Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| detection of human cytomegalovirus, human herpesvirus type 6 and human herpesvirus type 7 in urine specimens by multiplex pcr. | to develop a sensitive multiplex pcr to detect hcmv, hhv6 and hhv7, to test this pcr on urine specimens sent to the virus diagnostic laboratory and on stored urine samples from hiv-positive patients and their hiv-negative partners and to compare the sensitivity of the multiplex pcr with the diagnostic laboratory's routine service for the detection of hcmv. | 2003 | 12850164 |
| benzothiadiazine dioxide human cytomegalovirus inhibitors: synthesis and antiviral evaluation of main heterocycle modified derivatives. | the benzothiadiazine dioxide derivatives are potent non-nucleoside human cytomegalovirus (hcmv) inhibitors. as part of our comprehensive structure-activity relationship (sar) study of these compounds, we have now proposed structural modifications on the heterocyclic moiety both on the number and the nature of the fused heterocycle and on the kind of heteroatoms present on it. synthesis of these new compounds (benzyl derivatives of thiadiazines, thienothiadiazines, benzothienothiadiazines and qui ... | 2003 | 12856922 |
| high prevalence of human cytomegalovirus in prostatic intraepithelial neoplasia and prostatic carcinoma. | recent epidemiological data indicate that a history of increased exposure to sexually transmitted diseases is associated with an increased risk of prostate cancer. human cytomegalovirus (hcmv) is a member of the herpesvirus family, is sexually transmitted in adults and can persistently infect prostatic epithelium in non-immunocompromised hosts. based on increased awareness of the oncogenic potential of this virus, we decided to reexplore the issue of whether hcmv might be involved in prostate ca ... | 2003 | 12913758 |
| local character of readthrough activation in adenovirus type 5 early region 1 transcription control. | wild-type early activity of the adenovirus 5 e1b gene promoter requires readthrough transcription originating from the adjacent upstream e1a gene. this unusual mode of viral transcription activation was identified by genetic manipulation of the mouse beta(maj)-globin gene transcription termination sequence (ggt) inserted into the e1a gene. to facilitate further study of the mechanism of readthrough activation, the activities of ggt and a composite termination sequence ct were tested in recombina ... | 2003 | 12915542 |
| human cytomegalovirus, human herpesvirus-6 and human herpesvirus-7 in neutropenic patients with fever of unknown origin. | to investigate the appearance of cytomegalovirus (cmv) dna, human herpesvirus-6 (hhv-6) dna and human herpesvirus-7 (hhv-7) dna in plasma as a sign of reactivation and possible causes of fever of unknown origin (fuo) during neutropenia. | 2003 | 12925104 |
| cytomegalovirus and epstein-barr virus are associated with symptomatic periapical pathosis. | recent studies have identified human cytomegalovirus (hcmv) and epstein-barr virus (ebv) in symptomatic periapical lesions. little information exists on hcmv and ebv in asymptomatic periapical lesions. | 2003 | 12930527 |
| transactivation of a viral target gene by herpes simplex virus icp27 is posttranscriptional and does not require the endogenous promoter or polyadenylation site. | icp27 is an essential herpes simplex virus type 1 (hsv-1) immediate-early protein that stimulates viral mrna expression from many viral delayed-early and late genes during infection. one hsv-1 late gene which is highly dependent on icp27 during infection is that encoding the glycoprotein c (gc). here we report that the gc gene is specifically transactivated by icp27 in transfected vero cells. using various gc plasmid constructs, we show that icp27's stimulatory effects are independent of the gc ... | 2003 | 12941897 |
| engineering of rnase p ribozyme for gene-targeting applications. | ribonuclease p (rnase p) is a ubiquitous ribonucleoprotein complex responsible for the biosynthesis of trna. this enzyme from escherichia coli contains a catalytic rna subunit (m1 ribozyme) and a protein subunit (c5 cofactor). m1 ribozyme cleaves an rna helix that resembles the acceptor stem and t-stem structure of its natural trna substrate. when covalently linked with a guide sequence, m1 rna can be engineered into a sequence-specific endonuclease, m1gs ribozyme, which can cleave any target rn ... | 2003 | 12957377 |
| early identification of human cytomegalovirus strains by the shell vial assay is prevented by a novel amino acid substitution in ul123 ie1 gene product. | 2003 | 12958307 | |
| mapping ganciclovir resistance in the human herpesvirus-6 u69 protein kinase. | human herpesvirus-6 (hhv-6) is a growing concern in immunocompromised individuals, such as in the transplant setting. alone, or in concert with human cytomegalovirus (hcmv), infections with hhv-6 are often severe enough to require antiviral therapy, generally in the form of ganciclovir (gcv). gcv resistance in hcmv is well documented, both clinically and in the laboratory, and has been shown to result from mutations in the ul97 protein kinase and/or ul54 dna polymerase. gcv resistance in hhv-6 h ... | 2003 | 12966551 |
| dislocation of a type i membrane protein requires interactions between membrane-spanning segments within the lipid bilayer. | the human cytomegalovirus gene product us11 causes rapid degradation of class i major histocompatibility complex (mhci) heavy chains by inducing their dislocation from the endoplasmic reticulum (er) and subsequent degradation by the proteasome. this set of reactions resembles the endogenous cellular quality control pathway that removes misfolded or unassembled proteins from the er. we show that the transmembrane domain (tmd) of us11 is essential for mhci heavy chain dislocation, but dispensable ... | 2003 | 12972557 |
| the human cytomegalovirus ul97 protein kinase, an antiviral drug target, is required at the stage of nuclear egress. | human cytomegalovirus encodes an unusual protein kinase, ul97, that activates the established antiviral drug ganciclovir and is specifically inhibited by a new antiviral drug, maribavir. we used maribavir and a ul97 null mutant, which is severely deficient in viral replication, to determine what stage of virus infection critically requires ul97. compared with wild-type virus, there was little or no decrease in immediate-early gene expression, viral dna synthesis, late gene expression, or packagi ... | 2003 | 12502806 |
| tn7-mediated introduction of dna sequences into bacmid-cloned cytomegalovirus genomes for rapid recombinant virus construction. | our basic understanding of how viruses infect, replicate, and cause disease has been largely derived from genetic approaches in which viral sequences have been mutated and the consequences of those mutations determined. in the herpesvirus field, deletions or insertions that preclude expression of specific viral proteins or remove critical cis elements have been invaluable in identifying their overall functions. we are now ready to move to a new level of detail-mapping functional domains within v ... | 2003 | 12505633 |
| mutations conferring foscarnet resistance in a cohort of patients with acquired immunodeficiency syndrome and cytomegalovirus retinitis. | the clinical significance of cytomegalovirus (cmv) foscarnet resistance was studied in patients with acquired immunodeficiency syndrome and cmv retinitis. sequencing of the cmv pol gene was performed in 30 isolates. phenotypic resistance was characterized by the dna hybridization assay (dha) in 30 isolates and by plaque-reduction assay (pra) in 18 isolates. nine isolates had foscarnet resistance mutations, including v787l and e756q that were confirmed by marker transfer experiments. seven of 9 i ... | 2003 | 12599051 |
| inter- and intragenic variations complicate the molecular epidemiology of human cytomegalovirus. | human cytomegalovirus isolates were analyzed, both by restriction fragment-length polymorphism typing and by sequencing for intra- and intergenic variability at 9 sites on the genome, to determine whether genetic variation influenced disease outcome and whether linkage among genes could be identified. variation at the ul55 (glycoprotein b [gb]), ul74 (go), ul75 (gh), ul115 (gl), us9, and us28 gene open-reading frames was studied in relationship to outcome of cytomegalovirus disease. major findin ... | 2003 | 12599055 |
| human cytomegalovirus protein pp65 mediates accumulation of hla-dr in lysosomes and destruction of the hla-dr alpha-chain. | human cytomegalovirus (hcmv) has developed multiple strategies to escape immune recognition. here, we demonstrate that hcmv down-regulates hla-dr expression in infected interferon gamma (ifn-gamma)-stimulated fibroblasts at 1 day after infection. decreased hla-dr expression was not observed on cells infected with an hcmv strain lacking the pp65 gene (rvad65), but was observed on cells transfected with the pp65 gene. hla-dr expression accumulated in vacuoles near the nucleus in hcmv-infected, but ... | 2003 | 12609847 |
| the human cytomegalovirus ul82 gene product (pp71) accelerates progression through the g1 phase of the cell cycle. | as viruses are reliant upon their host cell to serve as proper environments for their replication, many have evolved mechanisms to alter intracellular conditions to suit their own needs. for example, human cytomegalovirus induces quiescent cells to enter the cell cycle and then arrests them in late g(1), before they enter the s phase, a cell cycle compartment that is presumably favorable for viral replication. here we show that the protein product of the human cytomegalovirus ul82 gene, pp71, ca ... | 2003 | 12610120 |
| proteasome-dependent, ubiquitin-independent degradation of the rb family of tumor suppressors by the human cytomegalovirus pp71 protein. | most of the substrates degraded by the proteasome are marked with polyubiquitin chains. however, there are a limited number of examples of nonubiquitinated proteins that are degraded by the proteasome. here, we describe the degradation of the retinoblastoma family of tumor suppressor proteins by the proteasome in the absence of polyubiquitination. the retinoblastoma protein (p105), p107, and p130 are each targeted for degradation by the pp71 protein, which is encoded by the ul82 gene of human cy ... | 2003 | 12626766 |
| use of transgenic hla a*0201/kb and hhd ii mice to evaluate frequency of cytomegalovirus ie1-derived peptide usage in eliciting human cd8 cytokine response. | unlike the pp65 protein of human cytomegalovirus (cmv), which has an immunodominant peptide, pp65(495-503), recognized by human cd8(+) cells in the context of hla a*0201, the fine peptide specificity for cmv ie1 has shown no such immunodominance. with the use of transgenic hla a*0201/kb and hhd ii mice, a selected pool of ie1 peptides, including ie1(p256-264), ie1(p297-304), and ie1(p316-324), were shown to stimulate cytolytic t-lymphocyte lysis in the context of hla a*0201. based on an intracel ... | 2003 | 12634406 |
| relative dominance of hla-b*07 restricted cd8+ t-lymphocyte immune responses to human cytomegalovirus pp65 in persons sharing hla-a*02 and hla-b*07 alleles. | cd8(+) t-cell responses to three human cytomegalovirus (cmv) pp65 epitopes were studied in panels of healthy seropositive hla-a*02/hla-b*07 individuals, and hla-a*02 donors mismatched for hla-b*07. the majority of the latter had significant responses to a hla-a*02-restricted epitope within the cmv pp65 antigen. by contrast, the strongest responses to cmv in the first group were to hla-b*07-restricted epitopes. similar immunodominance of hla-b*07 over hla-a*02 was found in two immunocompromised h ... | 2003 | 12651070 |
| cytomegalovirus and epstein-barr virus dna transcription in endodontic symptomatic lesions. | productive herpesviridae infections are implicated in the etio-pathogenesis of aggressive periodontitis. however, virtually nothing is known about a possible role of herpesviruses in pulpal and periapical pathosis. this study employed a cdna analysis to determine transcription of human cytomegalovirus (hcmv), epstein-barr virus (ebv) and herpes simplex virus (hsv) in 14 recalcitrant periapical lesions and in 2 periapical healthy control sites. | 2003 | 12654100 |
| synthesis and antiviral activity of 1,3-disubstituted uracils against hiv-1 and hcmv. | the development of new non-nucleoside reverse transcriptase inhibitors (nnrtis) is an efficient strategy for finding new therapeutic agents against human immunodeficiency virus (hiv). a large number of 6-substituted uracil derivatives have been prepared in order to explore new nnrtis. however, there are few approaches to anti-hiv agents from 1,3-disubstituted uracil derivatives. therefore, we tried to prepare several 1,3-disubstituted uracils, which were easily obtainable from uracil by preparat ... | 2003 | 14694990 |
| human cytomegalovirus (hcmv) ie1 plays role in resistance to apoptosis with etoposide in cancer cell line by cdk2 accumulation. | human cytomegalovirus (hcmv) has many strategies to survive the attack of the host. hcmv infection of host cells induces cellular activation and disturbance of the cell cycle. it is possible that hcmv modulates the behavior of certain cancer cells that are susceptible to hcmv infection. this study was performed to identify the possible mechanism of resistance to apoptotic stimuli in some cancer cell lines by hcmv infection. hcmv-infected cancer cells showed resistance to apoptosis induced by the ... | 2003 | 14695446 |
| synthesis and antiviral activity of 2-substituted analogs of triciribine. | triciribine (tcn) and triciribine monophosphate (tcn-p) have antiviral and antineoplastic activity at low or submicromolar concentrations. in an effort to improve and better understand this activity, we have conducted a structure-activity relationship study to explore the effect of substitutions at the 2-position of triciribine. 2-methyl- (2-me-tcn), 2-ethyl- (2-et-tcn), 2-phenyl- (2-ph-tcn), 2-chloro- (2-cl-tcn), and 2-aminotriciribine (2-nh2-tcn) were designed and synthesized to determine the ... | 2003 | 14714765 |
| avidity maturation following immunization with two human cytomegalovirus (cmv) vaccines: a live attenuated vaccine (towne) and a recombinant glycoprotein vaccine (gb/mf59). | two human cytomegalovirus (cmv) vaccines have been previously evaluated for their immunogenicity: a recombinant gb/mf59 vaccine and an attenuated strain of cmv (towne). in healthy adults, we measured the antibody avidity maturation indices that occurred after vaccination with each. for towne, administered as a single dose, the rise in igg antibody avidity to cmv glycoprotein gb occurred slowly and continued for 24 months post-immunization. for gb/mf59, administered as two priming doses and a boo ... | 2003 | 14733736 |
| human cytomegalovirus inhibits differentiation of monocytes into dendritic cells with the consequence of depressed immunological functions. | human cytomegalovirus (hcmv) infections in immunocompromised patients are associated with impaired immunological functions. blood monocytes, which can differentiate into dendritic cells upon cytokine stimulation, play a central role in adequate immune reactivity and are believed to carry latent hcmv. we demonstrate here that hcmv infection of monocytes results in a block in the cytokine-induced differentiation of monocytes into functionally active cd1a-positive dendritic cells, which exhibited s ... | 2003 | 14512544 |
| role of pacs-1 in trafficking of human cytomegalovirus glycoprotein b and virus production. | the final envelopment of herpesviruses during assembly of new virions is thought to occur by the budding of core viral particles into a late secretory pathway organelle, the trans-golgi network (tgn), or an associated endosomal compartment. several herpesvirus envelope glycoproteins have been previously shown to localize to the tgn when expressed independently from other viral proteins. in at least some cases this tgn localization has been shown to be dependent on clusters of acidic residues wit ... | 2003 | 14512558 |
| co-detection and discrimination of six human herpesviruses by multiplex pcr-elaha. | herpesviruses are a significant cause of human morbidity. traditional approaches to the identification of these viruses require infectious or at least antigenic virus. multiplex pcr (mpcr) is capable of simultaneously amplifying a range of targets from a single preparation of nucleic acids and when combined with a suitable detection assay, it is capable of discriminating each of the amplicons. | 2003 | 14522068 |
| frequency of human cytomegalovirus-specific t cells during pregnancy determined by intracellular cytokine staining. | the characteristics of cytomegalovirus (cmv)-specific t-cell immunity was investigated in pregnant women with primary, latent, or reactivated cmv infection, and in a comparative group of non-pregnant women. forty-six pregnant and 8 non-pregnant women were examined based on the presence of serum antibody activity against cmv and viral excretion in urine. the frequency of cmv-specific cd4(+) t cells in peripheral blood lymphocytes was determined by staining for intracellular cytokines, interferon ... | 2003 | 14556265 |
| resistance of human cytomegalovirus to d- and l-ribosyl benzimidazoles as a tool to identify potential targets for antiviral drugs. | 2003 | 14565505 | |
| synthesis and antiviral activities of 3-aralkylthiomethylimidazo[1,2-b]pyridazine derivatives. | the synthesis of novel substituted 3-aralkylthiomethylimidazo[1,2-b]pyridazines is reported. all of the synthesized compounds are devoid of antiviral activity against the replication of human immunodeficiency virus. however, compounds 6-chloro-8-methyl-3-phenethylthioimidazo[1,2-b]pyridazine and 6-chloro-2-methyl-3-phenethylthioimidazo[1,2-b]pyridazine are potent inhibitors of the replication of human cytomegalovirus in vitro, while compounds 6-chloro-2-methyl-3-benzylthiomethylimidazo[1,2-b]pyr ... | 2003 | 14582846 |
| design and synthesis of acyclic nucleoside analogs with chlorinated imidazo[1,2-a]pyridine bases. | a series of acyclic c-nucleoside analogs of 2,6-dichloro- and 2,6,7-trichloroimidazo[1,2-a]pyridine were synthesized and tested for antiviral activity. the appropriate hydroxymethyl-substituted heterocycles were treated successively with thionyl chloride, an appropriate nucleophile, then diisopropylethylamine to obtain the desired acyclic nucleoside analogs. these compounds were evaluated for activity against human cytomegalovirus and herpes simplex virus, type 1. two of the dichloro analogs, bu ... | 2003 | 14609230 |
| plasmid dna encoding antigens of infectious bursal disease viruses induce protective immune responses in chickens: factors influencing efficacy. | the complete polyprotein (vp2/4/3) and vp2 genes of two infectious bursal disease viruses (ibdvs) (one attenuated strain jd1 and one virulent strain zj2000) were amplified by long and accurate polymerase chain reaction (la-pcr), cloned, sequenced and inserted into plasmids pci and pcdna3 under the control of human cytomegalovirus (hcmv) immediate early enhancer and promoter. a series of dna vaccine preparations were made using liposome as the adjuvant to examine their immunogenicity. although vp ... | 2003 | 14609631 |
| interactions between human cytomegalovirus ie1-72 and cellular p107: functional domains and mechanisms of up-regulation of cyclin e/cdk2 kinase activity. | previous work has demonstrated that the human cytomegalovirus ie1-72 protein is able to bind to the n terminus of p107, and ie1-72 alone is sufficient for alleviation of p107-mediated cell growth suppression. however, the mechanism of this alleviation is unclear. here, we show that ie1-72 can alleviate p107 inhibition of cyclin e/cdk2 kinase activity. we cotransfected various ie1-72 and p107 constructs into c33a cells and demonstrated that ie1-72 could activate the kinase activity of cyclin e/cd ... | 2003 | 14610188 |
| functional profiling of a human cytomegalovirus genome. | human cytomegalovirus (hcmv), a ubiquitous herpesvirus, causes a lifelong subclinical infection in healthy adults but leads to significant morbidity and mortality in neonates and immunocompromised individuals. its ability to grow in different cell types is responsible for hcmv-associated diseases, including mental retardation and retinitis, and vascular disorders. to globally assess viral gene function for replication in cells, we determined the genomic sequence of a bacterial artificial chromos ... | 2003 | 14623981 |
| human cytomegalovirus proteins pp65 and iep72 are targeted to distinct compartments in nuclei and nuclear matrices of infected human embryo fibroblasts. | the cellular distribution of the human cytomegalovirus (hcmv)-specific ul83 phosphoprotein (pp65) and ul123 immediate-early protein (iep72) in lytically infected human embryo fibroblasts was studied by means of indirect immunofluorescence and confocal microscopy. both proteins were found to have a nuclear localization, but they were concentrated in different compartments within the nuclei. the pp65 was located predominantly in the nucleoli; this was already evident with the parental viral protei ... | 2003 | 14624464 |
| in vitro model for the study of the dissociation of increasing antigenemia and decreasing dnaemia and viremia during treatment of human cytomegalovirus infection with ganciclovir in transplant recipients. | the paradox phenomenon (i.e., the dissociation of increasing antigenemia and decreasing dnaemia and viremia) that occurs during treatment of human cytomegalovirus (hcmv) infections with ganciclovir (gcv), in transplant recipients, was investigated by use of an in vitro model for the study of interactions between polymorphonuclear leukocytes and endothelial cells. the paradox phenomenon was reproduced in vitro in the presence of gcv and, to a much lesser extent, in the presence of cidofovir, but ... | 2003 | 14639533 |
| deletion of the non-essential ul0 gene of infectious laryngotracheitis (ilt) virus leads to attenuation in chickens, and ul0 mutants expressing influenza virus haemagglutinin (h7) protect against ilt and fowl plague. | infectious laryngotracheitis virus (iltv), a member of the alphaherpesvirinae, possesses several unique genes. one of them, ul0, encodes an abundantly expressed protein that accumulates in the nuclei of iltv-infected cells. this study demonstrates that this protein is dispensable for in vitro virus replication and that ul0 deletion mutants exhibit only minor growth defects in cultured cells. the ul0 gene locus of iltv was also used for insertion of foreign dna sequences encoding enhanced gfp or ... | 2003 | 14645915 |
| the human cytomegalovirus ul45 gene product is a late, virion-associated protein and influences virus growth at low multiplicities of infection. | human cytomegalovirus (hcmv) encodes a protein related to the large (r1) subunit of ribonucleotide reductase (rr), but does not encode the corresponding small (r2) subunit. the r1 homologue, ul45, lacks many catalytic residues, and its impact on deoxyribonucleotide (dntp) production remains unknown. here, ul45 is shown to accumulate at late stages of infection and to be a virion tegument protein. to study ul45 function in its genome context, ul45 was disrupted by transposon insertion. the ul45-k ... | 2003 | 14645917 |
| [quantitative determination of cmv-dna in saliva of patients with bone marrow and stem cell transplantation using taqman-pcr]. | human cytomegalovirus (hcmv) infection is associated with severe and life-threatening diseases in immunocompromised patients, especially after bone marrow (bm) and stem cell (sc) transplantation. prior to transplantation the potential risk of hcmv disease is therefore determined by hcmv-antibody blood testing of transplant donor (d) and recipient (r). virus carriers are positive for anti-cmv-igg. virus patterns are distinguished as follows: group 1 (d+/r+), group 2 (d-/r+), group 3 (d+/r-), and ... | 2003 | 14648253 |
| one-tube semi-nested pcr-elisa for the detection of human cytomegalovirus dna sequences; comparison with hybridization-based and semi-nested-based pcr-elisa procedures. | amplification of dna targets by polymerase chain reaction (pcr) followed by their colorimetric detection in an enzyme-linked immunosorbent assay (elisa) is increasingly used in both immunological research and clinical practice. several methods for the labeling and detection of amplified dna sequences have been previously described. in this study, we compared the conventional hybridization-based pcr-elisa with a modified semi-nested and one-tube semi-nested pcr-elisa for the detection of human cy ... | 2003 | 14659908 |
| human cytomegalovirus-encoded us2 differentially affects surface expression of mhc class i locus products and targets membrane-bound, but not soluble hla-g1 for degradation. | human cmv (hcmv) can elude ctl as well as nk cells by modulating surface expression of mhc class i molecules. this strategy would be most efficient if the virus would selectively down-regulate viral ag-presenting alleles, while at the same time preserving other alleles to act as inhibitors of nk cell activation. we focused on the hcmv unique short (us) region encoded protein us2, which binds to newly synthesized mhc class i h chains and supports their dislocation to the cytosol for subsequent de ... | 2003 | 14662880 |
| effective suppression of class i major histocompatibility complex expression by the us11 or icp47 genes can be limited by cell type or interferon-gamma exposure. | an impediment encountered in many viral-based gene therapy clinical trials has been the rapid destruction of the transgene by the host's immune response. the processing and presentation of antigens through the class i major histocompatibility complex (mhc) pathway is the initial specific response to viral infection. disruption of the class i mhc pathway by herpes simplex virus (hsv) or the human cytomegalovirus (hcmv) results in a decrease of the cd8(+) cytotoxic t lymphocyte (ctl) response and ... | 2003 | 14670127 |
| the occurrence of symptomatic cmv infections in heart transplant recipients. | human cytomegalovirus is widely spread in the human population and at present it is the most important clinical agent of opportunistic infections in immunocompromised patients. bone marrow transplant recipients, solid organ transplant recipients and aids patients are at risk of the development of a severe systemic disease. prompt detection of active infection is essential for early initiation of antiviral therapy. | 2003 | 15055732 |
| [effect of human cytomegalovirus on hematopoietic system]. | to investigate the mechanism and the suppression effect of human cytomegalovirus (hcmv) on hematopoietic system. | 2003 | 14751047 |
| proteasome inhibitors: a novel tool to suppress human cytomegalovirus replication and virus-induced immune modulation. | recently, we like others, demonstrated that systemic inflammation is the most important mechanism involved in (re)activation of human cytomegalovirus (hcmv) in both immunocompetent patients. by in vitro studies the eukaryotic transcription factor nf-kappab could be identified as the key mediator of tnf-alpha- and ie1-dependent stimulation of the hcmv ie1/2 enhancer/promoter activity, which is crucial for initiation of viral gene expression during reactivation from latency as well as productive i ... | 2003 | 14760889 |
| [preliminary studies on pathogenic factors of human cytomegalovirus infection]. | to investigate the pathogenic factors of human cytomegalovirus (hcmv) infections. | 2003 | 15340555 |
| detection of human cytomegalovirus dna in 986 women studied for human papillomavirus-associated cervical neoplasia. | the aim of this study was to assess the association of human cytomegalovirus (cmv) infection with cervical histologic findings and possible interaction with human papillomavirus (hpv) infection. | 2003 | 17051067 |
| emergence and compartmentalization of fatal multi-drug-resistant cytomegalovirus infection in a patient with autosomal-recessive severe combined immune deficiency. | the authors describe a patient with autosomal-recessive severe combined immunodeficiency (scid) with severe, multiorgan cytomegalovirus (cmv) disease. in the face of appropriate therapy, the patient developed a 100-fold gradient in viral load across the blood-brain barrier. disseminated disease, including pneumonitis, contributed to a fatal outcome. serial genotypic analyses revealed multiple ul97 and ul54 (dna polymerase) mutations that conferred phenotypic resistance to all currently licensed ... | 2004 | 15342989 |
| immunoglobulin gm genes and igg antibodies to cytomegalovirus in patients with systemic sclerosis. | to determine the role of gm and km genes--genetic markers of immunoglobulin gamma and kappa chains, respectively--in humoral immunity to human cytomegalovirus (hcmv) in patients with systemic sclerosis (ssc; scleroderma). | 2004 | 15344596 |
| human cytomegalovirus trs1 protein is required for efficient assembly of dna-containing capsids. | the human cytomegalovirus tegument protein, ptrs1, appears to function at several discrete stages of the virus replication cycle. we previously demonstrated that ptrs1 acts during the late phase of infection to facilitate the production of infectious virions. we now have more precisely identified the late ptrs1 function by further study of a mutant virus lacking the trs1 region, adsubtrs1. we observed a significant reduction in the production of capsids, especially dna-containing c-capsids, in m ... | 2004 | 15367587 |
| coupling generation of cytomegalovirus deletion mutants and amplification of viral bac clones. | human cytomegalovirus (hcmv) genome manipulation has always been difficult. recently, the introduction of full-length hcmv dna into escherichia coli as an artificial bacterial chromosome (bac) clone has allowed reliable targeted mutagenesis. here, we show the next generation of improvement in designing recombinant hcmv, which will also be applicable to other viral bac clones. an inducible origin of replication linked with an antibiotic resistance marker was used as a cassette for targeted replac ... | 2004 | 15381350 |
| reduced expression of gamma interferon in serum and marked lymphoid depletion induced by porphyromonas gingivalis increase murine morbidity and mortality due to cytomegalovirus infection. | porphyromonas gingivalis, a gram-negative anaerobe, is a major etiological agent of severe forms of periodontal disease. although periodontal disease is considered a localized disease, accumulating evidence indicates that it may lead to a predisposition to a decline in immunocompetence. human cytomegalovirus (cmv) commonly infects all human populations without producing significant clinical symptoms. immunocompromised patients usually develop a primary or reactivated cmv infection, which is asso ... | 2004 | 15385479 |
| human cytomegalovirus infection induces rapamycin-insensitive phosphorylation of downstream effectors of mtor kinase. | signaling mediated by the cellular kinase mammalian target of rapamycin (mtor) activates cap-dependent translation under normal (nonstressed) conditions. however, translation is inhibited by cellular stress responses or rapamycin treatment, which inhibit mtor kinase activity. we show that during human cytomegalovirus (hcmv) infection, viral protein synthesis and virus production proceed relatively normally when mtor kinase activity is inhibited due to hypoxic stress or rapamycin treatment. using ... | 2004 | 15452223 |
| cyclin-dependent kinase activity is required at early times for accurate processing and accumulation of the human cytomegalovirus ul122-123 and ul37 immediate-early transcripts and at later times for virus production. | human cytomegalovirus (hcmv) infection leads to dysregulation of multiple cell cycle-regulatory proteins. in this study, we examined the effects of inhibition of cyclin-dependent kinase (cdk) activity on viral replication. with the drug roscovitine, a specific inhibitor of cyclin-dependent kinases 1, 2, 5, 7, and 9, we have shown that during the first 6 h of infection, cyclin-dependent kinase-dependent events occurred that included the regulated processing and accumulation of the immediate-early ... | 2004 | 15452241 |
| reduction of adenovirus e1a mrna by rnai results in enhanced recombinant protein expression in transiently transfected hek293 cells. | human embryonic kidney 293 (hek293) cells, a widely used host for large-scale transient expression of recombinant proteins, are transformed with the adenovirus e1a and e1b genes. because the e1a proteins function as transcriptional activators or repressors, they may have a positive or negative effect on transient transgene expression in this cell line. suspension cultures of hek293 ebna (hek293e) cells were co-transfected with a reporter plasmid expressing the gfp gene and a plasmid expressing a ... | 2004 | 15474305 |
| the putative zinc finger of the human cytomegalovirus ie2 86-kilodalton protein is dispensable for dna binding and autorepression, thereby demarcating a concise core domain in the c terminus of the protein. | the ie2 86-kda gene product is an essential regulatory protein of human cytomegalovirus (hcmv) with several functions, including transactivation, negative autoregulation, and cell cycle regulation. in order to understand the physiological significance of each of the ie2 functions, discriminating mutants of ie2 are required that can be tested in a viral background. however, no such mutants of ie2 are available, possibly reflecting structural peculiarities of the large and ill-defined c-terminal d ... | 2004 | 15479827 |
| cytomegalovirus following stem cell transplantation: from pharmacologic to immunologic therapy. | human cytomegalovirus is a large dna virus that is well-equipped to evade both innate and adaptive host immune responses and to establish lifelong latency. it is a major opportunistic pathogen in immunocompromised hosts. following allogeneic transplantation, immune responses are often inadequate to inhibit viral reactivation, resulting in progressive tissue damage, manifesting as overt human cytomegalovirus disease that usually presents as pneumonitis, colitis or hepatitis. currently available a ... | 2004 | 15482220 |
| igg antibodies to human cytomegalovirus late protein ul94 in patients with systemic sclerosis. | human cytomegalovirus (hcmv) has been proposed as an amplifying agent for at least some of the spectrum of systemic sclerosis (ssc; scleroderma). in support of this hypothesis, antibodies to the hcmv late protein ul94 have been detected in the majority of ssc patients in a study involving caucasian subjects from italy. the aim of this investigation was to determine whether elevated levels of anti-ul94 antibodies are present in african american and caucasian ssc patients from the u.s. we further ... | 2004 | 15497459 |
| novel chemical class of pul97 protein kinase-specific inhibitors with strong anticytomegaloviral activity. | human cytomegalovirus (hcmv) is a major human pathogen frequently associated with life-threatening disease in immunosuppressed patients and newborns. the hcmv ul97-encoded protein kinase (pul97) represents an important determinant of viral replication. recent studies demonstrated that pul97-specific kinase inhibitors are powerful tools for the control of hcmv replication. we present evidence that three related quinazoline compounds are potent inhibitors of the pul97 kinase activity and block in ... | 2004 | 15504835 |
| synthesis and antiviral evaluation of polyhalogenated imidazole nucleosides: dimensional analogues of 2,5,6-trichloro-1-(beta-d-ribofuranosyl)benzimidazole. | a series of polyhalogenated imidazole nucleosides were designed and synthesized as ring-contracted analogues of 2,5,6-trichloro-1-(beta-d-ribofuranosyl)benzimidazole (tcrb) and its 2-bromo analogue (bdcrb) in an effort to explore the spatial limitation of the active pocket(s) in the target protein(s). 2,4,5-trichloro-, 2-bromo-4,5-dichloro-, and 2,4,5-tribromoimidazole nucleosides were prepared by a condensation of the preformed heterocycles with the appropriate sugar precursors. the ribofuranos ... | 2004 | 15509173 |
| synthesis and antiviral activity of 3-formyl- and 3-cyano-2,5,6-trichloroindole nucleoside derivatives. | a series of trichlorinated indole nucleosides has been synthesized and tested for activity against human cytomegalovirus (hcmv) and herpes simplex virus type-1 (hsv-1) and for cytotoxicity. the previously reported 3-formyl-2,5,6-trichloro-1-(beta-d-ribofuranosyl)indole (ftcri) and its 3-cyano homologue (ctcri) were chemically modified at the 3-position. the formation of hydrazones and oximes of ftcri was accomplished by a dehydrative addition of the appropriate hydrazine or hydroxylamine derivat ... | 2004 | 15509175 |
| human cytomegalovirus encodes a highly specific rantes decoy receptor. | the human cytomegalovirus pul21.5 protein is a small, secreted glycoprotein whose mrna is packaged into virions. we demonstrate that pul21.5 protein is a soluble cc chemokine receptor that functions as a decoy to modulate the host immune response to infection. in contrast to other viral chemokine-binding proteins, which interact promiscuously with multiple chemokines, pul21.5 selectively binds rantes (regulated upon activation, normal t cell expressed and secreted) with high affinity. by binding ... | 2004 | 15536129 |
| lack of induction of autoantibody responses following immunization with cytomegalovirus (cmv) glycoprotein b (gb) in healthy cmv-seronegative subjects. | possible correlations have been proposed between autoimmune diseases, such as systemic lupus erythematosus (sle), and infection with human cytomegalovirus (cmv). the recent observation that an adenovirus expressing the immunodominant envelope glycoprotein of cmv, glycoprotein b (gb), may be capable of inducing autoantibodies in certain mouse strains has prompted interest in exploring potential relationships between gb immunization and autoimmune disease. we examined whether a recombinant cmv gb ... | 2004 | 15542191 |
| human cytomegalovirus-specific cd4+-t-cell cytokine response induces fractalkine in endothelial cells. | cytomegalovirus (cmv) infection has been linked to inflammation-related disease processes in the human host, including vascular diseases and chronic transplant rejection. the mechanisms through which cmv affects the pathogenesis of these diseases are for the most part unknown. to study the contributing role of the host immune response to cmv in these chronic inflammatory processes, we examined endothelial cell interactions with peripheral blood mononuclear cells (pbmc). endothelial cultures were ... | 2004 | 15542669 |
| expression and characterization of a soluble form of tomato spotted wilt virus glycoprotein gn. | tomato spotted wilt virus (tswv), a member of the tospovirus genus within the bunyaviridae, is an economically important plant pathogen with a worldwide distribution. tswv is transmitted to plants via thrips (thysanoptera: thripidae), which transmit the virus in a persistent propagative manner. the envelope glycoproteins, g(n) and g(c), are critical for the infection of thrips, but they are not required for the initial infection of plants. thus, it is assumed that the envelope glycoproteins play ... | 2004 | 15542672 |
| interleukin-2 immunotherapy and aids-related cytomegalovirus retinitis. | cytokines are small proteins produced by t lymphocytes that mediate immune responses. those produced by the cd4+ th1 subset induce cell-mediated immunity, whereas those produced by the cd4+ th2 subset are more efficient at stimulating immunoglobulin production. the goal of cytokine immunotherapy is prevention or reduction of disease progression through stimulation of cell-mediated immunity (i.e., immune reconstitution) by administration of an exogenous th1 cytokine such as interleukin-2 (il-2). ... | 2004 | 15544454 |
| 5-(1-substituted) alkyl pyrimidine nucleosides as antiviral (herpes) agents. | the treatment of viral diseases remains one of the major challenges to modern medicine. during the past two decades there has been increased recognition of the consequences of serious viral illnesses that are not controlled by vaccination. these illnesses include human immunodeficiency virus, human herpes viruses, and viruses that cause hepatitis. there are now eight pathogens recognized in the herpes virus family that cause infections in humans. infections by the herpes viruses are opportunisti ... | 2004 | 15544474 |
| permissive human cytomegalovirus infection of a first trimester extravillous cytotrophoblast cell line. | human cytomegalovirus (hcmv) is the leading cause of congenital viral infection in the united states and europe. despite the significant morbidity associated with prenatal hcmv infection, little is known about how the virus infects the fetus during pregnancy. to date, primary human cytotrophoblasts (ctbs) have been utilized to study placental hcmv infection and replication; however, the minimal mitotic potential of these cells restricts experimentation to a few days, which may be problematic for ... | 2004 | 15548334 |
| down-regulation of cxcr4 expression on human cd8+ t cells during peripheral differentiation. | multi-color flow cytometric analysis on human cd8(+) t cell subsets revealed that cxcr4 is predominantly expressed on cd8(+) t cells with the naive cd27(+)cd28(+)cd45ra(+) phenotype, and is down-regulated during differentiation into those with an effector phenotype. the down-regulation of cxcr4 expression during peripheral differentiation was supported by the fact that the expression of cxcr4 on cd8(+) t cells was negatively correlated with that of perforin. the analysis of ccr5, ccr7, and cxcr4 ... | 2004 | 15549771 |
| alteration of cellular rna splicing and polyadenylation machineries during productive human cytomegalovirus infection. | alternative processing of human cytomegalovirus (hcmv) ul37 pre-mrna predominantly produces the unspliced ul37 exon 1 (ul37x1) rna and multiple, lower abundance, alternatively spliced ul37 rnas. the relative abundance of ul37x1 unspliced rna is surprising because it requires the favoured use of a polyadenylation signal within ul37 intron 1, just upstream of the ul37 exon 2 (ul37x2) acceptor. here, it was shown that a downstream element (dse) in ul37x2 strongly enhanced processing at the ul37x1 p ... | 2004 | 15557227 |
| a single tetracycline-regulated vector devised for controlled insulin gene expression. | to construct a single plasmid vector mediating doxycycline-inducible recombined human insulin gene expression in myotube cell line. | 2004 | 15669184 |
| human cytomegalovirus proteins pp65 and immediate early protein 1 are common targets for cd8+ t cell responses in children with congenital or postnatal human cytomegalovirus infection. | recombinant modified vaccinia ankara- and peptide-based ifn-gamma elispot assays were used to detect and measure human cmv (hcmv)-specific cd8(+) t cell responses to the pp65 (ul83) and immediate early protein 1 (ie1; ul123) gene products in 16 hcmv-infected infants and children. age at study ranged from birth to 2 years. hcmv-specific cd8(+) t cells were detected in 14 (88%) of 16 children at frequencies ranging from 60 to >2000 spots/million pbmc. responses were detected as early as 1 day of a ... | 2004 | 14764694 |
| kinetics of cytomegalovirus load decrease in solid-organ transplant recipients after preemptive therapy with valganciclovir. | the availability of valganciclovir (vgcv) has significantly simplified the treatment of human cytomegalovirus (hcmv) infection after solid-organ transplantation. we show that there was no difference in the kinetics of the decrease in hcmv load after preemptive therapy with vgcv in 22 solid-organ transplant recipients (t1/2=2.16 days), compared with that in 23 patients treated with intravenous ganciclovir (gcv) (t(1/2) = 1.73 days; p=.63). preemptive therapy with vgcv provides control of hcmv rep ... | 2004 | 15593008 |
| the correlation between prognosis of hcmv infection and zinc in mature women. | the aim of the study was to investigate the correlation between prognosis of human cytomegalovirus (hcmv) infection and zinc in mature women, in order to explore the effect of serum zinc on hcmv infection. 900 mature woman were examined for zinc level and serum hcmv specific antibodies (igg and igm). 63 hcmv-igm positive cases were divided into 3 subgroups according to their serum zinc level, and hcmv-igm was observed for conversion after treatment with a chinese traditional medicine (jinyebadu) ... | 2004 | 15791862 |
| qualitative detection of human cytomegalovirus dna in the plasma of bone marrow transplant recipients: value as a predictor of disease progression. | the aim of this prospective study was to determine whether human cytomegalovirus (hcmv)-dna detected by polymerase chain reaction (pcr) analysis in the plasma of bone marrow transplant (bmt) patients is a predictor of hcmv disease progression. | 2004 | 15859928 |
| in vitro anti-cytomegalovirus activity of kampo (japanese herbal) medicine. | we examined the effect of three types of kampo medicines on human cytomegalovirus (cmv) replication in the human embryonic fibroblast cell line, mrc-5. treatment of cells with at least 0.01 mug/ml of kampo medicines inhibited the cytopathic effects of cmv-infected mrc-5 cells. moreover, kampo medicine decreased the replication of cmv without affecting the inhibition of host cells, with a concomitant decrease in cmv dna levels. however, kampo medicine demonstrated no virocidal effect on cell-free ... | 2004 | 15841262 |
| insights into the structure of human cytomegalovirus large terminase subunit pul56. | terminases are a class of proteins which catalyze the generation of unit-length genomes during dna packaging. these essential proteins are conserved throughout the herpesviruses and many double-stranded dna bacteriophages. we have determined the structure of the large terminase subunit pul56 of human cytomegalovirus, a highly pathogenic virus, to 2.6 nm resolution. image analysis of purified pul56 suggests that the molecule exists as a dimer formed by the association of two ring-like structures ... | 2004 | 15063737 |
| differential mutagen sensitivity of peripheral blood lymphocytes from smokers and nonsmokers: effect of human cytomegalovirus infection. | we used the mutagen sensitivity assay to test the hypothesis that human cytomegalovirus (hcmv) infection modifies the sensitivity of cells to genetic damage from genotoxic agents. chromosome aberration (ca) frequency in peripheral blood lymphocytes (pbls) from 20 smokers who were matched with 20 nonsmokers by age (+/- 5 years), sex, and ethnicity was evaluated following in vitro exposure to bleomycin and/or hcmv infection. bleomycin induced significant (p < 0.05) concentration-dependent increase ... | 2004 | 15065204 |
| discovery of a second form of tripartite complex containing gh-gl of human herpesvirus 6 and observations on cd46. | the human herpesvirus 6 (hhv-6) glycoprotein h (gh)-glycoprotein l (gl) complex associates with glycoprotein q (gq) (y. mori, p. akkapaiboon, x. yang, and k. yamanishi, j. virol. 77:2452-2458, 2003), and the gh-gl-gq complex interacts with human cd46 (y. mori, x. yang, p. akkapaiboon, t. okuno, and k. yamanishi, j. virol. 77:4992-4999, 2003). here, we show that the hhv-6 u47 gene, which is a positional homolog of the human cytomegalovirus glycoprotein o (go) gene, encodes a third component of th ... | 2004 | 15078943 |
| the use of clonal mrna as an antigenic format for the detection of antigen-specific t lymphocytes in ifn-gamma elispot assays. | most assay systems for the quantification of antigen-specific t-cell responses in infectious, malignant and autoimmune disease depend on the peptide antigen format and are therefore restricted to known epitopes and their presenting hla molecules. here we tested in elispot assays the application of in vitro-transcribed clonal mrna as an alternative antigen format covering all potential epitopes of a given antigen. as model antigens, we chose pp65 of human cytomegalovirus (hcmv) and human tyrosina ... | 2004 | 15099761 |
| genetic content of wild-type human cytomegalovirus. | the genetic content of wild-type human cytomegalovirus was investigated by sequencing the 235 645 bp genome of a low passage strain (merlin). substantial regions of the genome (genes rl1-ul11, ul105-ul112 and ul120-ul150) were also sequenced in several other strains, including two that had not been passaged in cell culture. comparative analyses, which employed the published genome sequence of a high passage strain (ad169), indicated that merlin accurately reflects the wild-type complement of 165 ... | 2004 | 15105547 |
| hcmv activates pi(3)k in monocytes and promotes monocyte motility and transendothelial migration in a pi(3)k-dependent manner. | human cytomegalovirus (hcmv) is a leading cause of morbidity and mortality in immunocompromised hosts. in immunocompetent hosts, hcmv is associated with chronic inflammatory diseases including atherosclerosis. monocytes and macrophages are proposed to play key roles in hcmv dissemination to host tissue, and their infection provides a biological link between the lifecycle of hcmv and disease pathology. we hypothesize that viral spread occurs via a mechanism in which infected peripheral blood mono ... | 2004 | 15107461 |
| a novel pattern of pp65-positive cytomegalic endothelial cells circulating in peripheral blood from a renal transplant recipient. | the present study reports a novel pattern of cytomegalic endothelial cells (cec) in peripheral blood from a female renal transplant recipient infected with human cytomegalovirus (hcmv), which has not been reported previously. localization of specific early antigen of hcmv, pp65 antigen, was examined by immunohistochemistry. staining of an endothelial cell marker (cd34) was used to characterize endothelial cells. it is demonstrated that many leukocytes surrounded and adhered to a protein-like mat ... | 2004 | 15147631 |
| induction of enhanced green fluorescent protein expression in response to lesions in the nervous system. | we have generated a mouse strain carrying a transgene driven by a strong and ubiquitous promoter (human cytomegalovirus hcmv/beta-actin) and containing an enhanced green fluorescent protein (egfp) coding sequence upstream of the 3' untranslated region (3'utr) of tissue-type plasminogen activator (t-pa) mrna. the 3'utr of t-pa mrna is known to be involved in the reversible deadenylation and translational repression of transcripts in mouse oocytes. hcmv/beta-actin-egfp-3'utr t-pa transgenic mice e ... | 2004 | 15156581 |
| protective immunity to siv challenge elicited by vaccination of macaques with multigenic dna vaccines producing virus-like particles. | we utilized siv(mne) infection of macaca fascicularis to assess the efficacy of dna vaccination alone, and as a priming agent in combination with subunit protein boosts. all siv(mne) structural and regulatory genes were expressed using the human cytomegalovirus immediate early-1 promoter in plasmids that directed the formation of virus-like particles in vitro. macaques (n = 4) were immunized intradermally and intramuscularly four times over 36 weeks with 3 mg plasmid dna. a second group (n = 4) ... | 2004 | 15157361 |
| ability of the human cytomegalovirus ie1 protein to modulate sumoylation of pml correlates with its functional activities in transcriptional regulation and infectivity in cultured fibroblast cells. | in one of the earliest events in human cytomegalovirus (hcmv)-infected cells, the major immediate-early (ie) protein ie1 initially targets to and then disrupts the nuclear structures known as pml oncogenic domains (pods) or nuclear domain 10. recent studies have suggested that modification of pml by sumo is essential to form pods and that ie1 both binds to pml and may disrupt pods by preventing or removing sumo adducts on pml. in this study, we showed that in contrast to herpes simplex virus typ ... | 2004 | 15163746 |
| clinically-based determination of safe dnaemia cutoff levels for preemptive therapy or human cytomegalovirus infections in solid organ and hematopoietic stem cell transplant recipients. | transplantation centers using human cytomegalovirus (hcmv) antigenemia-based preemptive therapy will need to replace in the near future the antigenemia assay with a more standardized and automatable assay, such as a molecular assay quantifying hcmv dna in blood (dnaemia). thus, in view of replacing antigenemia with clinically safe cutoff values, dnaemia levels corresponding to antigenemia cutoffs guiding hcmv preemptive therapy were determined retrospectively in solid organ and hematopoietic ste ... | 2004 | 15170637 |
| properties of cd4(+) t cells in human cytomegalovirus infection. | the correlates of protective immunity to disease-inducing viruses in man remain to be elucidated. we determined the kinetics and properties of cytomegalovirus (cmv)-specific cd4(+) t cells in healthy individuals and renal transplant recipients during different stages of cmv infection. our data reveal that circulating cmv-specific cd4(+) t cells displayed an effector-memory phenotype, and produced the t helper 1 cytokines interferon-gamma and tumor necrosis factor-alpha. in addition, they lacked ... | 2004 | 15172448 |
| augmentation of virus-specific immunity after hematopoietic stem cell transplantation by adoptive t-cell therapy. | adoptive transfer of virus-specific t cells offers the potential for accelerating reconstitution of antigen-specific immunity after allogeneic hematopoietic stem cell transplantation. however, the logistics of producing virus-specific t cells and the risk of inducing graft-versus-host disease has limited their application. we developed a relatively simple system employing cytomegalovirus lysate-pulsed, monocyte-derived dendritic cells as stimulator cells, requiring only a single blood draw from ... | 2004 | 15172456 |
| human cytomegalovirus-induced host cell enlargement is iron dependent. | a hallmark of human cytomegalovirus (hcmv) infection is the characteristic enlargement of the host cells (i.e., cytomegaly). because iron (fe) is required for cell growth and fe chelators inhibit viral replication, we investigated the effects of hcmv infection on fe homeostasis in mrc-5 fibroblasts. using the metallosensitive fluorophore calcein and the fe chelator salicylaldehyde isonicotinoyl hydrazone (sih), the labile iron pool (lip) in mock-infected cells was determined to be 1.04 +/- 0.05 ... | 2004 | 15175225 |
| phosphorylation of the rna polymerase ii carboxyl-terminal domain in human cytomegalovirus-infected cells and in vitro by the viral ul97 protein kinase. | the carboxyl-terminal domain (ctd) of the largest subunit of rna polymerase ii (rnap ii) ordinarily exists in electrophoretically distinct hypophosphorylated and hyperphosphorylated forms. human cytomegalovirus infection induced forms of this subunit whose electrophoretic mobilities were intermediate without decreases in abundance of the original forms. phosphatase treatment nearly eliminated the intermediate migrating forms. in vitro, the viral protein kinase, ul97, phosphorylated this subunit, ... | 2004 | 15183065 |
| mutations in the human cytomegalovirus ul27 gene that confer resistance to maribavir. | previous drug selection experiments resulted in the isolation of a human cytomegalovirus (cmv) ul97 phosphotransferase mutant resistant to the benzimidazole compound maribavir (1263w94), reflecting the anti-ul97 effect of this drug. three other cmv strains were plaque purified during these experiments. these strains showed lower-grade resistance to maribavir than the ul97 mutant. extensive dna sequence analyses showed no changes from the baseline strain ad169 in ul97, the genes involved in dna r ... | 2004 | 15194788 |
| cytomegalovirus infection in organ-transplant recipients: diagnostic value of pp65 antigen test, qualitative polymerase chain reaction (pcr) and quantitative taqman pcr. | the human cytomegalovirus (cmv) is a major cause of morbidity and mortality in transplant patients. in this study, we compared the diagnostic value of pp65 antigen test, qualitative nested polymerase chain reaction (pcr), and quantitative taqman pcr in predicting the clinical outcome of cmv infection. | 2004 | 15201668 |
| herpesviral-bacterial interrelationships in aggressive periodontitis. | recent findings have begun to provide a basis for a causal link between herpesviruses and aggressive periodontitis. one theory is that herpesviruses cooperate with specific bacteria in the etiopathogenesis of the disease. this study examined whether the presence of herpesviruses [human cytomegalovirus (hcmv), epstein-barr virus (ebv) type 1, herpes simplex virus (hsv) type 1 and 2] is associated with the presence of putative pathogenic bacteria (porphyromonas gingivalis, prevotella intermedia, t ... | 2004 | 15206912 |
| clinical applications of real-time pcr for diagnosis and treatment of human cytomegalovirus infection in children. | there are many methods of detecting human cytomegalovirus (hcmv) infection. so far, the quantitative polymerase chain reaction (pcr) has been very useful not only in aiding in the diagnosis of hcmv but also in determining the severity and predicting hcmv infection. however, it is time-consuming and labor intensive. real-time pcr (rt-pcr) is an exception, for it allows rapid quantification of hcmv dna load. our group used this method for detecting and monitoring hcmv and compared it with the diag ... | 2004 | 15209952 |
| downregulation of the cellular adhesion molecule thy-1 (cd90) by cytomegalovirus infection of human fibroblasts. | the deregulation of cellular adhesion molecules by human cytomegalovirus (hcmv) appears to be correlated with the development of vascular disease. in this study, it was investigated whether the expression of thy-1 (cd90), a member of the immunoglobulin superfamily of adhesion molecules with constitutive expression on fibroblast cells, is modulated following infection with hcmv. it was observed that thy-1 cell surface expression decreased significantly during the course of infection. addition of ... | 2004 | 15218185 |
| detection of rna in purified cytomegalovirus virions. | five viral rna transcripts have recently been detected in purified virions of human cytomegalovirus (hcmv) strain ad169, a well-characterized member of the family herpesviridae [science 288 (2000) 2373]. while the function of these transcripts and/or the proteins they encode remains to be elucidated, it is not known whether these transcripts are unique to strain ad169 or are present in other hcmv strains. the objective of this study was to determine if these rnas are present in other hcmv labora ... | 2004 | 15246650 |
| analysis of splice variants of the immediate-early 1 region of human cytomegalovirus. | the major immediate-early (mie) gene of human cytomegalovirus (hcmv) produces multiple mrnas through differential splicing and polyadenylation. reverse transcriptase pcr was used to characterize transcripts from exons 1, 2, 3, and 4 (immediate-early 1 [ie1]). the expected ie72 and ie19 mrnas were detected, as well as two heretofore-uncharacterized transcripts designated ie17.5 and ie9. the ie72, ie19, and ie17.5 transcripts utilized the same 5'-splice site in exon 3. ie9 utilized a cryptic 5'-sp ... | 2004 | 15254190 |
| human cytomegalovirus-encoded g protein-coupled receptor us28 mediates smooth muscle cell migration through galpha12. | coupling of g proteins to ligand-engaged chemokine receptors is the paramount event in g-protein-coupled receptor signal transduction. previously, we have demonstrated that the human cytomegalovirus-encoded chemokine receptor us28 mediates human vascular smooth muscle cell (smc) migration in response to either rantes or monocyte chemoattractant protein 1. in this report, we identify the g proteins that couple with us28 to promote vascular smc migration and identify other signaling molecules that ... | 2004 | 15254210 |