Publications

[evaluation of the virulence of trichomonas vaginalis strains by the study of their cytopathogenic effect on cultured cells].	the pathogenicity level of 25 wild strains and 4 clones of trichomonas vaginalis was evaluated on the observation of rapidity and intensity of cytopathogenic effect occurring in mac coy cells culture. it appeared more sensitive, faster and nearer of behaviour and cytopathogenicity in women than subcutaneous and intraperitoneal inoculation to mice. the lack of diffusible exotoxin and the cell adhesiveness of trichomonas vaginalis observed, suggest that pathogenicity could be in connection with th ...	1982	6220793
bare trichomonas vaginalis nuclei in cervicovaginal smears.		1982	6977980
[testing the effects of hydrazones and osazones of sugars on trichomonas vaginalis (author's transl)].		1982	6978755
[study on resistant forms of trichomonas vaginalis (author's transl)].	we have demonstrated resistant forms of trichomonas vaginalis studied their morphology and induced transformation into vegetative forms. this work has led to an hypothesis concerning the parasite cycle which may throw light on the problems of transmission and resistance to therapeutic agents.	1982	6979287
comparative efficacy of go 10213 and some nitroimidazoles against trichomonas vaginalis and t. foetus in mice infected subcutaneously.		1982	6979983
polymorphonuclear cell chemotaxis to secretions of pathogenic and nonpathogenic trichomonas vaginalis.	polymorphonuclear cell chemotaxis to secretions of pathogenic and nonpathogenic strains of trichomonas vaginalis were investigated. cells from three groups, laboratory workers, patients with urogenital symptoms, and asymptomatic patients attending an antenatal clinic, all showed greater response to secretions of pathogenic strains, when the secretion was dilute. at higher concentrations of secretion, responses to both strains were similar, except in the pregnant group, where increased chemotaxis ...	1982	6980268
isoenzyme characterisation of trichomonas vaginalis.	clones of 32 strains of trichomonas vaginalis isolated from patients attending a venereal diseases clinic were compared among themselves and with authentic pentatrichomonas hominis on the basis of their isoenzyme patterns for eight enzymes by thin-layer starch-gel electrophoresis. the enzymes examined were: glucose phosphate isomerase (gpi); phosphoglucomutase (pgm); malic enzyme (nadp+) (me); hexokinase (hk); malate dehydrogenase (nad+) (mdh); glucose-6-phosphate dehydrogenase (g6pd); aldolase ...	1982	6980685
[round forms of trichomonas vaginalis and their importance in the diagnosis of trichomoniasis (author's transl)].		1982	6980798
non-gonococcal urethritis in males--a preliminary study.		1982	6980829
canine prostatic secretions kill trichomonas vaginalis.	the zinc content of prostatic secretions is thought to be an important nonspecific defense against urinary tract infection in men. this investigation measured killing by prostatic fluid of trichomonas vaginalis, a common sexually transmitted pathogen, and related this activity to zinc concentration. we used a canine model which closely resembles the human male genital tract. prostatic secretions from all dogs killed all t. vaginalis isolates. there appear to be several mechanisms for killing of ...	1982	6980837
zinc sensitivity of trichomonas vaginalis: in vitro studies and clinical implications.	the high zinc concentration (2.3-15.3 mm) in human prostatic secretions may be an important defense of the male lower urinary tract against infection by trichomonas vaginalis. trichomonads were rapidly killed by zinc salts at concentrations similar to those in prostatic fluid of normal men. there was some variation in the zinc sensitivity of 15 clinical isolates of t. vaginalis (minimal inhibitory concentration, 0.8-6.4 mm). a time-kill technique showed subtle differences in the kinetics of zinc ...	1982	6980958
[trichomonal infections in promiscuous women].	vaginal swabs taken from 2,096 promiscuous women were examined microscopically and more swabs from 1,813 women were cultivated. the women examined were divided into 4 different age groups (15-20 years, 21-25 years, 26-30 years and over 30 years). the occurrence of trichomonadosis was diminishing with the age: 65.0%--51.2%--47.7%--41.8%. the occurrence of gonorrhoea was bit lower among the youngest women (25.9%) than among the older ones, the lowest occurrence being among the group of over 30 yea ...	1982	6982010
evaluation of an enzyme-linked immunosorbent assay for the detection of antibody to trichomonas vaginalis in sera and vaginal secretions.	using a whole-cell antigen antibody to trichomonas vaginalis was measured by an enzyme-linked immunosorbent assay (elisa). igg antibody was found in sera from only three of 99 children under 12 years of age. in contrast, serum igg or igm antibody or both were detected in 80.4% of women who had vaginal trichomoniasis and in 13.7% of uninfected women. although antibody was found in cervical and vaginal secretions, the correlation between current infection and the presence of antibody was poorer th ...	1982	6982095
antibody to trichomonas vaginalis in human cervicovaginal secretions.	human cervicovaginal secretions were obtained from patients at the gynecology and obstetrics clinics at national taiwan university hospital and cathay general hospital, republic of china. among the 500 patients examined, 33 (6.6%) were infected with trichomonas vaginalis as determined by the culture method. secretions from 24 of the infected patients and 30 noninfected women were assayed for anti-t. vaginalis immunoglobulins by the indirect immunofluorescent antibody technique. a few serum sampl ...	1982	6982231
the absence of cardiolipin in hydrogenosomes of trichomonas vaginalis and tritrichomonas foetus.		1982	6982325
[laboratory diagnosis of urogenital trichomoniasis in dermatovenerologic dispensaries].		1982	6983195
screening for sexually transmissible infections in unwanted pregnancy.		1982	6983362
metronidazole resistance of trichomonas vaginalis as a cause of treatment failure in trichomoniasis--a case report.	six isolates of a strain (mrp-mt) of trichomonas vaginalis obtained from a woman before and after unsuccessful treatment with metronidazole had an appreciably lower susceptibility to metronidazole both in vitro in the aerobic tube assay and in vivo in the mouse assay than did control strains from patients cured with standard doses of the drug. our results support recent evidence that metronidazole-resistant strains of t vaginalis do cause treatment failure. resistance of these strains could be d ...	1982	6983379
sensitivity of trichomonas vaginalis to metronidazole in medium with different concentrations of iron and ascorbate.		1982	6983569
[a strain of trichomonas vaginalis with reduced sensitivity for metronidazole].		1982	6983659
trichomonas vaginalis urinary tract infection in an infant.		1982	6984181
[determination of trichomonas vaginalis sensitivity to nitroimidazole group preparations using solid nutrient media].		1982	6984259
[trichomoniasis. diagnostic problems].		1982	6984684
[vaccination with solco trichovac. immunological aspects of a new principle in the therapy and prevention of reinfection of trichomoniasis in women].		1982	6984685
[changes in the vaginal flora of trichomoniasis patients during solco trichovac vaccination].		1982	6984686
vaginitis in sexually active women: relationship to nine sexually transmitted organisms.	women seen for symptoms suggestive of vulvovaginitis were studied for the detection of mycoplasma hominis. ureaplasma urealyticum, yeast, neisseria gonorrhoeae, chlamydia trachomatis, gardnerella vaginalis, herpes simplex virus, group b beta-hemolytic streptococci, aerobes, anaerobes, and trichomonas vaginalis. asymptomatic women who reported to be sexually active and agreed to undergo comprehensive genital cultures were used as controls. there was a significant association of vulvovaginitis wit ...	1982	6280502
purine metabolism in trichomonas vaginalis.		1982	6282644
host plasma proteins on the surface of pathogenic trichomonas vaginalis.	sodium dodecyl sulfate-gel electrophoresis and fluorography and fluorography technology revealed that pathogenic trichomonas vaginalis was able to acquire numerous loosely associated plasma proteins during incubation in normal human plasma. these proteins were readily removed by repeated washing of the parasite in phosphate-buffered saline. plasma proteins avidly bound to the surface of t. vaginalis were also detected using a highly sensitive and specific agglutination assay with protein a-beari ...	1982	6288571
sexually transmitted diseases in pregnancy.	numerous sexually transmitted diseases--syphilis, gonococcal infections, group b beta-hemolytic streptococcal infection, chlamydial infections, mycoplasmas, herpes simplex virus, cytomegalovirus infections, hepatitis b viral infections, fungal infection, trichomonas vaginalis, condylomata acuminata, and scabies--are of concern when they occur during pregnancy, either because of their potential adverse effects on the fetus or the chance of a modified reaction in the pregnant woman. this article ...	1982	6293753
role of cytoskeleton in natural cell-mediated cytotoxicity against trichomonas vaginalis.		1982	6296638
the clinical diagnosis of trichomoniasis.	two hundred twenty-six consecutive women attending an inner-city clinic for sexually transmitted diseases were evaluated. problem-directed histories and physical examinations were conducted and vaginal specimens for wet preparation and trichomonas vaginalis culture were obtained from each patient. one hundred patients were found to be infected. patients with multiple sex partners were found to be at increased risk of trichomoniasis (p less than .05). those with abnormal discharge noted on examin ...	1982	6896368
[vaginal infections and the local immune response in vaginal fluid].		1982	6755245
[biocenosis of human vagina].		1982	6763577
trichomonas vaginalis: alternative pathway activation of complement.	serum from adult human donors lysed trichomonas vaginalis. the lytic effect was eliminated by heat-inactivation of serum (56 degrees c, 30 min). no serum donor exhibited significantly increased antibody against the parasite as measured by indirect immunofluorescence. treatment of serum with ethylene glycol-bis (b-aminoethyl ether)-n, n-tetraacetic acid to eliminate classical pathway complement activation did not prevent c3 leads to c3i conversion in serum incubated with t. vaginalis. release of ...	1982	6763788
human host response to giardia lamblia. i. spontaneous killing by mononuclear leukocytes in vitro.	to understand host resistance to giardia lamblia infection, we investigated the capacity of human peripheral blood effector cells to kill g. lamblia trophozoites. peripheral blood was obtained from 12 healthy, uninfected individuals without detectable igg anti-g. lamblia antibody or known exposure to g. lamblia. isolated mononuclear leukocytes (mnl), granulocytes, lymphocytes, and monocytes were cultured 16 hr with g. lamblia that had been pulsed with (3h)tdr while in log growth. mnl from all 12 ...	1982	7057036
biochemical diagnosis of vaginitis: determination of diamines in vaginal fluid.	to assess correlations between the content of the diamines putrescine and cadaverine in vaginal fluid and the clinical manifestations of vaginitis, a rapid procedure for the determination of diamines has been developed. the procedure involves thin-layer chromatography of the dansylated vaginal washings; then the fluorescence of dansyl diamines is compared with that of dansyl alanine. for 520 college women, the result of the test for diamines was positive in 173 (88%) of 197 women with nonspecifi ...	1982	7061879
single dose treatment of trichomoniasis.	oral treatment for trichomonas vaginalis infection is now well established. in a previous paper, we established the value of a high single dose regimen of metronidazole for this condition by demonstrating its efficacy in a double-blind controlled study. tinidazole is another accepted trichomonacide. by means of a single-blind controlled trial, we demonstrate that a 2 g single dose of tinidazole is as effective as a 2 g single dose metronidazole in the treatment of vaginal trichomoniasis. the cur ...	1982	7067925
vulvovaginitis in premenarcheal girls: clinical features and diagnostic evaluation.	fifty-four premenarcheal patients (median age 5.8 years) with symptoms or signs of vulvovaginitis were studied, and the results of cultures of vaginal secretions were compared with those from an age-matched control group. vaginal discharge was found on examination in 24 of 42 patients with a complaint of discharge, and in two of 12 patients without a complaint of discharge. convincing evidence of bacterial or monilial infection was found in 14 of the 26 patients with discharge on examination, bu ...	1982	7099784
urogenital involvements and musculoskeletal complaints in women. an interview study and clinical investigation with special reference to chlamydial infections.	to study the correlation of urogenital involvements and musculoskeletal complaints in females, 311 randomly selected women, aged 15 to 54, were interviewed. in addition, clinical gynecological examination including isolation and serology of chlamydia trachomatis (ct) was performed for 30 consecutive volunteers. a history of musculoskeletal complaints occurred in 218 (70.1%) and a history of urogenital involvements in 244 (78.5%) of the 311 women. the number of urogenital involvements in 218 fema ...	1982	7123176
chlamydia trachomatis infections in women with urogenital symptoms.	chlamydia trachomatis was isolated from 30 to 100 women attending a family physician's office with dysuria, frequency or vaginal discharge, compared with 2 of 30 asymptomatic women. multiple infections were common: c. trachomatis coexisted with gardnerella vaginalis, candida albicans, trichomonas vaginalis or a bacterial cause of urinary tract infection in 15 patients. c. trachomatis was isolated alone from 15 symptomatic women. the source of the positive culture was not always the site of sympt ...	1982	7139448
pharmacokinetics of tinidazole in male and female subjects.	tinidazole is a potent nitroimidazole compound active against, and used to treat, trichomonas vaginalis infections in males and females. speculation exists in the literature that observed differences in tinidazole plasma concentrations between males and females may be due to sex-mediated pharmacokinetic differences. to investigate this phenomenon, a study was designed to determine the pharmacokinetics of tinidazole in male and female subjects. six male and six female volunteers were each adminis ...	1982	7161410
clinic diagnosis of anaerobic vaginosis (non-specific vaginitis). a practical guide.	anaerobic vaginosis (non-specific vaginitis) can be readily diagnosed at the time of first attendance without recourse to expensive and time-consuming laboratory investigations. diagnosis is based on careful history-taking, clinical examination, and simple investigations in the clinic. although the presence of a malodorous vaginal discharge and the finding of clue cells on microscopy (in the absence of trichomonas vaginalis) are sufficient for the diagnosis, a positive amine test and a vaginal p ...	1982	7171981
intrauterine devices (iuds) and their complications (clinical evaluation of 2013 women with iud in-situ).	iud related events were evaluated at the department of obstetrics and gynecology, hacettepe university in ankara, turkey. the study population included 2213 fertile, healthy women who had an iud in situ. the age range was 17-48 years. all iuds were lippes loop, size c and d, and they were inserted by an experienced physician and nurse on the 5th day of menstruation. the follow-up time was 1 year in 2013 women with iuds. there was no follow-up on 200 women who were excluded from the study. ...	1982	12267258
association of trichomonas vaginalis with other microorganisms.		1982	6122867
gardnerella vaginalis, anaerobes, and vaginal discharge.		1982	6123676
metronidazole-resistant trichomonas vaginalis.		1982	6119430
[detection of trichomonas vaginalis by fluorescent staining technique (author's transl)].		1982	6179722
a rapid method for detection of trichomonas vaginalis.		1982	6181370
studies of cervicovaginal smears for the presence of actinomycetes.	cytologic and direct fluorescent antibody techniques were used to detect the presence of actinomyces israelii and arachnia propionica in cervicovaginal smears collected from 100 women, 94 of whom did not use intrauterine contraceptive devices. in no case were these organisms found. the possible significance of these results is discussed.	1983	6195527
trichomonal antibody in male homosexuals.		1983	6132213
[oral contraceptive use and prevalence of infection with chlamydia trachomatis in women (author's transl)].	1080 nonpregnant women ages 16-34 years, presenting for the 1st time at a clinic for sexually transmitted diseases (std), were examined and screened for infection with chlamydia trachomatis, neisseria gonorrheae, trichomonas vaginalis, and candida species. the respective prevalence rates were 21.1%, 20.7%, 13.4%, and 27.8%. isolation rates for chlamydia trachomatis, either occurring alone or in association with other genital infections, were significantly greater in women using oral contracept ...	1983	12338679
the vaginal flora of women in zimbabwe.	high vaginal and cervical swabs were taken from 199 pregnant women and 156 nonpregnant patients attending an std clinic in zimbabwe. trichomonas vaginalis was isolated from 31% of pregnant women and 37% of std patients. the bacterial flora in both groups was similar to that found in women from developed countries except that fecal organisms were more and lactobacilli less commonly isolated. n. gonorrheae was not isolated from any of the pregnant women, but was isolated from 13 of the std pati ...	1983	12340187
epidemiology and clinical diagnosis of trichomonas vaginalis infection in zimbabwe.		1983	6616578
serological study with solcotrichovac, a vaccine against trichomonas vaginalis infection in women.		1983	6629134
nonspecific vaginitis and other genital infections in three clinic populations.	nonspecific vaginitis was present in 37% of 40 patients attending the special urology (sexually transmitted disease) clinic at the victoria general hospital, in 23% of 75 patients attending the family planning clinic, and in 23% of 13 patients attending the prenatal clinic at the grace maternity hospital in halifax, nova scotia. the mean prevalence was 27%. subjective complaints of vaginal odor were significantly associated with nonspecific vaginitis (p less than .001), but symptoms of vaginal d ...	1983	6648745
induction of lung tumors and lymphomas in balb/c mice by metronidazole.	metronidazole, which is widely used in the treatment of "trichomonas vaginalis", "entamoeba histolytica" and "giardia lamblia" infections, was administered to balb/c mice by stomach tube in an aqueous solution at a dose rate of 2 mg/day for 100 days (total 200 mg) to test its carcinogenicity. the treatment induced a significant increase in lung tumors in male mice (p less than 0.001) and provoked the appearance of lymphomas in females (p less than 0.001). although there is insufficient evidence ...	1983	6649066
[statistical evaluation and diagnostic consideration on trichomoniasis and candidiasis in vaginal pathology].	from 1980 to 1982, we studied 1,827 cases of vaginal smears with particular interest in positive cases of trichomonas vaginalis and candida sp. moreover, we studied the statistical incidence of these microorganisms, and also some diagnostic problems, and the effects of these agents on vaginal microflora.	1983	6675046
vaginal infections. how to identify and treat them.	vaginal infection occurs at some time in the majority of women. most such infections are caused by candida albicans, trichomonas vaginalis, and gardnerella (hemophilus) vaginalis. some less common--but in many cases increasingly prevalent--causes include herpes-virus type 2, papillomavirus, chlamydia trachomatis, mycoplasma t strains, and toxic shock syndrome.	1983	6681672
urine as a specimen for diagnosis of sexually transmitted diseases.	the case of specimen collection has led to the suggestion that urine might be a useful specimen for the isolation of sexually transmitted disease agents. it would only be an appropriate specimen for agents that infect the urethra, such as neisseria gonorrhoeae or chlamydia trachomatis. comparative tests have shown that culture of urine for chlamydiae (from men with urethritis) or for gonococci from women is an insensitive procedure. gonococci can be isolated from urine from men at rates essentia ...	1983	6309002
human milk kills parasitic intestinal protozoa.	giardia lamblia, a common pathogenic intestinal parasite of humans, was rapidly killed by exposure to normal human milk in vitro. the killing did not depend on secretory immunoglobulin a. entamoeba histolytica, the dysentery amoeba, was also killed by normal human milk. giardia-cidal activity cochromatographed with an unusual lipase that is present in the milk of humans but not of lower mammals. human milk may play a protective role in infants exposed to this parasite.	1983	6310751
the pathway of arginine catabolism in the parasitic flagellate trichomonas vaginalis.	arginine is rapidly depleted from the medium during the cultivation of t. vaginalis in a defined or semi-defined medium. it is broken down to ornithine, ammonia and carbon dioxide by the three enzymes of the dihydrolase pathway: arginine deiminase, catabolic ornithine carbamyltransferase (octase) and carbamate kinase. arginase and urease as well as citrulline hydrolase appear to be absent. ornithine, a product of the pathway was further converted to putrescine by an active ornithine decarboxylas ...	1983	6312311
hydrogenosomal ferredoxin of the anaerobic protozoon, tritrichomonas foetus.	a low molecular weight iron-sulfur protein has been purified from tritrichomonas foetus by deoxycholate extraction of whole cells, ion exchange chromatography, and gel filtration. the purified protein was essentially homogeneous as judged by isoelectric focusing, polyacrylamide gel electrophoresis, and gel filtration. a pi of 4.3 was observed. the molecular weight of the protein was estimated to be 12,000. chemical and spectral analysis showed the protein to have a [2fe-2s] cluster. the absorban ...	1983	6313659
[cultivation of t. vaginalis using a new medium].		1983	6334886
identification of immunogenic and antibody-binding membrane proteins of pathogenic trichomonas vaginalis.	characterization of immunogenic trichomonas vaginalis membrane proteins was accomplished by using extrinsically and intrinsically labeled organisms and a highly sensitive and specific radioimmunoprecipitation procedure. intact motile trichomonads were compared with detergent extracts as a source of antigen in radioimmunoprecipitation experiments. approximately 20 proteins accessible to antibody were identified and ranged in molecular weight from 200,000 to 20,000. localization on the parasite su ...	1983	6339404
an analysis of the proteinases of trichomonas vaginalis by polyacrylamide gel electrophoresis.	the proteinases of trichomonas vaginalis have been analysed by electrophoresis in polyacrylamide gels containing denatured haemoglobin. seven bands of activity were detected indicating multiple proteinases. all of the enzymes were stimulated by 1 mm dithiothreitol and had inhibitor sensitivities characteristic of cysteine proteinases. the enzymes differed significantly, however, with respect to ph optima and relative sensitivities to inhibitors.	1983	6340036
antigenic analysis of trichomonas vaginalis strains by quantitative fluorescent antibody methods.	clones of 5 trichomonas vaginalis strains, jh30a, jh31a, jh34a, jh162a, and jh384a, kept in liquid nitrogen (with dmso serving as the cryoprotectant) after a few weeks of cultivation in axenic cultures, were analyzed for their antigenic properties by quantitative direct fluorescent antibody methods. measurements of fluorescence of individual cells obtained with the aid of an ultramicrofluorometer indicated that each strain possessed unique antigens as well as antigens it shared with the other st ...	1983	6344477
[relation between chlamydia trachomatis and trichomonas vaginalis in vitro].		1983	6344497
susceptibility of trichomonas vaginalis strains to metronidazole: response to treatment.	the effect of the susceptibility of trichomonas vaginalis strains to metronidazole on response to treatment was determined from minimal inhibitory concentrations (mics) for organisms isolated during a clinical trial in which single 1- and 2-g doses of metronidazole were compared. fifty-seven strains were isolated from patients receiving 1 g of metronidazole, and 75 from those receiving 2 g. the mean mic for all strains was 1.50 microgram/ml (range, 0.5-4.5 microgram/ml) and was similar in both g ...	1983	6359490
[disorders of vaginal biocenosis in women with risk of genital cancer].	473 women 38-71 years old were examined. 55 women with physiologic biocenosis of the vagina were included into control group. in remaining (418) there were detected: erosions, cancers of the cervix, hyperplasias and cancers of the endometrium of the uterine body, inflammations, leukoplakia and cancers of the vulva. microbiological examinations consisted if indirect examinations of vaginal content and of artificial cultures on different media. disturbances in vaginal biocenosis was diagnosed in t ...	1983	6371790
phagocytosis and killing of neisseria gonorrhoeae by trichomonas vaginalis.	type 2 and 4 transparent and opaque neisseria gonorrhoeae demonstrated a logarithmic loss of viability with a half life of approximately 10-30 min when incubated in the presence of trichomonas vaginalis. although this effect was observed in the absence of serum for most types of gonococci tested, it was consistently enhanced by the addition of human serum. only for type 4 transparent gonococci did this process show an absolute serum requirement. cytochalasin b inhibited the loss of viability. th ...	1983	6401789
[bacteriologic study of urethritis].		1983	6404989
microbiology of vaginal discharge in nairobi, kenya.	among women attending a sexually transmitted disease (std) clinic in nairobi with vaginal discharge, neisseria gonorrhoeae and chlamydia trachomatis were isolated from the cervix in 32 (26%) of 122 and four (7%) of 58 women respectively. infection with trichomonas vaginalis, candida albicans, gardnerella vaginalis, and mycoplasma spp were diagnosed in 42 of 122 (34%), 26 of 110 (24%), 75 of 100 (75%), and 42 of 89 (47%) women respectively. mixed infections with at least two pathogens were found ...	1983	6405973
evaluation of two serological tests for trichomonas vaginalis infection.	trichomonas vaginalis is a widely prevalent, sexually transmitted protozoan infecting both males and females. despite its prevalence, little is known about its contribution to the morbidity rates for urogenital-tract infections. currently accepted diagnostic methods are limited to the demonstration of the organism in fresh material, identification in stained material, or in vitro cultivation of organisms from the urogenital tract. we have evaluated the indirect hemagglutination test and the gel ...	1983	6408119
infection of female squirrel monkeys (saimiri sciureus) with trichomonas vaginalis as a model of trichomoniasis in women.	marmosets, tamarins, owl monkeys, and squirrel monkeys were inoculated intravaginally with trichomonas vaginalis. the latter animal species was most susceptible, trichomonads being recovered from four of six monkeys for periods ranging usually up to one month. vaginal discharge was seen in most infected animals but polymorphonuclear leucocytes were rare. infected animals developed serum igg antibody responses and also local igg and iga responses, the latter occurring sometimes on reinoculation i ...	1983	6409346
[the effect of spermicides on physiological and pathogenic genital flora].	in vitro-studies concerning the influence of commercially available spermicides on lactobacillus acidophilus as well as on pathogenic organisms of the genital tract revealed, that the preparations had only weak antimicrobial effect on döderlein's bacteria and pathogenic fungi. however, all four preparations tested, revealed a good inhibition effect on neisseria gonorrhoeae, treponema pallidum and trichomonas vaginalis. irreversible damage to gonococci and trichomonas vaginalis. irreversible dama ...	1983	6410591
the activity of fexinidazole (hoe 239) against experimental infections with trypanosoma cruzi, trichomonads and entamoeba histolytica.	a new 5-nitroimidazole compound, 1-methyl-2-(4-methylthiophenoxymethyl)-5-nitroimidazole: fexinidazole, hoe 239, was found to be highly active against experimental infections with trypanosoma cruzi, tritrichomonas foetus, trichomonas vaginalis and entamoeba histolytica. it was slightly superior to benznidazole and nifurtimox in suppressing parasitaemia in mice infected with t. cruzi. it also eradicated the parasites from infected mice after a prolonged treatment period. the order of activity of ...	1983	6411009
[chlamydia trachomatis in the urogenital tract of pregnant women].	in two hundred and thirty three prenatal patients from the prenatal clinic of the university of würzburg cervical, vaginal and urethral swabs were taken for microscopic examination and culture for chlamydia trachomatis (c. trachomatis), neisseria gonorrhoeae (n. gonorrhoeae), trichomonas vaginalis (t. vaginalis), beta-hemolytic streptococci, candida species, ureaplasma urealyticum (u. urealyticum), mycoplasma species and facultatively pathogenic and apathogenic aerobic bacteria. in ten cases (4% ...	1983	6411515
micro-counterimmunoelectrophoresis: a rapid screening technique for trypanosomiasis.	a rapid and economical micro-counterimmunoelectrophoresis (mcie) test, for antibody to trypanosome antigens, has been developed which may prove useful in trypanosomiasis surveillance. mcie was more sensitive and more rapid than immunodiffusion (id). serum samples from trypanosome-infected rabbits and cattle (nigerian and gambian), from kuwaiti dogs and camels, from ivory coast sleeping sickness patients, from brazilian patients with chagas's disease, plus mouse-anti-malaria sera have been tested ...	1983	6415874
abc of sexually transmitted diseases. urethral discharge: diagnosis.		1983	6416414
failure to detect extranuclear dna in trichomonas vaginalis and tritrichomonas foetus.		1983	6600788
gardnerella vaginalis and anaerobic bacteria in genital disease.	in a study of gardnerella vaginalis and anaerobic bacteria in non-specific vaginitis (nsv) and other genital disease 89 patients attending a genital medicine clinic had vaginal samples examined for conventional pathogens and for quantitative analysis of g vaginalis and aerobic and anaerobic bacterial flora. the overall incidence of g vaginalis was 20%; g vaginalis (mean concentration 7.0 log10/g of secretion) occurred predominantly in patients with nsv (57%) but also in sexual contacts of non-sp ...	1983	6600955
antitrichomonal activity of compounds that affect dna and its repair.	the trichomonacidal activity of dimethyl sulphate (dms) was increased a 100-fold by administration in combination with benzamide and aminobenzamides, compounds thought to interfere with dna repair in mammalian cells. the same benzamides did not enhance the trichomonacidal activities of metronidazole or a thiosemicarbazone. the results suggest that trichomonas vaginalis is able to repair damaged dna and that neither metronidazole nor the thiosemicarbazone affect trichomonal dna in the same way as ...	1983	6601103
antigen-specific proliferation responses of peripheral blood lymphocytes to trichomonas vaginalis antigen in patients with trichomonas vaginitis.	this report describes the development of an assay system which overcomes the difficulty of detecting immune responses of patients with trichomonas vaginitis by making use of peripheral blood leukocytes obtained from such patients. when peripheral blood leukocytes from the patient were stimulated in microcultures with the soluble antigen extracted from trichomonas vaginalis, significant degrees of proliferation ensued, as measured by the incorporation of [methyl-3h]thymidine 4 to 5 days after ini ...	1983	6601112
trichomonas vaginalis in men.		1983	6601192
association of mast cells with vaginal trichomoniasis in endocervical smears.	in endocervical smears obtained from 40 women with trichomonas vaginalis infection, 25 women with infections other than t. vaginalis and 35 cytologically normal women, inflammatory cells consistent with mast cells (tissue basophils) were examined. mast cells were present predominantly in the smears of patients infected with t. vaginalis but were infrequent in those of patients with other infections and in those of the normal, control group. the numbers of mast cells in endocervical smears and th ...	1983	6601348
antigen analysis of several pathogenic strains of trichomonas vaginalis.	analysis of several human strains of trichomonas vaginalis and one bovine strain of tritrichomonas foetus was accomplished with standard sodium dodecyl sulfate-gel electrophoresis and fluorography technology. highly motile, live trichomonads were radiolabeled, and total trichloroacetic acid-precipitated proteins were electrophoresed. complex protein profiles of the various human strains of t. vaginalis were obtained with proteins ranging in molecular weight from 20,000 to greater than 200,000. t ...	1983	6601618
acquisition of alpha 1-antitrypsin by a pathogenic strain of trichomonas vaginalis.	the interaction of alpha 1-antitrypsin, the major serine protease inhibitor in plasma, with pathogenic trichomonas vaginalis and the acquisition by trichomonads of this host protein from normal human plasma were investigated. alpha 1-antitrypsin acquired by intact parasites could not be removed by repeated washings in phosphate-buffered saline. saturation kinetics were observed after incubation of glutaraldehyde-fixed organisms with 125i-labeled alpha 1-antitrypsin. evidence suggesting that spec ...	1983	6601623
purine and pyrimidine metabolizing activities in trichomonas vaginalis extracts.		1983	6601772
isolation of trichomonas vaginalis from the respiratory tract of infants with respiratory disease.	trichomonas vaginalis was isolated from the respiratory tracts of two infants with respiratory disease. bacterial and viral cultures of tracheal aspirates and nasopharyngeal specimens did not detect bacterial pathogens or viruses; however, t vaginalis was found to be present in inoculated cell cultures. both infants were delivered vaginally by mothers with known previous episodes of t vaginalis infection. the possibility that this organism may, on occasion, cause respiratory tract disease needs ...	1983	6602324
analysis of surface saccharides in trichomonas vaginalis strains with various pathogenicity levels by fluorescein-conjugated plant lectins.	certain surface saccharides of organisms from clone-derived cultures of five trichomonas vaginalis strains, jh30a-cl. 1, jh31a-cl. 1, jh32a-cl. 1, jh34a-cl. 1, jh162a-cl. 1, and jh384a-cl. 2, which differed in their pathogenicity for women and experimental hosts, were compared with the aid of fluorescein-conjugated plant lectins using a quantitative fluorescence method. the lectins used were: concanavalin a (con-a), wheat germ agglutinin (wga), soybean agglutinin (sba), castor bean agglutinin (c ...	1983	6602431
epidemiology and clinical diagnosis of trichomonas vaginalis infection in zimbabwe.		1983	6603271
immune response to trichomonas vaginalis. iv. immunochemical and immunobiological analyses of t. vaginalis antigen.	the immune response of patients with trichomoniasis vaginitis to trichomonas vaginalis was examined by using passive hemagglutination assay and skin reactions. trichomonas-specific immune responses could hardly be detected in the patients by these assay methods. in order to develop a simple and reliable assay for demonstration of t. vaginalis-specific immune responses in the patients, proliferation responses of peripheral mononuclear cells from the patients were examined. lymphocytes obtained fr ...	1983	6603425
the antagonistic effects of acetate and lactate upon the trichomonacidal activity of metronidazole.	lactate and acetate at 5 mm were found to be antagonistic to the killing action of metronidazole on trichomonas vaginalis under aerobic but not anaerobic conditions. these compounds had no effect on the growth of the parasite, or the rate of metronidazole reduction by, and the activities of nadh oxidase and nadph oxidase in, parasite homogenates. the phenomenon reported could be one reason why metronidazole is not always effective in the treatment of trichomoniasis.	1983	6603456
natural macrophage cytotoxicity against trichomonas vaginalis is mediated by soluble lytic factors.	mechanisms of lysis of the extracellular protozoan trichomonas vaginalis by uninduced resident macrophages were analyzed. supernatants obtained by culturing such macrophages with t. vaginalis were cytotoxic for the protozoa in a dose-dependent manner. supernatants from macrophages cultured alone were cytotoxic at lower levels, whereas those obtained from t. vaginalis alone and from macrophages cultured with unrelated cells (b77) were not cytotoxic. cytotoxic activity appeared after 4 h of contac ...	1983	6604025
beta-hemolytic activity of trichomonas vaginalis correlates with virulence.	the reasons that some women develop symptomatic trichomonal vaginitis, whereas many other infected women remain asymptomatic, are unclear, but it has been suggested that trichomonas vaginalis strains vary in their intrinsic virulence. we describe beta-hemolytic activity in t. vaginalis which correlates with virulence in patients as well as in an animal model and in tissue culture. fresh t. vaginalis isolates from four women with severe, symptomatic trichomoniasis had high-level (86.3 +/- 6.6%) h ...	1983	6604026
amine content of vaginal fluid from patients with trichomoniasis and gardnerella associated non-specific vaginitis.	amounts of putrescine, cadaverine, and tyramine were measured in vaginal washings from five patients with non-specific vaginitis (nsv) associated with gardnerella vaginalis, five patients with trichomoniasis, and five healthy controls. putrescine and cadaverine were present in all but one sample from the infected patients; tyramine was found in four of five samples from the women with nsv and in two of five samples from those with trichomoniasis. the mean cadaverine to putrescine ratio was signi ...	1983	6604557
[diagnostic and epidemiologic significance of multinuclear and round forms of trichomonas vaginalis].	trichomonas are analyzed in the material taken directly from the urogenital tract of male and female patients cultivated on roiron medium and using different methods for staining the preparations was carried out. it is observed that some of the preparations examined show round nuclear forms of t. vaginalis impossible the detect by laboratory methods used up to now. the techniques used in this study decrease the risk of false diagnosis of these forms of trichomoniasis by less experienced examiner ...	1983	6605001
unusual and giant forms of trichomonas vaginalis.		1983	6605014
viability of trichomonas vaginalis in vitro at four temperatures.	the effect of temperature as a possible factor on the survival of trichomonas vaginalis for shipment or routine laboratory maintenance was studied. ten strains of t. vaginalis, atcc 30001, atcc 30238, and eight clinical isolates, were examined for viability when kept incubated at 37 degrees c or removed from this temperature and held at 42, 22, or 5 degrees c for increasing lengths of time without subculture or reincubation at 37 degrees c. the order in which the strains remained viable without ...	1983	6605364
purine salvage networks in giardia lamblia.	purine metabolism in giardia lamblia was investigated by monitoring incorporation of radiolabeled precursors into purine nucleotides in the log-phase trophozoites cultivated in vitro in axenic media and incubated in buffered saline glucose. the lack of incorporation of formate, glycine, hypoxanthine, inosine, and xanthine into the nucleotide pool suggests the absence of de novo purine nucleotide synthesis and the inability to form imp as the precursor of amp and gmp in g. lamblia. only adenine, ...	1983	6605408