Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| increase in desulfurization activity of rhodococcus erythropolis ka2-5-1 using ethanol feeding. | rhodococcus erythropolis ka2-5-1 is one of the best strains for the desulfurization of dibenzothiophene (dbt) via a sulfur-specific pathway in which dbt is converted to the end product, 2-hydroxybiphenyl, by releasing sulfite via dbt-sulfone and 2-(2'-hydroxyphenyl) benzene sulphinate. the objective of this research is to develop a culture method in order to attain a high cell density with a high level of specific desulfurization activity. compared with glucose or glycerol, ethanol was found to ... | 2000 | 16232759 |
| production of cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) by rhodococcus sp. ma 7249. | an attractive method for producing cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) was developed employing a cis dihydrodiol dehydrogenase "deficient" strain of rhodococcus (ma 7249). the toluene cis glycol produced was found to have optical rotations of [alpha]d25 = +25.8 (c 0.45, ch3oh) and +72.8 (c 0.42, chcl3) which indicated an absolute configuration of (1s,2r) when compared with previously published values. when cultivated in laboratory fermentor in the presence of tolue ... | 2000 | 16232863 |
| consecutive bacillary angiomatosis and rhodococcus equi bacteremia during acute leukemia: zoonoses may cause fever in neutropenic patients. | 2000 | 11168145 | |
| pulmonary infection caused by rhodococcus equi in hiv-infected patients: report of four patients from northern thailand. | we report four human immunodeficiency virus (hiv)-infected patients (3 men and one woman, average age, 34.3 years) with pulmonary infection (two with pneumonia and two with lung abscess) caused by rhodococcus equi. these patients, who presented with fever and productive cough, were admitted to nakornping hospital in northern thailand. chest roentgenograms showed pulmonary infiltration and/or cavitary lesions. their conditions were poor because of severe anemia, and transfusion was necessary in t ... | 2000 | 11810571 |
| resident aerobic microbiota of the adult human nasal cavity. | recent evidence strongly suggests that the microbiota of the nasal cavity plays a crucial role in determining the reaction patterns of the mucosal and systemic immune system. however, little is known about the normal microbiota of the nasal cavity. the purpose of this study was to determine the microbiota in different parts of the nasal cavity and to develop and evaluate methods for this purpose. samples were collected from 10 healthy adults by nasal washes and by swabbing of the mucosa through ... | 2000 | 11200821 |
| the role of apoptosis in normal ontogenesis and solid human neoplasms. | mammalian cells are capable of committing "active suicide" or apoptosis in response to specialized pathological mechanisms employing a phylogenetically developed intrinsic program of death, triggered by signal transduction through specific receptors. changes in cellular structure such as: 1) condensation of the nuclear (chromatin) and cytoplasmic structures (especially the mitochondria); 2) blebbing of the cell membrane; 3) characteristic swelling of the endoplasmic reticulum; and 4) fragmentati ... | 2000 | 11204498 |
| enhancing desulphurization by engineering a flavin reductase-encoding gene cassette in recombinant biocatalysts. | biological desulphurization of petroleum feedstocks and products may offer an attractive alternative to reduce sulphur oxide emissions that cause serious environmental pollution. dibenzothiophene (dbt) desulphurization via the dsz pathway of rhodococcus erythropolis igts8 is an energetically expensive process that consumes reducing equivalents. we have shown in this work that the hpac oxidoreductase from escherichia coli w is able to supply the required fmnh2 to the dsz monooxygenases. the cloni ... | 2000 | 11214801 |
| bacterial sources and sinks of isoprene, a reactive atmospheric hydrocarbon. | 2000 | 11220299 | |
| [clusterization of halophilic and halotolerant eubacteria using whole-cell protein electrophoresis data]. | total cell proteins of the nineteen halophilic and halotolerant eubacteria isolated from marine sediments and highly mineralized formation waters of oil fields were investigated by sds gel electrophoresis. the microorganisms studied, phenotypically identified as belonging to the genera dietzia, rhodococcus, staphylococcus, cytophaga, brevibacterium, and archangium, were found to form clearly distinguishable clusters (20-30% similarity at the generic level) on the dendrogram derived from electrop ... | 2000 | 11315673 |
| [cavitating pneumonia in a patient infected with human immunodeficiency virus]. | 2000 | 11075487 | |
| dna sequence and comparison of virulence plasmids from rhodococcus equi atcc 33701 and 103. | the virulence plasmids of the equine virulent strains rhodococcus equi atcc 33701 and 103 were sequenced, and their genetic structure was analyzed. p33701 was 80,610 bp in length, and p103 was 1 bp shorter; their sequences were virtually identical. the plasmids contained 64 open reading frames (orfs), 22 of which were homologous with genes of known function and 3 of which were homologous with putative genes of unknown function in other species. putative functions were assigned to five orfs based ... | 2000 | 11083803 |
| pharmacokinetics of erythromycin estolate and erythromycin phosphate after intragastric administration to healthy foals. | to determine pharmacokinetics and plasma concentrations of erythromycin and related compounds after intragastric administration of erythromycin phosphate and erythromycin estolate to healthy foals. | 2000 | 10951982 |
| application of solid-phase extraction to the analysis of the isomers generated in biodesulfurization against methylated dibenzothiophenes. | a solid-phase extraction (spe) technique was applied to analyze and characterize the biodesulfurization reactions against asymmetrically methylated dibenzothiophenes (mdbts) such as 1-, 2-, 3- and 4-methyldibenzothiophenes present in fossil fuels. recently, we found that these mdbts are efficiently degraded by the bacterial strain, rhodococcus erythropolis ka2-5-1. separation and concentration of the microbial desulfurization products from each of the mdbts could be carried out with high efficie ... | 2000 | 11153941 |
| rhodococcus pyridinivorans sp. nov., a pyridine-degrading bacterium. | the taxonomic position of a bacterial strain (pdb9t) that is capable of degrading pyridine was clarified by a polyphasic taxonomic approach using phenotypic, chemotaxonomic and genetic methods. the cells, which are rods and branched filaments during the early growth phase, fragment into short rods or cocci, thereby completing the growth cycle. strain pdb9t was found to have a cell wall of chemotype iv, mk-8(h2) as the predominant menaquinone, mycolic acids with 36-46 carbon atoms and c16:0' c18: ... | 2000 | 11155994 |
| crystal structure of the haloalkane dehalogenase from sphingomonas paucimobilis ut26. | the haloalkane dehalogenase from sphingomonas paucimobilis ut26 (linb) is the enzyme involved in the degradation of the important environmental pollutant gamma-hexachlorocyclohexane. the enzyme hydrolyzes a broad range of halogenated cyclic and aliphatic compounds. here, we present the 1.58 a crystal structure of linb and the 2.0 a structure of linb with 1,3-propanediol, a product of debromination of 1,3-dibromopropane, in the active site of the enzyme. the enzyme belongs to the alpha/beta hydro ... | 2000 | 11087355 |
| evaluation of nasotracheal aspiration as a diagnostic tool for rhodococcus equi pneumonia in foals. | the reliability of preparing bacteriological cultures from nasotracheal aspirates of foals routinely in order to diagnose r. equi pneumonia in foals was studied by isolating rhodococcus equi from specimens obtained from 96 foals by nasotracheal aspiration with a silicon catheter. results were compared with specimens obtained from 21 foals by transtracheal aspiration (percutaneous tracheal puncture). these 117 foals showed clinical signs of respiratory tract infection at sampling. r. equi was iso ... | 2000 | 11093633 |
| composition of soil microbial communities enriched on a mixture of aromatic hydrocarbons. | soil contaminated with c5+, which contained benzene (45%, wt/wt), dicyclopentadiene (dcpd) plus cyclopentadiene (together 20%), toluene (6%), styrene (3%), xylenes (2%), naphthalene (2%), and smaller quantities of other compounds, served as the source for isolation of 55 genomically distinct bacteria (standards). use of benzene as a substrate by these bacteria was most widespread (31 of 44 standards tested), followed by toluene (23 of 44), xylenes (14 of 44), styrene (10 of 44), and naphthalene ... | 2000 | 11097903 |
| isolation and characterization of corynebacterium ulcerans from cephalic implants in macaques. | to determine the prevalence of colonization by corynebacterium ulcerans, we cultured samples from the cephalic implant-skin margin and pharynx of 26 rhesus macaques and one pig-tailed macaque. all but one of the samples from the cephalic implants yielded a mixed population of bacteria. c. ulcerans grew from the cephalic implants in 56% and from the pharynx in 3% of the implanted animals. we screened nine of these isolates for diphtheria toxin (dt) and phospholipase d (pld). polymerase chain reac ... | 2000 | 11099137 |
| biodesulfurization and the upgrading of petroleum distillates. | biotechnology offers an alternative way to process fossil fuels. there have been several important advances in the elucidation of the mechanisms of biodesulfurization and the development of a biocatalytic desulfurization process. these include a detailed analysis of the rate and extent of desulfurization of real target molecules in a diesel matrix, the directed evolution of rate- and extent-limiting enzymes for better performance and the expression of the genes in alternative hosts. process inno ... | 2000 | 11102787 |
| purification, characterization and crystallization of enzymes for dibenzothiophene desulfurization. | dszc and dsza, dbt monooxygenase and dbt sulfone monooxygenase, respectively, involved in dibenzothiophene (dbt) desulfurization, were purified to homogeneity from rhodococcus erythropolis d-1. the two enzymes were crystallized and enzymologically characterized. we found a high activity of flavin reductase in the non-dbt-desulfurizing bacterium, paenibacillus polymyxa a-1, which is essential for dszc and a activities, and purified to homogeneity and characterized the enzyme. | 2000 | 11105249 |
| [study of the integral toxicity of aqueous media, polluted by petroleum and petroleum products using bacterial tests]. | a biotest kit was used to assess the integral toxicity level of aquatic medium contamination with petroleum and petroleum-based products. the integral toxicity dynamics was also monitored during biodegradation of petroleum and petroleum-based products by an association of petroleum-degrading strains including acinetobacter sp., mycobacterium flavescens, and rhodococcus sp. the following bacterial tests were used: the bioluminescence (bl) test based on photobacterium leiognathi; electro-orientati ... | 2000 | 11116909 |
| [creation and use of a liquid substance based on association of petroleum-oxidizing bacteria]. | an association of four bacterial strains with high oil-oxidizing and bioemulsifying activities, psychrophilicity, resistance to chemical pollutants, and lack of pathogenicity was selected from a collection of natural oil-oxidizing microorganisms. a new liquid preparation containing stabilizers and preservatives that maintain the cell viability and oil-oxidizing activity during long-term storage was developed. a field experiment in oil-polluted sod-podzol and clay sand soils demonstrated that thi ... | 2000 | 11116911 |
| rhodococcus equi and hiv-1 infection in uganda. | to describe three cases of rhodococcus equi infection in a cohort of hiv-1 infected adults in entebbe, uganda and to compare this to the rates and presentation of tuberculosis in this cohort. | 2000 | 11120609 |
| salt-tolerant phenol-degrading microorganisms isolated from amazonian soil samples. | two phenol-degrading microorganisms were isolated from amazonian rain forest soil samples after enrichment in the presence of phenol and a high salt concentration. the yeast candida tropicalis and the bacterium alcaligenes faecoalis were identified using several techniques, including staining, morphological observation and biochemical tests, fatty acid profiles and 16s/18s rrna sequencing. both isolates, a. faecalis and c. tropicalis, were used in phenol degradation assays, with rhodococcus eryt ... | 2000 | 11131025 |
| characterization of the gene cluster involved in isoprene metabolism in rhodococcus sp. strain ad45. | the genes involved in isoprene (2-methyl-1,3-butadiene) utilization in rhodococcus sp. strain ad45 were cloned and characterized. sequence analysis of an 8.5-kb dna fragment showed the presence of 10 genes of which 2 encoded enzymes which were previously found to be involved in isoprene degradation: a glutathione s-transferase with activity towards 1,2-epoxy-2-methyl-3-butene (isoi) and a 1-hydroxy-2-glutathionyl-2-methyl-3-butene dehydrogenase (isoh). furthermore, a gene encoding a second gluta ... | 2000 | 10715003 |
| trehalose 6,6'-dimycolate (cord factor) enhances neovascularization through vascular endothelial growth factor production by neutrophils and macrophages. | trehalose 6,6'-dimycolate (tdm) plays important roles in the development of granulomatous inflammation during infection with mycobacterium spp., rhodococcus spp., etc. to reveal the augmenting effect of tdm on vascular endothelial growth factor (vegf) production and neovascularization, we investigated murine granulomatous tissue air pouches induced by rhodococcus sp. strain 4306 tdm dissolved in freund's incomplete adjuvant (fia), comparing them to pouches treated with fia alone. histologically, ... | 2000 | 10722600 |
| gene cloning and nucleotide sequencing and properties of a cocaine esterase from rhodococcus sp. strain mb1. | a strain of rhodococcus designated mb1, which was capable of utilizing cocaine as a sole source of carbon and nitrogen for growth, was isolated from rhizosphere soil of the tropane alkaloid-producing plant erythroxylum coca. a cocaine esterase was found to initiate degradation of cocaine, which was hydrolyzed to ecgonine methyl ester and benzoate; both of these esterolytic products were further metabolized by rhodococcus sp. strain mb1. the structural gene encoding a cocaine esterase, designated ... | 2000 | 10698749 |
| evaluation of mini-vidas rapid test for detection of listeria monocytogenes from production lines of fresh to cold-smoked fish. | this study was conducted to evaluate the efficacy of the mini-vidas listeria monocytogenes (lmo) system (biomérieux vitek, inc., missouri, usa) for detection of l. monocytogenes in environmental and fish samples from three portuguese cold-smoking plants and from their fresh fish suppliers. mini-vidas-lmo is a fully automated system that uses fluorescent elfa (enzyme linked fluorescent assay) technology for detection of listeria monocytogenes antigens in food. it can be a rapid screening method a ... | 2000 | 10699670 |
| [rhodococcus equi brain abscess in a patient in hemodialysis]. | 2000 | 11039267 | |
| [a diagnostic medium for arcanobacterium haemolyticum and other bacterial species reacting with hemolytic synergism to the equi-factor of rhodococcus equi]. | colonies of arcanobacterium haemolyticum on common blood agar can be easily overlooked. therefore a diagnostic medium was developed, on which a. haemolyticum colonies produce a conspicuous zone of complete hemolysis. the medium under question is blood agar prepared from the columbia blood agar base and 5% washed sheep erythrocytes sensitised with equi factor (ef) of rhodococcus equi. optimally, 10 activity units (au) of ef per 1 ml were used. ef was titrated on a non-nutrient medium consisting o ... | 2000 | 11040494 |
| identification of corynebacterium bovis and other coryneforms isolated from bovine mammary glands. | bovine mastitis remains the most economically important disease in dairy cows. corynebacterium bovis, a lipid-requiring corynebacterium spp., is frequently isolated from the milk of infected mammary glands of dairy cows and is associated with reduced milk production. a total of 212 coryneform bacteria isolated from the milk of dairy cows were obtained from mastitis reference laboratories in the united states and canada. all isolates had been presumptively identified as corynebacterium bovis base ... | 2000 | 11049082 |
| relationships between colony morphotypes and oil tolerance in rhodococcus rhodochrous. | a mucoidal strain of rhodococcus rhodochrous was resistant to 10% (vol/vol) n-hexadecane, while its rough derivatives were sensitive. when the extracellular polysaccharide (eps) produced by the mucoidal strain was added to cultures of the rough strains, the rough strains gained resistance to n-hexadecane. thus, eps confer tolerance to n-hexadecane in members of the genus rhodococcus. | 2000 | 11055965 |
| [rhodococcus equi pneumonia in patients with aids]. | rhodococus equi infection is frequent in animals, but rare in humans. it usually appears as an opportunistic infection, occurring in clinical settings where a compromise of cellular immunity exists, such as in leukemia, neoplastic or transplanted patients. it is currently considered, as another opportunistic aids infection. sixty six cases of rhodococus equi pneumonia have been published until march 1996. the differential diagnosis includes tb and staphyloccocal pneumonia and is generally an inf ... | 2000 | 11059055 |
| clinical application of a polymerase chain reaction assay in the diagnosis of pneumonia caused by rhodococcus equi in a horse. | diagnosis of pneumonia caused by rhodococcus equi can be made more rapidly by use of a polymerase chain reaction (pcr) assay than by use of conventional bacteriologic culture techniques. use of a pcr assay aids in the differentiation between virulent and avirulent strains of r equi, and the assay may be used to identify r equi in feces and soil of breeding farms. | 2000 | 11061388 |
| cloning and expression of gene encoding a novel endoglycoceramidase of rhodococcus sp. strain c9. | endoglycoceramidase (egcase) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. we previously reported that the asn-glu-pro (nep) sequence is part of the active site of egcase of rhodococcus sp. strain m-777. this paper describes the molecular cloning of a new egcase gene utilizing the nep sequence from the genomic library of rhodococcus sp. strain c9, which was clearly distinguishable from m-777 by 16s rdna analysis. c9 ... | 2000 | 10876169 |
| molecular analysis of surfactant-driven microbial population shifts in hydrocarbon-contaminated soil. | we analyzed the impact of surfactant addition on hydrocarbon mineralization kinetics and the associated population shifts of hydrocarbon-degrading microorganisms in soil. a mixture of radiolabeled hexadecane and phenanthrene was added to batch soil vessels. witconol sn70 (a nonionic, alcohol ethoxylate) was added in concentrations that bracketed the critical micelle concentration (cmc) in soil (cmc') (determined to be 13 mg g(-1)). addition of the surfactant at a concentration below the cmc' (2 ... | 2000 | 10877792 |
| substrate specificity of regiospecific desaturation of aliphatic compounds by a mutant rhodococcus strain. | substrate specificity of cis-desaturation of alipahtic compounds by resting cells of a mutant, rhodococcus sp. strain ksm-mt66, was examined. among substrates tested, the rhodococcal cells were able to convert n-alkanes (c13-c19), 1-chloroalkanes (c16 and c18), ethyl fatty acids (c14-c17) and alkyl (c1-c4) esters of palmitic acid to their corresponding unsaturated products of cis configuration. the products from n-alkanes and 1-chloroalkanes had a double bond mainly at the 9th carbon from their ... | 2000 | 10879483 |
| purification, characterization, and cdna cloning of a novel acidic endoglycoceramidase from the jellyfish, cyanea nozakii. | endoglycoceramidase (ec ) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids. we report here the purification, characterization, and cdna cloning of a novel endoglycoceramidase from the jellyfish, cyanea nozakii. the purified enzyme showed a single protein band estimated to be 51 kda on sds-polyacrylamide gel electrophoresis. the enzyme showed a ph optimum of 3.0 and was activated by triton x-100 and lubrol px but not by s ... | 2000 | 10882727 |
| a repeat-batch membrane bioreactor with a phase inversion for the desaturation of isopropyl palmitate by a mutant rhodococcus strain. | a repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a rhodococcus mutant. the addition of glutamate, thiamine, and mgso(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance of desaturation activity during cell recycling. an oil-in-water (o/w) type emulsion of the reaction medium was inverted to a water-in-oil (w/o) type by discharging the water phase f ... | 2000 | 10908790 |
| association of disease with isolation and virulence of rhodococcus equi from farm soil and foals with pneumonia. | to determine whether isolation and virulence of rhodococcus equi from soil and infected foals are associated with clinical disease. | 2000 | 10909463 |
| characterization of s-triazine herbicide metabolism by a nocardioides sp. isolated from agricultural soils. | atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. nine gram-positive bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from four farms in central canada. the strains were divided into two groups based on repetitive extragenic palindromic (rep)-pcr genomic fingerprinting with eric and boxa1r primers. based on 16s ribosomal dna sequence analysis, both groups were identified as nocardioides sp. strains. none ... | 2000 | 10919761 |
| rhodococcus l-phenylalanine dehydrogenase: kinetics, mechanism, and structural basis for catalytic specificity. | phenylalanine dehydrogenase catalyzes the reversible, pyridine nucleotide-dependent oxidative deamination of l-phenylalanine to form phenylpyruvate and ammonia. we have characterized the steady-state kinetic behavior of the enzyme from rhodococcus sp. m4 and determined the x-ray crystal structures of the recombinant enzyme in the complexes, e.nadh.l-phenylalanine and e.nad(+). l-3-phenyllactate, to 1.25 and 1.4 a resolution, respectively. initial velocity, product inhibition, and dead-end inhibi ... | 2000 | 10924111 |
| multiple respiratory bursts as a response to veratrate stress in rhodococcus erythropolis cells. | although rhodococcus spp. strains are able to degrade methoxyphenols by enzymatic means, the contact with veratric acid (3, 4-dimethoxybenzoic acid, hereafter called veratrate) is very stressful for the cells of rhodococcus erythropolis dsm 1069 (rh). within 5 min of contact veratrate in phosphate buffer, the emergence of many vacuoles was observed in the cell body and respiratory bursts, with violent endogenous oxygen uptake, took place several times during the 24 h incubation. during these pea ... | 2000 | 10924225 |
| pathogenicity of rhodococcus equi expressing a virulence-associated 20 kda protein (vapb) in foals. | rhodococcus equi strains of intermediate virulence (imv) for mice possess a 20kda protein designated virulence associated protein b (vapb) and a virulence plasmid of 79-100kb, and can be recovered from the submaxillary lymph nodes of pigs. the pathogenicity of such r. equi strains for foals is unknown. in this study, two foals, 42 and 43 days of age, were infected intratracheally with 10(6) and 10(9) cells of r. equi imv strain a5, respectively. the foal infected with 10(9) cells of strain a5 be ... | 2000 | 10925043 |
| propionicin sm1, a bacteriocin from propionibacterium jensenii df1: isolation and characterization of the protein and its gene. | we purified a bacteriocin from the cell-free supernatant of propionibacterium jensenii df1 isolated from swiss raw milk, and named it propionicin sm1. the heat-stable protein was strongly bactericidal against p. jensenii dsm20274. on the basis of the n-terminal amino acid sequence of the purified protein, a degenerate oligonucleotide probe was designed to locate and clone the corresponding gene of p. jensenii df1. it hybridized exclusively with the df1l-resident plasmid plme106, but not with chr ... | 2000 | 10930068 |
| lung nodular lesions in heart transplant recipients. | to describe the characteristics and etiology of lung nodules after heart transplantation (ht). during a 6-year period 147 patients received ht and 130 survived more than 1 week. nodular lesions were demonstrated after ht in 13 patients (10%). median age was 53 years, and all patients were male. nodules were detected 23 to 158 days after ht (median, 66 days). an etiologic diagnosis was made in all but 1 case: aspergillus (5), nocardia-rhodococcus (4), and cytomegalovirus (cmv) (3). previous sever ... | 2000 | 10930815 |
| characterisation of rhodococcus equi strains isolated from foals and from immunocompromised human patients. | the cultural, morphological, biochemical, serological characteristics and antibiotic susceptibility of 25 rhodococcus equi strains isolated from lungs and lung abscesses of pneumonic foals and 5 r. equi strains isolated from immuno-compromised human patients were examined. all r. equi strains showed common cultural, morphological and biochemical characteristics both with conventional tests and on the basis of their enzyme profile. the r. equi strains examined were resistant to penicillins with t ... | 2000 | 11402708 |
| detection of genes for alkane and naphthalene catabolism in rhodococcus sp. strain 1bn. | rhodococcus sp. 1bn was isolated from a contaminated site and showed various biodegradative capabilities. besides naphthalene, strain 1bn degraded medium- (c6) and long-chain alkanes (c16-c28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. the nucleotide sequence of an alk polymerase chain reaction (pcr) fragment revealed a 59% nucleotide homology to the pseudomonas oleovorans alkb gene. the nar fragments were highly homologous to genes coding for large and ... | 2000 | 11233165 |
| [cavitated pulmonary condensation by rhodococcus equi, in an hiv seropositive individual]. | the first clinical case described in portugal of a human infection by rhodococcus equi in a 35 year-old hiv1 seropositive i.v. drug user is presented. the patient was admitted to hospital due to clinical and radiological suspicion of tuberculosis. while in hospital, a rhodococcus equi cavitated lung infection was diagnosed. by applying a triple antibiotic therapy with erythromycin, rifampin and teicoplanin along with surgical excision of the lower lobe of the right lung, we were able to clinical ... | 2000 | 11234501 |
| chromosome topology and genome size of selected actinomycetes species. | information about the genome organization of actinomycetes species is restricted to a few genera: corynebacterium, mycobacterium, rhodococcus, saccharopolyspora and streptomyces. streptomyces species and saccharopolyspora erythraea were shown to contain a single linear 8 mb chromosome. in contrast, the corynebacterium, mycobacterium and rhodococcus species studied were demonstrated to possess a smaller (3 mb-6.5 mb) single circular chromosome. to investigate whether linear chromosome topology an ... | 2000 | 11386344 |
| genomic and phenomic differentiation of rhodococcus equi and related strains. | 16s rdna sequence and pyrolysis mass spectrometric analyses were carried out on representatives of rhodococcus equi and marker strains of genera that encompass mycolic acid containing actinomycetes. the r. equi strains formed a monophyletic clade within the evolutionary radiation occupied by members of the genera nocardia and rhodococcus. the 16s rdna sequence data also showed r. equi to be an heterogeneous taxon. this heterogeneity was underscored by the pyrolysis mass spectrometric data. these ... | 2000 | 11386356 |
| the diversity of extradiol dioxygenase (edo) genes in cresol degrading rhodococci from a creosote-contaminated site that express a wide range of degradative abilities. | analysis of the bacterial population of soil surface samples from a creosote-contaminated site showed that up to 50% of the culturable micro-organisms detected were able to utilise a mixture of cresols. from fifty different microbial isolates fourteen that could utilise more than one cresol isomer were selected and identified by 16s rrna analysis. eight isolates were rhodococcus strains and six were pseudomonas strains. in general, the rhodococcus strains exhibited a broader growth substrate ran ... | 2000 | 11386357 |
| characterization of is666, a newly described insertion element of mycobacterium avium. | the insertion sequence is666 was isolated from mycobacterium avium strain 101. is666 is a 1474 bp insertion sequence belonging to the is256 family, that includes is6120 from mycobacterium smegmatis, is1166 and is1295 from rhodococcus sp. igts8, ist2 from thiobacillus ferrooxidans, is256 from staphylococcus aureus, and isrm3 from rhizobium meliloti. is666 has 24 bp imperfect inverted repeats that fit the consensus described for the family, and generates 9 bp duplications upon insertion into the h ... | 2000 | 11471832 |
| biodegradation and conversion of alkanes and crude oil by a marine rhodococcus sp. | a hydrocarbon degrader isolated from a chronically oil-polluted marine site was identified as rhodococcus sp. on the basis of morphology, fatty acid methyl ester pattern, cell wall analysis, biochemical tests and g + c content of dna. it degraded up to 50% of the aliphatic fraction of assam crude oil, in seawater supplemented with 35 mm nitrogen as urea and 0.1 mm phosphorus as dipotassium hydrogen orthophosphate, after 72 h at 30 degrees c and 150 revolutions per minute. the relative percentage ... | 2000 | 11487058 |
| bioremediation of oil polluted aquatic systems and soils with novel preparation 'rhoder'. | this paper summarises the experience accumulated during the field application of biopreparation 'rhoder' (solely or in a combination with preliminary mechanical collection of free oil) for remediation of oil polluted aquatic systems and soils in the moscow region and western siberia during 1994-1999. it was demonstrated that 'rhoder' had a very high efficiency (>99%) for bioremediation of the open aquatic surfaces (100 m2 bay of the river chernaya, two 5,000 m2 lakes in vyngayakha) at initial le ... | 2000 | 11587442 |
| rapid characterisation of deep-sea actinomycetes for biotechnology screening programmes. | a continual need in natural product discovery is dereplication, that is the ability to exclude previously tested microorganisms from screening programmes. whole-cell fingerprinting techniques offer an ideal solution to this problem because of their rapidity and reproducibility, dependence on small samples, and automation. one such technique, curie-point pyrolysis mass spectrometry (pyms), has been deployed for the characterisation of a unique collection of actinomycetes recovered from pacific oc ... | 2000 | 10959565 |
| microbiology of the stalactites from grotta dei cervi, porto badisco, italy. | the active stalactites from grotta dei cervi, porto badisco, southeastern italy, were sampled to investigate the microbial communities present in these speleothems. sampling was carried out in a transect about 150 m long in the central gallery, where numerous gram-positive bacteria were isolated. actinomycetes of the genus streptomyces were the most abundant, followed by members of the genus bacillus. further isolates were assigned to the genera amycolatopsis, arthrobacter; agromyces. micrococcu ... | 2000 | 10963330 |
| accumulation and mobilization of storage lipids by rhodococcus opacus pd630 and rhodococcus ruber ncimb 40126. | the time course of the accumulation of triacylglycerols (tags) in rhodococcus opacus pd630 or of tags plus polyhydroxyalkanoates (pha) in rhodococcus ruber ncimb 40126 with gluconate or glucose as carbon source, respectively, was studied. in addition, we examined the mobilization of these storage compounds in the absence of a carbon source. r. opacus accumulated tags only after the exhaustion of ammonium in the medium, and, with a fixed concentration of the carbon source, the amounts of tags in ... | 2000 | 10968636 |
| distribution of alkb genes within n-alkane-degrading bacteria. | fifty-four bacterial strains belonging to 37 species were tested for their ability to assimilate short chain and/or medium chain liquid n-alkanes. a gene probe derived from the alkb gene of pseudomonas oleovorans atcc 29347 was utilized in hybridization experiments. results of southern hybridization of pcr-amplificates were compared with those of colony hybridization and dot blot hybridization. strongest signals were received only from gram-negative bacteria growing solely with short n-alkanes ( ... | 2000 | 10971768 |
| effects of prior feeding on pharmacokinetics and estimated bioavailability after oral administration of a single dose of microencapsulated erythromycin base in healthy foals. | to determine effects of prior feeding on pharmacokinetics and estimated bioavailability of orally administered microencapsulated erythromycin base (meb) in healthy foals. | 2000 | 10976728 |
| cloning and expression of the nitrile hydratase and amidase genes from bacillus sp. br449 into escherichia coli. | a moderate thermophile, bacillus sp. br449 was previously shown to exhibit a high level of nitrile hydratase (nhase) activity when growing on high levels of acrylonitrile at 55 degrees c. in this report, we describe the cloning of a 6.1 kb sali dna fragment encoding the nhase gene cluster of br449 into escherichia coli. nucleotide sequencing revealed six orfs encoding (in order), two unidentified putative proteins, amidase, nhase beta- and alpha-subunits and a small putative protein of 101 amino ... | 2000 | 10978771 |
| in vitro effects of sterculic acid on lipid biosynthesis in rhodococcus opacus strain pd630 and isolation of mutants defective in fatty acid desaturation. | the in vivo effects of sterculic acid methyl ester on triacylglycerol fatty acid composition in the oleaginous, hydrocarbon-degrading bacterium r. opacus strain pd630 was investigated. sterculic acid, a cyclopropene fatty acid and an inhibitor of the stearoyl-coa desaturase system, strongly inhibited the synthesis of monoenic fatty acids, of saturated fatty acids with more than 16 carbon atoms and of odd-numbered fatty acids when added to the culture medium. in addition, chemical mutagenesis and ... | 2000 | 10981688 |
| leafy gall formation is controlled by fasr, an arac-type regulatory gene in rhodococcus fascians. | rhodococcus fascians can interact with many plant species and induce the formation of either leafy galls or fasciations. to provoke symptoms, r. fascians strain d188 requires pathogenicity genes that are located on a linear plasmid, pfid188. the fas genes are essential for virulence and constitute an operon that encodes, among other functions, a cytokinin synthase gene. expression of the fas genes is induced by extracts of infected plant tissue only. we have isolated an arac-type regulatory gene ... | 2000 | 11004184 |
| the iron dependent regulatory protein ider (dtxr) of rhodococcus equi. | this paper reports the presence of an ider gene, which encodes an iron-dependent regulatory protein, in rhodococcus erythropolis and in the intracellular pathogen rhodococcus equi. the ider gene of the latter encoded a protein of 230 amino acids with a molecular mass of 25619. the alpha-helices forming the helix-turn-helix motif of the r. equi protein were identical to those of the dtxr protein of corynebacterium diphtheriae, which is an ider homologue. this indicates that the two proteins bind ... | 2000 | 11004391 |
| [growth of rhodococcus opacus on mixtures of monohalogenated benzenes and phenols]. | the growth of rhodococcus opacus gm-14 on mixtures of 2-chloro- and 2-bromophenol, of 4-chloro, 4-bromo-, and 4-iodophenol, and of chloro-, bromo-, and iodobenzenes was accompanied by consumption of the substrates and excretion of halogen ions to the medium. during the growth on monochlorophenols, the substrates were consumed sequentially in the following order: 4-chloro-, 3-chloro-, and then 2-chlorophenol. chlorine ions were excreted in a two-phase manner in amounts comprising 79% of the theor ... | 2000 | 11008684 |
| bacterial phosphating of mild (unalloyed) steel. | mild (unalloyed) steel electrodes were incubated in phosphate-buffered cultures of aerobic, biofilm-forming rhodococcus sp. strain c125 and pseudomonas putida mt2. a resulting surface reaction leading to the formation of a corrosion-inhibiting vivianite layer was accompanied by a characteristic electrochemical potential (e) curve. first, e increased slightly due to the interaction of phosphate with the iron oxides covering the steel surface. subsequently, e decreased rapidly and after 1 day reac ... | 2000 | 11010888 |
| microbial degradation of the multiply branched alkane 2,6,10,15,19, 23-hexamethyltetracosane (squalane) by mycobacterium fortuitum and mycobacterium ratisbonense. | among several bacterial species belonging to the general gordonia, mycobacterium, micromonospora, pseudomonas, and rhodococcus, only two mycobacterial isolates, mycobacterium fortuitum strain nf4 and the new isolate mycobacterium ratisbonense strain sd4, which was isolated from a sewage treatment plant, were capable of utilizing the multiply branched hydrocarbon squalane (2,6,10,15,19, 23-hexamethyltetracosane) and its analogous unsaturated hydrocarbon squalene as the sole carbon source for grow ... | 2000 | 11010899 |
| stable isotope incorporation triples the upper mass limit for determination of elemental composition by accurate mass measurement. | by comparing electrospray ionization fourier-transform ion cyclotron resonance (ft-icr) mass spectra and collision-induced dissociation (cid) ft-icr mass spectra of a phospholipid (851 da) extracted from natural abundance and 99% 13c bacterial growth media, we are able to reduce its number of possible elemental compositions (based on +/-10 ppm externally calibrated mass accuracy and biologically relevant compositional constraints) from 394 to 1. the basic idea is simply that the mass of a molecu ... | 2000 | 11014445 |
| immunohistochemical detection of virulence-associated rhodococcus equi antigens in pulmonary and intestinal lesions in horses. | two horses with rhodococcus equi infection were examined post mortem by an immunohistochemical method (peroxidase-antiperoxidase; pap) with a monoclonal antibody (mab 10g5) to the 15-17 kda antigen of r. equi. one of the horses was also examined bacteriologically, r. equi being isolated in culture. immunolabelling with this mab was marked and widespread. on the other hand, the immunohistochemical reactivity of infected macrophages with a polyclonal antibody specific for lysozyme was slight. thus ... | 2000 | 11032673 |
| characterization of the 20s proteasome from the actinomycete frankia. | frankia is an actinomycete that fixes atmospheric nitrogen in symbiotic association with the root systems of a variety of non-leguminous plants, denominated actinorhizal plants. information on the biology of proteolysis in frankia is almost non-existent as it is extremely difficult to grow this organism. we have purified 20s proteasomes from frankia strain acn14a/ts-r. it is composed of one alpha-subunit and one beta-subunit, which assemble into the canonical structure of four rings of seven sub ... | 2000 | 10652097 |
| the rhodococcus fascians-plant interaction: morphological traits and biotechnological applications. | rhodococcus fascians is a gram-positive bacterium that infects dicotyledonous and monocotyledonous plants, leading to an alteration in the normal growth process of the host. the disease results from the modulation of the plant hormone balances, and cytokinins are thought to play an important role in the induction of symptoms. generally, on the aerial parts of the plants, existing meristems were found to be most sensitive to the action of r. fascians, but, depending on the infection procedure, di ... | 2000 | 10664130 |
| light inhibits rifampicin inactivation and reduces rifampicin resistance due to a cloned mycobacterial adp-ribosylation gene. | rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. most strains of mycobacterium are able to inactivate this antibiotic by ribosylation via an adp-ribosylated intermediate. we found that this inactivation was inhibited by light at levels similar to those prevailing in laboratory environments. rifampicin resistance arising from the cloned adp-ribosyl transferase was also greatly diminished at these light levels. the cloned rhodococcus equi monooxygenas ... | 2000 | 10612740 |
| rhodococcus equi nosocomial meningitis cured by levofloxacin and shunt removal. | 2000 | 10619769 | |
| cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene (narb) from rhodococcus sp. ncimb12038. | rhodococcus sp. ncimb112038 can utilize naphthalene as its sole carbon and energy source. the gene encoding cis-naphthalene dihydrodiol dehydrogenase (narb) of this strain has been cloned and sequenced. expression of ncimb12038 cis-naphthalene dihydrodiol dehydrogenase was demonstrated in escherichia coli cells. narb encodes a putative protein of 271 amino acids and shares 39% amino acid identity with the cis-naphthalene dihydrodiol dehydrogenase from pseudomonas putida g7. comparison of narb wi ... | 2000 | 10620687 |
| cobalt-substituted fe-type nitrile hydratase of rhodococcus sp. n-771. | when the genes encoding alpha and beta subunits of fe-type nitrile hydratase (nhase) from rhodococcus sp. n-771 were expressed in escherichia coli in co-supplemented medium without co-expression of the nhase activator, the nhase specifically incorporated not fe but co ion into the catalytic center. the produced co-substituted enzyme exhibited rather weak nhase activity, initially. however, the activity gradually increased by the incubation with an oxidizing agent, potassium hexacyanoferrate. the ... | 2000 | 10631329 |
| biochemical identification and biophysical characterization of a channel-forming protein from rhodococcus erythropolis. | organic solvent extracts of whole cells of the gram-positive bacterium rhodococcus erythropolis contain a channel-forming protein. it was identified by lipid bilayer experiments and purified to homogeneity by preparative sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page). the pure protein had a rather low molecular mass of about 8.4 kda, as judged by sds-page. sds-resistant oligomers with a molecular mass of 67 kda were also observed, suggesting that the channel is formed by ... | 2000 | 10633112 |
| humus bacteria of norway spruce stands: plant growth promoting properties and birch, red fescue and alder colonizing capacity. | we studied the potential of the humus layer of the norway spruce stands to supply beneficial rhizobacteria to birch (betula pendula), alder (alnus incana) and fescue grass (festuca rubra), representatives of pioneer vegetation after clear-cutting of the coniferous forest. axenically grown seedlings of these species were inoculated with the acid spruce humus, ph 3.7-5.3. actinorhizal propagules, capable of nodulating alder, were present in high density (10(3) g(-1)) in humus of long-term limed pl ... | 2000 | 10640667 |
| operon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of paenibacillus sp. a11-2. | paenibacillus a11-2 can efficiently cleave two carbon&bond;sulfur bonds in dibenzothiophene (dbt) and alkyl dbts, which are refractory by conventional petroleum hydrodesulfurization, to remove sulfur atom at high temperatures. an 8.7-kb dna fragment containing the genes for the dbt desulfurizing enzymes of a11-2 was cloned in escherichia coli and characterized. heterologous expression analysis of the deletion mutants identified three open reading frames that were required for the desulfurization ... | 2000 | 10733908 |
| isolation of rare opportunistic pathogens in hungary: case report and short review of the literature. rhodococcus equi. | rhodococcus equi is a well-established pathogen in foal pneumonia and is increasingly recognized as a pathogen in immunocompromised humans. we have isolated a gram-positive coccobacillus from 8 blood samples and lung tissues of a renal transplant patient. colony morphology, growth in lowenstein-jensen medium, 21 biochemical reactions, the characteristic morphological cycle (coccus-rod-coccus) and the camp test established the r. equi diagnosis. histological studies of 2 lung biopsy specimens rev ... | 2000 | 10735185 |
| roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways. | the haloalkane-degrading bacteria rhodococcus rhodochrous ncimb13064, pseudomonas pavonaceae 170, and mycobacterium sp. strain gp1 share a highly conserved haloalkane dehalogenase gene (dhaa). here, we describe the extent of the conserved dhaa segments in these three phylogenetically distinct bacteria and an analysis of their flanking sequences. the dhaa gene of the 1-chlorobutane-degrading strain ncimb13064 was found to reside within a 1-chlorobutane catabolic gene cluster, which also encodes a ... | 2000 | 10735862 |
| production of isopropyl cis-6-hexadecenoate by regiospecific desaturation of isopropyl palmitate by a double mutant of a rhodococcus strain. | resting cells of a double mutant noted as ksm-mt66, derived from rhodococcus sp. strain ksm-b-3 by uv irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. addition of sodium glutamate (1.0%), mg so4 (2 mm), and thiamine (2 mm) increased the productivity of the unsaturated product in phosphate buffer. optimal temperature and ph for the reaction were around 26 degrees c and 7, respectively. under the optimized conditions, more than 50 g/l of is ... | 2000 | 10737199 |
| mycolic acids from rhodococcus, gordonia, and dietzia. | the mycolic acids from 11 species of rhodococcus, seven species of gordonia, and one species of dietzia were analyzed using capillary gas chromatography and mass spectrometry (glc/ms). all strains tested in this study were divided into three groups according to the degree of double bonds and the average carbon number (av.nc.) of their mycolic acids. the genus gordonia belongs to the first group possessing an av.nc. in the upper 50s and 60s with 0 to 5 double bonds. some rhodococcus species posse ... | 2000 | 10739337 |
| characterization of the protocatechuic acid catabolic gene cluster from streptomyces sp. strain 2065. | protocatechuate 3,4-dioxygenase (ec 1.13.11.3) catalyzes the ring cleavage step in the catabolism of aromatic compounds through the protocatechuate branch of the beta-ketoadipate pathway. a protocatechuate 3,4-dioxygenase was purified from streptomyces sp. strain 2065 grown in p-hydroxybenzoate, and the n-terminal sequences of the beta- and alpha-subunits were obtained. pcr amplification was used for the cloning of the corresponding genes, and dna sequencing of the flanking regions showed that t ... | 2000 | 10742233 |
| nitrile hydratase and amidase from rhodococcus rhodochrous hydrolyze acrylic fibers and granular polyacrylonitriles. | rhodococcus rhodochrous ncimb 11216 produced nitrile hydratase (320 nkat mg of protein(-1)) and amidase activity (38.4 nkat mg of protein(-1)) when grown on a medium containing propionitrile. these enzymes were able to hydrolyze nitrile groups of both granular polyacrylonitriles (pan) and acrylic fibers. nitrile groups of pan40 (molecular mass, 40 kda) and pan190 (molecular mass, 190 kda) were converted into the corresponding carbonic acids to 1.8 and 1.0%, respectively. in contrast, surfacial n ... | 2000 | 10742253 |
| successful treatment of rhodococcus equi pulmonary infection in a renal transplant recipient. | the rhodococcus is a mycobacterium-like organism which is normally a pathogen in foals. it usually spreads by direct contact or by aerosol from horse faeces and causes pyogranulomatous pulmonary infections. occasionally, it acts opportunistically to infect immuno-compromised human hosts, most commonly those with the acquired immune deficiency syndrome (aids). here we report a pulmonary infection by rhodococcus equi in a renal transplant recipient who was successfully treated. the literature on t ... | 2000 | 10743428 |
| [adaptation of acrylamide producer rhodococcus rhodochrous m8 to change in ammonium concentration in medium]. | the mechanism of adaptation of the acrylamide producing strain rhodococcus rhodochrous m8 to changes in ammonium concentrations in the medium was studied. an increase in the content of ammonium in the medium changed the activity of glutamine synthetase (gs) (ec 6.3.1.2) and glutamine dehydrogenase (gd) (ec 1.4.1.4), the enzymes of ammonium assimilation, as well as the activities of enzymes responsible for nitrile utilization: nitrile hydratase (ec 4.2.1.84) and amidase (ec 3.5.1.4). this also ca ... | 2000 | 10752079 |
| [storage of industrial microorganisms entrapped into polymer matrices]. | our study of the techniques of long-term storage of the biomass of various strains of microorganisms, which cause breakdown or transformation of synthetic organic compounds, demonstrates that desiccated agar beads with immobilized microbial cells can be used for this purpose. in addition, the cells can be stored in desiccated matrices of agar or polyvinyl alcohol, coating synthetic cords. such dry biocatalysts may be used for quick starting of bioreactors and in other biotechnological processes. ... | 2000 | 10752086 |
| characterization of the naphthalene-degrading bacterium, rhodococcus opacus m213. | bacterial strain m213 was isolated from a fuel oil-contaminated soil in idaho, usa, by growth on naphthalene as a sole source of carbon, and was identified as rhodococcus opacus m213 by 16s rdna sequence analysis and growth on substrates characteristic of this species. m213 was screened for growth on a variety of aromatic hydrocarbons, and growth was observed only on simple 1 and 2 ring compounds. no growth or poor growth was observed with chlorinated aromatic compounds such as 2,4-dichloropheno ... | 2000 | 10754253 |
| heterologous expression of bacterial epoxyalkane:coenzyme m transferase and inducible coenzyme m biosynthesis in xanthobacter strain py2 and rhodococcus rhodochrous b276. | coenzyme m (com) (2-mercaptoethanesulfonic acid) biosynthesis is shown to be coordinately regulated with the expression of the enzymes of alkene and epoxide metabolism in the propylene-oxidizing bacteria xanthobacter strain py2 and rhodococcus rhodochrous strain b276. these results provide the first evidence for the involvement of com in propylene metabolism by r. rhodochrous and demonstrate for the first time the inducible nature of eubacterial com biosynthesis. | 2000 | 10762269 |
| synthesis and antifungal activity of rhodopeptin analogues. 1. modification of the east and south amino acid moieties. | structure-activity relationships of the east and south amino acid modified analogues of rhodopeptins, novel antifungal cyclic tetrapeptides isolated from rhodococcus species mer-n1033, have been investigated. it was observed that a basic amino acid moiety (lysine or ornithine) as the east amino acid and a hydrophobic and bulky neutral amino acid (i.e., gamma-methylleucine) as the south amino acid were indispensable structure motifs for antifungal activity of rhodopeptin analogues. | 2000 | 10768200 |
| synthesis and antifungal activity of rhodopeptin analogues. 2. modification of the west amino acid moiety. | structure-activity relationships of the west amino acid modified analogues of rhodopeptins, novel antifungal tetrapeptide isolated from rhodococcus species mer-n1033, have been investigated. among the analogues synthesized, 2,2-difluoro and 2-hydroxy derivatives retained the antifungal activity with better physical properties, i.e., solubility or acute toxicity. | 2000 | 10768201 |
| purification and characterization of a baeyer-villiger mono-oxygenase from rhodococcus erythropolis dcl14 involved in three different monocyclic monoterpene degradation pathways. | a baeyer-villiger mono-oxygenase (bvmo), catalysing the nadph- and oxygen-dependent oxidation of the monocyclic monoterpene ketones 1-hydroxy-2-oxolimonene, dihydrocarvone and menthone, was purified to homogeneity from rhodococcus erythropolis dcl14. monocyclic monoterpene ketone mono-oxygenase (mmkmo) is a monomeric enzyme of molecular mass 60 kda. it contains 1 mol of fad/monomer as the prosthetic group. the n-terminal amino acid sequence showed homology with many other nadph-dependent and fad ... | 2000 | 10769172 |
| pulmonary infection in human immunodeficiency virus-infected patients in taiwan. | pulmonary infection is a major cause of morbidity and mortality in human immunodeficiency virus (hiv)-infected patients. | 2000 | 10770026 |
| mics of oxazolidinones for rhodococcus equi strains isolated from humans and animals. | eperezolid and linezolid are representatives of a new class of orally active, synthetic antimicrobial agents. the in vitro activity values (mics) of linezolid, eperezolid, and comparator antibiotics against 102 strains of rhodococcus equi isolated from humans and animals were determined. linezolid was more active than eperezolid against the strains tested; premafloxacin was the most active comparator antibiotic. | 2000 | 10770781 |
| [the degree of halophily in rhodococcus erythropolis and halobacterium salinarum depends on the partial pressure of oxygen]. | 2000 | 10776634 | |
| effects of non-ionic surfactants on the uptake and hydrolysis of fluoresceindiacetate by alkane-oxidizing bacteria. | biological effects of non-ionic surfactants on alkane-oxidizing bacteria were studied by assessing their influence on the uptake of prefluorochrome fluoresceindiacetate (fda) and its intracellular hydrolysis to fluorescein. both decreasing and increasing rates of hydrolysis as a consequence of the presence of surfactants were observed. the surfactants influenced the uptake of fda, but not its intracellular hydrolysis. the effects of the surfactants on the uptake rate depended strongly on the str ... | 2000 | 10779877 |
| haloalkane-utilizing rhodococcus strains isolated from geographically distinct locations possess a highly conserved gene cluster encoding haloalkane catabolism. | the sequences of the 16s rrna and haloalkane dehalogenase (dhaa) genes of five gram-positive haloalkane-utilizing bacteria isolated from contaminated sites in europe, japan, and the united states and of the archetypal haloalkane-degrading bacterium rhodococcus sp. strain ncimb13064 were compared. the 16s rrna gene sequences showed less than 1% sequence divergence, and all haloalkane degraders clearly belonged to the genus rhodococcus. all strains shared a completely conserved dhaa gene, suggesti ... | 2000 | 10781539 |
| nicotinoprotein (nadh-containing) alcohol dehydrogenase from rhodococcus erythropolis dsm 1069: an efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes. | extracts from benzyl-alcohol-grown rhodococcus erythropolis dsm 1069 showed nad(p)-independent, n,n-dimethyl-4-nitrosoaniline (ndma)-dependent alcohol dehydrogenase activity. the enzyme exhibiting this activity was purified to homogeneity and characterized. it appears to be a typical nicotinoprotein as it contains tightly bound nadh acting as cofactor instead of coenzyme. other characteristics indicate that it is highly similar to the known nicotinoprotein alcohol dehydrogenase (np-adh) from amy ... | 2000 | 10784035 |
| targeted disruption of the kstd gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of rhodococcus erythropolis strain sq1. | microbial phytosterol degradation is accompanied by the formation of steroid pathway intermediates, which are potential precursors in the synthesis of bioactive steroids. degradation of these steroid intermediates is initiated by delta(1)-dehydrogenation of the steroid ring structure. characterization of a 2.9-kb dna fragment of rhodococcus erythropolis sq1 revealed an open reading frame (kstd) showing similarity with known 3-ketosteroid delta(1)-dehydrogenase genes. heterologous expression of k ... | 2000 | 10788377 |
| identification of fluoropyrogallols as new intermediates in biotransformation of monofluorophenols in rhodococcus opacus 1cp. | the transformation of monofluorophenols by whole cells of rhodococcus opacus 1cp was investigated, with special emphasis on the nature of hydroxylated intermediates formed. thin-layer chromatography, mass spectrum analysis, and (19)f nuclear magnetic resonance demonstrated the formation of fluorocatechol and trihydroxyfluorobenzene derivatives from each of three monofluorophenols. the (19)f chemical shifts and proton-coupled splitting patterns of the fluorine resonances of the trihydroxyfluorobe ... | 2000 | 10788394 |