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development and mapping of snp assays in allotetraploid cotton.a narrow germplasm base and a complex allotetraploid genome have made the discovery of single nucleotide polymorphism (snp) markers difficult in cotton (gossypium hirsutum). to generate sequence for snp discovery, we conducted a genome reduction experiment (ecori, bafi double digest, followed by adapter ligation, biotin-streptavidin purification, and agarose gel separation) on two accessions of g. hirsutum and two accessions of g. barbadense. from the genome reduction experiment, a total of 2.04 ...201222252442
protein expression changes during cotton fiber elongation in response to low temperature stress.low temperature stress is one of the major abiotic stresses limiting the formation of cotton (gossypium hirsutum l.) fiber qualities, especially fiber length. to investigate the molecular adaptation mechanisms of cotton fiber elongation to low temperature stress, two cotton cultivars, kemian 1 (low temperature-tolerant) and sumian 15 (low temperature-sensitive), were planted in the field at two sowing dates (25 april and 10 june). the two sowing dates resulted in different growing conditions and ...201222244703
protein differential expression in the elongating cotton (gossypium hirsutum l.) fiber under nitrogen stress.nitrogen (n) is an essential macronutrient and an important factor limiting agricultural productivity. n deficient or excess conditions often occur during the cotton growth season and incorrect n application may affect cotton fiber yield and quality. here, the influence of n stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spectrometry. the results indicated that n application rate affects nitrogen accumulation in fiber cells and fiber length. ...201223160829
transcriptome profiling analysis reveals that flavonoid and ascorbate-glutathione cycle are important during anther development in upland cotton.previous transcriptome profiling studies have investigated the molecular mechanisms of pollen and anther development, and identified many genes involved in these processes. however, only 51 anther ests of upland cotton (gossypium hirsutum) were found in ncbi and there have been no reports of transcriptome profiling analyzing anther development in upland cotton, a major fiber crop in the word.201223155472
functional genomics of fuzzless-lintless mutant of gossypium hirsutum l. cv. mcu5 reveal key genes and pathways involved in cotton fibre initiation and elongation.fuzzless-lintless cotton mutants are considered to be the ideal material to understand the molecular mechanisms involved in fibre cell development. although there are few reports on transcriptome and proteome analyses in cotton at fibre initiation and elongation stages, there is no comprehensive comparative transcriptome analysis of fibre-bearing and fuzzless-lintless cotton ovules covering fibre initiation to secondary cell wall (scw) synthesis stages. in the present study, a comparative transc ...201223151214
development of a plant-based threshold for tarnished plant bug (hemiptera: miridae) in cotton.the tarnished plant bug, lygus lineolaris (palisot de beauvois), is an important pest of cotton, gossypium hirsutum l., in the mid-southern united states. it is exclusively controlled with foliar insecticide applications, and sampling methods and thresholds need to be revisited. the current experiment was designed to establish a plant-based threshold during the flowering period of cotton development. experiments were conducted in mississippi in 2005 and 2006, arkansas in 2005, and louisiana in 2 ...201223356065
separation of integument and nucellar tissues from cotton ovules (gossypium hirsutum l.) for both high- and low-throughput molecular applications.here we present a quick and low-cost method to separate the different layers of tissue from the ovules and young seeds of cotton (gossypium hirsutum l.) for use in high- and low-throughput molecular applications. this method is performed at room temperature using standard laboratory equipment and does not require embedding of the samples, time-consuming fixation, or micro-sectioning procedures. we show that the three main tissues can be efficiently separated from isolated ovules collected on the ...201223237485
growing season carbon dioxide exchange in flooded non-mulching and non-flooded mulching cotton.there is much interest in the role that agricultural practices might play in sequestering carbon to help offset rising atmospheric co₂ concentrations. however, limited information exists regarding the potential for increased carbon sequestration of different management strategies. the objective of this study was to quantify and contrast carbon dioxide exchange in traditional non-mulching with flooding irrigation (tf) and plastic film mulching with drip irrigation (pm) cotton (gossypium hirsutum ...201223226376
combining ability analysis for within-boll yield components in upland cotton (gossypium hirsutum l.).cotton is an important cash crop worldwide, accounting for a large percentage of world agricultural exports; however, yield per acre is still poor in many countries, including pakistan. diallel mating system was used to identify parents for improving within-boll yield and fiber quality parameters. combining ability analysis was employed to obtain suitable parents for this purpose. the parental genotypes cp-15/2, niab krishma, cim-482, ms-39, and s-12 were crossed in complete diallel mating under ...201223007974
global alteration of micrornas and transposon-derived small rnas in cotton (gossypium hirsutum) during cotton leafroll dwarf polerovirus (clrdv) infection.small rnas (srnas) are a class of non-coding rnas ranging from 20- to 40-nucleotides (nts) that are present in most eukaryotic organisms. in plants, srnas are involved in the regulation of development, the maintenance of genome stability and the antiviral response. viruses, however, can interfere with and exploit the silencing-based regulatory networks, causing the deregulation of srnas, including small interfering rnas (sirnas) and micrornas (mirnas). to understand the impact of viral infection ...201222987114
the draft genome of a diploid cotton gossypium raimondii.we have sequenced and assembled a draft genome of g. raimondii, whose progenitor is the putative contributor of the d subgenome to the economically important fiber-producing cotton species gossypium hirsutum and gossypium barbadense. over 73% of the assembled sequences were anchored on 13 g. raimondii chromosomes. the genome contains 40,976 protein-coding genes, with 92.2% of these further confirmed by transcriptome data. evidence of the hexaploidization event shared by the eudicots as well as o ...201222922876
molecular evolution and phylogenetic analysis of genes related to cotton fibers development from wild and domesticated cotton species in gossypium.the domestication of both diploid and tetraploid cotton species was carried out for fiber utilization. to understand the origin and domestication of fibers, 18 genes related to fiber development were individually cloned and sequenced from 22 different cotton species. their structures, phylogenetic relationship and molecular evolution were further studied. in the orthologous and homeologous loci of the 18 genes, the sequence and structure of 72.22% were conserved and 27.78% were diverse. tree top ...201222381639
inheritance of long staple fiber quality traits of gossypium barbadense in g. hirsutum background using csils.gossypium hirsutum is a high yield cotton species that exhibits only moderate performance in fiber qualities. a promising but challenging approach to improving its phenotypes is interspecific introgression, the transfer of valuable traits or genes from the germplasm of another species such as g. barbadense, an important cultivated extra long staple cotton species. one set of chromosome segment introgression lines (csils) was developed, where tm-1, the genetic standard in g. hirsutum, was used as ...201222297564
overexpression of ghsusa1 increases plant biomass and improves cotton fiber yield and quality.cotton (gossypium spp.) is an important economic crop and the largest source of textile fiber in the world. however, to date, only a few genes have been identified that exhibit critical roles in fiber development, and few has shown positive effects on fiber yield and quality in transgenic cotton. here, we report the characterization of a novel sucrose synthase (susa1) gene from a superior quality fiber germplasm line 7235 in gossypium hirsutum. by association analysis, ghsusa1 was highly correla ...201222044435
repeated polyploidization of gossypium genomes and the evolution of spinnable cotton fibres.polyploidy often confers emergent properties, such as the higher fibre productivity and quality of tetraploid cottons than diploid cottons bred for the same environments. here we show that an abrupt five- to sixfold ploidy increase approximately 60 million years (myr) ago, and allopolyploidy reuniting divergent gossypium genomes approximately 1-2 myr ago, conferred about 30-36-fold duplication of ancestral angiosperm (flowering plant) genes in elite cottons (gossypium hirsutum and gossypium barb ...201223257886
microsatellite loci for gossypium davidsonii (malvaceae) and other d-genome, sonoran desert endemic cotton species.microsatellite primers previously developed for domesticated cotton (gossypium hirsutum; tetraploid) were screened for their utility in investigating genetic structure and gene flow within g. davidsonii and five other wild, mexican, d-genome cotton species (all diploid).201222358044
formation of tetrahydrocurcumin by reduction of curcumin with cultured plant cells of marchantia polymorpha.cultured plant cells of marchantia polymorpha, nicotiana tabacum, phytolacca americana, catharanthus roseus, and gossypium hirsutum were examined for their ability to reduce curcumin. only m. polymorpha cells converted curcumin into tetrahydrocurcumin in 90% yield in one day. time-course experiment revealed a two-step formation of tetrahydrocurcumin via dihydrocurcumin.201222574459
effects of corn processing method and dietary inclusion of wet distillers grains with solubles on energy metabolism, carbon-nitrogen balance, and methane emissions of cattle.the growing ethanol industry in the southern great plains has increased the use of wet distillers grains with solubles (wdgs) in beef cattle (bos taurus) finishing diets. few studies have used steam-flaked corn (zea mays l.; sfc)-based diets to evaluate the effects of wdgs in finishing cattle diets, and a reliable estimate of the net energy value of wdgs has yet to be determined. effects of corn processing method and wdgs on energy metabolism, c and n balance, and enteric methane (ch(4)) product ...201222585790
lack of fitness costs associated with acetamiprid resistance in bemisia tabaci (hemiptera: aleyrodidae).sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), is a devastating pest that can cause severe damage to a range of crops by direct feeding and by plant virus transmission. because of indiscriminate use of insecticides, this whitefly has developed resistance to several insecticides, including neonicotinoids. our objectives were to determine fitness components affected by acetamiprid resistance in b. tabaci. assay results showed that selection with acetamiprid had removed ...201222928322
geminivirus-mediated delivery of florigen promotes determinate growth in aerial organs and uncouples flowering from photoperiod in cotton.plant architecture and the timing and distribution of reproductive structures are fundamental agronomic traits shaped by patterns of determinate and indeterminate growth. florigen, encoded by flowering locus t (ft) in arabidopsis and single flower truss (sft) in tomato, acts as a general growth hormone, advancing determinate growth. domestication of upland cotton (gossypium hirsutum) converted it from a lanky photoperiodic perennial to a highly inbred, compact day-neutral plant that is managed a ...201222615805
effects of entomopathogenic nematodes on evolution of pink bollworm resistance to bacillus thuringiensis toxin cry1ac.the evolution of resistance by pests can reduce the efficacy of transgenic crops that produce insecticidal toxins from bacillus thuringiensis (bt). however, fitness costs may act to delay pest resistance to bt toxins. meta-analysis of results from four previous studies revealed that the entomopathogenic nematode steinernema riobrave (rhabditida: steinernematidae) imposed a 20% fitness cost for larvae of pink bollworm, pectinophora gossypiella (saunders) (lepidoptera: gelechiidae), that were homo ...201222812141
impact of mine waste dumps on growth and biomass of economically important crops.the present study aimed to investigate the effect of magnesite and bauxite waste dumps on growth and biochemical parameters of some edible and economically important plants such as vigna radiata, v. mungo, v. unguiculata, eleusine coracana, cajanus cajan, pennisetum glaucum, macrotyloma uniflorum, oryza sativa, sorghum bicolour, sesamum indicum, ricinus communis, brassica juncea, gossypium hirsutum and jatropha curcas. the growth rate of all the crops was observed in the range of 75 to 100% in m ...201223741803
[construction and pathogenicity tests of a mutated specific fragment scf73 in verticillium dahliae].to identify preliminarily the specific fragment scf73's function in verticillium dahlia virulence.201223383504
genetic structure and gene flow among brazilian populations of heliothis virescens (lepidoptera: noctuidae).population genetic studies are essential to the better application of pest management strategies, including the monitoring of the evolution of resistance to insecticides and genetically modified plants. bacillus thuringiensis berliner (bt) crops have been instrumental in controlling tobacco budworm, heliothis virescens (f.) (lepidoptera: noctuidae), a pest that has developed resistance to many common insecticides once used for its management. in our study, microsatellite markers were applied to ...201223356080
[arachnofauna (araneae: araneae) in transgenic and conventional cotton crops (gossypium hirsutum) in the north of santa fe, argentina].spiders have considerable potential importance for their role as predators to some pests in agricultural systems. the composition of spiders in transgenic and conventional cotton at the research station of inta reconquista (santa fe) was studied during the 2005-2006 season. the experiment was a complete randomized block design with three replications and three treatments: transgenic bt cotton (albt), conventional cotton without chemical control (alcsc), and conventional cotton with chemical cont ...201223894933
variable rate application of nematicides on cotton fields: a promising site-specific management strategy.field tests were conducted to determine if differences in response to nematicide application (i.e., root-knot nematode (rkn) populations, cotton yield, and profitability) occurred among rkn management zones (mz). the mz were delineated using fuzzy clustering of five terrain (tr) and edaphic (ed) field features related to soil texture: apparent soil electrical conductivity shallow (eca-shallow) and deep (eca-deep), elevation (el), slope (sl), and changes in bare soil reflectance. zones with lowes ...201223482903
arsenophonus groel interacts with clcuv and is localized in midgut and salivary gland of whitefly b. tabaci.cotton leaf curl virus (clcuv) (gemininiviridae: begomovirus) is the causative agent of leaf curl disease in cotton plants (gossypium hirsutum). clcuv is exclusively transmitted by the whitefly species b. tabaci (gennadius) (hemiptera: alerodidae). b. tabaci contains several biotypes which harbor dissimilar bacterial endo-symbiotic community. it is reported that these bacterial endosymbionts produce a 63 kda chaperon groel protein which binds to geminivirus particles and protects them from rapid ...201222900008
ghwrky15, a member of the wrky transcription factor family identified from cotton (gossypium hirsutum l.), is involved in disease resistance and plant development.as a large family of regulatory proteins, wrky transcription factors play essential roles in the processes of adaptation to diverse environmental stresses and plant growth and development. although several studies have investigated the role of wrky transcription factors during these processes, the mechanisms underlying the function of wrky members need to be further explored, and research focusing on the wrky family in cotton crops is extremely limited.201222883108
genome-wide profiling of mirnas and other small non-coding rnas in the verticillium dahliae-inoculated cotton roots.micrornas (mirnas) and small interfering rnas (sirnas) are short (19-25 nucleotides) non-coding rna molecules that have large-scale regulatory effects on development and stress responses in plants. verticillium wilt is a vascular disease in plants caused by the fungal pathogen verticillium dahliae. the objective of this study is to investigate the transcriptional profile of mirnas and other small non-coding rnas in verticillium-inoculated cotton roots. four small rna libraries were constructed f ...201222558219
cotton ghmkk5 affects disease resistance, induces hr-like cell death, and reduces the tolerance to salt and drought stress in transgenic nicotiana benthamiana.mitogen-activated protein kinase (mapk) cascades are involved in various processes from plant growth and development to biotic and abiotic stress responses. mapk kinases (mapkks), which link mapks and mapkk kinases (mapkkks), play crucial roles in mapk cascades to mediate a variety of stress responses in plants. however, few mapkks have been functionally characterized in cotton (gossypium hirsutum). in this study, a novel gene, ghmkk5, from cotton belonging to the group c mapkks was isolated and ...201222442420
chilling stress--the key predisposing factor for causing alternaria alternata infection and leading to cotton (gossypium hirsutum l.) leaf senescence.leaf senescence plays a vital role in nutrient recycling and overall capacity to assimilate carbon dioxide. cotton premature leaf senescence, often accompanied with unexpected short-term low temperature, has been occurring with an increasing frequency in many cotton-growing areas and causes serious reduction in yield and quality of cotton. the key factors for causing and promoting cotton premature leaf senescence are still unclear. in this case, the relationship between the pre-chilling stress a ...201222558354
cell suspension culture-mediated incorporation of the rice bel gene into transgenic cotton.cotton plants engineered for resistance to the herbicides, glyphosate or glufosinate have made a considerable impact on the production of the crop worldwide. in this work, embryogenic cell cultures derived from gossypium hirsutum l. cv coker 312 hypocotyl callus were transformed via agrobacterium tumefaciens with the rice cytochrome p450 gene, cyp81a6 (bel). in rice, bel has been shown to confer resistance to both bentazon and sulfanylurea herbicides. transformed cells were selected on a liquid ...201222768325
overexpression of the atlos5 gene increased abscisic acid level and drought tolerance in transgenic cotton.drought is the major environmental stress that limits cotton (gossypium hirsutum l.) production worldwide. los5/aba3 (los5) encodes a molybdenum co-factor and is essential for activating aldehyde oxidase, which is involved in abscisic acid (aba) biosynthesis. in this study, a los5 cdna of arabidopsis thaliana was overexpressed in cotton cultivar zhongmiansuo35 (z35) by agrobacterium tumefaciens-mediated transformation. the transformation and overexpression of atlos5 were assessed by pcr and rt-p ...201222412184
molecular typing of mealybug phenacoccus solenopsis populations from different hosts and locations in punjab, india.true identity and existence of genetic variability in mealy bug from different regions holds immense significance for adopting appropriate control measures along with predicting the development of any biotypes. mealy bug, phenacoccus solenopsis adults were collected from four host plants i.e., gossypium hirsutum--cotton (c), abelmoschus esculentus--okra (o), pennisetum glaucum--napier bajra (b) and a weed--parthenium hysterophorus (p) in five cotton growing districts i.e., abohar, bathinda, mans ...201223029900
within-plant distribution of cotton aphid (hemiptera: aphididae) in cotton cultivars with colored fibers.we describe the vertical and horizontal distribution of the cotton aphid aphis gossypii glover within a cotton plant in two cotton (gossypium hirsutum linnaeus) cultivars (brs safira and brs rubí) with colored fiber over the time. measurements of aphid population dynamics and distribution in the cotton plants were recorded in intervals of seven days. the number of apterous or alate aphids and their specific locations were recorded, using as a reference point the location of nodes on the mainstem ...201222782536
aphid antixenosis in cotton is activated by the natural plant defence elicitor cis-jasmone.upon insect herbivory, plants can release blends of volatile organic compounds (vocs) that modify herbivore and natural enemy behaviour. we have shown recently that cotton, gossypium hirsutum, emits a blend of defence vocs that repels the cotton aphid, aphis gossypii, upon herbivory by this notorious crop pest, including (z)-3-hexenyl acetate, (e)-4,8-dimethyl-1,3,7-nonatriene (dmnt), methyl salicylate and (e,e)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (tmtt). in this study, we investigated cha ...201222516741
new insights into roles of cell wall invertase in early seed development revealed by comprehensive spatial and temporal expression patterns of ghcwin1 in cotton.despite substantial evidence on the essential roles of cell wall invertase (cwin) in seed filling, it remains largely unknown how cwin exerts its regulation early in seed development, a critical stage that sets yield potential. to fill this knowledge gap, we systematically examined the spatial and temporal expression patterns of a major cwin gene, ghcwin1, in cotton (gossypium hirsutum) seeds from prefertilization to prestorage phase. ghcwin1 messenger rna was abundant at the innermost seed coat ...201222864582
hemigossypol, a constituent in developing glanded cottonseed (gossypium hirsutum).gossypol is a dimeric sesquiterpenoid first identified in cottonseed, but found in various tissues in the cotton plant including the seed. from its first discovery, it was assumed that hemigossypol was the biosynthetic precursor of gossypol. previous studies established that peroxidase (either from horseradish or from cottonseed) converts hemigossypol to gossypol. however, hemigossypol has never been identified in healthy cottonseed. in a temporal study using hplc and lc-ms, hemigossypol was ide ...201222369216
ghagp31, a cotton non-classical arabinogalactan protein, is involved in response to cold stress during early seedling development.arabinogalactan proteins (agps), a superfamily of highly glycosylated hydroxyproline-rich glycoproteins, are widely implicated in plant growth and development. a gene (including its cdna), designated ghagp31, encoding a non-classical agp protein was isolated from cotton (gossypium hirsutum). the deduced ghagp31 protein contains the conserved features of non-classical agps: a putative signal peptide, n-terminal histidine-rich stretch, middle repetitive proline-rich domain and a cysteine-containin ...201222222112
characterization of rhizobacterial strain rs-2 with acc deaminase activity and its performance in promoting cotton growth under salinity stress.a plant growth-promoting rhizobacterial strain rs-2 with 1-aminocyclopropane-1-carboxylate (acc) deaminase activity was isolated from salinized soils using acc as the sole nitrogen source. based on its physiological and biochemical properties and 16s rdna sequence analysis, this strain was identified as raoultella planticola. the maximum value of nitrogen fixation, dissolved phosphorus and dissolved potassium of rs-2 were 148.8 μg/ml, 205.0 and 4.31 mg/l, respectively within 192 h liquid culture ...201222806112
overexpression of a cotton gene that encodes a putative transcription factor of ap2/erebp family in arabidopsis affects growth and development of transgenic plants.in the study, a gene encoding a putative ethylene response factor of ap2/erebp family was isolated from cotton (gossypium hirsutum) and designated as gherf12. sequence alignment showed that gherf12 protein contains a central ap2/erf domain (58 amino acids) with two functional conserved amino acid residues (ala14 and asp19). transactivation assay indicated that gherf12 displayed strong transcription activation activity in yeast cells, suggesting that this protein may be a transcriptional activato ...201324194949
ghcax3 gene, a novel ca(2+)/h(+) exchanger from cotton, confers regulation of cold response and aba induced signal transduction.as a second messenger, ca(2+) plays a major role in cold induced transduction via stimulus-specific increases in [ca(2+)]cyt, which is called calcium signature. during this process, caxs (ca(2+)/h(+) exchangers) play critical role. for the first time, a putative ca(2+)/h(+) exchanger ghcax3 gene from upland cotton (gossypium hirsutum cv. 'yz-1') was isolated and characterized. it was highly expressed in all tissues of cotton except roots and fibers. this gene may act as a regulator in cotton's r ...201323776653
cotton plasma membrane intrinsic protein 2s (pip2s) selectively interact to regulate their water channel activities and are required for fibre development.aquaporins are thought to be associated with water transport and play important roles in cotton (gossypium hirsutum) fibre elongation. among aquaporins, plasma membrane intrinsic proteins (pips) constitute a plasma-membrane-specific subfamily and are further subdivided into pip1 and pip2 groups. in this study, four fibre-preferential ghpip2 genes were functionally characterized. the selective interactions among ghpip2s and their interaction proteins were studied in detail to elucidate the molecu ...201323656428
a cotton gene encoding myb-like transcription factor is specifically expressed in pollen and is involved in regulation of late anther/pollen development.in flowering plants, pollen development is a highly programmed process, in which a lot of genes are involved. in this study, a gene, designated as ghmyb24, encoding r2r3-myb-like protein was isolated from cotton. ghmyb24 protein is localized in the cell nucleus and acts as a transcriptional activator. northern blot analysis revealed that ghmyb24 transcripts were predominantly detected in anthers. it was further found that strong expression of ghmyb24 was mainly detected in pollen and was regulat ...201323447105
a cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress.cotton (gossypium hirsutum), the most important textile crop worldwide, often encounters abiotic stress such as drought and waterlog during its growth season (summer), and its productivity is significantly limited by adverse factors. to investigate the molecular adaptation mechanisms of this plant species to abiotic stress, a gene encoding the plasma membrane intrinsic protein (pip) was isolated in cotton, and designated as ghpip2;7. quantitative reverse transcriptase polymerase chain reaction a ...201323178915
genetic transformation of cotton with a harpin-encoding gene hpaxoo confers an enhanced defense response against verticillium dahliae kleb.the soil-borne fungal pathogen verticillium dahliae kleb causes verticillium wilt in a wide range of crops including cotton (gossypium hirsutum). to date, most upland cotton varieties are susceptible to v. dahliae and the breeding for cotton varieties with the resistance to verticillium wilt has not been successful. hpa1xoo is a harpin protein from xanthomonas oryzae pv. oryzae which induces the hypersensitive cell death in plants. when hpa1xoo was transformed into the susceptible cotton line z3 ...201323143497
development of agrobacterium-mediated virus-induced gene silencing and performance evaluation of four marker genes in gossypium barbadense.gossypiumbarbadense is a cultivated cotton species and possesses many desirable traits, including high fiber quality and resistance to pathogens, especially verticilliumdahliae (a devastating pathogen of gossypium hirsutum, the main cultivated species). these elite traits are difficult to be introduced into g. hirsutum through classical breeding methods. in addition, genetic transformation of g. barbadense has not been successfully performed. it is therefore important to develop methods for eval ...201324023833
cotton ghmpk6a negatively regulates osmotic tolerance and bacterial infection in transgenic nicotiana benthamiana, and plays a pivotal role in development.mitogen-activated protein kinase (mapk) cascades play important roles in the perception of external signals and the generation of suitable responses. cotton (gossypium hirsutum) is an important fibre-producing and oil-producing crop worldwide. however, few mapks and their interaction partners have been functionally characterized in cotton. in the present study, the group a mapk g. hirsutum (gh)mpk6a was identified and characterized. ghmpk6a expression can be induced through multiple defence-rela ...201323957843
the effect of soil texture and irrigation on rotylenchulus reniformis and cotton.the reniform nematode, rotylenchulus reniformis, is the most damaging nematode pathogen of cotton in alabama. soil texture is currently being explored as a basis for the development of economic thresholds and management zones within a field. trials to determine the reproductive potential of r. reniformis as influenced by soil type were conducted in microplot and greenhouse settings during 2008 to 2010. population density of r. reniformis was significantly influenced by soil texture and exhibited ...201323833324
likelihood of stink bugs colonizing crops: a case study in southeastern farmscapes.stink bugs, including nezara viridula (l.), euschistus servus (say), and chinavia hilaris (say), are economic pests across agricultural farmscapes where they can colonize closely associated crops. this 4-yr on-farm study was conducted to examine the likelihood of these three stink bug species colonizing crops in corn-cotton, corn-peanut-cotton, and peanut-cotton farmscapes by using odds ratios. corn (zea mays l.), peanut (arachis hypogaea l.), and cotton (gossypium hirsutum l.) served as host pl ...201323726052
the cotton transcription factor tcp14 functions in auxin-mediated epidermal cell differentiation and elongation.plant-specific teosinte-branched1/cycloidea/pcf (tcp) transcription factors play crucial roles in development, but their functional mechanisms remain largely unknown. here, we characterized the cellular functions of the class i tcp transcription factor ghtcp14 from upland cotton (gossypium hirsutum). ghtcp14 is expressed predominantly in fiber cells, especially at the initiation and elongation stages of development, and its expression increased in response to exogenous auxin. induced heterologou ...201323715527
wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (gossypium hirsutum l.) tissues cultured in vitro.this work shows that overexpression of the wus gene from arabidopsis enhanced the expression of embryogenic competence and triggered organogenesis from some cells of the regenerated embryo-like structures. agrobacterium-mediated genetic transformation of cotton was described in the late 1980s, but is still time consuming and largely genotype dependant due to poor regeneration. to help solve this bottleneck, we over-expressed the wuschel (wus) gene, a homeobox transcription factor cloned in arabi ...201323543366
cloning and functional analysis of a novel ascorbate peroxidase (apx) gene from anthurium andraeanum.an 888-bp full-length ascorbate peroxidase (apx) complementary dna (cdna) gene was cloned from anthurium andraeanum, and designated as anapx. it contains a 110-bp 5'-noncoding region, a 28-bp 3'-noncoding region, and a 750-bp open reading frame (orf). this protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. the anapx protein showed 93%, 87%, 87%, 87%, and 86% similarities to the apx homologs from zantedeschia aethiopica, v ...201324302711
agroinoculation of a full-length cdna clone of cotton leafroll dwarf virus (clrdv) results in systemic infection in cotton and the model plant nicotiana benthamiana.cotton blue disease is the most important viral disease of cotton in the southern part of south america. its etiological agent, cotton leafroll dwarf virus (clrdv), is specifically transmitted to host plants by the aphid vector (aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. this limitation has held back the study of this virus and the disease it causes. in this study, a full-length cdna of clrdv was constructed and expressed in vivo un ...201323623981
development of insect-resistant transgenic cotton with chimeric tvip3a accumulating in chloroplasts.an optimized vip3a gene, designated as vip3a* was chemically synthesized and a thi1 gene chloroplast transit peptide coding sequence was attached to its 5' end to produce the tvip3a*. vip3a* and tvip3a* genes were transformed into gossypium hirsutum cv. zhongmiansuo35 mediated by agrobacterium tumefaciens. four independent transgenic t1 lines with single-copy insertions and unchanged phenotypes (ctv1 and ctv2 for tvip3a*, and cv1 and cv2 for vip3a*) were selected by polymerase chain reaction (pc ...201323143498
cotton ghmkk1 induces the tolerance of salt and drought stress, and mediates defence responses to pathogen infection in transgenic nicotiana benthamiana.mitogen-activated protein kinase kinases (mapkk) mediate a variety of stress responses in plants. so far little is known on the functional role of mapkks in cotton. in the present study, gossypium hirsutum mkk1 (ghmkk1) function was investigated. ghmkk1 protein may activate its specific targets in both the nucleus and cytoplasm. treatments with salt, drought, and h2o2 induced the expression of ghmkk1 and increased the activity of ghmkk1, while overexpression of ghmkk1 in nicotiana benthamiana en ...201323844212
construction of a plant-transformation-competent bibac library and genome sequence analysis of polyploid upland cotton (gossypium hirsutum l.).cotton, one of the world's leading crops, is important to the world's textile and energy industries, and is a model species for studies of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. here, we report the construction of a plant-transformation-competent binary bacterial artificial chromosome (bibac) library and comparative genome sequence analysis of polyploid upland cotton (gossypium hirsutum l.) with one of its diploid putative progenitor species, g. raimondii ulbr.201323537070
mosquito larvicidal potential of gossypium hirsutum (bt cotton) leaves extracts against aedes aegypti and anopheles stephensi larvae.we aimed to extract the ingredients from leaves of gossypium hirsutum (bt cotton) using different solvents and evaluate for potential use to control different larval stages of mosquito species, aedes aegypti and anopheles stephensi.201325629069
comparative transcriptome analysis of gossypium hirsutum l. in response to sap sucking insects: aphid and whitefly.cotton (gossypium hirsutum l.) is a major fiber crop that is grown worldwide; it faces extensive damage from sap-sucking insects, including aphids and whiteflies. genome-wide transcriptome analysis was performed to understand the molecular details of interaction between gossypium hirsutum l. and sap-sucking pests, namely aphis gossypii (aphid) and bemisia tabacci (whiteflies). roche's gs-titanium was used to sequence transcriptomes of cotton infested with aphids and whiteflies for 2 h and 24 h.201323577705
coordinate accumulation of homeologous transcripts of seven cotton lea gene families during embryogenesis and germination.one of two related patterns of total transcript accumulation are seen during embryogenesis for 18 cotton lea (late embryogenesis-abundant) gene families in the allotetraploid cotton gossypium hirsutum l. cv coker 201. coordinate accumulation in each class is complex, suggesting that lea mrna abundance is regulated by several events. each of the lea gene families probably contains two active homeologous genes (alloalleles), one in each of cotton's two subgenomes. it is of interest whether both tr ...20133622929
a transcript profiling approach reveals an abscisic acid-specific glycosyltransferase (ugt73c14) induced in developing fiber of ligon lintless-2 mutant of cotton (gossypium hirsutum l.).ligon lintless-2, a monogenic dominant cotton (gossypium hirsutum l.) fiber mutation, causing extreme reduction in lint fiber length with no pleiotropic effects on vegetative growth, represents an excellent model system to study fiber elongation. a udp-glycosyltransferase that was highly expressed in developing fibers of the mutant ligon lintless-2 was isolated. the predicted amino acid sequence showed ~53% similarity with arabidopsis ugt73c sub-family members and the udp-glycosyltransferase was ...201324086489
spatial and temporal variation in fungal endophyte communities isolated from cultivated cotton (gossypium hirsutum).studies of fungi in upland cotton (gossypium hirsutum) cultivated in the united states have largely focused on monitoring and controlling plant pathogens. given increasing interest in asymptomatic fungal endophytes as potential biological control agents, surveys are needed to better characterize their diversity, distribution patterns and possible applications in integrated pest management. we sampled multiple varieties of cotton in texas, usa and tested for temporal and spatial variation in fung ...201323776604
overexpression of ft in cotton affects architecture but not floral organogenesis.flowering marks the change from indeterminate to determinate plant growth, and this developmental transition involves the activity of the arabidopsis flowering locus t (ft) gene product and its orthologs. we demonstrated that when ft is ectopically expressed from a viral vector in cotton, a process referred to as virus induced flowering (vif), it uncouples flowering from photoperiodic regulation and promotes determinate growth in aerial organs. the accelerated switch to determinate growth affect ...201323333977
the relationship between environmental variables and response of cotton to nematicides.nematicide/irrigation rate trials were conducted in texas (tx) in 2009 and 2010 in cotton grown at three irrigation rates, where irrigation rate (base (b), b - 33%, b + 33%) was the main plot and treatment (untreated check, aldicarb, and nematicide seed treatment (nst) and nst + aldicarb) were the subplots. aldicarb improved cotton lint yield with the base (medium) irrigation rate over the untreated check, but not at the b - 33% and b + 33% irrigation rates. in a second evaluation, 20 tests cond ...201323589654
analyzing blends of herbivore-induced volatile organic compounds with factor analysis: revisiting "cotton plant, gossypium hirsutum l., defense in response to nitrogen fertilization".many herbivorous, predaceous, and parasitic insects use constitutive and herbivore-induced volatile organic compounds (vocs) to locate their respective host plant, prey, and hosts. multivariate statistical tools (e.g., factor analysis) are recognized increasingly as an appropriate approach for analyzing intercorrelated data such as presence/absence or quantities of vocs. one challenge of implementing factor analysis is determining how many new variables (factors) to retain in the final analysis. ...201323786100
preference of a polyphagous mirid bug, apolygus lucorum (meyer-dür) for flowering host plants.apolygus lucorum (meyer-dür) (hemiptera: miridae) is one of the most important herbivores in a broad range of cultivated plants, including cotton, cereals, vegetables, and fruit crops in china. in this manuscript, we report on a 6-year long study in which (adult) a. lucorum abundance was recorded on 174 plant species from 39 families from early july to mid-september. through the study period per year, the proportion of flowering plants exploited by adult a. lucorum was significantly greater than ...201323874835
synthesis of resveratrol glycosides by cultured plant cells.incubation of cultured cells of glycine max with trans-resveratrol gave its 3-o-beta-d- and 4'-o-beta-d-glucosides. cultured gossypium hirsutum cells glycosylated trans-resveratrol to its 3-o-beta-d-, 4'-o-beta-d-, and 3,4'-o-beta-d-diglucosides. on the other hand, trans-resveratrol was converted into cis-resveratrol 4'-o-beta-d-glucoside, together with trans-resveratrol 3-o-beta-d-glucoside and trans-resveratrol 4'-o-beta-d-glucoside, by eucalyptus perriniana.201323980421
cysteine protease enhances plant-mediated bollworm rna interference.oral ingestion of plant-expressed double stranded rna (dsrna) triggers target gene suppression in insect. an important step of this process is the transmission of dsrna from plant to midgut cells. insect peritrophic matrix (pm) presents a barrier that prevents large molecules from entering midgut cells. here, we show that uptake of plant cysteine proteases, such as ghcp1 from cotton (gossypium hirsutum) and atcp2 from arabidopsis, by cotton bollworm (helicoverpa armigera) larvae resulted in atte ...201323460027
insights into the evolution of cotton diploids and polyploids from whole-genome re-sequencing.understanding the composition, evolution, and function of the gossypium hirsutum (cotton) genome is complicated by the joint presence of two genomes in its nucleus (at and dt genomes). these two genomes were derived from progenitor a-genome and d-genome diploids involved in ancestral allopolyploidization. to better understand the allopolyploid genome, we re-sequenced the genomes of extant diploid relatives that contain the a1 (gossypium herbaceum), a2 (gossypium arboreum), or d5 (gossypium raimo ...201323979935
mapping quantitative trait loci for lint yield and fiber quality across environments in a gossypium hirsutum × gossypium barbadense backcross inbred line population.identification of stable quantitative trait loci (qtls) across different environments and mapping populations is a prerequisite for marker-assisted selection (mas) for cotton yield and fiber quality. to construct a genetic linkage map and to identify qtls for fiber quality and yield traits, a backcross inbred line (bil) population of 146 lines was developed from a cross between upland cotton (gossypium hirsutum) and egyptian cotton (gossypium barbadense) through two generations of backcrossing u ...201323064252
interspecific chromosomal effects on agronomic traits in gossypium hirsutum by ad analysis using intermated g. barbadense chromosome substitution lines.the untapped potential of the beneficial alleles from gossypium barbadense l. has not been well utilized in g. hirsutum l. (often referred to as upland cotton) breeding programs. this is primarily due to genomic incompatibility and technical challenges associated with conventional methods of interspecific introgression. in this study, we used a hypoaneuploid-based chromosome substitution line as a means for systematically introgressing g. barbadense doubled-haploid line '3-79' germplasm into a c ...201322945267
modulation of reproductive behaviors by non-host volatiles in the polyphagous egyptian cotton leafworm, spodoptera littoralis.in order to locate mates, food, and oviposition sites, insects mainly rely on volatile cues released by their sexual partners, food sources, and host and non-host plants. calling, mating, and oviposition behaviors, as well as fecundity and longevity, of newly emerged spodoptera littoralis (bois.) moths were recorded in the presence of volatiles from leaves of a host plant, gossypium hirsutum (cotton) and two non-host plants, adhatoda vasica (av) or picea abies (spruce), either alone or in host/n ...201324105603
functional analysis of the seed coat-specific gene gbmyb2 from cotton.myb transcription factors are essential for cotton fiber development. we isolated the r2r3-myb gene gbmyb2 from gossypium barbadense. rna in situ hybridization analysis showed that gbmyb2 is mainly expressed in the outer integuments of cotton ovules and in elongating fibers. gbmyb2 expression increased throughout fiber initiation and during the elongation stage. the expression level of gbmyb2 was higher in the gossypium hirsutum cultivar xu142 than in the xu142 (fl) mutant. overexpression of gbm ...201324036393
specific response to herbivore-induced de novo synthesized plant volatiles provides reliable information for host plant selection in a moth.animals depend on reliable sensory information for accurate behavioural decisions. for herbivorous insects it is crucial to find host plants for feeding and reproduction, and these insects must be able to differentiate suitable from unsuitable plants. volatiles are important cues for insect herbivores to assess host plant quality. it has previously been shown that female moths of the egyptian cotton leafworm, spodoptera littoralis (lepidoptera: noctuidae), avoid oviposition on damaged cotton gos ...201323737555
a genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin.the cotton (gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. by mining public data and comparing analyses of fiber from two cotton species (gossypium hirsutum and gossypium barbadense), we found that th ...201323535943
an integrative analysis of four cesa isoforms specific for fiber cellulose production between gossypium hirsutum and gossypium barbadense.cotton fiber is an excellent model system of cellulose biosynthesis; however, it has not been widely studied due to the lack of information about the cellulose synthase (cesa) family of genes in cotton. in this study, we initially identified six full-length cesa genes designated as ghcesa5-ghcesa10. phylogenetic analysis and gene co-expression profiling revealed that cesa1, cesa2, cesa7, and cesa8 were the major isoforms for secondary cell wall biosynthesis, whereas cesa3, cesa5, cesa6, cesa9, a ...201323508664
cotton fiber cell walls of gossypium hirsutum and gossypium barbadense have differences related to loosely-bound xyloglucan.cotton fiber is an important natural textile fiber due to its exceptional length and thickness. these properties arise largely through primary and secondary cell wall synthesis. the cotton fiber of commerce is a cellulosic secondary wall surrounded by a thin cuticulated primary wall, but there were only sparse details available about the polysaccharides in the fiber cell wall of any cotton species. in addition, gossypium hirsutum (gh) fiber was known to have an adhesive cotton fiber middle lamel ...201323457548
efficient decolorization and deproteinization using uniform polymer microspheres in the succinic acid biorefinery from bio-waste cotton (gossypium hirsutum l.) stalks.bio-waste cotton (gossypium hirsutum l.) stalks were converted into succinic acid by simultaneous saccharification and fermentation (ssf) using actinobacillus succinogenes 130z. after 54 h ssf at 40 °c and ph 7.0, the production of succinic acid was 63 g/l, with 1.17 g/l/h productivity and 64% conversion yield. after ssf, a simple method for the decolorization and deproteinization of crude ssf broth was developed through adsorption tests of polystyrene (pst) microspheres. under optimized conditi ...201322985822
high-frequency regeneration via multiple shoot induction of an elite recalcitrant cotton (gossypium hirsutum l. cv narashima) by using embryo apex.cotton (gossypium hirsutum l.) is one of the most commercially important fiber crops in the world. compared with other crops, cotton represents a recalcitrant species for regeneration protocols. the development of efficient and rapid regeneration protocol for elite indian cotton variety could help improve the quality characteristics and biotic or abiotic stress tolerance. here we report a novel regeneration protocol in indian cotton cultivar narashima. the maximum number of multiple shoots obtai ...201323221745
a fasciclin-like arabinogalactan protein, ghfla1, is involved in fiber initiation and elongation of cotton.arabinogalactan proteins (agps) are involved in many aspects of plant development. in this study, biochemical and genetic approaches demonstrated that agps are abundant in developing fibers and may be involved in fiber initiation and elongation. to further investigate the role of agps during fiber development, a fasciclin-like arabinogalactan protein gene (ghfla1) was identified in cotton (gossypium hirsutum). overexpression of ghfla1 in cotton promoted fiber elongation, leading to an increase i ...201323349362
synergistic effects of ghsod1 and ghcat1 overexpression in cotton chloroplasts on enhancing tolerance to methyl viologen and salt stresses.in plants, cuzn superoxide dismutase (cuznsod, ec l.15.1.1), ascorbate peroxidase (apx, ec 1.11.1.11), and catalase (cat, ec l.11.1.6) are important scavengers of reactive oxygen species (ros) to protect the cell from damage. in the present study, we isolated three homologous genes (ghsod1, ghapx1, and ghcat1) from gossypium hirsutum. overexpressing cassettes containing chimeric ghsod1, ghapx1, or ghcat1 were introduced into cotton plants by agrobacterium transformation, and overexpressed produc ...201323335985
elucidation of nuclear and organellar genomes of gossypium hirsutum: furthering studies of species evolution and applications for crop improvement.plant genomes are larger and more complex than other eukaryotic organisms, due to small and large duplication events, recombination and subsequent reorganization of the genetic material. commercially important cotton is the result of a polyploidization event between old and new world cottons that occurred over one million years ago. allotetraploid cotton has properties that are dramatically different from its progenitors-most notably, the presence of long, spinnable fibers. recently, the complet ...201324833222
field-acclimated gossypium hirsutum cultivars exhibit genotypic and seasonal differences in photosystem ii thermostability.previous investigations have demonstrated that photosystem ii (psii) thermostability acclimates to prior exposure to heat and drought, but contrasting results have been reported for cotton (gossypium hirsutum). we hypothesized that psii thermotolerance in g. hirsutum would acclimate to environmental conditions during the growing season and that there would be differences in psii thermotolerance between commercially-available u.s. cultivars. to this end, three cotton cultivars were grown under dr ...201323246028
agrobacterium-mediated transformation of cotton (gossypium hirsutum) shoot apex with a fungal phytase gene improves phosphorus acquisition.cotton is an important world economic crop plant. it is considered that cotton is recalcitrant to in vitro proliferation. somatic embryogenesis and plant regeneration has been successful by using hypocotyl, whereas it is highly genotype dependent. here, a genotype-independent cotton regeneration protocol from shoot apices is presented. shoot apices from 3- to 5-day-old seedlings of cotton are infected with an agrobacterium strain, eha105, carrying the binary vector pc-ksa contained phytase gene ...201323143496
investigating transgene integration and organization in cotton (gossypium hirsutum l.) genome.in this chapter, we present detailed experimental procedures for investigating integration patterns of transgenes in cotton genome. we use conventional pcr and genomic southern blot hybridization to characterize integration of t-dna components and vector backbone fragments. for multiple copy insertions into the same site (complex loci), transgene/transgene junctions (including canonical and truncated t-dna and transgene involved vector backbone sequences) are characterized by pcr and sequencing. ...201323143486
silicon carbide whisker-mediated transformation of cotton (gossypium hirsutum l.).plant transformation methods are invaluable biotechnological tools to generate specific and targeted genetic variation for performance improvement of crop plants. genetic information is created by proper modification during gene cloning flanked by proper regulatory sequences and delivered to plants via -different plant transformation techniques. due to being a multipurpose plant, cotton has been subjected to different genetic transformation methods to provide the breeders with an opportunity to ...201323143485
biolistic transformation of cotton embryogenic cell suspension cultures.genetic transformation of cotton (gossypium hirsutum l.) is highly dependent on the ability to regenerate fertile plants from transgenic cells through somatic embryogenesis. induction of embryogenic cell cultures is genotype dependant. however, once embryogenic cell cultures are available, they can be effectively used for transformation by agrobacterium or biolistic bombardment methods. here i describe a detailed procedure to transform cotton embryogenic cell suspension cultures by biolistic bom ...201323143483
biolistic transformation of cotton zygotic embryo meristem.biolistic transformation of cotton (gossypium hirsutum l.) meristems, isolated from mature seed, is detailed in this report. a commercially available, helium-driven biolistic device (bio-rad pds1000/he) was used to bombard gold particles coated with a marker gene (uida or "β-glucuronidase") into the shoot meristem. the penetration of gold particles was dependent on bombardment parameters and it was mostly one to two cell layers deep. stable transformation of epidermal l1 layer was consistently o ...201323143482
rnai-mediated ultra-low gossypol cottonseed trait: performance of transgenic lines under field conditions.cottonseed remains a low-value by-product of lint production mainly due to the presence of toxic gossypol that makes it unfit for monogastrics. ultra-low gossypol cottonseed (ulgcs) lines were developed using rnai knockdown of δ-cadinene synthase gene(s) in gossypium hirsutum. the purpose of the current study was to assess the stability and specificity of the ulgcs trait and evaluate the agronomic performance of the transgenic lines. trials conducted over a period of 3 years show that the ulgcs ...201323078138
extraction and characterization of nanocellulose structures from raw cotton linter.this study aimed to characterize nanocellulose extracted from cotton (gossypium hirsutum) linters. the nanocellulose was subjected to electronic microscopy, thermal analysis, x-ray diffractometry, light scattering, and contact angle. the properties of the nanocellulose are considerably different from the linter. the acidic hydrolyses applied to extract the nanocrystals increased the crystallinity index and the hydrophilicity and decreased the thermal stability. on average, the nanocrystals were ...201323044127
construction of microsatellite-based linkage map and mapping of nectarilessness and hairiness genes in gossypium tomentosum.gossypium tomentosum, a wild tetraploid cotton species with ad genomes, possesses genes conferring strong fibers and high heat tolerance. to effectively transfer these genes into gossypium hirsutum, an entire microsatellite (simple sequence repeat, ssr)-based genetic map was constructed using the interspecific cross of g. hirsutum x g. tomentosum (ht). we detected 1800 loci from 1347 pairs of polymorphic primers. of these, 1204 loci were grouped into 35 linkage groups at lod ≥ 4. the map covers ...201324371166
genome-wide functional analysis of cotton (gossypium hirsutum) in response to drought.cotton is one of the most important crops for its natural textile fibers in the world. however, it often suffered from drought stress during its growth and development, resulting in a drastic reduction in cotton productivity. therefore, study on molecular mechanism of cotton drought-tolerance is very important for increasing cotton production. to investigate molecular mechanism of cotton drought-resistance, we employed rna-seq technology to identify differentially expressed genes in the leaves o ...201324260499
transcriptome analysis reveals crosstalk of responsive genes to multiple abiotic stresses in cotton (gossypium hirsutum l.).abiotic stress is a major environmental factor that limits cotton growth and yield, moreover, this problem has become more and more serious recently, as multiple stresses often occur simultaneously due to the global climate change and environmental pollution. in this study, we sought to identify genes involved in diverse stresses including abscisic acid (aba), cold, drought, salinity and alkalinity by comparative microarray analysis. our result showed that 5790, 3067, 5608, 778 and 6148 transcri ...201324224045
the dual functions of wlim1a in cell elongation and secondary wall formation in developing cotton fibers.lin-11, isl1 and mec-3 (lim)-domain proteins play pivotal roles in a variety of cellular processes in animals, but plant lim functions remain largely unexplored. here, we demonstrate dual roles of the wlim1a gene in fiber development in upland cotton (gossypium hirsutum). wlim1a is preferentially expressed during the elongation and secondary wall synthesis stages in developing fibers. overexpression of wlim1a in cotton led to significant changes in fiber length and secondary wall structure. comp ...201324220634
the promoter structure differentiation of a myb transcription factor rlc1 causes red leaf coloration in empire red leaf cotton under light.the red leaf coloration of empire red leaf cotton (erlc) (gossypium hirsutum l.), resulted from anthocyanin accumulation in light, is a well known dominant agricultural trait. however, the underpin molecular mechanism remains elusive. to explore this, we compared the molecular biological basis of anthocyanin accumulation in both erlc and the green leaf cotton variety ccri 24 (gossypium hirsutum l.). introduction of r2r3-myb transcription factor rosea1, the master regulator anthocyanin biosynthes ...201324205014
identification and characterization of (e)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton.cotton (gossypium hirsutum l.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. to understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, ghtps1 and ghtps2, were heterologously expressed and characterized. recombinant ghtps1 accepted farnesyl pyrophosphate as substrate and prod ...201324184450
generation and analysis of a large-scale expressed sequence tag database from a full-length enriched cdna library of developing leaves of gossypium hirsutum l.cotton (gossypium hirsutum l.) is one of the world's most economically-important crops. however, its entire genome has not been sequenced, and limited resources are available in genbank for understanding the molecular mechanisms underlying leaf development and senescence.201324146870
linkage mapping and expression analysis of mirnas and their target genes during fiber development in cotton.micrornas (mirnas) are small, endogenously expressed, non-coding rna molecules involved in gene transcription and expression that combine with specific mrna site of target genes to inhibit protein synthesis or degrade mrna. since the first plant mirna was reported in 2002, numerous new mirnas and their targets have been discovered via high-throughput sequencing and computational approaches. however, the genetic variation of mirna genes is poorly understood due to the lack of mirna-specific dna m ...201324131852
bac-pool sequencing and analysis of large segments of a12 and d12 homoeologous chromosomes in upland cotton.although new and emerging next-generation sequencing (ngs) technologies have reduced sequencing costs significantly, much work remains to implement them for de novo sequencing of complex and highly repetitive genomes such as the tetraploid genome of upland cotton (gossypium hirsutum l.). herein we report the results from implementing a novel, hybrid sanger/454-based bac-pool sequencing strategy using minimum tiling path (mtp) bacs from ctg-3301 and ctg-465, two large genomic segments in a12 and ...201324116150
isolation and characterization of terpene synthases in cotton (gossypium hirsutum).cotton plants accumulate gossypol and related sesquiterpene aldehydes, which function as phytoalexins against pathogens and feeding deterrents to herbivorous insects. however, to date little is known about the biosynthesis of volatile terpenes in this crop. herein is reported that 5 monoterpenes and 11 sesquiterpenes from extracts of a glanded cotton cultivar, gossypium hirsutum cv. ccri12, were detected by gas chromatography-mass spectrometry (gc-ms). by est data mining combined with rapid ampl ...201324074555
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