Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| [determination of lc 90 and lt 90 of ibcb66 beauveria bassiana (ascomycetes: clavicipitaceae) isolate for rhipicephalus (boophilus) microplus (acari: ixodidae) control]. | the objective of this research was to evaluate the pathogenicity and the virulence of the ibcb66 isolate of beauveria bassiana on infected larvae of rhipicephalus (boophilus) microplus. the ibcb66 fungus strain was used as standard isolates of b. bassiana against r. (b.) microplus larvae. the larval bioassay tests using the ibcb66 isolate were carried out to determine the (lethal concentration) lc50, lc90, (lethal time) lt50 and lt90. the ibcb66 fungus strain was tested at six different concentr ... | 2009 | 20040188 |
| [in vitro analysis of amitraz efficacy against rhipicephalus (boophilus) microplus (canestrini, 1887) populations of southern region of rio grande do sul state]. | commercial formulation of the amitraz is the most used acaricide in the southern region of rio grande do sul state, however scientific data about the efficacy of these products in this region are sparse. the aim of the present study was to evaluate the frequency of r. (b.) microplus populations resistant to amitraz acaricides in the southern region of rio grande do sul state. so, the efficacies of five commercial products containing amitraz were compared. results showed that the efficacy of amit ... | 2009 | 20040192 |
| [compatibility of beauveria bassiana (ascomycetes: clavicipitaceae) with chemicals acaricides used in the control of cattle tick]. | the purpose of the present study was to assess compatibility between ibcb66 and ibcb21 isolates of beauveria bassiana and acaricides: flumethrin+coumaphos, deltamethrin, dichlorvos+cypermethrin, dichlorvos+chlorpyrifos, cypermethrin high cis, dichlorvos+cypermethrin high cis, cypermethrin and amitraz, utilized on the control of rhipicephalus (boophilus) microplus in our country. the effect of commercial products on the isolates was assayed according to observation of vegetative growth, conidia p ... | 2009 | 20040194 |
| prevalence of equine piroplasmosis and its association with tick infestation in the state of são paulo, brazil. | serum samples were collected from 582 horses from 40 stud farms in the state of são paulo and tick (acari: ixodidae) infestations were evaluated on them. serum samples were subjected to the complement fixation test (cft) and a competitive inhibition elisa (celisa) for babesia caballi and theileria equi. logistic regression analyses were performed to construct multivariate models that could explain the dependent variable (horses positive for b. caballi or t. equi) as a function of the independent ... | 2009 | 20040201 |
| comparison of efficiency and reproductive aptitude indexes between a reference and field strains of the cattle tick rhipicephalus (boophilus) microplus, in sinaloa, mexico. | the objective of this work was to study the reproductive behavior of two strains of r. (b.) microplus, one wild-caught (native) and one reference strain (media joya) for two years, measuring the reproductive efficiency index (rei) and reproductive aptitude index (rai). engorged ticks from each strain were collected monthly from february 2002 to february 2004. these were weighed and kept in the laboratory until egg-laying. oogenic masses were individually weighed and incubated until larvae emerge ... | 2009 | 20040202 |
| [grazing systems, rotenone and parasites control in crossbred calves: effect on live weight gain and on parasites burdens]. | practices for endo and ectoparasite control in beef cattle were evaluated in two independent experiments. first, the effects of rotenone on rhipicephalus (boophilus) microplus ticks were evaluated in vitro and in experimentally infected calves. in the second trial, the effects of grazing systems associated with endo and ectoparasite treatments on parasite burden and weight gain of naturally parasited animals were evaluated. rotenone showed acaricide action on larvae and engorged ticks during in ... | 2009 | 20040207 |
| [plant extracts in control of rhipicephalus (boophilus) microplus (canestrini, 1887) (acari: ixodidae) in laboratory]. | plant extracts were studied to evaluate its efficiency in the control of rhipicephalus (boophilus) microplus (canestrini, 1887) in laboratory. engorged female ticks were collected from the cattle, kept in petri dishes. organic alcoholic extracts 2% (weight/volume) were used: seeds of annona muricata l. (annonaceae) (soursop); flowers of syzygium malaccensis (l.) (myrtaceae) (iamb), leaves of cymbopogon citratus (dc.) stapf (poaceae) (holy grass), leaves of azadirachta indica a. juss (meliaceae) ... | 2009 | 20040208 |
| identification of a synthetic peptide inducing cross-reactive antibodies binding to rhipicephalus (boophilus) decoloratus, rhipicephalus (boophilus) microplus, hyalomma anatolicum anatolicum and rhipicephalus appendiculatus bm86 homologues. | the bm86 antigen, originally identified in rhipicephalus (boophilus) microplus, is the basis of the only commercialized anti-tick vaccine. the long-term goal of our study is to improve bm86 based vaccines by induction of high levels of tick gut binding antibodies that are also cross-reactive with a range of bm86 homologues expressed in other important tick species. here we have used a bd86 derived synthetic peptide, bd86-3, to raise a series of mouse monoclonal antibodies. one of these mabs, nam ... | 2009 | 19808026 |
| immune response of bovines stimulated by synthetic vaccine sbm7462 against rhipicephalus (boophilus) microplus. | ten-month-old calves bos taurus taurus were immunized with three doses of sbm7462 with saponin as an adjuvant at 30-day intervals and were evaluated for igg isotypes, phenotype circulating lymphocytes and changes in the lymph nodes (ln). sbm7462 stimulated the production of predominantly igg1-isotype igg antibodies. the lymph nodes exhibited activation at the seventh day after the first immunization, with areas of paracortical and interfollicular hyperplasia and the early formation of germinal c ... | 2009 | 19811877 |
| adulticidal and larvicidal efficacy of some medicinal plant extracts against tick, fluke and mosquitoes. | the adulticidal and larvicidal effect of indigenous plant extracts were investigated against the adult cattle tick haemaphysalis bispinosa neumann, 1897 (acarina: ixodidae), sheep fluke paramphistomum cervi zeder, 1790 (digenea: paramphistomatidae), fourth instar larvae of malaria vector, anopheles subpictus grassi and japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae). the aim of this study was to evaluate the toxic effect of leaf hexane, chloroform, ethyl acetate, ... | 2009 | 19819626 |
| two novel neuropeptides in innervation of the salivary glands of the black-legged tick, ixodes scapularis: myoinhibitory peptide and sifamide. | the peptidergic signaling system is an ancient cell-cell communication mechanism that is involved in numerous behavioral and physiological events in multicellular organisms. we identified two novel neuropeptides in the neuronal projections innervating the salivary glands of the black-legged tick, ixodes scapularis (say, 1821). myoinhibitory peptide (mip) and sifamide immunoreactivities were colocalized in the protocerebral cells and their projections terminating on specific cells of salivary gla ... | 2009 | 19824085 |
| a survey of rhipicephalus microplus populations for mutations associated with pyrethroid resistance. | mutations associated with pyrethroid resistance were found in mexican strains of rhipicephalus microplus (canestrini). a mutation in the sodium channel gene was reported in strains highly resistant to permethrin and another mutation in an esterase gene in a strain that shows moderate resistance to the same pesticide. methods based on the melting temperature difference of amplified allele-specific dna fragments were developed that can detect these mutations rapidly in individual larvae. when thes ... | 2009 | 19253657 |
| biostable agonists that match or exceed activity of native insect kinins on recombinant arthropod gpcrs. | the multifunctional arthropod 'insect kinins' share the evolutionarily conserved c-terminal pentapeptide motif phe-x(1)-x(2)-trp-gly-nh(2), where x(1)=his, asn, ser, or tyr and x(2)=ser, pro, or ala. insect kinins regulate diuresis in many species of insects. compounds with similar biological activity could be exploited for the control of arthropod pest populations such as the mosquito aedes aegypti (l.) and the southern cattle tick rhipicephalus (boophilus) microplus (canestrini), vectors of hu ... | 2009 | 18983996 |
| structure and mode of action of microplusin, a copper ii-chelating antimicrobial peptide from the cattle tick rhipicephalus (boophilus) microplus. | microplusin, a rhipicephalus (boophilus) microplus antimicrobial peptide (amp) is the first fully characterized member of a new family of cysteine-rich amps with histidine-rich regions at the n and c termini. in the tick, microplusin belongs to the arsenal of innate defense molecules active against bacteria and fungi. here we describe the nmr solution structure of microplusin and demonstrate that the protein binds copper ii and iron ii. structured as a single alpha-helical globular domain, micro ... | 2009 | 19828445 |
| protective efficacy of bacterial membranes containing surface-exposed bm95 antigenic peptides for the control of cattle tick infestations. | the rhipicephalus (boophilus) microplus bm86 and bm95 glycoproteins are homologous proteins that protect cattle against tick infestations. in this study, we demonstrated that the recombinant chimeric protein comprising tick bm95 immunogenic peptides fused to the a. marginale msp1a n-terminal region for presentation on the escherichia coli membrane was protective against r. microplus infestations in rabbits. this system provides a novel and simple approach for the production of tick protective an ... | 2009 | 19835826 |
| phylogeographic analysis reveals association of tick-borne pathogen, anaplasma marginale, msp1a sequences with ecological traits affecting tick vector performance. | the tick-borne pathogen anaplasma marginale, which is endemic worldwide, is the type species of the genus anaplasma (rickettsiales: anaplasmataceae). rhipicephalus (boophilus) microplus is the most important tick vector of a. marginale in tropical and subtropical regions of the world. despite extensive characterization of the genetic diversity in a. marginale geographic strains using major surface protein sequences, little is known about the biogeography and evolution of a. marginale and other a ... | 2009 | 19723295 |
| ticks on domestic animals in pernambuco, northeastern brazil. | the objective of this article was to discuss some aspects of ticks associated with domestic animals in the state of pernambuco, northeastern brazil, based on a literature review and present new data obtained from recent tick collections carried out in this northeastern brazilian state. from august 2007 to june 2008, 1,405 ticks were collected and five species were identified: amblyomma cajennense (fabricius, 1787), amblyomma ovale koch, 1844, dermacentor nitens neumann, 1897, rhipicephalus (boop ... | 2009 | 19772771 |
| a survey of ectoparasitic arthropods on domestic animals in tak province, thailand. | in july 2008 a survey of ectoparasites on domestic animals was conducted in the royal thai army areas of operation along the thai-myanmar border, tak province, thailand. eleven different ectoparasites were collected: two species of hard ticks (ixodidae), three species of fleas (siphonaptera) and 6 species of sucking or chewing lice (2 species each in the suborders anoplura, ischnocera and amblycera) were collected. domestic dogs (canis lupusfamiliaris) (n = 94) were found infested with 2 species ... | 2009 | 19842427 |
| hemoglobin digestion in blood-feeding ticks: mapping a multipeptidase pathway by functional proteomics. | hemoglobin digestion is an essential process for blood-feeding parasites. using chemical tools, we deconvoluted the intracellular hemoglobinolytic cascade in the tick ixodes ricinus, a vector of lyme disease and tick-borne encephalitis. in tick gut tissue, a network of peptidases was demonstrated through imaging with specific activity-based probes and activity profiling with peptidic substrates and inhibitors. this peptidase network is induced upon blood feeding and degrades hemoglobin at acidic ... | 2009 | 19875079 |
| toxicity of geraniol solution in vitro to the poultry red mite, dermanyssus galinae. | geraniol is a bioactive component found in palmarosa essential oil which has previously demonstrated good repellence to cattle ticks, mosquitoes and sand flies. the toxicity of geraniol to dermanyssus gallinae, the most economically deleterious ectoparasite of laying hens in europe, was studied in vitro in the laboratory. exposure of d. gallinae to 0.5, 1 and 2% concentrations of geraniol (equating approximately to 0.02, 0.04 and 0.07 mg/cm2 geraniol, respectively) over a period of 24 hours resu ... | 2009 | 20092065 |
| differential immunoproteomics enables identification of metarhizium anisopliae proteins related to rhipicephalus microplus infection. | differential immunoproteomics was applied to identify proteins secreted by metarhizium anisopliae induced by the rhipicephalus microplus cuticle. in addition, igg anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. lc-ms/ms of differentially secreted proteins led to the identification of proteases (carboxypeptidase and pr1a), chitinase, carboxylic acid transport and proline-rich protein. differential immunoproteomics ... | 2009 | 19800970 |
| the comparative prevalence of five ixodid tick species infesting cattle and goats in maputo province, mozambique. | this study compares the prevalence of ixodid tick species on cattle and goats in maputo province. adult ticks as well as the nymphs of three species, and only the adults of two species were collected from sets of five cattle at 21 localities throughout the province and compared with those collected from similar sets of goats at the same places. amblyomma hebraeum adults and/or nymphs were present on cattle and on goats at all 21 localities, and 90 cattle and 22 goats were infested with adult tic ... | 2009 | 20698440 |
| trafficking of heme and porphyrins in metazoa. | 2009 | 19764719 | |
| species composition and geographic distribution of ticks infesting cattle, goats and dogs in a temperate and in a subtropical region of south-east africa. | the species and distribution of ticks infesting cattle, goats and dogs in the eastern region of the eastern cape province, south africa and maputo province, mozambique were determined from collections made from these animals at 72 localities in the former region and 30 in the latter. eleven ixodid and one argasid species were recovered in the eastern cape province and 15 ixodid species in maputo province. the most common ticks infesting cattle and goats in both provinces were amblyomma hebraeum, ... | 2009 | 21105593 |
| tick-derived kunitz-type inhibitors as antihemostatic factors. | endogenous kunitz-type inhibitors target a large number of serine proteinases, including coagulation factors viia and xa, but not thrombin. by contrast, several two-domain kunitz inhibitors of this major procoagulant proteinase have been isolated from both soft ticks (e.g., ornithodorin from ornithodoros moubata) and hard ticks (e.g., boophilin from rhipicephalus (boophilus) microplus). surprisingly, these anticoagulants do not follow the canonical mechanism of proteinase inhibition. instead, th ... | 2009 | 19631744 |
| microarray analysis of gene expression changes in feeding female and male lone star ticks, amblyomma americanum (l). | a collection of est clones from female tick amblyomma americanum salivary glands was hybridized to rna from different feeding stages of female tick salivary glands and from unfed or feeding adult male ticks. in the female ticks, the expression patterns changed dramatically upon starting feeding, then changed again towards the end of feeding. on beginning feeding, genes possibly involved in survival on the host increased in expression as did many housekeeping genes. as feeding progressed, some of ... | 2009 | 19514082 |
| ticks on birds in a forest fragment of brazilian cerrado (savanna) in the municipality of uberlândia, state of minas gerais, brazil. | this is a report of tick species, parasite prevalence and infestation intensity of birds in a forest fragment (18° 56' 57'' s and 48° 12' 14'' w) within the brazilian cerrado (savanna), in the municipality of uberlândia, state of minas gerais, brazil. a total of 162 birds from 26 species were captured. one adult tick, 296 larvae and 67 nymphs were found on passerine birds. of these, it was identified 31 larvae and 27 nymphs of amblyomma longirostre, 17 nymphs of a. nodosum, one a. cajennense lar ... | 2010 | 21184702 |
| bartonella and babesia infections in cattle and their ticks in taiwan. | bartonella and babesia infections and the association with cattle breed and age as well as tick species infesting selected cattle herds in taiwan were investigated. blood samples were collected from 518 dairy cows and 59 beef cattle on 14 farms and 415 ticks were collected from these animals or in a field. bartonella and babesia species were isolated and/or detected in the cattle blood samples and from a selected subset (n=254) of the ticks either by culture or dna extraction, pcr testing and dn ... | 2010 | 21194750 |
| epidemiological analysis of tick-borne diseases in zambia. | tick-borne diseases are a constraint to livestock production in many developing countries as they cause high morbidity and mortality, which results in decreased production of meat, milk and other livestock by-products. the most important tick-borne diseases of livestock in sub-saharan africa are east coast fever (caused by theileria parva), babesiosis (caused by babesia bigemina and b. bovis), anaplasmosis (caused by anaplasma marginale) and heartwater (caused by ehrlichia ruminantium). despite ... | 2010 | 21106294 |
| megaselia scalaris reared on rhipicephalus (boophilus) microplus laboratory cultures. | different laboratory cultures of the acarine tick rhipicephalus (boophilus) microplus (canestrini, 1888) (ixodida: ixodidae) were infested by small megaselia scalaris (loew, 1866) (diptera: phoridae) flies. larvae of this species exhibited opportunistic parasitism predominantly on engorged female ticks, causing severe damage to their cuticle through which the flies were able to reach r. microplus internal organs, on which they fed until developing into pupae in the tick's remains. the flies were ... | 2010 | 21143490 |
| two initial vaccinations with the bm86-based gavacplus vaccine against rhipicephalus (boophilus) microplus induce similar reproductive suppression to three initial vaccinations under production conditions. | the cattle tick, rhipicephalus (boophilus) microplus, affects livestock production in many regions of the world. up to now, the widespread use of chemical acaricides has led to the selection of acaricide-resistant ticks and to environmental contamination. gavacplus is a subunit vaccine based on the recombinant bm86 tick antigen expressed in yeast, capable to control infestations of r. microplus under controlled and production conditions. the vaccine constitutes the core element of broad control ... | 2010 | 20846415 |
| in vitro acaricidal effect of tannin-rich plants against the cattle tick rhipicephalus (boophilus) microplus (acari: ixodidae). | the objectives of this study were to evaluate the in vitro acaricidal effects of lyophilized extracts of four tannin rich plants (acacia pennatula, piscidia piscipula, leucaena leucocephala and lysiloma latisiliquum) against diverse stages of rhipicephalus (boophilus) microplus, and to asses whether tannins were involved in the acaricidal effect using polyethylene glycol (peg) to block tannins. larval immersion (lit) and adult immersion (ait) tests were used to evaluate the acaricidal effect of ... | 2010 | 20947253 |
| metarhizium anisopliae lipolytic activity plays a pivotal role in rhipicephalus (boophilus) microplus infection. | lipases secreted by metarhizium anisopliae, an important biological control agent, could potentially be involved in the host infection process. here, we present the activity profile during the host infection process and the effect of lipase activity inhibitor ebelactone b on infection. the previous treatment of spores with lipase activity inhibitor, ebelactone b, completely inhibited lipolytic activity and prevented the infection of the rhipicephalus (boophilus) microplus host. the results herei ... | 2010 | 20965056 |
| acaricidal efficacy against cattle ticks and acute oral toxicity of lippia javanica (burm f.) spreng. | in search for low-cost, safe and environmentally benign plant-based alternatives to commercial pesticides, the efficacy of lippia javanica aqueous leaf extracts in controlling ticks on cattle, acute oral toxicity in mice and phytochemistry were evaluated. l. javanica aqueous leaf extracts at 10% and 20% w/v were effective at controlling cattle ticks but not as good as an amitraz-based acaricide tickbuster. however, they can provide an effective tick control option where synthetic products are un ... | 2010 | 20978842 |
| identification of a mutation in the para-sodium channel gene of the cattle tick rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin. | a mutation in the domain ii s4-5 linker region of the para-sodium channel gene has been associated previously with synthetic pyrethroid (sp) resistance in the cattle tick (rhipicephalus microplus) in australia. this is a c→a mutation at nucleotide position 190, which results in a leucine to isoleucine amino acid substitution (l64i). in a survey of 15 cattle tick populations with known sp resistance status, sourced from queensland and new south wales in australia, there was a strong relationship ... | 2010 | 20708620 |
| molecular cloning of a small heat shock protein (shspii) from the cattle tick rhipicephalus (boophilus) annulatus salivary gland. | immunoscreening of a cdna expression library of the rhipicephalus (boophilus) annulatus tick with purified rabbit anti-r annulatus salivary glands antigens polyclonal antibodies led to the identification of a 661bp sequence. the sequence includes an open reading frame of 543bp encoding a protein of 180 amino acids with calculated molecular weight of 20.51kda, isoelectric point of 9.071 and with no signal sequence. comparison of the deduced amino acids with protein data bank showed that the ident ... | 2010 | 20723560 |
| bm86 midgut protein sequence variation in south texas cattle fever ticks. | abstract: | 2010 | 21047431 |
| rhipicephalus microplus salivary gland molecules induce differential cd86 expression in murine macrophages. | abstract: | 2010 | 21054882 |
| potential synergistic effect of melia azedarach fruit extract and beauveria bassiana in the control of rhipicephalus (boophilus) microplus (acari: ixodidae) in cattle infestations. | the use of a concentrate emulsion of melia azedarach green fruits and a suspension of the fungus beauveria bassiana was evaluated in the control of rhipicephalus microplus on artificially infested cattle. the evaluation was conducted following the protocol established by the brazilian agriculture ministry. five groups of 4 or 5 animals were allocated to one of the following treatments: emulsion concentrate of m. azedarach at 0.25% (t azed 0.25%), emulsion concentrate of m. azedarach at 0.5% (t a ... | 2010 | 21055878 |
| evaluation of bacillus thuringiensis pathogenicity for a strain of the tick, rhipicephalus microplus, resistant to chemical pesticides. | the pathogenicity of four native strains of bacillus thuringiensis against rhipicephalus (boophilus) microplus (canestrine) (acari: ixodidae) was evaluated. a r. microplus strain that is resistant to organophosphates, pyrethroids, and amidines, was used in this study. adult r. microplus females were bioassayed using the immersion test of drummond against 60 b. thuringiensis strains. four strains, gp123, gp138, gp130, and gp140, were found to be toxic. for the immersion test, the total protein co ... | 2010 | 21062139 |
| transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite psoroptes ovis. | infestation of ovine skin with the ectoparasitic mite psoroptes ovis results in a rapid cutaneous immune response, leading to the crusted skin lesions characteristic of sheep scab. little is known regarding the mechanisms by which such a profound inflammatory response is instigated and to identify novel vaccine and drug targets a better understanding of the host-parasite relationship is essential. the main objective of this study was to perform a combined network and pathway analysis of the in v ... | 2010 | 21067579 |
| the rhipicephalus (boophilus) microplus bm86 gene plays a critical role in the fitness of ticks fed on cattle during acute babesia bovis infection. | abstract: | 2010 | 21092112 |
| the application of phage-displayed peptide libraries to ligand detection in eggs and larvae of rhipicephalus (boophilus) microplus. | the cattle tick, rhipicephalus (boophilus) microplus is an ectoparasite of cattle and is one of the major limiting factors in the use of bos taurus cattle in tropical and subtropical countries. current control relies heavily on chemotherapy with synthetic acaricides, which is threatened by the development of resistant tick populations. novel approaches to target discovery in cattle ticks may provide alternative strategies for the control of these parasites. the value of phage-display technology ... | 2010 | 20609525 |
| spread of parasites transported with their hosts: case study of two species of cattle tick. | like all parasites, ticks can be spread easily along with their hosts. ticks are obligate parasites of vertebrates, to which they attach themselves for varying periods of time, and are well-adapted to this mode of transport. once the transport stage is complete and they have detached at destination, they are also able to wait several months for the arrival of a new host on which they will continue their life cycle. this leads to the establishment of a secondary tick population. two tropical catt ... | 2010 | 20617654 |
| effects of urea on the cattle tick rhipicephalus (boophilus) microplus (acari: ixodidae). | this study aimed at evaluating the effects of urea on rhipicephalus (boophilus) microplus. the experiment was divided into two stages. in stage i, brachiaria brizantha was placed into 30 pots, each with an area of 18 cm(2).these were divided into three groups of ten pots each: g1 non-treated control group, g2 treated with 15 g of urea per pot and g3 treated with 15 g of urea+10% of ammonium sulphate. three engorged female ticks were placed in each pot and then 1.8l of water were added. in the se ... | 2010 | 20855169 |
| identification of a dieldrin resistance-associated mutation in rhipicephalus (boophilus) microplus (acari: ixodidae). | the southern cattle tick, rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae), is a major vector of tick fever organisms affecting cattle in many parts of the world, including australia, africa, and south america. control of the southern cattle tick through acaricide use is an important approach in disease management. resistance has emerged to many of the acaricides currently and previously used, including the cyclodienes. although cyclodiene resistance mechanisms have been charac ... | 2010 | 20857747 |
| laboratory evaluation of verbutin as a synergist of acaricides against larvae of rhipicephalus (boophilus) microplus (acari: ixodidae). | synergistic effects of verbutin, a member of aryl alkynyl derivatives, to three commonly used acaricides were evaluated with the modified food and agricultural organization larval packet test (fao-lpt) against both susceptible and resistant strains of the southern cattle tick, rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae). these tick strains demonstrated various levels of resistance to coumaphos (2.5-8.2x), permethrin (57.9-711.7x), and amitraz (3.5-177.5x). verbutin alone w ... | 2010 | 20857748 |
| acaricidal effect and chemical composition of essential oils extracted from cuminum cyminum, pimenta dioica and ocimum basilicum against the cattle tick rhipicephalus (boophilus) microplus (acari: ixodidae). | acaricidal activity of essential oils extracted from cumin seeds (cuminum cyminum), allspice berries (pimenta dioica) and basil leaves (ocimum basilicum) were tested on 10-day-old rhipicephalus (boophilus) microplus tick larvae using the lpt. two-fold dilutions of the three essential oils were tested from a starting dilution of 20% down to 1.25%. results showed a high toxicological effect for cumin, producing 100% mortality in all tested concentrations on r. microplus larvae. similarly, allspice ... | 2010 | 20865426 |
| in vitro and in vivo efficacy of acorus calamus extract against rhipicephalus (boophilus) microplus. | to develop a environment friendly control measure against cattle tick, rhipicephalus (boophilus) microplus, medicinally important plants were identified and extracts were prepared. twelve 95% ethanolic, thirteen 50% hydroethanolic and nine hot water extracts were prepared and tested against laboratory reared homogenous colony of r. (b.) microplus. amongst the 34 extracts, 26 extracts showed no mortality within 72 h of application while 12.0 ± 4.9% to 35.0 ± 9.6% mortality of treated ticks was re ... | 2010 | 20886235 |
| lectins: production and practical applications. | lectins are proteins found in a diversity of organisms. they possess the ability to agglutinate erythrocytes with known carbohydrate specificity since they have at least one non-catalytic domain that binds reversibly to specific monosaccharides or oligosaccharides. this articles aims to review the production and practical applications of lectins. lectins are isolated from their natural sources by chromatographic procedures or produced by recombinant dna technology. the yields of animal lectins a ... | 2010 | 20890754 |
| evaluation of medicinal plant extracts against ticks and fluke. | the present study was based on assessments of the antiparasitic activities to determine the efficacies of leaf hexane, chloroform, ethyl acetate, acetone and methanol extracts of aegle marmelos (linn.) correa ex roxb, andrographis lineata wallich ex nees., andrographis paniculata (burm.f.) wallich ex nees., cocculus hirsutus (l.) diels, eclipta prostrata l., and tagetes erecta l. against the adult cattle tick haemaphysalis bispinosa neumann 1897 (acarina: ixodidae), the larvae of rhipicephalus ( ... | 2010 | 20922419 |
| differential expression of genes in resistant versus susceptible gyr x holstein cattle challenged with the tick rhipicephalus (boophilus) microplus. | the bovine tick rhipicephalus (boophilus) microplus causes major losses in cattle production systems in tropical regions. bos indicus breeds are more resistant to ticks than b. taurus breeds. resistance genes could be an alternative to control this parasite. we examined the pattern of gene expression of three calcium-binding-protein genes: translationally controlled tumor protein 1 (tpt1), allergen bos d3 (s100a7), calcium channel protein transient receptor potential vanilloid 6 (trpv6), and the ... | 2010 | 20927715 |
| survey of rhipicephalus microplus resistance to ivermectin at cattle farms with history of macrocyclic lactones use in yucatan, mexico. | engorged females of rhipicephalus microplus were collected from 30 cattle farms in yucatan, mexico to evaluate ivermectin resistance. the larval progeny of each tick sample were produced in laboratory and evaluated using the larval immersion test to obtain the larval mortality. concentration-mortality data were subjected to probit analysis to generate lethal concentrations (lc). resistance ratio (rr) of each tick sample was calculated by dividing its lc with that of an ivermectin-susceptible str ... | 2010 | 20570047 |
| a novel amino acid substitution in the para-sodium channel gene in rhipicephalus microplus (acari: ixodidae) associated with knockdown resistance. | resistance acquired by the tick rhipicephalus microplus (canestrini) to different types of ixodicides in mexico has had a negative impact on national and local livestock, mainly due to the transmission of diseases such as babesiosis and anaplasmosis, among others. the technique used for the diagnosis of resistance was that in the bioassays noted in the norma oficial mexicana (nom-006-zoo-1994). the purpose of this investigation was the determination of resistance to pyrethroids through isoleucin ... | 2010 | 20585841 |
| swift sympatric adaptation of a species of cattle tick to a new deer host in new caledonia. | the occurrence and frequency of sympatric speciation in natural systems continue to be hotly debated issues in evolutionary biology. this might reflect the timescale over which evolution occurs resulting in there being few compelling observations of the phenomenon (lake fishes, phytophagous insects and island trees). despite predictions, few examples of sympatric speciation have been recorded in animal parasites, at least widely accepted as such. here we show that, in new caledonia, the monophas ... | 2010 | 20601171 |
| efficiency of lecanicillium lecanii to control the tick rhipicephalus microplus. | rhipicephalus microplus, known as the cattle tick, causes serious economic losses in the brazilian cattle industry each year. traditional parasite control is primarily based on the use of chemical acaricides, which unfortunately have many negative side effects. biological control is seen as a promising alternative to chemical acaricide use. this study evaluates the entomopathogenic fungus lecanicillium lecanii for effectiveness in controlling engorged females, eggs, and larvae of r. microplus. c ... | 2010 | 20605335 |
| recombinant peptides as new immunogens for the control of the bovine tick, rhipicephalus (boophilus) microplus. | a candidate vaccine that effectively controls rhipicephalus microplus ticks in livestock is an attractive strategy in bovine management because of the expensive and labor-intensive treatment of animals with acaricides. however, the complex nature of ectoparasites has imposed restrictions on the development of such a vaccine, and its future efficacy is still under debate. using phage display technology, we developed specific immunogens that mimic r. microplus tick antigens and were successfully v ... | 2010 | 20488622 |
| invasion of mosquito salivary glands by malaria parasites: prerequisites and defense strategies. | the interplay between vector and pathogen is essential for vector-borne disease transmission. dissecting the molecular basis of refractoriness of some vectors may pave the way to novel disease control mechanisms. a pathogen often needs to overcome several physical barriers, such as the peritrophic matrix, midgut epithelium and salivary glands. additionally, the arthropod vector elicites immune responses that can severely limit transmission success. one important step in the transmission of most ... | 2010 | 20621627 |
| comparative microarray analysis of rhipicephalus (boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on bos indicus and bos taurus cattle. | rhipicephalus (boophilus) microplus is an obligate blood feeder which is host specific to cattle. existing knowledge pertaining to the host or host breed effects on tick transcript expression profiles during the tick - host interaction is poor. | 2010 | 20637126 |
| bm86 homologues and novel ataq proteins with multiple epidermal growth factor (egf)-like domains from hard and soft ticks. | tick control on livestock relies principally on the use of acaricides but the development of acaricide resistance and concerns for environmental pollution underscore the need for alternative control methods, for instance through the use of anti-tick vaccines. two commercial vaccines based on the recombinant bm86 protein from rhipicephalus (boophilus) microplus ticks were developed. partial protection of the bm86 vaccine against other rhipicephalus (boophilus) and hyalomma tick species suggests t ... | 2010 | 20647015 |
| an insight into the sialotranscriptome of the brown dog tick, rhipicephalus sanguineus. | rhipicephalus sanguineus, known as the brown dog tick, is a common ectoparasite of domestic dogs and can be found worldwide. r.sanguineus is recognized as the primary vector of the etiological agent of canine monocytic ehrlichiosis and canine babesiosis. here we present the first description of a r. sanguineus salivary gland transcriptome by the production and analysis of 2,034 expressed sequence tags (est) from two cdna libraries, one consctructed using mrna from dissected salivary glands from ... | 2010 | 20650005 |
| endophytic and entomopathogenic strains of beauveria sp to control the bovine tick rhipicephalus (boophilus) microplus. | pathogenicity of strains of the entomopathogenic fungus beauveria bassiana and endophytic strains of beauveria sp against the bovine tick rhipicephalus (boophilus) microplus was tested in laboratory bioassays and under field conditions. suspensions containing 10(5), 10(7) and 10(9) conidia/ml were prepared of each fungal strain for laboratory bioassays. the ticks were maintained at 28 degrees c, 90 +/- 5% relative humidity, and the following variables were evaluated: initial female weight, egg w ... | 2010 | 20662157 |
| characterization of proteinases from the midgut of rhipicephalus (boophilus) microplus involved in the generation of antimicrobial peptides. | abstract: | 2010 | 20663211 |
| amblyomma imitator ticks as vectors of rickettsia rickettsii, mexico. | real-time pcr of amblyomma imitator tick egg masses obtained in nuevo leon state, mexico, identified a rickettsia species. sequence analyses of 17-kd common antigen and outer membrane protein a and b gene fragments showed to it to be r. rickettsii, which suggested a potential new vector for this bacterium. | 2010 | 20678325 |
| pyrethroid resistance in aedes aegypti from grand cayman. | the grand cayman population of aedes aegypti is highly resistant to ddt and pyrethroid insecticides. glutathione transferase, cytochrome p450, and esterase levels were increased in the grand cayman population relative to a susceptible laboratory strain, but synergist studies did not implicate elevated insecticide detoxification as a major cause of resistance. the role of target site resistance was therefore investigated. two substitutions in the voltage-gated sodium channel were identified, v101 ... | 2010 | 20682868 |
| acaricide resistance mechanisms in the two-spotted spider mite tetranychus urticae and other important acari: a review. | the two-spotted spider mite tetranychus urticae koch is one of the economically most important pests in a wide range of outdoor and protected crops worldwide. its control has been and still is largely based on the use of insecticides and acaricides. however, due to its short life cycle, abundant progeny and arrhenotokous reproduction, it is able to develop resistance to these compounds very rapidly. as a consequence, it has the dubious reputation to be the"most resistant species" in terms of the ... | 2010 | 20685616 |
| diagnoses of fipronil resistance in brazilian cattle ticks (rhipicephalus (boophilus) microplus) using in vitro larval bioassays. | fipronil is a phenylpyrazolic insecticide that is widely used in agriculture and has been recently used to control the cattle tick, rhipicephalus (boophilus) microplus. because of the serious problems associated with resistance to the available acaricides, this product has been used as an important alternative to control acaricide-resistant ticks. the objective of this work was to analyse the fipronil sensitivity of ticks that were collected from farms with a history of fipronil use by larval bi ... | 2010 | 20688434 |
| differential expression of genes in salivary glands of male rhipicephalus (boophilus)microplus in response to infection with anaplasma marginale. | bovine anaplasmosis, caused by the rickettsial tick-borne pathogen anaplasma marginale (rickettsiales: anaplasmataceae), is vectored by rhipicephalus (boophilus)microplus in many tropical and subtropical regions of the world. a. marginale undergoes a complex developmental cycle in ticks which results in infection of salivary glands from where the pathogen is transmitted to cattle. in previous studies, we reported modification of gene expression in dermacentor variabilis and cultured ixodes scapu ... | 2010 | 20298599 |
| local immune response against larvae of rhipicephalus (boophilus) microplus in bos taurus indicus and bos taurus taurus cattle. | bos taurus indicus cattle are less susceptible to infestation with rhipicephalus (boophilus) microplus than bos taurus taurus cattle but the immunological basis of this difference is not understood. we compared the dynamics of leukocyte infiltrations (t cell subsets, b cells, major histocompatibility complex (mhc) class ii-expressing cells, granulocytes) in the skin near the mouthparts of larvae of r. microplus in b. t. indicus and b. t. taurus cattle. previously naïve cattle were infested with ... | 2010 | 20109460 |
| heterorhabditis amazonensis (rhabditidae: heterorhabditidae), strain rsc-5, for biological control of the cattle tick rhipicephalus (boophilus) microplus (acari: ixodidae). | the aim of this study was to evaluate the influence of different doses of heterorhabditis amazonensis rsc-5 on the biological parameters of engorged females of rhipicephalus (boophilus) microplus. the female ticks, individually identified, were divided into six groups of 20 each and exposed to the following nematode concentrations: 0, 75, 150, 300, 600, and 1,200/female. the following parameters were observed: initial weight, final weight, alteration weight, egg mass weight, pre-oviposition peri ... | 2010 | 20127363 |
| characterization of ferritin 2 for the control of tick infestations. | ixodes ricinus is one the most abundant tick species in europe and these ticks transmit pathogens causing human and animal diseases. the cattle ticks, rhipicephalus (boophilus) spp., affect cattle production in tropical and subtropical regions of the world. development of vaccines directed against tick proteins may reduce tick infestations and the transmission of tick-borne pathogens. however, a limiting step in tick vaccine development has been the identification of tick protective antigens. he ... | 2010 | 20171306 |
| evaluation of the action of heterorhabditis bacteriophora (rhabditida: heterorhabditidae) isolate hp88 on the biology of engorged females of rhipicephalus (boophilus) microplus (acari: ixodidae). | the objective of this work was to evaluate the effect of different concentrations of the entomopathogenic nematode (epn) heterorhabditis bacteriophora strain hp88 on the biological parameters of the non-parasite phase of engorged females of the cattle tick rhipicephalus (boophilus) microplus. six groups were formed, each containing 20 engorged females, which were exposed to the following concentrations of infective juveniles of this nematode: 0, 75, 150, 300, 600 and 1200 epns/female, respective ... | 2010 | 20227185 |
| silencing of three amblyomma americanum (l.) insulin-like growth factor binding protein-related proteins prevents ticks from feeding to repletion. | the insulin-like growth factor (igf) binding proteins (igfbp) family is the regulatory arm of the igf signaling system that control mitogenic and anabolic actions of igf peptide hormones. this study describes cloning and biological characterization of three amblyomma americanum (l.) (aam) proteins that show amino-terminal sequence and secondary structure similarity to the igfbp superfamily. the three molecules here provisionally identified as aamigfbp-rp1 and short (s) and long (l) aamigfbp-rp6 ... | 2010 | 20228352 |
| rotation of treatments between spinosad and amitraz for the control of rhipicephalus (boophilus) microplus populations with amitraz resistance. | a farmlet study was conducted over 4 years in which three treatments were applied to six groups of holstein dairy calves. calves in each group were infested with equal numbers of n-strain (susceptible) and ultimo strain (amitraz and synthetic pyrethroid resistant) tick larvae to establish self-sustaining populations with an initial, measurable level of resistance to amitraz. standard counts of all ticks between 4.5 and 8.0mm diameter on one side of each animal were made each week and treatment w ... | 2010 | 20079571 |
| development and validation of a pcr-rflp test to identify african rhipicephalus (boophilus) ticks. | the cattle tick rhipicephalus (boophilus) microplus has recently invaded west africa and caused anxiety amongst farmers in ivory coast, as livestock production was severely affected. the introduction of this tick species has remained unnoticed for several years, as all the members of this genus are very similar in appearance. to overcome the cumbersome morphological identification of the four closely related r. (boophilus) spp. in the region, a pcr-rflp test, based on a part of the second intern ... | 2010 | 20080073 |
| therapeutic and persistent efficacy of a long-acting (la) formulation of ivermectin against rhipicephalus (boophilus) microplus (acari: ixodidae) and sera concentration through time in treated cattle. | the concentration-time profile, therapeutic, and persistent efficacy of a single subcutaneous injection of cattle with a long-acting (la) formulation of ivermectin at a concentration of 630microg/kg of body weight were determined against rhipicephalus (boophilus) microplus. ivermectin sera concentration in treated cattle increased to 13.0ppb within 1d after treatment, and peaked at 26.2ppb at 11d post-treatment. ivermectin sera levels remained above the threshold level for control of feeding tic ... | 2010 | 20080349 |
| experimental vaccination of sheep and cattle against tick infestation using recombinant 5'-nucleotidase. | limited prior evidence suggests that 5'-nucleotidase, an ectoenzyme principally located in the malpighian tubules of the tick rhipicephalus (boophilus) microplus, could be an effective antigen in an anti-tick vaccine. to assess this, recombinant 5'-nucleotidase was expressed in escherichia coli and used in vaccination trials with both sheep and cattle. vaccinated sheep were challenged with freshly moulted adult ticks. those with high titres of anti-nucleotidase antibodies showed significant prot ... | 2010 | 20070827 |
| functional genomics tool: gene silencing in ixodes scapularis eggs and nymphs by electroporated dsrna. | ticks are blood-sucking arthropods responsible for transmitting a wide variety of disease-causing agents, and constitute important public health threats globally. ixodes scapularis is the primary vector of the lyme disease agent in the eastern and central u.s. rnai is a mechanism by which gene-specific double-stranded rna (dsrna) triggers degradation of homologous mrna transcripts. here, we describe an optimized protocol for effectively suppressing gene expression in the egg and nymphal stages o ... | 2010 | 20074328 |
| acaricidal activity and chemical composition of the essential oil from three piper species. | the chemical composition of the essential oil obtained by hydrodistillation of the aerial parts of piper amalago, piper mikanianum, and piper xylosteoides was elucidated by gas chromatography (gc) and gc/mass spectrometry analyses. p. mikanianum and p. xylosteoides essential oils presented phenylpropanoids as their main compounds (67.89% and 48.53%, respectively) whereas p. amalago was rich in monoterpene and sesquiterpene hydrocarbons (84.95%). the essential oils obtained were investigated for ... | 2010 | 20428889 |
| evidence for role of white-tailed deer (artiodactyla: cervidae) in epizootiology of cattle ticks and southern cattle ticks (acari: ixodidae) in reinfestations along the texas/mexico border in south texas: a review and update. | from 1907 when the fever tick eradication campaign began until 1933, the tick eradication methods of dipping cattle in an acaricide or "pasture vacation" were enormously successful in eradicating southern cattle ticks [rhipicephalus (boophilus) microplus (canestrini)], until failures began to occur in some areas of florida. regarding the failures in florida, the consensus was that populations of white-tailed deer [odocoileus virginianus (zimmermann)] infested with southern cattle ticks were resp ... | 2010 | 20429430 |
| genome wide scan for quantitative trait loci affecting tick resistance in cattle (bos taurus x bos indicus). | in tropical countries, losses caused by bovine tick rhipicephalus (boophilus) microplus infestation have a tremendous economic impact on cattle production systems. genetic variation between bos taurus and bos indicus to tick resistance and molecular biology tools might allow for the identification of molecular markers linked to resistance traits that could be used as an auxiliary tool in selection programs. the objective of this work was to identify qtl associated with tick resistance/susceptibi ... | 2010 | 20433753 |
| novel technique for quantifying adhesion of metarhizium anisopliae conidia to the tick cuticle. | the present study describes an accurate quantitative method for quantifying the adherence of conidia to the arthropod cuticle and the dynamics of conidial germination on the host. the method was developed using conidia of metarhizium anisopliae var. anisopliae (metschn.) sorokin (hypocreales: clavicipitaceae) and engorged rhipicephalus annulatus (say) (arachnida: ixodidae) females and was also verified for m. anisopliae var. acridum driver et milner (hypocreales: clavicipitaceae) and alphitobius ... | 2010 | 20363785 |
| [action of extract and oil neem in the control of rhipicephalus (boophilus) microplus (canestrini, 1887) (acari: ixodidae) in laboratory]. | organic plant extracts and emulsified oil of azadirachta indica a. juss (meliaceae) (neem) were studied to evaluate its effects in control of engorged females of rhipicephalus (boophilus) microplus (canestrini, 1887) in the laboratory. hexane and alcoholic organic extracts, 2% (weight/volume) were used in tests of immersion for 5 minutes, prepared with seeds, solubilized in dimethylsulfoxide (dmso) to 1%. the experiment was entirely randomized, consisting of 6 treatments and 5 replicates, each r ... | 2010 | 20385059 |
| evaluation of medicinal plant extracts against blood-sucking parasites. | the present study was based on assessments of the antiparasitic activities to determine the efficacies of acetone, chloroform, ethyl acetate, hexane, and methanol dried leaf, flower, and seed extracts of cassia auriculata l., rhinacanthus nasutus kurz., solanum torvum swartz, terminalia chebula retz., and vitex negundo linn. were tested against larvae of cattle tick rhipicephalus (boophilus) microplus canestrini, 1887 (acari: ixodidae), adult of haemaphysalis bispinosa neumann, 1897 (acarina: ix ... | 2010 | 20306205 |
| identification of a tsetse fly salivary protein with dual inhibitory action on human platelet aggregation. | tsetse flies (glossina sp.), the african trypanosome vectors, rely on anti-hemostatic compounds for efficient blood feeding. despite their medical importance, very few salivary proteins have been characterized and functionally annotated. | 2010 | 20351782 |
| ticks (acari: ixodidae) on dogs from uberlândia, minas gerais, brazil. | uberlândia in minas gerais state, southeastern brazil, has 622 000 inhabitants and is located in the cerrado biome, the south american savannah. the city dog population is estimated at 82 000 and identification of tick species and infestation prevalence on this host has not been determined. a major infectious disease of dogs in the city, canine ehrlichiosis, is transmitted by rhipicephalus sanguineus ticks. at the same time, autochthonous leishmaniosis has been recently described in the city and ... | 2010 | 20537111 |
| haemolymph protein and lipid profile of rhipicephalus (boophilus) microplus infected by fungi. | the current study evaluates the protein and lipid profile of haemolymph of rhipicephalus (boophilus) microplus engorged females infected by metarhizium anisopliae, beauveria bassiana or fusarium oxysporum. ticks were immersed or inoculated with conidial suspension. haemolymph was collected from the dorsal surface of engorged females. the results showed altered total protein amounts; however, no significant difference was observed on electrophoretic profile among haemolymph samples. in addition, ... | 2010 | 20537114 |
| humoral immune response of dairy cattle immunized with rbm95 (ku-vac1) derived from thai rhipicephalus microplus. | rhipicephalus (boophilus) microplus is an important cause of economic losses in thailand through direct effects of feeding on cattle and pathogen transmission. current tick control methods rely on expensive chemical acaricides that result in environmental contamination, residues in food animal products and acaricide-resistant ticks. anti-tick vaccines based on concealed antigens have shown promising results in the control of cattle tick. thus, recombinant bm95 (rbm95) from thai r. microplus (ku- ... | 2010 | 20537117 |
| immunization of rabbits with recombinant serine protease inhibitor reduces the performance of adult female rhipicephalus microplus. | molecules secreted from the tick salivary gland modulate the vertebrate host immune response, thus representing potential targets for novel tick control measures. tick salivary gland serine protease inhibitor (serpin) is one such molecule that may facilitate tick feeding, blood meal digestion and pathogen transmission. the objective of this study was to determine the immunogenicity and protection of recombinant rhipicephalus (boophilus) microplus salivary gland serpin (rserpin) in rabbits. rabbi ... | 2010 | 20537119 |
| reassociation kinetics-based approach for partial genome sequencing of the cattle tick, rhipicephalus (boophilus) microplus. | the size and repetitive nature of the rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. to selectively obtain gene-enriched regions of this tick's genome, cot filtration was performed, and cot-filtered dna was sequenced via 454 flx pyrosequencing. | 2010 | 20540747 |
| discovery and characterization of hemq: an essential heme biosynthetic pathway component. | here we identify a previously undescribed protein, hemq, that is required for heme synthesis in gram-positive bacteria. we have characterized hemq from bacillus subtilis and a number of actinobacteria. hemq is a multimeric heme-binding protein. spectroscopic studies indicate that this heme is high spin ferric iron and is ligated by a conserved histidine with the sixth coordination site available for binding a small molecule. the presence of hemq along with the terminal two pathway enzymes, proto ... | 2010 | 20543190 |
| localization and function of rhipicephalus (boophilus) microplus vitellin-degrading cysteine endopeptidase. | the tick rhipicephalus (boophilus) microplus is an important parasite of cattle in many areas of the tropics. characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite's physiology. the vitellin-degrading cysteine endopeptidase (vtdce) is the most active enzyme involved in vitellin hydrolysis in r. microplus eggs. here we show an association between vtdce and vitellin in an additional site, apart f ... | 2010 | 20561398 |
| haplotypes that include the integrin alpha 11 gene are associated with tick burden in cattle. | infestations on cattle by the ectoparasite boophilus (rhipicephalus) microplus (cattle tick) impact negatively on animal production systems. host resistance to tick infestation has a low to moderate heritability in the range 0.13 - 0.64 in australia. previous studies identified a qtl on bovine chromosome 10 (bta10) linked to tick burden in cattle. | 2010 | 20565915 |
| tick-susceptible bos taurus cattle display an increased cellular response at the site of larval rhipicephalus (boophilus) microplus attachment, compared with tick-resistant bos indicus cattle. | cattle demonstrate divergent and heritable phenotypes of resistance and susceptibility to infestation with the cattle tick rhipicephalus (boophilus) microplus. bos indicus cattle are generally more resistant to tick infestation than bos taurus breeds although large variations in resistance can occur within subspecies and within breed. increased tick resistance has been previously associated with an intense hypersensitivity response in b. taurus breeds; however, the mechanism by which highly resi ... | 2010 | 19852965 |
| selection of an ivermectin-resistant strain of rhipicephalus microplus (acari: ixodidae) in brazil. | resistance to ivermectin (ivm) in field populations of rhipicephalus microplus of brazil has been observed since 2001. in this work, four selection methods (infestations with: (1) ivm-treated larvae; (2) larvae from ivm-treated adult female ticks; (3) larvae from ivm-treated adult female ticks on an ivm-treated host; and (4) larvae obtained from ivm-treated females that produced eggs with a high eclosion rate) were used on a field population with an initial ivermectin (ivm) resistance ratio at l ... | 2010 | 19864067 |
| suppressive subtractive hybridization analysis of rhipicephalus (boophilus) microplus larval and adult transcript expression during attachment and feeding. | ticks, as blood-feeding ectoparasites, affect their hosts both directly and as vectors of viral, bacterial and protozoal diseases. the tick's mode of feeding means it must maintain intimate contact with the host in the face of host defensive responses for a prolonged time. the parasite-host interactions are characterized by the host response and parasite counter-response which result in a highly complex biological system that is barely understood. we conducted transcriptomic analyses utilizing s ... | 2010 | 19836138 |
| modulation of cutaneous inflammation induced by ticks in contrasting phenotypes of infestation in bovines. | tick saliva contains molecules that are inoculated at the site of attachment on their hosts in order to modulate local immune responses and facilitate a successful blood meal. bovines express heritable, contrasting phenotypes of infestations with the cattle tick, rhipicephalus (boophilus) microplus: breeds of bos taurus indicus are significantly more resistant than those of bos taurus taurus. tick saliva may contain molecules that interfere with adhesion of leukocytes to endothelium and resistan ... | 2010 | 19836891 |
| exploring the midgut proteome of partially fed female cattle tick (rhipicephalus (boophilus) microplus). | the continued development of effective anti-tick vaccines remains the most promising prospect for the control of the cattle tick, rhipicephalus (boophilus) microplus. a vaccine based on midgut proteins could interfere with successful tick feeding and additionally interfere with midgut developmental stages of babesia parasites, providing opportunities for the control of both the tick and the pathogens it transmits. midgut proteins from partially fed adult female cattle ticks were analysed using a ... | 2010 | 19840806 |
| biochemical characterization of a kunitz type inhibitor similar to dendrotoxins produced by rhipicephalus (boophilus) microplus (acari: ixodidae) hemocytes. | a novel chymotrypsin inhibitor identified in fat body and hemocyte cdna libraries of boophilus microplus was named bmci (b. microplus chymotrypsin inhibitor) (genbank eu636772). the putative bmci amino acid sequence presented a 22-residue-signal peptide and 58-residue-mature protein. bmci amino acid sequence analysis allowed its classification as a kunitz-bpti inhibitor with six cysteine residues, a theoretical pi of 7.8, and the presence of tyr at p1 position in the putative reactive site, sugg ... | 2010 | 19828254 |
| acaricidal activity of extracts from petiveria alliacea (phytolaccaceae) against the cattle tick, rhipicephalus (boophilus) microplus (acari: ixodidae). | the acaricidal activity of crude extracts and fractions from stems and leaves of petiveria alliacea (phytolaccaceae) was carried out on larvae and adults of the cattle tick rhipicephalus (boophilus) microplus using the larval immersion test (lit) and adult immersion test (ait), respectively. methanolic extracts of stems and leaves of p. alliacea showed 100% mortality on the lit bioassay. on the other hand, methanolic extracts of leaves and stem on the ait test showed 26% and 86% of mortality, re ... | 2010 | 20042296 |