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cell surface protein antigen from wolinella recta atcc 33238t.a high-molecular-weight (approximately 150,000) protein was selectively isolated by acid extraction from the cell surface of wolinella recta and purified by negative adsorption on deae-cellulose and gel filtration. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that this protein was found in w. recta but not in other wolinella species, such as w. curva and w. succinogenes. sera from patients with periodontitis reacted strongly with this protein antigen, whereas sera fr ...19892753998
enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens.enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ...19892754001
virulence of campylobacter jejuni for chicken embryos.the pathogenicity of campylobacter jejuni was examined in chicken embryos. in this system, mortality data and histopathological findings induced by organisms and by bacterium-free filtered broth were identical. the absence in chicken embryo tissues both of organisms and of an inflammatory infiltrate suggests a toxin etiology.19892754005
effects of sample holding time, temperature, and atmosphere on the isolation of campylobacter jejuni from dogs.stool specimens were collected from 39 dogs, inoculated onto campylobacter blood agar plates, and divided into four subsamples. subsamples were held at 4 and 25 degrees c in room air and in a microaerobic environment and were reinoculated at 1, 2, 3, 4, 6, and 8 h. c. jejuni survived at least 3 h when it was held at 4 degrees c, but less than 2 h when it was held at 25 degrees c. the holding atmosphere was not associated with a difference in isolation rates.19892754014
[endocarditis due to campylobacter jejuni and acquired immunodeficiency syndrome]. 19892756206
clinical and immunologic significance of cholera-like toxin and cytotoxin production by campylobacter species in patients with acute inflammatory diarrhea in the usa.the humoral immune response to both campylobacter jejuni cell surface antigens and to potential toxins of the organism was studied in 64 adults with inflammatory diarrhea. in an enzyme-linked immunosorbent assay (elisa) for surface antigens, 17 (71%) of 24 persons with campylobacter enteritis showed seroconversion in more than one immunoglobulin class, versus only 2 (5%) of 40 patients with non-campylobacter enteritis. in a gm1, ganglioside-based elisa for detecting serum igg to cholera-like ent ...19892760498
[biochemical and serologic characteristics of campylobacter jejuni/coli strains causing diarrhea in children].one hundred strains of campylobacter jejuni/coli isolated from faeces of children with diarrhoea were characterised. frequency of isolation of these microorganisms from faeces of children with enterocolitis symptoms was evaluated. in this group campylobacter jejuni/coli constituted 11.4% of all isolates, being the dominant etiologic agent of these infections. biotype pattern of 100 campylobacter jejuni/coli strains was determined. biotype i c.jejuni prevailed and c. coli constituted as much as 3 ...19892761320
the role of macrophages in experimental campylobacter-infections of mice in lagos, nigeria.the role of macrophages in experimental campylobacteriosis was studied in mice whose macrophages had been blocked using colloidal carbon particles as the blockading agent. two sets of mice received 0.4 ml intraperitoneally and 0.4 ml intravenously of colloidal carbon, respectively, and each set was then inoculated with a clinical isolate of campylobacter jejuni intraperitoneally. the treatment resulted in a lethality of 66.6% in mice given colloidal carbon intraperitoneally and 58.0% in mice giv ...19892763361
the gnotobiotic piglet as a model for the pathogenesis of campylobacter jejuni infection.the pathogenesis of enteric changes was studied in gnotobiotic piglets which, after hysterectomy had been infected orally with campylobacter jejuni on the first day of their life. the involvement of the entire large intestine became clinically manifest by scouring on days post infection (dpi) 4 to dpi 5, and pathomorphologically, by simultaneous inflammation and severe edema of the intestinal wall. histology and sem revealed inflammatory edema with abundant neutrophils, microulcerations, focal p ...19892765093
enhancement of campylobacter jejuni virulence by serial passage in chicks.four isolates of campylobacter jejuni were studied to determine changes in virulence following six serial passages in chicks. chicks that received invasive isolates exhibited diarrhea and depressed weight gain. immature mice were used to assess virulence of the passaged isolates of c. jejuni. nine-day-old mice infected with passaged isolates showed lethargy, dehydration, depression, decreased weight gain, and occult blood in feces. mouse pups inoculated with the third and sixth chick passage lev ...19892775092
waterborne outbreak of campylobacter enteritis after outdoors infantry drill in utti, finland.diarrhoea, abdominal pain, malaise and fever affected 75 of the 88 conscript soldiers in utti, finland after an outdoors infantry drill. campylobacter jejuni, heat-stable serotype 3/43/59, was isolated from 37 out of 63 men investigated. a clear serological response was evident in the risk group and negligible in the control group. the entire population at risk was interviewed. the outbreak was associated with the consumption of untreated surface water. c. jejuni, heat-stable serotype 3/43/59, w ...19892776848
isolation and characterization of catalase-negative and catalase-weak strains of campylobacter species, including "campylobacter upsaliensis," from humans with gastroenteritis.during 1987 and 1988, nine strains of catalase-negative or -weak campylobacter species were isolated in alberta, canada. dna hybridization studies demonstrated that seven strains were "campylobacter upsaliensis," one strain was highly homologous with campylobacter jejuni dna, and one strain was a campylobacter unrelated to the other two species. all "c. upsaliensis" strains were hippurate negative, and six of seven were susceptible to cephalothin. the unusual variant of c. jejuni was hippurate p ...19892778067
inhibition of enteropathogenic bacteria by human milk whey in vitro.diarrhea caused by enteropathogenic bacteria is a leading cause of childhood mortality world-wide, particularly in less developed regions. breast-feeding has been advocated to protect infants and children from infectious illnesses. we examined the antibacterial activity of human whey in vitro against multiple strains of the following species of enteropathogenic bacteria, campylobacter jejuni, escherichia coli, salmonella typhimurium, shigella flexneri, shigella sonnei and vibrio cholerae, all is ...19892787902
interaction of campylobacter species with antibody, complement and phagocytes.the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ...19892788111
[antibodies to campylobacter jejuni in patients with acute enteritis].in 233 patients with acute diarrhoea in paired sera campylobacter antibodies classes igg and iga were assessed by the elisa method. as antigen the external membrane protein of the strain campylobacter jejuni was used. raised igg levels (greater than 50 u.) and/or iga (greater than 80 u.) were found in 15% of all examined patients. a quadruple increase of values in one or both classes was recorded in 12.4% of the patients. the incidence of antibodies against campylobacter jejuni provides evidence ...19892790981
comparative in vitro activity of lomefloxacin, a difluoro-quinolone.lomefloxacin is a new difluoro-quinolone. in this study, we have determined the in vitro activity of lomefloxacin against a wide range of clinical bacterial isolates and compared it with that of other fluoro-quinolones and some unrelated antimicrobials. lomefloxacin was very active against enterobacteriaceae (mic90, 0.5 micrograms/ml) with activity comparable to that of ofloxacin (mic90, 0.25 micrograms/ml). lomefloxacin was moderately active against isolates of pseudomonas aeruginosa (mic90, 4 ...19892791500
[survey of campylobacter jejuni/coli infection and risk factors in children].from march to may 1987, 798 children under 7 years of the outpatients in chengdu district were examined and their stool samples cultured for campylobacter jejuni/coli. c. jejuni/coli was isolated from 31 (9.51%) of 326 children with diarrhea and 22 (4.66%) of 472 children with non-diarrhea. the difference was significant (p less than 0.05). this result indicated that c. jejuni/coli is an important pathogen of children diarrhea in chengdu district. meanwhile, by inquiry we investigated risk facto ...19892793148
microbial etiology of acute gastroenteritis in hospitalized children in kuwait.during a period of 15 months 621 hospitalized children with acute gastroenteritis and 152 control children were investigated for etiologic agents of the disease. putative enteropathogens were identified in 86% of the patients and 10% of the controls. common viral agents associated with gastroenteritis among children included rotaviruses (45%) and enteric adenoviruses (4%). bacterial pathogens infecting children were salmonella serotypes (24%), enterotoxigenic escherichia coli (9%), campylobacter ...19892797954
aeromonas-associated diarrhea in filipino children.aeromonas was isolated from the stools of ten out of sixty-six young (15%) suffering from acute diarrhea. these are the first reported cases in the philippines and the prevalence cited was higher than reported elsewhere. three species isolated were a. hydrophilia (50%), a. caviae (30%) and a. sobria (20%), either singly (60%) or in combination with etec and campylobacter jejuni. mean age of patients was 12.9 months with mean pre-admission duration of diarrhea of 48.6 hours. mean stool frequency ...19892798995
inhibition of campylobacter jejuni in vitro by broiler chicken caecal contents. 19892800258
in vitro activities of lomefloxacin and temafloxacin against pathogens causing diarrhea.the in vitro activities of temafloxacin (a63004) and lomefloxacin (sc-47111; ny-198) were compared with those of seven other antibiotics against 146 isolates of bacterial enteric pathogens, including campylobacter jejuni and campylobacter coli. ciprofloxacin was the most active drug against the salmonella, shigella, yersinia, and vibrio spp. tested. lomefloxacin, temafloxacin, and difloxacin were the most active drugs tested against campylobacter spp. (mic for 90% of strains, 0.125 to 0.25 micro ...19892802563
natural bactericidal activity of human serum against strains of campylobacter jejuni isolated in lagos, nigeria.the sensitivity of campylobacter jejuni strains la4, la12, la36, la53, la1214, la1308 and la1309 constituting the most prevalent strains of c. jejuni isolated in lagos, nigeria to the bactericidal activity of normal human serum was determined using microtiter technique. results obtained shows that only strains la 36 and la53 were serum resistant while others (strains la4, la12, la1214, la1308 and la1309) were serum sensitive. this finding is important epidemiologically and is the first report on ...19892805054
in-vitro activity of meropenem against clinical isolates obtained in canada.the in-vitro activity of meropenem, a new parenteral carbapenem, was compared with that of imipenem, ceftazidime, cefotaxime, piperacillin, gentamicin and, where appropriate, other antibiotics against recent clinical isolates and characterized beta-lactamase producers. mics were determined by a standard agar dilution procedure and two inocula (10(4) and 10(6) cfu) were used throughout. meropenem inhibited 90% of isolates of escherichia coli, klebsiella pneumoniae, proteus mirabilis, indole-posit ...19892808216
investigation of zoonotically acquired campylobacter jejuni enteritis with serotyping and restriction endonuclease dna analysis.five strains of campylobacter jejuni were isolated from asymptomatic coyotes being maintained in a vivarium. according to the results of serotyping with thermostable antigens and of bacterial chromosomal restriction endonuclease analysis, one of the c. jejuni strains isolated from the coyotes was responsible for enteritis in a laboratory animal technician providing husbandry for the laboratory-maintained coyotes.19892808666
etiology of acute childhood diarrhoea in calcutta.of the 152 cases of acute diarrhoea, 124 (81.5%) revealed potential pathogens. altogether 27 (21.2%) out of 127 strains of escherichia coli, klebsiella pneumoniae, enterobacter, proteus and acinetobacter produced enterotoxin. single pathogenic bacteria (40 cases 26.3%), parasite (6; 6%), rota virus (6; 6%), toxigenic bacteria (19; 12.5%) and mixed agents (37; 24.24.3%) were recorded in 108 cases (71.0%). another 14 (9.2%) cases exclusively revealed moderate to heavy growth of suspected enteric p ...19892815324
[dynamics of campylobacter jejuni invasion in gnotobiotic piglets].the study deals with the results of the investigation of c. jejuni populations in the intestines of gnotobiotic piglets after oral infection with c. jejuni in the span of one to five days after infection (dai). the infection of the whole large intestine was revealed, with the onset of pathological lesions 3rd dai. c. jejuni was isolated from liver, gallbladder, ileum, rectum, colon, mesenterial lymphatic node, rectal smears and blood. from 1st to 5th dai c. jejuni was demonstrated in liver, gall ...19892815491
early treatment of campylobacter jejuni enteritis.the bacteriologic and clinical effects of early antibiotic treatment of campylobacter jejuni enteritis were studied. erythromycin rapidly eliminated c. jejuni from stools, whereas trimethoprim-sulfamethoxazole did not. despite its bacteriologic effectiveness, erythromycin did not reduce the duration or severity of diarrhea, abdominal pain, or other symptoms.19892818711
saskatchewan. campylobacter jejuni abortion in beef cattle. 198917423403
seasonal prevalence of thermophilic campylobacter infections in dairy cattle and a study of infection of sheep.a total of 273 rectal swabs from dairy cows were cultured for campylobacter jejuni/coli. the isolation rate was 17/72 (24%), 33/106 (31%) and 11/95 (12%) during summer, autumn and winter respectively. approximately half of the isolates were c. jejuni and the other half c. coli. the isolates recovered from dairy cows were typed by bacterial restriction endonuclease dna analysis (brenda) and compared with those of sheep. seventeen different brenda patterns were produced by the isolates from dairy ...198916031500
saskatchewan. prevalence of campylobacter jejuni in pronghorns and mule deer in southern saskatchewan. 199017423564
prevalence of campylobacter jejuni in ranch mink at pelting: cultural, serological, and histological evidence of infection.this survey of 500 mink on three wisconsin ranches at pelting gives an estimate of the prevalence of campylobacter jejuni in the feces of clinically normal animals. on ranches 1 and 2, which used wet feed, c. jejuni was isolated by colon content culture from 7% and 32% of mink one year, and 43% and 13% the next year; the 200 bile samples tested were culture-negative. on ranch 3, which fed a pelleted ration, the organism was never isolated. among culture-positive mink tested, 22 of 55 had bacteri ...199017423585
campylobacter jejuni abortions in two beef cattle herds in saskatchewan.abortions, accompanied by placental retention and weight loss, occurred during february and march in 19% of 120 and 10% of 108 beef cows and heifers on two neighboring ranches in southern saskatchewan. a diagnosis of campylobacter jejuni abortion was made based on lesions of necrotizing and suppurative placentitis and fetal bronchopneumonia in association with the culture of large numbers of c. jejuni from placentas and fetal tissues.campylobacter jejuni was isolated with variable frequency from ...199017423586
[rotavirus diarrhea in children hospitalized in wrocław clinics].studies have been performed in 250 children aged 0 to 3 years suffering from diarrhoea. rotaviruses have been founded in 25% of cases, most frequently in children aged 0 to 3 months (29.6%), and 6 to 12 months (21.8%). frequency of rotavirus infections was highest during february. in 26% of subjects studied the coexisting infection with enteropathogenic escherichia coli strains or salmonella and campylobacter jejuni has been observed. the rotazyme ii test is more sensitive than the rotalex and r ...19901965233
guillain-barré syndrome and campylobacter jejuni infection. 19901971411
jackdaws as potential source of milk-borne campylobacter jejuni infection. 19901971891
bird attack on milk bottles: possible mode of transmission of campylobacter jejuni to man.a case-control study was carried out to test the hypothesis that the rise in the rate of campylobacter jejuni infection in the brigend area of south wales during may was due to the consumption or handling of milk from bottles that had been attacked by birds. 32 of 36 cases meeting the case definition were interviewed, along with 2 controls per case, matched for age, sex, and area of residence. there were strong associations between campylobacter infection and doorstep delivery of milk bottles, a ...19901978882
restriction fragment length polymorphism for the identification of campylobacter jejuni-isolates.companion animals ("pets") are occasionally carriers of organisms pathogenic for man. in the present, study fecal samples of clinically inapparent animals with direct contact to 204 patients, suffering from campylobacter enteritis, were investigated for c. jejuni or c. coli (cjc). cjc positive animals were seen in the environment of only five patients (= 2.4%). by comparison of biotypes and serotypes of thermostable and thermolabile antigens from human and animal isolates no clear epidemiologica ...19901982490
dna restriction digest and ribosomal rna gene patterns of campylobacter jejuni: a comparison with bio-, sero-, and bacteriophage-types of united kingdom outbreak strains.dna restriction endonuclease (hae iii and hind iii) total digest and 16s and 23s ribosomal (r)rna gene patterns (ribopatterns) were determined for 18 isolates of campylobacter jejuni from three separate outbreaks of diarrhoea in the north of england. strains were also characterized by biotyping, serotyping and phage typing. comparisons of the dna patterns by visual and numerical methods revealed five distinct strain groupings with clear differences between isolates from different outbreaks as we ...19901698647
detection of antibodies to helicobacter pylori cell surface antigens.serum igg antibodies of helicobacter pylori were detected in single-dilution elisa using glycine extracted material. among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90%) was found in patients with positive gastric culture for h. pylori than in culture negative patients (37%). among 68 blood donors the frequency of h. pylori antibodies was 28%. in 73 children less than 15 years of age examined for unrelated disorders the occurrence was 4%. by immunoblottin ...19901699265
[the specificity of campylobacter jejuni antigens in immunoenzyme analysis].the surface antigen has been obtained from c. jejuni culture and its immunochemical analysis has been carried out by the methods of immunoelectrophoresis, double diffusion with homologous and heterologous rabbit sera. the protein profile of this antigen has been studied by means of vertical electrophoresis in polyacrylamide gel with sodium dodecyl sulfate, and the molecular weight of the protein components of the antigen has been determined. the presence of anti-campylobacter antibodies has been ...19901711263
in vitro activity of tosufloxacin against bacterial enteric pathogens.the in vitro activity of tosufloxacin (a-61827), a new quinolone antibiotic, was compared with that of four other quinolones against 162 bacterial enteric pathogens. susceptibility testing was performed by using an agar dilution method. the minimal inhibitory concentration (mic) was defined as the lowest concentration of antibiotic without visible growth. tosufloxacin was the most active agent against campylobacter jejuni and c. coli, and ciprofloxacin was the most active agent against salmonell ...19902076595
six enteropathogens isolated from a case of acute gastroenteritis.isolation of six different gastrointestinal pathogens (entamoeba histolytica, plesiomonas shigelloides, campylobacter jejuni and three different salmonella species [salmonella typhimurium, salmonella blockley and salmonella hadar]) in the feces of a german female tourist suffering from acute diarrhoea after a trip to bali.19902076911
[infantile diarrheal diseases in madagascar: bacterial, parasitologic and viral study].from november 1988 to october 1989, an etiological study showed off the prevalence and the part of several enteropathogen agents which are not yet studied in madagascar. 1,523 stool's samples from 884 children with diarrhea and 639 children without diarrhea from 0 to 14 years old have been investigated. a bacterial, parasitical or viral etiology was found from 36.3% of diarrheic children and 11.2% of healthy children. the three agents the most frequently identified from children with diarrhea ar ...19902078082
identification of campylobacter jejuni and campylobacter coli from waste water by sds-disc-page of whole cell protein.analysis of the electrophoretic profiles of whole cell proteins of campylobacter strains from waste water was used to differentiate c. jejuni from c. coli. the results show, that biotyping of campylobacters from waste water may results in a false species differentiation. some of the campylobacter strains originally typed as c. coli, were proved to be atypical c. jejuni strains. therefore, in addition to biotyping and serotyping, electrophoretic typing is recommended to ensure correct identificat ...19902080967
[evaluation of a latex agglutination method for the rapid identification of campylobacter jejuni/coli].a latex agglutination assay was developed to identify campylobacter jejuni and campylobacter coli. we evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) to prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 mcfarland unit, and heated at 121 degrees c for 10 to 30 min. 2) bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without ...19902082036
[value of microbiologic studies for diagnosis of post-enteritis reactive arthritis].reactive arthritis may develop within a period of some days until upto 3 weeks after infections with yersinia enterocolitica, yersinia pseudotuberculosis, campylobacter jejuni/coli, shigella and salmonella. intestinal infections with klebsiella pneumoniae, clostridium perfringens, clostridium difficile, cryptosporidium, strongyloides stercoralis, taenia saginata and schistosoma mansoni are, in some cases, considered to be responsible for reactive arthritis. detection of pathogenic bacteria in fe ...19902085058
campylobacter jejuni peritonitis during chronic ambulatory peritoneal dialysis (capd).a patient treated with chronic ambulatory peritoneal dialysis developed recurrent peritonitis. during a fourth episode, campylobacter jejuni was cultured from the dialysate. she responded well to streptokinase and imipenem.19902085604
aetiology of bacterial diarrhoea in north indian children with special reference to campylobacter jejuni.a total of 184 cases of acute diarrhoea, in patients aged 1-36 months, were studied clinicobacteriologically. enteropathogens were isolated in 22.28% cases. campylobacter jejuni was isolated from 2.17% cases only. salmonellae were isolated from 7.6% cases and 64.28% of them were s typhimurium. more than 75% of all isolates of salmonellae, shigellae, esch coli and campylobacter jejuni were sensitive to gentamicin and cephaloridine. secondary lactose intolerance was noted in 6.52% cases only.19902090681
[a 3-year follow-up study of the incidence of campylobacteriosis in a pediatric population].the authors investigated the incidence of campylobacteriosis in the population of five paediatric health communities of the jihlava policlinic (5831 children) for a period of three years. a total of 2408 specimens faeces from 1501 subjects were examined. campylobacter jejuni was detected in 151 subjects (10.1%), salmonella sp. in 47 (3.1%), shigella sp. in 18 (1.2%), yersinia enterocolitica in 12 (0.8%) and enteropathogenic escherichia coli in five subjects (0.3%). the total incidence of campylo ...19902092913
laboratory findings associated with abomasal ulcers/tympany in range calves.the etiology of abomasal ulcers/tympany was investigated in 48 animals from 36 ranches in wyoming and nebraska. results indicate that subclinical trace mineral deficiencies of copper and/or selenium exist in the range cattle in west central nebraska and wyoming. etiological agents most frequently incriminated by bacteriologic cultures and/or histopathic examination were clostridium perfringens and campylobacter species. histopathologic evaluation of abomasums revealed 31 of 38 cases contained ab ...19902094446
[comparative study of campylobacter pyloridis and campylobacter jejuni under electron microscope].specimens were made by preparing the ultrathin sections after negative staining and ruthenium red staining. the specimens were observed under electron microscope. results revealed that the shapes of campylobacter pyloridis (cp) and c. jejuni (cj) were similar, but distinctive differences in their structures were observed. cp had one to five sheathed flagella at one end with bulbous tips. cj had only one unsheathed flagellum on each end without a bulbous tip. the cell body of cp was longer than t ...19902094635
[primary study on pathogenesis of campylobacter jejuni enteritis].the invasiveness and heat-labile enterotoxin of 11 human strains of c. jejuni were detected in nih mice inoculated with the c. jejuni strains; the methods of plate immunological hemolysin test and spa-coagglutination test were employed. ten strains of the bacteria invaded the intestine; 7 strains (45.46%) were positive in spa-coagglutination test; and 4 (36.36%) produced enterotoxin. c. jejuni in chengdu area possess multiple pathogenicity, e.g invasiveness and heat-labile enterotoxin, but we di ...19902094644
immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis.antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ...19902099622
the incidence and epidemiological significance of campylobacter jejuni/coli serotypes in romania.the distribution per serotypes of 401 campylobacter strains was studied by lior's procedure, 241 strains being identified as c. jejuni (60.1%) and 160 as c. coli (39.9%): out of these 329 were of human origin (82%), including 225 c. jejuni and 104 c. coli, and 72 were isolated from fowls and swine (16 c. jejuni, 56 c. coli). out of the total of 401 strains, 363 (90.5%) were typable. among human strains, 32 serotypes were identified, more frequently encountered being (in a decreasing order) 29, 4 ...19902101205
management of acute diarrhoea.before 1970, laboratory staff could not only identify the causative organism of acute diarrhea in 20% of cases, but in 1990, they could identify it in 80% of cases. these organisms are either bacteria, virus, or parasites. the bacteria include enterotoxigenic bacteria (vibrio cholerae, enterotoxigenic escherichia coli, clostridium perfringens, and staphylococcus aureus) and enteroinvasive bacteria (campylobacter jejuni, c. coli, and salmonella and shigella species). the leading cause of death ...19902101385
travelers' diarrhea among u.s. navy and marine corps personnel during a western pacific deployment.the incidence and etiology of travelers' diarrhea was studied in a crew of 1,914 sailors and marines aboard a u.s. navy ship during a western pacific deployment. questionnaires completed by 301 troops indicated that 52% had at least one episode of diarrhea during the deployment; however, only 5% of the ship's company sought treatment. enterotoxigenic escherichia coli was the most commonly identified pathogen (23%), followed by giardia lamblia (6%), salmonella (3%), rotavirus (2%), and shigella, ...19902107464
microbiological quality and safety of zoo food.two types of commercial products for feeding zoo animals (a frozen meat product, referred to as zoo food, and a dry product, referred to as dry food) were microbiologically examined for spoilage organisms (aerobic, psychrotrophic, coliform, escherichia coli, mold, and yeasts) and pathogens (salmonella spp., listeria monocytogenes, and campylobacter jejuni). levels of microorganisms in frozen ground zoo food were compared with those in frozen ground beef and frozen ground turkey meat. the level o ...19902111115
acidity and intestinal bacteria: an in-vitro assessment of the bactericidal activity of hydrochloric acid on intestinal pathogens.the bactericidal activity of hydrochloric acid (hcl) on different intestinal bacteria was evaluated at various ph values and time intervals. the effect of hcl was highest at ph 2 and ph 3 on strains of campylobacter jejuni, aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica, salmonella spp. and shigella spp., producing complete killing after 60 min incubation. however, at higher ph values (ph 4 and above), these organisms not only survived but they also showed evidence of ra ...19902127996
[etiological research on acute gastroenteritis in the city of cádiz].we researched the microbial etiology of 5,836 diarrheal processes in outpatients and inpatients at the social security hospital in cádiz, for the purpose of evaluating their microbiological and epidemiological characteristics. bacterial gastroenteritis accounted for 51.6% of the cases, while 31.7% were viral and 16.6% were protozoal in origin. salmonella enterica (35.6%) is the most frequently involved microorganism, especially in the summer, followed very closely by rotavirus (31.7%). the incid ...19902129735
[occurrence of intestinal parasites and campylobacter jejuni in school-age children in krusevac and aleksandrovac]. 19902130015
chicken carcasses as a source of campylobacter jejuni in belo horizonte, brazil.the presence of campylobacter jejuni was investigated in stool specimens from chicken meat workers and in ready-for-market chicken carcasses from one industrial and nine non industrial slaughters in belo horizonte. in the latter c. jejuni was isolated from 19 (38.0%) of the 50 chicken carcasses and from 2 (13.3%) of the stool specimens obtained from 15 chicken meat workers. in the industrial slaughter it was found in only 1 (2.0%) of the 50 chicken carcasses and it was not isolated from any of t ...19902135487
[epidemiology of campylobacter jejuni/coli].the authors describe the epidemic incidence of diseases caused by campylobacter jejuni/coli. during the 5-week follow up period (june 1-july 1, 1988) a total of 74 subjects fell sick. in 31 instances the suspect factor of transmission was non-pasteurized cheese prepared from sheep's milk. in this group of patients a significant shift to higher age categories was noted, contrary to the other 43 diseases. it did not prove possible to isolate campylobacter from cheese, smears from the cottage or fr ...19902139812
campylobacter jejuni-induced vasculitis. 19902151678
[sensitivity of enteropathogenic bacteria to furazolidone].the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ...19902152485
clinical and parasitologic aspects of cryptosporidiosis in nonhuman primates.eighty-one cases of acute cryptosporidiosis were diagnosed among 157 (52%) infant primates, predominantly macaca nemestrina, housed in the nursery unit of the washington regional primate research center. the mean age at onset of oocyst passage was 38 +/- 25 days. the outbreak was confined to the nursery and no cases were detected among juvenile or adult primates housed in other rooms within the colony. all but one animal manifested symptoms of enteric infection, including severe diarrhea and deh ...19902153858
effect of enteroviruses on adherence to and invasion of hep-2 cells by campylobacter isolates.coinfection of hep-2 epithelial cells with coxsackievirus b3, echovirus 7, poliovirus (lsc type 1), porcine enterovirus, and campylobacter isolates was performed to determine if a synergistic effect could be obtained. the invasiveness of campylobacter jejuni atcc 33560 was significantly increased for hep-2 cells preinfected with echovirus 7, coxsackievirus b3, and uv-inactivated (noninfectious) coxsackievirus b3 particles. additionally, the invasiveness of c. jejuni m96, a clinical isolate, was ...19902156779
risk of diarrhea during the first year of life associated with initial and subsequent colonization by specific enteropathogens.the incidence of colonization by enteropathogenic, enterotoxigenic, enteroinvasive, and enterohemorrhagic escherichia coli (detected by dna hybridization with specific radiolabeled probes), salmonella sp., shigella sp., campylobacter jejuni, and rotavirus was related to the presence of diarrhea in a cohort of 75 rural infants followed longitudinally during the first year of life. the study was carried out between august 1985 and february 1987 in the village of lugar sobre la tierra blanca, in th ...19902157338
identification of campylobacter jejuni surface proteins that bind to eucaryotic cells in vitro.to understand the role of campylobacter jejuni surface proteins in the interaction of c. jejuni with cultured mammalian cell lines in vitro, we developed a ligand-binding assay. this procedure allowed us to antigenically identify c. jejuni outer membrane proteins (omps) that attach to intact host cell membranes. omps isolated from an invasive strain and a less invasive strain were antigenically indistinguishable. however, we found that proteins with molecular masses of 28 and 32 kilodaltons (kda ...19902160431
persistent excretion of rotavirus by pregnant cows.in a study of the epizootiology and prevalence of enteropathogens which may be involved in neonatal calf diarrhoea, 10 in-calf cows from a herd with a history of rotavirus-induced calf diarrhoea were monitored over a period of six to seven months. all the cows excreted rotavirus intermittently without showing any clinical signs, and 21.8 per cent of faecal samples contained rotavirus. reoviruses were isolated from 87 per cent of the samples from the cows, and from all the 10 calves born to them. ...19902164274
emergence of aminoglycoside resistance genes aada and aade in the genus campylobacter.resistance to streptomycin or spectinomycin or both in five campylobacter coli strains, two campylobacter jejuni strains, and a campylobacter-like strain was studied by enzymatic assays and dot blot hybridization. resistance was due to 6- or 3",9-aminoglycoside adenylyltransferases and to new types of phospho- and adenylyltransferases.19902168151
use of pulsed-field agarose gel electrophoresis to size genomes of campylobacter species and to construct a sali map of campylobacter jejuni ua580.to determine the physical length of the chromosome of campylobacter jejuni, the genome was subjected to digestion by a series of restriction endonucleases to produce a small number of large restriction fragments. these fragments were then separated by pulsed-field gel electrophoresis with the contour-clamped homogeneous electric field system. the dna of c. jejuni, with its low g+c content, was found to have no restriction sites for enzymes noti and sfii, which cut a high-g+c regions. most of the ...19902168376
catalase activity in campylobacter jejuni: comparison of a wild-type strain with an aerotolerant variant.a comparison of campylobacter jejuni vpi strain h840 (atcc 29428), which can grow at o2 levels up to 15%, with variant strain mc711-01 (which can grow at o2 levels up to 21-26%) indicated that the specific activity of catalase in crude cell extracts was higher in the variant by a factor of 1.6 to 2.5, depending on cultural conditions. smaller differences occurred with superoxide dismutase activity, while peroxidase activities were invariably lower in the variant strain. the variant strain was mu ...19902168804
biotypes, serogroups and antibiotic susceptibility of campylobacter jejuni and campylobacter coli in chile.phenotypic markers were studied in 105 strains of thermophilic campylobacters isolated from human beings, animals and drinking water in santiago, chile. the strains were identified as campylobacter jejuni (n = 49) and campylobacter coli (n = 56). biotypes i and ii (lior schema) accounted for 96% c. jejuni isolates, the other 4% being biotype iv but the two biotypes of c. coli were about equally represented. a total of 28 serogroups (lior's heat-labile antigens) were identified. lior 13, 9, 79, 2 ...19902181024
molecular studies on the mechanism of tetracycline resistance mediated by tet(o).the mechanism of resistance to tetracycline in escherichia coli mediated by the campylobacter jejuni-derived resistance determinant tet(o) was investigated. the cloned tet(o) protein had no detectable effect on the intracellular accumulation of tetracycline. the presence of tet(o) markedly diminished the inhibitory effect of tetracycline on protein synthesis both in vivo and in vitro. ribosomes prepared from tetracycline-resistant and susceptible e. coli cells bound almost identical amounts of r ...19902183711
factors responsible for the introduction and spread of campylobacter jejuni infection in commercial poultry production.campylobacter jejuni and related thermophilic campylobacters were not found in a hatchery or in chicks aged less than seven days. however, an increasing proportion of chicks aged two weeks and older shed campylobacters in their droppings. it was shown that a likely source of c jejuni for young chicks was the environment in the immediate vicinity of the rearing houses, and that infection could readily be introduced on the footwear and clothing of farm staff. thermophilic campylobacters were found ...19902188414
to the problem of campylobacter jejuni detectability in water.in a series of model experiments two isolation procedures for the detection of water-borne campylobacter jejuni were compared: a standard culture in thioglycolate broth enriched with 7% defibrinated sheep blood and supplement c and a modified membrane filtration method in which the filter (porosity 0.45 microns) plated on campylobacter agar surface was removed after the first 24 hours of incubation and the plate further incubated for 48 hours. the recovery rates by the thioglycolate broth method ...19902191035
[surgical treatment of infectious aneurysm of the popliteal artery. description of a case caused by campylobacter jejuni and a review of the literature].the authors describe a case of mycotic aneurysm of the popliteal artery secondary to campylobacter jejuni-derived infectious endocarditis treated by excision and in situ femoro tibial venous bypass, and take the opportunity to review 14 literature cases of infectious aneurysm of popliteal artery. this diagnosis is most frequently evoked by the development in an infectious setting of a throbbing inflammatory mass in the popliteal fossa. management is aimed at controlling the infection and insurin ...19902193936
[evaluation of effectiveness of rokitamycin dry syrup in acute enteritis in pediatrics. a comparative study on rokitamycin and fosfomycin dry syrups].because campylobacter jejuni is most frequently identified as a causative organism of bacterial enteritis in pediatrics, a study was done to evaluate the clinical efficacy against campylobacter enteritis and the safety of a macrolide antibiotic, rokitamycin (rkm). in case of acute enteritis, rkm was used in a form of dry syrup at a dose level of approximately 30 mg (in potency)/kg body weight and its efficacy and safety were compared to those of fosfomycin (fom) dry syrup which is currently in u ...19902194055
morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, alopex lagopus.in a blue fox farm about 300 of 400 weanling pups were affected with diarrhoea and prolapse of the rectum. about 40 pups died in an emaciated state after a few days of disease. eight pups were submitted for examination. pathological findings were restricted to the caecum, proximal colon and rectum, with occasional extension to the ileum. affected areas revealed a thick and rigid intestinal wall, a narrow lumen and a thickened, wrinkled and sometimes ulcerated mucosa. histologically, the mucosa s ...19902195067
vacation diarrhea. how should it be managed?traveler's diarrhea in north america is most often a self-limited disease that requires little medical intervention unless the course is prolonged or especially severe. diagnosis is almost always made by a stool examination or culture. the most common causative organisms are campylobacter jejuni and giardia lamblia. enterotoxigenic escherichia coli is a less frequent cause. patients should be advised regarding the need for fluid replacement. antibiotic therapy should be considered only in rare c ...19902195482
acute gastroenteritis due to double infection with enteropathogenic escherichia coli or salmonella and another bacterial pathogen.two enteric bacterial pathogens were concomitantly isolated from the feces of 18 infants less than 6 months of age admitted to the assaf harofeh medical center over a 7-year period. in all but two patients stool cultures grew enteropathogenic escherichia coli, six of serogroup 0119. the other organisms cultured were shigella, salmonella, campylobacter jejuni and aeromonas hydrophila. the usual clinical presentation was diarrhea, dehydration and vomiting of acute onset, and low grade fever. patie ...19902199408
isolation, characterization, and host-cell-binding properties of a cytotoxin from campylobacter jejuni.a 68,000-molecular-weight protein was isolated by polyacrylamide gel electrophoresis from the organism-free filtrate of a fully virulent clinical strain of campylobacter jejuni. the eluted protein was heat labile, was inactivated at either ph 3.0 or 9.0, was sensitive to trypsin, and was lethal for fertile chicken eggs. it also had toxic effects on chicken embryo fibroblast, chinese hamster ovary (cho), and intestinal 407 (int407) cells. a monoclonal antibody (cetpmab4) raised to this eluted tox ...19902199497
an outbreak of campylobacter enteritis associated with a community water supply.in june 1987, an outbreak of acute enteritis occurred over a period of 2 weeks in a small rural community where the potable water supply is unfiltrated and unchlorinated. campylobacter jejuni was isolated from 6 patients. a case-control study performed in a local fiberglass shop where workers drink a lot of water, showed an association between the occurrence of enteritis and the consumption of 10 or more 8-ounce glasses of water per day (odds ratio = 6, p = 0.04). the attack rate of enteritis wa ...19902207948
identification of catalase negative/weak campylobacter jejuni from human blood and faecal cultures by numerical analysis of electrophoretic protein patterns.twenty-eight isolates of catalase-negative/weak (cnw) thermophilic campylobacters from human blood and faecal cultures were characterized by one-dimensional (1-d) high-resolution sds-page of cellular proteins. a further 11 campylobacter strains were included for reference purposes. partial protein patterns were used as the basis for numerical analysis, which showed that all of the hippurate-positive strains had a high similarity to c. jejuni. two subclusters were formed within c. jejuni correspo ...19902210345
structural and functional analysis of two campylobacter jejuni flagellin genes.the purpose of this study was to characterize the flagellin gene of campylobacter jejuni and to study the structure of this protein and the regulation of its synthesis. a part of the flagellin gene of c. jejuni strain 81116 was recently cloned by us. this dna fragment was used as a probe to isolate the other homologous flagellin sequences from genomic libraries. the flagellin nucleotide sequence was determined from overlapping clones. two copies of the flagellin gene were identified: genes fla a ...19902211662
salmonella-reactive synovial fluid t-cell clones in a patient with post-infectious salmonella arthritis.from a patient with reactive arthritis following salmonella typhimurium enteritis, synovial fluid t-lymphocytes were cloned and expanded in vitro. seven out of 74 clones showed a marked proliferative response to antigens of heat-killed salmonella typhimurium with autologous t-cell-depleted peripheral blood mononuclear cells as antigen-presenting cells. the salmonella-reactive clones were of the cd4+ phenotype, antigen-induced proliferation could be inhibited by a monoclonal antibody to hla class ...19902218431
[etiology of diarrheal diseases in immunocompetent and hiv-positive patients].in 1986 and 1987 the stool samples of 206 patients of the medical outpatient clinic basel were examined prospectively for bacteria, protozoa and worms. clinical data of the patients were recorded by questionnaire. the patient group comprised 63 immunocompetent patients as well as 23 hiv-infected patients, all with symptoms of acute enteritis. the control group consisted of 120 healthy persons. pathogenic organisms were found in the stools of 17.5% of the immunocompetent patients with enteritis. ...19902218447
evaluation of cytotoxic activity in fecal filtrates from patients with campylobacter jejuni or campylobacter coli enteritis.we sought to determine the prevalence of cytotoxic activity in fecal filtrates from persons with c. jejuni or c. coli enteritis. stool specimens were collected from 20 persons with c. jejuni or c. coli enteritis, 20 persons with acute diarrheal illnesses of other causes, and 9 healthy, asymptomatic persons. fecal filtrates were then incubated with chinese hamster ovary (cho) or hela cells. the fecal filtrate from 1 of the 20 (5%) persons with campylobacter enteritis was cytotoxic for hela cells ...19902227365
[the serotype distribution of campylobacter jejuni strains among gastroenteritis in hospitals over 7 year period in tokyo].campylobacter jejuni strains isolated from gastroenteritis at 4 general hospitals of tokyo metropolitan during the period from 1981 to 1987 were serotype according to the slide agglutination test (tck system) developed by the tokyo metropolitan research laboratory of public health. two thousand four hundred seventy-nine strains isolated from sporadic cases among infants and children, 1,962 (78.5%) were typed by 33 typing sera numbered tck 1 through tck 33 and leaving 537 strains (21.5%) untypabl ...19902230367
[serology and culture diagnosis of campylobacter jejuni infections].campylobacter jejuni is known today as one of the most common pathogens in acute infectious enteritis. in 77 patients serological testing by complement fixation as well as stool cultures were performed. a campylobacter jejuni infection was identified in 53% by culture and in 64% by serology, only in 17% the diagnosis was made with both methods. we conclude, that because of these data stool culture and serological testing should be used simultaneously. the complement fixation technique shows an i ...19902233597
[identification of campylobacter jejuni by using a biotinylated probe].a dna biotinylated probe for the rapid identification of campylobacter jejuni in culture (enzo biochem, new york) has been evaluated. the hybridized biotinylated dna probe is detected by its interaction with streptavidin linked to horseradish peroxidase. sixteen strains of c. jejuni, including type strain. 24 strains of other campylobacter and helicobacter species, and 59 strains of other general have been studied. the probe was highly sensitive (100%) and specific (100% inside the genera campyl ...19902235085
some early observations on hiv infection in children at king edward viii hospital, durban.nine black children aged between 3 months and 30 months of age, with human immunodeficiency virus type i (hiv-i) infection are described to draw the attention of health professionals in southern africa to special clinical characteristics useful for recognising this problem, which has many shared features with common diseases of infancy and childhood in the third world. the main presenting complaints were chronic cough and persistent diarrhoea and vomiting. these children frequently had diarrhoea ...19902237685
influence of campylobacter jejuni cecal colonization on immunoglobulin response in chickens.the immunoglobulin response of chickens to colonization by campylobacter jejuni isolates b-540 and clin-1 was monitored. chicken humoral igg and biliary secretory iga (siga) responses were assessed by enzyme-linked immunosorbent assay (elisa). samples were taken from 128 c. jejuni-colonized chickens and 104 uncolonized chickens housed in a controlled environment. an indirect elisa was performed using the homologous isolate of c. jejuni as the capture antigen and was developed with the specific g ...19902241685
influence of antibody treatment of campylobacter jejuni on the dose required to colonize chicks.this study was designed to clarify the role of antibodies in controlling chicken colonization by campylobacter jejuni. cecal colonization by c. jejuni was compared after the organism was exposed either to phosphate-buffered saline, normal rabbit serum, rabbit hyperimmune anti-c. jejuni serum, or anti-c. jejuni antibodies extracted from chicken bile. antibodies from chicken bile were extracted by affinity absorption against outer-membrane proteins from the challenge organism. sera were heated 1 h ...19902241686
influence of host lineage on cecal colonization by campylobacter jejuni in chickens.the resistance to cecal colonization by campylobacter jejuni was assessed by challenging three crossbred stocks of commercially available broiler chickens. these three stocks, designated a, b, and c, were related as follows: offspring from four pedigreed grandparent flocks were used as progenitors. stock b was derived by cross-breeding grandparent 1 with grandparent 3. stocks a and c were crossbreeds from grandparents 1 and 2 and grandparents 3 and 4, respectively. campylobacter jejuni were gava ...19902241687
[the pathogenicity of campylobacter jejuni as a monoinfection and as a mixed infection with escherichia coli 078:k80 in broilers].two week old broilers (n = 61) with a monoinfection with campylobacter jejuni (0.5 ml of suspension containing 10(5) cfu/ml per os) showed reduced increase in weight during week 3 after infection compared to the control group. an other group of chickens (n = 31) was additionally infected with a suspension of escherichia (e.) coli o78:k80 via drinking water from day 4 to 6 after the primary infection. this mixed infection provoked clinical signs of a disease and reduced increase in weight during ...19902241888
size and physical map of the campylobacter jejuni chromosome.the chromosome of campylobacter jejuni is circular and approximately 1700 kb in circumference. the size of the genome was determined by field inversion gel electrophoresis of restriction endonuclease fragments using lambda dna concatamers and yeast chromosomes to calibrate the size of the fragments. in view of the low (32-35%) g + c content of the campylobacter genome, enzymes that recognizes gc-rich sequences were used. of the enzymes tested bsshii (g/c(g)cgc), ncii (cc/cgcg) and sali (g/tcgac) ...19902243769
study of haemolytic activity of some campylobacter spp. on blood agar plates.a total of 152 strains of campylobacter jejuni, c. coli, c. laridis and c. fetus subsp. fetus were tested for haemolysis on blood agar plates. distinct haemolysis was detected in 92.3% (96/104) of strains of c. jejuni and 21.7% (5/23) of strains of c. coli on sheep blood heart infusion agar after incubation for 4 d microaerobically at 42 degrees c. haemolysis was also detected on horse blood heart infusion agar. haemolysis was not detected at 37 degrees c except with one of 50 strains of c. jeju ...19902246143
acute axonal polyneuropathy associated with anti-gm1 antibodies following campylobacter enteritis.we report 2 patients with guillain-barré syndrome (gbs) following campylobacter jejuni enteritis. electrophysiologic studies indicated that the predominant process was axonal degeneration of motor nerves, and clinical recovery was poor. serum testing by thin-layer chromatography and enzyme-linked immunosorbent assay revealed that the sera from both patients contained high titers of igg antibody against gm1 ganglioside. these cases may represent a subgroup of gbs as acute axonal polyneuropathy fo ...19902247243
reproductive failure in mink and ferrets after intravenous or oral inoculation of campylobacter jejuni.four pregnant mink and seven pregnant ferrets, including five with previous exposure and specific antibody, were injected intravenously with 10(8)-10(10) colony-forming units of campylobacter jejuni. all 11 pregnancies failed 1-16 days after infection, with results ranging from fetal resorption to expulsion of dead or premature living kits. in every case, uterine contents (placenta, uterine fluid and/or kits) were culture-positive for c. jejuni. three pregnant mink and nine pregnant ferrets, inc ...19902249178
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