Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| multiplex pcr and multiplex rt-pcr for inclusive detection of major swine dna and rna viruses in pigs with multiple infections. | multiplex pcr and multiplex rt-pcr were developed to identify nine viruses in pigs with multiple infections. these viruses are: porcine circovirus type 2 (pcv2), suid herpesvirus 1, porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus (prrsv), japanese encephalitis virus, porcine rotavirus a (porv-a), porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and getah virus. these methods were shown to be high specificity and sensitivity. in the ... | 2009 | 19467264 |
| qtl for traits related to humoral immune response estimated from data of a porcine f2 resource population. | this study aimed to map quantitative trait loci (qtl) for traits related to humoral innate immune defence. therefore, haemolytic complement activity in the alternative and the classical pathway, serum concentration of c3c and of haptoglobin (hp) were measured in blood samples obtained from f2 piglets (n = 457) of a porcine f2 resource population before and after mycoplasma hyopneumoniae, aujeszky's disease virus (suid herpesvirus i, suhvi) and porcine reproductive and respiratory syndrome virus ... | 2009 | 19490209 |
| virion-incorporated glycoprotein b mediates transneuronal spread of pseudorabies virus. | transneuronal spread of pseudorabies virus (prv) is a multistep process that requires several virally encoded proteins. previous studies have shown that prv glycoprotein b (gb), a component of the viral fusion machinery, is required for the transmission of infection to postsynaptic, second-order neurons. we sought to identify the gb-mediated step in viral transmission. we determined that gb is not required for the sorting of virions into axons of infected neurons, anterograde transport, or the r ... | 2009 | 19494011 |
| propagation of swine hemagglutinating encephalomyelitis virus and pseudorabies virus in dorsal root ganglia cells. | swine hemagglutinating encephalomyelitis virus (hev) causes encephalomyelitis or vomiting and wasting disease in suckling piglets. neurotoropism of the virus has been demonstrated in previous in vivo studies. in the present study, we investigated the infectivity and propagation of hev in comparison with those of pseudorabies virus (prv), another neurotropic virus, using dorsal root ganglia cells of newborn mice containing nerve cells and non-neuronal cells. hev infected nerve cells but did not i ... | 2009 | 19498285 |
| pathogen exposure in feral swine populations geographically associated with high densities of transitional swine premises and commercial swine production. | surveys for evidence of exposure to pseudorabies virus (prv), brucella suis, swine influenza virus (siv; human-like h1n1, reassortant type h1n1, h1n2-like h1n1 and h3n2), porcine circovirus 2 (pcv 2), and porcine respiratory and reproductive syndrome virus (prrsv) in feral swine (sus scrofa) were conducted in areas where feral swine were geographically associated with high densities of transitional swine premises in south carolina and high densities of commercial swine production in north caroli ... | 2009 | 19617481 |
| first record of pseudorabies in feral swine in nebraska. | in 2007, two new populations of feral swine were discovered in nance and valley counties, nebraska, usa. necropsies and serologic testing was done on two individuals from the nance county herd. results indicated that a lactating sow had positive antibodies for pseudorabies virus (prv). investigations conducted by nebraska game and parks commission law enforcement division confirmed that the infected individual was transported illegally to nebraska, usa, from texas, usa. all domestic swine herds ... | 2009 | 19617504 |
| assessment of the cell-mediated immunity induced by alphavirus replicon-vectored dna vaccines against classical swine fever in a mouse model. | we have previously shown that an alphavirus replicon-vectored dna vaccine (psfv1cs-e2) encoding the e2 glycoprotein of classical swine fever virus (csfv) completely protected the immunized pigs from lethal challenge. these animals developed only low or moderate level viral-specific antibody titers before challenge, implying that cell-mediated immunity (cmi) probably played an important role in the protective immunity against csfv conferred by the dna vaccine. in this study, the cmi induced by ps ... | 2009 | 19150136 |
| fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and fc portion of human igg1 provides a marked resistance against pseudorabies virus infection to transgenic mice. | nectin-1 is a ca2+-independent ig-like cell-cell adhesion molecule and an alphaherpesvirus receptor that binds to virion glycoprotein d by the first ig-like domain. we have investigated the antiviral potentials of soluble forms of porcine nectin-1 to prv infection by generating transgenic mice expressing different types of fusion protein. previously, we reported that mice transgenic for a chimera that carried the entire ectodomain of porcine nectin-1 fused to the fc portion of porcine igg1 were ... | 2009 | 19161552 |
| a multiplex pcr for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. | a multiplex pcr (mpcr) assay was developed and evaluated for its ability to simultaneously detect multiple viral infections of swine. specific primers were designed for each of the following four dna or rna viruses: porcine circovirus type 2 (pcv2), porcine parvovirus (ppv), pseudorabies virus (prv), and porcine reproductive and respiratory syndrome virus (prrsv). each target produced a specific amplicon with a size of 353 bp (pcv2), 271 bp (ppv), 194 bp (prv), or 434 bp (prrsv). the assay was s ... | 2009 | 19214728 |
| the role of spatial factors in the success of an aujeszky's disease eradication programme in a high pig density area (northeast spain, 2003-2007). | we tested the role of several spatial variables on the risk of a sow herd being aujeszky's disease virus (adv) seropositive in certain areas of north eastern spain and during different periods of the eradication programme. distance to the nearest slaughterhouse, distance to the nearest conventional road and number of adv serologically positive sows and adv serologically positive fattening pigs within different distances (1000, 1500 and 2000 m) of each sow herd, were included in a hierarchical ba ... | 2009 | 19631999 |
| cloning, in vitro expression, and bioactivity of interleukin-18 isolated from a domestic porcine breed found in henan. | to evaluate the effects of recombinant porcine interleukin-18 (rpil-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine il-18 (pil-18) isolated from a domestic big-white porcine breed found in the henan province (hn) was cloned using a reverse transcriptase-pcr. the cloned hn pil-18 contained an orf of 579 base pairs encoding a 192-amino-acid precursor protein. the amino acid sequence of hn pil-18 was compared with all the other pil-18 amino acid sequences a ... | 2009 | 19732142 |
| pseudorabies virus glycoprotein b can be used to carry foot and mouth disease antigens in dna vaccination of pigs. | to evaluate the feasibility of using pseudorabies virus (prv) glycoprotein b (gb) as a carrier of foot and mouth disease virus (fmdv) antigens in dna immunization, fmdv b- and t-cell epitopes were inserted either between the two b-cell epitopes of the n-term subunit of prv-gb (bt-prv-gb-n-term construct) or within the b-cell epitope of the c-term subunit of prv-gb (bt-prv-gb-c-term construct). two animal experiments were performed, each with three injections of plasmids 2 weeks apart, followed b ... | 2009 | 19103226 |
| rapid pre-clinical detection of classical swine fever by reverse transcription loop-mediated isothermal amplification. | the usefulness of reverse transcription loop-mediated isothermal amplification (rt-lamp) for rapid pre-clinical detection of classical swine fever virus (csfv) infection was evaluated. the rt-lamp reaction could be finished in 60 min under isothermal condition at 65 degrees c by employing a set of four primers targeting the 5' untranslated region of csfv. the rt-lamp assay of csfv showed higher sensitivities than that of rt-pcr, with a detection limit of 5 copies per reaction. no cross-reactivit ... | 2009 | 19103283 |
| different replication characteristics of historical pseudorabies virus strains in porcine respiratory nasal mucosa explants. | different alphaherpesviruses, including pseudorabies virus (prv), are able to cross the basement membrane barrier in nasal respiratory epithelium. as a first step in investigating this invasion process, a detailed quantitative analysis system was set up to determine the kinetics of horizontal and vertical virus spread in nasal explants, using the virulent prv strain 89v87. plaque latitudes, total depths, depths measured from the basement membrane and volumes were determined at 0, 12, 24 and 36h ... | 2009 | 19111405 |
| the relationship between infectious and non-infectious herd factors with pneumonia at slaughter and productive parameters in fattening pigs. | this paper explores the relationship between infectious and non-infectious herd factors with the occurrence of pneumonia at slaughter and productive parameters in fattening pigs on 39 fattening herds. a questionnaire was used to obtain environmental and management factors (non-infectious factors). blood samples and lungs were obtained from 35 pigs in each herd at slaughter. serological testing was performed for antibodies against three respiratory pathogens (infectious factors): porcine reproduc ... | 2009 | 18023215 |
| characterization and virus-induced expression profiles of the porcine interferon-omega multigene family. | interferon-omega is a member of the type i interferon family. in this work, 8 functional porcine interferon-omega genes and 4 pseudogenes present on porcine chromosome 1 were identified in the porcine genome database by blast scanning. their genetic and genomic characteristics were investigated using bioinformatics tools. then the poifn-omega functional subtype genes were isolated and expressed in bhk-21 cells. the poifn-omega subtypes possessed about 10(4) to 10(5) units of antiviral activity p ... | 2009 | 19792998 |
| [c3d-m28 enhanced dna vaccination induced humoral immune response to glycoprotein c of pseudorabies virus]. | we studied the immunogenicity of pseudorabies virus gc dna vaccination by fusing the murine complement c3d receptor binding domain. first, pseudorabies virus gc gene was linked to four copies of c3d receptor binding domain (m284), and then cloned into the vector pcdna3.1 to construct the recombinant plasmid sgc-m284. through the experiment of immunized balb/c mice, we found that the enzyme linked immunosorbent assay (elisa) antibody titer for sgc-m284 was 17-fold higher than that for sgc alone, ... | 2009 | 19835138 |
| whole-genome analysis of pseudorabies virus gene expression by real-time quantitative rt-pcr assay. | pseudorabies virus (prv), a neurotropic herpesvirus of pigs, serves as an excellent model system with which to investigate the herpesvirus life cycle both in cultured cells and in vivo. real-time rt-pcr is a very sensitive, accurate and reproducible technique that can be used to detect very small amounts of rna molecules, and it can therefore be applied for analysis of the expression of herpesvirus genes from the very early period of infection. | 2009 | 19852823 |
| pseudorabies virus infection alters neuronal activity and connectivity in vitro. | alpha-herpesviruses, including human herpes simplex virus 1 & 2, varicella zoster virus and the swine pseudorabies virus (prv), infect the peripheral nervous system of their hosts. symptoms of infection often include itching, numbness, or pain indicative of altered neurological function. to determine if there is an in vitro electrophysiological correlate to these characteristic in vivo symptoms, we infected cultured rat sympathetic neurons with well-characterized strains of prv known to produce ... | 2009 | 19876391 |
| global transcriptional response of pig brain and lung to natural infection by pseudorabies virus. | pseudorabies virus (prv) is an alphaherpesviruses whose native host is pig. prv infection mainly causes signs of central nervous system disorder in young pigs, and respiratory system diseases in the adult. | 2009 | 19948073 |
| comparison of different prime-boost regimes with dna and recombinant orf virus based vaccines expressing glycoprotein d of pseudorabies virus in pigs. | both dna and orf virus (orfv; parapox virus) based vaccines have shown promise as alternatives for conventional vaccines in pigs against pseudorabies virus (prv) infection causing aujeszky's disease. in the present study we evaluated the efficacy of different prime-boost regimes in pigs in terms of immunogenicity and protection against challenge infection with prv. the different prime-boost regimes consisted of the homologous prime-boost regimes (dna followed by dna or orfv followed by orfv) and ... | 2010 | 20018271 |
| pseudorabies virus us3-mediated inhibition of apoptosis does not affect infectious virus production. | preventing apoptosis during the early stages of infection of a host cell is generally thought to result in a higher yield of progeny virus. the us3 protein kinase of pseudorabies virus (prv) and herpes simplex virus (hsv) is able to protect infected cells from apoptosis, which may be one of the reasons why both us3null prv and us3null hsv replicate to lower virus titres in several cell types. however, such potential correlation between the higher amount of apoptosis in us3null virus-infected cel ... | 2010 | 20053819 |
| evaluation of humoral and antigen-specific t-cell responses after vaccination of pigs against pseudorabies in the presence of maternal antibodies. | in this study the influence of maternal immunity against pseudorabies virus (prv) on the development of humoral and t-cell mediated immune (cmi) responses was investigated under the experimental condition. pigs born to immune sows were vaccinated with ge-deleted vaccine according to five different schedules. peripheral blood mononuclear cells (pbmc), collected after vaccination, were used for prv-induced lymphocyte proliferation assay (lpa). antibodies to the gb and ge of prv in serum were deter ... | 2010 | 20153939 |
| multiplex nested rt-pcr for the detection of porcine enteric viruses. | porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine group a rotavirus (prv-a) are major viruses causing enteric diseases of piglets. a multiplex nested reverse transcription polymerase chain reaction (multiplex nested rt-pcr) was developed for the detection of these viruses in field samples from piglets with diarrhea. a mixture of (1) three external pairs of primers, yielding in the amplification step two different amplicons with sizes of 950 bp and 31 ... | 2010 | 20170679 |
| interferon-gamma secretion and proliferative responses of peripheral blood mononuclear cells after vaccination of pigs against aujeszky's disease in the presence of maternal immunity. | the development of t-cell responses in pigs vaccinated against aujeszky's disease in the presence of maternal-derived antibodies (mda) was examined. the aim of study was to evaluate the influence of mda on the postvaccinal t-cell responses and optimization vaccination protocols in mda-positive pigs. pigs born to immune sows were vaccinated at different ages against aujeszky's disease virus (adv). for estimation of t-cell responses the lymphocyte proliferation and interferon (ifn)-gamma and inter ... | 2010 | 20180849 |
| cytokine protein expression levels in tracheobronchial lymph node homogenates of pigs infected with pseudorabies virus. | pseudorabies virus (prv) is a neurotropic alphaherpesvirus that produces fatal encephalitis in newborn pigs, respiratory disorders in fattening pigs, and reproductive failure in sows. following primary infection of the respiratory tract, prv can develop into a systemic infection with dispersion of the virus via the lymphatic system that involves mononuclear cells in tracheobronchial lymph nodes (tblns). the objectives of the present study were to evaluate the pathogenesis and to determine the ea ... | 2010 | 20219878 |
| characterization of pseudorabies virus of wild boar origin from europe. | pseudorabies virus (prv) infections appear to be more widely distributed in the european wild boar (sus scrofa) population than assumed. in europe, attempts to isolate and characterize the causative agents have been limited so far. we therefore collected and examined a total of 35 prv isolates obtained from wild boar or hunting dogs in germany, france, spain, italy, slovakia and hungary between 1993 and 2008. restriction enzyme analysis of genomic dna using bamhi showed that all isolates, except ... | 2010 | 20223047 |
| molecular cloning and functional characterization of porcine stimulator of interferon genes (sting). | the stimulator of interferon genes (sting) of human/mouse has been identified recently as an adaptor that links virus-sensing receptors to interferon regulatory factor 3 (irf3) activation. here we report the cloning and characterization of porcine sting (posting). the full-length posting cdna sequence encodes 378 amino acids and contains one endoplasmic reticulum (er) retention motif, rar. phylogenetic analysis revealed that posting, together with bovine sting, is more closely related to the hum ... | 2010 | 20346968 |
| isolation and characterization of the first chinese strain of porcine teschovirus-8. | investigations were carried out to identify the causal agent of acute diarrhea, respiratory distress, and death of pigs on a swine farm in jilin province, northern china. only porcine teschovirus (ptv, designated as ptv-8 jilin/2003) was isolated from samples of organs. the presence of ptv was confirmed by the production of a specific cytopathic effect on susceptible cells and by the results of the immunoperoxidase monolayer assay (ipma), polymerase chain reaction, and electron microscopy. other ... | 2010 | 20362007 |
| porcine small intestinal epithelial cell line (ipec-j2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics. | previous studies of epithelial immune responses to rotavirus infection have been conducted in transformed cell lines. in this study, we evaluated a non-transformed porcine jejunum epithelial cell line (ipec-j2) as an in-vitro model of rotavirus infection and probiotic treatment. cell-culture-adapted porcine rotavirus (prv) osu strain, or human rotavirus (hrv) wa strain, along with lactobacillus acidophilus (la) or lactobacillus rhamnosus gg (lgg) were used to inoculate ipec-j2 cells. la or lgg t ... | 2010 | 20373994 |
| variation of aujeszky's disease viruses in wild swine in usa. | in the united states of america, aujeszky's disease (pseudorabies) has been eradicated from all domestic swine. some re-emergence of infection occurred as vaccine use diminished. sporadic outbreaks have also occurred because of the reservoir of infection in feral swine that have spread across the southern two-thirds of the country and hawaii. in order to be able to understand the origins of re-emerging virus, sequence analysis of variable genes in pseudorabies virus (prv) has been used to differ ... | 2010 | 20444559 |
| role of the ul41 protein of pseudorabies virus in host shutoff, pathogenesis and induction of tnf-α expression. | the vhs (virion host shutoff) is highly conserved in alphaherpesvirus, including pseudorabies virus (prv). in an attempt to explore the function of vhs of prv, we constructed and characterized a mutant virus (δ41). in the absence of vhs activity, δ41 mutant is highly attenuated in mice model and the lethality is correlated with the virus dissemination in neural tissues. as with herpes simplex virus type 1 (hsv-1), the prototype virus of alphaherpesvirus, the pronounced decrease in cellular prote ... | 2010 | 20448414 |
| swine virus-specific cytotoxic cells producing ifngamma under different conditions of virus antigenic stimulation. | flow cytometry is a useful tool to determine both the phenotype and function of immune cells during vaccination and viral infection. since cytokine release is a hallmark of cell activation, detection of intracellular interferon (ifn)gamma in cytotoxic cd8alpha+ cells under physiological conditions and after aujeszky's disease virus (adv)- or porcine circovirus type 2 (pcv2)-swine interaction was carried out. blood samples were collected from healthy 10-month-old pigs, adv- or pcv2-vaccinated pig ... | 2010 | 20512626 |
| selected swine viral pathogens in indoor pigs in spain. seroprevalence and farm-level characteristics. | a serosurvey on porcine reproductive and respiratory syndrome virus (prrsv), swine influenza virus (siv), aujeszky's disease virus ge protein (adv ge), porcine parvovirus (ppv) and porcine circovirus type 2 (pcv2) was carried out in spanish pig herds. the serosurvey consisted of two studies. first, a retrospective study assessed the proportion of seropositive boar, sow and fattening pig herds and their seroprevalences to prrsv, siv, adv ge and ppv from 2003 to 2005 and to pcv2 from 2000 to 2005. ... | 2010 | 20569418 |
| vectored vaccines to protect against prrsv. | prrsv is the causative agent of the most important infectious disease affecting swine herds worldwide, producing great economic losses. commercially available vaccines are only partially effective in protection against prrsv. moreover, modified live vaccines may allow virus shedding, and could revert generating virulent phenotypes. therefore, new efficient vaccines are required. vaccines based on recombinant virus genomes (virus vectored vaccines) against prrsv could represent a safe alternative ... | 2010 | 20600388 |
| loop-mediated isothermal amplification for rapid detection and differentiation of wild-type pseudorabies and gene-deleted virus vaccines. | a loop-mediated isothermal amplification (lamp) assay was developed specifically for detection and differentiation of pseudorabies virus (prv). one group of primers was designed to detect wild-type strains (i.e., strains with the ge gene) and the other group of primers was designed to detect both prv ge-vaccine and wild-type strains (i.e., strains with the gg gene and with or without the ge gene). after amplification by bst enzyme at a constant temperature of 65 degrees c, a laddering of bright ... | 2010 | 20691214 |
| establishment of a multiplex rt-pcr assay to detect different lineages of swine h1 and h3 influenza a viruses. | classical swine h1n1, emerging european avian-like h1n1 and human-like h3n2 lineages are co-circulating in the swine population in china. the reverse transcriptase polymerase chain reaction (rt-pcr) assay is an effective method for use in influenza surveillance. in this study, a multiplex rt-pcr method was developed for simultaneous identification of hemagglutinin (ha) genes derived from the three lineages of swine influenza viruses. three primer sets were designed and aimed specifically at ha g ... | 2010 | 20700759 |
| [construction of an infectious clone of pseudorabies virus strain zj genome maintained as a bacterial artificial chromosome]. | pha2 plasmid sequence,with bacterial artificial chromosome(bac) vector and the gfp expression cassette, was introduced into the ul23(tk) gene of pseudorabies virus(prv)strain zj by homologous recombination,and the recombinant prv (rprv-ha2) was confirmed and isolated by plaque purification. the circular genome of rprv-ha2 was electroporated into escherichia coli strain dh10b and then the prv bac (pprv) was recovered. the transfection of pprv into veroe6 cells resulted in productive infection. th ... | 2010 | 20836388 |
| development of a porcine epidemic diarrhea virus m protein-based elisa for virus detection. | a membrane (m), protein-based elisa was developed to detect porcine epidemic diarrhea virus (pedv). the m gene of pedv was expressed in escherichia coli. the purified recombinant m protein was used to immunize rabbits to generate a polyclonal antibody. immunofluorescence analysis indicated that the anti-pedv-m antibody reacted with pedv-infected cells. the antibody was utilized to develop an indirect elisa to detect pedv. other viruses, porcine transmissible gastroenteritis coronavirus, avian in ... | 2010 | 20882317 |
| comparison of the protective efficacy of recombinant adenoviruses against classical swine fever. | classical swine fever (csf), which is caused by classical swine fever virus (csfv), is a highly contagious and often fatal swine disease that is responsible for significant losses to the swine industry worldwide. previously, we demonstrated that pigs immunized with a recombinant adenovirus (radv-e2) expressing the e2 glycoprotein of csfv were protected against virulent csfv; however, a few pigs showed a short-term fever and occasional pathological changes. to enhance the efficacy of the vaccine, ... | 2010 | 20923683 |
| the effect of houttuynia cordata injection on pseudorabies herpesvirus (prv) infection in vitro. | pseudorabies herpesvirus (prv) belongs to the alphaherpesvirinae. piglets infected with prv die within a few days. development of effective antiviral agents is one alternative or complementary method to prevent prv infection. houttuynia cordata thunb. (saururaceae), h. cordata, a traditional chinese medicine, is often used to relieve lung abnormal symptoms, infectious disease, refractory hemoptysis and malignant pleural effusion in china. | 2010 | 20942608 |
| recombinant pseudorabies virus expressing p12a and 3c of fmdv can partially protect piglets against fmdv challenge. | one of the crucial factors for evaluation of an effective genetically engineered vaccine is whether susceptible animals are protected from virus challenge after vaccination. in this study, a recombinant pseudorabies virus (prv-p12a3c) that expressed capsid precursor polypeptide p12a and nonstructural protein 3c of foot-and-mouth disease virus (fmdv) was used as a vaccine. the expression of p12a3c and its immunogenicity and protective efficacy against fmdv challenge were measured. humoral and cel ... | 2010 | 20947111 |
| a novel rt-lamp assay for rapid and simple detection of classical swine fever virus. | a simple and rapid assay for the detection of classical swine fever virus (csfv) was established using reverse transcription loop-mediated isothermal amplification (rt-lamp). this study describes the amplification of the genomic rna of csfv under isothermal conditions (63 °c) within one hour, using a set of six primers (two outer primers, two inner primers and two loop primers). this rt-lamp assay showed 100-fold higher sensitivity than the standard rt-pcr method and identified eighteen addition ... | 2010 | 20960285 |
| spontaneous aujeszky's disease (pseudorabies) in european wild boars (sus scrofa) in the federal state of brandenburg, germany. | in this report two cases of spontaneous aujeszky's disease (ad, syn. pseudorabies) in european wild boars (sus scrofa), in the federal state of brandenburg, germany, are described. both animals displayed severe central nervous disturbances, including loss of fear of humans, disorientation, and tremors of head and limbs, and were shot by hunters for laboratory exclusion of rabies. the main finding in the well nourished, juvenile (approx. 7-8 months) animals was a non-suppurative panencephalitis c ... | 2010 | 21038806 |
| molecular cloning, expression and antiviral activity of porcine interleukin-29 (poil-29). | human interleukin-29 (il-29) is a recently discovered cytokine displaying antiviral activity against a broad spectrum of viruses, designated as interferon (ifn)-λ1. we here report the molecular cloning, expression and antiviral activity of porcine il-29 (poil-29). the full-length poil-29 cdna sequence encoded 191 amino acids with a 19 amino acid signal peptide. sequence alignments showed that poil-29 had amino acid sequence similarity to wolf (76%), bear (75%), horse (75%), orangutan (73%), huma ... | 2010 | 21078342 |
| green propolis phenolic compounds act as vaccine adjuvants, improving humoral and cellular responses in mice inoculated with inactivated vaccines. | adjuvants play an important role in vaccine formulations by increasing their immunogenicity. in this study, the phenolic compound-rich j fraction (jfr) of a brazilian green propolis methanolic extract stimulated cellular and humoral immune responses when co-administered with an inactivated vaccine against swine herpesvirus type 1 (suhv-1). when compared to control vaccines that used aluminium hydroxide as an adjuvant, the use of 10 mg/dose of jfr significantly increased (p < 0.05) neutralizing a ... | 2010 | 21120362 |
| detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the ns1 gene. | a taqman-based real-time polymerase chain reaction (pcr) assay was devised for the detection of porcine parvovirus (ppv). two primers and a taqman probe for the non-structural protein ns1 gene were designed. the detection limit was 1 x 10² dna copies/μl, and the assay was linear in the range of 1 x 10² to 1 x 10⁹ copies/μl. there was no cross-reaction with porcine circovirus 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), pseudorabies virus (prv), classical swine fever vir ... | 2010 | 21126330 |
| reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus. | transmissible gastroenteritis virus (tgev) is the causative agent of porcine transmissible gastroenteritis, and sensitive detection methods are required for preventing the disease. in this article, reverse transcription-loop-mediated isothermal amplification (rt-lamp) was developed to detect tgev. three pairs of primers targeting the nucleocapsid (n) gene of tgev were synthesized and used in the rt-lamp. the optimization, sensitivity, and specificity of the rt-lamp were evaluated. our results sh ... | 2010 | 21127872 |
| a differential elisa based on recombinant immunodominant epitopes of the ge gene of shv-1 in a baculovirus-insect cell system to discriminate between pigs infected naturally with pseudorabies and vaccinated pigs. | in the present study, the fragment corresponding to the immunodominant epitopes of the ge gene (gepi) from the cl15 argentinean strain of pseudorabies virus was expressed successfully in a baculovirus-insect cell system that contained the m6 gene of bluetongue virus, which encodes the ns1 nonstructural protein. this protein has the ability to polymerize into highly immunogenic tubules inside infected cells that can be purified at large quantities by ultracentrifugation. previously, the ns1 prote ... | 2010 | 21168443 |
| herpesviruses carrying a brainbow cassette reveal replication and expression of limited numbers of incoming genomes. | whether all the infectious herpesvirus particles entering a cell are able to replicate and/or express their genomes is not known. here, we developed a general method to determine the number of viral genomes expressed in an infected cell. we constructed and analysed fluorophore expression from a recombinant pseudorabies virus (prv263) carrying a brainbow cassette (cre-conditional expression of different fluorophores). using three isogenic strains derived from prv263, each expressing a single fluo ... | 2010 | 21266996 |
| development and validation of reverse transcription loop-mediated isothermal amplification for detection of prrsv. | to establish a reverse-transcription loop-mediated isothermal amplification (rt-lamp) method for rapid detection of porcine reproductive and respiratory syndrome virus (prrsv), four primers specific to six regions of the n gene were designed. after amplification in an isothermal water bath for 1 h, samples containing prrsv generated the expected ladder-like products while porcine parvovirus, porcine circovirus, classic swine fever virus, pseudorabies virus, and swine testis cells generated no pr ... | 2010 | 19911264 |
| development and evaluation of rapid detection of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (rt-lamp). | a one-step reverse transcription loop-mediated isothermal amplification (rt-lamp) was developed for the detection of classical swine fever virus (csfv) and a set of primers designed based on the e2 gene reference sequences of the csfv. the assay was optimized to amplify csfv rna by incubation at 63 degrees c for 50 min. the rt-lamp amplification products had a ladder-like appearance when electrophoresed on an agarose gel. the rt-lamp assay showed higher sensitivity than the conventional rt-pcr, ... | 2010 | 19931578 |
| characterization of the cytokine and maturation responses of pure populations of porcine plasmacytoid dendritic cells to porcine viruses and toll-like receptor agonists. | plausible representatives of plasmacytoid dendritic cells (pdcs) in pigs have been characterized as being cd4(hi)cd172(lo). due to their paucity in blood, we utilized novel fluorescent-activated cell sorting procedures to isolate them from pbmc. the resultant subset was greater than 98% homogeneous in regards to the selected phenotype and contained the preponderance of individuals secreting ifn-alpha after exposure to a known stimulant, transmissible gastroenteritis virus (tgev). in addition to ... | 2010 | 19939462 |
| molecular cloning and functional characterization of porcine dna-dependent activator of ifn-regulatory factors (dai). | the dna-dependent activator of ifn-regulatory factors (dai) is a recently identified dna sensor for intracellular dna that triggers a signal for the production of type i ifn. here we report the cloning and characterization of porcine dai (podai). the full-length of podai encodes 439 amino acids, contains two n-terminal dna-binding domains and shows similarity to mouse, rat, dog, monkey, human, horse and cattle counterparts ranging from 44% to 67%. podai mrna expression was mainly detected in spl ... | 2010 | 19850075 |
| prime-boost immunization with ha/c3d dna followed by a recombinant pseudorabies virus boost enhanced protective immunity against h3n2 swine influenza virus in mice. | dna and recombinant virus vaccines against swine influenza virus (siv) have been pursued with promising results, but induce poor immunogenicity. this study evaluated the effects of a vaccine regimen in mice including priming with three dna vaccines expressing soluble ha (sha), complete ha (tmha), or sha fused with three copies murine c3d (sha-mc3d3) and boosting with recombinant pseudorabies virus expressing ha (rprv-ha). immune responses were monitored by elisa, hi assays, and virus neutralizat ... | 2010 | 19850311 |
| molecular epidemiology of brazilian pseudorabies viral isolates. | pseudorabies is a disease caused by pseudorabies virus (prv) and is responsible for considerable economic losses in the swine industry. the objective of this work was to use molecular epidemiology as a tool to facilitate the study of prv outbreaks in brazil. the standard prv strain shope, the vaccine strain bartha and isolates from the south and the southeast regions of brazil, were amplified for ge and gc partial genes by pcr. results indicated that brazilian prv isolates are grouped in two clu ... | 2010 | 19828266 |
| expression and snp association analysis of porcine fbxl4 gene. | as a kind of e3 ligase, the product of fbxl4 gene belongs to a member of fbls which is the biggest eukaryotic subfamily of f-box proteins, it can recognize some substrate through particular protein-protein interaction domains. to investigate its functions, the polymorphism and association analysis was analyzed. the partial cdna of porcine fbxl4 with 2384 bp long was first cloned; the deduced protein comprises a conserved f-box domain at position from the 277th to 332nd amino acid. the phylogenet ... | 2010 | 19768576 |
| glycoprotein g from pseudorabies virus binds to chemokines with high affinity and inhibits their function. | pseudorabies virus (prv), also known as suid herpesvirus, is the aetiological agent of aujeszky's disease in swine. in other animals, except higher-order primates, prv infection is often fatal. the mechanisms of prv pathogenesis and immune modulation are largely unknown. prv codes for 11 glycoproteins. among them, glycoprotein g (gg) is the most abundant prv protein found in the supernatant of prv-infected cell cultures. prv-gg has low amino acid sequence similarity with gg from other animal alp ... | 2010 | 19776237 |
| immunohistochemical detection of aetiological agents of proliferative and necrotizing pneumonia in italian pigs. | proliferative and necrotizing pneumonia (pnp) is a form of interstitial pneumonia that occurs in weaning and post-weaning pigs. pnp is characterized by hypertrophy and hyperplasia of type ii pneumocytes and coagulative necrosis and granular debris within alveolar spaces. canadian and european studies suggest that the porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) are the main causes of the disease, but aujezsky's disease virus (adv) and swine inf ... | 2010 | 19631945 |
| effect of haemolysis and repeated freeze-thawing cycles on wild boar serum antibody testing by elisa. | abstract: | 2011 | 22087883 |
| Seroprevalence of porcine reproductive and respiratory syndrome, Aujeszky's disease, and porcine parvovirus in replacement gilts in Thailand. | The present study investigated the seroprevalence of porcine reproductive and respiratory syndrome virus, Aujeszky's disease virus (ADV), and porcine parvovirus (PPV) in replacement gilts from selected five swine herds in Thailand. The study consisted of three parts. First, a retrospective data analysis on the seroprevalence of porcine reproductive and respiratory syndrome virus (PRRSV) and ADV glycoprotein I (gI) in gilts, sows, boars, nursery, and fattening pigs in five herds (n?=?7,030). Seco ... | 2011 | 22068636 |
| Loop-mediated isothermal amplification for detection of porcine circovirus type 2. | ABSTRACT: | 2011 | 22044506 |
| microbial ecology of swine farms and prrs vaccine vaccination strategies. | the present study investigated the microbial ecology and vaccination strategies against porcine reproductive and respiratory syndrome (prrs) in field condition. four representative farms with a history of prrs were included in this study. over the almost 3-year period, the average detection rate was 68.9%, making prrsv the first most frequently detected virus, followed by porcine circovirus type 2 (pcv2) (64.2%), pseudorabies virus (prv) (11.03%) and classical swine fever virus (csfv) (4.41%). s ... | 2011 | 22014373 |
| magnetic beads-based electrochemical immunosensor for detection of pseudorabies virus antibody in swine serum. | a novel magnetic electrochemical immunosensor has been developed for the detection of pseudorabies virus antibody in swine serum. the magnetic glass carbon electrode was fabricated to manipulate magnetic beads for the direct sensing applications. magnetic beads were employed as the platforms for the immobilization and immunoreaction process, and gold nanoparticles were chosen as electroactive labels for the electrochemical detection. the parameters concerning the assay strategy were carefully in ... | 2011 | 22099683 |
| A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses. | Alphaherpesviruses are widespread in the human population, and include herpes simplex virus 1 (HSV-1) and 2, and varicella zoster virus (VZV). These viral pathogens cause epithelial lesions, and then infect the nervous system to cause lifelong latency, reactivation, and spread. A related veterinary herpesvirus, pseudorabies (PRV), causes similar disease in livestock that result in significant economic losses. Vaccines developed for VZV and PRV serve as useful models for the development of an HSV ... | 2011 | 22022263 |
| Electroporation improves the immune response induced by a DNA vaccine against pseudorabies virus glycoprotein B in pigs. | This study was performed to determine whether electroporation can be used to enhance the efficacy of a DNA vaccine against pseudorabies virus (PrV) in pigs. Immune responses to PrV were measured in pigs following a single intramuscular injection of plasmids encoding PrV glycoprotein B, with or without electroporation. Plasmid injection coupled with electroporation increased production of specific antibodies against PrV and peripheral blood mononuclear cells proliferated in response to stimulatio ... | 2011 | 22051145 |
| Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes. | Our aim was to analyse the transcription levels of the three non-classical class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex in various tissues and conditions and to compare them to the transcription levels of classical class Ia genes. Twenty-five adult tissues from two pig breeds, pig renal PK15 cells infected with the Pseudorabies virus, and peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide or a mixture of phorbol myristate acetate an ... | 2011 | 21906102 |
| detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification. | a loop-mediated isothermal amplification (lamp) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (pcv1) in commercial swine vaccines. this method was highly specific for pcv1. no cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. the analytical sensitivity of the lamp for pcv1 dna was 10 copies/μl in the case of ... | 2011 | 21948286 |
| oral administration of salmonella enterica serovar typhimurium expressing swine interleukin-18 induces th1-biased protective immunity against inactivated vaccine of pseudorabies virus. | enhancing and/or modulating innate and adaptive immunity by cytokines appears to be greatly useful to provide effective protective immunity against infectious diseases. however, an effective delivery system for mass administration in livestock industry is needed because of limitations such as cost, labor, time, and protein stability. here the immunomodulatory functions of swine interleukine-18 (swil-18), known as ifn-γ-inducing factor (igif), were evaluated in a vaccination model of pseudorabies ... | 2011 | 21940117 |
| pseudorabies virus infection in oklahoma hunting dogs. | pseudorabies is caused by suid herpesvirus 1, a member of the alphaherpesvirinae subfamily. although pigs are the natural host of pseudorabies virus (prv), the virus has a broad host range and may cause fatal encephalitis in many species. the united states obtained prv-free status in 2004 after the virus was eradicated from domestic swineherds, but the virus is still present in feral swine populations. the current report describes prv infection in 3 dogs that were used to hunt feral swine. the d ... | 2011 | 21908347 |
| detection and molecular characterization of suid herpesvirus type 1 in austrian wild boar and hunting dogs. | aujeszky's disease (ad), caused by suid herpesvirus type 1 (suhv-1), is an economically important disease in domestic swine. thus, rigorous control programmes have been implemented and consecutively ad in domestic swine was successfully eradicated in many countries, including austria. however, suhv-1 continues to thrive in wild boar populations, as indicated by high seroprevalences in a number of european countries and by occasional cases of ad in hunting dogs. for the first time, suhv-1 was det ... | 2011 | 22264387 |
| characterization of the porcine p65 subunit of nf-?b and its association with virus antibody levels. | nf-?b p65 subunit plays important roles in controlling both innate and adaptive immunity. here we report the characterization of porcine nf-?b p65 subunit (pp65). pp65 shows high similarity to other mammalian counterparts. pp65 mrna expression was mainly observed in lung, spleen, liver and small intestine. furthermore, overexpression of pp65 activates nf-?b in porcine endothelial cell line piec, porcine alveolar macrophages cell line 3d4/21 and porcine primary fetal fibroblasts. a cooh-terminal ... | 2011 | 21269694 |
| development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus. | in this study, a reverse transcription loop-mediated isothermal amplification (rt-lamp) was developed for detection of porcine epidemic diarrhea virus (pedv). six primers were designed to amplify the nucleocapsid (n) gene of pedv. the optimization, sensitivity, and specificity of the rt-lamp were investigated. the results showed that the optimal reaction condition for rt-lamp amplifying pedv n gene was achieved at 63°c for 50 min. the rt-lamp assay was more sensitive than gel-based rt-pcr and en ... | 2011 | 21286798 |
| cpg oligodeoxynucleotide synergizes innate defense regulator peptide for enhancing the systemic and mucosal immune responses to pseudorabies attenuated virus vaccine in piglets in vivo. | oligonucleotides containing cpg motifs (cpg odn) are strong adjuvants for humoral and cellular immune responses in mice, and innate defense-regulator peptides (idrs) are known to facilitate the uptake of antigens into antigen presenting cells (apcs), but data on synergistic effects of cpg and idrs in piglets are scarce. in this report, the combination of porcine-specific cpg odn and hh2 (a kind of idr which was selected for its better synergy with cpg odn) was used as immunoadjuvant to enhance t ... | 2011 | 21310256 |
| a long-term serological survey on aujeszky's disease virus infections in wild boar in east germany. | summarybetween 1985 and 2008, a total of 102 387 wild boar sera originating from eastern germany covering an area of 108 589 km2 were tested for the presence of aujeszky's disease virus (adv)-specific antibodies. from 1985 until 1991 and from 1992 until 2008, wild boar sera were exclusively investigated using either conventional seroneutralization assays (n=39 621) or commercial gb and full antigen elisas (n=62 766), respectively. spatial-temporal analysis revealed an increasing adv seroprevalen ... | 2011 | 21320372 |
| porcine teschovirus polioencephalomyelitis in western canada. | beginning in 2002, a small number of pig farms in western canada began reporting 4-7-week-old pigs with bilateral hind-end paresis or paralysis. low numbers of pigs were affected, some died, most had to be euthanized, and those that survived had reduced weight gains and neurological deficits. necropsies revealed no gross lesions, but microscopic lesions consisted of a nonsuppurative polioencephalomyelitis, most severe in the brain stem and spinal cord. the lesions were most consistent with a vir ... | 2011 | 21398466 |
| co-expression of host and viral micrornas in porcine dendritic cells infected by the pseudorabies virus. | micrornas are small non-coding rnas approximately 22 nt long that modulate gene expression in animals and plants. it has been recently demonstrated that herpesviruses encode mirnas to control the post-transcriptional regulation of expression from their own genomes and possibly that of their host, thus adding an additional layer of complexity to the physiological cross-talk between host and pathogen. the present study focussed on the interactions between porcine dendritic cells (dcs) and the pseu ... | 2011 | 21408164 |
| deletion of the virion host shut: off gene of pseudorabies virus results in selective upregulation of the expression of early viral genes in the late stage of infection. | a real-time rt-pcr technique was applied to evaluate the impact of deletion of the virion host shut-off (vhs) gene on the kinetics of pseudorabies virus gene expression. selective suppression of early gene transcripts by the viral ribonuclease occurs after 4h of infection; while vhs protein appears to act non-selectively on the transcripts belonging in different kinetic classes in the first 2h of infection. vhs protein disrupts the close correlation between the transcription kinetics of the imme ... | 2011 | 21466845 |
| differentiation of porcine reproductive and respiratory syndrome virus n protein using a virus-based elisa. | the bacterially expressed nucleocapsid (n) protein of porcine respiratory and reproductive syndrome virus (prrsv) was used as immunogen to generate a rabbit-derived polyclonal antibody. the immunoreactivity of the protein to the antibody was confirmed by western blot analysis. using prrsv, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, pseudorabies virus, and avian infectious bronchitis virus as coating antigens, a virus-based elisa was established. the polyclonal antibody ... | 2011 | 21529294 |
| making contact: rooting out the potential for exposure of commercial production swine facilities to feral swine in north carolina. | despite north carolina's long history with feral swine, populations were low or absent in eastern counties until the 1990s. feral swine populations have since grown in these counties which also contain a high density of commercial production swine (cps) facilities. sixteen of the highest swine producing u.s. counties also populated with feral swine are in north carolina. disconcertingly, since 2009, positive tests for exposure to swine brucellosis or pseudorabies virus have been found for feral ... | 2011 | 21598058 |
| growth, physicochemical properties, and morphogenesis of chinese wild-type prv fa and its gene-deleted mutant strain prv sa215. | abstract: background: prv fa is common in china and causes most of the pseudorabies in the pig industry. a prv sa215 strain with deleted ge, gi, and tk genes was constructed to develop a commercial attenuated live vaccine. however, the physicochemical properties, growth pattern, penetration kinetics, and morphogenesis of the prv sa215 and its parental prv fa strain are unclear. results: a series of experiments were conducted to characterize both strains and provide more information. prv fa and p ... | 2011 | 21639925 |
| experimental intracerebral vaccination protects mouse from a neurotropic virus by attracting antibody secreting cells to the cns. | in previous studies, we showed that intracerebrally (ic) immunized mice had antigen-specific antibodies (abs) in cerebrospinal fluid and could survive lethal doses of transneurally spreading viruses. to better understand the mechanisms behind this, immune responses in both the central nervous system (cns) and lymphoid organs following intracerebral immunization against pseudorabies virus (prv) were investigated by focusing on antibody secreting cells (ascs). ic immunized mice had significantly h ... | 2011 | 21645547 |
| effects of the polymorphisms of mx1, bat2 and cxcl12 genes on immunological traits in pigs. | it is necessary that genetic markers or biomarkers can be used to predict resistance towards a wide range of infectious diseases. in the present study, we estimated the potential markers and measured their relationship with heritabilities of a wide range of immune traits. polymorphisms in exon 13 of mx1, intron 25 of bat2 and intron 3 of cxcl12 were identified by sequencing, and the genotypes were analyzed by pcr-rflp in a resource population composed of 352 pure breed landrace piglets at days 0 ... | 2011 | 21667240 |
| immunogenic peptides from phage display libraries with potential of protecting mice against the pseudorabies virus. | phage display selection of combinatorial peptide libraries has demonstrated its almost unlimited potential in identifying binding ligands for many targets. the method shows promise for selection of immunogenic peptides against pathogens by antibodies. we have undertaken a study designed to select such mimics for one of the representatives of herpesviridae, the pseudorabies virus (prv), infecting pigs and causing severe neurological complications known as aujeszky's disease. by screening a 12mer ... | 2011 | 21764227 |
| pseudorabies virus infection in wild boars in japan. | in japan, most pig populations are now free from pseudorabies virus (prv) due to the recent success of an extensive eradication program. however, prv infection persists in japanese wild boars (sus scrofa leucomystax), representing another potential reservoir for the virus in japan. in this study, the seroprevalence of prv in wild boars captured in three different prefectures was ascertained. the virus-neutralization (vn) test showed that 6 of 173 (3%) were positive for vn antibody; glycoprotein ... | 2011 | 21757858 |
| pseudorabies virus in wild swine: a global perspective. | suid herpesvirus 1 (suhv1, syn. aujeszky's disease virus [adv] or pseudorabies virus [prv]), which belongs to the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus is the causative agent of aujeszky's disease (ad, pseudorabies), a notifiable disease, that causes substantial economic losses to the swine industry in countries, where ad is present. members of the family suidae (true pigs) are the only natural hosts for prv, although the virus can infect numerous other mammals ... | 2011 | 21837416 |
| establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species. | a sheep kidney-derived cell line, flk-n3, was successfully established after serial (>100) passages. persistent infection of this cell line with viruses and mycoplasma was not detected. the cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus, bov ... | 2011 | 21695582 |
| A novel recombinant pseudorabies virus expressing parvovirus VP2 gene: Immunogenicity and protective efficacy in swine. | Porcine parvovirus (PPV) VP2 gene has been successfully expressed in many expression systems resulting in self-assembly of virus-like particles (VLPs) with similar morphology to the native capsid. Here, a pseudorabies virus (PRV) system was adopted to express the PPV VP2 gene. | 2011 | 21679423 |
| Histopathological investigation in porcine infected with torque teno sus virus type 2 by inoculation. | ABSTRACT: BACKGROUND: Porcine torque teno sus virus (TTSuV) is a small icosahedral and non-enveloped virus which contains a single-stranded (ssDNA), circular and negative DNA genome and infects mainly vertebrates and is currently classified into the 'floating' genus Anellovirus of Circoviridae with two species. Viral DNA of both porcine TTSuV species has a high prevalence in both healthy and diseased pigs worldwide and multiple infections of TTSuV with distinct genotypes or subtypes of the same ... | 2011 | 22171963 |
| Survey of pleuritis and pulmonary lesions in pigs at abattoir with a focus on the extent of the condition and herd risk factors. | Cranioventral pulmonary consolidation (enzootic pneumonia-like lesions) and chronic pleuritis (CP) are common findings in slaughtered pigs. Pleural lesions involving dorsocaudal lobes are suggestive of pleuropneumonia due to Actinobacillus pleuropneumoniae. In this report the results of an abattoir survey of pleuritis and pulmonary lesions in pigs is presented with a focus on herd risk factors. A total of 4889 animals, ranging in age from 9 to 10months, from 48 batches of pigs belonging to an eq ... | 2011 | 22182431 |
| The design and recombinant protein expression of a consensus porcine interferon: CoPoIFN-a. | CoPoIFN-a is a recombinant non-naturally occurring porcine interferon-a (IFN-a). It was designed by scanning 17 porcine IFN-a nonallelic subtypes and assigning the most frequently occurring amino acid in each position. We used a porcine IFN-a (PoIFN-a) derived from domestic pig as a control. Both porcine IFN-a genes were introduced into yeast expression vector PpICZa-A and expressed in Pichia pastoris. The antiviral unit of these two IFN-as were assayed in MDBK, PK-15 and MARC-145 cells against ... | 2012 | 22088600 |
| A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs. | Multiple viral infections are common in pigs under intensive production conditions. All five of the viruses included in this study are associated with multifactorial diseases that cause significant economic losses in swine farming worldwide. The development is described of a novel multiple real-time PCR system based on the use of SYBR Green I that allows the simultaneous detection and differentiation of porcine circovirus 2 (PCV-2), porcine parvovirus (PPV), pseudorabies virus (PRV) and Torque t ... | 2012 | 22119629 |
| prevalence of antibodies to selected viral pathogens in wild boars (sus scrofa) in croatia in 2005-06 and 2009-10. | we determined prevalence of antibody to selected viral pathogens important for domestic pigs and livestock in 556 wild boar (sus scrofa) sera collected during 2005-06 and 2009-10 in four counties in croatia. these counties account for an important part of the croatian commercial pig production and have a high density of wild boars. samples were tested for antibodies to porcine parvovirus (ppv), aujeszky's disease virus (adv), porcine circovirus type 2 (pcv2), swine influenza virus, porcine respi ... | 2012 | 22247381 |
| wild boar: an increasing concern for aujeszky's disease control in pigs? | the goal of this study was describing the temporal evolution of aujeszky's disease virus (adv) contact prevalence among eurasian wild boar (sus scrofa) populations under different management regimes and contact likelihoods with domestic pigs. given the recent increase in wild boar abundance throughout europe, we hypothesized that wild boar contact with adv would remain stable in time even after significant reduction of adv prevalence in domestic pigs. | 2012 | 22251441 |
| a serosurvey for brucella suis, classical swine fever virus, porcine circovirus type 2, and pseudorabies virus in feral swine (sus scrofa) of eastern north carolina. | as feral swine (sus scrofa) populations expand their range and the opportunity for feral swine hunting increases, there is increased potential for disease transmission that may impact humans, domestic swine, and wildlife. from september 2007 to march 2010, in 13 north carolina, usa, counties and at howell woods environmental learning center, we conducted a serosurvey of feral swine for brucella suis, pseudorabies virus (prv), and classical swine fever virus (csfv); the samples obtained at howell ... | 2012 | 22493123 |
| prevalence of and risk factors associated with viral and bacterial pathogens in farmed european wild boar. | the aim of this study was to estimate in farmed european wild boars the prevalence of and risk factors associated with a range of common porcine viral and bacterial infections, namely, porcine parvovirus (ppv), porcine circovirus type 2 (pcv2), swine influenza virus (siv), aujeszky's disease virus (adv), classical swine fever virus (csfv), swine vesicular disease virus (svdv), coronavirus causing transmissible gastroenteritis (tgev), porcine reproductive and respiratory syndrome virus (prrsv), m ... | 2012 | 22516920 |
| preferential sexual transmission of pseudorabies virus in feral swine populations may not account for observed seroprevalence in the usa. | this paper compares the behavior of two competing models for the transmission of pseudorabies virus in feral swine in the usa. in first model, horizontal (non-sexual) density dependent transmission is the only transmission modality. in the second model, the only transmission modality is sexual transmission between mature males and females. the comparison of model behavior was carried out to test the hypothesis that preferential sexual transmission of prv in feral swine can account for the seropr ... | 2012 | 21962753 |
| effects of culling eurasian wild boar on the prevalence of mycobacterium bovis and aujeszky's disease virus. | worldwide, failure to eradicate a disease in livestock has sometimes been related to wildlife reservoirs of infection. we describe the effects of eurasian wild boar (sus scrofa) abundance reduction through increased culling on the prevalence of two chronic infectious diseases, tuberculosis (tb) and aujeszky's disease (ad), in a region of south-central spain (scs). the two infections studied responded differently to an approximately 50% reduction of wild boar abundance. wild boar tb prevalence re ... | 2012 | 22743215 |
| oral immunization of wild boar and domestic pigs with attenuated live vaccine protects against pseudorabies virus infection. | in domestic pigs strict control measures and the use of gene-deleted marker vaccines resulted in the elimination of pseudorabies virus (prv) infections in many parts of europe and north america. in free-roaming feral pigs and wild boar populations, however, serological surveys and monitoring in the americas, europe and north africa provided serological and virological evidence that prv is more widely distributed than previously assumed. thus, there is a constant risk of spillover of prv infectio ... | 2012 | 22832373 |
| nonsuppurative encephalitis in a dog. | a 4-year-old male german hunting terrier presented with tremor, dyspnea, trismus, spasms of the musculature of the larynx and pharynx, and hypothermia and subsequently died despite intensive clinical care. prior clinical signs included vomitus and diarrhea. microscopic examination of the brain revealed a multifocal nonsuppurative brain stem encephalitis; a few intralesional neurons contained intranuclear inclusions. by immunohistochemistry, aujeszky disease virus (suid herpesvirus 1) antigen was ... | 2012 | 22308232 |