Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of vibrio vulnificus isolated from cockles (anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic dna analysis. | antibiotic susceptibility, plasmid profiles and random amplification of polymorphic dna (rapd) were used to study strains of vibrio vulnificus isolated from cockles (anadara granosa). thirty-six isolates were analyzed. the prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). among these, 21 strains of biotype 1 and two strains of biotype 2 contained plasmid dna bands ranging in size from 1.4 to 9.7 mda. thirty-one (83.3%) were found to be resistant to one or more of the antimicrobial ... | 1998 | 9711850 |
| structure of a muramic acid containing capsular polysaccharide from the pathogenic strain of vibrio vulnificus atcc 27562. | vibrio vulnificus strains isolated from septicemia cases and from the environment show a wide variety of capsular types. in an attempt to find common structural features which can be correlated with pathogenicity and toxicity, we have determined structures of the capsular polysaccharides (cps) from several pathogenic strains. we report the complete structure of the polysaccharide from the pathogenic v. vulnificus strain atcc 27562 using a combination of homonuclear and heteronuclear one-dimensio ... | 1998 | 9720237 |
| minimum inhibitory concentration of smoke wood extracts against spoilage and pathogenic micro-organisms associated with foods. | antimicrobial activity of seven commercial smoke preparations (four liquid and three solid) was studied. the minimum inhibitory concentration (mic) was determined against a selection of food spoilage and pathogenic micro-organisms. the main smoke components were identified and quantified by gas chromatography/mass spectrometry. the most effective condensate was s2. all strains except salmonella enteritidis were inhibited by s2 with an mic < 0.5-1.5%. smoke extract l2 inhibited growth of vibrio v ... | 1998 | 9722997 |
| genetic relatedness among environmental, clinical, and diseased-eel vibrio vulnificus isolates from different geographic regions by ribotyping and randomly amplified polymorphic dna pcr. | genetic relationships among 132 strains of vibrio vulnificus (clinical, environmental, and diseased-eel isolates from different geographic origins, as well as seawater and shellfish isolates from the western mediterranean coast, including reference strains) were analyzed by random amplified polymorphic dna (rapd) pcr. results were validated by ribotyping. for ribotyping, dnas were digested with kpni and hybridized with an oligonucleotide probe complementary to a highly conserved sequence in the ... | 1998 | 9726889 |
| the role of gulf coast oysters harvested in warmer months in vibrio vulnificus infections in the united states, 1988-1996. vibrio working group. | vibrio vulnificus infections are highly lethal and associated with consumption of raw shellfish and exposure of wounds to seawater. v. vulnificus infections were reported to the centers for disease control and prevention from 23 states. for primary septicemia infections, oyster trace-backs were performed and water temperature data obtained at harvesting sites. between 1988 and 1996, 422 infections were reported; 45% were wound infections, 43% primary septicemia, 5% gastroenteritis, and 7% from u ... | 1998 | 9728544 |
| direct identification of vibrio vulnificus in clinical specimens by nested pcr. | this study was performed to establish optimal nested pcr conditions and a high-yield dna extraction method for the direct identification of vibrio vulnificus in clinical specimens. we designed two sets of primers targeting the v. vulnificus hemolysin/cytolysin gene. the target of the first primer set (p1-p2; sense, 5'-gac-tat-cgc-atc-aac-aac-cg-3', and antisense, 5'-agg-tag-cga-gta-tta-ctg-cc-3', respectively) is a 704-bp dna fragment. the second set (p3-p4; sense, 5'-gct-att-tca-ccg-ccg-ctc-ac- ... | 1998 | 9738039 |
| a comparison of strategies for the detection and recovery of vibrio vulnificus from marine samples of the western mediterranean coast. | we have compared the effectiveness of culture-based methods and a dna-based method for the detection, of vibrio vulnificus from a seawater and three types of shellfish collected from the costal waters of valencia, spain. for culture-based method, we used two selective media, thiosulphate-citrate-salts-sucrose (tcbs), and cellobiose-polymyxin b-colistin (cpc) agars with and without previous enrichment in alkaline-saline-peptone-water (apws). presumptive colonies were confirmed as v. vulnificus by ... | 1998 | 9741117 |
| characterization of the hemorrhagic reaction caused by vibrio vulnificus metalloprotease, a member of the thermolysin family. | vibrio vulnificus is an opportunistic human pathogen causing wound infections and septicemia, characterized by hemorrhagic and edematous damage to the skin. this human pathogen secretes a metalloprotease (v. vulnificus protease [vvp]) as an important virulence determinant. when several bacterial metalloproteases including vvp were injected intradermally into dorsal skin, vvp showed the greatest hemorrhagic activity. the level of the in vivo hemorrhagic activity of the bacterial metalloproteases ... | 1998 | 9746589 |
| the use of alkaline phosphatase-labelled oligonucleotide probes as culture confirmation reagents for identification of commercially important bacteria. | a range of rrna-targeted alkaline phosphatase labelled oligonucleotide probes was tested for use as culture confirmation reagents for the rapid identification of micro-organisms. the probes were specific to clinically important bacteria (helicobacter pylori and mycobacterium tuberculosis), fish and shellfish pathogens (renibacterium salmoninarum and vibrio vulnificus), food spoilage bacteria (listeria spp. and l. monocytogenes), for bacteria of biotechnological importance (streptomyces spp.) and ... | 1998 | 9750334 |
| occurrence and distribution of vibrio vulnificus in tropical fish and shellfish from cochin (india). | the incidence and distribution of vibrio vulnificus in marine and brackish-water fish and shellfish from coastal areas of cochin on the west coast of india were studied. for marine fish collected in very fresh condition from vessels, a level of incidence of 16.6% was noted. frequency of isolation was greater from the intestine than from the muscle. the greater most probable number (mpn) count determined by the three tube method ranged from 15 to 910 g-1 in the positive samples. | 1998 | 9569691 |
| improved isolation of vibrio vulnificus from seawater and sediment with cellobiose-colistin agar. | an improved selective medium, cellobiose-colistin (cc) agar, gave a significantly higher (p < 0.05) isolation rate of vibrio vulnificus from water and sediment samples than did modified cellobiose-polymyxin b-colistin (mcpc) agar. in a total of 446 alkaline peptone water preenrichments amended with polymyxin b, v. vulnificus was isolated from 154 preenrichments (35%) with mcpc agar and from 179 preenrichments (40%) with cc agar. cc agar gave a higher plating efficiency of v. vulnificus cells tha ... | 1998 | 9572942 |
| an epimerase gene essential for capsule synthesis in vibrio vulnificus. | the extracellular capsule polysaccharide (cps) of vibrio vulnificus is a primary virulence factor which allows survival of the bacteria in the human host. to study the genes involved in expression of the capsule, we generated mutants that lost the ability to produce cps following the insertion of a minitransposon into the genome of an encapsulated, clinical strain of v. vulnificus. a genomic region, from one nonencapsulated mutant, containing the transposon and flanking v. vulnificus dna was clo ... | 1998 | 9596722 |
| minocycline and cefotaxime in the treatment of experimental murine vibrio vulnificus infection. | we conducted an in vivo study with the mouse model of vibrio vulnificus infection to evaluate the efficacies of therapy with minocycline or cefotaxime alone and in combination. v. vulnificus was introduced subcutaneously into the area over the right thigh. the inoculum size ranged from 1.0 x 10(3) to 1.2 x 10(8) cfu from experiment to experiment but was constant for all animals in the same experiment. antibiotics were given intraperitoneally 2 h after the bacteria were inoculated. in experiments ... | 1998 | 9624467 |
| [recovery from septicemia caused by fecally identified vibrio vulnificus and complicated by ulcerative colitis ]. | 1998 | 9627485 | |
| vibrio vulnificus septicemia in a patient with severe aplastic anemia. | a 53-year-old man with severe aplastic anemia developed sporadic vibrio vulnificus septicemia 1 day after eating raw fish and shellfish. although v. vulnificus infection is potentially fatal, he was saved by immediate and sensitive antibiotic administration. patients with chronic hematologic disease are susceptible to infection by this organism and are prone to developing septicemia when they eat raw seafood. it is necessary for a patient with this infection to be given effective antibiotics as ... | 1998 | 9631584 |
| cloning and characterization of an outer membrane protein of vibrio vulnificus required for heme utilization: regulation of expression and determination of the gene sequence. | vibrio vulnificus is a halophilic, marine pathogen that has been associated with septicemia and serious wound infections in patients with iron overload and preexisting liver disease. for v. vulnificus, the ability to acquire iron from the host has been shown to correlate with virulence. v. vulnificus is able to use host iron sources such as hemoglobin and heme. we previously constructed a fur mutant of v. vulnificus which constitutively expresses at least two iron-regulated outer membrane protei ... | 1998 | 9632577 |
| enhanced broth media for selective growth of vibrio vulnificus. | rapid detection of vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. pnc (5% peptone, 1% nacl, and 0.08% cellobiose [ph 8.0]) enhanced the growth of v. vulnificus compared to alkaline peptone broth. pncc (pnc with 1.0 to 4.1 u of colistin methanesulfonate per ml) increased the growth of low levels of v. vulnificus while suppressing non-target bacteria. | 1998 | 9647852 |
| [vibrio vulnificus infections]. | 1998 | 9648448 | |
| necrotizing fasciitis caused by vibrio vulnificus differs from that caused by streptococcal infection. | we reviewed the clinical record of all patients admitted to saga medical school hospital during the most recent 10 years and found that 17 (0.03%) were diagnosed as having necrotizing fasciitis. bacteriological examination demonstrated that vibrio vulnificus was the pathogen responsible in five patients (29%). the disease caused by v. vulnificus occurred in the warmer half of the year. all of the patients had underlying chronic liver dysfunction, and three of them had previously consumed raw sea ... | 1998 | 9661943 |
| vibrio vulnificus sepsis associated with coincidental diagnosis of acute myeloid leukemia. | 1998 | 9662287 | |
| detection of microbial pathogens in shellfish with multiplex pcr. | multiplex pcr amplification of uida, cth, inva, ctx, and tl genes was developed enabling simultaneous detection in shellfish of escherichia coli, an indicator of fecal contamination and microbial pathogens, salmonella typhimurium, vibrio vulnificus, v. cholerae, and v. parahaemolyticus, respectively. each of the five pairs of oligonucleotide primers was found to support pcr amplifications of only its targeted gene. the optimized multiplex pcr reaction utilized a pcr reaction buffer containing 2. ... | 1998 | 9662609 |
| identification of the vibrio cholerae type 4 prepilin peptidase required for cholera toxin secretion and pilus formation. | cholera toxin secretion is dependent upon the extracellular protein secretion apparatus encoded by the eps gene locus of vibrio cholerae. although the eps gene locus encodes several type four prepilin-like proteins, the peptidase responsible for processing these proteins has not been identified. this report describes the identification of a prepilin peptidase from the v. cholerae genomic database by virtue of its homology with the pild prepilin peptidase of pseudomonas aeruginosa. plasmid disrup ... | 1998 | 9781884 |
| raw shellfish consumption among renal disease patients. a risk factor for severe vibrio vulnificus infection. | raw shellfish-associated vibrio vulnificus septicemia, with a case-fatality rate of nearly 50%, occurs most commonly in immunocompromised patients or those with liver disease. | 1998 | 9791643 |
| infections due to non-o1 vibrio cholerae in southern taiwan: predominance in cirrhotic patients. | although taiwan is not an area where cholera is endemic, from october 1988 to october 1997 30 episodes of non-o1, non-o139 vibrio cholerae infection were noted at the national cheng kung university hospital in taiwan. infections generally occurred in hot seasons, and two episodes were concomitant with vibrio vulnificus infection. three major clinical presentations were found: bacteremia with concurrent spontaneous bacterial peritonitis or invasive soft-tissue infections that occurred solely in c ... | 1998 | 9798033 |
| the type iv leader peptidase/n-methyltransferase of vibrio vulnificus controls factors required for adherence to hep-2 cells and virulence in iron-overloaded mice. | vibrio vulnificus expresses a number of potential virulence determinants that may contribute to its ability to cause a severe and rapidly disseminating septicemia in susceptible hosts. we have cloned and characterized two genes encoding products related to components of the type iv pilus biogenesis and general secretory (type ii) pathways by complementation of a type iv peptidase/n-methyltransferase (pild) mutant of pseudomonas aeruginosa with a v. vulnificus genomic library. one of the genes (v ... | 1998 | 9826339 |
| heterogeneity among isolates of vibrio vulnificus recovered from eels (anguilla anguilla) in denmark. | the findings of this study demonstrate that vibrio vulnificus isolates recovered from diseased eels in denmark are heterogeneous as shown by o serovars, capsule types, ribotyping, phage typing, and plasmid profiling. the study includes 85 v. vulnificus isolates isolated from the gills, intestinal contents, mucus, spleen, and kidneys of eels during five disease outbreaks on two danish eel farms from 1995 to 1997, along with a collection of 12 v. vulnificus reference strains. the results showed th ... | 1998 | 9835548 |
| necrotizing fasciitis caused by vibrio vulnificus: first published infection acquired in turkey is the second time a strain is isolated in germany. | vibrio vulnificus, a marine vibrio, has recently been recognized as a potential human pathogen. it causes human infections with mortality rates up to 60%. until 1991, most human isolations were reported from the usa, japan and taiwan. the second strain isolated in germany is documented and a significant case of v. vulnificus infection acquired in turkey is published for the first time. | 1998 | 9861568 |
| hand infections due to non-cholera vibrio after injuries from st peter's fish (tilapia zillii). | we report 49 patients with a wide variety of hand infections, which developed after injuries from st peter's fish (tilapia zillii). twenty-eight of 36 patients who had been operated on had non-cholera vibrio infections, all identified as vibrio vulnificus. the course in these patients was characterized by rapid spread of the infection with progressive necrosis of the tendon sheath, subcutaneous tissues and the skin. two of them required amputations but the others had satisfactory functional resu ... | 1998 | 9888689 |
| cytotoxicity of vibrio vulnificus cytolysin on rat peritoneal mast cells. | histamine has been thought to be a permeability enhancing factor in vibrio vulnificus infection. the injection of living bacteria or purified v. vulnificus cytolysin (vvc) can cause lethality in mice by inducing hemoconcentration and increased vascular permeability. in the present study, we tried to identify whether histamine release causes the increased vascular permeability that is responsible for the lethal effect of vvc. treatment of rat peritoneal mast cells with high concentrations of vvc ... | 1998 | 10037218 |
| fatal sepsis from vibrio vulnificus in a hemodialyzed patient. | vibrio vulnificus, a particularly virulent halophilic vibrio, has been isolated from the blood and skin necrotic lesion of a hemodialyzed patient with sepsis. the patient has had exposure of the skin to seawater. various chronic conditions including renal failure have a great risk for developing septicemia due to v vulnificus. it is necessary to inform persons with liver diseases or immunocompromising conditions of hazards associated with the consumption of undercooked seafood and seawater expos ... | 1998 | 9496743 |
| involvement of bradykinin generation in intravascular dissemination of vibrio vulnificus and prevention of invasion by a bradykinin antagonist. | involvement of bradykinin generation in bacterial invasion was examined by using a gram-negative bacillus, vibrio vulnificus, which is known to invade the blood circulatory system and cause septicemia. v. vulnificus was injected intraperitoneally (i.p.) into mice with or without bradykinin or a bradykinin (b2 receptor) antagonist. dissemination of v. vulnificus from peritoneal septic foci to the circulating blood was assessed by counting of viable bacteria in venous blood by use of the colony-fo ... | 1998 | 9453658 |
| clinical infections of vibrio vulnificus: a case report and review of the literature. | vibrio vulnificus is a marine gram-negative bacillus that is recognized as a cause of fulminant primary septicemia and wound infections. one of the most common bacteria in seawater, v. vulnificus is concentrated in ocean filter feeders (e.g., oysters and clams). primary septicemia can occur in patients, typically with underlying liver disease, who have acquired the organism through the gastrointestinal tract after recent consumption of raw shellfish. characterized by fevers, chills, and bullous ... | 1998 | 9472762 |
| [vibrio vulnificus septicemia in spain]. | vibrio vulnificus is a virulent marine organism, able to contaminate sea-food. it usually produces bacteremia associated with secondary skin lesions in patients with underlying conditions, such as hepatic cirrhosis. we report a case of septic shock and characteristic skin lesions, due to vibrio vulnificus in a patient with cirrhosis, who had eaten raw oysters. the patient survived in spite of the severity of the clinical picture. we conclude that vibrio vulnificus infection must be considered in ... | 1998 | 10079541 |
| activity of bacterial phosphomonoesterases in batch culture. | the phosphomonoesterases catalyse the hydrolysis of primary esters of phosphoric acid which help the bacteria to survive in phosphate stressed environment. ninety-five bacterial isolates were obtained from domestic sewage and industrial effluents of gelatine and soap factories at jabalpur on a medium enriched with phosphate and were screened for phosphatase production. the phosphatase producers were tentatively identified as escherichia coli, vibrio vulnificus, aeromonas hydrophila, staphylococc ... | 1998 | 9536656 |
| influence of water temperature and salinity on vibrio vulnificus in northern gulf and atlantic coast oysters (crassostrea virginica). | this study investigated the temperature and salinity parameters associated with waters and oysters linked to food-borne vibrio vulnificus infections. v. vulnificus was enumerated in oysters collected at three northern gulf coast sites and two atlantic coast sites from july 1994 through september 1995. two of these sites, black bay, la., and apalachicola bay, fla., are the source of the majority of the oysters implicated in v. vulnificus cases. oysters in all gulf coast sites exhibited a similar ... | 1998 | 9546182 |
| occurrence of vibrio vulnificus biotypes in danish marine environments. | during the unusually warm summer in denmark in 1994, 11 clinical cases of vibrio vulnificus infection were reported. these reports initiated an investigation of the occurrence of v. vulnificus biotypes in danish marine environments. samples of coastal water, sediment, shellfish, and wild fish were analyzed by preenrichment in alkaline peptone water amended with polymyxin b (2.0 x 10(4) u/liter) followed by streaking onto modified cellobiose-polymyxin b-colistin agar. v. vulnificus-like colonies ... | 1998 | 9435055 |
| phages infecting vibrio vulnificus are abundant and diverse in oysters (crassostrea virginica) collected from the gulf of mexico. | phages infecting vibrio vulnificus were abundant (> 10(4) phages g of oyster tissue-1) throughout the year in oysters (crassostrea virginica) collected from estuaries adjacent to the gulf of mexico (apalachicola bay, fla.; mobile bay, ala.; and black bay, la.). estimates of abundance ranged from 10(1) to 10(5) phages g of oyster tissue-1 and were dependent on the bacterial strain used to assay the sample. v. vulnificus was near or below detection limits (< 0.3 cell g-1) from january through marc ... | 1998 | 9435088 |
| evidence that expression of the vibrio vulnificus hemolysin gene is dependent on cyclic amp and cyclic amp receptor protein. | glucose repressed hemolysin production in vibrio vulnificus. promoter activity of the hemolysin gene, vvh, assessed with a vvh-luxcdabe transcriptional fusion, required cyclic amp (camp) and camp receptor protein (crp) in escherichia coli. hemolysin production in v. vulnificus increased after the addition of camp and was undetectable in a putative crp mutant, suggesting that vvh is also regulated by camp-crp in v. vulnificus. | 1999 | 10601227 |
| [a case of a sudden death from vibrio vulnificus septicemia in a patient with liver cirrhosis]. | a 46-year-old male patient with alcoholic cirrhosis of the liver was carried to our out-patient clinic as he had developed shock while under routine follow-up, and died on the way to the hospital. he had been admitted several times since the diagnosis eight years ago, and was finally discharged from the hospital six weeks ago with improved physical condition and laboratory findings. a vesicle and bulla formation with phlegmon on the skin of right leg and sole of foot was noticed. vibrio vulnific ... | 1999 | 10624097 |
| molecular typing of vibrio vulnificus isolated from clinical specimens by pulsed-field gel electrophoresis and random amplified polymorphic dna analysis. | primary v. vulnificus septicemia has been continuously reported in korea. we analyzed the molecular diversity of v. vulnificus strains isolated from clinical specimens using pulsed-field gel electrophoresis (pfge) and random amplified polymorphic dna (rapd) analysis. we analyzed a total of 23 v. vulnificus strains isolated from 22 patients between 1992 and 1997. rapd analysis was performed using five random primers, and we obtained chromosomal dna restriction patterns with noti and sfii by pfge. ... | 1999 | 10816612 |
| skin and soft-tissue infections after injury in the ocean: culture methods and antibiotic therapy for marine bacteria. | isolated organisms from two common indo-pacific marine animals (echinometra mathaei urchins and acanthaster planci sea stars) likely to cause puncture wounds to recreational beachcombers, diverse, or operational military forces during amphibious assaults demonstrate why practitioners should consider their first choice for potential antibiotic therapy differently from their usual favorite antibiotics. the effects of thiosulfate-citrate-bile-sucrose (tcbs) agar, varying salt concentrations in the ... | 1999 | 10091493 |
| the hemagglutinating action of vibrio vulnificus metalloprotease. | vibrio vulnificus protease (vvp), a 45-kda zinc metalloprotease, consists of two functional domains: an n-terminal 35-kda polypeptide having endoproteinase activity, and a c-terminal 10-kda polypeptide that mediates the binding of vvp to the erythrocyte membrane. therefore, vvp, but not its n-terminal endoproteinase domain alone, has agglutinating activity to rabbit erythrocytes. when a single zinc atom in the catalytic center was substituted by treatment with cucl2 or nicl2, proteolytic and hem ... | 1999 | 10100751 |
| ferric-reductase activities in vibrio vulnificus biotypes 1 and 2. | in this paper, the ferric-reductase activities of vibrio vulnificus were investigated. this species comprises two biotypes pathogenic for humans and eels that are able to express different mechanisms for iron acquisition. all strains of both biotypes used in this study were able to reduce ferric citrate, irrespective of the iron levels in the growth medium. some variation in the degree of reduction was observed among the strains, with the highest values corresponding to one acapsulated environme ... | 1999 | 10188249 |
| [vibrio vulnificus infection: clinical and bacteriological analysis of four cases]. | vibrio vulnificus (v. vulnificus) infection has recently been drawing attention as a high mortality disease especially in a patient with the preexisting chronic liver disease. the illness caused by v. vulnificus is divided into three groups such as primary septicemia, wound infection and gastrointestinal illness. primary septicemia, which is the most common in japan, is defined as a systemic illness presenting fever or hypotension with recovery of v. vulnificus from blood or tissue without the a ... | 1999 | 10213992 |
| differential expression of vibrio vulnificus capsular polysaccharide. | vibrio vulnificus is a human pathogen whose virulence has been associated with the expression of capsular polysaccharide (cps). multiple cps types have been described; however, virulence does not appear to correlate with a particular cps composition. reversible-phase variation for opaque and translucent colony morphologies is characterized by changes in cps expression, as suggested by electron microscopy of cells stained nonspecifically with ruthenium red. isolates with opaque colony morphologie ... | 1999 | 10225881 |
| indole-positive vibrio vulnificus isolated from disease outbreaks on a danish eel farm. | vibrio vulnificus was isolated in 1996 from 2 disease outbreaks on a danish eel farm which used brackish water. a characteristic clinical sign was extensive, deep muscle necrosis in the head region. v. vulnificus was isolated from kidney, mucus, spleen, gill and intestine of diseased eels. thirty-two isolates were examined phenotypically and serologically for pathogenicity to eels and for correlation to ribotype and plasmid profile. biochemically, the isolates showed properties similar to those ... | 1999 | 10228875 |
| pathogenesis of vibrio vulnificus. | this review describes the factors which are currently recognized as being central to the virulence of the human pathogen, vibrio vulnificus. this estuarine/marine bacterium occurs in high numbers in molluscan shellfish, primarily oysters, and its ingestion in raw oysters results in a ca. 60% mortality in those persons who are susceptible to this bacterium. the organism is also able to produce life-threatening wound infections. we describe here the nature of both the wound and primary septicemia ... | 1999 | 10339810 |
| occurrence, diversity, and pathogenicity of halophilic vibrio spp. and non-o1 vibrio cholerae from estuarine waters along the italian adriatic coast. | the occurrence, diversity, and pathogenicity of vibrio spp. were investigated in two estuaries along the italian adriatic coast. vibrio alginolyticus was the predominant species, followed by vibrio parahaemolyticus, non-o1 vibrio cholerae, and vibrio vulnificus. by using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. the production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number ... | 1999 | 10347072 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| the use of a modified dakin's solution (sodium hypochlorite) in the treatment of vibrio vulnificus infection. | we report the first clinical use of a modified dakin's solution (0.025% sodium hypochlorite [naocl]) to halt the progress of severe cutaneous vibrio vulnificus infection in a critically ill patient. the regimen used arose from an initial in vitro study designed to examine the sensitivity of vibrio species to topical antimicrobial agents. twenty-eight wound isolates were tested against the following eight topical preparations: silver sulfadiazine (silvadene), nitrofurazone, mupirocin ointment (ba ... | 1999 | 10347673 |
| use of mutant strain for evaluating processing strategies to inactivate vibrio vulnificus in oysters. | vibrio vulnificus is a ubiquitous marine bacterium frequently isolated from shellfish and associated with severe and often fatal disease in humans. various control strategies to reduce the disease risk associated with v. vulnificus contamination in shellfish have been proposed. however, evaluating the efficacy of these control strategies is complicated because of the difficulty in distinguishing v. vulnificus from the high levels of background environmental vibrio spp. the purpose of this resear ... | 1999 | 10382646 |
| factors influencing in vitro killing of bacteria by hemocytes of the eastern oyster (crassostrea virginica). | a tetrazolium dye reduction assay was used to study factors governing the killing of bacteria by oyster hemocytes. in vitro tests were performed on bacterial strains by using hemocytes from oysters collected from the same location in winter and summer. vibrio parahaemolyticus strains, altered in motility or colonial morphology (opaque and translucent), and listeria monocytogenes mutants lacking catalase, superoxide dismutase, hemolysin, and phospholipase activities were examined in winter and su ... | 1999 | 10388697 |
| identification and initial characterization of elastase activity associated with vibrio cholerae. | strains of vibrio cholerae o1 (ogawa, inaba) and non-o1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. the elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a ph range between 7 and 8. comparative studies indicate that more than 60% of v. cholerae strains analyzed quantitatively produce more elastase in broth (two- to fourfold higher) than ... | 1999 | 10398830 |
| isolation of vibrio vulnificus from sea water and sand along the dan region coast of the mediterranean. | the occurrence of vibrio vulnificus incoastal sea water and sand was investigated. samples (286 in toto) were taken during the period between november 1993 and july 1994. ten v. vulnificus isolates (6.9%) were recovered from sea water and two isolates were recovered from sand (1.4%). the total isolation rate for this period was 4.2%. in a longer period of investigation, from june 1996 until june 1998 (24 months), 1,248 samples were taken and 205 v. vulnificus isolates were recorded (32.8%) in se ... | 1999 | 10413865 |
| seafood-associated disease outbreaks in new york, 1980-1994. | seafood-associated disease outbreaks in new york were examined to describe their epidemiology and to identify areas for prevention and control efforts. | 1999 | 10429753 |
| compartment syndrome of the forearm as the initial symptom of systemic vibrio vulnificus infection. | sepsis, cellulitis, and necrotizing fasciitis rarely have been described as causes of compartment syndrome. we report a case in which forearm compartment syndrome presented as the initial symptom of systemic infection. vibrio vulnificus, the etiologic pathogen of the compartment syndrome, was isolated from wound and blood cultures. the patient was treated with systemic antibiotic treatment and multiple forearm fasciotomies. the infectious process progressed rapidly, however and due to underlying ... | 1999 | 10447162 |
| vibrio vulnificus septicaemia and necrotizing fasciitis after a prick from the dorsal fin of a tilapia. | 1999 | 10450443 | |
| aeromonas sobria infection with severe soft tissue damage and segmental necrotizing gastroenteritis in a patient with alcoholic liver cirrhosis. | a 49-year-old man, who had a 3-year history of liver dysfunction but had not been treated, was admitted to the hospital with a sudden onset of fever and generalized muscle pain. he subsequently developed generalized purpura with scattered hemorrhagic bullae of the skin and massive bloody stools. aeromonas sobria was proven by culture of both blood and bullous fluid. in spite of the extensive treatment with antibiotics and other medications in the intensive care unit (icu), the patient went into ... | 1999 | 10469397 |
| perkinsus marinus extracellular protease modulates survival of vibrio vulnificus in eastern oyster (crassostrea virginica) hemocytes. | the in vitro effects of the perkinsus marinus serine protease on the intracellular survival of vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. results showed that protease-treated hemocytes were initially slower to internalize v. vulnificus than untreated hemocytes. after 1 h, the elimination of v. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. our d ... | 1999 | 10473449 |
| modified dakin's solution for cutaneous vibrio infections. | vibrio species, specifically vibrio vulnificus, are known to be endemic to warm saltwater environments. as a human pathogen they are capable of causing severe, progressive, necrotizing infections. the lesions are bullous in nature and often require wide surgical debridement due to the aggressiveness of this organism. the literature supports prophylactic antibiotic therapy for those with preexisting hepatic dysfunction or immunocompromise. the authors routinely implement prophylactic antibiotic c ... | 1999 | 10517465 |
| in situ analysis of the bacterial communities associated to farmed eel by whole-cell hybridization. | bacterial communities in water samples and eel slime were investigated by fluorescence in situ hybridization of whole bacterial cells in an eel intensive culture system over 1 year. a newly developed probe, matching 27 vibrio spp., and a specific probe for vibrio vulnificus were used. phylogenetic probes complementary to selected regions of the 16s and 23s ribosomal rna revealed that proteobacteria of the alpha and beta subclass were predominant in water and eel slime. members of the gamma subcl ... | 1999 | 10530037 |
| primary vibrio vulnificus bacteremia in a liver transplant recipient after ingestion of raw oysters: caveat emptor. | vibrio vulnificus is responsible for severe infections in chronically ill patients. organ transplant recipients are also at risk for severe infections due to v vulnificus. we report here the first case of v. vulnificus primary bacteremia due to raw shellfish consumption in a liver transplant recipient. all transplant patients should be cautioned against consuming uncooked seafood and warned about the risk of severe vibrio infections from seemingly innocuous wounds acquired in a salt water enviro ... | 1999 | 10532553 |
| clinical, epidemiological, and microbiological features of vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in israel. israel vibrio study group. | vibrio vulnificus is a gram-negative bacterium that causes septicaemia and wound infection. cases occur sporadically, and no previous outbreaks due to a common source or a clonal strain have been reported. in the summer and autumn of 1996 and 1997, an outbreak of invasive v. vulnificus infection occurred in israel in people who had recently handled fresh, whole fish purchased from artificial fish-ponds. | 1999 | 10543668 |
| effects of a commercial heat-shock process on vibrio vulnificus in the american oyster, crassostrea virginica, harvested from the gulf coast. | oysters (crassostrea virginica) harvested from the gulf coast, containing 10(2) to 10(4) most probable number (mpn) per gram of vibrio vulnificus, were subjected to a commercial heat-shock process. after 1 to 4 min at internal oyster meat temperatures exceeding 50 degrees c, shellstock oysters were shucked, chilled, washed, and packed. v. vulnificus and total bacterial levels in gulf coast oysters were significantly reduced from 1 to 4 logs in the finished product. similar reductions were not ob ... | 1999 | 10571315 |
| [studies on the zinc metalloprotease produced by vibrio vulnificus]. | 1999 | 10643296 | |
| effects of salinity and temperature on long-term survival of the eel pathogen vibrio vulnificus biotype 2 (serovar e). | vibrio vulnificus biotype 2 (serovar e) is a primary eel pathogen. in this study, we performed long-term survival experiments to investigate whether the aquatic ecosystem can be a reservoir for this bacterium. we have used microcosms containing water of different salinities (ranging from 0.3 to 3.8%) maintained at three temperatures (12, 25, and 30 degrees c). temperature and salinity significantly affected long-term survival: (i) the optimal salinity for survival was 1.5%; (ii) lower salinities ... | 1999 | 10049871 |
| randomly amplified polymorphic dna analysis of clinical and environmental isolates of vibrio vulnificus and other vibrio species. | vibrio vulnificus is an estuarine bacterium that is capable of causing a rapidly fatal infection in humans. a randomly amplified polymorphic dna (rapd) pcr protocol was developed for use in detecting v. vulnificus, as well as other members of the genus vibrio. the resulting rapd profiles were analyzed by using rflpscan software. this rapd method clearly differentiated between members of the genus vibrio and between isolates of v. vulnificus. each v. vulnificus strain produced a unique band patte ... | 1999 | 10049874 |
| isolation of vibrio vulnificus serovar e from aquatic habitats in taiwan. | the existence of strains of vibrio vulnificus serovar e that are avirulent for eels is reported in this work. these isolates were recovered from water and oysters and differed from eel virulent strains in (i) fermentation and utilization of mannitol, (ii) ribotyping after hindiii digestion, and (iii) susceptibility to eel serum. lipopolysaccharide of these strains lacked the highest molecular weight immunoreactive bands, which are probably involved in serum resistance. | 1999 | 10049908 |
| the ability of vibrio vulnificus to use a synthetic hydrophilic heme compound, fe-tpps, as a single iron source. | vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. this process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin ix). in the present study, we tested whether this pathogen also uses a synthetic heme compound, fe-alpha,beta,gamma,delta-tetraphenylporphine tetrasulfonic acid (fe-tpps), as an iron source. when inoculated into a medium containing fe-tpps, v. vulnificus l-180 multiplication was seen ... | 1999 | 10079530 |
| adaptive response to cold temperatures in vibrio vulnificus. | the effectiveness of rapid chilling or freezing of oysters to reduce vibrio vulnificus levels in shellfish may be compromised by product handling procedures that permit cold adaptation. when a v. vulnificus culture was shifted from 35 degrees c to 6 degrees c conditions, it underwent transition to a non-culturable state. cells adapted to 15 degrees c prior to change to 6 degrees c condition, however, remain viable and culturable. in addition, cultures adapted to 15 degrees c were able to survive ... | 1999 | 9922468 |
| comparative study of biological properties and electrophoretic characteristics of lipopolysaccharide from eel-virulent and eel-a virulent vibrio vulnificus strains. | in vibrio vulnificus, virulence for eels is associated with serovar e strains. in this study, we investigated some biological properties of purified lipopolysaccharides (lpss) from serovar e and non-serovar e strains. purified lpss retained their o-polysaccharidic side chains and did not show any differences that could be related to host specificity, except for serological differences. | 1999 | 9925630 |
| activation of particulate guanylyl cyclase by vibrio vulnificus hemolysin. | recently we reported that vibrio vulnificus hemolysin, an exotoxin produced by v. vulnificus, dilates rat thoracic aorta via elevated cgmp levels without affecting nitric oxide synthase. we investigated the mechanism further by observing the guanylyl cyclase activities in cytosolic, membrane, unfractionated, or reconstituted preparations. hemolysin did not activate guanylyl cyclase in the membrane or cytosolic fraction, while it activated guanylyl cyclase in unfractionated or reconstituted prepa ... | 1999 | 9988111 |
| low incidence of vibrio vulnificus among vibrio isolates from sea water and shellfish of the western mediterranean coast. | a specific search for vibrio vulnificus in natural marine samples from the spanish mediterranean sea was carried out by nested pcr and cultural approaches using thiosulphate-citrate-bile salts-sucrose agar (tcbs) and cellobiose-polymixin b-colistin agar (cpc), incubated at 40 degrees c, as selective media. presumptive colonies were identified by pcr using specific primers against 23s rrna sequences. this species was isolated from sea water and edible bivalves, mainly after preenrichment in alkal ... | 1999 | 10030016 |
| survival of vibrio vulnificus in whole blood from patients with chronic liver diseases: association with phagocytosis by neutrophils and serum ferritin levels. | vibrio vulnificus causes severe wound infections and sepsis, mostly in persons with chronic liver diseases. survival of this organism in the whole blood collected from healthy volunteers and patients with chronic hepatitis, liver cirrhosis, and hepatoma was analyzed as an indication of susceptibility. the bacterial numbers in the blood after 5 h of incubation tended to increase with the severity of the liver disease and differed significantly between hepatoma patients and healthy volunteers (p<. ... | 1999 | 9841854 |
| [antibiotic resistance and plasmid profiles of vibrio isolates from cultured sparus sarba]. | a total of 51 potential pathogenic vibrios were isolated from moribund silver seabream sparus sarba, which were collected from local fish farms of hong kong. all the isolates were classified and identified as 7 species by the api 20 e system and the scheme of alsina & blanch. these species were vibrio alginolyticus (24 strains), vibrio vulnificus (12 strains), vibrio parahaemolyticus(7 strains), vibrio logei(4 strains), vibrio pelagius ii(2 strains), vibrio fluvialis (1 strains) and vibrio medit ... | 1999 | 12555529 |
| substrate specificity of hexosaminidase a isolated from the liver of a patient with a rare form (ab variant) of infantile gm2 gangliosidosis and control tissues. | 1999 | 96668 | |
| [retinal neovascularization: topological, morphological and hemodynamic features (author's transl)]. | 1999 | 91317 | |
| incidence of prostatic calcification in blacks in washington, d.c., and selected african cities. correlation of specimen roentgenographs and pathologic findings. cooperative prostatic research group. | the incidence of calcification in the prostate gland of black men from washington, d.c., and from ibadan, nigeria, and accra, ghana, west africa, was assessed in a total of 874 consecutive, unselected prostate specimens removed at autopsy during a five-year period (1973--1978). in the combined series there was a significant positive association between prostatic calcification and age (p less than 0.001). the frequency of calcification was significantly higher in the washington, d.c. series than ... | 1999 | 91253 |
| haloperidol and lithium blocking of the mood response to intravenous methylphenidate. | the euthymic manic-d-pressive patients with therapeutic plasma lithium levels were each given two i.f. infusions of 30 mg of methylphenidate. the infusions were separated by at least 3 days. before one infusion each patient was given 5 mg of haloperidol i.v. and before the other infusion each was given an identical volume of saline. a psychiatric observer was blind to the nature of the pretreatment and the order of pretreatment was randomized. saline pretreated patients showed marked activation ... | 1999 | 96473 |
| primary care approaches to developmental disabilities. | ninety-seven board certified pediatricians who spend at least 75% of their professional working hours involved in the delivery of primary care in new england were interviewed to explore their attitudes and current clinical approaches to developmental disabilities. the majority of the pediatricians relied exclusively on clinical judgment and general observations for assessing developmental problems in their offices. responsibility for preschool screening for potential learning problems and the as ... | 1999 | 91155 |
| unmasking of fetal determinants on adult bone marrow cells. | 1999 | 91109 | |
| sterilisation issues. | exception is taken to the 2 bases for sterilization of mentally handicapped people as stated by the working group in current medical/ethical problems -- genetic and fitness for parenthood. although parents with certain forms of retardation have a greater genetic propensity to bear mentally retarded offspring, there are considerably more "normal" parents with as high or a greater propensity. 80-90% of mentally handicapped children are born to normal parents. the unfitness for parenthood rationale ... | 1999 | 91007 |
| retinol-binding protein in malnutrition. | 1999 | 90984 | |
| analysis of the yolk proteins in drosophila melanogaster. translation in a cell free system and peptide analysis. | 1999 | 103753 | |
| [palliative chemotherapy with 5 fu and cpm in cancer of the prostate with bone metastases resistant to oestrogens. a clinical trial (author's transl)]. | the efficacy of an association of cyclophosphamide (cpm) and 5-fluorouracil was studied in 15 patients with prostate cancer not responding to oestrogen therapy, and more particularly its effect on pain due to bone metastases. no objective improvement was noted with this association, but there was a definitite reduction in bone metastases pain in 5 of the patients, with an average remission time of 4 months. half of the patients had nausea and vomiting, but in spite of this digestive intolerance ... | 1999 | 94108 |
| the mesencephalic exterolateral posterior nucleus of the mormyrid fish bryenomyrus niger: efferent connections studied by the hrp method. | 1999 | 91414 | |
| the treatment of systemic cryptococcosis with 5-fluorocytosine. | 1999 | 4531901 | |
| [statistical analysis of the ecg r-r intervals in certain types of heart rhythm disorders]. | 1999 | 91156 | |
| acute eosinophilic leukemia. an ultrastructural study. | observations on a 32 year old male are described. hematological examination demonstrated leukocytosis with circulating blastosis and dystrophic hypereosinophilia of the blood and bone marrow, with cells at various stages of maturation. cytotoxic chemotherapy led to complete remission for 5 months followed by a terminal relapse. no features in favor of an "eosinophilic collagenosis" were revealed at autopsy. repetitive ultrastructural studies performed during evolution of the illness demonstrated ... | 1999 | 96582 |
| the role of algal antigenic determinants in the recognition of potential algal symbionts by cells of chlorohydra. | algal cells grown in the green hydra chlorohydra viridissima were shown to possess characteristic antigenic determinants not found in algae cultured in vitro. these antigenic determinants, including those localized on the algal cell surface, were shown to be responsible for the phagocytic recognition of potential algal symbionts by digestive cells of chlorohydra. the results of this study indicate the existence of two systems governing phagocytosis in chlorohydra, one specific for algal cells gr ... | 1999 | 84815 |
| [renewal of noradrenaline in different zones of the central nervous system of inbred mice strains and their recombinants]. | a good correlation exists between the learning capacity and norepinephrine metabolism in the neocortex of c57 and balb inbred mice strains, as well as their f1 hybrids and seven recombinant inbred strains derived from their cross. the animals with a better learning performance are characterised by low levels of norepinephrine, as well as a slow metabolic rate of this neurotransmitter in the cortex. such a correlation has not been found to exist in the other cerebral regions studied. | 1999 | 96976 |
| group systems of serum proteins and erythrocyte enzymes in malignant lymphoma. | four group systems of serum proteins (hp, gc, gm, km) and five group systems of erythrocyte enzymes (ap, pgm1, gpt, ak, esd) were determined in 63 patients with malignant lymphoma. statistical analysis of the distribution of the above mentioned systems in patients and polish population samples did not reveal any significant differences, which points to the lack of any correlation between the disease and the group systems under examination. | 1999 | 85572 |
| [treatment of acute diabetic ketoacidosis by administering small insulin doses into the serum]. | 1999 | 99774 | |
| tetralogy of fallot in adults. | 1999 | 91440 | |
| chido, rodgers and c4. in vivo and in vitro coating of red blood cells, grouping and antibody detection. | c4 sucrose/low ionic strength (lis)-coated red blood cells (rbc) are excellent for the detection of the previously 'nebulous' antibodies, anti-chido and anti-rodgers, as well as for serum/plasma typing of these antigens by an inhibition technique. by enzyme treatment of such cells, it is confirmed that the ch and rg antigens reside on the c4d part of the c4 molecule. freshly taken rbc from normal individuals were examined with a sensitive auto-analyzer technique with anti-chido, anti-c4 and anti ... | 1999 | 91269 |
| [radiologic features of sudeck-kienböck's atrophy (author's transl)]. | 1999 | 91189 | |
| the relationship between intracellular and extracellular potassium in normal and malnourished subjects as studied in leukocytes. | leukocyte potassium has been measured in normal subjects and in malnourished children with and without edema both on admission and during recovery. in nonedematous malnourished children the potassium content was low (328 mmol/kg dry solids) and took 2-3 weeks of rehabilitation to return to normal (374 mmol/kg dry solids). leukocytes from edematous children had normal intracellular potassium values. however, 5 days on a maintenance diet reduced the intracellular potassium in the children with kwa ... | 1999 | 99717 |
| n-acetylprocainamide pharmacokinetics in functionally anephric patients before and after perturbation by hemodialysis. | napa pharmacokinetics were studied in 6 functionally anephric patients. distribution and nonrenal elimination of this drug were found to be the same as in individuals with normal renal function but renal clearance was reduced, resulting in a mean elimination t 1/2 of 41.9 hr (6.2 hr in normal subjects). renal clearance of napa correlated well with clcr. dialysis removed napa from both red blood cells and plasma and increased clt approximately fourfold. dialysis itself resulted in a 77% reduction ... | 1999 | 91468 |
| hexacarbon neuropathy. | 1999 | 91030 | |
| [pituitary reserve of thyrotrophic hormone in hypothyroid children]. | 1999 | 103634 |