Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| elastase digests: new ammunition for shotgun membrane proteomics. | despite many advances in membrane proteomics during the last decade the fundamental problem of accessing the transmembrane regions itself has only been addressed to some extent. the present study establishes a method for the nano-lc-based analysis of complex membrane proteomes on the basis of a methanolic porcine pancreatic elastase digest to increase transmembrane coverage. halobacterium salinarium purple and corynebacterium glutamicum membranes were successfully analyzed by using the new proto ... | 2009 | 19116210 |
| characterization of the laci-type transcriptional repressor rbsr controlling ribose transport in corynebacterium glutamicum atcc 13032. | the gene products of the rbsracbd (rbs) operon of c. glutamicum (cg1410-cg1414) encode a ribose-specific atp-binding cassette (abc) transport system and its corresponding regulatory protein (rbsr). deletion of the structural genes rbsacbd prohibited ribose uptake. deletion of the regulatory gene rbsr resulted in an increased mrna level of the whole operon. analysis of the promoter region of the rbs operon by electrophoretic mobility shift assays identified a catabolite-responsive element (cre)-l ... | 2009 | 19118356 |
| metabolic engineering of the l-valine biosynthesis pathway in corynebacterium glutamicum using promoter activity modulation. | the previously constructed strain corynebacterium glutamicumilvnm13 with acetohydroxy acid synthase, resistant to inhibition by all three branched-chain amino acids (l-valine, l-isoleucine and l-leucine), was used as a basis to develop a new type of valine producer by genetic engineering. the main strategy was to modulate expression of the genes involved in the biosynthesis of branched-chain amino acids. the activity of the promoters p-ilvd (dihydroxyacid dehydratase) and p-ilve (transaminase) w ... | 2009 | 19121344 |
| the serine hydroxymethyltransferase gene glya in corynebacterium glutamicum is controlled by glyr. | serine hydroxymethyltransferase (shmt) occupies a central position in one-carbon metabolism, and we here study its regulation in corynebacterium glutamicum. enzyme quantifications revealed an about 3-fold increase of shmt activity during exponential growth with a further increase at the onset of the stationary phase. the shmt encoding glya gene was shown to be transcribed as a monocistronic mrna, and its transcriptional start site was determined. using dna affinity chromatography the regulator g ... | 2009 | 19124047 |
| reengineering of a corynebacterium glutamicum l-arginine and l-citrulline producer. | toward the creation of a robust and efficient producer of l-arginine and l-citrulline (arginine/citrulline), we have performed reengineering of a corynebacterium glutamicum strain by using genetic information of three classical producers. sequence analysis of their arg operons identified three point mutations (argr123, argg92(up), and argg45) in one producer and one point mutation (argb26 or argb31) in each of the other two producers. reconstitution of the former three mutations or of each argb ... | 2009 | 19139237 |
| reliable transfer of transcriptional gene regulatory networks between taxonomically related organisms. | transcriptional regulation of gene activity is essential for any living organism. transcription factors therefore recognize specific binding sites within the dna to regulate the expression of particular target genes. the genome-scale reconstruction of the emerging regulatory networks is important for biotechnology and human medicine but cost-intensive, time-consuming, and impossible to perform for any species separately. by using bioinformatics methods one can partially transfer networks from we ... | 2009 | 19146695 |
| recombinant glutamine synthetase (gs) from c. glutamicum existed as both hexamers & dedocamers and c-terminal his-tag enhanced inclusion bodies formation in e. coli. | in order to investigate the effect of his-tag on glutamine synthetase (gs, ec 6.3.1.2) from corynebacterium glutamicum, recombinant escherichia coli strains overexpressing gsim, hgsim (gs fused with n-terminal his-tag), gsimh (gs fused with c-terminal his-tag), and hgsimh (gs fused with n-terminal & c-terminal his-tags) were constructed, respectively. under similar expression conditions, gsim and hgsim were partially solubly expressed; no soluble gsimh and hgsimh were observed, based on the resu ... | 2009 | 19148777 |
| maturing dynamics of surface microflora in fontina pdo cheese studied by culture-dependent and -independent methods. | to study the evolution of rind microbial communities in fontina pdo cheese. | 2009 | 19054234 |
| effect of ph on the binding mechanisms in biosorption of reactive orange 16 by corynebacterium glutamicum. | binding mechanisms of reactive orange 16 (ro 16) by the protonated waste biomass of corynebacterium glutamicum were investigated. the solution ph was found to strongly influence the uptake of ro 16 by c. glutamicum. the biosorption of the dye was reversible at ph <7 but irreversible under basic ph conditions. at acidic ph, the electrostatic interaction was found to be a major binding mechanism. the maximum sorption capacities of the biomass were evaluated to be 156.6+/-6.2 and 64.0+/-2.4 mg/g at ... | 2009 | 19062035 |
| a 2d reversed-phase x ion-pair reversed-phase hplc-maldi tof/tof-ms approach for shotgun proteome analysis. | the separation of complex peptide mixtures in shotgun proteome analysis using a 2d separation scheme encompassing reversed-phase x ion-pair reversed-phase (ip-rp) liquid chromatography coupled online to electrospray ion trap mass spectrometry (ms) has been shown earlier to be superior in terms of separation efficiency and technical robustness compared to the classically used separation scheme encompassing strong cation exchange x ip-rp-chromatography in shotgun proteome analysis. in the present ... | 2009 | 19066860 |
| identification of new secreted proteins and secretion of heterologous amylase by c. glutamicum. | in this study, secreted corynebacterium glutamicum proteins were investigated by two-dimensional gel electrophoresis. around 100 spots observed in the ph range 4.5-5.5 had molecular masses that varied from 10 to 50 kda. upon n-terminal amino acid sequence analysis by edman degradation, two of them were hits to two hypothetical proteins encoded by cgr_1176 and cgr_2070 on c. glutamicum r genome, respectively. active-form alpha-amylase derived from geobacillus stearothermophilus was successfully s ... | 2009 | 19066885 |
| tatabc overexpression improves corynebacterium glutamicum tat-dependent protein secretion. | the twin-arginine translocation (tat) pathway in corynebacterium glutamicum has been described previously. the minimal functional tat system in c. glutamicum required tata and tatc but did not require tatb, although this component was required for maximal efficiency of tat-dependent secretion. we previously demonstrated that chryseobacterium proteolyticum pro-protein glutaminase (pro-pg) and streptomyces mobaraensis pro-transglutaminase (pro-tg) could be secreted via the tat pathway in c. glutam ... | 2009 | 19074606 |
| l-valine production during growth of pyruvate dehydrogenase complex-deficient corynebacterium glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator sugr. | pyruvate dehydrogenase complex-deficient strains of corynebacterium glutamicum produce l-valine from glucose only after depletion of the acetate required for growth. here we show that inactivation of the deor-type transcriptional regulator sugr or replacement of acetate by ethanol already in course of the growth phase results in efficient l-valine production. | 2009 | 19088318 |
| the whca gene plays a negative role in oxidative stress response of corynebacterium glutamicum. | in this study, we analyzed the whca gene from corynebacterium glutamicum, which codes for a homologue of the whib-family of proteins. deletion of the gene did not affect the growth of the mutant cells, indicating that the whca gene was not essential under ordinary growth conditions. however, cells overexpressing the protein not only showed retarded growth as compared with the wild-type or the deltawhca mutant cells but also showed increased sensitivity to a variety of oxidants, such as diamide, ... | 2009 | 19016879 |
| direct production of cadaverine from soluble starch using corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase. | here, we demonstrated the one-step production of cadaverine from starch using a corynebacterium glutamicum strain coexpressing streptococcus bovis 148 alpha-amylase (amya) and escherichia coli k-12 lysine decarboxylase (cada). we constructed the e. coli-c. glutamicum shuttle vector, which produces cada under the control of the high constitutive expression (hce) promoter, and transformed this vector into c. glutamicum css secreting amya. the engineered c. glutamicum expressed both cada and amya, ... | 2009 | 18989633 |
| crystal structure of the caseinolytic protease gene regulator, a transcriptional activator in actinomycetes. | human pathogens of the genera corynebacterium and mycobacterium possess the transcriptional activator clgr (clp gene regulator) which in corynebacterium glutamicum has been shown to regulate the expression of the clpcp protease genes. clgr specifically binds to pseudo-palindromic operator regions upstream of clpc and clpp1p2. here, we present the first crystal structure of a clgr protein from c. glutamicum. the structure was determined from two different crystal forms to resolutions of 1.75 and ... | 2009 | 19019826 |
| identification and characterization of a bacterial transport system for the uptake of pyruvate, propionate, and acetate in corynebacterium glutamicum. | the metabolism of monocarboxylic acids is of central importance for bacteria in their natural habitat as well as during biotechnological production. although biosynthesis and degradation are well understood, the transport of such compounds is still a matter of discussion. here we present the identification and characterization of a new transport system in corynebacterium glutamicum with high affinity for acetate and propionate and with lower affinity for pyruvate. biochemical analysis of this mo ... | 2009 | 19028892 |
| characterization of crustins from the hemocytes of the spider crab, hyas araneus, and the red king crab, paralithodes camtschaticus. | crustins are distributed across the decapods and are believed to play a significant part in the humoral defense system of their host. in this study, two crustin isoforms from hyas araneus hemocytes were purified and tested for antimicrobial activity against selected microorganisms. they show both antibacterial and antifungal activity, with highest activity against the gram-positive bacteria corynebacterium glutamicum. sequencing of the transcripts showed them to have a mature peptide of 90 amino ... | 2009 | 19041340 |
| a combination of metabolome and transcriptome analyses reveals new targets of the corynebacterium glutamicum nitrogen regulator amtr. | the effects of a deletion of the amtr gene, encoding the master regulator of nitrogen control in corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric acid cycle, and most amino acid pools. not all of these alterations could be attributed to changes at the level of mrna and must be caused by posttranscriptional regulatory processes. ... | 2009 | 19041910 |
| dual transcriptional control of the acetaldehyde dehydrogenase gene ald of corynebacterium glutamicum by rama and ramb. | corynebacterium glutamicum has been shown to grow with ethanol as the sole or as additional carbon and energy source and accordingly, to possess both alcohol dehydrogenase and acetaldehyde dehydrogenase (aldh) activities, which are responsible for the two-step ethanol oxidation to acetate. here we identify and functionally analyze the c. glutamicum aldh gene (cg3096, ald), its expression and its regulation. directed inactivation of the chromosomal ald gene led to the absence of detectable aldh a ... | 2009 | 19041911 |
| involvement of the luxr-type transcriptional regulator rama in regulation of expression of the gapa gene, encoding glyceraldehyde-3-phosphate dehydrogenase of corynebacterium glutamicum. | sugr, rama, glxr, gntr1, and a marr-type transcriptional regulator bind to the promoter region of the gapa gene encoding glyceraldehyde-3-phosphate dehydrogenase (gapdh), essential for glycolysis in corynebacterium glutamicum. we previously showed that sugr, a transcriptional repressor of phosphotransferase system genes for the sugar transport system, is involved in the downregulation of gapa expression in the absence of sugar. in this study, the role of rama in the expression of the gapa gene w ... | 2009 | 19047347 |
| acetohydroxyacid synthase, a novel target for improvement of l-lysine production by corynebacterium glutamicum. | the influence of acetohydroxy acid synthase (ahas) on l-lysine production by corynebacterium glutamicum was investigated. an ahas with a deleted c-terminal domain in the regulatory subunit ilvn was engineered by truncating the ilvn gene. compared to the wild-type ahas, the newly constructed enzyme showed altered kinetic properties, i.e., (i) an about twofold-lower k(m) for the substrate pyruvate and an about fourfold-lower v(max); (ii) a slightly increased k(m) for the substrate alpha-ketobutyra ... | 2009 | 19047397 |
| in silico genome-scale reconstruction and validation of the corynebacterium glutamicum metabolic network. | a genome-scale metabolic model of the gram-positive bacteria corynebacterium glutamicum atcc 13032 was constructed comprising 446 reactions and 411 metabolites, based on the annotated genome and available biochemical information. the network was analyzed using constraint based methods. the model was extensively validated against published flux data, and flux distribution values were found to correlate well between simulations and experiments. the split pathway of the lysine synthesis pathway of ... | 2009 | 18985611 |
| activity of exporters of escherichia coli in corynebacterium glutamicum, and their use to increase l-threonine production. | l-threonine is an important biotechnological product and corynebacterium glutamicum is able to synthesize and accumulate this amino acid to high intracellular levels. we here use four exporters of escherichia coli and show that three of them operate in c. glutamicum, with rhta and rhtc being the most effective. whereas rhta was unspecific, resulting in l-homoserine together with l-threonine excretion, this was not the case with rhtc. expression of rhtc reduced the intracellular l-threonine conce ... | 2009 | 18594129 |
| ramb is an activator of the pyruvate dehydrogenase complex subunit e1p gene in corynebacterium glutamicum. | in corynebacterium glutamicum, the transcriptional regulator ramb negatively controls the expression of genes involved in acetate metabolism. here we show that during growth in media containing glucose and in complex medium without glucose ramb activates expression of the acee gene, encoding the e1p subunit of the pyruvate dehydrogenase complex. thus, ramb functions both as repressor and as activator in c. glutamicum. | 2009 | 17890844 |
| pcao positively regulates pcahg of the beta-ketoadipate pathway in corynebacterium glutamicum. | we identified a new regulator, pcao, which is involved in regulation of the protocatechuate (pca) branch of the beta-ketoadipate pathway in corynebacterium glutamicum. pcao is an atypical large atp-binding luxr family (lal)-type regulator and does not have a walker a motif. a mutant of c. glutamicum in which pcao was disrupted (res167deltapcao) was unable to grow on pca, and growth on pca was restored by complementation with pcao. both an enzymatic assay of pca 3,4-dioxygenase activity (encoded ... | 2010 | 20081038 |
| the regulator rama influences cmyta transcription and cell morphology of corynebacterium ammoniagenes. | rama plays a regulatory role for acetate utilization and s-layer biosynthesis in corynebacterium glutamicum. looking for any additional role, the function of rama was analyzed in corynebacterium ammoniagenes, which is closely related to c. glutamicum. in this study, we showed that the deltarama mutant constructed by a markerless knockout strategy possessed increased cell surface hydrophobicity, leading to the formation of aggregated cell masses in liquid media. in addition, the mutant exhibited ... | 2010 | 20107993 |
| crystal structures of the multidrug binding repressor corynebacteriumglutamicum cgmr in complex with inducers and with an operator. | cgmr (cgl2612) from corynebacterium glutamicum is a multidrug-resistance-related transcription factor belonging to the tetr family, which is a protein family of widespread bacterial transcription factors typically involved in environmental response. here, we report the crystal structures of cgmr homodimeric repressor in complex with two distinct inducers (1.95 and 1.4 å resolution) and with an operator (2.5 å resolution). the cgmr-operator complex showed that two cgmr dimers bound to the operato ... | 2010 | 20691702 |
| size exclusion chromatography-an improved method to harvest corynebacterium glutamicum cells for the analysis of cytosolic metabolites. | the efficient separation of corynebacterium glutamicum cells from culture medium by size exclusion chromatography (sec) is presented. residue analysis demonstrated that this method effectively depletes extracellular compounds. for evaluation, sec was compared with the common methods cold methanol treatment, fast centrifugation and fast filtration. for this purpose, samples of c. glutamicum cells from fermenter cultures were harvested and subjected to a metabolome analysis. in particular, the wil ... | 2010 | 20817050 |
| corynebacterium glutamicum as an indicator for environmental cobalt and silver stress--a proteome analysis. | cobalt and silver are toxic for cells, but mechanisms of this toxicity are largely unknown. analysis of corynebacterium glutamicum proteome from cells grown in control and cobalt or silver enriched media was performed by two dimensional gel electrophoresis (2de) followed by mass spectrometry. our results indicate that the cell adapted to cobalt stress by inducing five defense mechanisms: scavenging of free radicals, promotion of the generation of energy, reparation of dna, reparation and biogene ... | 2010 | 20818520 |
| the corynebacterium glutamicum aconitase repressor: scratching around for crystals. | imperfections on the surfaces of crystallization containers are known to influence crystal formation and are thought to do so by helping to overcome the nucleation barrier. the intentional creation of imperfections has been widely applied to induce crystallization of small molecules, but has not been reported for protein crystallization. here, the crystallization and preliminary x-ray analysis of the tetr-type aconitase repressor are reported. this regulator was the first transcription factor to ... | 2010 | 20823530 |
| a role of the cspa gene encoding a mycolyltransferase in the growth under alkaline conditions of corynebacterium glutamicum. | corynebacterium glutamicum is widely used in the industrial production of amino acids. producer strains are generated by classical random mutagenesis, and therefore have detrimental characteristics caused by unnecessary mutations. increased alkali sensitivity is one of those undesired characteristics. we found that one of the laboratory strains, aj12036deltacspadeltacspb, showed decreased growth under alkaline conditions. to clarify which mutation is responsible for alkali sensitivity, we constr ... | 2010 | 20699568 |
| stacking multiple transgenes at a selected genomic site via repeated recombinase-mediated dna cassette exchanges. | recombinase-mediated dna cassette exchange (rmce) has been successfully used to insert transgenes at previously characterized genomic sites in plants. following the same strategy, groups of transgenes can be stacked to the same site through multiple rounds of rmce. a gene-silencing cassette, designed to simultaneously silence soybean (glycine max) genes fatty acid ω-6 desaturase 2 (fad2) and acyl-acyl carrier protein thioesterase 2 (fatb) to improve oleic acid content, was first inserted by rmce ... | 2010 | 20720171 |
| antisense-rna-mediated plasmid copy number control in pcg1-family plasmids, pcgr2 and pcg1, in corynebacterium glutamicum. | pcgr2 and pcg1 belong to different subfamilies of the pcg1 family of corynebacterium glutamicum plasmids. nonetheless, they harbour homologous putative antisense rna genes, crri and cgri, respectively. the genes in turn share identical positions complementary to the leader region of their respective repa (encoding plasmid replication initiator) genes. determination of their precise transcriptional start- and end-points revealed the presence of short antisense rna molecules (72 bp, crri; a ... | 2010 | 20798162 |
| towards methionine overproduction in corynebacterium glutamicum--methanethiol and dimethyldisulfide as reduced sulfur sources. | in the present work, methanethiol and dimethyldisulfide were investigated as sulfur source for methionine synthesis in corynebacterium glutamicum. in silico pathway analysis has predicted a high methionine yield for these reduced compounds provided that they can be utilized. wild type cells were able to grow on methanethiol and on dimethyldisulfide as sole sulfur source, respectively. isotope labeling studies with mutant strains exhibiting targeted modification of methionine biosynthesis gave de ... | 2010 | 20798582 |
| biosynthetic pathway for γ-cyclic sarcinaxanthin in micrococcus luteus: heterologous expression and evidence for diverse and multiple catalytic functions of c(50) carotenoid cyclases. | we report the cloning and characterization of the biosynthetic gene cluster (crte, crtb, crti, crte2, crtyg, crtyh, and crtx) of the γ-cyclic c(50) carotenoid sarcinaxanthin in micrococcus luteus nctc2665. expression of the complete and partial gene cluster in escherichia coli hosts revealed that sarcinaxanthin biosynthesis from the precursor molecule farnesyl pyrophosphate (fpp) proceeds via c(40) lycopene, c(45) nonaflavuxanthin, c(50) flavuxanthin, and c(50) sarcinaxanthin. glucosylation of s ... | 2010 | 20802040 |
| link between phosphate starvation and glycogen metabolism in corynebacterium glutamicum, revealed by metabolomics. | in this study, we analyzed the influence of phosphate (p(i)) limitation on the metabolism of corynebacterium glutamicum. metabolite analysis by gas chromatography-time-of-flight (gc-tof) mass spectrometry of cells cultivated in glucose minimal medium revealed a greatly increased maltose level under p(i) limitation. as maltose formation could be linked to glycogen metabolism, the cellular glycogen content was determined. unlike in cells grown under p(i) excess, the glycogen level in p(i)-limited ... | 2010 | 20802079 |
| purification and characterization of the ncgl2923 -encoded 3-hydroxybenzoate 6-hydroxylase from corynebacterium glutamicum. | corynebacterium glutamicum atcc 13032 metabolizes 3-hydroxybenzoate via gentisate. we have now characterized the ncgl2923 -encoded 3-hydroxybenzoate 6-hydroxylase involved in the initial step of 3-hydroxybenzoate catabolism by this strain, a first 3-hydroxybenzoate 6-hydroxylase molecularly and biochemically characterized from a gram-positive strain. the ncg12923 gene from corynebacterium glutamicum atcc 13032 was shown to encode 3-hydroxybenzoate 6-hydroxylase, the enzyme that catalyzes the nad ... | 2010 | 20806251 |
| the zur regulon of corynebacterium glutamicum atcc 13032. | zinc is considered as an essential element for all living organisms, but it can be toxic at large concentrations. bacteria therefore tightly regulate zinc metabolism. the cg2502 protein of corynebacterium glutamicum was a candidate to control zinc metabolism in this species, since it was classified as metalloregulator of the zinc uptake regulator (zur) subgroup of the ferric uptake regulator (fur) family of dna-binding transcription regulators. | 2010 | 20055984 |
| the transcriptional regulators rama and ramb are involved in the regulation of glycogen synthesis in corynebacterium glutamicum. | when grown in glucose-, fructose- or sucrose-containing medium, the amino acid producer corynebacterium glutamicum transiently accumulates large amounts of glycogen (up to 10% of its dry weight), whereas only a marginal amount of glycogen is formed during growth with acetate. this carbon-source-dependent regulation is at least partially due to transcriptional control of glgc, encoding adp-glucose pyrophosphorylase, the first enzyme of glycogen synthesis from glucose-1-phosphate. here, we have an ... | 2010 | 20056699 |
| isolation, evaluation and use of two strong, carbon source-inducible promoters from corynebacterium glutamicum. | to obtain strong, carbon source-inducible promoters useful for industrial applications of corynebacterium glutamicum. | 2010 | 20002569 |
| retention of arsenate using genetically modified coryneform bacteria and determination of arsenic in solid samples by icp-ms. | a novel method for the retention of arsenate [as(v)] combining time-controlled solid-phase extraction with living bacterial biomass is presented. as(v) retention was carried out by exposing the extractant, consisting of a living double-mutant of corynebacterium glutamicum strain arsc1-c2, to the sample for a retention time of 1-7min, before the arsenic distribution equilibrium between the sample solution and the extractant was established. the amount of as(v) retained in the biomass was measured ... | 2010 | 20006108 |
| xylitol production by recombinant corynebacterium glutamicum under oxygen deprivation. | wild-type corynebacterium glutamicum produced 0.6 g l(-1) xylitol from xylose at a productivity of 0.01 g l(-1) h(-1) under oxygen deprivation. to increase this productivity, the pentose transporter gene (arae) from c. glutamicum atcc31831 was integrated into the c. glutamicum r chromosome. consequent disruption of its lactate dehydrogenase gene (ldha), and expression of single-site mutant xylose reductase from candida tenuis (ctxr (k274r)) resulted in recombinant c. glutamicum strain ctxr4 that ... | 2010 | 20012280 |
| analysing overexpression of l-valine biosynthesis genes in pyruvate-dehydrogenase-deficient corynebacterium glutamicum. | l-valine biosynthesis was analysed by comparing different plasmids in pyruvate-dehydrogenase-deficient corynebacterium glutamicum strains in order to achieve an optimal production strain. the plasmids contained different combinations of the genes ilvbncde encoding for the l-valine forming pathway. it was shown that overexpression of the ilvbn genes encoding acetolactate synthase is obligatory for efficient pyruvate conversion and to prevent l-alanine as a by-product. in contrast to earlier studi ... | 2010 | 20012552 |
| importance of nadph supply for improved l-valine formation in corynebacterium glutamicum. | cofactor recycling is known to be crucial for amino acid synthesis. hence, cofactor supply was now analyzed for l-valine to identify new targets for an improvement of production. the central carbon metabolism was analyzed by stoichiometric modeling to estimate the influence of cofactors and to quantify the theoretical yield of l-valine on glucose. three different optimal routes for l-valine biosynthesis were identified by elementary mode (em) analysis. the modes differed mainly in the manner of ... | 2010 | 20014412 |
| impact of adenylyltransferase glne on nitrogen starvation response in corynebacterium glutamicum. | adenylyltransferases regulate glutamine synthetase activity in enterobacteria and actinomycetes such as streptomyces coelicolor, mycobacterium tuberculosis and corynebacterium glutamicum. in this study the effects of a mutation of the glne gene, coding for adenylyltransferase, on transcriptome and metabolome profiles of c. glutamicum was investigated. as expected, the glne deletion led to a loss of activity regulation of glutamine synthetase. astonishingly, additionally the glne mutation caused ... | 2010 | 19963018 |
| the transcriptional regulatory repertoire of corynebacterium glutamicum: reconstruction of the network controlling pathways involved in lysine and glutamate production. | corynebacterium glutamicum is one of the best studied organisms of the high g+c branch of gram-positive bacteria and an emerging model system for the suborder corynebacterineae. to gain insights into the regulatory gene composition and architecture of the transcriptional regulatory network of c. glutamicum, components of the transcriptional regulatory repertoire were intensively studied by many scientific groups in recent years. in this mini-review, we summarize the present knowledge about the d ... | 2010 | 19963020 |
| reconstitution experiments and gene deletions reveal the existence of two-component major cell wall channels in the genus corynebacterium. | two small polypeptides, pora and porh, are known to form cell wall channels in corynebacterium glutamicum and in corynebacterium efficiens. the genes coding for both polypeptides are localized in close proximity to one another between the genes coding for groel2 and a polyphosphate kinase (pkk2). in this study, we investigated the relationship of pora and porh to one another. the results suggested that the major cell wall channels of corynebacterium glutamicum, corynebacterium efficiens, and cor ... | 2010 | 19966008 |
| disulfide bond formation and cysteine exclusion in gram-positive bacteria. | most secretion pathways in bacteria and eukaryotic cells are challenged by the requirement for their substrate proteins to mature after they traverse a membrane barrier and enter a reactive oxidizing environment. for gram-positive bacteria, the mechanisms that protect their exported proteins from misoxidation during their post-translocation maturation are poorly understood. to address this, we separated numerous bacterial species according to their tolerance for oxygen and divided their proteome ... | 2010 | 19940132 |
| adaptation of corynebacterium glutamicum to salt-stress conditions. | corynebacterium glutamicum is one of the biotechnologically most important microorganisms because of its ability to enrich amino acids extracellularly. hence, c. glutamicum requires effective adaptation strategies against both hypo- and hyperosmotic stress. we give a comprehensive and coherent outline about the quantitative dynamics of c. glutamicum during adaptation to hyperosmotic stress at the transcript and protein levels. the osmolyte carrier prop, playing a pivotal role in hyperosmotic str ... | 2010 | 19950167 |
| requirement of de novo synthesis of the odhi protein in penicillin-induced glutamate production by corynebacterium glutamicum. | we found that penicillin-induced glutamate production by corynebacterium glutamicum is inhibited when a de novo protein synthesis inhibitor, chloramphenicol, is added simultaneously with penicillin. when chloramphenicol was added 4 h after penicillin addition, glutamate production was essentially unaffected. (3)h-leucine incorporation experiments revealed that protein synthesis continued for 1 h after penicillin addition and then gradually decreased. these results suggest that de novo protein sy ... | 2010 | 19956942 |
| a novel redox-sensing transcriptional regulator cyer controls expression of an old yellow enzyme family protein in corynebacterium glutamicum. | corynebacterium glutamicum cgr_2930 (cyer) encodes a transcriptional regulator of the arsr family. its gene product, cyer, was shown here to repress the expression of cyer and the cgr_2931 (cye1)-cgr_2932 operon, which is located upstream of cyer in the opposite orientation. the cye1 gene encodes an old yellow enzyme family protein, members of which have been implicated in the oxidative stress response. cyer binds to the intergenic region between cyer and cye1. expression of cyer and cye1 is ind ... | 2010 | 20110293 |
| distribution of aerobic motile and non-motile bacteria within the capillary fringe of silica sand. | retention of bacterial cells as "particles" by silica sand during formation of a capillary fringe (cf) and the influence of motility was examined with motile pseudomonas putida and non-motile corynebacterium glutamicum suspensions in the absence of nutrients. the fractional retention of c. glutamicum cells at all regions of the cf was higher than for p. putida cells, most probably due to the motility of p. putida. only about 5% of p. putida cells and almost no c. glutamicum cells reached the upp ... | 2010 | 20116084 |
| antimalarial and antitubercular nostocarboline and eudistomin derivatives: synthesis, in vitro and in vivo biological evaluation. | the synthesis of nine nostocarboline derivatives with substitutions of the 2-methyl group by alkyl, aryl and functionalized residues, 10 symmetrical bis cationic dimers linking 6-cl-norharmane through the 2-position and fifteen derivatives of the marine alkaloids eudistomin n and o is reported. these compounds were evaluated in vitro against four parasites (trypanosoma brucei rhodesiense stib 900, trypanosoma cruzi tulahuen c2c4, leishmania donovani mhom-et-67/l82 axenic amastigotes, and plasmod ... | 2010 | 20133138 |
| enhancement of ornithine production in proline-supplemented corynebacterium glutamicum by ornithine cyclodeaminase. | in this study, corynebacterium glutamicum and its derived mutants were used to demonstrate the relationship between proline, glutamate and ornithine. the maximum ornithine production was shown in the culture medium (3295.0 mg/l) when the cells were cultured with 20 mm proline and was 15.5 times higher than in the presence of 1 mm proline. however, glutamate, which known as an intermediate in the process of converting proline to ornithine, did not have any positive effect on ornithine production. ... | 2010 | 20134243 |
| approaching the complexity of elastase-digested membrane proteomes using off-gel ief/nlc-maldi-ms/ms. | liquid chromatography, coupled with tandem mass spectrometry, is an established method for the identification of proteins from a complex sample. despite its wide application, the analysis of whole proteomes still represents a challenge to researchers, because of the complexity and dynamic range of protein concentrations in biological samples. the analysis of such samples can be improved by adding a prefractionation step or a combination of orthogonal separation techniques. off-gel isoelectric fo ... | 2010 | 20136094 |
| analysis and engineering of metabolic pathway fluxes in corynebacterium glutamicum. | the gram-positive soil bacterium corynebacterium glutamicum was discovered as a natural overproducer of glutamate about 50 years ago. linked to the steadily increasing economical importance of this microorganism for production of glutamate and other amino acids, the quest for efficient production strains has been an intense area of research during the past few decades. efficient production strains were created by applying classical mutagenesis and selection and especially metabolic engineering s ... | 2010 | 20140657 |
| metabolic changes in a pyruvate kinase gene deletion mutant of corynebacterium glutamicum atcc 13032. | to investigate primary effects of a pyruvate kinase (pyk) defect on glucose metabolism in corynebacterium glutamicum, a pyk-deleted mutant was derived from wild-type c. glutamicum atcc13032 using the double-crossover chromosome replacement technique. the mutant was then evaluated under glutamic acid-producing conditions induced by biotin limitation. the mutant showed an increased specific rate of glucose consumption, decreased growth, higher glutamic acid production, and aspartic acid formation ... | 2010 | 20144730 |
| 13c metabolic flux analysis for larger scale cultivation using gas chromatography-combustion-isotope ratio mass spectrometry. | (13)c-based metabolic flux analysis ((13)cmfa) is limited to smaller scale experiments due to very high costs of labeled substrates. we measured (13)c enrichment in proteinogenic amino acid hydrolyzates using gas chromatography-combustion-isotope ratio mass spectrometry (gc-c-irms) from a series of parallel batch cultivations of corynebacterium glutamicum utilizing mixtures of natural glucose and [1-(13)c] glucose, containing 0%, 0.5%, 1%, 2%, and 10% [1-(13)c] glucose. decreasing the [1-(13)c] ... | 2010 | 20149889 |
| polyphosphate/atp-dependent nad kinase of corynebacterium glutamicum: biochemical properties and impact of ppnk overexpression on lysine production. | nicotinamide adenine dinucleotide phosphate (nadp) is synthesized by phosphorylation of either oxidized or reduced nicotinamide adenine dinucleotide (nad/nadh). here, the cg1601/ppnk gene product from corynebacterium glutamicum genome was purified from recombinant escherichia coli and enzymatic characterization revealed its activity as a polyphosphate (polyp)/atp-dependent nad kinase (ppnk). ppnk from c. glutamicum was shown to be active as homotetramer accepting polyp, atp, and even adp for pho ... | 2010 | 20180116 |
| studies on substrate utilisation in l-valine-producing corynebacterium glutamicum strains deficient in pyruvate dehydrogenase complex. | the pyruvate dehydrogenase complex was deleted to increase precursor availability in corynebacterium glutamicum strains overproducing l: -valine. the resulting auxotrophy is treated by adding acetate in addition glucose for growth, resulting in the puzzling fact of gluconeogenic growth with strongly reduced glucose uptake in the presence of acetate in the medium. this result was proven by intracellular metabolite analysis and labelling experiments. to increase productivity, the sugr protein invo ... | 2010 | 20204663 |
| engineering of sugar metabolism of corynebacterium glutamicum for production of amino acid l-alanine under oxygen deprivation. | corynebacterium glutamicum was genetically engineered to produce l-alanine from sugar under oxygen deprivation. the genes associated with production of organic acids in c. glutamicum were inactivated and the alanine dehydrogenase gene (alad) from lysinibacillus sphaericus was overexpressed to direct carbon flux from organic acids to alanine. although the alad-expressing strain produced alanine from glucose under oxygen deprivation, its productivity was relatively low due to retarded glucose cons ... | 2010 | 20217078 |
| replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring. | high-throughput cultivations in microtiter plates are the method of choice to express proteins from recombinant clone libraries. such processes typically include several steps, whereby some of them are linked by replication steps: transformation, plating, colony picking, preculture, main culture and induction. in this study, the effects of conventional replication methods and replication tools (8-channel pipette, 96-pin replicators: steel replicator with fixed or spring-loaded pins, plastic repl ... | 2010 | 20233443 |
| genetic and functional analysis of the soluble oxaloacetate decarboxylase from corynebacterium glutamicum. | soluble, divalent cation-dependent oxaloacetate decarboxylases (odx) catalyze the irreversible decarboxylation of oxaloacetate to pyruvate and co(2). although these enzymes have been characterized in different microorganisms, the genes that encode them have not been identified, and their functions have been only poorly analyzed so far. in this study, we purified a soluble odx from wild-type c. glutamicum about 65-fold and used matrix-assisted laser desorption ionization-time of flight (maldi-tof ... | 2010 | 20233922 |
| transcriptional regulation of histidine biosynthesis genes in corynebacterium glutamicum. | corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hiseg and hisdcb-orf1-orf2-hisha-impa-hisfi. the latter his operon starts the transcription at the c residue localized 196 bp upstream of the hisd atg start codon. our computer-based sequence analysis showed that the region corresponding to the untranslated 5' end of the transcript, named the hisd ... | 2010 | 20237580 |
| the fha domain of odhi interacts with the carboxyterminal 2-oxoglutarate dehydrogenase domain of odha in corynebacterium glutamicum. | in corynebacterium glutamicum, the unphosphorylated 15-kda odhi protein inhibits the activity of the 2-oxoglutarate dehydrogenase complex (odhc) by binding to odha, which in corynebacteria and mycobacteria is a large fusion protein with two major domains exhibiting structural features of e1o and e2 proteins. using copurification and surface plasmon resonance experiments with different odhi and odha length variants it was shown that the entire forkhead-associated (fha) domain of odhi and the c-te ... | 2010 | 20303957 |
| carbohydrate metabolism in corynebacterium glutamicum and applications for the metabolic engineering of l-lysine production strains. | carbohydrates exclusively serve as feedstock for industrial amino acid production with corynebacterium glutamicum. due to the industrial interest, knowledge about the pathways for carbohydrate metabolization in c. glutamicum steadily increases, enabling the rational design of optimized strains and production processes. in this review, we provide an overview of the metabolic pathways for utilization of hexoses (glucose, fructose), disaccharides (sucrose, maltose), pentoses (d-ribose, l-arabinose, ... | 2010 | 20333512 |
| a deficiency in arabinogalactan biosynthesis affects corynebacterium glutamicum mycolate outer membrane stability. | corynebacterineae is a specific suborder of gram-positive bacteria that includes mycobacterium tuberculosis and corynebacterium glutamicum. the ultrastructure of the cell envelope is very atypical. it is composed of a heteropolymer of peptidoglycan and arabinogalactan (ag) covalently associated to an outer membrane. five arabinosyltransferases are involved in the biosynthesis of ag in c. glutamicum. aftb catalyzes the transfer of araf (arabinofuranosyl) onto the arabinan domain of the arabinogal ... | 2010 | 20363942 |
| factors enhancing l-valine production by the growth-limited l-isoleucine auxotrophic strain corynebacterium glutamicum deltailva deltapanb ilvnm13 (peckailvbnc). | cell growth limitation is known to be an important condition that enhances l: -valine synthesis in corynebacterium glutamicum recombinant strains with l: -isoleucine auxotrophy. to identify whether it is the limited availability of l: -isoleucine itself or the l: -isoleucine limitation-induced rel-dependent ppgpp-mediated stringent response that is essential for the enhancement of l: -valine synthesis in growth-limited c. glutamicum cells, we deleted the rel gene, thereby constructing a relaxed ... | 2010 | 20364396 |
| engineering corynebacterium glutamicum for isobutanol production. | the production of isobutanol in microorganisms has recently been achieved by harnessing the highly active 2-keto acid pathways. since these 2-keto acids are precursors of amino acids, we aimed to construct an isobutanol production platform in corynebacterium glutamicum, a well-known amino-acid-producing microorganism. analysis of this host's sensitivity to isobutanol toxicity revealed that c. glutamicum shows an increased tolerance to isobutanol relative to escherichia coli. overexpression of al ... | 2010 | 20376637 |
| functional characterization of the glxr deletion mutant of corynebacterium glutamicum atcc 13032: involvement of glxr in acetate metabolism and carbon catabolite repression. | recently, a cyclic amp receptor protein homologue, glxr, was reported to bind to the upstream regions of several genes involved in the regulation of diverse physiological processes in corynebacterium glutamicum. however, the function of glxr has not yet been explored in c. glutamicum in vivo using a glxr deletion mutant. therefore, this study examines the role of glxr as a repressor in glyoxylate bypass and carbon catabolite repression (ccr) using a deletion mutant. the disruption of glxr result ... | 2010 | 20377641 |
| cg2091 encodes a polyphosphate/atp-dependent glucokinase of corynebacterium glutamicum. | the corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (polyp)/atp-dependent glucokinase (ppgk). previous work demonstrated the association of ppgk to polyp granules. the deduced amino acid sequence of ppgk shows 45% sequence identity to polyp/atp glucomannokinase of arthrobacter sp. strain km and 50% sequence identity to polyp glucokinase of mycobacterium tuberculosis h37rv. ppgk from c. glutamicum was purified from recombinant escherichia coli. polyp was highly preferred over a ... | 2010 | 20379711 |
| systems-wide metabolic pathway engineering in corynebacterium glutamicum for bio-based production of diaminopentane. | in the present work the gram-positive bacterium corynebacterium glutamicum was engineered into an efficient, tailor-made production strain for diaminopentane (cadaverine), a highly attractive building block for bio-based polyamides. the engineering comprised expression of lysine decarboxylase (ldcc) from escherichia coli, catalyzing the conversion of lysine into diaminopentane, and systems-wide metabolic engineering of central supporting pathways. substantially re-designing the metabolism yielde ... | 2010 | 20381632 |
| decolorization and biodegradation of reactive dyes and dye wastewater by a developed bacterial consortium. | a bacterial consortium (consortium gr) consisting of proteus vulgaris ncim-2027 and micrococcus glutamicus ncim-2168 could rapidly decolorize and degrade commonly-used sulfonated reactive dye green he4bd and many other reactive dyes. consortium gr shows markedly higher decolorization activity than that of the individual strains. the preferable physicochemical parameters were identified to achieve higher dye degradation and decolorization efficiency. the supplementation of cheap co-substrates (e. ... | 2010 | 20407917 |
| udp-(5f)-glcnac acts as a slow-binding inhibitor of msha, a retaining glycosyltransferase. | glycosyltransferase enzymes play important roles in numerous cellular pathways. despite their participation in many therapeutically relevant pathways, there is a paucity of information on how to effectively inhibit this class of enzymes. here we report that udp-(5f)-glcnac acts as a slow-binding, competitive inhibitor of the retaining glycosyltransferase msha from corynebacterium glutamicum (k(i) approximately 1.6 mum). the kinetic data are consistent with a single-step inhibition mechanism whos ... | 2010 | 20411981 |
| subcellular localization and characterization of the parab system from corynebacterium glutamicum. | faithful segregation of chromosomes and plasmids is a vital prerequisite to produce viable and genetically identical progeny. bacteria use a specialized segregation system composed of the partitioning proteins para and parb to segregate certain plasmids. strikingly, homologues of para and parb are found to be encoded in many chromosomes. although mutations in the chromosomal par system have effects on segregation efficiency, the exact mechanism by which the chromosomes are segregated into the da ... | 2010 | 20435732 |
| utilization of phenol and naphthalene affects synthesis of various amino acids in corynebacterium glutamicum. | this article reports multiple metabolic pathways of amino acid production via phenol and naphthalene use by corynebacterium glutamicum. biodegradation of phenol and naphthalene by c. glutamicum occurred in a mineral salt medium containing 1% yeast extract without any additional carbon sources. among the amino acids synthesized via the tca-cycle, glutamate synthesis increased in c. glutamicum supplemented with 8.5 mm phenol or with 4.2 mm naphthalene. aspartate synthesis significantly increased w ... | 2010 | 20443004 |
| codon usage patterns in corynebacterium glutamicum: mutational bias, natural selection and amino acid conservation. | the alternative synonymous codons in corynebacterium glutamicum, a well-known bacterium used in industry for the production of amino acid, have been investigated by multivariate analysis. as c. glutamicum is a gc-rich organism, g and c are expected to predominate at the third position of codons. indeed, overall codon usage analyses have indicated that c and/or g ending codons are predominant in this organism. through multivariate statistical analysis, apart from mutational selection, we identifi ... | 2010 | 20445740 |
| odhi dephosphorylation kinetics during different glutamate production processes involving corynebacterium glutamicum. | in corynebacterium glutamicum, the activity of the 2-oxoglutarate dehydrogenase complex was shown to be controlled by the phosphorylation of a 15-kda protein odhi by different serine/threonine protein kinases. in this paper, the phosphorylation status and kinetics of odhi dephosphorylation were assessed during glutamate producing processes triggered by either a biotin limitation or a temperature upshock from 33 degrees c to 39 degrees c. a dephosphorylation of odhi in c. glutamicum 2262 was obse ... | 2010 | 20449744 |
| transcriptional regulation of gene expression in corynebacterium glutamicum: the role of global, master and local regulators in the modular and hierarchical gene regulatory network. | the genus corynebacterium belongs to the taxonomic class actinobacteria representing the high g+c branch of gram-positive bacteria. among the most prominent members of this genus is corynebacterium glutamicum, which is used by the biotechnological industry for the fermentative production of l-amino acids. because of its industrial importance, c. glutamicum is one of the best-studied gram-positive bacteria and an emerging model for the suborder corynebacterineae. over the past years, the transcri ... | 2010 | 20491930 |
| o-mycoloylated proteins from corynebacterium: an unprecedented post-translational modification in bacteria. | o-acylation of proteins was known only in a few eukaryotic proteins but never in bacteria. we demonstrate, using a combination of protein chemistry and mass spectrometry, the occurrence of three o-acylated polypeptides in corynebacterium glutamicum, pora, porh, and an unknown small protein. the three polypeptides are o-substituted by mycolic acids, long chain alpha-alkyl and beta-hydroxy fatty acids specifically produced by members of the corynebacterineae suborder. to date these acids were desc ... | 2010 | 20508265 |
| identification of a suppressor gene for the arginine-auxotrophic argj mutation in corynebacterium glutamicum. | we recently proposed a metabolic engineering strategy for l-ornithine production based on the hypothesis that an increased intracellular supply of n-acetylglutamate may further enhance l-ornithine production in a well-defined recombinant strain of corynebacterium glutamicum. in this work, an argj-deficient arginine auxotrophic mutant of c. glutamicum is suppressed by a different locus of c. glutamicum atcc13032. overexpression of the ncgl1469 open reading frame (orf), exhibiting n-acetylglutamat ... | 2010 | 20544254 |
| characterization of a 24-kb plasmid pcgr2 newly isolated from corynebacterium glutamicum. | a 24-kb plasmid with 21 open reading frames (orfs) was newly isolated from corynebacterium glutamicum atcc 14997 and named pcgr2. three of its orfs were indispensable for stable autonomous replication of pcgr2 in c. glutamicum: in the absence of selective pressure, deletion derivatives of pcgr2 containing the three orfs showed stability in c. glutamicum for over 50 generations. the first of these orfs encoded replicase repa whose gene product revealed high amino acid sequence similarity to corre ... | 2010 | 20552356 |
| identification and elimination of the competing n-acetyldiaminopentane pathway for improved production of diaminopentane by corynebacterium glutamicum. | the present work describes the development of a superior strain of corynebacterium glutamicum for diaminopentane (cadaverine) production aimed at the identification and deletion of the underlying unknown n-acetyldiaminopentane pathway. this acetylated product variant, recently discovered, is a highly undesired by-product with respect to carbon yield and product purity. initial studies with c. glutamicum dap-3c, a previously derived tailor-made diaminopentane producer, showed that up to 20% of th ... | 2010 | 20562290 |
| mechanism of concerted inhibition of alpha2beta2-type hetero-oligomeric aspartate kinase from corynebacterium glutamicum. | aspartate kinase (ak) is the first and committed enzyme of the biosynthetic pathway producing aspartate family amino acids, lysine, threonine, and methionine. ak from corynebacterium glutamicum (cgak), a bacterium used for industrial fermentation of amino acids, including glutamate and lysine, is inhibited by lysine and threonine in a concerted manner. to elucidate the mechanism of this unique regulation in cgak, we determined the crystal structures in several forms: an inhibitory form complexed ... | 2010 | 20573952 |
| an improved shuttle vector constructed for metabolic engineering research in corynebacterium glutamicum. | corynebacterium glutamicum is an industrial microorganism for production of amino acids. however, the metabolic engineering in c. glutamicum has been retarded due to lack of suitable vectors. in this study, we have constructed a shuttle vector pdxw-10 which harbors a large multiple cloning site suitable for cloning multiple genes, and a tac-m promoter suitable for constitutive gene expression in c. glutamicum. the cat gene was subcloned into the vector and the expression levels of the cat protei ... | 2010 | 20580910 |
| fed-batch production of a bioflocculant from corynebacterium glutamicum. | the constant-rate fed-batch production of the polygalacturonic acid bioflocculant rea-11 was studied. a controlled sucrose-feeding strategy resulted in a slight improvement in biomass and a 7% reduction in flocculating activity compared with the batch process. when fed with a 3 g l(-1) urea solution, the flocculating activity was enhanced to 720 u ml(-1) in 36 h. high cell density (2.12 g l(-1)) and flocculating activity (820 u ml(-1)) were obtained in a 10-l fermentor by feeding with a sucrose- ... | 2010 | 20589412 |
| biochemical characterization of two thymidylate synthases in corynebacterium glutamicum nchu 87078. | the genome of corynebacterium glutamicum nchu 87078 contains two putative thymidylate synthase genes, designated cgthya and cgthyx. these two genes were expressed in escherichia coli novablue and the expressed his(6)-tagged enzymes were purified by nickel-chelate chromatography. the purified cgthya had a specific activity of 414 mu mg(-)(1) protein, whereas thymidylate synthase activity for cgthyx could not be detected in a functional complementation assay using a 10-day incubation period. gel f ... | 2010 | 20595007 |
| the properties and contribution of the corynebacterium glutamicum mscs variant to fine-tuning of osmotic adaptation. | based on sequence similarity, the msccg gene product of corynebacterium glutamicum belongs to the family of mscs-type mechanosensitive channels. in order to investigate the physiological significance of msccg in response to osmotic shifts in detail, we studied its properties using both patch-clamp techniques and betaine efflux kinetics. after heterologous expression in an escherichiacoli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant e. coli spheroplasts msccg showe ... | 2010 | 20599688 |
| regulation of the expression of genes involved in nad de novo biosynthesis in corynebacterium glutamicum. | three genes, nada, nadb, and nadc, involved in nad de novo biosynthesis are broadly conserved in the genomes of numerous bacterial species. in the genome of corynebacterium glutamicum, nada and nadc but not nadb are annotated. the nada and nadc genes are located in a gene cluster containing two other genes, designated ndnr and nads herein. ndnr encodes a member of the nudix-related transcriptional regulator (nrtr) family. nads encodes a homologue of cysteine desulfurase involved in fe-s cluster ... | 2010 | 20601509 |
| rama and ramb are global transcriptional regulators in corynebacterium glutamicum and control genes for enzymes of the central metabolism. | in corynebacterium glutamicum, the transcriptional regulators of acetate metabolism rama (encoded by cg2831) and ramb (encoded by cg0444) play an important role in expression control of genes involved in acetate and ethanol metabolism. both regulators were speculated to have broader significance in expression control of further genes in the central metabolism of c. glutamicum. here we investigated the rama and ramb regulons by genome-wide transcriptome analysis with special emphasis on genes enc ... | 2010 | 20620178 |
| phosphorylation of a novel cytoskeletal protein (rsmp) regulates rod-shaped morphology in corynebacterium glutamicum. | corynebacteria grow by wall extension at the cell poles, with diviva being an essential protein orchestrating cell elongation and morphogenesis. diviva is considered a scaffolding protein able to recruit other proteins and enzymes involved in polar peptidoglycan biosynthesis. partial depletion of diviva induced overexpression of cg3264, a previously uncharacterized gene that encodes a novel coiled coil-rich protein specific for corynebacteria and a few other actinomycetes. by partial depletion a ... | 2010 | 20622015 |
| citrate synthase in corynebacterium glutamicum is encoded by two glta transcripts which are controlled by rama, ramb, and glxr. | citrate synthase (cs) is located at a major branch point in the metabolism and is required for both tricarboxylic acid and glyoxylic acid cycle activity. here we show that the cs gene glta of corynebacterium glutamicum is monocistronic, but that two transcripts are formed with their transcript initiation sites located 121bp and 357bp upstream of the translational start codon, respectively. northern blot analyses revealed that during growth on acetate the short transcript prevails, whereas during ... | 2010 | 20630483 |
| recovery of zero-valent gold from cyanide solution by a combined method of biosorption and incineration. | a new combined way of biosorption and incineration is presented for the recovery of gold from gold-cyanide solutions. decarboxylated biosorbent (dcb) was prepared by removing interfering carboxyl groups from the surface of inactive corynebacterium glutamicum. the recovery of gold from the exhausted biosorbents was performed using elution or incineration. the maximum gold(i) uptakes were obtained as 50.19 and 86.16mg/g for the raw biomass and dcb, respectively. the biosorption performance of dcb ... | 2010 | 20630746 |
| alternative thymidylate synthase, thyx, involved in corynebacterium glutamicum atcc 13032 survival during stationary growth phase. | a blastp search has shown the presence of a gene homologous to an alternative thymidylate synthase (ts), thyx, in corynebacterium glutamicum atcc 13032. to determine if thyx is functionally analogous to thya, thyx was cloned in a plasmid and the resulting construct was transferred by transformation into a thya mutant of escherichia coli. the thyx from c. glutamicum compensated for the defect in ts-deficient e. coli. a functional knockout of the thyx gene was constructed by allelic replacement us ... | 2010 | 20636973 |
| treatment of phenol-contaminated soil by corynebacterium glutamicum and toxicity removal evaluation. | biodegradation of phenol-contaminated soils using corynebacterium glutamicum, was optimized in this study. phenol degradation by c. glutamicum was observed in soil supplemented with 1% yeast extract as a substrate. we determined the optimal inoculation size of c. glutamicum (7.4 log(10) cfu ml(-1)) for the degradation of phenol-contaminated soil. under optimal conditions (1% yeast extract and 7.4 log(10) cfu ml(-1) of c. glutamicum), the efficiency of phenol degradation in soil was greater than ... | 2010 | 20638173 |
| production of the amino acids l-glutamate, l-lysine, l-ornithine and l-arginine from arabinose by recombinant corynebacterium glutamicum. | amino acid production processes with corynebacterium glutamicum are based on media containing glucose from starch hydrolysis or fructose and sucrose as present in molasses. simultaneous utilization of various carbon sources, including glucose, fructose and sucrose, in blends is a typical characteristic of this bacterium. the renewable non-food carbon source arabinose, which is present in hemicellulosic hydrolysates, cannot be utilized by most c. glutamicum strains. heterologous expression of the ... | 2010 | 20638422 |
| the pstscab operon for phosphate uptake is regulated by the global regulator glxr in corynebacterium glutamicum. | the pstscab operon of corynebacterium glutamicum, which encodes a high affinity transport system for uptake of the phosphorus source inorganic phosphate, is induced upon phosphate starvation involving activation by the two-component regulatory system phos-phor. partial phosphate starvation induction of the pstscab operon in a deltaphors mutant indicated the involvement of (an) additional transcriptional regulator(s). here, glxr, a global camp-dependent transcriptional regulator, was shown to bin ... | 2010 | 20638427 |
| stationary versus non-stationary (13)c-mfa: a comparison using a consistent dataset. | besides the well-established (13)c-metabolic flux analysis ((13)c-mfa) which characterizes a cell's fluxome in a metabolic and isotopic stationary state a current area of research is isotopically non-stationary mfa. non-stationary (13)c-mfa uses short-time isotopic transient data instead of long-time isotopic equilibrium data and thus is capable to resolve fluxes within much shorter labeling experiments. however, a comparison of both methods with data from one single experiment has not been made ... | 2010 | 20638432 |
| rosr (cg1324), a hydrogen peroxide-sensitive marr-type transcriptional regulator of corynebacterium glutamicum. | the cg1324 gene (rosr) of corynebacterium glutamicum encodes a marr-type transcriptional regulator. by a comparative transcriptome analysis with dna microarrays of a δrosr mutant and the wild type and subsequent emsas with purified rosr protein, direct target genes of rosr were identified. the narkghji operon, which encodes a nitrate/nitrite transporter and the dissimilatory nitrate reductase complex, was activated by rosr. all other target genes were repressed by rosr. they encode four putative ... | 2010 | 20643656 |