Publications

production of biologically active recombinant bovine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae.	the full-length bovine interferon-gamma (bifn-gamma) cdna, including the secretion signal peptide coding region was recloned into baculovirus transfer vectors pacym1 and pbm050. these vectors were co-transfected with autographa californica nuclear polyhedrosis virus (acnpv) or bombyx mori nuclear polyhedrosis virus (bmnpv) dna into spodoptera frugiperda cells (sf21ae) and bombyx mori cells (bmn), respectively. the recombinant viruses, named acbifn-gamma and bmbifn-gamma, were then recovered. rec ...	2001	11145838
comparative effects of a genetically engineered insect virus and a growth-regulating insecticide on microbial communities in aquatic microcosms.	the effects of a genetically engineered insect baculovirus on indigenous aquatic microbial communities were determined in closed, recirculating aquatic microcosms, and compared with the effects of a natural strain of the virus and of a growth-regulating insecticide, dimilin. the recombinant virus was a nuclear polyhedrosis virus (npv) of the spruce budworm (choristoneura fumiferana (cf)) with a lacz marker gene inserted into the egt region of the cfnpv. the natural virus was ireland strain cfnpv ...	2001	11161682
high-level expression of human acidic fibroblast growth factor and basic fibroblast growth factor in silkworm (bombyx mori l.) using recombinant baculovirus.	a hybrid of autographa californica nuclear polyhedrosis virus and bombyx mori nuclear polyhedrosis virus, which is infectious to both spodoptera frugiperda and bombyx mori, was prepared in our previous study. two recombinant hybrid baculoviruses, carrying cdnas of human acidic and basic fibroblast growth factors, respectively, were successfully constructed in this study, for the large-scale production of human afgf and bfgf using silkworm as host. these recombinant viruses were used to inoculate ...	2001	11162406
diagnostic immunoassays for tick-borne encephalitis virus based on recombinant baculovirus protein expression.	the baculovirus expression system that utilizes autographa californica nuclear polyhedrosis virus was used to express the highly antigenic envelope protein e of a tick-borne encephalitis (tbe) complex virus, as well as a c-terminally truncated form of protein e (etr). the recombinant proteins were produced with a histidine-tag at their carboxy-terminus. protein purification by nickel agarose chromatography resulted in high concentrations of pure etr protein, but only poor yields of e protein. th ...	2001	11164488
[analysis and expression of hyphantria cunea nuclear polyhedrosis virus sod gene].	the sequencing results indicated that hyphantria cunea nuclear polyhedrosis virus (hcnpv) sod gene open reading frame of 456 nt encoding protein of 151 amino acid, was identified to that of bombyx mori nuclear polyhedrosis virus (bmnpv), and exhibited 97.2% homology at nucletde level to that of autographa californica nuclear polyhedrosis virus (acnpv), three amino acid residues difference in amino acid level with acnpv sod. the essential amino acid residues for the construction and active could ...	2001	12549022
[package of bombyx mori nuclear polyhedrosis virus with heterogoneous polyhedring protein].	cloning the segment of polyhedrin gene which was separated from acmnpv genome dna by pcr method into transfer vector pbacpak8, we got recombinant transfer vector poac, then contransfecting bmn cells with the vector and linear virus bm-bacpak6, harvested recombinant virus hp-bmnpv which can produce polyhedrin but no blue plaques. analysing of recombinant virus from polyhedrin, recombinant viral dna and polyhedra, we confirmed that polyhedring of acmnpv can be not only high-level expressed in bmn ...	2002	12557348
baculovirus studies in new, indigenous lepidopteran cell lines.	eight lepidopteran cell lines were established recently and their susceptibility to different insect viruses was studied. two spodoptera litura cell lines from the larval and pupal ovaries, were found highly susceptible to s. litura nuclear polyhedrosis virus (slnpv, 5-6 x 10(6) npv/ml). the helicoverpa armigera cell line from the embryonic tissue was highly susceptible to h. armigera npv (hanpv, 6.3 x 10(6) npv/ml). these in vitro grown slnpv and hanpv caused 100% mortality to respective 2nd in ...	2002	12561971
foreign insect hormones stimulating the transcription of the ie-1 promoter of bombyx mori nuclear polyhedrosis virus in vivo and in vitro.	via a transient expression assay system, an experimental study was undertaken to characterize the effects of insect ecdysone and juvenile hormone analogue on the transient expression of the luciferase gene under the control of the immediate-early gene (ie-1) promoter of bombyx mori nuclear polyhedrosis virus. the results demonstrated that the transcriptional activity of the ie-1 promoter was increased to a certain extent by different insect hormone treatments in uninfected insect cells or fifth ...	2002	12224632
altering the surface properties of baculovirus autographa californica npv by insertional mutagenesis of the envelope protein gp64.	the envelope protein gp64 of the baculovirus autographa californica nuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein both mediates ph-dependent membrane fusion and binds to host cell receptors. surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and powerful tool in molecular biology. to improve further the presentation of peptides on the surface of ...	2002	12230557
mass production of polyhedral occlusion bodies of npv of helicoverpa armigera in relation to dose, age and larval weight.	a significant difference was noticed in the yield of polyhedral occlusion bodies (pobs) in various larval instars of h. armigera when three different doses of the nuclear polyhedrosis virus (npv) were administered. the yield of pobs from a single larva ranged from 0.35 x 10(6) to 25033.33 x 10(6) with a mean of 18422.33 x 10(6) for fourth instar inoculated. positive correlation existed between larval weight and number of pobs recovered. the regression analysis indicated pobs recovered responded ...	2002	12597558
significant increase in recombinant protein production of a virus-infected sf-9 insect cell culture of low moi under low dissolved oxygen conditions.	spodoptera frugiperda sf-9 insect cells were infected with recombinant autographa californica nuclear polyhedrosis virus at a low multiplicity of infection (moi) (0.1), and the effect of dissolved oxygen (do) on the production of a polyhedrin promoter-driven recombinant protein (beta-galactosidase), intrinsic proteases (carboxyl and cysteine proteases), and the virus was determined. the do concentrations used in the present study were 45%, 25%, 5%, and 1.3% of air saturation. at 5% do the cell g ...	2002	16233329
[identification of functional region of helicase gene promoter in bombyx mori nuclear polyhedrosis virus].	dna helicases are essential for replication of baculoviruses. it was found that the helicase gene promoter of bombyx mori nuclear polyhedrosis virus, including 510 bp upstream of atg, had both early and late rna initiation sites and could be recognized by cellular rna polymerase. transient expression assays in uninfected sf-21 cells indicated that the helicase gene promoter could be classified as a delayed-early gene promoter. deletion analysis by pcr showed that the regulation region of its bas ...	2002	12198556
production of recombinant ovine interferon tau using a bombyx mori nuclear polyhedrosis baculovirus expression system.	ovine interferon tau (oifn-tau) is an embryonic protein of critical importance in the establishment of pregnancy in the sheep. we have produced recombinant (r) oifn-tau using a baculovirus expression system and demonstrated the biological activity of the protein produced. bombyx mori larvae were infected with b. mori nuclear polyhedrosis virus (bmnpv), modified by inserting a cdna coding for oifn-tau downstream of the strong polyhedron promoter. following infection, antiviral activity of the hae ...	2002	12212938
baculovirus infection raises the level of tata-binding protein that colocalizes with viral dna replication sites.	during the infection cycle of autographa californica multicapsid nuclear polyhedrosis virus, the tata-binding protein (tbp) of the insect host cell likely participates in early viral transcription, which is mediated by the host rna polymerase ii. however, the role of tbp in late and very late viral transcription, which is accomplished by an alpha-amanitin-resistant rna polymerase, is unclear. we observed a dramatic increase of tbp protein during the late phases of infection. tbp mrna levels, how ...	2002	12368354
insect cells as a factory to produce adeno-associated virus type 2 vectors.	recombinant adeno-associated viruses (raav) are produced transiently in mammalian cells usually by cotransfecting two or three plasmids containing aav genes, adenovirus helper genes, and a vector genome. expansion and transfection of adherent cells limit the scale of raav production. efficient transfection is performed with cells on solid support media such as tissue culture plates. a large animal study or a human clinical trial may require 10(15) particles of vector, depending on dose. to gener ...	2002	12427305
functional properties of a rainbow trout cyp3a27 expressed by recombinant baculovirus in insect cells.	cytochrome p450 3a27 (cyp3a27) is highly expressed in liver and intestine of rainbow trout (oncorhynchus mykiss). in many animal species, the intestine and liver are responsible for the first-pass metabolism of a wide range of xenobiotics. to help determine its physiological role, the catalytic capabilities of cyp3a27 protein were examined. an open reading frame of cyp3a27 in pfastbac donor plasmid was transferred to the baculovirus genome (bacmid dna) through tn7 site-specific transposition in ...	2002	12433811
expression of biologically active recombinant equine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae.	the full-length equine interferon-gamma (eifn-gamma) cdna, including the secretion signal peptide coding region, was recloned into baculovirus transfer vector pacym1. this vector was co-transfected with autographa californica nuclear polyhedrosis virus dna or hybrid nuclear polyhedrosis virus dna into spodoptera frugiperda cells. the recombinant viruses, named aceifn-gamma and hyeifn-gamma, were then recovered. recombinant eifn-gamma (reifn-gamma) was accumulated in the culture fluid of the acei ...	2002	12445800
use of an n-terminal half truncated ie1 as an antagonist of ie1, an essential regulatory protein in baculovirus.	an immediate-early gene product of baculovirus, ie1, is essential for viral gene expression and for viral dna replication. it has been demonstrated for autographa californica nuclear polyhedrosis virus (acnpv) that the c-terminal region of ie1 is required for dimerization. and the acidic n-terminal region of ie1 has been identified as the activation domain. we constructed an n-terminal 267 amino acid (a.a.) truncated mutant of bombyx mori nuclear polyhedrosis virus (bmnpv) ie1, which was defecti ...	2002	12457979
interaction of heliothis armigera nuclear polyhedrosis viral capsid protein with its host actin.	in order to find the cellular interaction factors of the heliothis armigera nuclear polyhedrosis virus capsid protein vp39, a heliothis armigera cell cdna library was constructed. then vp39 was used as bait. the host actin gene was isolated from the cdna library with the yeast two-hybrid system. this demonstrated that vp39 could interact with its host actin in yeast. in order to corroborate this interaction in vivo, the vp39 gene was fused with the green fluorescent protein gene in plasmid pegfp ...	2002	12470589
inhibitor of apoptosis protein from orgyia pseudotsugata nuclear polyhedrosis virus provides a costimulatory signal required for optimal proliferation of developing thymocytes.	the inhibitors of apoptosis proteins (iaps) constitute a family of endogenous inhibitors that control apoptosis in the cell by inhibiting caspase processing and activity. iaps are also implicated in cell division, cell cycle regulation, and cancer. to address the role of iaps in thymus development and homeostasis, we generated transgenic mice expressing iap generated from the baculovirus orgyia pseudotsugata nuclear polyhedrosis virus (opiap). developing thymocytes expressing opiap show increase ...	2002	11823509
development of a competitive enzyme-linked immunosorbent assay for detection of turkey coronavirus antibodies.	a competitive enzyme-linked immunosorbent assay (celisa) was developed for detection of turkey coronavirus (tcv) antibodies. the celisa utilized a recombinant baculovirus (autographa californica nuclear polyhedrosis virus)-expressed tcv nucleocapsid (n) protein and biotin-labeled tcv n protein-specific monoclonal antibody. sensitivity and specificity of the celisa for detection of tcv antibodies were determined by comparison with the indirect fluorescent antibody test (ifat) with 1269 reference, ...	2002	12061642
timing of transmission and the evolution of virulence of an insect virus.	we used the nuclear polyhedrosis virus of the gypsy moth, lymantria dispar, to investigate whether the timing of transmission influences the evolution of virulence. in theory, early transmission should favour rapid replication and increase virulence, while late transmission should favour slower replication and reduce virulence. we tested this prediction by subjecting one set of 10 virus lineages to early transmission (early viruses) and another set to late transmission (late viruses). each linea ...	2002	12061960
baculovirus as mammalian cell expression vector for gene therapy: an emerging strategy.	the monopoly of insect cells to host baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) as a eukaryotic gene expression system has been shattered with the growing evidence that it also infects mammalian cells in culture. although acmnpv fails to replicate in vertebrate cells, it does express alien genes with levels of expression that are dependent on the strength of the promoter used to drive transcription of the foreign gene. it also has been reported that the recom ...	2002	12095297
intron-dependent stimulation of marker gene expression in cultured insect cells.	we tested in a systematic fashion the effect of an intron on the level of luciferase expression in cultured c6/36 aedes albopictus cells. the intron was inserted in both orientations, upstream and downstream of the luciferase coding region in two different luciferase expression vectors. the two parental luciferase expression vectors differed only in their promoters, one containing the drosophila melanogaster actin5c promoter and the other the autographa californica nuclear polyhedrosis virus hr5 ...	2002	11841506
characterization of a truncated soluble form of the baculovirus (acmnpv) major envelope protein gp64.	a truncated tagged form of the autographica californica multiple nuclear polyhedrosis virus major surface glycoprotein, gp64, has been expressed using the baculovirus expression system and purified to homogeneity by immune-affinity chromatography. the protein, which is responsible for virus-cell fusion, was a trimer in solution and retained this oligomeric form at ph 5, the ph of fusion. circular dichroism spectroscopy indicated a protein with mixed alpha-helix and beta-sheet content that did no ...	2002	11858713
nuclear ie2 structures are related to viral dna replication sites during baculovirus infection.	the ie2 gene of autographa californica multicapsid nuclear polyhedrosis virus is 1 of the 10 baculovirus genes that have been identified as factors involved in viral dna replication. ie2 is detectable in the nucleus as one of the major early-expressed proteins and exhibits a dynamic localization pattern during the infection cycle (d. murges, i. quadt, j. schröer, and d. knebel-mörsdorf, exp. cell res. 264:219-232, 2001). here, we investigated whether ie2 localized to regions of viral dna replica ...	2002	11967334
in vivo expression and immunological studies of the 42-kilodalton carboxyl-terminal processing fragment of plasmodium falciparum merozoite surface protein 1 in the baculovirus-silkworm system.	the 42-kda carboxyl-terminal processing fragment of plasmodium falciparum merozoite surface protein 1 (msp-1(42)) is an anti-erythrocytic stage malaria vaccine candidate. in this study, msp-1(42) was expressed by using the bombyx mori nuclear polyhedrosis virus-silkworm expression system, and the antigenicity and immmunogenicity of the recombinant protein, bmp42, were evaluated. the average yield of bmp42, as determined by a sandwich enzyme-linked immunosorbent assay (elisa), was 379 microg/ml o ...	2002	12010962
[cloning and sequencing of p35 gene from bombyx mori nuclear polyhedrosis virus strain shandong].	according to the 5' and 3' untranslated region sequences of p35 gene from bombyx mori nuclear polyhedrosis virus isolate t3, a pair of primers was designed employing computer analysis. with the primers,polymerase chain reaction (pcr) was performed and a 1080 bp fragment was obtained from bmnpv strain shandong. sequencing result showed that it was p35 gene (genbank accession number: ay157746), and it included an open reading frame of 900 bp, encoding 299 amino acids with mr=34.91 kda and pi=6.40. ...	2003	15639959
expression of dopamine d1 receptor in sf9 insect cells and agonism of l-12-chloroscoulerine on recombinant d1 receptor.	to express dopamine d1 receptor in baculovirus-sf9 cell system, and to investigate the effects of l-12-chloroscoulerine (l-csl) on the recombinant d1 receptor (d1r).	2003	12617770
cloning, tissue distribution, and functional studies of a new cytochrome p450 3a subfamily member, cyp3a45, from rainbow trout (oncorhynchus mykiss) intestinal ceca.	in trout and mammals, the major extrahepatic expression site for cyp3a forms is in the intestine. a cdna encoding a new cyp3a subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (rt-pcr), followed by rapid amplification of cdna ends (race)-pcr. in a set of two primers for pcr, a consensus sequence in the highly conserved regions in 17 cyp3a sequences was used for one primer, and the second primer was designed based on adapter sequen ...	2003	12646270
large-scale production of porcine mature interleukin-18 (il-18) in silkworms using a hybrid baculovirus expression system.	in this report, a hybrid baculovirus expression system, which means a hybrid virus of the autographa californica nuclear polyhedrosis virus and the bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of porcine mature interleukin-18 (il-18) in silkworms. two recombinant hybrid baculoviruses containing cdna of the porcine precursor il-18 and the porcine caspase-1 were constructed and were used to infect silkworm larvae. after the co-infection of the two viruses, porcin ...	2003	12655117
molecular cloning and sequence analysis of the anticarsia gemmatalis multicapsid nuclear polyhedrosis virus gp64 glycoprotein.	the gp64 locus of anticarsia gemmatalis multicapsid nucleopolyhedrovirus isolate santa fe (agmnpv-sf) was characterised molecularly in our laboratory. to this end, we have located and cloned a agmnpv-sf genomic dna fragment containing the gp64 gene and sequenced the complete gp64 locus. nucleotide sequence analysis indicated that the agmnpv gp64 gene consists of a 1500 nucleotide open reading frame (orf), encoding a protein of 499 amino acids. of the seven gp64 homologues identified to date, the ...	2003	12680694
establishment and characterization of a continuous cell line from pupal ovaries of japanese oak silkworm antheraea yamamai guerin-meneville.	pupal ovaries of the wild oak silkworm antheraea yamamai guerin-meneville were cultured in mgm-448 (modified grace medium-448) medium containing 10% fetal bovine serum. after the primary culture was set up in 1988, a continuous cell line was obtained in 1991, designated as nises-anya-0611 (anya-0611). the population doubling time was 54 hrs. and 19 min. at 96 passages and 88 hrs. and 29 min. at 387 passages. spindle-shaped and spherical cells coexisted in the cell group. the cell line karyotype ...	2003	12892519
expression of vp2 gene protein of infectious bursal disease virus detected in korea.	the vp2 gene dna (1.4 kb in approximate) of a very virulent infectious bursal disease virus (vvibdv) chinju strain detected in chinju, korea was cloned into the bacmid, a baculovirus shuttle vector, through transposition of the gene from initially cloned pfastbachta plasmid, a baculovirus expression vector, and was subsequently expressed in spodoptera frugiperda (sf) cells. biological properties of the expressed vp2 subunit protein were characterized to aid in the development of genetically engi ...	2003	12913360
[connection of virus carriage and acute infection, caused by nuclear polyhedrosis virus in native populations of silkworm (lymantria dispar l., lym. lepid.)].	the detection rate of dna of nuclear polyedrosis virus in the caterpillar organism ranged from 28.6 to 73.2%, the detection rate of virion antigens ranged from 10.3 to 38.2%, and that of the virus polyhedron antigens ranged from 16.1 to 52.3% in 4 natural populations of gipsy moths in the territory of the novosibirsk region at the outbreak culmination time of a mass reproduction of the insects. an artificial activation of virus reproduction, in experiment, caused death of the insects at a level ...	2003	12924100
rna interference as a metabolic engineering tool: potential for in vivo control of protein expression in an insect larval model.	many ex vivo factors influence the yield of recombinant protein produced via acmnpv (autographa californica multiple nucleocapsid nuclear polyhedrosis virus) in trichoplusia ni (t. ni) larvae. among these are: the method of infection, the time of infection, the virus load, and the time of harvest. in vivo strategies, however, that attempt to manipulate host function in this and other expression systems have largely been ignored. in this work, rna interference (rnai) is shown as an effective meta ...	2003	12948752
[influence of host age on the epizootic of nuclear polyhedrosis virus of spodoptera litura].	through investigation of tracking infected spodoptera litura larvae, a study was carried out to observe the epizootic dynamics of nuclear virus disease of s. litura after virus release in different instar with concentration of 2.15 x 10(7) pibs.ml-1 in vegetable field. the results showed that with the age of s. litura increasing, the susceptibility to virus, mortality and death velocity decreased, the disease death sustained period of larvae obviously elongated, and the time that larval mortalit ...	2003	12974004
biological activity of recombinant bovine interferon tau using an autographa californica nuclear polyhedrosis virus expression system.	bovine interferon (bifn) tau, which plays a key role in maternal-fetal recognition of pregnancy, was expressed by an autographa californica nuclear polyhedrosis virus expression system. cdna coding bifntau was derived from cultured trophoblast cells. the recombinant (r) bifntau had high antiviral activity (1 x 10 (8) iu/mg) and the molecular weight of rbifntau was estimated to be 23 kda by western blotting analysis. we investigated the biological effect of rbifntau on prostaglandin (pg) f(2alpha ...	2003	14967893
increased mortality of gypsy moth lymantria dispar (l.) (lepidoptera: lymantriidae) exposed to gypsy moth nuclear polyhedrosis virus in combination with the phenolic gycoside salicin.	second instar gypsy moth, lymantria dispar (l.) (lepidoptera: lymantriidae), larvae suffered significantly greater mortality from aerially applied gypsy moth nuclear polyhedrosis virus (gypchek) when the virus was consumed on quaking aspen, populus tremuloides michx., versus red oak, quercus spp. l., foliage. laboratory assays in which various doses of gypchek and salicin (a phenolic glycoside present in aspen foliage) were tested in combination demonstrated that salicin significantly increased ...	2003	14977101
baculoviral polyhedrin as a novel fusion partner for formation of inclusion body in escherichia coli.	baculoviral polyhedrin, which originated from autographa californica nuclear polyhedrosis virus (acnpv), was employed for the first time as a novel fusion partner for expression of foreign proteins in an escherichia coli system. we characterized the expression of recombinant polyhedrin protein fused to green fluorescent protein (gfp). the polyhedrin fusion protein ( approximately 58 kda) was successfully expressed as an insoluble inclusion body comprising approximately 30% of the total cellular ...	2003	14574705
baculovirus capsid display: a novel tool for transduction imaging.	baculoviruses are enveloped insect viruses that can carry large quantities of foreign dna in their genome. baculoviruses have proved to be very promising gene therapy vectors but little is known about their transduction mechanisms in mammalian cells. we show in this study that autographa californica multiple nuclear polyhedrosis virus capsid is compatible with the incorporation of desired proteins in large quantities. fusions can be made to the n-terminus or c-terminus of the major capsid protei ...	2003	14599820
baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination.	recombinant baculoviruses derived from the autographa californica nuclear polyhedrosis virus (acnpv) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system (bevs) is hampered by slow and tedious procedures for the selection and separation of baculovirus-infected insect cells and for titer determination. here we developed a new technology based on the bicistronic vector with a fusion protein of the human integral plasma membrane glyc ...	2004	14740489
[cooperative effect of spodoptera litura nuclear polyhedrosis virus with parasite wasp, microplitis sp. on the s. litura larvae].	the relationship between the survival rate, developmental duration of microplitis sp. in spodoptera litura larvae and time of exposure of the host larvae to virus, the inoculation virus concentration were investigated to determined if parasites which emerged from virus-infected s. litura larvae had normal survive, and if the parasites could sever as the vector for s. litura nuclear polyhedrosis virus (sinpv). there was no significant difference in time that parasites spent in virus-infected larv ...	2004	15146645
characterization of two autographa californica nucleopolyhedrovirus proteins, ac145 and ac150, which affect oral infectivity in a host-dependent manner.	the genome of the baculovirus autographa californica nuclear polyhedrosis virus (acmnpv) contains two homologues, orf145 and orf150, of the heliothis armigera entomopoxvirus (haepv) 11,000-kda gene. polyclonal antibodies raised against the ac145 or ac150 protein were utilized to demonstrate that they are expressed from late to very late times of infection and are within the nuclei of infected sf-21 cells. transmission electron microscopy coupled with immunogold labeling of ac145 found this prote ...	2004	15163737
an additional copy of the homologous region (hr1) sequence in the autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes.	the autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. our results show that the 750 bp hr1 sequence is capable of functioning as an enhancer of transcription of foreign genes from the homologous late polyhderin gene promoter and the heterologous drosophila heat shock protein (hsp70) promoter ...	2004	15209510
[expression of human follicle stimulating hormone in insect cells].	to study the expression of hfsh in insect cells, the cdna encoding the hfsh beta chain was cloned by overlapping-pcr using human chromosome dna extracted from placental tissue as template. then we constructed expression vector pvl1393/hfsh beta using an unfused protein nuclear polyhedrosis virus (acnpv) expression vector. the insect cells (sf9) were cotransfected with the expression vector and nuclear polyhedrosis linearized virus dna, and recombinant viruses acnpv-hfsh beta were collected. the ...	2004	15259986
baculovirus late expression factors.	autographa californica nuclear polyhedrosis virus, or acmnpv, is the type member of the baculoviruses, a family of double-stranded dna viruses with large circular genomes. the successive and concomitant expression of an assortment of early, late and very late genes is instrumental for successful baculovirus infection, and requires a switch from early dependence on a host cell-derived polymerase ii to a novel virus-encoded rna polymerase that is required for transcription later on in infection. a ...	2004	15263813
rapid parallel expression in e. coli and insect cells: analysis of five lef gene products of the autographa californica multiple nuclear polyhedrosis virus (acmnpv).	a number of strategies are emerging for the high throughput (htp) expression of recombinant proteins to enable structural and functional study. here we describe a workable htp strategy based on parallel protein expression in e. coli and insect cells. using this system we provide comparative expression data for five proteins derived from the autographa californica polyhedrosis virus genome that vary in amino acid composition and in molecular weight. although the proteins are part of a set of fact ...	2004	15284479
characterization of lactate dehydrogenase-elevating virus orf6 protein expressed by recombinant baculoviruses.	lactate dehydrogenase-elevating virus (ldv) has a strict species-specificity and can replicate only in a subset of mouse primary macrophages in vitro. because it is difficult to grow and purify sufficient quantities of ldv virions from the primary macrophages, it has been difficult to further characterize ldv envelope proteins. a few expression systems have been reported for structural analysis of the nonglycosylated envelope protein m/vp-2, however, very few studies of the antigenicity of m/vp- ...	2004	15325515
ha-vp39 binding to actin and the influence of f-actin on assembly of progeny virions.	we present evidence that actin is necessary for the successful assembly of hanpv virions. purified nucleocapsid protein ha-vp39 of heliothis armigera nuclear polyhedrosis virus (hanpv) was found to be able to bind to actin in vitro without assistance, as demonstrated by western blot and isothermal titration calorimeter. deltah and binding constants (k) detected by isothermal titration calorimeter strongly suggested that ha-vp39 first binds actin to seed the formation of hexamer complex of actin, ...	2004	15503206
promoter and piggybac activities within embryos of the potato tuber moth, phthorimaea operculella, zeller (lepidoptera: gelechiidae).	potato production in tropical and subtropical countries suffers from damage caused by the potato tuber moth (ptm), phthorimaea operculella. the aim of this research was the development of the components required for a germline transformation system for the ptm. we tested three components that are critical to genetic transformation systems for insects: promoter activity, marker gene expression, and transposable element function. we compared the transcriptional activities of five different promote ...	2004	15527988
high-level expression of recombinant 3ab1 non-structural protein from fmdv in insect larvae.	for its potential usefulness in diagnosis, the non-structural protein 3ab1 from foot-and-mouth disease virus was expressed as a soluble protein by using autographa californica nuclear polyhedrosis virus as a vector. the 3ab1 coding sequence was introduced into acnpv genome via pbacpak3ab1 transfer vector to originate ac3ab1 recombinant baculovirus of phenotype occ-. rachiplusia nu larvae were injected with supernatants of sf9 cells infected with ac3ab1 and 5 days post-infection total protein ext ...	2004	15664073
expression and one-step purification of bovine interleukin-21 (il-21) in silkworms using a hybrid baculovirus expression system.	a hybrid baculovirus, a hybrid of the autographa californica nuclear polyhedrosis virus and the bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of bovine interleukin-21 (il-21) in silkworms. a recombinant hybrid baculovirus containing the full length of the cdna of bovine interleukin-21 was constructed and used to infect silkworm larvae or silkmoth pupae. after the infection of the virus, bovine mature il-21 was produced in the haemolymph or pupal cell lysates. a ...	2004	15604780
construction of a host range-expanded hybrid baculovirus of bmnpv and acnpv, and knockout of cysteinase gene for more efficient expression.	acnpv (autographa californica nuclear polyhedrosis virus) and bmnpv(bombyx mori nuclear polyhedrosis virus) are two principal insect-baculovirus expression systems, each having different characteristics. acnpv has a wider host range and can infect a series of cell lines thus making it suitable for cell suspension culture expression, but the small size of the host insect, a. californica, makes acnpv less suitable for large scale protein synthesis. in contrast, bmnpv can only infect the silkworm, ...	2004	15623152
preparation of chil-2 and ibdv vp2 fusion protein by baculovirus expression system.	this study aims to produce an effective subunit vaccine against infectious bursal disease virus (ibdv). the genes of chicken interleukin-2 (chil-2) and ibdv viral protein 2 (vp2) were amplified and fused by splice overlap extension-polymerase chain reaction (soe-pcr). the fusion gene was digested by ecor i/kpn i and inserted into pbacpak8 vector, resulting in recombinant transfer plasmid pbacpakvp2-il2. the recombinant plasmid was transfected into sf-9 cells accompanied with hybrid nuclear polyh ...	2005	16212892
insecticidal effects of buthus occitanus tunetanus botit6 toxin expressed in escherichia coli and baculovirus/insect cells.	botit6 is a neurotoxin polypeptide derived from the venom of the scorpion buthus occitanus tunetanus (bot). its mature form is composed of 62 amino acids. botit6 has been reported to be the most potent toxin from bot venom that has a strict selectivity for insects. such toxin may have potential as a potent animal-harmless tool against insects. using rt-pcr, we isolated and sequenced a cdna encoding 62 amino acid residues corresponding to the known amino acid sequence of botit6. we have expressed ...	2005	16216259
transformed lepidopteran cells expressing a protein of the silkmoth fat body display enhanced susceptibility to baculovirus infection and produce high titers of budded virus in serum-free media.	baculovirus vectors constitute important tools for therapeutic protein production and mammalian cell transduction for gene therapy applications. a prerequisite for such applications is that the cell lines in which baculoviruses are propagated be maintained in serum-free media that are devoid of potential human pathogens. however, in serum-free media, the performance of baculovirus-based systems can be significantly reduced. in this report, we show that silkmoth-derived host cell lines for the bo ...	2005	16233927
development of a new high-throughput screening model for human high density lipoprotein receptor (cla-1) agonists.	to develop a new high-throughput screening model for human high-density lipoprotein (hdl) receptor (cd36 and limpii analogous-1, cla-1) agonists using cla-1-expressing insect cells.	2005	16274109
involvement of the toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus.	we have previously shown that mice inoculated intranasally with a wild-type baculovirus (autographa californica nuclear polyhedrosis virus [acnpv]) are protected from a lethal challenge by influenza virus. however, the precise mechanism of induction of this protective immune response by the acnpv treatment remained unclear. here we show that acnpv activates immune cells via the toll-like receptor 9 (tlr9)/myd88-dependent signaling pathway. the production of inflammatory cytokines was severely re ...	2005	15709004
construction of a transposon-mediated baculovirus vector hanpvid and a new cell line for expressing barnase.	in this study we developed the transposon-mediated shuttle vector 'hanpvid', which composed of hanpv (heliothis armigera nuclear polyhedrosis virus) genomic dna and a transposon cassette from bacmid of bac-to-bac system. hanpvid replicates in e. coli in the same way as bacmid and retains infective function in cotton bollworm cells (hz-am1). using hanpvid we constructed a recombinant virus, which could infect hz-am1 cells and generate recombinant hanpv (rha-bar) containing the barnase gene, a rib ...	2005	15715945
the function of envelope protein p74 from autographa californica multiple nucleopolyhedrovirus in primary infection to host.	this research investigated the function of envelope protein p74 of autographa californica multiple nuclear polyhedrosis virus (acmnpv) in primary infection to host. a p74-inactivation recombinant baculovirus, rac-gfp(delta) p74, was constructed by inserting gfp driven by acmnpv polyhedrin promoter into the p74 locus of acmnpv genome. bioassays showed that the p74-null occlusion bodies (obs) failed to infect its natural host larvae, spodoptera exigua, per os, while the p74-null budded virus (bvs) ...	2005	15744572
cloning and characterization of the g protein betagamma subunits from trichoplusia ni (high five cells).	baculoviral-mediated expression in insect cells has become a method of choice where high-level protein expression is desired and where expression in escherichia coliform (e. coli.) is unsuitable. genes of interest are inserted into the baculoviral genome of autographa californica nuclear polyhedrosis virus (acnpv) under the extremely strong, but very late polyhedron gene (polh). the preferred host lines are derived from spodoptera frugiperda (sf9 or sf21) or tricoplusia ni (high five, invitrogen ...	2005	15763469
microarray and rt-pcr screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp.	here, we report for the first time the successful use of cycloheximide (chx) as an inhibitor to block de novo viral protein synthesis during wssv (white spot syndrome virus) infection. sixty candidate ie (immediate-early) genes were identified using a global analysis microarray technique. rt-pcr showed that the genes corresponding to orf126, orf242 and orf418 in the taiwan isolate were consistently chx-insensitive, and these genes were designated ie1, ie2 and ie3, respectively. the sequences for ...	2005	15780883
baculoviral polyhedrin-bacillus thuringiensis toxin fusion protein: a protein-based bio-insecticide expressed in escherichia coli.	previously, we found that baculoviral polyhedrin (polh) used as a fusion partner for recombinant expression in escherichia coli showed almost the same characteristics (rapid solubilization under alkaline conditions and specific degradation by specific alkaline proteases in insect midgut) as the native baculoviral polh, and formed easily isolatable inclusion bodies. here, polh derived from the autographa californica nuclear polyhedrosis virus (acnpv) was fused with a bacillus thuringiensis (bt) t ...	2005	15981278
fossil evidence of insect pathogens.	the present report describes fossil evidence of insect pathogens, heretofore, almost non-existent, from six samples of amber ranging in age from 15 to 100 million years. they include a cytoplasmic polyhedrosis virus and trypanosomatid infection in an adult biting midge (diptera: ceratopogonidae), and a nuclear polyhedrosis virus in an adult sand fly (diptera: phlebotomidae), both from early cretaceous burmese amber, several types of fungal thalli on the cuticle of an adult mosquito (culicidae: d ...	2005	16009374
genetically engineered horseradish peroxidase for facilitated purification from baculovirus cultures by cation-exchange chromatography.	an engineered horseradish peroxidase isozyme c (hrp c) gene was constructed by the addition of a 6xarg fusion tail to 6xhis-hrp c by the pcr strategy. the 6xhis-6xarg-hrp c cdna was expressed in the sf9 insect cell line from spodoptera frugiperda infected with autographa californica nuclear polyhedrosis virus. the recombinant peroxidase isoelectric point was 9.5 as judged by isoelectric focusing and was purified directly from the culture medium at day-6 post-infection by cation-exchange chromato ...	2005	16038999
from metchnikoff to monsanto and beyond: the path of microbial control.	in 125 years since metchnikoff proposed the use of metarhizium anisopliae to control the wheat cockchafer and brought about the first field trials, microbial control has progressed from the application of naturalists' observations to biotechnology and precision delivery. this review highlights major milestones in its evolution and presents a perspective on its current direction. fungal pathogens, the most eye-catching agents, dominated the early period, but major mycological control efforts for ...	2005	16039302
efficient large-scale protein production of larvae and pupae of silkworm by bombyx mori nuclear polyhedrosis virus bacmid system.	silkworm is one of the most attractive hosts for large-scale production of eukaryotic proteins as well as recombinant baculoviruses for gene transfer to mammalian cells. the bacmid system of autographa californica nuclear polyhedrosis virus (acnpv) has already been established and widely used. however, the acnpv does not have a potential to infect silkworm. we developed the first practical bombyx mori nuclear polyhedrosis virus bacmid system directly applicable for the protein expression of silk ...	2005	15596136
the murine thymic stromal lymphopoietin receptor is partially expressed with an extracellular c-terminus.	the gene encoding the murine thymic stromal lymphopoietin receptor was expressed in lepidopteran insect cells using the baculovirus expression vector system. the corresponding gene was inserted under the polyhedrin promoter of the autographa californica nuclear polyhedrosis virus and expressed with an n-terminal poly-histidine tag and a c-terminal flag-tag in the spodoptera frugiperda insect cell line sf9 during viral infection. flow cytometer analysis of cells infected with the produced recombi ...	2005	15488943
loss of posterior silk gland transcription specificity of fibroin light chain promoter due to absence of 41 bp sequence containing possible inhibitor binding sites.	the gene encoding fibroin light chain protein (fibl) is specifically expressed in the posterior silk gland of silkworm and repressed in other tissues. the binding sites of several transcription factors involved in the silk gland transcription specificity of fibl promoter have been recognized, including sgfb, psgf and bmfa. here we report the leak expression of the enhanced green fluorescent protein (egfp) reporter gene in tissues other than the posterior silk gland in vivo when under the control ...	2005	16331326
characterization of baculovirus autographa californica multiple nuclear polyhedrosis virus infection in mammalian cells.	the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) is used as a vector in many gene therapy studies. wild-type acmnpv infects many mammalian cell types in vitro, but does not replicate. we investigated the dynamics of acmnpv genomic dna in infected mammalian cells and used flow cytometric analysis to demonstrate that recombinant baculovirus containing a cytomegalovirus immediate early promoter/enhancer with green fluorescent protein (gfp) expressed high levels of ...	2006	16545777
a novel method for in vitro radiolabeling and testing enveloped viruses by phosphatidylethanolamine n-methyltransferase and host cell-specific binding.	the present study developed a novel virus labeling and testing method, referred to as an envelope-labeled virus assay (elva), in which virus envelope is labeled in vitro by the action of phosphatidylethanolamine n-methyltransferase (pemt) and tested through a host cell-specific binding. a recombinant strain (vgfpuv) of autographa californica multiple nuclear polyhedrosis virus (acmnpv) and spodoptera frugiperda (sf-9) insect cells were used as a model of viruses and host cells, respectively. the ...	2006	16552776
expression of bioactive recombinant bovine interferon-gamma using baculovirus.	the precise role of bovine interferon-gamma (boifn-gamma) in disease and therapy is still poorly defined. clearly it is involved in defence against parasites, bacteria, viruses and possibly tumor cells. this paper reports the expression of boifn-gamma in a baculovirus system to generate a fully functional recombinant protein. bovine interferon-gamma cdna was cloned from mitogen stimulated peripheral blood mononuclear cell (pbmc) rna utilizing the reverse transcription-polymerase chain reaction ( ...	2006	16608121
enhancing insecticidal efficacy of baculovirus by early expressing an insect neurotoxin, lqhit2, in infected trichoplusia ni larvae.	lqhit(2), an insect specific neurotoxin from the venom of leiurus quinquestriatus hebraeus, has been demonstrated to improve insecticidal efficacy of autographa californica nuclear polyhedrosis virus (acmnpv). a polyhedrin-positive recombinant acmnpvvacp(hsp70)egfp/p(pag90)it(2) was engineered for larvae to express the enhanced green fluorescence protein (egfp) and lqhit(2) under the control of p(hsp70) and p(pag90) promoters, respectively. this would allow a visual observation of the viral infe ...	2006	16636828
cross infectivity of baculovirus, spilarctia obliqua nuclear polyhedrosis virus against mulberry pest, porthesia xanthorrhoea kollar.	spilarctia obliqua nucleopolyhedrosis virus (sonpv) has been found effective against s. obliqua (walker) as well as porthesia xanthorrhoea kollar. lc50 concentration of s. obliqua sonpv against s. obliqua and p. xanthorrhoea was 2.5 x 10(4) and 3.7 x 10(4) pobs/ml, respectively. lt50 of sonpv against s. obliqua was 5.73 days, while it was 6.98 days for p. xanthorrhoea. cross infectivity of sonpv against p. xanthorrhoea and ease of its mass production may make it an ideal biopesticide.	2006	16708898
improving baculovirus transduction of mammalian cells by surface display of a rgd-motif.	an rgd-containing peptide, comprising 23 amino acids from the foot-and-mouth disease virus (fmdv) vp1 protein was engineered into the envelope of autographa californica nuclear polyhedrosis virus surface (acnpv) using two different display strategies. the rgd-motif is a well-described tripeptide, that by binding to cell surface integrins facilitates virus entry into cells. this epitope was displayed, either by directly modifying the native major envelope protein gp64 of acnpv, or by incorporatin ...	2006	16716431
effects of cry1ac toxin of bacillus thuringiensis and nuclear polyhedrosis virus of helicoverpa armigera (hübner) (lepidoptera: noctuidae) on larval mortality and pupation.	in the laboratory, the percentage mortality and pupation of helicoverpa armigera (hübner) were investigated when larvae were exposed to cry1ac of bacillus thuringiensis berliner, nuclear polyhedrosis virus of h. armigera (hanpv) or cry1ac and hanpv together. the results revealed that interactions between cry1ac and hanpv varied with bioassay method and concentration of the suspension. when larvae were infected using a suspension containing both hanpv and cry1ac, most combinations of cry1ac (62.5 ...	2006	16770833
cloning and characterization of bombyx mori pp-bp a gene induced by viral infection.	the enf peptide family, so termed after the consensus sequence in their amino termini (glu-asn-phe-), is assumed to play multiple important roles in defense reactions, growth regulation, and homeostasis of lepidopteran insects. the paralytic peptide of bombyx mori (bmpp) is one such peptide that is involved in the paralytic and plasmatocyte-spreading activities in the hemocyte immune reaction. the growth-blocking peptide of pseudaletia separata (psgbp), which is also a member of the enf peptide ...	2006	17112968
optimization of baculovirus transduction on freestyle293 cells for the generation of influenza b/lee/40.	recombinant baculovirus expression vectors derived from the autographa californica nuclear polyhedrosis virus can serve as efficient gene-transfer vehicles for transient expression of recombinant proteins in a wide range of mammalian cell types and are able to produce multisubunit particles such as viruses or virus like particles. in this study, we constructed eight recombinant baculoviruses each containing one of the influenza b/lee/40 virus genes in a bidirectional expression cassette for simu ...	2006	17172661
[improved efficient gene transfer into insect and mammalian cells by baculovirus vectors].	the fragments of genomics dna of the nuclear polyhedrosis virus (npv) containing genes of late viral proteins p10, p35, p39, were cloned, the promoter regions of this genes were used to design baculovirus transfer vectors. a double-promoter and triple-promoter baculovirus transfer vectors were obtained. recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus (cmv) promoter, the gene for green or red fluorescent protein, sv40pa and polylinker mcs were constru ...	2006	17209436
the autographa californica nuclear polyhedrosis virus acnpv induces functional maturation of human monocyte-derived dendritic cells.	the initiation of an adaptive immune response is critically dependent on the activation of dendritic cells (dcs). therefore, vaccination strategies targeting dcs have to ensure a proper presentation of the immunogen as well as an activation of dcs to accomplish their full maturation. viral vectors can achieve gene delivery and a subsequent presentation of the expressed immunogen, however, the immunization efficiency may be hampered by an inhibition of dc activation. here we report that the insec ...	2006	16870312
expression of spider flagelliform silk protein in bombyx mori cell line by a novel bac-to-bac/bmnpv baculovirus expression system.	bombyx mori nuclear polyhedrosis virus (bmnpv) baculovirus expression system (bes) has a lot of advantages such as high expression efficiency, convenience, and low feeding cost. in this report, we used a recently developed bmnpv bacmid, which could infect both b. mori cell lines and silkworm larvae. the results showed it takes only 7 to 10 days to generate recombinant baculovirus and permit the rapid isolation from small-scale cultures and then use it to transfect b. mori cell lines, compared to ...	2006	16158284
the use of a recombinant baculovirus expressing a chitinase from the hard tick haemaphysalis longicornis and its potential application as a bioacaricide for tick control.	baculoviruses are specific insect pathogens used as selective biological insecticides on lepidopteran insects. we have tested a recombinant baculovirus expressing a chitinase gene for its efficacy as a tick bioacaricide. the recombinant autographa californica multiple nuclear polyhedrosis virus expressing a chitinase enzyme (acmnpv-cht1) from the hard tick, haemaphysalis longicornis, was constructed and found to have a novel bioacaricidal effect against ticks. the recombinant baculovirus was use ...	2006	16292677
functional expression of lepidopteran-selective neurotoxin in baculovirus: potential for effective pest management.	recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. recently we identified and characterized a novel lepidopteran-selective toxin, buthus tamulus insect-selective toxin (butait), having 37 amino acids and eight half cysteine residues from the venom of the south indian red scorpion, mesobuthus tamulus. the synthetic toxin gene containing the butait se ...	2006	16406338
in vivo analysis of fibroin heavy chain signal peptide of silkworm bombyx mori using recombinant baculovirus as vector.	in order to investigate the functional signal peptide of silkworm fibroin heavy chain (fibh) and the effect of n- and c-terminal parts of fibh on the secretion of fibh in vivo, n- and c-terminal segments of fibh gene were fused with enhanced green fluorescent protein (egfp) gene. the fused gene was then introduced into silkworm larvae and expressed in silk gland using recombinant acmnpv (autographa californica multiple nuclear polyhedrosis virus) as vector. the fluorescence of egfp was observed ...	2006	16466694
antibody-based surface plasmon resonance detection of intact viral pathogen.	surface plasmon resonance (spr) technique was used to directly detect an intact form of insect pathogen: the baculovirus, autographa californica multiple nuclear polyhedrosis virus (acmnpv). an spr sensor chip with three bio-functional layers was used to detect the intact acmnpv: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, protein a, and a mouse igg monoclonal antibody raised against a surface protein of the target viral pathogen. a two-channel (reference & tes ...	2006	16470580
construction of hybrib autographa californica nuclear polyhedrosis bacmid by modification of p143 helicase.	we developed a new hybrid nuclear polyhedrosis virus (npv) bacmid capable of infecting spodoptera frugiperda, tricoplusia ni, and bombyx mori, and b. mori cell lines for producing hybrid recombinant baculovirus that can carry a gene of interest and express it in a broad range of hosts. a gfp(uv)-beta1,3-n-acetylglucosaminyltransferase 2 fusion gene was expressed successfully in silkworm larvae using this hybrid bacmid. the hybrid npv bacmid provides an altogether simple and realistically feasibl ...	2006	16478634
baculovirus etl promoter acts as a shuttle promoter between insect cells and mammalian cells.	to identify a shuttle promoter that can mediate gene expression in both insect cells and mammalian cells to facilitate the development of a baculovirus vector-based mammalian cell gene delivery vehicle.	2006	16490168
function and oligomerization study of the leucine zipper-like domain in p13 from leucania separata multiple nuclear polyhedrosis virus.	the p13 gene is uniquely present in group ii nucleopolyhedroviruses (npvs) and some granuloviruses, but not in group i npvs. p13 gene was first described by our laboratory in leucania separatamultiple nuclear polyhedrosis virus (ls-p13) in 1995. however, the functions of ls-p13 and of its homologues are unknown. when ls-p13 was inserted into autographa californica nucleopolyhedrovirus, a group i npv, polyhedra yield was inhibited. however, this inhibition was prevented when the leucine zipper-li ...	2007	17394774
a highly efficient method for the generation of a recombinant bombyx mori nuclear-polyhedrosis-virus bacmid and large-scale expression of foreign proteins in silkworm (b. mori) larvae.	in the post-genomic era, one of the challenges and a source of competition is the development of high-throughput, large-scale and low-cost eukaryotic cdna cloning and expression systems. the baculovirus expression system is the most popular one and plays an important role in the high-level expression of eukaryotic proteins. in the present study, a convenient, rapid and highly efficient method for the construction of recombinant bmnpv (bombyx mori nuclear polyhedrosis virus)-bacmid vector (bmbacm ...	2007	17428194
influence of cytochrome c on apoptosis induced by anagrapha (syngrapha) falcifera multiple nuclear polyhedrosis virus (afmnpv) in insect spodoptera litura cells.	we investigated the influence of cytochrome c on apoptosis induced by anagrapha (syngrapha) falcifera multiple nuclear polyhedrosis virus (afmnpv). microscopic observation revealed that infection of sl-1 cells with afmnpv resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of afmnpv-infected sl-1cells. western blot analysis demonstrated that afmnpv-induced apoptosis in insect sl-1 cells was significantly inhibited by cyclosporin a which blocked a translocation of cyt ...	2007	17478109
purification and characterization of a recombinant rat prohaptoglobin expressed in baculovirus-infected sf9 insect cells.	to generate hemoglobin-free full-length haptoglobin the cdna encoding rat haptoglobin alphabeta subunits was cloned into shuttle vector pvt-bac-his and used to produce a recombinant baculovirus autographa californica nuclear polyhedrosis virus (acnpv) as an expression vector, named hpacnpv. recombinant virus was used to infect spodoptera frugiperda (sf9) insect cells. the 50 kda protein expressed was mostly secreted into the culture medium at relatively high titer (15 microg/ml) and was found to ...	2007	17681809
p13 of leucania separata multiple nuclear polyhedrosis virus affected the polyhedra and budded virions yields of acmnpv.	p13 gene was first described by our laboratory in leucania separata multiple nuclear polyhedrovirus (ls-p13, orf114) back to 1995. however, the functions of ls-p13 and its reported homologues remained unknown. in order to probe the function of ls-p13, recombinant autographa californica nucleopolyhedroviruses (racmnpvs) were constructed to express ls-p13 in the sf9 cells at early, late or early/late phase. observations of microscope showed that the expression of ls-p13 could decrease the yield of ...	2007	17141348
enhanced effect of fluorescent whitening agent on peroral infection for recombinant baculovirus in the host bombyx mori l.	the low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (bombyx mori l). in this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. the results showed that the peroral infection can be remarkably enhanced by adding ...	2007	17160363
cloning and expression analysis of heat shock cognate 70 gene promoter in tiger shrimp (penaeus monodon).	heat shock cognate 70 (hsc70) functions as a molecular chaperon and plays an important role in protein folding. hsc70 cdna of tiger shrimp (penaeus monodon) was cloned and characterized in our previous study. after shrimps were treated with the 1-hr heat shock, the hsc70 mrna level in hemocytes increased (approximately 8 fold) using real-time quantitative pcr. an hsc70 clone was obtained from genomic library screening. the gene contains 2 exons separated by a 1557-bp intron. the 5'-flanking regi ...	2007	17931801
using cell size kinetics to determine optimal harvest time for spodoptera frugiperda and trichoplusia ni bti-tn-5b1-4 cells infected with a baculovirus expression vector system expressing enhanced green fluorescent protein.	infecting insect cells with a baculovirus expression vector system (bevs) is an increasingly popular method for the production of recombinant proteins. due to the lytic nature of the system, however, determining the optimal harvest time is critical for maximizing protein yield. we found that measuring the change in average diameter during the progress of infection with an automated cell analysis system (cedex hires, innovatis ag) could be used to determine the time of maximum protein production ...	2007	19003016
induction of antitumor acquired immunity by baculovirus autographa californica multiple nuclear polyhedrosis virus infection in mice.	the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) has been studied as a gene therapy vector. here, we demonstrated that acmnpv induces antitumor acquired immunity. these results suggest that acmnpv has the potential to be an efficient virus or tumor therapy agent which induces innate and acquired immunity.	2008	18057182
induction of natural killer cell-dependent antitumor immunity by the autographa californica multiple nuclear polyhedrosis virus.	wild-type autographa californica multiple nuclear polyhedrosis virus (acmnpv) infects a variety of mammalian cell types in vitro, but does not replicate in these cells. we investigated the effects of acmnpv in the induction of the immune response and tumor metastasis in mice. after intravenous injection, acmnpv was taken up by the liver and spleen, and preferentially infected dendritic cells (dcs) and b cells in the spleen; costimulatory molecules cd40, cd80, and cd86 were upregulated in the dcs ...	2008	18059370
the effects of bmnpv on biochemical changes in primary cultures of bombyx mori embryonic tissue.	the effect of bombyx mori nuclear polyhedrosis virus (bmnpv) on biochemical changes of tc-100 medium containing 10% fetal bovine serum (fbs) in embryonic primary cultures of silkworm was investigated. the primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums (diluted with tc-100 medium representing multiplicity of infection (moi) of 0.25, 0.5, and 1). glucose, uric acid, urea, total protein, cholesterol, and alkaline phosphatase were measured in the mediu ...	2008	18288542
acmnpv ac143 (odv-e18) is essential for mediating budded virus production and is the 30th baculovirus core gene.	autographa californica multiple nucleopolyhedrovirus (acmnpv) ac143 (odv-e18) is a late gene that encodes for a predicted 9.6 kda structural protein that locates to the occlusion derived viral envelope and viral induced intranuclear microvesicles [braunagel, s.c., he, h., ramamurthy, p., and summers, m.d. (1996). transcription, translation, and cellular localization of three autographa californica nuclear polyhedrosis virus structural proteins: odv-e18, odv-e35, and odv-ec27. virology 222, 100-1 ...	2008	18328526