Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| epstein-barr virus and nonhuman primates: natural and experimental infection. | 1976 | 179290 | |
| growth in semisolid agar medium of human cord leukocytes freshly transformed by epstein-barr virus. | the semisolid agar method for the selective growth of transformed cells was applied to investigations of epstein-barr virus (ebv) transformation. when 1 x 10(5) human umbilical-cord blood leukocytes were seeded in agar medium immediately after ebv exposure, about 100 colonies developed in each dish. the occurrence of colonies correlated well with dilutions of ebv inoculum. these colonies were composed of lymphoblasts, were positive for ebv-determined nuclear antigen immunofluorescence, and cons ... | 1976 | 176377 |
| workshop on epstein-barr virus: summary and recommendations. | 1976 | 176391 | |
| antibody-dependent lymphocyte cytotoxicity against cells expressing epstein-barr virus antigens. | antibody-dependent lymphocyte cytotoxicity (adlc) was demonstrated against epstein-barr virus (ebv)-infected raji cells with peripheral with peripheral blood lymphocytes from ebv-infected donors. no cytotoxic activity was detected against unifected rajl cells. the results indicated that this antibody-mediated cytotoxic reaction was directed against the same ebv-induced membrane antigens (ma) previously defined by the membrane immunofluorescence (mf) assay. antibody to ebv-associated early antige ... | 1976 | 176393 |
| epstein-barr virus--is it time to develop a vaccine program? | 1976 | 176406 | |
| epstein-barr virus in an american patient with burkitt's lymphoma: detection of viral genome in tumor tissue and establishment of a tumor-derived cell line (nab). | epstein-barr virus (ebv) dna (17.7 genome equivalents/cell) was found in tumor tissue from an american patient with burkitt's lymphoma who had never traveled outside the united states. a lymphoid cell line (nab) containing the ebv genome was established from tumor tissue from this patient; characteristics of this cell line were described. previous burkitt's tumors found in americans and examined by molecular hybridization were negative for ebv dna. our results suggested that ebv is associated wi ... | 1976 | 176407 |
| characterization of epstein-barr virus antigens. i. biochemical analysis of the complement-fixing soluble antigen and relationship with epstein-barr virus-associated nuclear antigen. | the epstein-barr virus-soluble (s) antigen extracted from raji cells was characterized by sucrose gradient centrifugation, gel filtration, and ion-exchange chromatography. the sedimentation coefficient was estimated to be 8.5s corresponding to a molecular weight of 180,000. the s antigen binds to deae-a25 ion exchanger from which it can be eluted with 0.3 m nacl in tris buffer (ph 7.2). all fractions which contained complement-fixing s antigen also inhibited the anticomplement immunofluorescence ... | 1976 | 176436 |
| proteins of epstein-barr virus. i. analysis of the polypeptides of purified enveloped epstein-barr virus. | epstein-barr virus (ebv) was purified from the extracellular fluid of hr-1 and b95-8 cell lines. the preparations of purified virus consisted of enveloped particles and had ebv-specific antigneic reactivity. comparison of the amount of labeled protein in preparations of virus purified from cultures incubated in [35s]methionine with the amount of labeled protein in preparations obtained following a mixture of unlabeled virus with [35s]methionine-labeled cellular proteins indicated that less than ... | 1976 | 176452 |
| inhibition of epstein-barr virus dna synthesis and late gene expression by phosphonoacetic acid. | growth of lymphoblastoid cells (b95-8, raji) is not inhibited by the presence of 0.4 mm phosphonoacetic acid. the synthesis of epstein-barr virus (ebv) in the producer line b95-8 is completely inhibited, as shown by the total inhibition of viral capsid antigen synthesis. early viral antigens are made normally in the presence of phosphonoacetic acid, but ebv dna synthesis is blocked in cells entering the productive cycle. nonproducer cells in the population replicate the resident ebv dna by a mec ... | 1976 | 176457 |
| proteins of epstein-barr virus. ii. electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine- and polysaccharide-containing components of enveloped virus. | two series of experiments were undertaken to identify the topological location of the structural polypeptides of epstein-barr virus. in the first series of experiments, nucleocapsids prepared by detergent treatment of enveloped virus with nonidet p-40 and sodium deoxycholate were found to be composed of seven polypeptides, vp2, 6, 7.5, 24, 27, 31, and 33, which ranged in molecular weight from over 200 x 10(3) to 28 x 10(3). nine other polypeptides, vp 4, 7, 8, 10, 15, 16, 23, 28, and 29, could b ... | 1976 | 176465 |
| intracellular forms of epstein-barr virus dna in human tumour cells in vivo. | tumour biopsies from burkitt lymphoma patients, as well as human nasopharyngeal carcinoma cells growing in athymic mice, contain epstein-barr virus dna as covalently closed circular dna. in addition integrated viral dna sequences seem to be present. | 1976 | 176597 |
| association of epstein-barr viral genomes with american burkitt lymphoma. | 1976 | 176599 | |
| effect of interferon on lymphocyte transformation and nuclear antigen production by epstein-barr virus. | 1976 | 176601 | |
| superinfection epithelial nasopharyngeal carcinoma cells with epstein-barr virus. | attempts were made to superinfect epithelial explant cell cultures prepared from nasopharyngeal carcinomas with epstein-barr virus. virus-specific markers were observed in such cultures 3 days after superinfection. in addition, expression of epstein-barr virus early antigens was observed in epithelial cell explant cultures treated with iododeoxyuridine. the results suggest that epithelial cells of at least certain nasopharyngeal carcinomas possess the receptor for the epstein-barr virus and that ... | 1976 | 176664 |
| approaches to immunological control of virus-associated tumors in man: introductory remarks. | 1976 | 175916 | |
| epstein-barr virus-malaria interaction models for burkitt's lymphoma: implications for preventive trials. | infections with both epstein-barr virus (ebv) and malaria have been implicated as causal factors in the pathogenesis of burkitt's lymphoma (bl). proposed trials of preventive measures for both infections are receiving serious consideration as possible means of establishing a causal relationship with bl. in this paper we examine certain models for the interaction of ebv and malaria in the induction of bl, and also review the aims of the longitudinal, population-based study being conducted in the ... | 1976 | 175923 |
| experimental models for dna-rna viral interactions: a brief review. | evidences that demonstrate or imply the occurrence of dna-rna viral interactions in man and animals are reviewed. the concurrent presence of two dissimilar oncogenic viruses influenced responses both in vivo and in vitro. cell-mediated immune responses to marek's disease herpesvirus were altered in specific-pathogen-free chickens inoculated as embryos with an avian leukosis virus. the possible roles of immunological factors in the pathogenesis and control of tumor development associated with dna ... | 1976 | 175924 |
| biochemical approaches to detection of epstein-barr virus in human tumors. | the application of biochemical studies for the detection of epstein-barr virus (ebv)-dna in human tumor cells is discussed. these studies resulted in the consistent demonstration of viral nucleic acid in african burkitt's lymphoma biopsies and in epithelial tumor cells of nasopharyngeal carcinomas. the viral dna resides within those cells regularly in multiple copies per cell. besides these tumors our group detected significant concentrations of ebv-dna in a german lymphoma patient revealing his ... | 1976 | 175925 |
| implications of a vaccine for the prevention of epstein-barr virus infection: ethical and logistic considerations. | reasons are given for considering that there is sufficiently substantial indirect and circumstantial evidence linking epstein-barr (eb) virus to african burkitt's lymphoma (bl) and nasopharyngeal carcinoma to call for a dynamic new approach to establish a causal role for the virus in these human cancers. it would seem that the only way to do this would be to develop a vaccine, vaccinate a population at risk in a high-tumor-incidence area, and subsequently follow the population for a consequentia ... | 1976 | 175931 |
| immunological control of virus-associated tumors in man: a perspective. | 1976 | 175948 | |
| cell-killing by epstein-barr virus: analysis by colony inhibition procedure. | the p3hr-1 and b95-8 strains of epstein-barr virus (ebv) were cytocidal for ebv-carrier human cell lines, as revealed by a colony inhibition procedure. the cytocidal activity was proportional to virus dose added. the cell killing was neutralized by anti-ebv antibody-positive but not -negative human sera. when the relative sensitivity to ultraviolet light of ebv activities was examined, the cytocidal actitivy was much more resistant than the viral infectivity as assayed by early antigen-forming a ... | 1976 | 176118 |
| [antibodies against the epstein-barr virus, capsid antigen in children with malignant neoplasms (author's transl)]. | sera from children were tested for antibodies to viral capsid antigens of epstein-barr virus. the method applied was the indirect immunofluorescence test. 25 sera from healthy donors were titrated as controls. in 14 patients with acute lymphocytic leukemia we found low levels, a tiny little girl with chronic myelocytic leukemia (?) and three children.with lymphoproliferative disorders showed highly increased serological reactivities. investigated children with other kinds of tumours did not sero ... | 1976 | 175342 |
| eb virus antibody and infectious mononucleosis in a boarding school for boys. | a study of eb virus antibody was undertaken in a boarding school for boys between 11 and 18 years of age; 35% of boys had ebv antibody when first sampled and a small number acquired antibody each year. some of these boys had had no recorded illness during the period before the first positive sample and some developed classic infectious mononucleosis which was accompainied by a positive heterophil antibody test and detection of eb virus specific igm. in a school of 800 boys there were 13 cases of ... | 1976 | 175098 |
| effect of the host cell on the maintenance and replication of epstein-barr virus. | 1976 | 174288 | |
| epstein-barr virus-specific iga serum antibodies as an outstanding feature of nasopharyngeal carcinoma. | stimulated by a report on elevated iga levels in nasopharyngeal carcinoma (npc), we tested a total of 372 sera from patients with npc, other carcinomas of head and neck or elsewhere, burkitt's lymphoma (bl), infectious mononucleosis (im) or healthy controls. the sera were titrated in indirect immunofluorescence tests for iga antibodies to epstein-barr virus (ebv) capsid antigen (vca) and to the diffuse (d) or restricted (r) components of the ebv-induced early antigen (ea) complex. the results pr ... | 1976 | 175020 |
| comparative studies of epstein-barr virus strains from ghana and the united states. | a high incidence of oropharyngeal excretion of epstein-barr virus (ebv) has been observed in african children with burkitt's lymphoma (bl) (48%) and matched controls (45%). this compares with an incidence of 77% in american patients with infectious mononucleosis (im) and 13% in age-matched controls. cross-neutralization tests between ebv strains derived from bl and im patients and their sera failed to detect differences in the major neutralizing antigenic components. cord-blood lymphocytes trans ... | 1976 | 175023 |
| immunity to antigens associated with a cell line derived from nasopharyngeal cancer (npc) in non-chinese npc patients. | delayed hypersensibility to antigens derived from four lymphoid cell lines was measured in 27 non-chinese patients with nasopharyngeal cancer (npc) and 63 non-npc cancer patients. of the npc patients, 17/27 (63%) had a positive skin test response to antigens derived from hkly-28, a lymphoid cell line which was developed from an npc biopsy. only 10/51 (20%) and 1/13 (8%) patients with solid tumors and hematopoietic malignancies, respectively, had positive skin test responses to hkly-28. positive ... | 1976 | 175025 |
| establishment of ebna-expressing cell lines by infection of epstein-barr virus (ebv)-genome-negative human lymphoma cells with different ebv strains. | cells of two ebna (epstein-barr virus nuclear antigen)-negative human lymphoma cell lines, bjab and ramos, were infected with two strains of epstein-barr virus (ebv). in two different experiments, b95-8 virus-infected bjab cells revealed a gradually increasing number of ebna-positive cells. twenty weeks after infection almost 100% of the cell population expressed this antigen. in contrast, it has not so far been possible to convert ramos cells into an ebna-positive cell line. the initial proport ... | 1976 | 175026 |
| attempts to demonstrate virus-specific sequences in human tumors. iv. eb viral dna in european burkitt lymphoma and immunoblastic lymphadenopathy with excessive plasmacytosis. | various biopsies from different european malignant lymphomas, two biopsies from nasopharyngeal carcinomas, and material from non-neoplastic lymph nodes were assayed for the presence of epstein-barr virus (ebv) dna by nucleic acid hybridization. reassociation kinetics of in vitro-labelled ebv dna were studied in the presence of tumor dna. the lymphomas tested included among others follicular lymphomas, germinocytomas, immunoblastic lymphomas and lymphoplasmacytoid immunocytomas. epstein-barr vira ... | 1976 | 175027 |
| clonal transformation of human leukocytes by epstein-barr virus in soft agar. | the b95-8 strain of epstein-barr virus (ebv) induced colony formation of human umbilical cord-blood leukocytes in soft agar medium. one-hit response relationship between the number of colonies and the virus dose was observed with high dilutions of the virus preparation. however, there was a presumed cell-killing effect with low dilutions of virus. the colonies were similarly induced, but with a lower efficiency, in adult peripheral blood leukocyte cultures infected with the virus. the colony-for ... | 1976 | 175028 |
| [classification of malignant lymphomas of the neck: combined morphological, immunocytological, serological and tissues culture studies (author's transl)]. | 81 untreated malignant lymphomas of the neck were classified morphologically according to the german kiel classification and to the american classification of rappaport and berard and these tumors were typed immunologically as to their t- or b-cell nature. cells from 16 of these patients were subsequently grown in tissue culture for periods up to seven months. tissue culture cells were monitored as to spontaneous variations in the morphologic cell type and to the expression of t- or b-cell surfa ... | 1976 | 136561 |
| ebv-determined nuclear antigen (ebna)-positive cells in the peripheral blood of infectious mononucleosis patients. | after removal of srbc rosette-forming t-cells from the peripheral blood, the residual, largely b-lymphocyte fraction of five infectious mononucleosis patients was found to contain 0.5-2% blast cells, positive for the ebv-determined nuclear antigen (ebna). there was a rough parallelism between the presence of large lymphoblasts in the hematological smear, ebns-positive large blasts in the b-cell fraction and the ability of the t-cell fraction to exert an ebv-specific lymphocytotoxicity on establi ... | 1976 | 55396 |
| persistence of epstein-barr viral nuclear antigen (ebna) in cells entering the eb viral cycle. | it was shown by double immunofluorescence studies that epstein-barr viral nuclear antigen (ebna) was preserved in ebv-infected cells after they had entered the productive viral cycle, as signalled by the appearance of the early antigen (ea)complex. a nuclear component of the ea comples could be clearly distinguished from ebna with regard to antigenic specificity. | 1976 | 55398 |
| lymphocyte transformation induced by autologous cells. iii. lymphoblast-induced lymphocyte to stimulation does not correlate with eb viral antigen expression or immunity. | human mitogen-induced and cell line b lymphoblasts stimulate the proliferation of allogeneic and autologous lymphocytes in culture. the role in thes reaction of eb viral determinants on the stimulating cells and immunity of the lymphocyte donor to the eb virus has been studied. the stimulatory capacity of cultured cell line lymphoblasts is not inhibited by incubating lymphoblasts with antisera to eb viral determinants. cultured cell line b lymphoblasts stimulate as much thymidine incorporation b ... | 1976 | 55437 |
| immunological control of human lymphoma: discussion. | 1976 | 56225 | |
| the absence of b-cell antigen b2 from leukaemia cells and lymphoblastoid cell lines. | 60 leukaemia patients with acute mylogenous leukaemia, chronic myelocytic leukaemia, acute lymphatic leukaemia, and chronic lymphocytic leukaemia were tested for five specificities of the new b-lymphocyte alloantigenic system. two specificities of the first b-cell locus were significantly lower in frequency than in b lymphocytes from 105 controls. b-cell group 1 was found in 18% of leukaemia patients vs. 48% of controls (p less than 0.025) and b-cell group 2 was found in 0% of leukaemia patients ... | 1976 | 59011 |
| somatic cell hybrids between human lymphoma lines. ii. spontaneous and induced patterns of the epstein-barr virus (ebv) cycle. | the regulation of spontaneous, iudr-induced and p3hr-1 virus-induced ea and vca production patterns was studied in two new somatic hybrids between human lymphoma lines. the hybrid 8a was derived from the crossing of the non-producer raji with the spontaneous producer daudi line. the second hybrid, 83, was produced by the fusion of raji with the ebv-genome-negative b-lymphoma line, bjab. the studies suggest the following ebv regulation patterns: (1) the spontaneous production of ea and vca appear ... | 1976 | 59708 |
| surface markers on human b and t lymphocytes. viii. association between complement and epstein-barr virus receptors on human lymphoid cells. | an association has been found between the epstein-barr virus (ebv) and complement (c3d) receptors on human lymphoid cells. the evidence was four-fold: there was a correlation between the expression of these two receptors; inhibition experiments showed that the binding sites probably are close to each other in the cell membrane, although not identical; ebv and complement receptors have been found to co-cap in either order; and lymphoid cell lines lacking complement receptors could not be superinf ... | 1976 | 59946 |
| oncogenic viruses: implications for human diseases (part ii). | 1976 | 60244 | |
| molecular probes for tumor viruses in human cancer. | 1976 | 60297 | |
| [virus etiology of lymphomas and leukemias in man]. | 1976 | 61149 | |
| studies on epstein-barr virus-related antigens. i. indirect single radial immunodiffusion as a useful method for detection and assay of soluble antigen. | a useful method for the detection and assay of epstein-barr virus (ebv)-related soluble antigen has been developed by the application of the indirect single radial immunodiffusion technique which is frequently used for quantitative measurements of immunoglobulins and other soluble proteins. when the extracts of ebv-determined nuclear antigen (ebna)-positive non-producer cells (raji and nc-37) were applied to agar plates containing seropositive human serum, followed by overlay with anti-human igg ... | 1976 | 61945 |
| pathogenesis and phenotypes of an x-linked recessive lymphoproliferative syndrome. | a new x-linked recessive lymphoproliferative syndrome has variable phenotypes: fatal infectious mononucleosis (i.m.), agammaglobulinaemia after i.m., american burkitt's lymphoma, histiocytic lymphoma, immunoblastic sarcoma of b cells, or plasmacytoma. an immunodeficiency to rubeola and the epstein-barr virus probably ensues from the mutant gene. the phenotypes (spectrum of b-cell disorders) have a common inheritance and the aetiology is similar. | 1976 | 62116 |
| subpopulations of human lymphoblastoid cell lines. correlation with the expression of surface receptors and content of epstein-barr virus genome. | forty lymphoblast cell lines derived from normal subjects, patients with infectious mononucleosis, leukemia, and burkitt's lymphoma have been studied for surface receptors including surface ig, complement receptors by the eac rosette and fluorescent (raji cell) techniques, and fc (aggregate) receptor by direct and indirect immunofluorescence. because of the b-cell tropism of the epstein-barr virus (ebv), an effort was made to correlate the expresion of various surface properties of lymphoblastoi ... | 1976 | 62390 |
| inducibility of the epstein-barr virus (ebv) cycle and surface marker properties of ebv-negative lymphoma lines and their in vitro ebv-converted sublines. | two ebv-negative lymphoma lines of human b-cell origin, bjab and ramos, were compared with altogether six of their in vitro ebv-converted, ebna- and ebv-dna-carrying sublines (four of ramos and two of bjab derivation). all converted lines closely resembled the parental line with regard to karyotype and hl-a and b antigen typing. induction of ebv antigens (ea and vca) by p3hr-1 virus superinfection was either similar in the converted and the negative lines, or somewhat increased in certain conver ... | 1976 | 62724 |
| aetiology of nasopharyngeal carcinoma. | 1976 | 63856 | |
| the specificity of heterophil antibodies in patients and healthy donors with no or minimal signs of infectious mononucleosis. | over several years sera were collected from 14 heterophil-positive students or patients who did not fulfill minimal hematologic criteria for infectious mononucleosis (i.m.) the specificity of these heterophil reactions for i.m. was investigated by determining antibodies to epstein-barr virus-determined antigens, i.e., to viral capsid antigens (vca), early antigens (ea), and ebv-associated nuclear antigens (ebna). on the basis of detectable anti-ea and/or the early absence and late emergence of a ... | 1976 | 1126 |
| comparison between two antibody populations in the ebv system: anti-ma versus neutralizing antibody activity. | ebv-neutralizing antibody titers were determined in 11 sera derived from african burkitt lymphoma or nasopharyngeal carcinoma patients and in the corresponding serum fractions retained above xm 100 diaflo membranes after low ph treatment, and after recombination of the retained and passed fractions by neutralization of the acidified samples. they were compared with the corresponding antimembrane antigen (ma) titers in seven of the same sera. while all sera tested showed substantial increase of t ... | 1976 | 2559 |
| an immunofluorescence technique with counterstain on fixed cells for the detection of antibodies to human herpesviruses; antibody patterns in patients with hodgkin's disease and nasopharyngeal carcinoma. | an indirect immunofluorescence (if) test on fixed cells with evans' blue counterstain is described for all four human herpesviruses, i.e., herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv) and epstein-barr virus (ebv). comparison with immunodiffusion (id) for hsv-2 and with id and complement fixation (cf) for vzv and cmv demonstrated the specificity and high sensitivity of the if test. also introduced is a modification of the anti-compl ... | 1976 | 67099 |
| epstein barr virus and nasopharyngeal carcinoma. | 1976 | 73653 | |
| letter: immune mechanisms in infectious mononucleosis. | 1976 | 173991 | |
| surface marker characteristics and epstein-barr virus studies of two established north american burkitt's lymphoma cell lines. | tumor cell lines have been established in continuous culture from two north american burkitt's lymphomas. the su-amb-1 line, derived from a patient with low serum antibody titers to epstein-barr virus (ebv), was devoid of ebv genomes by the reaction for ebv-associated nuclear antigen (ebna), could not be induced to express ebv antigens, and was highly refractory to ebv superinfection. conversely, the su-amb-2 cell line, derived from a patient with "african type" serology, yielded a positive ebna ... | 1976 | 174098 |
| antibodies to epstein-barr viral capsid and early antigens associated with burkitt's lymphoma and lymphoblastic lymphosarcoma in israel. | before and/or after chemotherapy was administered to patients with burkitt's lymphoma (bl) or lymphoblastic lymphosarcoma (lls), their sera and those of matched controls were tested for antibodies to epstein-barr virus (ebv) capsid antigen (vca) and early antigen by the indirect immunofluorescence method. ten of the 16 bl patients were arab children and 8 of the 11 lls patients were jews of asian-african origin. although half the bl patients did not have elevated antibody titers when their disea ... | 1976 | 233958 |
| membrane antigen expression in epstein-barr virus-infected raji cells in the presence of phosphonoacetic acid. | the expression of the epstein-barr virus (ebv)-induced membrane antigen (ma) in raji cells experimentally infected with ebv concentrates was inhibited by phosphonoacetic acid (paa) as determined by membrane immunofluorescence and inhibition of antibody-dependent lymphocyte cytotoxicity. paa was only effective if present during the first 24 hr following virus adsorption, indicating that the synthesis of ma was primarily a late viral gene function requiring viral dna synthesis. | 1977 | 18625491 |
| pathogenesis of infectious mononucleosis. | 1977 | 73962 | |
| epstein-barr virus induces viral nuclear antigen in nasopharyngeal epithelial cells. | 1977 | 73981 | |
| effects of 5-tungsto-2-antimoniate in oncogenic dna and rna virus-cell systems. | 1977 | 74337 | |
| meralgia paraesthetica and epstein-barr virus. | 1977 | 67330 | |
| prospective study of epstein-barr virus antibody tetres and survival in patients with nasopharyngeal carcinoma. | 1977 | 67401 | |
| identification of a purified complement-fixing antigen as the epstein-barr-virus determined nuclear antigen (ebna) by its binding to metaphase chromosomes. | a soluble complement-fixing antigen carried by epstein-barr virus (ebv)-transformed human cells has been previously extracted from cell nuclei and purified by dna-cellulose chromatography [luka, j., siegert, w. & klein, g. (1977) j. virol., in press]. on addition of this antigen to methanol/acetic acid-fixed metaphase chrmosomes, followed by exposure to human sera containing antibodies against the ebv-determined nuclear antigen (ebna), brilliant positive staining was obtained by anti-complement ... | 1977 | 67603 |
| quantitation of beta2-microglobulin and hla on the surface of human cells. ii. in vitro cell lines and their hybrids. | beta2-microglobulin (beta2m) participates as an integral part in molecules of the major histocompatibility complex (mhc) type. absence of beta2m makes the residual heavy mhc chain largely inactive as antigen. striking reductions in the density per unit surface area of beta2m in seven out of nine malignant lymphoid tumour lines in comparison with normal lymphocytes or "immortalized" epstein-barr-virus-transformed lymphoblastoid lines were found is this study. this would seemingly represent a spec ... | 1977 | 67633 |
| characterization of ebv-genome negative "null" and "t" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-hodgkin lymphoma. | sixty-two explants from peripheral blood, bone marrow and cerebral fluid of children with acute lymphoblastic leukemia (all) and leukemic transformed non-hodgkin lymphoma (nhl) were cultivated for at least 8 weeks. although lymphatic cells persisted up to 16 weeks in tissue culture, no proliferation was observed in 54 cultures. from the remaining cultures, eight permanently growing cell lines were obtained. five of these were ebna (epstein-barr virus-specific nuclear antigen)-positive. three, ho ... | 1977 | 68013 |
| unusual prevalence of antibodies to epstein-barr virus early antigen in ataxia telangiectasia. | 1977 | 68263 | |
| antibody-dependent cellular cytotoxicity (adcc) against epstein-barr virus-determined antigens. iii. reactivity in sera from patients with burkitt's lymphoma in relation to tumour development. | sera from patients with burkitt's lymphoma (bl) were tested for antibody-dependent cellular cytotoxicity (adcc) against epstein-barr virus (ebv) determined antigens and for antibodies directed against various ebv-specific antigens. no correlation was found between ebv serology and adcc in sera selected for high or low titres against the membrane antigens (ma) or in consecutive sera from three patients with isolated late tumour recurrences. furthermore, no correlation was found between adcc and t ... | 1977 | 70292 |
| cytomegalovirus-directed lymphocyte reactivity in healthy adults tested by a cmv-induced lymphocyte transformation test. | a cytomegalovirus (cmv) induced lymphocyte tranformation test (ltt) performed as a microtechnique assay is described. investigations were done in a group of twenty-five healthy adults with normal lymphocyte reactivity to phytohaemagglutinin and previously encountered antigens. lymphocyte reactivity was established in cmv-seropositive persons using heat-inactivated cell-free cmv strain ad 169 in an optimal stimulating dose of 10(1-5) tcid 50. positive cmv-ltt results were shown to be induced by p ... | 1977 | 70294 |
| terminal deoxynucleotidyl transferase activity and cell surface antigens of two unique cell lines (nalm-1 and balm-2) of human leukemic origin. | two unique cell lines, nalm-1 and balm-2 derived from lymphoblast-like cells of chronic myelogenous leukemia and rare b cell acute lymphoblastic leukemia patients, respectively, were compared with fresh parent cells from the patients and with a philadelphia chromosome positive k-562 cell line previously established from a chronic myelogenous leukemia patient in blastic phase. nalm-1 resembled the parent cells in the presence of philadelphia chromosome, non-t/non-b acute lymphoblastic leukemia sp ... | 1977 | 70413 |
| observations on the antigenic relationships between epstein-barr virus and herpesvirus saimiri. | the antigenic relationships between epstein-barr (eb) virus and herpesvirus saimiri (hvs) have been investigated in comparative immunofluorescence, microimmunodiffusion and serum neutralization tests. no similarity was detected between the structural antigens of the two herpesviruses but the results of microimmunodiffusion tests showed that they shared a non-structural, virus-determined antigen. the implications of this finding are discussed in relation to the importance of hvs and its monkey ly ... | 1977 | 70507 |
| continuous lymphoblastoid suspension cultures from cells of haematopoietic organs of baboons with malignant lymphoma. report iii: immunological studies. | lymphoblastoid cultures established from haematopoietic organs of baboons with lymphoma belong to the b-cell type. cytoplasmic antigen is revealed in 2--6% of suspension culture cells established from lymphomatous baboons by indirect immunofluorescence reaction using serum from human burkitt's lymphoma patients with antibody titer to capsid antigen of epstein-barr virus 1: 320. there is a correlation between the number of antigen-containing cells in these cultures and the number of cells in whic ... | 1977 | 71245 |
| characterization of lymphoid cell lines established from multiple epstein-barr virus (ebv)-induced lymphomas in a cotton-topped marmoset. | 1977 | 71273 | |
| multiple-sclerosis-associated agent. | 1977 | 71613 | |
| [virus and cancer]. | 1977 | 71664 | |
| detection of epstein-barr virus antigens with enzyme-conjugated antibody. | monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ... | 1977 | 72045 |
| human b cell line deficient in the expression of b cell-specific glycoproteins (gp 27,35). | a human b lymphoid cell line, p3hr-1, expresses only low levels of the 27 000 and 35 000 mol.wt. b cell-specific glycoproteins (gp 27,35). indirect antibody-binding and quantitative absorption tests with a xenoantiserum against the antigens showed that p3hr-1 cells have on their surface about 1% of the amount found on other human b lymphoblastoid cell lines. the deficit of the glycoproteins on the surface of p3hr-1 cells could be accounted for by a reduced rate of synthesis in these cells. a sim ... | 1977 | 72671 |
| radioimmunoassay for epstein--barr virus (ebv)-associated nuclear antigen (ebna). binding of iodinated antibodies to antigen immobilized in polyacrylamide gel. | 1977 | 73461 | |
| detection of a nuclear, ebna-type antigen in apparently ebna-negative herpesvirus papio (hvp)-transformed lymphoid lines by the acid-fixed nuclear binding technique. | in agreement with the findings of previous authors, we could not detect a virally determined nuclear antigen in herpesvirus papio (hvp)-transformed baboon lymphoid lines by anticomplementary staining in situ, as for ebna. however, by means of our recently developed acid-fixed nuclear binding technique an ebna-like antigen could be readily demonstrated, after extraction from both producer and non-producer lines. we propose to designate the antigen as hupna. it can be detected by a human anti-ebna ... | 1977 | 73523 |
| studies of an epstein-barr virus-induced dna polymerase. | 1977 | 14436 | |
| tumorigenicity of human hematopoietic cell lines in athymic nude mice. | human hematopoietic cell lines, which had been classified on the basis of studies on clonality, and morphological, chromosomal and functional parameters as lymphoblastoid cell lines (lcl) of presumed non-neoplastic origin, and lymphoma, myeloma and leukemia lines of proven malignant origin, were tested for tumorigenic potential on subcutaneous transplantation to nude mice and for capacity to grow in semi-solid medium in vitro. recently established lcl failed to grow both in nude mice and in agar ... | 1977 | 14896 |
| studies on epstein-barr virus-related antigens. ii. biochemical properties of soluble antigen in raji burkitt lymphoma cells. | biochemical properties of epstein-barr virus (ebv)-related soluble antigen in non-producer raji burkitt lymphoblastoid cells, assayed by the indirect single radial immunodiffusion (isrd) test, were investigated. the soluble isrd antigen retained activity even after exposure to a temperature of 80 degrees c. the antigen was precipitated in 40% saturated ammonium sulfate and isrd activity was recovered from the precipitate when reconstituted into a solution. isoelectric focusing and crossed immuno ... | 1977 | 14897 |
| appearance and some unusual features of epstein-barr virus antibody production in infectious mononucleosis and other health disorders. | the indirect immunofluorescence (if) test and the complement-fixation reaction (cfr) were used in examination of over 1500 sera obtained from patients with infectious mononucleosis (im) and other health disorders. the evidence obtained supports a direct aetiological relationship between epstein-barr virus (ebv) and im and points on a relationship of ebv to some other lymphadenopathies and health disorders. the incidence of the igg type antibody against virus capsid antigen (eb-vca) and soluble a ... | 1977 | 15437 |
| late persistence of serum gamma-glutamyl transpeptidase activity after mononucleosis. report of 3 cases. | gamma-glutamyl transpeptidase (ggtp) is a sensitive but nonspecific index hepatobiliary disease. in infectious mononucleosis (im) or the mononucleosis-like disease attributable to cytomegalovirus (cytomegalovirus-induced im), ggtp reverted to normal later than aspartate aminotransferase and alkaline phosphatase. in three cases elevated serum ggtp activity persisted for up to 24 months -- raising the question of persistent 'post-im' hepatitis. such prolonged ggtp activity was unusual in other lat ... | 1977 | 15921 |
| differential expression of d and r components of epstein-barr virus early antigen after superinfection and after induction with 5-iododeoxyuridine. | three non-productive human lymphoblastoid cell lines, nc-37, raji and l-1, either superinfected with epstein-barr (eb) virus from p3hr-1 cells or treated with 5-iododexyuridine (iudr), were studied for the development of early antigen (ea) of eb virus. in all three superinfected cell lines, both the restricted (r) and diffuse (d) componets of ea were synthesized, with r-component formation preceding d-component synthesis. after iudr treatment both components of ea were only formed in raji cells, ... | 1977 | 18915 |
| opportunistic non-hodgkin's lymphoma in x-linked recessive immunodeficiency and lymphoproliferative syndromes. | 1977 | 21454 | |
| detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity. | skin fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ... | 1977 | 64461 |
| is burkitt's lymphoma related to perinatal infection by epstein-barr virus? | burkitt's lymphoma (b.l.) is very restricted geographically, whereas the epstein-barr virus (e.b.v.), a very likely causal factor, exists all over the world. it is proposed that perinatal infection (either transplacental, or, more probably, neonatal) is an important risk factor for the development of b.l. this hypothesis arose when the epidemiological characteristics of e.b.v. infection were compared in different parts of the world and is supported by the preliminary results of a seroepidemiolog ... | 1977 | 64861 |
| complex pathogenesis of cancer in transplant recipients. | 1977 | 65239 | |
| comparative studies on ebv antigens by immunofluorescence and immunoperoxidase techniques. | three groups of ebv antigens, vca, ma and ea, were compared by the techniques of electron microscopic immunoperoxidase (ip) and immunofluorescence (if). p3hr-1 and ebv superinfected raji cells served as targets for labelled sera from patients with bl, npc and im or from healthy donors. 125i peroxidase-labelled antibodies were also prepared to determine, autoradiographically, the penetration of the complex into the cell system, and to monitor the incubation and washing procedures. the development ... | 1977 | 66208 |
| separation of the complement-fixing and early antigens from epstein-barr soluble antigen. | the epstein-barr virus-associated complement-fixing (soluble) antigen (cfsa) and the early antigen (ea) were isolated by ion-exchange chromatography from the p3hr-1 lymphoblastoid cell line activated with 5-iodo-2'-deoxyuridine for 72 hours. as expected, these two antigens were not immunologically identical. the cfsa, but not the ea, was isolated from the rajl lymphoblastoid cell line by the same method. | 1977 | 66320 |
| blastogenic response and ebna induction in human lymphocytes by epstein-barr virus only requires b cells but not macrophages. | the postulated role of macrophages in the primary infection of human lymphocytes by epstein-barr virus (ebv) was examined. macrophage removal had no effect on the blastogenic response but slightly reduced the percentage of ebna-positive cells induced by the b 95-8 virus strain. both the rate of dna synthesis and the appearance of ebna-positive cells could rather be related to the number of b cells in the infected populations. macrophages are thus not required for the initiation of ebv-induced ly ... | 1977 | 45722 |
| extrinsic modulation of human t-lymphocyte e rosette function associated with prolonged hepatocellular injury after viral hepatitis. | defective t-lymphocyte e rosette (er) function associated with viral hepatitis a and b may be due to mechanisms extrinsic or intrinsic to the target lymphocyte. the extrinsic defect is induced by an immunoregulatory plasma lipoprotein (rif) and has the capacity to regenerate er function in vitro. the intrinsic defect is refractory to regeneration and is not associated with rif. although both mechanisms occur with high frequency during the acute phase of viral hepatitis they tend to segregate in ... | 1977 | 187620 |
| establishment in continuous culture of a new type of lymphocyte from a "burkitt like" malignant lymphoma (line d.g.-75). | the isolation and establishment in vitro of a hitherto undescribed type of lymphocyte designated d.g.-75 is reported. the original inoculum was derived from the pleural effusion of a child with a primary abdominal lymphoma, which clinically and histologically resembled burkitt's lymphoma. in addition to the absence of the ebv genome and ebv receptors, this line possesses a number of other properties which distinguish it from previously described lymphoblastoid cell lines. it has different growth ... | 1977 | 188769 |
| a case control study on immunity to two epstein-barr virus-associated antigens, and to herpes simplex virus and adenovirus in a population-based group of patients with hodgkin's disease in denmark, 1971-73. | one hundred and eighty-five patients with untreated hodgkin's disease (hd) comprising 86% of all new cases diagnosed in denmark over a 2-year period, were individually matched with healthy controls of the same age, sex, and social class. in comparison to controls, hd patients showed significantly elevated mean antibody titres to epstein-barr viral capsid antierences in mean titres were found for adenovirus common antigen. subdivision of the patients by age, sex, social class, hl-a antigens, stag ... | 1977 | 188770 |
| elevated parainfluenza virus type 1 antibody in patients with subacute sclerosing panencephalitis. | parainfluenza virus hemagglutination inhibition (hi) antibodies were determined 3 times in the sera of 9 patients with subacute sclerosiing panencephalitis (sspe) and 20 healthy controls matched for age and place of residence. serum antibody against parainfluenza virus type 1 was significantly elevated in sspe patients as compared with controls, whereas antibodies against type 2 and 3 were found to be in normal ranges. higher titres of parainfluenza virus type 1 antibody might depend on: (1) dua ... | 1977 | 188993 |
| synthesis of epstein-barr virus dna in vitro: effects of phosphonoacetic acid, n-ethylmaleimide, and atp. | nuclei from superinfected raji cells synthesized epstein-barr virus (ebv) dna in vitro in the absence of cell dna synthesis. the synthesis of ebv dna in vitro was inhibited by phosphonoacetic acid and n-ethylmaleimide, and maximum synthesis was achieved in the absence of an atp-regenerating system. nuclei from mock-infected cells required an atp-regenerating system for maximum dna synthesis. | 1977 | 189079 |
| infectious mononucleosis in children. evaluation of epstein-barr virus-specific serological data. | epstein-barr virus (ebv)-specific antibody responses were determined in 43 consecutive pediatric patients who had signs and symptoms of inectious mononucleosis (im) and positive diagnostic tests for mononucleosis (monspot). thirty patients gave clear-cut serologic evidence of primary ebv infections; of the remaining 13 patients, seven had no antibodies to ebv in the acute- or convalescent-phase sera and six showed serologic patterns of past ebv infections. further testing proved that the initial ... | 1977 | 189099 |
| quantitative evaluation of epstein-barr-virus-infected mononuclear peripheral blood leukocytes in infectious mononucleosis. | we devised a quantitative assay for epstein-barr-virus-infected mononuclear leukocytes (virocytes) to determine their prevalence in the blood of patients with acute-phase and convalescent-phase infectious mononucleosis and in healthy epstein-barr-virus-seropositive controls. mononuclear peripheral blood leukocyte suspensions were tested for virus-determined cytoproliferative activity by cocultivation with human cord-cell indicator cultures. the highest levels of virocytes among circulating monon ... | 1977 | 187934 |
| patterns of oropharyngeal eb-virus shedding. | 1977 | 187936 | |
| infectious mononucleosis. | 1977 | 187809 | |
| detection of epstein-barr virus early antigen-d and its antibodies by passive hemagglutination. | these data describe the use of a passive hemagglutination test (phat) for the detection of antibodies to the epstein-barr virus (ebv) associated antigen, early antigen-d (ea-d). ea-d was obtained from ebv-superinfected rpmi 64-10 cell preparations which showed by immunofluorescent staining (if) that 25-35% of the cells contained ea-d. solubilization of ea-d was achieved by sonic oscillation, treatment of the pellet-associated material with triton x 100, ammonium sulfate precipitation, and chroma ... | 1977 | 190173 |
| suitability of epstein-barr virus dna obtained from superinfected raji cells for complementary rna hybridization studies. | a method was established to obtain a high yield of epstein-barr virus (ebv) dna for nucleic acid hybridization studies on latent virus dna in transformed cells. superinfection of raji cells with ebv concentrated from hri cell cultures produced a 600-fold higher yield of ebv dna than direct isolation of ebv from hri cell cultures. the virus dna thus prepared from raji cells superinfected with ebv was radioisotopically and spectrophotometrically pure and served as a satisfactory template for the p ... | 1977 | 190349 |
| antibodies to the r component of epstein-barr virus-induced early antigens in burkitt's lymphoma exceeding in titer antibodies to epstein-barr capsid antigen. | african patients with burkitt's lymphoma (bl) may show antibodies to the r (restricted) component of the epstein-barr (eb) virus-induced early antigens that appear to match in titer the antibodies to eb viral capsid antigen (vca) as determined with the aid of acetone-fixed smears of eb3 cells. virus-producing eb3 cells, however, contain the r component as well as vca so that the correct anti-vca titers in such cases remain in doubt. since the r component, but not vca, was found to be denatured b ... | 1977 | 190417 |