Publications

characterization of lethal zika virus infection in ag129 mice.	mosquito-borne zika virus (zikv) typically causes a mild and self-limiting illness known as zika fever, which often is accompanied by maculopapular rash, headache, and myalgia. during the current outbreak in south america, zikv infection during pregnancy has been hypothesized to cause microcephaly and other diseases. the detection of zikv in fetal brain tissue supports this hypothesis. because human infections with zikv historically have remained sporadic and, until recently, have been limited t ...	2016	27093158
microcephaly and zika virus: neonatal neuroradiological aspects.	the aim of this study is to describe some radiological features in the newborns with microcephaly caused by zika virus infection during pregnancy.	2016	27080092
clinical features and neuroimaging (ct and mri) findings in presumed zika virus related congenital infection and microcephaly: retrospective case series study.	to report radiological findings observed in computed tomography (ct) and magnetic resonance imaging (mri) scans of the first cases of congenital infection and microcephaly presumably associated with the zika virus in the current brazilian epidemic.	2016	27075009
a cost-effectiveness tool for informing policies on zika virus control.	as zika virus continues to spread, decisions regarding resource allocations to control the outbreak underscore the need for a tool to weigh policies according to their cost and the health burden they could avert. for example, to combat the current zika outbreak the us president requested the allocation of $1.8 billion from congress in february 2016.	2016	27205899
time to empower release of insects carrying a dominant lethal and wolbachia against zika.	ridl (release of insects with dominant lethality) and wolbachia are 2 potentially powerful tools in the fight against zika, but their technological advancement is being hampered by policy barriers. in this study, we discuss what could be done to overcome these regulatory deadlocks.	2016	27419175
estimated zika virus importations to europe by travellers from brazil.	given the interconnectivity of brazil with the rest of the world, zika virus (zikv) infections have the potential to spread rapidly around the world via viremic travellers. the extent of spread depends on the travel volume and the endemicity in the exporting country. in the absence of reliable surveillance data, we did mathematical modelling to estimate the number of importations of zikv from brazil into europe.	2016	27193266
personal protection of permethrin-treated clothing against aedes aegypti, the vector of dengue and zika virus, in the laboratory.	the dengue and zika viruses are primarily transmitted by aedes aegypti mosquitoes, which are most active during day light hours and feed both in and outside of the household. personal protection technologies such as insecticide-treated clothing could provide individual protection. here we assessed the efficacy of permethrin-treated clothing on personal protection in the laboratory.	2016	27187593
the persistence and ecological impacts of a cyanobacterium genetically engineered to express mosquitocidal bacillus thuringiensis toxins.	the cyanobacterium anabaena pcc 7120#11 has been genetically engineered to act as a delivery vehicle for bacillus thuringiensis subspecies israelensis mosquitocidal toxins. to address ecological concerns about releasing this genetically engineered microorganism into the environment for mosquito larva control, the persistence and ecological impacts of pcc 7120#11 was evaluated using multi-species, standardized aquatic microcosms.	2016	27165108
the viral polymerase inhibitor 7-deaza-2'-c-methyladenosine is a potent inhibitor of in vitro zika virus replication and delays disease progression in a robust mouse infection model.	zika virus (zikv) is an emerging flavivirus typically causing a dengue-like febrile illness, but neurological complications, such as microcephaly in newborns, have potentially been linked to this viral infection. we established a panel of in vitro assays to allow the identification of zikv inhibitors and demonstrate that the viral polymerase inhibitor 7-deaza-2'-c-methyladenosine (7dma) efficiently inhibits replication. infection of ag129 (ifn-α/β and ifn-γ receptor knock-out) mice with zikv res ...	2016	27163257
zika virus, elevation, and transmission risk.	zika virus has appeared in the americas in the form of a major outbreak, and is now known to cause birth defects when pregnant women are infected. as a result, the centers for disease control and prevention issued travel guidelines, in the form of an elevational risk definition: destinations below 2000m are considered as at-risk.	2016	27280061
wolbachia modulates lipid metabolism in aedes albopictus mosquito cells.	certain strains of the intracellular endosymbiont wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (denv) by aedes mosquitoes, and the mechanisms responsible are still not well understood. direct infusion and liquid chromatography-fourier transform ion cyclotron resonance (ft-icr) mass spectrometry-based lipidomics analyses were conducted using aedes albopictus aa23 cells that were infected with the wmel and wmelpop strains of wolbachia in comparison to un ...	2016	26994075
assessing chikungunya risk in a metropolitan area of argentina through satellite images and mathematical models.	chikungunya fever is a viral disease that recently invaded the american continent. in america, it is transmitted mainly by the mosquito aedes aegypti, but aedes albopictus is the main vector in other regions of the world. this work estimates the risk of disease emergence and the corresponding population at risk for the case of a naive population in the metropolitan area of buenos aires, the capital city of argentina.	2016	26830024
whole-genome sequencing analysis from the chikungunya virus caribbean outbreak reveals novel evolutionary genomic elements.	chikungunya virus (chikv), an alphavirus and member of the togaviridae family, is capable of causing severe febrile disease in humans. in december of 2013 the asian lineage of chikv spread from the old world to the americas, spreading rapidly throughout the new world. given this new emergence in naïve populations we studied the viral genetic diversity present in infected individuals to understand how chikv may have evolved during this continuing outbreak.	2016	26807575
chikungunya: epidemiology.	chikungunya virus is a mosquito-borne alphavirus that causes fever and debilitating joint pains in humans. joint pains may last months or years. it is vectored primarily by the tropical and sub-tropical mosquito, aedes aegypti, but is also found to be transmitted by aedes albopictus, a mosquito species that can also be found in more temperate climates. in recent years, the virus has risen from relative obscurity to become a global public health menace affecting millions of persons throughout the ...	2016	26918158
prevalence of dengue and chikungunya virus infections in north-eastern tanzania: a cross sectional study among participants presenting with malaria-like symptoms.	in spite of increasing reports of dengue and chikungunya activity in tanzania, limited research has been done to document the general epidemiology of dengue and chikungunya in the country. this study aimed at determining the sero-prevalence and prevalence of acute infections of dengue and chikungunya virus among participants presenting with malaria-like symptoms (fever, headache, rash, vomit, and joint pain) in three communities with distinct ecologies of north-eastern tanzania.	2016	27112553
molecular characterisation of clinical isolates of chikungunya virus: a study from tertiary care hospitals in southern india.	indian ocean islands and india have experienced massive severe chikungunya outbreak from 2005 up till now and then chikungunya became epidemic in india. the mutations that occurred in e1 gene were responsible for increased infectivity, virulence and host adaptability. it is important to find out the genotype and its probable evolvement and novel mutations in the e1 gene reported during 2006-2009 from the current isolates, which may affect the local protein structure.	2016	27134872
forecasting chikungunya spread in the americas via data-driven empirical approaches.	chikungunya virus (chikv) is endemic to africa and asia, but the asian genotype invaded the americas in 2013. the fast increase of human infections in the american epidemic emphasized the urgency of developing detailed predictions of case numbers and the potential geographic spread of this disease.	2016	26928307
first imported case of chikungunya virus infection in a travelling canadian returning from the caribbean.	this is the first canadian case of chikungunya virus (chikv) infection reported in a traveller returning from the caribbean. following multiple mosquito bites in martinique island in january 2014, the patient presented with high fever, headaches, arthralgia on both hands and feet, and a rash on the trunk upon his return to canada. initial serological testing for dengue virus infection was negative. support therapy with nonsteroidal anti-inflammatory drugs was administered. the symptoms gradually ...	2016	27366163
aedes mosquitoes and zika virus infection: an a to z of emergence?		2016	26905027
vector competence of aedes aegypti in transmitting chikungunya virus: effects and implications of extrinsic incubation temperature on dissemination and infection rates.	aedes aegypti is a competent arthropod vector of chikungunya virus (chikv). the rate at which the virus disseminate in the vector is limited by temperature of their environment which can be an important determinant of geographical and seasonal limits to transmission by the arthropods in the tropics. this study investigated the vector competence of ae. aegypti for chikv at ambient temperature of 32 and 26 °c (coastal and western kenya respectively) reared at extrinsic incubation temperature (eit) ...	2016	27357190
development of neutralization assay using an egfp chikungunya virus.	chikungunya virus (chikv), a member of the alphavirus genus, is an important human emerging/re-emerging pathogen. currently, there are no effective antiviral drugs or vaccines against chikv infection. herein, we construct an infectious clone of chikv and an egfp reporter chikv (egfp-chikv) with an isolated strain (assigned to asian lineage) from chikv-infected patients. the egfp-chikv reporter virus allows for direct visualization of viral replication through the levels of egfp expression. using ...	2016	27367716
isolation of infective zika virus from urine and saliva of patients in brazil.	zika virus (zikv) is an emergent threat provoking a worldwide explosive outbreak. since january 2015, 41 countries reported autochthonous cases. in brazil, an increase in guillain-barré syndrome and microcephaly cases was linked to zikv infections. a recent report describing low experimental transmission efficiency of its main putative vector, ae. aegypti, in conjunction with apparent sexual transmission notifications, prompted the investigation of other potential sources of viral dissemination. ...	2016	27341420
seroprevalence of anti-chikungunya virus antibodies in children and adults in managua, nicaragua, after the first chikungunya epidemic, 2014-2015.	chikungunya is a viral disease transmitted by aedes aegypti and ae. albopictus mosquitoes. in late 2013, chikungunya virus (chikv) was introduced into the caribbean island of st. martin. since then, approximately 2 million chikungunya cases have been reported by the pan american health organization, and most countries in the americas report autochthonous transmission of chikv. in nicaragua, the first imported case was described in july 2014 and the first autochthonous case in september 2014. her ...	2016	27322692
antiviral hammerhead ribozymes are effective for developing transgenic suppression of chikungunya virus in aedes aegypti mosquitoes.	the chikungunya virus (chikv) is an emerging pathogen with widespread distribution in regions of africa, india, and asia that threatens to spread into temperate climates with the introduction of its major vector, aedes albopictus. chikv causes a disease frequently misdiagnosed as dengue fever, with potentially life-threatening symptoms that can result in a longer-term debilitating arthritis. the increasing risk of spread from endemic regions via human travel and commerce and the current absence ...	2016	27294950
dengue vaccine: considerations before rollout in colombia.		2016	27280803
novel lesions of bones and joints associated with chikungunya virus infection in two mouse models of disease: new insights into disease pathogenesis.	chikungunya virus is an arbovirus spread predominantly by aedes aegypti and ae. albopictus mosquitoes, and causes debilitating arthralgia and arthritis. while these are common manifestations during acute infection and it has been suggested they can recur in patients chronically, gaps in knowledge regarding the pathogenesis still exist. two established mouse models were utilized (adult irf 3/7 -/- -/- and wild-type c57bl/6j mice) to evaluate disease manifestations in bones and joints at various t ...	2016	27182740
identification and characterization of two novel rna viruses from anopheles gambiae species complex mosquitoes.	mosquitoes of the anopheles gambiae complex display strong preference for human bloodmeals and are major malaria vectors in africa. however, their interaction with viruses or role in arbovirus transmission during epidemics has been little examined, with the exception of o'nyong-nyong virus, closely related to chikungunya virus. deep-sequencing has revealed different rna viruses in natural insect viromes, but none have been previously described in the anopheles gambiae species complex. here, we d ...	2016	27138938
leukemoid reaction in chikungunya fever.	chikungunya is a viral illness caused by an arbovirus which is transmitted by aedes mosquito. fever and polyarthralgia are hallmark of this viral illness. viral infections are generally associated with leucopenia and bacterial infections with leukocytosis. leukemoid reaction (lr) is defined by reactive increase in leukocyte count of more than 50,000/cu mm with increase in mature leukocytes on peripheral blood. leukocytosis is common in chikungunya but leukemoid reaction has not been reported in ...	2016	27437276
the genetic basis of host preference and resting behavior in the major african malaria vector, anopheles arabiensis.	malaria transmission is dependent on the propensity of anopheles mosquitoes to bite humans (anthropophily) instead of other dead end hosts. recent increases in the usage of long lasting insecticide treated nets (llins) in africa have been associated with reductions in highly anthropophilic and endophilic vectors such as anopheles gambiae s.s., leaving species with a broader host range, such as anopheles arabiensis, as the most prominent remaining source of transmission in many settings. an. arab ...	2016	27631375
bacterial larvicide, bacillus thuringiensis israelensis strain am 65-52 water dispersible granule formulation impacts both dengue vector, aedes aegypti (l.) population density and disease transmission in cambodia.	a multi-phased study was conducted in cambodia from 2005-2011 to measure the impact of larviciding with the bacterial larvicide, bacillus thuringiensis israelensis (bti), a water dispersible granule (wg) formulation on the vector, aedes aegypti (l.) and the epidemiology. in our studies, all in-use containers were treated at 8 g/1000 l, including smaller containers and animal feeders which were found to contribute 23% of ae aegypti pupae. the treated waters were subjected to routine water exchang ...	2016	27627758
zika: an old virus with a new face.	zika virus is a mosquito-borne flavivirus that represents a public health emergency at the ongoing epidemic. this obscure virus was limited to sporadic cases in africa and asia, until the emergence of zika virus in brazil in 2015, when it rapidly spread throughout the americas. most zika virus infections are subclinical or characterized by mild febrile illness. however, neurological complications, including guillain-barré syndrome in adults, and congenital anomalies, including microcephaly in ba ...	2016	27703544
isolation of zika virus imported from tonga into australia.	the globally emergent zika virus (zikv) is a threat to australia, given the number of imported cases from epidemic regions and the presence of competent mosquito vectors. we report the isolation of zikv from a female traveler who recently returned from tonga to brisbane, queensland, australia in 2016.	2016	27679739
use of transgenic aedes aegypti in brazil: risk perception and assessment.	the ox513a strain of aedes aegypti, which was developed by the british company oxitec, expresses a self-limiting transgene that prevents larvae from developing to adulthood. in april 2014, the brazilian national technical commission on biosafety completed a risk assessment of ox513a and concluded that the strain did not present new biological risks to humans or the environment and could be released in brazil. at that point, brazil became the first country to approve the unconstrained release of ...	2016	27843167
an ecological assessment of the pandemic threat of zika virus.	the current outbreak of zika virus poses a severe threat to human health. while the range of the virus has been cataloged growing slowly over the last 50 years, the recent explosive expansion in the americas indicates that the full potential distribution of zika remains uncertain. moreover, many studies rely on its similarity to dengue fever, a phylogenetically closely related disease of unknown ecological comparability. here we compile a comprehensive spatially-explicit occurrence dataset from ...	2016	27564232
complete genome sequences of three historically important, spatiotemporally distinct, and genetically divergent strains of zika virus: mr-766, p6-740, and prvabc-59.	here, we report the 10,807-nucleotide-long consensus rna genome sequences of three spatiotemporally distinct and genetically divergent zika virus strains, with the functionality of their genomic sequences substantiated by reverse genetics: mr-766 (african lineage, uganda, 1947), p6-740 (asian lineage, malaysia, 1966), and prvabc-59 (asian lineage-derived american strain, puerto rico, 2015).	2016	27540058
zika virus: a call to action for physicians in the era of climate change.	in february 2016, the world health organization declared the mosquito-borne zika virus to be a "public health emergency of international concern" as the disease linked to thousands of birth defects in brazil spreads rapidly. the distribution of the aedes mosquitos has drastically increased over the past few decades, which have been the hottest decades on earth in more than 1000 years based on climate proxy measures. although a combination of factors explains the current zika virus outbreak, it's ...	2016	27617189
the importance of male body size on sperm uptake and usage, and female fecundity in aedes aegypti and aedes albopictus.	adult mosquito density is a critical factor in the transmission of arboviruses by container aedes spp. mosquitoes. female fecundity drives population growth, and therefore contributes to adult mosquito density. previous studies have focused on female body size as the major determinant of fecundity, paying little attention to male condition. in this study, we examined the effects of male body size on the abundance of sperm in spermatheca, depletion of sperm over time, and female fecundity.	2016	27519588
the critical role of early dengue surveillance and limitations of clinical reporting - implications for non-endemic countries.	the increasing dengue burden and epidemic severity worldwide have highlighted the need to improve surveillance. in non-endemic areas such as taiwan, where outbreaks start mostly with imported cases from southeast asia, a closer examination of surveillance dynamics to detect cases early is necessary. to evaluate problems with dengue surveillance and investigate the involvement of different factors at various epidemic stages, we investigated 632 laboratory-confirmed indigenous dengue cases in kaoh ...	2016	27501302
highly divergent dengue virus type 1 genotype sets a new distance record.	dengue viruses (denvs) are the leading cause of mosquito-borne viral disease of humans. they exist in both endemic and sylvatic ecotypes. in 2014, a viremic patient who had recently visited the rainforests of brunei returned to australia displaying symptoms consistent with denv infection. a unique denv strain was subsequently isolated from the patient, which we propose belongs to a new genotype within denv serotype 1 (denv-1). bayesian evolutionary phylogenetic analysis suggests that the putativ ...	2016	26924208
zika virus infection: new threat in global health.		2016	26955231
larvicidal potential of the halogenated sesquiterpene (+)-obtusol, isolated from the alga laurencia dendroidea j. agardh (ceramiales: rhodomelaceae), against the dengue vector mosquito aedes aegypti (linnaeus) (diptera: culicidae).	dengue is considered a serious public health problem in many tropical regions of the world including brazil. at the moment, there is no viable alternative to reduce dengue infections other than controlling the insect vector, aedes aegypti linnaeus. in the continuing search for new sources of chemicals targeted at vector control, natural products are a promising alternative to synthetic pesticides. in our work, we investigated the toxicity of a bioactive compound extracted from the red alga laure ...	2016	26821032
anticipating the international spread of zika virus from brazil.		2016	26777915
the zika pandemic - a perfect storm?		2016	26991663
zika: structuring the european research response.	studies are needed to assess #zika virus risks and to develop diagnostic tests http://ow.ly/zaozw.	2016	27730165
on the seasonal occurrence and abundance of the zika virus vector mosquito aedes aegypti in the contiguous united states.	an ongoing zika virus pandemic in latin america and the caribbean has raised concerns that travel-related introduction of zika virus could initiate local transmission in the united states (u.s.) by its primary vector, the mosquito aedes aegypti.	2016	27066299
zika virus outbreak: 'a perfect storm'.		2016	26956448
differential susceptibilities of aedes aegypti and aedes albopictus from the americas to zika virus.	since the major outbreak in 2007 in the yap island, zika virus (zikv) causing dengue-like syndromes has affected multiple islands of the south pacific region. in may 2015, the virus was detected in brazil and then spread through south and central america. in december 2015, zikv was detected in french guiana and martinique. the aim of the study was to evaluate the vector competence of the mosquito spp. aedes aegypti and aedes albopictus from the caribbean (martinique, guadeloupe), north america ( ...	2016	26938868
the expanding spectrum of modes of transmission of zika virus: a global concern.		2016	26939897
zika virus in a traveler returning to china from caracas, venezuela, february 2016.		2016	27070735
travel volume to the united states from countries and u.s. territories with local zika virus transmission.	air, land, and sea transportation can facilitate rapid spread of infectious diseases. in may 2015 the pan american health organization (paho) issued an alert regarding the first confirmed zika virus infection in brazil. as of march 8, 2016, the u.s. centers for disease control and prevention (cdc) had issued travel notices for 33 countries and 3 u.s. territories with local zika virus transmission.	2016	27990321
zika: what we do and do not know based on the experiences of brazil.	zika virus, which was first discovered in 1947, has become a global threat to human health as it is rapidly spreading through latin america, the caribbean, the us and asia, after causing a large outbreak in the northeast region of brazil in 2015. there is ample evidence to support that zika virus is associated with neurological complications such as microcephaly. the review aims to provide an overview on the complex issues involved in the emergence of zika virus's neurological disorders and to d ...	2016	27283140
using phenomenological models to characterize transmissibility and forecast patterns and final burden of zika epidemics.	the world health organization declared the ongoing zika virus (zikv) epidemic in the americas a public health emergency of international concern on february 1, 2016. zikv disease in humans is characterized by a "dengue-like" syndrome including febrile illness and rash. however, zikv infection in early pregnancy has been associated with severe birth defects, including microcephaly and other developmental issues. mechanistic models of disease transmission can be used to forecast trajectories and l ...	2016	27366586
zika in the united states of america and a fateful 1969 decision.	the united states gulf coast's current risk to zika transmitted by aedes aegypti mosquitoes can be traced back to some important federal health policy decisions made during the 1960s.	2016	27227971
wolbachia blocks currently circulating zika virus isolates in brazilian aedes aegypti mosquitoes.	the recent association of zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the zika vector, aedes aegypti mosquitoes. wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. here we report that aedes aegypti harboring wolbachia are highly resistant to infection with two currently circulating zika virus isolates from the recent brazilian epidemic. wolbac ...	2016	27156023
a sequence of flushing and drying of breeding habitats of aedes aegypti (l.) prior to the low dengue season in singapore.	in dengue-endemic areas, transmission shows both a seasonal and interannual variability. to investigate how rainfall impacts dengue seasonality in singapore, we carried out a longitudinal survey in the geylang neighborhood from august 2014 to august 2015. the survey comprised of twice-weekly random inspections to outdoor breeding habitats and continuous monitoring for positive ones. in addition, observations of rainstorms were collected. out of 6824 inspected habitats, 67 contained aedes aegypti ...	2016	27459322
zika - an emerging global threat.		2016	27822403
gut bacteria differentially affect egg production in the anautogenous mosquito aedes aegypti and facultatively autogenous mosquito aedes atropalpus (diptera: culicidae).	aedes aegypti and a. atropalpus are related mosquitoes that differ reproductively. aedes aegypti must blood-feed to produce eggs (anautogenous) while a. atropalpus always produces a first clutch of eggs without blood-feeding (facultatively autogenous). we recently characterized the gut microbiota of a. aegypti and a. atropalpus that were reared identically in the laboratory. here, we assessed the effects of specific members of the gut microbiota in a. aegypti and a. atropalpus on female fitness ...	2016	27363842
resistance status to the insecticides temephos, deltamethrin, and diflubenzuron in brazilian aedes aegypti populations.	insecticides are still largely applied in public health to control disease vectors. in brazil, organophosphates (op) and pyrethroids (py) are used against aedes aegypti for years. since 2009 insect growth regulators (igr) are also employed in the control of larvae. we quantified resistance to temephos (op), deltamethrin (py), and diflubenzuron (igr) of a. aegypti samples from 12 municipalities distributed throughout the country, collected between 2010 and 2012. high levels of resistance to neuro ...	2016	27419140
the current status of zika virus in southeast asia.	zika virus currently poses a global threat and is a major public health issue throughout latin america and the caribbean. however, zika virus infections in humans have also been observed in other regions, including southeast asia, where arboviral diseases are very common. in this study, we summarize the current status of zika virus in southeast asia. this review aims to provide an overview of the current situation and also to suggest ways of adequately managing the emergence of zika virus in sou ...	2016	27336445
the when and the where of zika epidemic potential in europe - an evidence base for public health preparedness.		2016	27333025
potential risk of dengue and chikungunya outbreaks in northern italy based on a population model of aedes albopictus (diptera: culicidae).	the rapid invasion and spread of aedes albopictus (skuse, 1894) within new continents and climatic ranges has created favorable conditions for the emergence of tropical arboviral diseases in the invaded areas. we used mosquito abundance data from 2014 collected across ten sites in northern italy to calibrate a population model for aedes albopictus and estimate the potential of imported human cases of chikungunya or dengue to generate the condition for their autochthonous transmission in the abse ...	2016	27304211
prospects and challenges of crispr/cas genome editing for the study and control of neglected vector-borne nematode diseases.	neglected tropical diseases caused by parasitic nematodes inflict an immense health and socioeconomic burden throughout much of the developing world. current estimates indicate that more than two billion people are infected with nematodes, resulting in the loss of 14 million disability-adjusted life years per annum. although these parasites cause significant mortality, they primarily cause chronic morbidity through a wide range of severe clinical ailments. treatment options for nematode infectio ...	2016	27300487
metarhizium brunneum blastospore pathogenesis in aedes aegypti larvae: attack on several fronts accelerates mortality.	aedes aegypti is the vector of a wide range of diseases (e.g. yellow fever, dengue, chikungunya and zika) which impact on over half the world's population. entomopathogenic fungi such as metarhizium anisopliae and beauveria bassiana have been found to be highly efficacious in killing mosquito larvae but only now are the underlying mechanisms for pathogenesis being elucidated. recently it was shown that conidia of m. anisopliae caused stress induced mortality in ae. aegypti larvae, a different mo ...	2016	27389584
detecting guillain-barré syndrome caused by zika virus using systems developed for polio surveillance.		2016	27708476
efficacy of ulv and thermal aerosols of deltamethrin for control of aedes albopictus in nice, france.	ultra-low volume (ulv) insecticidal aerosols dispensed from vehicle-mounted cold-foggers are widely considered the method of choice for control of aedes aegypti and ae. albopictus during outbreaks of dengue and chikungunya and, more recently, zika. nevertheless, their effectiveness has been poorly studied, particularly in europe. nearly all published studies of ulv efficacy are bio-assays based on the mortality of caged mosquitoes. in our study we preferred to monitor the direct impact of treatm ...	2016	27881181
assessment of icount software, a precise and fast egg counting tool for the mosquito vector aedes aegypti.	widespread in the tropics, the mosquito aedes aegypti is an important vector of many viruses, posing a significant threat to human health. vector monitoring often requires fecundity estimation by counting eggs laid by female mosquitoes. traditionally, manual data analyses have been used but this requires a lot of effort and is the methods are prone to errors. an easy tool to assess the number of eggs laid would facilitate experimentation and vector control operations.	2016	27863526
hiv and zika: when will we be able to end these epidemics?		2016	27846847
zika virus may affect the universal two-child policy: a new challenge for china.		2016	27832058
pyriproxyfen and the microcephaly epidemic in brazil - an ecological approach to explore the hypothesis of their association.	the microcephaly epidemic in brazil generated intense debate regarding its causality, and one hypothesised cause of this epidemic, now recognised as congenital zika virus syndrome, was the treatment of drinking water tanks with pyriproxyfen to control aedes aegypti larvae. we present the results of a geographical analysis of the association between the prevalence of microcephaly confirmed by fenton growth charts and the type of larvicide used in the municipalities that were home to the mothers o ...	2016	27812601
near-infrared spectroscopy, a rapid method for predicting the age of male and female wild-type and wolbachia infected aedes aegypti.	estimating the age distribution of mosquito populations is crucial for assessing their capacity to transmit disease and for evaluating the efficacy of available vector control programs. this study reports on the capacity of the near-infrared spectroscopy (nirs) technique to rapidly predict the ages of the principal dengue and zika vector, aedes aegypti. the age of wild-type males and females, and males and females infected with wmel and wmelpop strains of wolbachia pipientis were characterized u ...	2016	27768689
analyzing climate variations at multiple timescales can guide zika virus response measures.	the emergence of zika virus (zikv) in latin america and the caribbean in 2014-2016 occurred during a period of severe drought and unusually high temperatures, conditions that have been associated with the 2015-2016 el niño event, and/or climate change; however, no quantitative assessment has been made to date. analysis of related flaviviruses transmitted by the same vectors suggests that zikv dynamics are sensitive to climate seasonality and longer-term variability and trends. a better understan ...	2016	27716414
genetic variability and spatial distribution in small geographic scale of aedes aegypti (diptera: culicidae) under different climatic conditions in northeastern brazil.	the study of the genetic structure of aedes aegypti is essential to understanding their population dynamics as well as for the analysis of factors responsible for their resistance and ecological adaptation. the use of molecular markers in identifying differences amongst populations of ae. aegypti in different geographical areas as well as the temporal variation of the vector populations has contributed to the improvement of vector control strategies. the present study aims to determine the genet ...	2016	27716392
first detection of natural infection of aedes aegypti with zika virus in brazil and throughout south america.	zika virus (zikv) has caused a major epidemic in brazil and several other american countries. zikv is an arbovirus whose natural vectors during epidemics have been poorly determined. in this study, 1,683 mosquitoes collected in the vicinity of zikv suspected cases in rio de janeiro, brazil, from june 2015 to may 2016 were screened for natural infection by using molecular methods. three pools of aedes aegypti were found with the zikv genome, one of which had only one male. this finding supports t ...	2016	27706382
yellow fever-more a policy and planning problem than a biological one.		2016	27479749
oviposition ecology and species composition of aedes spp. and aedes aegypti dynamics in variously urbanized settings in arbovirus foci in southeastern côte d'ivoire.	aedes mosquito-transmitted outbreaks of dengue and yellow fever have been reported from rural and urban parts of côte d'ivoire. the present study aimed at assessing aedes spp. oviposition ecology in variously urbanized settings within arbovirus foci in southeastern côte d'ivoire.	2016	27682270
vector competence of french polynesian aedes aegypti and aedes polynesiensis for zika virus.	in 2013-2014, french polynesia experienced for the first time a zika outbreak. two aedes mosquitoes may have contributed to zika virus (zikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito.	2016	27654962
assessment of local mosquito species incriminates aedes aegypti as the potential vector of zika virus in australia.	within the last 10 years zika virus (zikv) has caused unprecedented epidemics of human disease in the nations and territories of the western pacific and south america, and continues to escalate in both endemic and non-endemic regions. we evaluated the vector competence of australian mosquitoes for zikv to assess their potential role in virus transmission.	2016	27643685
insights into zika virus history, human health effects, and control measures.		2016	27679523
contraceptive sales in the setting of the zika virus epidemic.	has there been any influence of the zika virus (zikv) outbreak on the sales of contraceptive methods in brazil?	2016	27932442
perspectives on the role of mobility, behavior, and time scales in the spread of diseases.	the dynamics, control, and evolution of communicable and vector-borne diseases are intimately connected to the joint dynamics of epidemiological, behavioral, and mobility processes that operate across multiple spatial, temporal, and organizational scales. the identification of a theoretical explanatory framework that accounts for the pattern regularity exhibited by a large number of host-parasite systems, including those sustained by host-vector epidemiological dynamics, is but one of the challe ...	2016	27965394
a historic report of zika in mozambique: implications for assessing current risk.		2016	27930650
public health interventions for aedes control in the time of zikavirus- a meta-review on effectiveness of vector control strategies.	there is renewed interest in effective measures to control zika and dengue vectors. a synthesis of published literature with a focus on the quality of evidence is warranted to determine the effectiveness of vector control strategies.	2016	27926934
the self-regulation model of illness: comparison between zika and dengue and its application to predict mosquito prevention behaviours in malaysia, a dengue-endemic country.	perceptions about illnesses may influence self-care and preventive health behaviours. illness perceptions of the zika virus (zikv) infection were investigated under the framework of the self-regulation model of illness. illness perception differences between zikv and dengue fever were also examined. lastly, associations between illness perceptions of zikv with mosquito prevention practices were studied. samples were drawn from landline telephone numbers using computer-assisted telephone intervie ...	2016	27929451
comparative genomics of beauveria bassiana: uncovering signatures of virulence against mosquitoes.	entomopathogenic fungi such as beauveria bassiana are promising biological agents for control of malaria mosquitoes. indeed, infection with b. bassiana reduces the lifespan of mosquitoes in the laboratory and in the field. natural isolates of b. bassiana show up to 10-fold differences in virulence between the most and the least virulent isolate. in this study, we sequenced the genomes of five isolates representing the extremes of low/high virulence and three rna libraries, and applied a genome c ...	2016	27905873
tracking insecticide resistance in mosquito vectors of arboviruses: the worldwide insecticide resistance network (win).		2016	27906961
human igg antibody response to aedes nterm-34kda salivary peptide, an epidemiological tool to assess vector control in chikungunya and dengue transmission area.	arboviral diseases are an important public health concerns. vector control remains the sole strategy to fight against these diseases. because of the important limits of methods currently used to assess human exposure to aedes mosquito bites, much effort is being devoted to develop new indicators. recent studies have reported that human antibody (ab) responses to aedes aegypti nterm-34kda salivary peptide represent a promising biomarker tool to evaluate the human-aedes contact. the present study ...	2016	27906987
comparative mt genomics of the tipuloidea (diptera: nematocera: tipulomorpha) and its implications for the phylogeny of the tipulomorpha.	a traditionally controversial taxon, the tipulomorpha has been frequently discussed with respect to both its familial composition and relationships with other nematocera. the interpretation of internal relationships within the tipuloidea, which include the tipulidae sensu stricto, cylindrotomidae, pediciidae and limoniidae, is also problematic. we sequenced the first complete mitochondrial (mt) genome of symplecta hybrida (meigen, 1804), which belongs to the subfamily chioneinae of family limoni ...	2016	27341029
a review of bioinsecticidal activity of solanaceae alkaloids.	only a small percentage of insect species are pests. however, pest species cause significant losses in agricultural and forest crops, and many are vectors of diseases. currently, many scientists are focused on developing new tools to control insect populations, including secondary plant metabolites, e.g., alkaloids, glycoalkaloids, terpenoids, organic acids and alcohols, which show promise for use in plant protection. these compounds can affect insects at all levels of biological organization, b ...	2016	26938561
proceedings of the ix international symposium on phlebotomine sandflies (isops ix), reims, france, june 28th-july 1st, 2016.		2016	27677701
molecular identification of mosquitoes (diptera: culicidae) in southeastern australia.	dna barcoding is a modern species identification technique that can be used to distinguish morphologically similar species, and is particularly useful when using small amounts of starting material from partial specimens or from immature stages. in order to use dna barcoding in a surveillance program, a database containing mosquito barcode sequences is required. this study obtained cytochrome oxidase i (coi) sequences for 113 morphologically identified specimens, representing 29 species, six trib ...	2016	27217948
consolidating tactical planning and implementation frameworks for integrated vector management in uganda.	integrated vector management (ivm) is the recommended approach for controlling some vector-borne diseases (vbd). in the face of current challenges to disease vector control, ivm is vital to achieve national targets set for vbd control. though global efforts, especially for combating malaria, now focus on elimination and eradication, ivm remains useful for uganda which is principally still in the control phase of the malaria continuum. this paper outlines the processes undertaken to consolidate t ...	2016	27074809
restriction to gene flow is associated with changes in the molecular basis of pyrethroid resistance in the malaria vector anopheles funestus.	resistance to pyrethroids, the sole insecticide class recommended for treating bed nets, threatens the control of major malaria vectors, including anopheles funestus effective management of resistance requires an understanding of the dynamics and mechanisms driving resistance. here, using genome-wide transcription and genetic diversity analyses, we show that a shift in the molecular basis of pyrethroid resistance in southern african populations of this species is associated with a restricted gen ...	2016	28003461
salivary gland proteome during adult development and after blood feeding of female anopheles dissidens mosquitoes (diptera: culicidae).	understanding changes in mosquito salivary proteins during the time that sporozoite maturation occurs and after blood feeding may give information regarding the roles of salivary proteins during the malarial transmission. anopheles dissidens (formerly anopheles barbirostris species a1) is a potential vector of plasmodium vivax in thailand. in this study, analyses of the proteomic profiles of female an. dissidens salivary glands during adult development and after blood feeding were carried out us ...	2016	27669021
mosquito host choices on livestock amplifiers of rift valley fever virus in kenya.	animal hosts may vary in their attraction and acceptability as components of the host location process for assessing preference, and biting rates of vectors and risk of exposure to pathogens. however, these parameters remain poorly understood for mosquito vectors of the rift valley fever (rvf), an arboviral disease, and for a community of mosquitoes.	2016	27036889
towards a proteomic catalogue and differential annotation of salivary gland proteins in blood fed malaria vector anopheles culicifacies by mass spectrometry.	in order to understand the importance of functional proteins in mosquito behavior, following blood meal, a baseline proteomic dataset is essential for providing insights into the physiology of blood feeding. therefore, in this study as first step, in solution and 1-d electrophoresis digestion approach combined with tandem mass spectrometry (nano lc-ms/ms) and computational bioinformatics for data mining was used to prepare a baseline proteomic catalogue of salivary gland proteins of sugar fed an ...	2016	27602567
genome-wide identification and characterization of carboxypeptidase genes in silkworm (bombyx mori).	the silkworm (bombyx mori) is an economically-important insect that can secrete silk. carboxypeptidases have been found in various metazoan species and play important roles in physiological and biochemical reactions. here, we analyzed the silkworm genome database and characterized 48 carboxypeptidases, including 34 metal carboxypeptidases (bmmcp1-bmmcp34) and 14 serine carboxypeptidases (bmscp1-bmscp14), to better understand their diverse functions. compared to other insects, our results indicat ...	2016	27483237
malaria vector anopheles culicifacies sibling species differentiation using egg morphometry and morphology.	the malaria vector anopheles culicifacies (sensu lato) is an important malaria vector in southeast asia which comprises of five sibling species namely a, b, c, d and e. however, only a few forms have been identified as malaria vectors in various endemic countries. currently, for the first time egg morphometry and morphology has been used to differentiate the three known vector sibling species of anopheles culicifacies collected from malaria endemic madhya pradesh state of central india.	2016	27075571
entomological aspects and the role of human behaviour in malaria transmission in a highland region of the republic of yemen.	the republic of yemen has the highest incidence of malaria in the arabian peninsula, yet little is known of its vectors or transmission dynamics.	2016	26932794
mosquito oviposition behavior and vector control.	the burden of gene transfer from one mosquito generation to the next falls on the female and her eggs. the selection of an oviposition site that guarantees egg and larval survival is a critical step in the reproductive process. the dangers associated with ephemeral aquatic habitats, lengthy droughts, freezing winters, and the absence of larval nutrition makes careful oviposition site selection by a female mosquito extremely important. mosquito species exhibit a remarkable diversity of ovipositio ...	2016	27869724
identification of immunity-related genes in dialeurodes citri against entomopathogenic fungus lecanicillium attenuatum by rna-seq analysis.	dialeurodes citri is a major pest in citrus producing areas, and large-scale outbreaks have occurred increasingly often in recent years. lecanicillium attenuatum is an important entomopathogenic fungus that can parasitize and kill d. citri. we separated the fungus from corpses of d. citri larvae. however, the sound immune defense system of pests makes infection by an entomopathogenic fungus difficult. here we used rna sequencing technology (rna-seq) to build a transcriptome database for d. citri ...	2016	27644092