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integrated versus stand-alone second generation ethanol production from sugarcane bagasse and trash.ethanol production from lignocellulosic materials is often conceived considering independent, stand-alone production plants; in the brazilian scenario, where part of the potential feedstock (sugarcane bagasse) for second generation ethanol production is already available at conventional first generation production plants, an integrated first and second generation production process seems to be the most obvious option. in this study stand-alone second generation ethanol production from surplus su ...201222019267
sugarcane improvement: how far can we go?in recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. it has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ests had limited impact for breeding. until recently, the models used by breeders in statistical genetics approaches have been developed for diploid organisms, which are not ideal for a polyploid genome such as that of sugarcane. breeding programs are de ...201221983270
diversification of hat transposase paralogues in the sugarcane genome.transposons are abundant components of eukaryotic genomes, and play important role in genome evolution. the knowledge about these elements should contribute to the understanding of their impact on the host genomes. the hat transposon superfamily is one of the best characterized superfamilies in diverse organisms, nevertheless, a detailed study of these elements was never carried in sugarcane. to address this question we analyzed 32 cdnas similar to that of hat superfamily of transposons previous ...201222228195
a mixed model qtl analysis for sugarcane multiple-harvest-location trial data.sugarcane-breeding programs take at least 12 years to develop new commercial cultivars. molecular markers offer a possibility to study the genetic architecture of quantitative traits in sugarcane, and they may be used in marker-assisted selection to speed up artificial selection. although the performance of sugarcane progenies in breeding programs are commonly evaluated across a range of locations and harvest years, many of the qtl detection methods ignore two- and three-way interactions between ...201222159754
acetobacter okinawensis sp. nov., acetobacter papayae sp. nov., and acetobacter persicus sp. nov.; novel acetic acid bacteria isolated from stems of sugarcane, fruits, and a flower in japan.eleven strains of acetic acid bacteria were isolated from stems of sugarcane, fruits, and a flower in japan. the isolates were separated into three groups, groups i, ii, and iii, in the genus acetobacter according to phylogenetic analysis based on 16s rrna sequences. the isolates had sequence similarities of 99.8-100% within the group, 99.3-99.6% to those of the type strains of each related acetobacter species, and less than 98.4% to those of the type strains of other acetobacter species. genomi ...201222878741
functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides.plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. cost effective production of enzymes and identification of efficient degradation routes are two economic bottlenecks that currently limit the use of renewable feedstocks through an environmental friendly pathway. the present study describes the hypersecretion of an endo-xylanase (gh11) and an arabinofuranosidase (gh54) by a fungal expression system w ...201222750495
recombinant expression and biochemical characterization of sugarcane legumain.plant legumains, also termed vacuolar processing enzymes (vpes), are cysteine peptidases that play key roles in plant development, senescence, programmed cell death and defense against pathogens. despite the increasing number of reports on plant cysteine peptidases, including vpes, the characterization of sugarcane vpes and their inhibition by endogenous cystatins have not yet been described. this is the first report of the biochemical characterization of a sugarcane cysteine peptidase. in this ...201222721948
a novel multienzyme complex from a newly isolated facultative anaerobic bacterium, paenibacillus sp. tw1.a multienzyme complex from newly isolated paenibacillus sp. tw1 was purified from pellet-bound enzyme preparations by elution with 0.25% sucrose and 1.0% triethylamine (tea), ultrafiltration and sephacryl s-400 gel filtration chromatography. the purified multienzyme complex showed a single protein band on non-denaturing polyacrylamide gel electrophoresis (native-page). the high molecular mass of the purified multienzyme complex was approximately 1,950 kda. the complex consisted of xylanase and c ...201222695526
identification of sense and antisense transcripts regulated by drought in sugarcane.sugarcane is an important sugar and energy crop that can be used efficiently for biofuels production. the development of sugarcane cultivars tolerant to drought could allow for the expansion of plantations to sub-prime regions. knowledge on the mechanisms underlying drought responses and its relationship with carbon partition would greatly help to define routes to increase yield. in this work we studied sugarcane responses to drought using a custom designed oligonucleotide array with 21,901 diff ...201222610347
influence of sugarcane burning on indoor/outdoor pah air pollution in brazil.this work presents the influence of sugarcane burning on pah levels and their profiles at a residence located in araraquara (sp, brazil), a city surrounded by sugarcane plantations. the average concentrations of total pahs (σpahs) associated with atmospheric particulate matter were higher during the burning period (σpahs 22.9 ng m(-3)) than in the non-burning period (σpah 2.35 ng m(-3)). a comparison of our results with previous studies regarding pah levels and their profiles in araraquara outdo ...201222578797
establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes.a good culture system provides considerable quantities of highly regenerable target tissues. embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. effective exploitation of genetic transformation requires good regeneration systems. we selected three sugarcane genotypes for the establishment and optimization of good in vitro regeneration systems, viz., s-2003-us-359, s-2006-sp-30, and s-2003-us-165. three callus inductio ...201222535387
a bac library of the sp80-3280 sugarcane variety (saccharum sp.) and its inferred microsynteny with the sorghum genome.sugarcane breeding has significantly progressed in the last 30 years, but achieving additional yield gains has been difficult because of the constraints imposed by the complex ploidy of this crop. sugarcane cultivars are interspecific hybrids between saccharum officinarum and saccharum spontaneum. s. officinarum is an octoploid with 2n = 80 chromosomes while s. spontaneum has 2n = 40 to 128 chromosomes and ploidy varying from 5 to 16. the hybrid genome is composed of 70-80% s. officinaram and 5- ...201222524198
mass balance of pilot-scale pretreatment of sugarcane bagasse by steam explosion followed by alkaline delignification.five pilot-scale steam explosion pretreatments of sugarcane bagasse followed by alkaline delignification were explored. the solubilised lignin was precipitated with 98% sulphuric acid. most of the pentosan (82.6%), and the acetyl group fractions were solubilised during pretreatment, while 90.2% of cellulose and 87.0% lignin were recovered in the solid fraction. approximately 91% of the lignin and 72.5% of the pentosans contained in the steam-exploded solids were solubilised by delignification, r ...201222391588
production of a his-tagged canecystatin in transgenic sugarcane.transgenic plants have been widely used as expression systems of recombinant proteins in recent years because it can be an efficient alternative for the large-scale production of proteins. this is an area with great potential but is still not much explored. indeed, this system can bring a breakthrough in the expression of any protein. the model used here as a protein factory was sugarcane, a crop of great global importance. this chapter describes the system that has been adopted in the routine p ...201222351027
study of postures in sugarcane cutters in the pontal of paranapanema-sp, brazil.the expansion of sugarcane monoculture in brazil in the last decades has pointed out to the necessity of considering the question of sugarcane cutters occupational health. in this work we present a cross-sectional study aiming to examine the occupational posture of a group of sugarcane cutters, which work in a cane field located in the region of pontal do paranapanema- sp, brazil. the study was made using the ergonomic analysis of work - eaw methodology and the postural analysis method by win-ow ...201222317560
impacts of introducing a new tool for the manual harvesting of sugar cane: the ergonomic analysis contribution.the purpose of this study was to evaluate the impact of introduction a new work tool on health and productivity of manual harvesting sugarcane workers. in this study it was performed aergonomic work analysis (ewa) to analyze the work tool changes.the study included sugarcane workers, drivers, fiscals, and technical's safety at work.during the analysis process, 134 questionnaires were applied,aimed to identify the constraints that such workers are submitted,to evaluate the tool introduced, in ord ...201222317488
protective gloves on manual sugar cane cutting are really effective?problems related to the use of personal protective equipment (ppe), specially the use of protective gloves for the manual sugar cane cutting, motivated this research, made possible by a tripartite negotiation involving the ministry of labor, the union of rural workers and the employer's association of sugarcane agribusiness. the main objective was to evaluate, from an ergonomics perspective, the impact of use of the gloves during the manual cane sugar cutting, raising questions on safety, effect ...201222317487
biocatalytic potential of an alkalophilic and thermophilic dextranase as a remedial measure for dextran removal during sugar manufacture.the present study is focused on dextranase from streptomyces sp. nk458 with potential to remove dextran formed during sugar manufacture. the dextranase had molecular weight of 130 kda and hydrolyzed 15-25 and 410 kda dextran. dextranase production was optimized using statistical designs and the enzyme was purified 1.8-fold with 55.5% recovery. it displayed maximum activity at ph 9.0 and 60°c and was stable over a wide range of ph from 5.0 to 10.0. the k(m) and v(max) values were 3.05 mm and 17.9 ...201222277209
effects of soil mechanical resistance on nematode community structure under conventional sugarcane and remaining of atlantic forest.nematodes present high potential as a biological indicator of soil quality. in this work, it was evaluated relations between soil physical properties and nematode community under sugarcane cropping and remaining of atlantic forest areas in northeastern pernambuco, brazil. soil samples were collected from september to november 2009 along two 200-m transects in both remaining of atlantic forest and sugarcane field at deeps of 0-10, 10-20, 20-30, 30-40, and 40-50 cm. for soil characterization, it w ...201221769561
bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation.depleted supplies of fossil fuel, regular price hikes of gasoline, and environmental damage have necessitated the search for economic and eco-benign alternative of gasoline. ethanol is produced from food/feed-based substrates (grains, sugars, and molasses), and its application as an energy source does not seem fit for long term due to the increasing fuel, food, feed, and other needs. these concerns have enforced to explore the alternative means of cost competitive and sustainable supply of biofu ...201223251086
correlation between agar plate screening and solid-state fermentation for the prediction of cellulase production by trichoderma strains.the viability of converting biomass into biofuels and chemicals still requires further development towards the reduction of the enzyme production costs. thus, there is a growing demand for the development of efficient procedures for selection of cellulase-producing microorganisms. this work correlates qualitative screening using agar plate assays with quantitative measurements of cellulase production during cultivation under solid-state fermentation (ssf). the initial screening step consisted of ...201223227312
genome sequence of enterobacter sp. strain sp1, an endophytic nitrogen-fixing bacterium isolated from sugarcane.enterobacter sp. strain sp1 is an endophytic nitrogen-fixing bacterium isolated from a sugarcane stem and can promote plant growth. the draft genome sequence of strain sp1 presented here will promote comparative genomic studies to determine the genetic background of interactions between endophytic enterobacteria and plants.201223209221
functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum.transposable elements (tes) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%-80% of the genomic content. tes have recently been shown to be a source of new genes and new regulatory networks. the most striking contribution of tes is referred as "molecular domestication", by which the element coding sequence loses its movement capacity and acquires cellular function. recently, domesticated transposases known as mustang and derived from the muta ...201223055803
whole-genome sequence of enterobacter sp. strain sst3, an endophyte isolated from jamaican sugarcane (saccharum sp.) stalk tissue.enterobacter sp. strain sst3 is an endophytic bacterium isolated from saccharum spp. here we present its annotated draft genome that may shed light on its role as a bacterial endophyte of sugarcane. to our knowledge, this is the first genome announcement of a sugarcane-associated bacterium from the genus enterobacter.201223045495
a review on soil carbon accumulation due to the management change of major brazilian agricultural activities.agricultural areas deal with enormous co2 intake fluxes offering an opportunity for greenhouse effect mitigation. in this work we studied the potential of soil carbon sequestration due to the management conversion in major agricultural activities in brazil. data from several studies indicate that in soybean/maize, and related rotation systems, a significant soil carbon sequestration was observed over the year of conversion from conventional to no-till practices, with a mean rate of 0.41 mg c ha( ...201223011303
integrated ecological risk assessment of pesticides in tropical ecosystems: a case study with carbofuran in brazil.the aim of the present study is to contribute an ecologically relevant assessment of the ecotoxicological effects of pesticide applications in agricultural areas in the tropics, using an integrated approach with information gathered from soil and aquatic compartments. carbofuran, an insecticide/nematicide used widely on sugarcane crops, was selected as a model substance. to evaluate the toxic effects of pesticide spraying for soil biota, as well as the potential indirect effects on aquatic biota ...201222068639
oviposition behavior of diatraea saccharalis (lepidoptera: crambidae) on different rice cultivars in louisiana.the sugarcane borer, diatraea saccharalis (f.), is a pest of a variety of graminaceous crops in the southern united states, including sugarcane, maize, and rice in louisiana. this study examined several aspects of d. saccharalis oviposition behavior on rice (oryza sativa). the vertical distribution of egg masses on four phenological stages of rice in the field showed that d. saccharalis prefers to oviposit on the uppermost portions of rice plants, regardless of plant stage. in greenhouse choice ...201222732615
the nearctic-caribbean species leptotrachelus dorsalis (fabricius, 1801): larval descriptions with a diagnosis of immature ctenodactylini and natural history notes on the genus and tribe (coleoptera, carabidae).adults and larvae of leptotrachelus dorsalis (fabricius), the sugarcane savior beetle, live in association with grasses, the larvae in the appressed leaf axils. both adult and larval leptotrachelus dorsalis eat larvae of the sugarcane borer, diatraea saccharalis (fabricius), and perhaps other insects living in the confines of the leaf sheaths of that and other grass-like species. the geographic range of leptotrachelus dorsalis extends from kansas in the west to the atlantic seaboard, north as fa ...201222679382
sugarwin: a sugarcane insect-induced gene with antipathogenic activity.in sugarcane fields, colonization of the stalk by opportunistic fungi usually occurs after the caterpillar diatraea saccharalis attacks the sugarcane plant. plants respond to insect attack by inducing and accumulating a large set of defense proteins. two homologues of a barley wound-inducible protein (barwin), sugarcane wound-inducible proteins sugarwin1 and sugarwin2, have been identified in sugarcane by an in silico analysis. antifungal properties have been described for a number of barwin hom ...201222250584
microarray analysis of global gene regulation in the cry1ab-resistant and cry1ab-susceptible strains of diatraea saccharalis.extensive adoption of transgenic bt corn in recent years for stalk borer control has increased risk of resistance evolution in the target pest populations. a bt-resistant strain of the sugarcane borer, diatraea saccharalis, was approximately 100-fold more tolerant to cry1ab toxin than the susceptible counterpart. to gain a better understanding of the molecular mechanisms of bt resistance, the cry1ab-susceptible (cry1ab-ss) and cry1ab-resistant (cry1ab-rr) strains of d. saccharalis were subjected ...201222228544
feasibility of using an alternative larval host and host plants to establish cotesia flavipes (hymenoptera: braconidae) in the temperate louisiana sugarcane ecosystem.attempts to establish cotesia flavipes cameron (hymenoptera: braconidae) in louisiana sugarcane fields to control the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae) have been unsuccessful. experiments were conducted to investigate the feasibility of using an alternative larval host and host plants to overcome barriers preventing establishment. in addition, we evaluated c. flavipes' ability to search for d. saccharalis in sugarcane without above-ground internodes. diatraea ev ...201222506999
elephant grass, sugarcane, and rice bran in diets for confined sheep.we aimed to evaluate the effects of diets, based on elephant grass or sugarcane as roughage and corn meal or rice bran as energy concentrate, on performance and body composition in terms of diet intake and digestibility. a total of 30 santa ines crossbreds (sic), castrated male sheep with 19.8 ± 2.0 kg initial body weight (bw) were used. six animals were slaughtered at the onset of the experiment to estimate the initial body composition for the other animals. the remaining 24 animals were distri ...201222528534
vermicomposting of milk processing industry sludge spiked with plant wastes.this work illustrates the vermistabilization of wastewater sludge from a milk processing industry (mpis) unit spiked with cow dung (cd), sugarcane trash (st) and wheat straw (ws) employing earthworms eisenia fetida. a total of nine experimental vermibeds were established and changes in chemical parameters of waste material have been observed for 90 days. vermistabilization caused significant reduction in ph, organic carbon and c:n ratio and substantial increase in total n, available p and exchan ...201222609678
influence of different supplements and sugarcane (saccharum officinarum l.) cultivars on intake, digestible variables and methane production of dairy heifers under tropical conditions.the sulphur hexafluoride (sf(6)) gas tracer method was used to measure methane (ch(4)) production of crossbred (3/4 holstein x zebu) dairy heifers fed two types of sugarcane (saccharum officinarum l.; cultivar iac-862480 (cc1) or cultivar iac-873184 (cc2)) and supplemented with urea or concentrate. the study was performed at embrapa southeast cattle, são carlos, sp, brazil, using a completely randomised design. differences between treatments were significant for digestibility of dry matter, orga ...201222476735
short communication: nutrient utilization of fresh sugarcane-based diets with slow-release nonprotein nitrogen addition for control-fed dairy heifers.an experiment was conducted to determine the effect of replacing soybean protein (sbm) with a slow-release urea (sr-u) in control-fed dairy heifers in the tropics. eight holstein heifers (237.6±5.45 kg of body weight) were allocated to 2 treatments in a crossover design. treatments were control (sbm) and sr-u (optigen ii; alltech inc., nicholasville, ky). the forage-to-concentrate ratio was 50:50 [dry matter (dm) basis], and fresh chopped sugarcane was the sole source of forage. data were analyz ...201222192216
dietary nitrate supplementation reduces methane emission in beef cattle fed sugarcane-based diets.the objective of this study was to determine the effect of dietary nitrate on methane emission and rumen fermentation parameters in nellore × guzera (bos indicus) beef cattle fed a sugarcane based diet. the experiment was conducted with 16 steers weighing 283 ± 49 kg (mean ± sd), 6 rumen cannulated and 10 intact steers, in a cross-over design. the animals were blocked according to bw and presence or absence of rumen cannula and randomly allocated to either the nitrate diet (22 g nitrate/kg dm) o ...201222287674
use of simple sequence repeat markers for dna fingerprinting and diversity analysis of sugarcane (saccharum spp) cultivars resistant and susceptible to red rot.red rod is an economically important disease of sugarcane caused by the fungus colletotrichum falcatum. we used a simple sequence repeat (ssr)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in pakistan. twenty-one highly polymorphic ssr markers were used for dna fingerprinting and genetic diversity analysis of 20 sugarcane cultivars. these ssr markers were found to be highly robust; we identified 144 alleles, with ...201222614346
rna interference of endochitinases in the sugarcane endophyte trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.the sugarcane root endophyte trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including n-acetyl-ß-d-glucosaminidases, chitobiosidases and endochitinases. we used targeted gene deletion and rna-dependent gene silencing strategies to disrupt n-acetyl-ß-d-glucosaminidase and endochitinase activities of the ...201223110120
occupational exposure to pesticides and parkinson's disease: a systematic review and meta-analysis of cohort studies.to systematically review available cohort studies and estimate quantitatively the association between occupational exposure to pesticides and parkinson's disease (pd).201222698719
evaluation of in vitro and in vivo effects of semipurified proteinase inhibitors from theobroma seeds on midgut protease activity of lepidopteran pest insects.we have characterized in vitro and in vivo effects of trypsin inhibitors from theobroma seeds on the activity of trypsin- and chymotrypsin-like proteins from lepidopteran pest insects. the action of semipurified trypsin inhibitors from theobroma was evaluated by the inhibition of bovine trypsin and chymotrypsin activities determined by the hydrolysis of n-benzoyl-dl-arginine-p-nitroanilide (bapa) and n-succinyl-ala-ala-pho-phe p-nitroanilide (s-(ala)2prophe-pna). proteinase inhibitor activities ...201222806759
moisture source and diet affect development and reproduction of orius thripoborus and orius naivashae, two predatory anthocorids from southern africa.the effect of moisture source and diet on the development and reproduction of the pirate bugs, orius thripoborus (hesse) and orius naivashae (poppius) (hemiptera: anthocoridae) was examined in the laboratory. both species had been collected in and around sugarcane fields in south africa. supplementing eggs of the flour moth ephestia kuehniella (zeller) (lepidoptera: pyralidae) with a green bean pod as a moisture source yielded better nymphal survival and faster development, as compared with free ...201222935002
enzymatic activity and proteomic profile of class iii peroxidases during sugarcane stem development.class iii peroxidases are present as large multigene families in all land plants. this large number of genes together with the diversity of processes catalyzed by peroxidases suggests possible functional specialization of each isoform. however, assigning a precise role for each individual peroxidase gene has continued to be a major bottleneck. here we investigated the enzyme activity and translational profile of class iii peroxidases during stem development of sugarcane as a first step in the es ...201222551762
the treatment of jaundice with medicinal plants in indigenous communities of the sub-himalayan region of uttarakhand, india.inspite of tremendous advances made in allopathic medical practices, herbs still play an important role in the management of various liver diseases. a large number of plants and formulations have been claimed to have hepatoprotective activity. jaundice is a symptom, indicative of the malfunctioning of the liver. this paper provides ethnomedicinal information on the plants used to treat jaundice by three important indigenous communities, i.e., nomadic gujjars, tharu and bhoxa of sub-himalayan reg ...201222759701
unintended consequences of bioethanol feedstock choice in china.economic, energy, and environmental impacts of 11 types of bioethanol feedstock in china were evaluated using a mixed-unit input-output life cycle assessment model. corn grain and wheat grain had higher negative economic, energy, and environmental impacts. sweet sorghum, cassava, sugar beet, and sugarcane showed better economic performance but increasing negative energy and environmental impacts. cellulose-based feedstocks in general showed positive economic, energy, and environmental performanc ...201223070140
use of plant residues on growth of mycorrhizal seedlings of neem (azadirachta indica a. juss.).owing to its multiple uses in veterinary medicine, biofertilizers, pest control, etc., the commercial cultivation of neem (azadirachta indica) has been increasing in various countries. the use of arbuscular mycorrhizal fungi (amf) and plant by-products (composted leaves and residues of neem and sugarcane) for the propagation of seedlings can be an efficient alternative to stimulate plant growth, reducing the propagation time and conferring increased tolerance of plants to biotic and abiotic stre ...201225363647
measurement and analysis of the mannitol partition coefficient in sucrose crystallization under simulated industrial conditions.mannitol is a major deterioration product of leuconstoc mesenteroides bacterial metabolism of sucrose and fructose from both sugarcane and sugar beet. the effect of crystallization conditions on the mannitol partition coefficient (k(eff)) between impure sucrose syrup and crystal has been investigated in a batch laboratory crystallizer and a batch pilot plant-scale vacuum pan. laboratory crystallization was operated at 65.5°c (150°f), 60.0°c (140°f), and 51.7°c (125°f) with a 78.0 brix (% refract ...201222632769
rapid and simple dna extraction method for the detection of enterotoxigenic staphylococcus aureus directly from food samples: comparison of pcr and lamp methods.the study describes the development of simple and rapid dna extraction method in combination with loop-mediated isothermal amplification (lamp) to detect enterotoxigenic staphylococcus aureus in food samples.201222519925
genetic variability and potential recombination events in the hc-pro gene of sugarcane streak mosaic virus.sugarcane streak mosaic virus (scsmv), a member of the family potyviridae, is an important viral pathogen affecting sugarcane production in india. the variability in the nucleotide (nt) and amino acid (aa) sequences of helper component proteinase (hc-pro) of scsmv isolates from india was investigated and compared with those of previously published virus isolates from different asian countries. comparison of all of the sequenced virus isolates revealed a high level of diversity in the hc-pro gene ...201222481599
blue native-page analysis of trichoderma harzianum secretome reveals cellulases and hemicellulases working as multienzymatic complexes.plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergistically for polysaccharide degradation. here, we present evidence that the fungus trichoderma harzianum, cultivated in medium containing the agricultural residue sugarcane bagasse, is ...201222744980
molecular characterization and phylogenetic analysis of sugarcane yellow leaf virus isolates from china.sugarcane yellow leaf virus (scylv) (genus polerovirus, family luteoviridae), the causal agent of sugarcane yellow leaf disease (yld), was first detected in china in 2006. to assess the distribution of scylv in the major sugarcane-growing chinese provinces, leaf samples from 22 sugarcane clones (saccharum spp. hybrid) showing yld symptoms were collected and analyzed for infection by the virus using reverse transcription pcr (rt-pcr), quantitative rt-pcr, and immunological assays. a complete geno ...201222752564
putative recombination signature and significance of insertion/deletion events in the rna-dependent rna polymerase coding region of sugarcane yellow leaf virus.the 5898 nucleotide single-strand rna genome of sugarcane yellow leaf virus (scylv) contains one long open reading frame, which is translated into a 120.6 kda polyprotein. the sequences of scylv isolates from the two scylv-susceptible cultivars from hawaii had a deletion of 48-51 nt in orf1. scylv from 12 sugarcane hybrid cultivars from different origins were tested by rt-pcr using a specific set of primers, to investigate the genome segment for this deletion. only three cultivars were found not ...201222542996
haplotype structure around bru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars.modern sugarcane cultivars (saccharum spp., 2n = 100-130) are high polyploid, aneuploid and of interspecific origin. a major gene (bru1) conferring resistance to brown rust, caused by the fungus puccinia melanocephala, has been identified in cultivar r570. we analyzed 380 modern cultivars and breeding materials covering the worldwide diversity with 22 molecular markers genetically linked to bru1 in r570 within a 8.2 cm segment. our results revealed a strong ld in the bru1 region and strong assoc ...201222572763
halopriming mediated salt and iso-osmotic peg stress tolerance and, gene expression profiling in sugarcane (saccharum officinarum l.).seed priming is a well known pre-germination strategy that improves seed performance. however, biochemical and molecular mechanisms underlying priming mediated stress tolerance are little understood. here, we report results of the study on growth, physiological characteristics and expression of stress responsive genes in salt primed sugarcane cv. co 86032 plants in response to salt (nacl, 150 mm) or iso-osmotic (-0.7 mpa) polyethylene glycol-peg 8000 (20 % w/v) stress exposure for 15 days. varia ...201222740137
enrichment of genomic dna for polymorphism detection in a non-model highly polyploid crop plant.large polyploid genomes of non-model species remain challenging targets for dna polymorphism discovery despite the increasing throughput and continued reductions in cost of sequencing with new technologies. for these species especially, there remains a requirement to enrich genomic dna to discover polymorphisms in regions of interest because of large genome size and to provide the sequence depth to enable estimation of copy number. various methods of enriching dna have been utilised, but some re ...201222624722
development of novel chloroplast microsatellite markers for miscanthus species (poaceae).a set of novel chloroplast microsatellite markers (cpssrs) was developed for the bioenergy crop miscanthus, and their utility in cross-species amplification was evaluated.201222615308
nonpoint source of nutrients and herbicides associated with sugarcane production and its impact on louisiana coastal water quality.a watershed analysis of nonpoint-source pollution associated with sugarcane (saccharum officinarum l.) production was conducted. runoff water samples following major rainfall events from two representative sugarcane fields (sc1 and sc2) were collected and analyzed. the impact of runoff on two receiving water bodies, st. james canal (sjc) and bayou chevreuil (bc) in a drainage basin (baratarian basin), was studied. results show that runoff flow/rainfall ratios at the sc1 were significantly higher ...201218948481
on-line identification of further flavone c- and o-glycosides from sugarcane (saccharum officinarum l., gramineae) by hplc-uv-ms.hplc-uv-ms (apci-ms/ms and cid/ms) was utilised for the identification of eight additional flavone glycosides from sugarcane (saccharum officinarum l., gramineae) extracts of bagasse, leaves and juice ('garapa'). relevant information about substitution patterns was obtained through uv detection using post-column addition of shift reagents, while tandem ms provided structural information confirming the proposed structures of the c-glycosides vitexin, orientin, luteolin-8-c-(rhamnosylglucoside), 4 ...201217019935
long-term water quality trends after implementing best management practices in south florida.a mandatory best management practices (bmp) program was implemented in the everglades agricultural area (eaa) farms basin-wide in 1995 as required by the everglades forever act to reduce p loads in drainage water reaching the everglades ecosystem. all farms in the eaa basin implement similar bmps, and basin wide p load reductions have exceeded the 25% reduction required by law; however, differences remain in water quality between subbasins. our objective was to determine long-term trends in p lo ...201319549945
modeling of phosphorus loads in sugarcane in a low-relief landscape using ontology-based simulation.water flow and p dynamics in a low-relief landscape manipulated by extensive canal and ditch drainage systems were modeled utilizing an ontology-based simulation model. in the model, soil water flux and processes between three soil inorganic p pools (labile, active, and stable) and organic p are represented as database objects. and user-defined relationships among objects are used to automatically generate computer code (java) for running the simulation of discharge and p loads. our objectives w ...201321043280
phosphorus speciation in sequentially extracted agro-industrial by-products: evidence from x-ray absorption near edge structure spectroscopy.the phosphorus (p) in agro-industrial by-products--a potential source of freshwater eutrophication but also a valuable fertilizer--needs to be speciated to evaluate its fate in the environment. we investigated to what extent x-ray absorption near edge structure (xanes) spectroscopy at the p k- and l2.3-edges reflected differences in sequentially extracted filter cakes from sugarcane (saccharum officinarum l.) (fic) and niger seed (guizotia abyssinica cass.; nic) processing industry in ethiopia. ...201321284316
in vitro selection and characterization of polyethylene glycol (peg) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (saccharum officinarum l.).a system for in vitro selection of drought tolerant callus lines in sugarcane was developed. high molecular weight peg was used as selective agent. selected callus line grew better than non-selected callus when grown on different concentrations of peg. the activity of antioxidant enzymes like cat, pox, apx and sod were high in selected callus than in non-selected callus. osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at high ...201324431494
cloning, expression and characterization of sugarcane (saccharum officinarum l.) transketolase.pentose phosphate pathway (ppp) composed of two functionally-connected phases, the oxidative and non-oxidative phase. both phases catalysed by a series of enzymes. transketolase is one of key enzymes of non-oxidative phase in which transfer two carbon units from fructose-6-phosphate to erythrose-4-phosphate and convert glyceraldehyde-3-phosphate to xylulose-5-phosphate. in plant, erythrose-4-phosphate enters the shikimate pathway which is produces many secondary metabolites such as aromatic amin ...201324132392
involvement of anthocyanins in the resistance to chilling-induced oxidative stress in saccharum officinarum l. leaves.whether anthocyanins elevate resistance to chilling-induced oxidative stress in saccharum officinarum l. cv badila seedlings is investigated. plants with four fully expanded leaves were exposed to chilling stress (8 °c/4 °c, 11 h photoperiod) for 3 days and then transferred to rewarming condition (25 °c/20 °c, 11 h photoperiod) for another 2 days. at the end of the chilling period, h2o2 and superoxide radical (o2-) levels increased sharply and were near the same in the central (cl) and the final ...201323932150
an-overview on invertase in sugarcane.saccharum officinarum is one of the most cultivated hybrid varieties among the sugarcane varieties. in sugarcane plant sucrose is the major carbohydrate which can be stored and transported. different physiological and biochemical studies on this crop report that invertase activity and sucrose concentration some how are key limiting step in the process of sucrose accumulation. significant efforts have been made in relation to the sucrose cycle by altering the sucrose phosphate synthetase, sucrose ...201323847400
comparative feedstock analysis in setaria viridis l. as a model for c4 bioenergy grasses and panicoid crop species.second generation feedstocks for bioethanol will likely include a sizable proportion of perennial c4 grasses, principally in the panicoideae clade. the panicoideae contain agronomically important annual grasses including zea mays l. (maize), sorghum bicolor (l.) moench (sorghum), and saccharum officinarum l. (sugar cane) as well as promising second generation perennial feedstocks including miscanthus×giganteus and panicum virgatum l. (switchgrass). the underlying complexity of these polyploid gr ...201323802002
short-term effects of sugarcane waste products from ethanol production plant as soil amendments on sugarcane growth and metal stabilization.numerous waste products have been widely studied and used as soil amendments and metal immobilizing agents. waste utilization from ethanol production processes as soil amendments is one of the most promising and sustainable options to help utilize materials effectively, reduce waste disposal, and add value to byproducts. as a consequence, this present work carried out a four-month pot experiment of sugarcane (saccharum officinarum l.) cultivation in cd and zn contaminated soil to determine the e ...201323511210
sugarcane bagasse treated with hydrous ferric oxide as a potential adsorbent for the removal of as(v) from aqueous solutions.the mechanism of as(v) removal from aqueous solutions by means of hydrated ferric oxide (hfo)-treated sugarcane bagasse (scb-hfo) (saccharum officinarum l.) was investigated. effects of different parameters, such as ph value, initial arsenic concentration, adsorbent dosage, contact time and ionic strength, on the as(v) adsorption were studied. the adsorption capacity of scb-hfo for as(v) was found to be 22.1 mg/g under optimum conditions of ph 4, contact time 3h and temperature 22 °c. initial as ...201323265467
tissue-specific expression and functional role of dehydrins in heat tolerance of sugarcane (saccharum officinarum).studies on the functional roles of dehydrins (dhns) in heat tolerance of plants are scarce. this study was conducted to immunohistolocalize dhns in leaves of heat-tolerant (cp-4333) and heat-sensitive (hsf-240) sugarcane (saccharum officinarum l.) clones at three phenological stages in order to elucidate their putative roles under heat stress. cp-4333 indicated greater amounts of heat-stable proteins than hsf-240 under heat stress. western blotting revealed the expression of three dhns in cp-433 ...201322886313
policosanol modulates hmg-coa reductase activity in cultured fibroblasts.cholesterol biosynthesis is strictly controlled by 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase.201311282173
enhanced cellulase production by trichoderma viride in a rotating fibrous bed bioreactor.filamentous fungi are widely used to produce cellulase, but how the fermentation conditions affect their production is not well known. in this study, cellulase production by trichoderma viride in submerged fermentations with free cells in a stirred-tank reactor (str) and immobilized cells in a rotating fibrous-bed bioreactor (rfbb) were investigated. compared to free-cell fermentation, immobilized-cell fermentation gave 35.5% higher fpase activity and 69.7% higher saccharification yield of sugar ...201323428816
first report of trypanosoma vivax outbreak in dairy cattle in são paulo state, brazil.this is the first description of a trypanosoma vivax outbreak in the state of são paulo (municipality of lins). fever, jaundice, decreased milk production, weight loss, profuse diarrhea, abortion, anemia, leukocytosis and hyperfibrinogenemia were observed in the affected animals. thirty-one cows and calves died out of a total of 1080 in the herd. three cows showed neurological symptoms like dysmetria, ataxia, muscle weakness, ptyalism, lymph node enlargement and submandibular edema. flagellated ...201322832751
xanthomonas albilineans ompa1 appears to be functionally modular and both the omc and c-like domains are necessary for leaf scald disease of sugarcane.several ez-tn5 insertions in gene locus xalc_0557 (ompa1) of the sugarcane leaf scald pathogen xanthomonas albilineans xafl07-1 were previously found to strongly affect pathogenicity and endophytic stalk colonization. xalc_0557 has a predicted ompa n-terminal outer membrane channel (omc) domain and an ompa c-like domain. further analysis of mutant m468, with an ez-tn5 insertion in the upstream omc domain coding region, revealed impaired epiphytic and endophytic leaf survival, impaired resistance ...201323758144
production and partial characterization of cellulases and xylanases from trichoderma atroviride 676 using lignocellulosic residual biomass.trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. best results were obtained when 3.0 % (w/v) untreated sugarcane bagasse was used (61.3 u ml(-1) for xylanase, 1.9 u ml(-1) for endoglucanase, 0.25 u ml(-1) for fpase, and 0.17 u ml(-1) for β-glucosidase) after 3-4 days fermentation. the maximal enzymatic activity for endoglucanase, fpase, and xylanase were observed at 50-60 °c and ph 4.0-5 ...201323306885
a novel promising trichoderma harzianum strain for the production of a cellulolytic complex using sugarcane bagasse in natura.brazil is a major producer of agro-industrial residues, such as sugarcane bagasse, which could be used as raw material for microbial production of cellulases as an important strategy for the development of sustainable processes of second generation ethanol production. for this purpose, this work aimed at screening for glycosyl hydrolase activities of fungal strains isolated from the brazilian cerrado. among 13 isolates, a trichoderma harzianum strain (l04) was identified as a promising candidate ...201324349958
understanding the cellulolytic system of trichoderma harzianum p49p11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-l-arabinofuranosidase.supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. this study investigated which enzymes were complementary to the enzyme set of trichoderma harzianum in the degradation of sugarcane bagasse. specific activities of t. harzianum extract on different substrates were compared with the extracts of penicillium echinulatum and trichoderma reesei, and two commercial cellulase preparations. comp ...201323391738
experimental mixture design as a tool to enhance glycosyl hydrolases production by a new trichoderma harzianum p49p11 strain cultivated under controlled bioreactor submerged fermentation.this work investigates the glycosyl hydrolase (gh) profile of a new trichoderma harzianum strain cultivated under controlled bioreactor submerged fermentation. the influence of different medium components (delignified steam-exploded sugarcane bagasse, sucrose, and soybean flour) on gh biosynthesis was assessed using experimental mixture design (emd). additionally, the effect of increased component concentrations in culture media selected from the emd was studied. it was found that that a mixed c ...201323265822
molecular characterization of indian sugarcane streak mosaic virus isolate.sugarcane streak mosaic virus (scsmv), a member of the genus poacevirus, family potyviridae, is an important viral pathogen affecting sugarcane cultivation in india. the complete nucleotide sequence of a scsmv isolate from india (scsmv-ind) was determined. the linear, assembled, single-stranded positive-sense rna genome of scsmv-ind was 9,786 nucleotides in length (excluding the poly (a) tail) and encoded a polyprotein of 3,131 amino acid residues. the genome of scsmv-ind shared high degree of s ...201323011777
phenolic content, antioxidant and antibacterial activity of selected natural sweeteners available on the polish market.seventeen natural sweeteners available on the polish market were screened for total phenolic content, by the folin-ciocalteu method, and for antioxidant activity, using the ferric reducing antioxidant power (frap) assay and the 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) radical cation decolorization assay (abts(·+)). in addition, we analyzed antibacterial activities against staphylococcus aureus strains: both those susceptible and those resistant to methicillin (mrsa). the results of ...201324007486
combined linkage and association mapping reveals candidates for scmv1, a major locus involved in resistance to sugarcane mosaic virus (scmv) in maize.sugarcane mosaic virus (scmv) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. maize resistance to scmv is associated with two dominant genes, scmv1 and scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. we combined both linkage and association mapping to identify positional candidate genes for scmv1.201324134222
proteomic and phytohormone analysis of the response of maize (zea mays l.) seedlings to sugarcane mosaic virus.sugarcane mosaic virus (scmv) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. control strategies have been developed, but only marginal successes have been achieved. for the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required.201323894637
comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus.sugarcane mosaic virus (scmv) is an important viral pathogen and has caused serious losses in grain and forage yield. to identify candidate scmv resistance proteins and to explore the molecular mechanisms involved in the plant-scmv interaction, we conducted proteomic analyses of leaf samples from resistant and susceptible ecotypes of maize infected with scmv. proteins were analyzed by quantitative two-dimensional differential gel electrophoresis (2d-dige), and 93 protein spots showed statistical ...201323770298
genetic diversity and population structure of sugarcane mosaic virus.sugarcane mosaic virus (scmv) is one of the causal pathogens of mosaic diseases on sugarcane, maize, sorghum and some other graminaceous species with worldwide distribution. the global genetic diversity and molecular evolution of scmv capsid protein (cp) gene were investigated with the nucleotide sequences available in the genbank database. phylogenetic analyses revealed that scmv isolates clustered in relation to their original hosts, and geographically distinct isolates from maize or sugarcane ...201323116592
sugarcane vinasse: environmental implications of its use.the inadequate and indiscriminate disposal of sugarcane vinasse in soils and water bodies has received much attention since decades ago, due to environmental problems associated to this practice. vinasse is the final by-product of the biomass distillation, mainly for the production of ethanol, from sugar crops (beet and sugarcane), starch crops (corn, wheat, rice, and cassava), or cellulosic material (harvesting crop residues, sugarcane bagasse, and wood). because of the large quantities of vina ...201324084103
cellulase production from a new strain acremonium strictum isolated from the brazilian biome using different substrates.the objective of the present study was to evaluate the production of cellulolytic enzymes by acremonium strictum isolated from brazilian biome using different substrates. fermentations were initially carried out using commercial substrates, including microcrystalline cellulose (avicel® and servacel®) and carboxymethylcellulose (cmc). this was followed by fermentations performed using lignocellulosic biomass: sugarcane bagasse pretreated at different intensities. the fermentations were carried ou ...201323186655
exploring grape marc as trove for new thermotolerant and inhibitor-tolerant saccharomyces cerevisiae strains for second-generation bioethanol production.robust yeasts with high inhibitor, temperature, and osmotic tolerance remain a crucial requirement for the sustainable production of lignocellulosic bioethanol. these stress factors are known to severely hinder culture growth and fermentation performance.201324286305
design and optimization of a process for sugarcane molasses fermentation by saccharomyces cerevisiae using response surface methodology.a statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolated saccharomyces cerevisiae y-39. response surface methodology rsm based on central composite face centered design ccfd was employed to statistically evaluate and optimize the conditions for maximum bioethanol production and study the significance and interaction of incubation period, initial ph, incubation temperature, and molasses concent ...201324222769
health impact assessment of liquid biofuel production.bioethanol and biodiesel as potential substitutes for fossil fuels in the transportation sector have been analyzed for environmental suitability. however, there could be impacts on human health during the production, therefore adverse health effects have to be analyzed. the aim of this study is to analyze to what health risk factors humans are exposed to in the production of biofuels and what the size of the health effects is. a health impact assessment expressed as disability adjusted life year ...201322774773
detection of added beet sugar in concentrated and single strength fruit juices by deuterium nuclear magnetic resonance (snif-nmr method): collaborative study.a collaborative study of the site-specific natural isotope fractionation-nuclear magnetic resonance (snif-nmr) method for detecting added beet sugar in fruit juices is reported. this method is complementary to the stable carbon isotope ratio analysis (scira) (aoac official methods 981.09 and 982.21), which can detect sugars derived from plants exhibiting c4 metabolism (corn and sugarcane). it is based on the fact that the deuterium content at specific positions of the sugar molecules is higher i ...20138757451
circadian rhythms of sense and antisense transcription in sugarcane, a highly polyploid crop.commercial sugarcane (saccharum hybrid) is a highly polyploid and aneuploid grass that stores large amounts of sucrose in its stem. we have measured circadian rhythms of sense and antisense transcription in a commercial cultivar (rb855453) using a custom oligoarray with 14,521 probes that hybridize to sense transcripts (ss) and 7,380 probes that hybridize to antisense transcripts (as).we estimated that 32% of ss probes and 22% as probes were rhythmic. this is a higher proportion of rhythmic prob ...201323936527
centesimal composition and physical-chemistry analysis of the edible mushroom lentinus strigosus occurring in the brazilian amazon.the centesimal composition and the physical and chemical analyses of lentinus strigosus, an edible mushroom occurring in the brazilian amazon and produced in alternative substrates based on wood and agroindustrial residues, were evaluated. for this purpose, the c, n, ph, soluble solids, water activity, protein, lipids, total fiber, ash, carbohydrate, and energy levels were determined. the substrates were formulated from simarouba amara aubl. ("marupá"), ochroma piramidale cav. ex. lam. ("pau-de- ...201324141410
chemical shift assignments of the canecystatin-1 from saccharum officinarum.cystatins are cysteine proteases inhibitors that are widely distributed among insects, mammalians and plants. here we report the complete resonance assignment of canecystatin-1 from saccharum officinarum obtained by heteronuclear multidimensional high-resolution nuclear magnetic resonance spectroscopy. the consensus chemical shift index was calculated and showed the presence of one α-helix (residues 27-43) and three β-strands (residues 48-74, 78-89 and 94-104), a secondary structure pattern that ...201322696137
the influence of total solids content and initial ph on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes.hydrogen is a valuable clean energy source, and its production by biological processes is attractive and environmentally sound and friendly. in méxico 5 million tons/yr of agroindustrial wastes are generated; these residues are rich in fermentable organic matter that can be used for hydrogen production. on the other hand, batch, intermittently vented, solid substrate fermentation of organic waste has attracted interest in the last 10 years. thus the objective of our work was to determine the eff ...201323732191
differential regulation of defense-related gene expression in response to red rot pathogen colletotrichum falcatum infection in sugarcane.red rot is a serious disease of sugarcane caused by the fungus colletotrichum falcatum imposing a considerable economic loss annually in all sugarcane-producing countries. in this study, we analyzed the early resistance response of sugarcane to red rot fungus by comparing the differences between control and inoculated stalk tissues. differential display reverse transcription polymerase chain reaction (dd-rt-pcr) was employed to identify altered expression of genes in disease-resistant cv co 9300 ...201323861092
optimization of β-glucosidase, β-xylosidase and xylanase production by colletotrichum graminicola under solid-state fermentation and application in raw sugarcane trash saccharification.efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. the production of β-glucosidase, β-xylosidase and xylanase by colletotrichum graminicola was optimized using response surface methodology (rsm). maximal production occurred in wheat bran. sugarcane trash, peanut hulls and corncob enhanced β-glucosidase, β-xylosidase and xylanase production, respectively. maximal levels after optimization reached 159.3 ± 12.7 u g-1, 128.1 ...201323364611
predicting greenhouse gas emissions and soil carbon from changing pasture to an energy crop.bioenergy related land use change would likely alter biogeochemical cycles and global greenhouse gas budgets. energy cane (saccharum officinarum l.) is a sugarcane variety and an emerging biofuel feedstock for cellulosic bio-ethanol production. it has potential for high yields and can be grown on marginal land, which minimizes competition with grain and vegetable production. the daycent biogeochemical model was parameterized to infer potential yields of energy cane and how changing land from gra ...201323991028
fibrolytic enzyme and ammonia application effects on the nutritive value, intake, and digestion kinetics of bermudagrass hay in beef cattle.the objectives were to compare the effect of exogenous fibrolytic enzyme (biocellulase a20) or anhydrous ammonia (4% dm) treatment on the nutritive value, voluntary intake, and digestion kinetics of bermudagrass (cynodon dactylon cultivar coastal) hay harvested after 2 maturities (5- and 13-wk regrowths). six individually housed, ruminally cannulated brangus steers (bw 325 ± 10 kg) were used in an experiment with a 6 × 6 latin square design with a 3 (additives) × 2 (maturities) factorial arrange ...201323893992
short-term effects of silage volatile compounds on feed intake and digestion in beef cattle.two experiments were conducted to evaluate whether fermentation end products in silage affect intake and digestion in beef cattle. six rumen-cannulated nellore steers were randomly assigned to a replicated 3 × 3 latin square design with 14-d periods. each period consisted of 9 d for adaptation and 5 d for sample collection. steers were housed in a tie-stall barn and individually fed once daily at 0800 h. the dietary treatments in exp. 1 were as follows: 60% corn silage plus 40% concentrate (con) ...201323478815
comparative metagenomic analysis of microcosm structures and lignocellulolytic enzyme systems of symbiotic biomass-degrading consortia.decomposition of lignocelluloses by cooperative microbial actions is an essential process of carbon cycling in nature and provides a basis for biomass conversion to fuels and chemicals in biorefineries. in this study, structurally stable symbiotic aero-tolerant lignocellulose-degrading microbial consortia were obtained from biodiversified microflora present in industrial sugarcane bagasse pile (bgc-1), cow rumen fluid (crc-1), and pulp mill activated sludge (asc-1) by successive subcultivation o ...201323381385
recovery of bovine lysozyme from transgenic sugarcane stalks: extraction, membrane filtration, and purification.increased industrial use of sugarcane (saccharum spp. hybrid) for food and bioenergy has led to considerable improvements in its genetic transformation, which allowed the development of not only pest- and herbicide-resistant lines but also lines expressing high-value bioproducts and biopolymers. however, the economic benefits of using inexpensive transgenic plant systems for the production of industrial proteins could be offset by high downstream processing costs. in this work, transgenic sugarc ...201323329238
production of methanol from biomass waste via pyrolysis.the production of methanol from agricultural, forestry, livestock, poultry, and fishery waste via pyrolysis was investigated. pyrolysis was conducted in a tube furnace at 450-500 °c. sugarcane bagasse showed the methanol production (5.93 wt.%), followed by roots and sawdust with 4.36 and 4.22 wt.%, respectively. animal waste offered the lowest content of methanol, as only 0.46, 0.80, and 0.61 wt.% were obtained from fishery, goat, and cow waste, respectively. it was also observed that the percen ...201323266847
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