Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nationwide survey of infectious bronchitis virus on layer farms in japan during 2015. | after accumulating data through a nationwide survey, we characterized the recent prevalences and geographic distributions of various genotypes of infectious bronchitis virus (ibv) on layer farms in japan. reverse transcription pcr analysis of fecal samples revealed the presence of the ibv nucleoprotein (n) gene on approximately 30% of the farms surveyed. n-gene detection rates were higher in the chugoku and kyushu regions than in the remaining surveyed regions. phylogenetic analysis of s1 gene s ... | 2020 | 32267128 |
| infectious bronchitis virus population structure defines immune response and protection. | a commercial arkansas (ark) delmarva poultry industry (dpi)-type vaccine and a more homogeneous population of that vaccine obtained previously through adaptation to chicken embryo kidney (cek) cells (cek-arkdpi) were used as a model to further understand the impact of population genetic structure on generation of immune responses and protection. in a first experiment, vaccinated chickens were challenged with an ibv ark99-type virulent strain (al/4614/98). despite extensive sequence similarity be ... | 2020 | 32267126 |
| limited protection conferred by recombinant newcastle disease virus expressing infectious bronchitis spike protein. | recombinant newcastle disease virus (ndv) lasota (ls) expressing secreted trimeric spike (s)-ectodomain (se) of infectious bronchitis virus (ibv) (rls/ibv.se) was developed and evaluated for protection conferred against ibv challenge. the ibv s-ectodomain protein, which is s excluding the transmembrane anchor and short cytoplasmic domain of s2, expressed from recombinant ls corresponds to an arkansas (ark)-type ibv. in a first experiment, chickens were primed at 1 day of age or primed at 1 day o ... | 2020 | 32267125 |
| cytokine responses in tracheas from major histocompatibility complex congenic chicken lines with distinct susceptibilities to infectious bronchitis virus. | the chicken major histocompatibility complex (mhc) b locus has been linked to resistance to infectious diseases. we have previously provided evidence that the mhc congenic chicken lines 331/b2 and 335/b19 differ in susceptibility to infectious bronchitis virus (ibv) strains m41 and arkdpi in in vivo challenge experiments. innate immune responses can be difficult to measure in vivo because they are nonspecific and can be triggered by environmental factors. in an attempt to address this issue, we ... | 2020 | 32267123 |
| intestinal tropism of an infectious bronchitis virus isolate not explained by spike protein binding specificity. | an infectious bronchitis virus (ibv) with an unusual enteric tropism (calent) was isolated from a california broiler flock exhibiting runting-stunting syndrome. ibv was detected in the small intestine, but not in the respiratory tract or kidney. during virus isolation in embryos, it did not replicate in chorioallantoic membrane (cam) but could be recovered from intestines. its s1 protein showed 93% amino acid sequence identity to a california variant isolated in 1999 (cal99). intestinal lesions ... | 2020 | 32267122 |
| multiple recombination events between field and vaccine strains resulted in the emergence of a novel infectious bronchitis virus with decreased pathogenicity and altered replication capacity. | in this study, we isolated and identified 2 infectious bronchitis virus (ibv) strains from layer chickens soon after vaccination with the massachusetts-connecticut bivalent vaccine (conn) and h120 and 4/91 booster vaccines in china in 2011. the results of cross-virus-neutralization tests and phylogenetic analysis of the s1 subunit of spike gene of these vaccine strains and other reference strains showed that strain ljl/110302 was of gi-19 lineage, whereas lln/111169 was of the gi-1 lineage of th ... | 2020 | 32241473 |
| genetic characterization of infectious bronchitis viruses in thailand, 2014-2016: identification of a novel recombinant variant. | infectious bronchitis (ib) causes severe economic losses to the poultry industry worldwide owing to frequent emergence of novel infectious bronchitis virus (ibv) variants, which potentially affect the effectiveness of the currently used ibv vaccine. therefore, continuous monitoring of ibv genotypes and lineages recently circulating in chickens worldwide is essential. in this study, we characterized the complete s1 gene from 120 ibvs circulating in chickens in thailand from 2014 to 2016. phylogen ... | 2020 | 32241468 |
| genetic sequence changes related to the attenuation of avian infectious bronchitis virus strain tw2575/98. | the attenuated avian infectious bronchitis virus (ibv), derived from a wild strain (tw2575/98w) in chicken embryos after 75 passages, is designed as a commercial vaccine strain (tw2575/98vac) to control the disease in taiwan. the differences in viral infectivity, replication efficiency, and genome sequences between tw2575/98w and tw2575/98vac were determined and compared. tw2575/98vac caused earlier death of chicken embryos and had higher viral replication efficiency. thirty amino acid substitut ... | 2020 | 32232712 |
| n-glycosylation of infectious bronchitis virus m41 spike determines receptor specificity. | infection of chicken coronavirus infectious bronchitis virus (ibv) is initiated by binding of the viral heavily n-glycosylated attachment protein spike to the alpha-2,3-linked sialic acid receptor neu5ac. previously, we have shown that n-glycosylation of recombinantly expressed receptor binding domain (rbd) of the spike of ibv-m41 is of critical importance for binding to chicken trachea tissue. here we investigated the role of n-glycosylation of the rbd on receptor specificity and virus replicat ... | 2020 | 32213247 |
| pathogenicity and genome changes in qx-like infectious bronchitis virus during continuous passaging in embryonated chicken eggs. | infectious bronchitis (ib) remains a major problem in the global poultry industry despite the many available vaccines. live attenuated vaccines are the most effective means of preventing ib and are traditionally generated by serial passaging of a wild strain in embryonated chicken eggs. in this study, the sz isolate of the qx-like infectious bronchitis virus (ibv) was continuously passaged in chicken embryos for 250 passages. we compared the pathogenicity of different passages (sz50, sz100, sz15 ... | 2020 | 32142742 |
| a novel low virulent respiratory infectious bronchitis virus originating from the recombination of qx, tw and 4/91 genotype strains in china. | in china, variants of infectious bronchitis virus (ibv) evolve continually and diverse recombinant strains have been reported. here, an ibv strain, designated as ck/ch/ljx/2017/07 (referred as jx17) was isolated from chicken vaccinated with h120 and 4/91 in jiangxi, china, in 2017. sequence analysis reveals of the s1 gene of jx17 the highest nucleotide identity of 98.15% with that of gi-7 genotype tw2575/98 strain. furthermore, whole genome analysis among jx17 and other 18 ibv strains demonstrat ... | 2020 | 32122588 |
| complete genome sequence of avian coronavirus strain ga08 (gi-27 lineage). | avian coronavirus, also known as infectious bronchitis virus, is a highly contagious respiratory pathogen of chickens that is responsible for major economic losses to the poultry industry around the globe. here, we report the complete genome sequence of strain ga08 of the gi-27 lineage, isolated from a fecal sample from a broiler chicken collected in georgia in 2015. | 2020 | 32107296 |
| 16s rrna gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus. | infectious bronchitis virus (ibv), a member of the coronaviridae family, causes serious losses to the poultry industry. intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading pathogens. the aim of this study was to investigate the link between the intestinal microbiome and nephropathogenic ibv (nibv) infection. initially, chickens were randomly distributed into 2 groups: the normal group (inc) and the infected group (iibv). t ... | 2020 | 32103130 |