia darlingi, and neospora caninum in north american opossums, didelphis virginiana, from southern louisiana.we examined the prevalence of antibodies to zoonotic protozoan parasites ( trypanosoma cruzi, toxoplasma gondii, and encephalitozoon cuniculi) and protozoans of veterinary importance ( neospora caninum, sarcocystis neurona, and besnoitia darlingi) in a population of north american opossums ( didelphis virginiana) from louisiana. samples from 30 opossums were collected as part of a survey for t. cruzi in louisiana. frozen sera from these 30 opossums were examined using an indirect immunofluoresce ...201021158620
neospora caninum and toxoplasma gondii antibody prevalence in alaska wildlife.free-ranging caribou and moose populations in some regions of alaska undergo periodic declines in numbers. caribou and moose are managed by the state as valuable resources for not only sustenance and subsistence, but also for cultural heritage. incidence and prevalence of diseases that may impact herd health and recruitment from year to year are relevant to management decisions aimed to protect the long-term viability of these herds. neospora caninum and toxoplasma gondii are two apicomplexan pa ...201020688628
prevalence of antibodies against toxoplasma gondii and neospora caninum in moose (alces alces) and roe deer (capreolus capreolus) in sweden.toxoplasma gondii and neospora caninum are two coccidian parasites with a worldwide distribution. t. gondii is one of the more common parasitic zoonoses in the world and in young children and immunocompromised persons, infection can lead to severe disease and death. n. caninum is an important cause of abortions in cattle. wildlife have been identified as reservoirs and transmitters for both parasites. the purpose of this study was to investigate the seroprevalences of t. gondii, and n. caninum i ...201021232869
evaluation of toxoplasma gondii and neospora caninum infections in sheep from uberlândia, minas gerais state, brazil, by different serological methods.toxoplasmosis and neosporosis have been recognized as economically important diseases with considerable impact on the livestock industry. considering the scarce information on the occurrence of toxoplasma gondii and neospora caninum infections in sheep from uberlândia, minas gerais state, brazil, this study aimed to investigate the frequency of antibodies against these parasites in sheep sera from this region by using different serological methods. a total of 155 sheep serum samples were analyze ...201021075529
prevalence of antibodies against neospora caninum in dogs from urban areas in central poland.neospora caninum is a protozoan parasite which causes abortion in cattle as well as reproduction problems and neurological disorders in dogs. to assess the prevalence of the parasite in urban dogs in the mazovian voivodeship, central poland, serum samples from 257 dogs were analyzed for the presence of specific igg antibodies. the examined dogs visited three private veterinary clinics located in warsaw due to control tests, vaccinations, or other reasons not directly connected with neosporosis. ...201021079995
serological profile of toxoplasma gondii and neospora caninum infection in commercial sheep from são paulo state, brazil.neosporosis and toxoplasmosis are two important infections in young and adult sheep, leading to low production and abortion. this study aimed to determine the frequency of antibodies to toxoplasma gondii and neospora caninum in sheep from the eastern region of são paulo state, brazil. serum samples (382) were collected from the sheep and assayed for t. gondii through modified agglutination test (mat) and indirect fluorescent antibody test (ifat), and for n. caninum antibodies, through ifat, with ...201021256676
detection of specific antibodies anti-neospora caninum in the fallow deer (dama dama).sera from 335 farmed fallow deer (dama dama) at the breeding station in kosewo górne in the mazurian lake district, north-east poland, were investigated for the presence of antibodies against neospora caninum. the distribution of age groups was as follow: >4years - 154 animals; 2years - 76 animals; 1year - 105 animals. ten sera with the optical density exceeding 0.159 absorbance units (i.e., cut-off value) in elisa test were also analyzed by western blot. western blot analysis revealed seroreact ...201021087781
neospora caninum infection in stray and farm dogs: seroepidemiological study and oocyst shedding.in a previous study, farm and stray dogs were considered potential high risk populations of neospora caninum infection in spain. consequently, we decided to investigate the significance of n. caninum infection in these populations. specific antibodies were detected in 120 out of 275 dog sera (43.6%), with titres ranging from 1:50 to 1:800. differences in seroprevalence between farm (47.5%, 67/141) and stray (39.5%, 53/134) dogs were not significant (p>0.05; χ(2) test), but farm dogs showed signi ...201020851523
Occurrence of antibodies against Neospora caninum and/or Toxoplasma gondii in dogs with neurological signs.This study aimed to evaluate occurrences of antibodies against Neospora caninum and Toxoplasma gondii in dogs with neurological signs. Blood samples from 147 dogs were collected: 127 from owned dogs (attended at the Veterinary Teaching Hospital of the Federal University of Paraná (HV-UFPR) and at private veterinary clinics in the city of Curitiba), and 20 from stray dogs found in Curitiba's metropolitan region. The dogs presented one or more of the following neurological signs: seizures, paresis ...201121961748
vaccines against a major cause of abortion in cattle, neospora caninum infection.neosporosis, caused by the apicomplexan parasite neospora caninum, represents one of the economically most important causes of abortion in cattle. during pregnancy, the parasite infects the placental tissue and the fetus, which can lead to stillbirth, abortion, or birth of weak calves. alternatively, calves are born without clinical symptoms, but they can carry over the parasite to the next generation. in addition, n. caninum causes neuromuscular disease in dogs. the economic importance of neosp ...201126486502
oocysts and high seroprevalence of neospora caninum in dogs living in remote aboriginal communities and wild dogs in australia.canines are definitive hosts of neospora caninum (apicomplexa). for horizontal transmission from canines to occur, viable oocysts of n. caninum must occur in the environment of susceptible intermediate hosts. canids in australia include wild dogs and aboriginal community dogs. wild dogs are those dogs that are not dependent on humans for survival and consist of the dingo, feral domestic dog and their hybrid genotypes. aboriginal community dogs are dependent on humans, domesticated and owned by a ...201122245069
neospora caninum is associated with abortion in algerian cattle.abstract neospora caninum is a major cause of abortion in cattle worldwide. however, little information is available for algeria. accordingly, 799 cattle from 87 farms in the north and northeast of algeria were enrolled in a seroepidemiological survey. an indirect fluorescence antibody test (ifat) revealed a seroprevalence of 19.6%. the animals were divided into 3 groups according to their breed: imported european cattle, local breeds, and crossed animals (european x local). seroprevalences were ...201121728878
clinicopathologic diagnosis of cutaneous toxoplasmosis in an angora cat.this report deals with a case of cutaneous toxoplasmosis in a 2 year-old female angora cat. cutaneous lesions were characterized by prescapular ulcers and hyperemic nodules in the skin of the inguinal and dorsosacral regions. a skin biopsy sample was collected from the lesioned area and processed for histopathologic examination and immunoperoxidase test using toxoplasma gondii and neospora caninum specific antibodies. toxoplasma gondii immunopositive reactions were detected in keratinocytes and ...201121950216
the snsag merozoite surface antigens of sarcocystis neurona are expressed differentially during the bradyzoite and sporozoite life cycle stages.sarcocystis neurona is a two-host coccidian parasite whose complex life cycle progresses through multiple developmental stages differing at morphological and molecular levels. the s. neurona merozoite surface is covered by multiple, related glycosylphosphatidylinositol-linked proteins, which are orthologous to the surface antigen (sag)/sag1-related sequence (srs) gene family of toxoplasma gondii. expression of the sag/srs proteins in t. gondii and another related parasite neospora caninum is lif ...201121775062
seroprevalence of neospora spp. in horses in south of iran.neospora caninum, an apicomplexan protozoan parasite, is recognized as a major cause of abortion in cattle. however, limited information is presently available on the seroprevalence of neospora antibodies in horses worldwide. the aim of the present study is to determine serological prevalence of neospora infection in horses in iran. blood samples were obtained from 200 horses and tested for serum antibodies against neospora spp. by the neospora modified direct agglutination test (n-mat). antibod ...201122433879
seroprevalence of neospora caninum infection in dairy cattle in tabriz, northwest iran.the aim of this study was to determine the seroprevalence of antibody to neospora caninum in healthy and aborted dairy cattle in tabriz, capital of east-azarbaijan in northwest of iran.201122347319
seroprevalence of neospora spp. in horses in north east of iran.neospora caninum, an obligate intracellular protozoan parasite, is recognized as a major cause of abortion in cattle, while limited information is presently available on the seroprevalence of neospora antibodies in horses' worldwide. the aim of the present study was to determine serologic prevalence of neospora infection in horses in iran.201122347290
neospora caninum and leishmania infantum co-infection in domestic dogs (canis familiaris) in meshkin-shahr district, northwestern iran.mediterranean visceral leishmaniasis (mvl) is an infectious disease that affects both human and animals. domestic dogs (canis familiaris) are principal reservoir hosts of mvl caused by leishmania infantum. dogs are definitive hosts for neospora caninum and a risk factor for infecting intermediate hosts. the immunosuppression caused by visceral leishmaniasis (vl) can promote the occurrence of co-infections with other agents such as neosporosis. this study aimed to determine the frequency of co-in ...201122808419
neosporosis in a captive parma wallaby (macropus parma).infection with neospora caninum has been diagnosed in a variety of animal species; however, reports in marsupials are rare. a captive parma wallaby (macropus parma) died suddenly and was subjected to necropsy examination. the main finding was necrotizing myocarditis associated with protozoan parasites. the protozoa were identified as n. caninum by use of immunohistochemistry and partial gene sequence analysis. neospora and toxoplasma should be considered a possible cause of disease in captive ma ...201121689827
Identification of a gene cluster for cell-surface genes of the SRS superfamily in Neospora caninum and characterization of the novel SRS9 gene.SUMMARYHere we present the detection of a gene cluster for Neospora caninum surface genes, similar to the Toxoplasma gondii SRS9 locus, and the cloning and characterization of the NcSRS9 gene. PCR genome walking, using NcBSR4 gene as a framework, allows the identification, upstream NcBSR4, of 2 sequences homologous to the SRS5 and the Ubiquinol-cytochrome C reductase genes and, downstream NcBSR4, of an ORF of 1191 bp coding for a 396-amino acid polypeptide with 59% similarity to the TgSRS9 antig ...201121939586
sero-prevalence of neospora caninum and besnoitia besnoiti in south australian beef and dairy cattle.a serological survey for neospora caninum and besnoitia besnoiti was carried out in beef and dairy cattle in south australia. serum samples of dairy cattle (n=133) from 9 properties and tank milk samples from a further 122 dairy herds were tested. an additional 810 sera from beef cattle from 51 properties were also tested. testing at the individual animal level by idexx neospora x2 ab test elisa revealed a low prevalence of n. caninum antibodies of only 2.7% (95% ci; 1.6-3.7%) sera positive, as ...201122138306
Potential involvement of Neospora caninum in naturally occurring ovine abortions in New Zealand.Neospora caninum is an obligate intracellular parasite and is recognised as the leading cause of bovine abortion worldwide. Natural infection with N. caninum has been described in sheep but it has generally not been regarded as a significant cause of abortion. Recently, there have been several New Zealand cases of foetal abortions where N. caninum was detected which strongly suggested the involvement of Neospora in these abortions. However, there is minimal information about the prevalence of N. ...201122112976
seroprevalence of neospora caninum infection on dairy cattle in farms from southern romania.abstract neospora caninum, a coccidian parasite closely related to toxoplasma gondii, is one of the major causes of abortion in cattle worldwide. conventional serological techniques, such as indirect fluorescent antibody test and enzyme-linked immunosorbent assay (elisa), are routinely used in adult animals and aborted fetuses for the detection of anti-n. caninum antibodies. in romania, infection with n. caninum in cattle has been reported recently, but only in limited areas from the north and ...201121955278
neospora caninum in bovine fetuses of minas gerais, brazil: genetic characteristics of rdna.neosporosis is an important cause of abortion in cattle and information on their genetics and host parasite relationships are desirable. neospora caninum samples obtained from 24 bovine fetuses from minas gerais, were genetically analyzed in part of the rdna region, coding for rrnas. previously, brain, heart, liver, skeletal muscle, lung, kidney, spleen, thymus, lymph nodes, ovary or testis, uterus and skin of the ear were analyzed by conventional histopathology and immunohistochemistry. of thes ...201122166381
extensive production of neospora caninum tissue cysts in a carnivorous marsupial succumbing to experimental neosporosis.abstract: experimental infections of sminthopsis crassicaudata, the fat-tailed dunnart, a carnivorous marsupial widely distributed throughout the arid and semi-arid zones of australia, show that this species can act as an intermediate host for neospora caninum. in contrast to existing models that develop relatively few n. caninum cysts, dunnarts offer a new animal model where active neosporosis is dominated by tissue cyst production. the results provide evidence for a sylvatic life cycle of n. c ...201121635733
prevalence and risk factors of giardia duodenalis in dogs from romania.the protozoan giardia duodenalis is a mammalian-infecting parasite that produces diarrhoea and malabsorption in its hosts. a survey to investigate canine infections with g. duodenalis in romania was undertaken between june 2008 and december 2009. the objectives of the study were to (i) estimate the prevalence of infection in different dog populations (kennels, shelters, shepherd, household) using microscopy and a commercially available enzyme-linked immunosorbent assay (elisa) test kit; (ii) to ...201121899952
transplacental transmission in cattle: is toxoplasma gondii less potent than neospora caninum?we compared the transplacental-transmission ability of toxoplasma gondii and neospora caninum in cattle. one uninfected pregnant heifer served as control, while three were inoculated with n. caninum k9wa strain and four with t. gondii rh strain at their midgestational period. both infected groups showed clinical signs and antibodies either to n. caninum or t. gondii, while the control animal was normal. two (50%) toxoplasma dams aborted on days 6 and 11 postinoculation. t. gondii tachyzoites wer ...201121203773
occurrence of anti-neospora caninum and anti-toxoplasma gondii igg antibodies in goats and sheep in western maranhão, brazil.neosporosis and toxoplasmosis are parasitic diseases which can cause reproductive problems in goats and sheep. the current study aimed to determine the occurrence of anti-neospora caninum and anti-toxoplasma gondii igg antibodies in goats and sheep from the districts of amarante do maranhão and buritirana, imperatriz microregion, western area of maranhão state, northeastern brazil, and to assess factors associated to infection by these etiologic agents. blood samples from 110 animals (46 goats a ...201122166386
Molecular pathology, taxonomy and epidemiology of Besnoitia species (Protozoa: Sarcocystidae).Until recently, besnoitiosis has been a neglected disease of domestic animals. Now, a geographic expansion of the causing protozoan parasite Besnoitia besnoiti in livestock has been recognized and the disease in cattle is considered emerging in Europe. Bovine besnoitiosis leads to significant economic losses by a decline in milk production, sterility, transient or permanent infertility of bulls, skin lesions and increase of mortality in affected cattle population. Phylogenetically, the Besnoitia ...201121906696
prevalence of neospora caninum and toxoplasma gondii antibodies in sera from camels (camelus dromedarius) in riyadh province, saudi arabia.from april to december 2010, blood samples were collected from 412 healthy camels in riyadh province, saudi arabia and used to evaluate serological screening for neospora caninum and toxoplasma gondii infection by an indirect fluorescent antibody test (ifat). of the 412 camels, antibodies to n. caninum were found in sixteen in titers of 1:20 and in seven in titers of 1:40 using whole n. caninum tachyzoites as ifat slide (vmrd inc., pullman, wa 99163, usa). antibodies to t. gondii were found in n ...201121980764
molecular detection of coxiella burnetii and neospora caninum in equine aborted foetuses and neonates.abortion, stillbirth and neonatal death are major causes of equine mortality and cause severe economic loss to the equine industry. the present study was based on a complete necropsy protocol associated with classical microbiological examinations and molecular biology on 407 cases of abortion, stillbirths and neonate death. based on this retrospective survey, "less common" abortive infectious agents were characterised by molecular tools in nine independent cases of abortion or neonate mortality. ...201122130310
identification of novel proteins in neospora caninum using an organelle purification and monoclonal antibody approach.neospora caninum is an important veterinary pathogen that causes abortion in cattle and neuromuscular disease in dogs. neospora has also generated substantial interest because it is an extremely close relative of the human pathogen toxoplasma gondii, yet does not appear to infect humans. while for toxoplasma there are a wide array of molecular tools and reagents available for experimental investigation, relatively few reagents exist for neospora. to investigate the unique biological features of ...201121483743
Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples.The current study describes the development of a set of 5 multiplex polymerase chain reaction (mPCR) assays for the simultaneous detection of abortive infection agents in bovine fetal tissues, including Brucella spp., Leptospira spp., and Campylobacter fetus (mPCR1); Hammondia heydorni, Neospora caninum, and Toxoplasma gondii (mPCR2); Coxiella burnetii and Chlamydophila psittaci (mPCR3); Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum (mPCR4); and Bovine viral diarrhea virus ...201121908306
in vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of neospora caninum.abstract: in this study, we examined the in vitro invasion and proliferation capacities of the nc-liv and ten spanish neospora caninum isolates (nc-spain 1 h - nc-spain 10). the invasion rate was determined as the number of tachyzoites that completed their internalisation into marc-145 cells at 2, 4, and 6 h post-inoculation (pi). the proliferation rate was evaluated by determining the doubling time during the exponential proliferation period. significant differences in the invasion rates of the ...201121345202
The immune enhancement of a novel soy lecithin/ß-glucans based adjuvant on native Neospora caninum tachyzoite extract vaccine in mice.Efficient, cost-effective and safe Th1-immunity-inducing vaccine formulations are paramount for achieving protection against Neospora caninum. In this study, a new adjuvant (Providean-AVEC(®)) was used in the development of a N. caninum vaccine and evaluated in a mouse model. Soluble N. caninum tachyzoite native protein extract (sNcAg) was selected as vaccine antigen based on its capacity to activate production of pro-inflammatory cytokines on dendritic cells. Vaccines containing 4 and 0.4µg of ...201122178728
serological survey of neospora caninum in small ruminants from pernambuco state, brazil.neospora caninum infection is an important cause of bovine abortion as well as neonatal mortality in goats and sheep. a serological survey for antibodies against n. caninum in goats and sheep was carried out in the municipality of ibimirim, pe, northeastern brazil. the imunnofluoresce antibody test showed that 26.6% (85/319) of the goats and 64.2% (52/81) of the sheep were positive. serologic reactivity was associated with age in goats (p < 0.01) and sheep (p > 0.05), with increasing rates in ol ...201121961757
seroprevalence of toxoplasma gondii and neospora caninum in dairy goats from romania.little information is available about the seroprevalence of toxoplasma gondii and neospora caninum infections in goats in romania and even in europe. during 2007-2010, 735 serum samples were collected from dairy goats located in 4 historical regions (crişana, maramureş, transylvania and muntenia) of romania. sera were analyzed for t. gondii and n. caninum antibodies (igg type) by enzyme-linked immunosorbent assay (elisa) using two commercial kits (chekit toxotest antibody elisa and chekit neospo ...201122177331
prevalence of neospora caninum antibodies in sheep and goats in pakistan.abstract the purpose of the present study was to obtain seroepidemiological information on the neospora caninum infection status of sheep and goats in different areas of punjab province and azad kashmir (pakistan). a cross-sectional study, using a competitive elisa showed an overall 27.7% (35 of 128) (95% ci ? 7.7%) and 8.6% (13 of 142)(95% ci ? 4.6%) seroprevalence of n. caninum antibodies in sheep and goats, respectively. the difference in seroprevalence between sheep and goat populations wa ...201121854220
an applied printing immunoassay with recombinant nc-sag1 for detection of antibodies to neospora caninum in cattle.neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. neospora caninum surface antigen 1 (nc-sag1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. the current study describes the development and evaluation of an antigen print immunoassay (apia) with recombinant nc-sag1 for the detection of specific antibodies to n. caninum in cattle. the concordance between apia and a commercial enzyme-linked immunosorbent as ...201121908357
first report of neospora caninum infection in cattle in sudan.a cross-sectional survey was conducted in sudan to determine sero-prevalence and risk factors associated with neospora caninum infection in non-vaccinated dairy herds and to assess importance of the disease. blood samples were collected from a total of 262 animals from 25 herds. sera were tested for antibodies against n. caninum using elisa test. the prevalence rates of n. caninum antibodies in cattle were high both at herd level (44%) and at individual animal level (10.7%). herd level infection ...201121898183
genetic mapping identifies novel highly protective antigens for an apicomplexan parasite.apicomplexan parasites are responsible for a myriad of diseases in humans and livestock; yet despite intensive effort, development of effective sub-unit vaccines remains a long-term goal. antigenic complexity and our inability to identify protective antigens from the pool that induce response are serious challenges in the development of new vaccines. using a combination of parasite genetics and selective barriers with population-based genetic fingerprinting, we have identified that immunity agai ...201121347348
emergence of bovine ehrlichiosis in belgian cattle herds.bovine ehrlichiosis is a tick-borne rickettsial disease caused by anaplasma phagocytophilum. the disease can also be transmitted to humans. outbreaks in cattle have been described in many european countries. in belgium, infections caused by a. phagocytophilum have been reported in humans and dogs; however, this paper details the first report of ehrlichiosis in cattle herds in belgium. the first case described was in a dairy herd located in eastern belgium. clinical signs included hyperthermia, p ...201121771546
prevalence and co-infection of toxoplasma gondii and neospora caninum in apodemus sylvaticus in an area relatively free of cats.summarythe protozoan parasite toxoplasma gondii is prevalent worldwide and can infect a remarkably wide range of hosts despite felids being the only definitive host. as cats play a major role in transmission to secondary mammalian hosts, the interaction between cats and these hosts should be a major factor determining final prevalence in the secondary host. this study investigates the prevalence of t. gondii in a natural population of apodemus sylvaticus collected from an area with low cat densi ...201121756421
results of parasitological examinations of faecal samples from cats and dogs in germany between 2003 and 2010.in a retrospective study, the results of parasitological examinations of faecal samples from 8,560 cats and 24,677 dogs between january 2003 and december 2010 in germany were analysed. 30.4 % of the examined dogs and 22.8 % of the cats were infected with endoparasites. the examination of the faecal samples from dogs revealed stages of giardia spp. (18.6 %), toxocara canis (6.1 %), toxascaris leonina (0.6 %), ancylostomatidae (2.2 %), trichuris vulpis (1.2 %), capillaria spp. (1.3 %), crenosoma v ...201121739375
presence of antibodies against toxoplasma gondii, neospora caninum and leishmania infantum in dogs from piau+¡.this study aimed to evaluate the presence of antibodies against neospora caninum, toxoplasma gondii and leishmania infantum in dogs attended at the veterinary hospital of the federal university of piau+¡, northeastern brazil, where there are no reports of the occurrence of n. caninum and t. gondii in dogs. serum samples from 530 dogs of genders, different ages and breeds from the municipality of teresina and nearby towns were analyzed using three indirect fluorescent antibody tests, each one tar ...201121722484
seroprevalence of toxoplasma gondii and neospora caninum in slaughtered pigs in the czech republic.summaryin the czech republic, sera from 551 clinically healthy adult slaughtered pigs (females, 6-8 months old) were collected during the first half of june in 2010. sera were tested for toxoplasma gondii-specific igg antibodies by an enzyme-linked immunosorbent assay; samples with more than 50% s/p were considered as positive. the same samples were also analysed for neospora caninum antibodies using a commercial competitive-inhibition enzyme-linked immunosorbent assay; samples with more than 30 ...201121819636
seroprevalence of neospora caninum in free-range chickens (gallus domesticus) from the americas.neospora caninum and toxoplasma gondii are biologically and morphologically similar coccidians with canids as definitive hosts for n. caninum and felids for t. gondii. feral chickens have been used as indicators of soil contamination with t. gondii oocysts because they feed from ground. in the present study we studied seroprevalence of n. caninum in free range chickens from different countries in america as an indicator of soil contamination due to n. caninum oocysts. antibodies to n. caninum we ...201121676546
cytokine gene expression at the materno-foetal interface after experimental neospora caninum infection of heifers at 110ôçâdays of gestation.neospora caninum is a major cause of abortion in cattle, but the reasons why only some animals abort remain unclear. the immunological control of the parasite in the placenta or by the foetus could be the key to determining the mechanism of abortion and/or transplacental transmission to the foetus. in this study, cytokine gene expression, analysed by real-time rt-pcr, at the maternal (caruncle) and foetal placenta (cotyledon) of heifers infected at 110ôçâdays of gestation by intravenous inoculat ...201121711362
presence of toxoplasma gondii and neospora caninum dna in the brain of wild birds.toxoplasma gondii infections are prevalent in many avian species and can cause mortality in some bird hosts. although t. gondii has been isolated from various species of birds, the role of many different species of wild birds in the epidemiology of t. gondii remains unknown. neospora caninum, a closely related parasite to t. gondii, has been recently confirmed to infect domestic chickens and wild birds such as house sparrows (passer domesticus). the present study reports the presence of t. gondi ...201121831525
epizootiologic investigations of selected abortive agents in free-ranging alpine ibex (capra ibex ibex) in switzerland.in the early 2000s, several colonies of alpine ibex (capra ibex ibex) in switzerland ceased growing or began to decrease. reproductive problems due to infections with abortive agents might have negatively affected recruitment. we assessed the presence of selected agents of abortion in alpine ibex by serologic, molecular, and culture techniques and evaluated whether infection with these agents might have affected population densities. blood and fecal samples were collected from 651 ibex in 14 col ...201121719818
neosporosis in animals--the last five years.neospora caninum is a protozoan parasite of animals. until 1988, it was misdiagnosed as toxoplasma gondii. since its first recognition in 1984 in dogs and the description of a new genus and species neospora caninum in 1988, neosporosis has emerged as a serious disease of cattle and dogs worldwide. abortions and neonatal mortality are a major problem in livestock operations and neosporosis is a major cause of abortion in cattle. this review is focused on current status of neosporosis in animals b ...201121704458
epidemiology and impact of neospora caninum infection in three queensland tropical dairy herds.this study investigated the epidemiology of neospora caninum in three tropical dairy herds in north queensland, australia. all animals in the herds were bled, and the sera were tested by elisa for n. caninum antibodies. herd records were examined, and the number of calves carried to term and the number of abortions which occurred over the lifetime of each animal were recorded to determine the abortion rate for each animal. pedigrees were constructed for two of the herds to investigate whether ve ...201121736635
transgenic neospora caninum strains constitutively expressing the bradyzoite ncsag4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice.at present, there is no effective treatment or vaccine to prevent vertical transmission or abortion associated with neospora caninum infection in cattle. different vaccine formulations have been assayed, and live vaccines have shown the most promising results in terms of protection. previously, transgenic n. caninum tachyzoites expressing the bradyzoite stage-specific ncsag4 antigen in a constitutive manner (nc-1 sag4(c)) were obtained and showed a reduced persistence of parasite in inoculated m ...201121816191
survey for natural neospora caninum infection in wild and captive birds.neospora caninum is a protozoan parasite that presents worldwide distribution and is mainly implicated as responsible for bovine abortion. although the presence of birds in cattle-raising properties is positively correlated to higher infection rates, very little has been described about the role of these animals in the parasite's life cycle. in that sense, this work aimed to investigate the serological and histological positivity of different avian species sampled in its natural habitat or in ca ...201121680099
comparative efficacy of immunization with inactivated whole tachyzoites versus a tachyzoite-bradyzoite mixture against neosporosis in mice.summarythe worldwide economic impact of neospora caninum infection has caused the development of effective vaccines to become one of the main goals in the field of neosporosis research. in this study, the protection conferred by antigens from inactivated whole tachyzoites (tz) and a tachyzoite-bradyzoite mixture (tz-bz) of n. caninum (nc-spain7 isolate) incorporated into a water-in-oil emulsion (w/o) and aluminium hydroxide-ginseng extract (al/g) was evaluated in mouse models of congenital and c ...201121810300
seroprevalence of neospora caninum in dairy cattle from north-west and centre of romania.neosporosis is a disease that mainly affects cattle in both dairy and beef herds. the main definitive host of this parasite is the dog. since 1984 and its first description a large number of data were published worldwide on this parasite. in romania, the research regarding this parasite is limited. the purpose of this study was to determine the seroprevalence of neospora caninum infection in dairy cattle from six regions in north-western romania and to evaluate the intensity of infection in diff ...201122091468
Parasite limiting macrophages promote IL-17 secretion in naive bovine CD4? T-cells during Neospora caninum infection.Neospora caninum infects bovine hosts giving rise to pro-inflammatory immune responses that can result in foetal death or spontaneous abortion, this appears to be mediated by the actions of IFN-? on cell activation and migration/trafficking. Yet successful vaccination or natural immunity is also strongly correlated with IFN-? production. We utilised in vitro infection of bovine macrophages to prime naive T-cell responses. Naive T-cells in contact with infected macrophages produce both IFN-? and ...201122005586
Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand.The objectives of this study were to investigate the risk factors of Neospora caninum infection in dogs and cats found in dairy herds in western Thailand. A case-control study was conducted in dairy herds in three western provinces including Nakhon Pathom, Ratchaburi and Kanchanaburi. Blood samples of pets from 14 positive dairy herds and 26 herds from negative neighbouring farms which were randomly selected, in total blood samples from dogs and cats from 40 herds were collected and examined for ...201122193939
artinm, a d-mannose-binding lectin from artocarpus integrifolia, plays a potent adjuvant and immunostimulatory role in immunization against neospora caninum.artinm and jacalin (jac) are lectins from the jackfruit (artocarpus integrifolia) that have important role in modulation of immune responses to pathogens. neospora caninum is an apicomplexa parasite that causes neuromuscular disease in dogs and reproductive disorders in cattle, with economic impact on the livestock industry. hence, we evaluated the adjuvant effect of artinm and jac in immunization of mice against neosporosis. six c57bl/6 mouse groups were subcutaneously immunized three times at ...201122001880
serological survey of neospora caninum infection in cattle herds from western romania.abstract serum samples from 376 randomly selected adult cattle, from 25 farms located in 3 counties (arad, bihor, and timiş) from western romania, were sampled for neospora caninum antibodies using a commercial elisa-kit (bio k 218, bio-x diagnostics, jemelle, belgium). seroprevalence values and risk factors for neosporosis (cow age, breed, herd size, farming system, previous abortion, and number of farm dogs) were examined using a generalized linear mixed model with a binomial distribution. o ...201122150344
identification of two novel coccidian species shed by california sea lions (zalophus californianus).abstract routine fecal examination revealed novel coccidian oocysts in asymptomatic californa sea lions (zalophus californianus) in a rehabilitation facility. coccidian oocysts were observed in fecal samples collected from 15 of 410 california sea lions admitted to the marine mammal center between april 2007 and october 2009. phylogenetic analysis using the full its-1 region, partial small subunit 18s rdna sequence and the apicomplexa rpob region identified 2 distinct sequence clades, referred ...201122091999
factors associated with infection by neospora caninum in dogs in brazil.from august 2006 to 2008, 411 dogs in northeastern brazil were evaluated for seropositivity to neospora caninum. the dogs were clinically examined, and their owners were interviewed about the conditions in which the animals were maintained in order to assess the factors associated with infection by this parasite. a serum sample was taken from each dog for serological examination in an indirect fluorescent antibody test for n. caninum. the yates' chi-square test or fisher's exact test was used to ...201122015062
neospora caninum: comparative gene expression profiling of neospora caninum wild type and a temperature sensitive clone.to understand the genetic basis of virulence, gene expression profiles of a temperature-sensitive clone (ncts-8, relatively avirulent) and its wild type (nc-1) of neospora caninum were characterized and compared using a high-density microarray with approximately 63,000 distinct oligonucleotides. this microarray consists of 5692 unique n. caninum sequences, including 1980 tentative consensus sequences and 3712 singleton ests from the tigr n. caninum gene index (ncgi, release 5.0). each sequence w ...201121963790
neospora caninum: in vivo and in vitro treatment with artemisone.neosporosis caused by neospora caninum has global economic, clinical, and epidemiological impacts, mainly in the cattle industry. currently, there is no useful drug for treatment of neosporosis. this publication is the first to describe the significant benefits that artemisone has on neospora infections both in vitro and in vivo. artemisone is a new semi-synthetic 10-alkylamino artemisinin that is superior to other artemisinin derivatives in terms of its significantly higher antimalarial activit ...201122260899
experimental inoculation of neospora caninum in pregnant water buffalo.the aim of this study was to characterize the pathogenesis of neospora caninum in experimentally inoculated pregnant water buffalo (bubalus bubalis). twelve mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to n. caninum by indirect fluorescent antibody test (ifat) were involved. ten females were intravenously inoculated with 10(8) tachyzoites of nc-1 strain at 70 (n=3) or 90 (n=7) days of pregnancy (dp). two control animals were inoculated with placebo ...201122244534
a study of some infectious causes of reproductive disorders in cattle owned by resource-poor farmers in gauteng province, south africa.two hundred and thirty-nine cattle from gauteng province in south africa were tested for various pathogens causing reproductive diseases includingbovine viral diarrhoea/mucosal disease (bvd/md) virus, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (ibr/ipv) virus, neospora caninum and brucella abortus usingvarious tests. for bvd/md virus, 49.37% tested positive, 74.47% for ibr/ipv virus, 8.96% for neospora caninum and 3.8% for brucella abortus. the result for brucella abort ...201122616434
neospora caninum and toxoplasma gondii in brain tissue of feral rodents and insectivores caught on farms in the netherlands.we investigated the presence of both neospora caninum and toxoplasma gondii in 250 brain tissue samples from 9 species of feral rodents and insectivores caught on 10 organic farms in the netherlands in 2004. collected samples were conserved in 4% paraformaldehyde solution and analysed by real-time pcr. for n. caninum, 31 samples originating from 6 species tested positive (12.4%): common shrews (33.3%), wood mice (17.6%), harvest mice (16.7%), house mice (15.4%), white-toothed shrews (10.8%) and ...201121958437
Refresh
firstprev
...111213141516171819
nextlast
Displaying items 1201 - 1300 of 1864