Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| correlating differences in larval survival and development of bollworm (lepidoptera: noctuidae) and fall armyworm (lepidoptera: noctuidae) to differential expression of cry1a(c) delta-endotoxin in various plant parts among commercial cultivars of transgenic bacillus thuringiensis cotton. | differences in larval survival and development of bollworm, helicoverpa zea (boddie), and fall armyworm, spodoptera frugiperda (j. e. smith), respectively, were found to exist among commercially available cry1a(c) transgenic bacillus thuringiensis berliner (bt) varieties. using a quantification assay (elisa) to measure the levels of delta-endotoxin in two of these varieties ('dp 451b/rr' and 'nucotn 33b'), differences in the amount of delta-endotoxin present in various plant parts was correlated ... | 2001 | 11233127 |
| identification of a novel isoform of the cyclic-nucleotide phosphodiesterase pde3a expressed in vascular smooth-muscle myocytes. | we have identified a new cyclic-nucleotide phosphodiesterase isoform, pde3a, and cloned its cdna from cultured aortic myocytes. the nucleotide sequence of its coding region is similar to that of the previously cloned myocardial isoform except for the absence of the initial 300-400 nt that are present in the latter, as confirmed by reverse-transcriptase-mediated pcr, 5' rapid amplification of cdna ends and a ribonuclease protection assay. expression in spodoptera frugiperda (sf9) cells yields a p ... | 2001 | 11115397 |
| interaction of hydrophobic anions with the rat skeletal muscle chloride channel clc-1: effects on permeation and gating. | permeation of a range of hydrophobic anions through the rat skeletal muscle chloride channel, rclc-1, expressed in sf-9 (a spodoptera frugiperda insect cell line) cells has been studied using the whole-cell patch-clamp technique. bi-ionic reversal potentials measured with external application of foreign anions gave the following permeability sequence: cl- (1) > benzoate (0.15) > hexanoate (0.12) > butyrate (0.09) > propionate (0.047) approximately formate (0.046). anions with larger hydrophobic ... | 2001 | 11158270 |
| production of functional antibodies generated in a nonlytic insect cell expression system. | a monoclonal antibody directed against the type 2 adenovirus (ad2) penton base protein was cloned and expressed in spodoptera frugiperda (sf9) cells using a nonlytic vector system. the coding sequences for the immunoglobulin light and heavy chains were placed under the control of the orgyia pseudotsugata multicapsid nucleopolyhedrosis virus immediate-early 2 (opie2) promoter. transfected sf9 cells continuously secreted the antibody which retained the ability to recognize both native and recombin ... | 2001 | 11162396 |
| high-level expression of human acidic fibroblast growth factor and basic fibroblast growth factor in silkworm (bombyx mori l.) using recombinant baculovirus. | a hybrid of autographa californica nuclear polyhedrosis virus and bombyx mori nuclear polyhedrosis virus, which is infectious to both spodoptera frugiperda and bombyx mori, was prepared in our previous study. two recombinant hybrid baculoviruses, carrying cdnas of human acidic and basic fibroblast growth factors, respectively, were successfully constructed in this study, for the large-scale production of human afgf and bfgf using silkworm as host. these recombinant viruses were used to inoculate ... | 2001 | 11162406 |
| alpha-galactosidases from the larval midgut of tenebrio molitor (coleoptera) and spodoptera frugiperda (lepidoptera). | there are three midgut alpha-galactosidases (tg1, tg2, tg3) from tenebrio molitor larvae that are partially resolved by ion-exchange chromatography. the enzymes have approximately the same ph optimum (5.0), pl value (4.6) and mr value (46000-49000) as determined by gel filtration or native electrophoresis run in polyacrylamide gels with different concentrations. substrate specificities and functions were proposed for the major t. molitor midgut alpha-galactosidases (tg2 and tg3) based on chromat ... | 2001 | 11163310 |
| 5-hydroxytryptamine 4(a) receptor expressed in sf9 cells is palmitoylated in an agonist-dependent manner. | the mouse 5-hydroxytryptamine 4(a) receptor [5-ht(4(a))] was expressed with a baculovirus system in insect cells and analysed for acylation. [(3)h]palmitic acid was effectively incorporated into 5-ht(4(a)) and label was sensitive to the treatment with reducing agents indicating a thioester-type bond. analysis of protein-bound fatty acids revealed that 5-ht(4(a)) contains predominantly palmitic acid. treatment of infected sf9 (spodoptera frugiperda) cells with bimu8 [(endo-n-8-methyl-8-azabicyclo ... | 2001 | 11171060 |
| expression of scavenger receptor class b type 1 (sr-bi) promotes microvillar channel formation and selective cholesteryl ester transport in a heterologous reconstituted system. | in the "selective" cholesteryl ester (ce) uptake process, surface-associated lipoproteins [high density lipoprotein (hdl) and low density lipoprotein] are trapped in the space formed between closely apposed surface microvilli (microvillar channels) in hormone-stimulated steroidogenic cells. this is the same location where an hdl receptor (sr-bi) is found. in the current study, we sought to understand the relationship between sr-bi and selective ce uptake in a heterologous insect cell system. sf9 ... | 2001 | 11171999 |
| characterization of the cdna encoding the 90 kda heat-shock protein in the lepidoptera bombyx mori and spodoptera frugiperda. | this report presents the first hsp90 complete cdna sequences from two lepidoptera. the bombyx mori full sequence was reconstituted from 15 partial cdna clones belonging to expressed sequence tag libraries obtained from different tissues or cultured cells, thus showing the ubiquitous expression of the hsp90 gene. the spodoptera frugiperda cdna was isolated as a full-length clone from a cdna library established from the sf9 cell line. both cdnas are highly homologous and display the classical amin ... | 2001 | 11418243 |
| acetylcholinesterase and insect growth inhibitory activities of gutierrezia microcephala on fall armyworm spodoptera frugiperda j.e. smith. | from the aerial parts of gutierrezia microcephala (asteraceae), four oxyflavones were isolated, namely 5,7,2'-trihydroxy-3,6,8,4',5'-pentamethoxyflavone (1); 5,7,4'-trihydroxy-3,6,8-trimethoxyflavone (2); 5,7,2',4'-tetrahydroxy-3,6,8,5'-tetramethoxyflavone (3); 5,2'-dihydroxy-3,6,7,8,4',5'-hexamethoxyflavone (4), and an ent-clerodane, bacchabolivic acid (5). compounds 1-5, the synthetic methyl ester (6), n-hexane and meoh extracts were evaluated against the fall armyworm (spodoptera frugiperda). ... | 2001 | 11421454 |
| impact of bt cottons expressing one or two insecticidal proteins of bacillus thuringiensis berliner on growth and survival of noctuid (lepidoptera) larvae. | a series of laboratory assays were performed to compare the relative impact of commercial and experimental cultivars of cotton, gossypium hirsutum (l.), expressing zero, one, or two insecticidal proteins of bacillus thuringiensis berliner, on several lepidopteran pests. assays in which larvae were fed fresh plant tissue indicated that dual-toxin b. thuringiensis (bt) cultivars, expressing both cry1ac and cry2ab endotoxins of b. thuringiensis, were more toxic to bollworms, helicoverpa zea (boddie ... | 2001 | 11425033 |
| tyr199 in transmembrane domain 5 of the beta2-adrenergic receptor interacts directly with the pharmacophore of a unique fluorenone-based antagonist. | mutagenesis of the beta2-adrenergic receptor (beta2ar) has suggested that amino acids in transmembrane domain 5 (tmd 5) play an important role in the interaction of the receptor with the catechol end of adrenergic agonists. however, little direct biochemical evidence for the interaction of any beta2ar agonist or antagonist with tmd 5 has been reported. to identify receptor amino acids that contribute to the beta2ar antagonist binding site, we identified the precise amino acid photoinsertion site ... | 2001 | 11237852 |
| effect of baculovirus infection on the mrna and protein levels of the spodoptera frugiperda eukaryotic initiation factor 4e. | the cdna sequence of eukaryotic translation initiation factor eif4e was derived from a spodoptera frugiperda cdna library. eight tryptophan residues, typical for eif4e, are strictly conserved in the encoded 210 amino acid protein. a polyclonal antiserum detected a 26 kda protein in lepidopteran cell lines, but not in dipteran cells. sf21 cells have a single eif4e gene copy, which is transcribed into a 1500 nt transcript. infection with acmnpv resulted in a decrease in eif4e mrna starting between ... | 2001 | 11437917 |
| production of biologically active recombinant bovine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae. | the full-length bovine interferon-gamma (bifn-gamma) cdna, including the secretion signal peptide coding region was recloned into baculovirus transfer vectors pacym1 and pbm050. these vectors were co-transfected with autographa californica nuclear polyhedrosis virus (acnpv) or bombyx mori nuclear polyhedrosis virus (bmnpv) dna into spodoptera frugiperda cells (sf21ae) and bombyx mori cells (bmn), respectively. the recombinant viruses, named acbifn-gamma and bmbifn-gamma, were then recovered. rec ... | 2001 | 11145838 |
| expression of the human mu opioid receptor in a stable sf9 cell line. | the cdna that encodes the human mu opioid receptor (hmor) has been cloned and expressed in spodoptera frugiperda (sf9) cells using a nonlytic vector system. the coding sequence fused to the cleavable glycoprotein signal peptide gp 64, and a c-terminal histidine tag was placed under the transcriptional control of the orgyia pseudotsugata multicapsid nucleopolyhedrosis virus immediate-early 2 (opie2) promoter. transfected cells were selected using zeocin resistance and the receptor was constitutiv ... | 2002 | 11911927 |
| functional characterization of the type 1 inositol 1,4,5-trisphosphate receptor coupling domain sii(+/-) splice variants and the opisthotonos mutant form. | the type 1 inositol (1,4,5)-trisphosphate receptor (insp3r1) plays a critical role in ca2+ signaling in cells. neuronal and nonneuronal isoforms of the insp3r1 differ by alternative splicing in the coupling domain of the insp3r1 (sii site). deletion of 107 amino acids from the coupling domain of the insp3r1 results in epileptic-like behaviors in opisthotonos (opt) spontaneous mouse mutant. using spodoptera frugiperda cells expression system, we compared single-channel behavior of recombinant ins ... | 2002 | 11916857 |
| 5-hydroxytryptamine 4(a) receptor is coupled to the galpha subunit of heterotrimeric g13 protein. | serotonin (5-hydroxytryptamine (5-ht)) is an important neurotransmitter that regulates multiple events in the central nervous system. many of the 5-ht functions are mediated via g protein-coupled receptors that are coupled to multiple heterotrimeric g proteins, including g(s), g(i), and g(q) subfamilies (martin, g. r., eglen, r. m., hamblin, m. w., hoyer, d., and yocca, f. (1998) trends pharmacol. sci. 19, 2-4). here we show for the first time that the 5-hydroxytryptamine 4(a) receptor (5-ht(4(a ... | 2002 | 11923294 |
| fluorometric microplate assay to measure glutathione s-transferase activity in insects and mites using monochlorobimane. | elevated levels of glutathione s-transferases (gsts) play a major role as a mechanism of resistance to insecticides and acaricides in resistant pest insects and mites, respectively. such compounds are either detoxicated directly via phase i metabolism or detoxicated by phase ii metabolism of metabolites as formed by microsomal monooxygenases. here we used monochlorobimane (mcb) as an artificial substrate and glutathione to determine total gst activity in equivalents of single pest insects and sp ... | 2002 | 11950219 |
| biochemical properties of purified recombinant human beta-carotene 15,15'-monooxygenase. | beta-carotene 15,15'-monooxygenase (bco), formerly known as beta-carotene 15,15'-dioxygenase, catalyzes the first step in the synthesis of vitamin a from dietary carotenoids. we have biochemically and enzymologically characterized the purified recombinant human bco enzyme. a highly active bco enzyme was expressed and purified to homogeneity from baculovirus-infected spodoptera frugiperda 9 insect cells. the k(m) and v(max) of the enzyme for beta-carotene were 7 microm and 10 nmol retinal/mg x mi ... | 2002 | 11960992 |
| the high-affinity calcium[bond]calmodulin-binding site does not play a role in the modulation of type 1 inositol 1,4,5-trisphosphate receptor function by calcium and calmodulin. | modulation of the inositol 1,4,5-trisphosphate (insp(3)) receptors (insp(3)r) by cytosolic calcium (ca(2+)) plays an essential role in ca(2+) signalling, but structural determinants and mechanisms responsible for the insp(3)r regulation by ca(2+) are poorly understood. in the present study, we expressed rat insp(3)r type 1 (insp(3)r1) in spodoptera frugiperda cells using a baculovirus-expression system and reconstituted the recombinant insp(3)r1 into planar lipid bilayers for functional analysis ... | 2002 | 11972451 |
| purification and biochemical characterization of some of the properties of recombinant human kynureninase. | recombinant human kynureninase (l-kynurenine hydrolase, ec 3.7.1.3) was purified to homogeneity (60-fold) from spodoptera frugiperda (sf9) cells infected with baculovirus containing the kynureninase gene. the purification protocol comprised ammonium sulfate precipitation and several chromatographic steps, including deae-sepharose cl-6b, hydroxyapatite, strong anionic and cationic separations. the purity of the enzyme was determined by sds/page, and the molecular mass verified by maldi-tof ms. th ... | 2002 | 11985583 |
| biological activity of astilbin from dimorphandra mollis against anticarsia gemmatalis and spodoptera frugiperda. | astilbin was isolated in high yield from dimorphandra mollis, and its insecticidal and growth inhibiting activity by stomach ingestion were evaluated against anticarsia gemmatalis and spodoptera frugiperda. the insecticidal activity of astilbin, the weight reduction of the larval phase and the prolongation of the larval and pupal phases were verified for both species. astilbin was identified on the base of its nmr, ms and physical data. | 2002 | 11997979 |
| grass species and endophyte effects on survival and development of fall armyworm (lepidoptera: noctuidae). | grass selections including 10 zoysiagrasses, 18 paspalums, 34 bermuda grasses, tall fescue, creeping red fescue, and perennial ryegrasses with and without endophyte were evaluated for potential resistance to fall armyworm, spodoptera frugiperda (j. e. smith), larvae. laboratory evaluations assessed the degree of antibiosis among >70 grass lines to first-instar fall armvworms. when all parameters measured were considered, the trend in resistance to fall armyworm among endophyte-infected (e+) and ... | 2002 | 12020031 |
| molecular cloning and induction of nuclear receptors from insect cell lines. | fragments of ecr and usp were cloned from two insect cell lines, sf21 and high five cells (derived respectively from spodoptera frugiperda and trichoplusia ni), using a pcr-based approach employing degenerate primers designed on the basis of conserved regions of nuclear receptors, together with 5'- and 3'-race. an additional orphan nuclear receptor, hr4 fragment, was cloned from high five cells. comparison of these fragments with manduca sexta counterparts showed that the cloned sfecr [ecdysone ... | 2002 | 12020840 |
| identification of protein tyrosine phosphatase 1b and casein as substrates for 124-v-mos. | the mos proto-oncogene encodes a cytoplasmic serine/threonine-specific protein kinase with crucial function during meiotic cell division in vertebrates. based on oncogenic amino acid substitutions the viral derivative, 124-v-mos, displays constitutive protein kinase activity and functions independent of unknown upstream effectors of mos protein kinase. we have utilized this property of 124-v-mos and screened for novel mos substrates in immunocomplex kinase assays in vitro. | 2002 | 12022922 |
| expression of the movement protein of tomato spotted wilt virus in its insect vector frankliniella occidentalis. | tomato spotted wilt virus (tswv) is able to infect both its botanical hosts and its insect vector (thrips). in plant tissue the ns(m) protein of tswv functions as viral movement protein (mp), aggregating into plasmodesma-penetrating tubules to establish cell-to-cell movement. as upon heterologous expression ns(m) was able to form similar tubules on the surface of insect (spodoptera frugiperda) cells, we have now investigated the expression and cellular manifestation of this protein in infected t ... | 2002 | 12038691 |
| chromosome instability in spodoptera frugiperda sf-9 cell line. | homogeneous cell lines are essential in industry and research if reliable and reproducible data are to be obtained. the spodoptera frugiperda (sf-9) cell line routinely used for the production of recombinant proteins was found to be heterogeneous, containing a mixture of diploid and tetraploid cells. using dilution-cloning techniques, diploid and tetraploid subpopulations were isolated from a sf-9 parental cell line, and their cytogenetic state was monitored using vinblastine to arrest cells in ... | 2002 | 12052082 |
| enzyme kinetics and glycan structural characterization of secreted alkaline phosphatase prepared using the baculovirus expression vector system. | secreted human alkaline phosphatase (seap, a model protein containing a single n-glycan chain) was expressed in spodoptera frugiperda sf-9 (sf-9) and trichoplusia ni bti-tn-5b1-4 (tn-5b1-4) insect cell lines infected with recombinant autographa californica multiple nuclear polyhedrovirus expressing seap under control of the polyhedrin promoter. sds-page showed that both systems expressed fairly pure rseap products. the rseap expression level was 7.0 u/ml in tn-5b1-4, higher than the 4.1 u/ml pro ... | 2002 | 12109816 |
| optimization of baculovirus-mediated expression and purification of hexahistidine-tagged murine dna (cytosine-c5)-methyltransferase-1 in spodoptera frugiperda 9 cells. | enzymatic dna methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. dna (cytosine-c5)-methyltransferase-1 is the enzyme responsible for maintaining established methylation patterns during replication in mammalian cells. it is composed of a large ( approximately 1100 amino acids (a.a.)) amino-terminal region containing many putative regulatory domains and a smaller ( approximately 500 a.a.) carboxy-t ... | 2002 | 12071696 |
| lack of involvement of haemocytes in the establishment and spread of infection in spodoptera frugiperda larvae infected with the baculovirus autographa californica m nucleopolyhedrovirus by intrahaemocoelic injection. | it is thought that insect haemocytes, or blood cells, play an important role in baculovirus pathogenesis by amplifying and helping to spread the infection within the insect. here, infection is described of the lepidopteran noctuid species spodoptera frugiperda with the baculovirus autographa californica m nucleopolyhedrovirus (acmnpv). late instar s. frugiperda larvae were infected by intrahaemocoelic injection using a recombinant of acmnpv expressing the enhanced green fluorescent protein gene ... | 2002 | 12075074 |
| the human histamine h2-receptor couples more efficiently to sf9 insect cell gs-proteins than to insect cell gq-proteins: limitations of sf9 cells for the analysis of receptor/gq-protein coupling. | the human histamine h2-receptor (hh2r) couples to gs-proteins to activate adenylyl cyclase and to gq-proteins to activate phospholipase c, but phospholipase c activation has not consistently been observed. the aim of this study was to compare coupling of hh2r to insect and mammalian gs- and gq-proteins in spodoptera frugiperda (sf9) cells. interaction of hh2r with mammalian g proteins was assessed with coexpressed proteins or receptor-galpha fusion proteins that enhance coupling efficiency. hh2r ... | 2002 | 11841575 |
| insect antifeedant activity of tetranortriterpenoids from the rutales. a perusal of structural relations. | structure-related insect antifeedant relationship of 56 limonoids (both natural and modified) from the plants belonging to the order rutales was attempted considering substitution patterns, oxidation states, and hydrophobicity, as well as distant geometry derived through conformational analysis on molecular modeling. orientation of the furan and hydroxylation at specific carbon sites have been shown to influence the antifeedancy against the fall armyworm, spodoptera litura. | 2002 | 12137465 |
| combined analysis of supernatant-based feeding bioassays and pcr as a first-tier screening strategy for vip -derived activities in bacillus thuringiensis strains effective against tropical fall armyworm. | to assess whether feeding bioassays using culture-supernatant proteins could be combined with pcr into a first-tier screening strategy for vip3a-like genes efficient against tropical spodoptera frugiperda. | 2002 | 12147075 |
| heterologous expression and topography of the main intrinsic protein (mip) from rat lens. | wild type rat lens main intrinsic protein (mip) and mip mutated (f73i, f75l) to resemble the glycerol facilitator of escherichia coli in the region of the npa1 box were used to investigate the topology of mip in the membrane of spodoptera frugiperda (sf21) cells using the baculovirus expression system and expression in mouse erythroid leukaemia cells (mel c88). differential fixation for staining was used, with paraformaldehyde for externally exposed antigenic sites, and acetone for both external ... | 2002 | 11852078 |
| ph-dependent channel activity of heterologously-expressed main intrinsic protein (mip) from rat lens. | wild-type rat lens main intrinsic protein (mip) was heterologously expressed in the membrane of spodoptera frugiperda (sf21) cells using the baculovirus expression system and in mouse erythroid leukaemia cells (mel c88). both mel and sf21 cell lines expressing wild-type mip were investigated for the conductance of ions using a whole cell patch clamp technique. an increase in conductance was seen in both expression systems, particularly on lowering the ph to 6.3. in sf21 cells, addition of antibo ... | 2002 | 11852079 |
| kinetic properties of bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from spinach leaves. | a cdna encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a spinacia oleracea leaf library and used to express a recombinant enzyme in escherichia coli and spodoptera frugiperda cells. the insoluble protein expressed in e. coli was purified and used to raise antibodies. western blot analysis of a protein extract from spinach leaf showed a single band of 90.8 kda. soluble protein was purified to homogeneity from s. frugiperda cells infected with recombinant baculoviru ... | 2002 | 11856361 |
| diversity of bacillus thuringiensis strains from colombia with insecticidal activity against spodoptera frugiperda (lepidoptera:noctuidae). | to identify and characterize bacillus thuringiensis strains highly toxic to spodoptera frugiperda, and to explore the genetic diversity of such strains. | 2002 | 11872122 |
| dual signalling by different octopamine receptors converges on adenylate cyclase in sf9 cells. | the sf9 insect cell line, derived from spodoptera frugiperda, was used to study the regulation of adenylate cyclase (ac) activity by octopamine receptors. the cyclic amp (camp) production was stimulated in a concentration-dependent manner by octopamine. octopamine also elicited a rise in cytosolic ca(2+). the ca(2+) elevation was independent of the camp elevation whereas the camp elevation was partially inhibited by removal of ca(2+). the antagonistic effects of a series of compounds were tested ... | 2002 | 11804800 |
| assessment of foreign protein production by recombinant heliothis (helicoverpa) armigera entomopoxviruses in spodoptera frugiperda cells. | this report describes the first production of recombinant forms of heliothis (helicoverpa) armigera entomopoxvirus (haepv). these haepvs are engineered at either the spheroidin or fusolin locus, to produce the green fluorescent marker protein (gfp). the growth properties of these recombinant haepvs, in comparison to the parental haepv, were assessed in cultured spodoptera frugiperda sf9 cells. additionally, gfp production by these recombinant haepvs was compared to that of a gfp-expressing recom ... | 2002 | 11807239 |
| 5-ht-receptor-induced changes of the intracellular camp level monitored by a hyperpolarization-activated cation channel. | the hvcng channel from the moth heliothis virescens is highly sensitive to camp concentrations ranging between 0.1 microm and 5 microm. this hvcng channel was over-expressed in spodoptera frugiperda (sf.9) cells to measure endogenous camp levels. hyperpolarization-activated inward currents were measured in the whole-cell patch-clamp configuration with pipettes filled with different camp concentrations to calibrate the system. varying the camp concentration between 0 microm and 100 microm in the ... | 2002 | 11810212 |
| distribution and characterization of bacillus thuringiensis on the phylloplane of species of piper (piperaceae) in three altitudinal levels. | bacillus thuringiensis is found naturally on the phylloplane. in this study 35 samples from 13 species of the genus piper (piperaceae) were collected from three altitudinal levels located between 1800 and 2900 m above sea level in the colombian andean forest of central cordillera. two hundred and fifty-six isolates of b. thuringiensis were obtained from 74% of the samples studied. b. thuringiensis index (number of isolates of b. thuringiensis/number of isolates of sporulated bacilli) was 0.2. th ... | 2002 | 12087427 |
| characterization of wolbachia host cell range via the in vitro establishment of infections. | maternally transmitted bacteria of the genus wolbachia are obligate, intracellular symbionts that are frequently found in insects and cause a diverse array of reproductive manipulations, including cytoplasmic incompatibility, male killing, parthenogenesis, and feminization. despite the existence of a broad range of scientific interest, many aspects of wolbachia research have been limited to laboratories with insect-rearing facilities. the inability to culture these bacteria outside of the invert ... | 2002 | 11823204 |
| endonucleases that may recognize the ssdna-backbone structure: purification from sf9 cells and characterization. | endonucleases that cleave ssdna under conditions that minimize the formation of secondary structures were detected in spodoptera frugiperda sf9 cells. one endonuclease was purified from sf9 cells and another from sf9 cells infected by baculoviruses. polynucleotides containing an a- or t-tract, or a cap binding region were digested in the presence of atp at low mg ions with these two nucleases. atp could be replaced by citrate but not by edta. the cleavage patterns were different from those obtai ... | 2002 | 12489994 |
| toxicities of emamectin benzoate homologues and photodegradates to lepidoptera. | the toxicity of a number of emamectin benzoate homologues and photodegradates to five species of lepidoptera was investigated using diet and foliar bioassays. the emamectin benzoate homologues b1a and b1b were equally toxic in the diet and foliar assays to spodoptera exigua (hübner), heliothis virescens (f.), tricoplusia ni (hübner), and spodoptera frugiperda (j. e. smith), within each of these species. plutella xylostella (l.) was the most sensitive species to emamectin benzoate. the ab1a photo ... | 2002 | 12539830 |
| beta-(1 --> 4)-galactosyltransferase activity in native and engineered insect cells measured with time-resolved europium fluorescence. | to evaluate the ability of insect cells to produce complex-type n-glycans, beta-(1 --> 4)-galactosyltransferase (beta4galt) activity in several insect cell lines was analyzed. for this purpose, we developed a simple and highly sensitive assay for beta-(1 --> 4)-galactosyltransferase (beta4galt) activity, which is based on time-resolved fluorometry of europium. bovine serum albumin (bsa) modified with glcnac (glcnac(44)-bsa) was used as the acceptor. glcnac(44)-bsa was coated on a 96-well micropl ... | 2002 | 12433481 |
| functional properties of a rainbow trout cyp3a27 expressed by recombinant baculovirus in insect cells. | cytochrome p450 3a27 (cyp3a27) is highly expressed in liver and intestine of rainbow trout (oncorhynchus mykiss). in many animal species, the intestine and liver are responsible for the first-pass metabolism of a wide range of xenobiotics. to help determine its physiological role, the catalytic capabilities of cyp3a27 protein were examined. an open reading frame of cyp3a27 in pfastbac donor plasmid was transferred to the baculovirus genome (bacmid dna) through tn7 site-specific transposition in ... | 2002 | 12433811 |
| expression of biologically active recombinant equine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae. | the full-length equine interferon-gamma (eifn-gamma) cdna, including the secretion signal peptide coding region, was recloned into baculovirus transfer vector pacym1. this vector was co-transfected with autographa californica nuclear polyhedrosis virus dna or hybrid nuclear polyhedrosis virus dna into spodoptera frugiperda cells. the recombinant viruses, named aceifn-gamma and hyeifn-gamma, were then recovered. recombinant eifn-gamma (reifn-gamma) was accumulated in the culture fluid of the acei ... | 2002 | 12445800 |
| use of the piggybac transposon for germ-line transformation of insects. | germ-line transformation of insects is now possible with four independent transposable element vector systems. among these, the ttaa-insertion site specific transposon, piggybac, discovered in trichoplusia ni, is one of the most widely used. transformations have been achieved in a wide variety of dipterans, lepidopterans, and a coleopteran, and for many species, piggybac transposition was first tested by plasmid-based mobility assays in cell lines and embryos. all plasmid and genomic insertions ... | 2002 | 12225912 |
| functional expression of a cdna encoding pea (pisum sativum l.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis. | raffinose (o-alpha- d-galactopyranosyl-(1-->6)- o-alpha- d-glucopyranosyl-(1<-->2)- o-beta- d-fructofuranoside) is a widespread oligosaccharide in plant seeds and other tissues. raffinose synthase (ec 2.4.1.82) is the key enzyme that channels sucrose into the raffinose oligosaccharide pathway. we here report on the isolation of a cdna encoding for raffinose synthase from maturing pea ( pisum sativum l.) seeds. the coding region of the cdna was expressed in spodoptera frugiperda sf21 insect cells ... | 2002 | 12244450 |
| parasitoid-mediated transmission of an iridescent virus. | we examined the interaction between an invertebrate iridescent virus (iiv) isolated from spodoptera frugiperda (j.e. smith) and the solitary ichneumonid endoparasitoid eiphosoma vitticolle cresson. in choice tests, parasitoids examined and stung significantly more virus infected than healthy larvae, apparently due to a lack of defense reaction in virus infected hosts. parasitoid-mediated virus transmission was observed in 100% of the female parasitoids that stung a virus infected host in the lab ... | 2002 | 12384082 |
| p53 autoantibodies in sera from danish ovarian cancer patients and their correlation with clinical data and prognosis. | the p53 gene, a tumour suppressor gene located on the short arm of chromosome 17 (17p13), is frequently mutated in various human tumours. accumulation of p53 protein in neoplastic cells and its release following tumour necrosis can lead to development of circulating autoantibodies (aab) against p53. earlier studies of ovarian cancer (oc) patients reported different frequencies of p53 aab and conclusions regarding the clinical and prognostic value of these aab have not been in agreement. we there ... | 2002 | 12390412 |
| the drosophila diap1 protein is required to prevent accumulation of a continuously generated, processed form of the apical caspase dronc. | although loss of the inhibitor of apoptosis (iap) protein diap1 has been shown to result in caspase activation and spontaneous cell death in drosophila cells and embryos, the point at which diap1 normally functions to inhibit caspase activation is unknown. depletion of the diap1 protein in drosophila s2 cells or the sf-iap protein in spodoptera frugiperda sf21 cells by rna interference (rnai) or cycloheximide treatment resulted in rapid and widespread caspase-dependent apoptosis. co-silencing of ... | 2002 | 12397080 |
| citrus limonoids and their semisynthetic derivatives as antifeedant agents against spodoptera frugiperda larvae. a structure-activity relationship study. | the antifeedant activity of citrus-derived limonoids limonin (1), nomilin (2), and obacunone (3) and their semisynthetic derivatives 4-26 was evaluated against a commercially important pest, spodoptera frugiperda. simple chemical conversions were carried out on the natural limonoids obtained from seeds of citrus limon. these conversions focused on functional groups considered to be important for the biological activity, namely the c-7 carbonyl and the furan ring. in particular, reduction at c-7 ... | 2002 | 12405773 |
| baculovirus lef-12 is not required for viral replication. | the baculovirus lef-12 (orf41) gene is required for transient expression of baculovirus late genes. to analyze the role of lef-12 in the context of infected cells, two mutant viruses were constructed. both mutants were viable in trichoplusia ni high 5 and spodoptera frugiperda sf9 cells. single-step growth curves, however, indicated that virus yields were reduced approximately fivefold in the absence of lef-12. pulse-labeling of infected cells revealed that lef-12 mutant viruses entered the late ... | 2002 | 12414945 |
| production of hybrid double- or triple-layered virus-like particles of group a and c rotaviruses using a baculovirus expression system. | dual infections by group a and group c rotaviruses have been reported, but no reassortants between group a and group c rotaviruses have been described. the vp6 major inner capsid protein of group a and c rotaviruses shares common antigens detected by monoclonal antibodies and also shares 40-43% amino acid identity. coinfection of spodoptera frugiperda (sf9) insect cells with different combinations of the recombinant baculoviruses encoding either group a [rf vp2 (a-vp2), ind vp6 (a-vp6), and vp7 ... | 2002 | 12429511 |
| biosynthesis and processing of spodoptera frugiperda alpha-mannosidase iii. | we previously cloned a lepidopteran insect cell cdna that encodes a class ii alpha-mannosidase that is localized in the golgi apparatus but is cobalt-dependent, has a neutral ph optimum, hydrolyzes man(5)glcnac(2) to man(3)glcnac(2), and cannot hydrolyze glcnacman(5)glcnac(2). this enzyme was designated sfmaniii to distinguish it from golgi alpha-mannosidase ii and indicate its derivation from the fall armyworm spodoptera frugiperda. in the present study, we prepared a polyclonal antibody and us ... | 2002 | 12107079 |
| the role of amino-acid residues q39 and e451 in the determination of substrate specificity of the spodoptera frugiperda beta-glycosidase. | the design of beta-glycosidases with planed substrate specificity for biotechnological application has received little attention. this is mostly a consequence of the lack of data on the molecular basis of the beta-glycosidase specificity, namely data on the energy of the noncovalent interactions in the enzyme-transition state complex. in an attempt to fill this gap, site-directed mutagenesis and enzyme steady-state kinetic experiments with different substrates were conducted, using as model a di ... | 2002 | 12153567 |
| functional analysis of a conserved region of the baculovirus envelope fusion protein, ld130. | the envelope fusion protein from a baculovirus pathogenic for lymantria dispar was characterized. n-terminal sequence analysis determined that it was cleaved downstream of predicted signal peptide and furin cleavage motifs. mutation of the furin motif resulted in a protein that was not cleaved and did not mediate fusion. mutagenesis of three charged amino acids in a conserved sequence with the features of a fusion peptide resulted in significant reduction of the ability of the constructs to medi ... | 2002 | 12490405 |
| juvenile hormone biosynthesis in the fall armyworm, spodoptera frugiperda (lepidoptera, noctuidae). | the in vitro production of juvenile hormones (jh) was investigated by using corpora allata (ca) of larvae and corpora cardiaca-corpora allata (cc-ca) complexes of adult females of the fall armyworm spodoptera frugiperda. in female moths, jh release was high compared to that in 5th and 6th instar larvae. concentrations of 0.11-0.12 mm methionine, 180-200 mm na(+), 5.8-8.3 mm k(+), 10-50 mm ca(2+) and a ph range of 5.7-6.3 yielded optimal incorporation of l-[methyl-(3)h] methionine in vitro by cc- ... | 2002 | 11997221 |
| expression, purification, and characterization of human dipeptidyl peptidase iv/cd26 in sf9 insect cells. | the human dipeptidyl peptidase iv/cd26 (dppiv/cd26) is a multifunctional type-ii membrane bound glycoprotein. as a receptor of collagen i and fibronectin it mediates cell-cell and cell-matrix adhesion, and by interacting with extracellular adenosine deaminase and cd45 it is involved in regulatory and costimulatory events in the immune system. dppiv/cd26 has a very distinct substrate specificity, and is potentially capable of truncating many cytokines, chemokines, and peptide hormones. in this st ... | 2002 | 12182835 |
| a approximately 35 kda polypeptide from insect cells binds to yeast acs like elements in the presence of atp. | the s. cerevisiae origin recognition complex binds to the ars consensus sequence in an atp dependent fashion. recently, the yeast cdc6 has been reported to have dna binding activity. conservation of replication proteins among different species strongly supports their functional similarity. here we report the results of an investigation into the dna binding activity of human cdc6 protein. cdc6 was expressed and purified from baculovirus infected sf9 (spodoptera frugiperda) insect cells as gst fus ... | 2002 | 12186657 |
| insect antifeedants from tropical plants ii: structure of zumsin. | a novel a-seco limonoid was isolated from methanolic extract of croton jatrophoides and designated as zumsin. this compound showed potent antifeedant activity against two lepidopteran larvae, pink bollworm, pectinophora gossypiella (pc(50) = 1 microg/cm(2), pc(95) = 8 microg/cm(2)), and fall armyworm, spodoptera frugiperda (pc(50) = 2 microg/cm(2), pc(95) = 16 microg/cm(2)). the structure of zumsin was determined as 1 using a variety of spectroscopic methods including nuclear magnetic resonance, ... | 2002 | 12188606 |
| thiersinines a and b: novel antiinsectan indole diterpenoids from a new fungicolous penicillium species (nrrl 28147). | [structure: see text] two novel antiinsectan indole diterpenoids, thiersinines a (1) and b (2), along with seven known paxilline-type compounds, were isolated from organic extracts of a new penicillium species (p. thiersii nrrl 28147). the structures of 1 and 2 were determined by analysis of 2d nmr data. thiersinines a and b possess a unique spirocyclic subunit that is unprecedented in previously known compounds of this class. both compounds exhibit potent activity against the fall armyworm (spo ... | 2002 | 12201725 |
| a rapid method for measuring intracellular ph using bcecf-am. | a rapid intracellular ph (ph(i)) measurement method based on initial rate of increase of fluorescence ratio of 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein upon dye addition to a cell suspension in growth medium is reported. a dye transport model that describes dye concentration and fluorescence values in intracellular and extracellular spaces provides the mathematical basis for the approach. experimental results of ammonium chloride challenge response of the two suspension cells, spodoptera ... | 2002 | 12204343 |
| heritability and correlation among some selected morphological traits and their relationship with fall armyworm damage in sweet corn. | fall armyworm [spodoptera frugiperda j. e. smith] (faw) is a serious pest in field corn and sweet corn [zea mays l.] in many parts of the world. sweet corn germplasm with effective levels of resistance to damage by the fall armyworm is needed to transfer resistance to commercial hybrids. the objectives of this study were to estimate heritability and to estimate the correlation among some selected morphological traits and their relationship with faw damage. seven shrunken-2 (sh2) inbred lines and ... | 2002 | 12216502 |
| pharmacological characterisation of 5-hydroxytryptamine-induced contractile effects in the isolated gut of the lepidopteran caterpillar spodoptera frugiperda. | the indolealkylamine 5-hydroxytryptamine (5-ht, 0.1nm-1&mgr;m) caused dose-dependent increases in the number of contractions observed in guts isolated from the caterpillar spodoptera frugiperda. of the 5-ht analogues tested for agonist action, 2-methyl-5-ht (0.1-10&mgr;m) was a full agonist with reduced potency while alpha-methyl-5-ht (0.1-100&mgr;m), 5-carboxamidotryptamine (0.1-100&mgr;m), 5-methoxytryptamine (5-meot) (10nm-10&mgr;m), and tryptamine (1-100&mgr;m) were partial agonists. incubat ... | 2002 | 12770131 |
| structure-activity relationship of contractile effects induced by helicokinins in the isolated gut of the lepidopteran caterpillar spodoptera frugiperda. | the diuretic helicokinins yfspwg-amide (hez ki), vrfspwg-amide (hez kii) and kvkfsawg-amide (hez kiii) are potent contractants of the isolated gut of the caterpillar spodoptera frugiperda at doses ranging from 0.1 to 10nm. in comparison, the pentapeptide fspwg-amide was a full agonist with greatly reduced potency while spwg-amide and pwg-amide were weak partial agonists. substitution of individual amino acids in hez ki with alanine revealed that replacement of the [phenylalanine(2)] residue caus ... | 2002 | 12770134 |
| heritability and correlation among some selected morphological traits and their relationship with fall armyworm damage in sweet corn. | fall armyworm [spodoptera frugiperda j. e. smith] (faw) is a serious pest in field corn and sweet corn [zea mays l.] in many parts of the world. sweet corn germplasm with effective levels of resistance to damage by the fall armyworm is needed to transfer resistance to commercial hybrids. the objectives of this study were to estimate heritability and to estimate the correlation among some selected morphological traits and their relationship with faw damage. seven shrunken-2 (sh2) inbred lines and ... | 2002 | 12658873 |
| purification of dna for the transfection of a spodoptera frugiperda cell line. | spodoptera frugiperda (sf-9) cells have been widely used in baculovirus expression systems, transient gene expression studies and transgenic cell lines. these applications commonly require the transfection of bacterial plasmid dna. one of the most reliable methods of preparing transfection-quality plasmid dna is cesium chloride (cscl) density gradient centrifugation. however, the traditional cscl dna purification is a long and laborious process. we have made a series of modifications to the trad ... | 2002 | 12843704 |
| significant increase in recombinant protein production of a virus-infected sf-9 insect cell culture of low moi under low dissolved oxygen conditions. | spodoptera frugiperda sf-9 insect cells were infected with recombinant autographa californica nuclear polyhedrosis virus at a low multiplicity of infection (moi) (0.1), and the effect of dissolved oxygen (do) on the production of a polyhedrin promoter-driven recombinant protein (beta-galactosidase), intrinsic proteases (carboxyl and cysteine proteases), and the virus was determined. the do concentrations used in the present study were 45%, 25%, 5%, and 1.3% of air saturation. at 5% do the cell g ... | 2002 | 16233329 |
| beauvericin cytotoxicity to the invertebrate cell line sf-9. | the cyclic hexadepsipeptide beauvericin, initially known as a secondary metabolite produced by the entomopathogenic fungus beauveria bassiana and toxic to artemia salina larvae, has been more recently recognized as an important mycotoxin synthesized by a number of fusarium strains, which parasite maize, wheat and rice. therefore, this mycotoxin may enter the food chain, causing yet unknown effects to the health of both domestic animals and humans. the cytotoxic effects of beauvericin on mammalia ... | 2003 | 14617831 |
| stable expression of recombinant human alpha3/4 fucosyltransferase iii in spodoptera frugiperda sf9 cells. | human alpha3/4 fucosyltransferase iii (ft3; ec 2.4.1.65) synthesizes fucosylated glycoconjugates, namely the lewis (le) determinants. ft3 is detected in milk, gastric mucosa, kidney and other organs, but is found in very low amounts in these native tissues. in this work, we describe the expression of a soluble secretory form of ft3 (sft3) in spodoptera frugiperda (sf9) insect cells using a non-lytic vector system. the coding sequence was cloned into the expression vector pib/v5-his-topo which co ... | 2003 | 14636711 |
| growth, viral production and metabolism of a helicoverpa zea cell line in serum-free culture. | insect cell cultures have been extensively utilised for means of production for heterologous proteins and biopesticides. spodoptera frugiperda (sf9) and trichoplusia ni (high fivetrade mark) cell lines have been widely used for the production of recombinant proteins, thus metabolism of these cell lines have been investigated thoroughly over recent years. the helicoverpa zea cell line has potential use for the production of a biopesticide, specifically the helicoverpa armigera single-nucleocapsid ... | 2003 | 19002933 |
| g protein beta(1)gamma (2) subunits purification and their interaction with adenylyl cyclase. | a preliminary study on the interaction of g protein (guanine triphosphate binding protein) beta(1)gamma(2) subunits and their coupled components in cell signal transduction was conducted in vitro. the insect cell lines, sf9 (spodoptera frugiperda) and h5 (trichoplusia ni) were used to express the recombinant protein gbeta(1)gamma(2). the cell membrane containing gbeta(1)gamma(2) was isolated through affinity chromatography column with ni-ntaagarose by fplc method, and the highly purified protein ... | 2003 | 18758712 |
| rice stripe virus 23.9 k protein aggregates and forms inclusion bodies in cultured insect cells and virus-infected plant cells. | the genome of rice stripe virus (rsv, genus tenuivirus) contains seven open reading frames (orfs). little is known about the products of four of these orfs, including the 23.9 k protein encoded by the virus-sense orf of rna3. western blotting revealed that the 23.9 k protein was synthesized in the host plant and also in the planthopper vector of rsv. using a baculovirus vector, the 23.9 k protein was expressed, both unfused and fused with red-shifted green fluorescent protein, in spodoptera frug ... | 2003 | 14579176 |
| vanessa cardui adipokinetic hormone (vanca-akh) in butterflies and a moth. | small neuropeptides of the adipokinetic hormone/red pigment-concentrating hormone (akh/rpch) family regulate energy metabolism in insects. within lepidopterans, the nonapeptide manduca sexta akh (manse-akh) represents a widely occurring akh, whereas the decapeptide helze-hrth (at first isolated from heliothis zea) seems to be restricted to moths. here we show that vanca-akh, a non-amidated undecapeptide which we recently found in the painted lady butterfly, vanessa cardui, is also present in the ... | 2003 | 12781830 |
| existence of catalase-less peroxisomes in sf21 insect cells. | catalase activity, a peroxisomal marker enzyme, was not detectable in any of the subcellular fractions of spodoptera frugiperda (sf) 21 insect cells, although marker enzymes in other organelles were distributed in the fractions in a manner similar to that seen in mammalian cells. when a green fluorescent protein fused with peroxisome targeting signal 1 at the c-terminal (gfp-skl) was expressed in sf21 cells, punctate fluorescent dots were observed in the cytoplasm. the fraction where gfp-skl was ... | 2003 | 12788084 |
| comparative study on the susceptibility of cutworms (lepidoptera: noctuidae) to agrotis segetum nucleopolyhedrovirus and agrotis ipsilon nucleopolyhedrovirus. | the common cutworm (agrotis segetum) and the black cutworm (agrotis ipsilon) are serious soil pests of many vegetable and field crops all over the world. we have demonstrated the cross-infectivity of two baculoviruses, a. segetum nucleopolyhedrovirus (agsenpv) and a. ipsilon nucleopolyhedrovirus (agipnpv) for these two insect pests. the susceptibility of a. segetum to agipnpv was confirmed by dna restriction endonuclease analyses of dna isolated from virus harvested from infected a. segetum larv ... | 2003 | 14615215 |
| antioxidants prevent uv-induced apoptosis by inhibiting mitochondrial cytochrome c release and caspase activation in spodoptera frugiperda (sf9) cells. | oxidative stress has been shown to be associated with apoptosis (programmed cell death) in a number of cell systems. we earlier reported in vitro cultured spodoptera frugiperda (sf9) cells as a model system to study oxidative stress induced apoptosis (j biosciences 24 (1999) 13) and the inhibition of uv-induced apoptosis by the baculovirus antiapoptotic p35 protein that acts as a sink to sequester reactive oxygen species (proc natl acad sci usa 96 (1999) 4838). we now show that uv-induced apopto ... | 2003 | 12798776 |
| differential regulation of two arabidopsis type iii phosphatidylinositol 4-kinase isoforms. a regulatory role for the pleckstrin homology domain. | here, we compare the regulation and localization of the arabidopsis type iii phosphatidylinositol (ptdins) 4-kinases, atpi4kalpha1 and atpi4kbeta1, in spodoptera frugiperda (sf9) insect cells. we also explore the role of the pleckstrin homology (ph) domain in regulating atpi4kalpha1. recombinant kinase activity was found to be differentially sensitive to ptdins-4-phosphate (ptdins4p), the product of the reaction. the specific activity of atpi4kalpha1 was inhibited 70% by 0.5 mm ptdins4p. the eff ... | 2003 | 12805633 |
| isolation and characterization of a human sperm antigen gene h-sp-1. | we isolated and characterized a human sperm antigen gene (h-sp-1) from human testis complementary dna using antiserum against the human sperm membrane. northern blot analysis detected two transcripts (2.3 and 1.1 kb) of the h-sp-1 gene. the 2.3-kb transcript is ubiquitous, whereas the 1.1-kb transcript is specific to the human testis with a high level of expression. determination of the base sequence of h-sp-1 showed a size of 2170 bp and 43.4% homology with human synaptophysin. the base sequenc ... | 2003 | 12846798 |
| synthesis of stereospecifically deuterated 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal) iastereomers and metabolism by a/j mouse lung microsomes and cytochrome p450 2a5. | the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) is a lung carcinogen in mice and rats and is a putative human lung carcinogen. nnk undergoes cytochrome p450-mediated metabolic activation to dna-binding intermediates but is also extensively reduced to 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal) in vivo. because nnal is also tumorigenic, the carcinogenicity of nnk may actually be governed by the metabolic activation of nnal, rather than direct activat ... | 2003 | 12807362 |
| impact of a nucleopolyhedrovirus bioinsecticide and selected synthetic insecticides on the abundance of insect natural enemies on maize in southern mexico. | the impact of commonly used organophosphate (chlorpyrifos, methamidophos), carbamate (carbaryl), and pyrethroid (cypermethrin) insecticides on insect natural enemies was compared with that of a nucleopolyhedrovirus (baculoviridae) of spodoptera frugiperda (j. e. smith) (lepidoptera noctuidae) in maize grown in southern mexico. analyses of the selectv and koppert side effects (iobc) databases on the impact of synthetic insecticides on arthropod natural enemies were used to predict approximately 7 ... | 2003 | 12852601 |
| pathogenesis of autographa californica m nucleopolyhedrovirus in fifth instar spodoptera frugiperda. | we have characterized infection and pathogenesis of an autographa californica m nucleopolyhedrovirus recombinant, acmnpv-hsp70/lacz, carrying the lacz reporter gene, in penultimate (fifth) instar spodoptera frugiperda. bioassays revealed that while <0.1 p.f.u. of budded virus was required to generate an ld(50) by intrahaemocoelic injection, approximately 6000 occlusions were required orally to achieve the same mortality in newly moulted fifth instar (5(0)) larvae. in pathogenesis experiments, 78 ... | 2003 | 12867633 |
| baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway. | in this study we report that the baculovirus p35 anti-apoptotic protein prevents cell death by quenching free radicals at a very upstream step in the apoptotic pathway. mitochondria of activated rat peritoneal macrophages as well as spodoptera frugiperda (sf9) insect cells, following treatment with oxidants, h(2)o(2)/uvb irradiation, release cytochrome c followed by activation of caspase-3. transfection of macrophages/sf9 cells with a construct carrying the p35 gene under the cmv/hsp promoters r ... | 2003 | 12893247 |
| stereospecific deuterium substitution attenuates the tumorigenicity and metabolism of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk). | stereochemical determinants of the tumorigenicity and metabolism of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) were investigated using the stereospecifically deuterated isotopomers (4r)-[4-(2)h(1)]nnk and (4s)-[4-(2)h(1)]nnk. upon ip administration to groups of 20 female a/j mice, nnk and (4s)-[4-(2)h(1)]nnk exhibited similar lung tumorigenicity at three different doses, whereas (4r)-[4-(2)h(1)]nnk was 2-fold less tumorigenic at all three doses. in a pa ... | 2003 | 12807363 |
| protein kinase c delta induces src kinase activity via activation of the protein tyrosine phosphatase ptp alpha. | previously we have shown that protein kinase c (pkc)-mediated reorganization of the actin cytoskeleton in smooth muscle cells is transmitted by the non-receptor tyrosine kinase, src. several authors have described how 12-o-tetradecanoylphorbol-13-acetate (tpa) stimulation of cells results in an increase of src activity, but the mechanism of the pkc-mediated src activation is unknown. using pkc isozymes purified from spodoptera frugiperda insect cells, we show here that pkc is not able to activat ... | 2003 | 12826681 |
| evaluation of oils and microbial pathogens for control of lepidopteran pests of sweet corn in new england. | vegetable and mineral oil, beauveria bassiana (balsamo) and bacillus thuringiensis subsp. kurstaki berliner were evaluated for control of ostrinia nubilalis (hübner) (lepidoptera: pyralidae), helicoverpa zea (boddie) (lepidoptera: noctuidae) and spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae) in sweet corn (zea mays l.). field experiments in maine and massachusetts during 1993 and 1994 evaluated oils and pathogens singly or in combinations, using a single application directly to the ... | 2003 | 14977100 |
| transport of fluorescein methotrexate by multidrug resistance-associated protein 3 in iec-6 cells. | the transport characteristics of fluorescein methotrexate (f-mtx) were studied by using the rat intestinal crypt cell line iec-6. enhanced accumulation of f-mtx at 4 degrees c suggests the existence of an active efflux system. mk-571, an inhibitor of the multidrug resistance-associated protein/atp binding cassette c (mrp/abcc) family, also enhanced the accumulation of f-mtx. transcellular transport of f-mtx from the apical to the basolateral compartment was 2.5 times higher than the opposite dir ... | 2003 | 12909565 |
| analysis of host specificity of two closely related baculoviruses in permissive and nonpermissive cell lines. | the baculoviruses bombyx mori nucleopolyhedrovirus (bmnpv) and autographa californica multinucleocapsid nucleopolyhedrovirus (acmnpv) share about 90% identity at the genomic level but they have non-overlapping host range and show a high degree of host specificity. we have demonstrated here that acmnpv undergoes dna replication and early gene expression in bombyx-derived bmn cells but fails to show very late gene expression or produce budded virion (bv) particles. coinfection with bmnpv supported ... | 2003 | 12727338 |
| sensitivity of invertebrate iridescent virus 6 to organic solvents, detergents, enzymes and temperature treatment. | the sensitivity of invertebrate iridescent virus 6 (iiv-6) to a selection of organic solvents, detergents, enzymes and heat treatment was assayed in spodoptera frugiperda (sf9) cells and by injection of inoculum into larvae of galleria mellonella. in several cases, the degree of sensitivity of the virus depended on the method of assay; cell culture assays indicated greater losses of activity than insect bioassay. iiv-6 was sensitive to chloroform but sensitivity to ether was only detected by cel ... | 2003 | 12573504 |
| activation of vanilloid receptor type i in the endoplasmic reticulum fails to activate store-operated ca2+ entry. | to evaluate interaction of vanilloid receptor type 1 (trpv1) with endogenous ca(2+) signalling mechanisms, trpv1 was expressed in spodoptera frugiperda (sf 9) insect cells using recombinant baculovirus. stimulation of trpv1-expressing cells, but not control sf 9 cells, with resiniferatoxin (rtx), capsaicin or anandamide, produced an increase in cytosolic free ca(2+) concentration ([ca(2+)](i)), with ec(50) values of 166 pm, 24.5 nm and 3.89 microm respectively. in the absence of extracellular ca ... | 2003 | 12608892 |
| maximizing production of estrogen receptor beta with the baculovirus expression system. | steroid hormone/nuclear receptor expression in cultured insect cell lines is routinely driven by a baculovirus vector. an advantage of the baculovirus production of these receptors is that large amounts of functional receptors are obtained for subsequent in vitro studies. most laboratories produce nuclear receptors in spodoptera frugiperda (sf)9 cells. however, no one has determined whether this cell line is optimal for the production of any nuclear receptor. we compared the time course and leve ... | 2003 | 12613256 |
| distribution and diversity of cry genes in native strains of bacillus thuringiensis obtained from different ecosystems from colombia. | colombia is a tropical country located at the north of south america. it is considered to be one of the most important countries in terms of its biodiversity worldwide. one hundred and eight soil samples obtained from agricultural crops and wild ecosystems were evaluated in terms of the presence of bacillus thuringiensis (bt) native strains. one hundred and eight different bt strains were isolated and characterized by the presence of crystal proteins by sds-page and a multiplex pcr with general ... | 2003 | 12623312 |
| field and laboratory evaluations of transgenic cottons expressing one or two bacillus thuringiensis var. kurstaki berliner proteins for management of noctuid (lepidoptera) pests. | field studies were conducted from 1999 to 2001 to evaluate the efficacy of the transgenic cotton, gossypium hirsutum (l.), genotype, bollgard ii (monsanto 15985), which expresses two bacillus thuringiensis berliner (bt) proteins (cry1ac + cry2ab) that are active against lepidopterous pests. bollgard ii was compared with bollgard (dp50b), which expresses only one bt protein (cry1ac), and, in all tests, the conventional variety, dp50, was used as a non-bt control. larval populations of the bollwor ... | 2003 | 12852613 |
| cloning, tissue distribution, and functional studies of a new cytochrome p450 3a subfamily member, cyp3a45, from rainbow trout (oncorhynchus mykiss) intestinal ceca. | in trout and mammals, the major extrahepatic expression site for cyp3a forms is in the intestine. a cdna encoding a new cyp3a subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (rt-pcr), followed by rapid amplification of cdna ends (race)-pcr. in a set of two primers for pcr, a consensus sequence in the highly conserved regions in 17 cyp3a sequences was used for one primer, and the second primer was designed based on adapter sequen ... | 2003 | 12646270 |
| human cyp1a1 allelic variants: baculovirus expression and purification, hydrodynamic, spectral, and catalytical properties and their potency in the formation of all-trans-retinoic acid. | three human cytochrome p450 1a1 (cyp1a1) allelic variants, namely wild-type (cyp1a1.1), cyp1a1.2 (i462v), and cyp1a1.4 (t461n), were expressed as c-terminal his-tagged fusions including a thrombin cleavage site in spodoptera frugiperda insect cells by baculovirus infection. the variants were expressed with 30-90 nmol (1.8-5.4 mg) spectrally active cytochrome p450 per one liter of culture and purified to electrophoretic homogeneity by ni-agarose chromatography. the recombinant variants were struc ... | 2003 | 12699690 |
| molecular characterisation of cdnas from the fall armyworm spodoptera frugiperda encoding manduca sexta allatotropin and allatostatin preprohormone peptides. | allatotropin (at) is a 13-residue amidated neuropeptide, first isolated from pharate adult heads of the tobacco hornworm, manduca sexta (manse-at), which strongly stimulates the biosynthesis of juvenile hormones (jh) in the corpora allata (ca) of adult moths. in spodoptera frugiperda, a cdna that encodes 134 amino acids, including an at peptide, has been cloned. the s. frugiperda allatotropin mature peptide (spofr-at) [gfknvemmtargfa] is identical to that isolated from m. sexta. the basic organi ... | 2003 | 12706626 |
| expression and purification of recombinant kaposi's sarcoma-associated herpesvirus dna polymerase using a baculovirus vector system. | the dna polymerase (pol) of kaposi's sarcoma-associated herpesvirus (kshv) is essential for viral dna replication and, thus, may be considered as a viable target for anti-kshv therapeutics. to produce large quantities of homogeneous and pure pol required for high-throughput screening (hts) for inhibitors, we generated a recombinant baculovirus vector encoding a hexahistidine (his6)-tagged pol and infected spodoptera frugiperda sf-9 insect cells. high expression of recombinant pol (rpol) was achi ... | 2003 | 12729724 |
| analysis of two d1-like dopamine receptors from the honey bee apis mellifera reveals agonist-independent activity. | dopamine is found in many invertebrate organisms, including insects, however, the mechanisms through which this amine operates remain unclear. we have expressed two dopamine receptors cloned from honey bee (amdop1 and amdop2) in insect cells (spodoptera frugiperda), and compared their pharmacology directly using production of camp as a functional assay. in each assay, amdop1 receptors required lower concentrations of dopamine and 6,7-adtn for maximal activation than amdop2 receptors. conversely, ... | 2003 | 12750008 |