Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| improvement and evaluation of loop-mediated isothermal amplification for rapid detection of toxoplasma gondii infection in human blood samples. | loop-mediated isothermal amplification (lamp), an attractive dna amplification method, was developed as a valuable tool for the rapid detection of toxoplasma gondii. in this study, species-specific lamp primers were designed by targeting the af146527 sequence, which was a conserved sequence of 200- to 300-fold repetitive 529 bp fragment of t.gondii. lamp reaction system was optimized so that it could detect the minimal dna sample such as a single tachyzoite or 10 copies of recombinant plasmid. n ... | 2017 | 28056092 |
| antiparasitic activity of sulfur- and fluorine-containing bisphosphonates against trypanosomatids and apicomplexan parasites. | based on crystallographic data of the complexes 2-alkyl(amino)ethyl-1,1-bisphosphonates-trypanosoma cruzi farnesyl diphosphate synthase, some linear 1,1-bisphosphonic acids and other closely related derivatives were designed, synthesized and biologically evaluated against t. cruzi, the responsible agent of chagas disease and against toxoplasma gondii, the etiologic agent of toxoplasmosis and also towards the target enzymes farnesyl pyrophosphate synthase of t. cruzi (tcfpps) and t gondii (tgfpps ... | 2017 | 28054995 |
| first genetic characterization of toxoplasma gondii infection in common quails (coturnix coturnix) intended for human consumption in china. | toxoplasma gondii is widely distributed in humans and other animals including birds throughout the world. however, little is known of the molecular epidemiology and genotypes of t. gondii infecting quails in china. therefore, the present study was conducted to characterize t. gondii genotypes in common quails in china. during december 2014 to october 2015, a total of 390 muscle tissue samples of common quails were collected and the t. gondii b1 gene was amplified using a nested pcr, and the posi ... | 2017 | 28043887 |
| survival of toxoplasma gondii tachyzoites in simulated gastric fluid and cow's milk. | the protozoan parasite toxoplasma gondii is one of the most important food-related pathogens worldwide. besides contact to oocysts or ingestion of tissue cysts mainly by consumption of raw or undercooked meat from infected animals, raw milk is considered to be a risk factor and possible route of transmission for tachyzoites. this stage of the parasite is usually very sensitive to acidic ph and, therefore, considered unlikely to survive stomach passage. however, tachyzoites were shown to survive ... | 2017 | 28043380 |
| molecular detection and genotypic characterization of toxoplasma gondii in paraffin-embedded fetoplacental tissues of women with recurrent spontaneous abortion. | congenital toxoplasmosis is an important cause of spontaneous abortion worldwide. however, there is limited information on detection and genotypic characterization of toxoplasma gondii (t. gondii) in women with recurrent spontaneous abortion (rsa). the aim of this study is the molecular detection and genotypic characterization of t. gondii in formalin-fixed, paraffin-embedded fetoplacental tissues (ffpts) of women with rsa that have referred to the avicenna research institute in tehran, iran. | 2017 | 28042412 |
| validation of igg, igm multiplex plasmonic gold platform in french clinical cohorts for the serodiagnosis and follow-up of toxoplasma gondii infection. | we report the use of the multiplexed t. gondii igg, igm test on plasmonic gold (pgold) platform in the setting of t. gondii infection by analyzing 244 sera from nice, france (seroconversion, chronically infected, non-infected and newborns serum samples). results were compared with commercial tests for the detection of igg and igm and their overall clinical final interpretation of a complete serological profile. the igg and igm test results on the platform were in agreement in, respectively, 95% ... | 2017 | 28040304 |
| comparison of pcr assays targeting the multi-copy targets b1 gene and 529 bp repetitive element for detection of toxoplasma gondii in swine muscle. | the comparison of the sensitivities of two molecular assays designed to target the multi-copy sequences of the toxoplasma gondii genomic b1 region and 529 bp-re respectively, in detecting t. gondii in swine muscle was assessed. diaphragm pillars were obtained from 498 slaughtered pigs managed in intensive farms in central italy. genomic dna was extracted from the tissues and t. gondii-b1 and 529 bp-re sequences were amplified by specific pcr protocols. toxoplasma gondii dna was detected in 165 s ... | 2017 | 28040171 |
| toxoplasma gondii in domestic and wild animals from forest fragments of the municipality of natal, northeastern brazil. | toxoplasmosis stands out as a global disease that has felines as definitive hosts. in the municipality of natal, rio grande do norte state, brazil, two parks are notable for their ecological and social importance. this study aimed to investigate the presence of toxoplasma gondii in short hair cats, bats and small non-volant mammals in these two ecological reserves. altogether, biological samples were obtained from 154 mammals, 92 wild animals from both areas and 62 domestic cats of the parque da ... | 2017 | 25517529 |
| occurrence of anti-toxoplasma gondii antibodies in meat and dairy goat herds in rio grande do norte, brazil. | toxoplasmosis is caused by toxoplasma gondii, which is the main causative agent of abortion in small ruminants. goats are among the animals that are most susceptible to this protozoon, and the disease that it causes leads to significant economic losses and has implications for public health, since presence of the parasite in products of goat origin is one of the main sources of human infection. because of the significant economic impact, there is an urgent need to study the prevalence of t. gond ... | 2017 | 25517526 |
| genetic characterization of toxoplasma gondii isolates from eared doves (zenaida auriculata) in brazil. | eared doves (zenaida auriculata), which are common in urban, rural and wild areas in many regions of brazil, are frequently prey for domestic cats. therefore toxoplasma gondii isolates obtained from doves may reflect greater environmental diversity than those from other hosts. the aim of the present study was to evaluate t. gondii seroprevalence, isolate and genotype strains from z. auriculata. serum and tissue samples were collected from 206 doves for use in the modified agglutination test (mat ... | 2017 | 25517521 |
| toxoplasma gondii antibody profile in hiv-1-infected and uninfected pregnant women and the impact on congenital toxoplasmosis diagnosis in rio de janeiro, brazil. | compare the anti-t. gondii igg titer between hiv-1 infected and non hiv-1 infected pregnant women and report three cases of congenital toxoplasmosis resulting from reactivation of infection during pregnancy of hiv-1 infected women. | 2017 | 22552460 |
| recombinant rop2, rop4, gra4 and sag1 antigen-cocktails as possible tools for immunoprophylaxis of toxoplasmosis: what's next? | toxoplasmosis is a globally distributed foodborne zoonosis caused by a protozoan parasite toxoplasma gondii. usually asymptomatic in immunocompetent humans, toxoplasmosis is a serious clinical and veterinary problem often leading to lethal damage in an infected host. in order to overcome the exceptionally strong clinical and socio-economic impact of toxoplasma infection, the construction of an effective vaccine inducing full immunoprotection against the parasite is an urgent issue. in the last t ... | 2017 | 22892593 |
| novel fitc-labeled igy antibody: fluorescence imaging toxoplasma gondii in vitro. | toxoplasmosis is caused by t. gondii and can create serious health problems in humans and also worldwide economic harm. because of the clinical and veterinary importance of toxoplasmosis, its timely and accurate diagnosis has a major impact on disease-fighting strategies. t. gondii surface antigen 1 (sag1), an immunodominant-specific antigen, is often used as a diagnostic tool. therefore, the aim of this study was the optimization of novel fluorescein isothiocyanate (fitc) labeling of the sag1-s ... | 2017 | 28404997 |
| pathological findings in the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereutes procyonoides), with special emphasis on infectious and zoonotic agents in northern germany. | anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. the present study provides a detailed characterisation of the health status of the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereu ... | 2017 | 28399176 |
| anaplasma phagocytophilum, babesia microti, borrelia burgdorferi sensu lato, and toxoplasma gondii in ixodes ricinus (acari, ixodida) ticks collected from slowinski national park (northern poland). | 2017 | 28504439 | |
| detection and prevalence of protozoan parasites in ready-to-eat packaged salads on sale in italy. | to investigate the prevalence of protozoan contamination by giardia duodenalis, cryptosporidium spp., toxoplasma gondii and cyclospora cayetanensis, in 'ready to eat' (rte) salads on sale in italy, 648 packages were purchased from industrial and local brands. nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. microscopic examination of 864 slides detected cryptosporidium spp. but also blastocystis hominis and dientamoeba ... | 2017 | 28648295 |
| antibodies to various zoonotic pathogens detected in feral swine (sus scrofa) at abattoirs in texas, usa. | the zoonotic risk posed to employees by slaughtering feral swine (sus scrofa) at two abattoirs in texas was assessed by testing feral swine serum samples for exposure to influenza a virus, leptospira, trichinella spiralis, and toxoplasma gondii. blood was collected from a total of 376 feral swine between the two facilities during six separate collection periods in 2015. antibodies to one or more serovars of leptospira were identified in 48.9% of feral swine tested, with bratislava and pomona as ... | 2017 | 28686494 |
| serology and protein electrophoresis for evidence of exposure to 12 mink pathogens in free-ranging american mink (neovison vison) in argentina. | basic pathologic characteristics for farmed minks were previously reported worldwide. however, its status in the wild has not been studied in detail. | 2017 | 28635524 |
| serosurvey of anti- leptospira sp. and anti- toxoplasma gondii antibodies in capybaras and collared and white-lipped peccaries. | brazilian native species are reemerging as increasingly free-ranging populations. | 2017 | 28562764 |
| a retrospective survey into the presence of plasmodium spp. and toxoplasma gondii in archived tissue samples from new zealand raptors: new zealand falcons (falco novaeseelandiae), australasian harriers (circus approximans) and moreporks (ninox novaeseelandiae). | human colonisation of new zealand has resulted in the introduction of emerging diseases, such as avian malaria and toxoplasmosis, which arrived with their exotic avian and mammalian hosts. plasmodium spp. and toxoplasma gondii have a wide host range, and several species of endemic new zealand birds have developed a fatal disease following infection with either pathogen. however, no reports of either toxoplasmosis or avian malaria in new zealand raptors, namely, the new zealand falcons (falco nov ... | 2017 | 28660290 |
| experimental toxoplasmosis in japanese quails (coturnix coturnix japonica). | in this study, forming of experimental toxoplasmosis in quails; clinical, pathological, and serological determination of tissue lesions and bioassay techniques, which were aimed to compare them and determine pathogenesis. | 2017 | 28695827 |
| first report of toxoplasma gondii infection in common quails (coturnix coturnix) intended for human consumption in three provinces of northeastern china. | common quails are nutritious food and are also used as medicine in some parts of the world. until now, no information on toxoplasma gondii infection in common quails intended for human consumption in china is available. therefore, this study was carried out to determine the prevalence of t. gondii infection in common quails in six cities from shandong, liaoning, and jilin provinces, china. out of 620 common quails examined, 59 (9.52%) were seropositive for t. gondii by the modified agglutination ... | 2017 | 28437182 |
| survey for selected pathogens in wild pigs (sus scrofa) from guam, marianna islands, usa. | pigs (sus scrofa) were introduced to guam in the 1600's and are now present in high densities throughout the island. wild pigs are reservoirs for pathogens of concern to domestic animals and humans. exposure to porcine parvovirus, transmissible gastroenteritis, and leptospira interrogans has been documented in domestic swine but data from wild pigs are lacking. the close proximity of humans, domestic animals, and wild pigs, combined with the liberal hunting of wild pigs, results in frequent oppo ... | 2017 | 28622856 |
| high-resolution melting pcr assay, applicable for diagnostics and screening studies, allowing detection and differentiation of several babesia spp. infecting humans and animals. | the goal of the study was to design a single tube pcr test for detection and differentiation of babesia species in dna samples obtained from diverse biological materials. a multiplex, single tube pcr test was designed for amplification of approximately 400 bp region of the babesia 18s rrna gene. universal primers were designed to match dna of multiple babesia spp. and to have low levels of similarity to dna sequences of other intracellular protozoa and babesia hosts. the pcr products amplified f ... | 2017 | 28795223 |
| serological and molecular detection of toxoplasma gondii and babesia microti in the blood of rescued wild animals in gangwon-do (province), korea. | infections of toxoplasma gondii and babesia microti are reported in many wild animals worldwide, but information on their incidence and molecular detection in korean wild fields is limited. in this study, the prevalence of t. gondii and b. microti infection in blood samples of 5 animal species (37 chinese water deer, 23 raccoon dogs, 6 roe deer, 1 wild boar, and 3 eurasian badgers) was examined during 2008-2009 in gangwon-do (province), the republic of korea (=korea) by using serological and mol ... | 2017 | 28506045 |
| bartonella henselae as a cause of acute-onset febrile illness in cats. | at different time points spanning 6 months, three adopted feral flea-infested cats, residing in the household of a veterinary technician, became acutely anorexic, lethargic and febrile. enrichment blood culture/pcr using bartonella alpha proteobacteria growth medium (bapgm) confirmed initial infection with the same bartonella henselae genotype in all three cases. with the exception of anemia and neutropenia, complete blood counts, serum biochemical profiles and urinalysis results were within ref ... | 2017 | 28491382 |
| serological survey of toxoplasmosis, neosporosis and brucellosis among cattle herds in oyo state, south-western nigeria. | several zoonotic diseases are known to constitute great impediment to livestock management and production worldwide, especially in developing countries where control measures are largely non-existent. this study sets out to investigate the occurrence of toxoplasmosis, neosporosis and brucellosis among cattle herds in oyo state, southwest nigeria. | 2017 | 28670646 |
| evaluation of primitive ground water supplies as a risk factor for the development of major waterborne zoonosis in egyptian children living in rural areas. | endemic waterborne zoonosis frequently occurs in both developed and less developed countries. thus, bio-surveillance of waterborne zoonosis is a "necessity" for the implementation of effective preventive public health measures in egyptian rural areas. the primitive individual water supplies created by the rural agriculture population, primarily from ground water, usually maximize the customers' exposure to impurity pathogens via diffused humans and animal excreta or wastages. the current study a ... | 2017 | 28843417 |
| immunoprevalence to six waterborne pathogens in beachgoers at boquerón beach, puerto rico: application of a microsphere-based salivary antibody multiplex immunoassay. | waterborne infectious diseases are a major public health concern worldwide. few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. in this article, we applied a multiplex bead-based immunoassay capable of ... | 2017 | 28507984 |
| toxoplasma gondii maf1b binds the host cell mib complex to mediate mitochondrial association. | many diverse intracellular pathogens, such as legionella pneumophila, chlamydia psittaci, encephalitozoon sp., and toxoplasma gondii, manipulate and relocate host cell organelles, including mitochondria. toxoplasma tachyzoites use a secreted protein, mitochondrial association factor 1b (maf1b), to drive the association between the host mitochondria and the membrane of the parasitophorous vacuole, in which the parasites grow. the identity of the host partner in this interaction, however, has not ... | 2017 | 28567444 |
| tpl2 promotes innate cell recruitment and effector t cell differentiation to limit citrobacter rodentium burden and dissemination. | tumor progression locus 2 (tpl2) is a serine-threonine kinase that regulates th1 differentiation, secretion of the inflammatory cytokine ifnγ, and host defense against the intracellular pathogens toxoplasma gondii, listeria monocytogenes, and mycobacterium tuberculosis however, there is relatively little known about the contribution of tpl2 to th17 differentiation and immune cell function during infection with an extracellular pathogen. the goal of this study was to determine whether tpl2 influe ... | 2017 | 28760932 |
| an 18s rrna workflow for characterizing protists in sewage, with a focus on zoonotic trichomonads. | microbial eukaryotes (protists) are important components of terrestrial and aquatic environments, as well as animal and human microbiomes. their relationships with metazoa range from mutualistic to parasitic and zoonotic (i.e., transmissible between humans and animals). despite their ecological importance, our knowledge of protists in urban environments lags behind that of bacteria, largely due to a lack of experimentally validated high-throughput protocols that produce accurate estimates of pro ... | 2017 | 28540488 |
| prevalence of toxoplasma gondii and other gastrointestinal parasites in domestic cats from households in thika region, kenya. | gastrointestinal (git) parasites of domestic cats (felis catus) not only cause morbidity but are also potential zoonotic agents. the current study aimed at establishing the prevalence of git parasites in cats kept by households in thika region, kenya. fecal samples were collected randomly from 103 cats and analyzed for presence of parasites using standard parasitological methods. in descending order, the prevalence of the detected protozoa parasites was isospora spp. 43.7% (95% ci: 40.4-47%), cr ... | 2017 | 28691033 |
| detection of echinococcus multilocularis and other foodborne parasites in fox, cat and dog faeces collected in kitchen gardens in a highly endemic area for alveolar echinococcosis. | echinococcus multilocularis, toxoplasma gondii and toxocara spp. are foodborne parasites whose eggs or oocysts are spread in the environment via canid or felid faeces. they can cause infections in humans following the raw consumption of contaminated fruit or vegetables. in this study, their occurrence was investigated by quantitative polymerase chain reaction (qpcr) in 254 carnivore faeces deposited in 94 kitchen gardens of northeastern france that were sampled between two and six times from oct ... | 2017 | 28748783 |
| occurrence of leishmania infantum in the central nervous system of naturally infected dogs: parasite load, viability, co-infections and histological alterations. | zoonotic visceral leishmaniasis is caused by the protozoan leishmania infantum and little is known about the occurrence and pathogenesis of this parasite in the cns. the aims of this study were to evaluate the occurrence, viability and load of l. infantum in the cns, and to identify the neurological histological alterations associated with this protozoan and its co-infections in naturally infected dogs. forty-eight leishmania-seropositive dogs from which l. infantum was isolated after necropsy w ... | 2017 | 28419136 |
| characterization of sarcocystis fayeri's actin-depolymerizing factor as a toxin that causes diarrhea. | raw horsemeat has the potential to induce food poisoning which often presents with diarrheal symptoms. a sample of horsemeat was found to be infected with sarcocystis fayeri, and a 15-kda protein isolated from the cysts of s. fayeri was found to clearly show its diarrhea-inducing activity. a nested polymerase chain reaction was used to clone the cdna of the 15-kda protein. the deduced amino acid sequence showed homology to actin-depolymerizing factor (adf). a recombinant 15-kda protein depolymer ... | 2017 | 28776895 |
| expression of codon-optimized tgmic16 in three escherichia coli strains. | in a previous study, we found that rabbit anti-toxoplasma gondii serum was capable of recognizing truncated t. gondii microneme protein 16 (tgmic16), indicating that tgmic16 is an essential antigenic t. gondii protein. however, the broad application of this recombinant protein is limited by its low expression level. in this study, we performed codon optimization of tgmic16 by changing the codon-adaptation index from 0.22 to 1.0 without altering the amino acid sequence and expressed the optimized ... | 2017 | 28794925 |
| biochemical characterization of aminopeptidase n2 from toxoplasma gondii. | aminopeptidase n (apn) is a member of the highly conserved m1 family of metalloproteases, and is considered to be a valuable target for the treatment of a variety of diseases, e.g., cancer, malaria, and coccidiosis. in this study, we identified an apn gene (tgapn2) in the toxoplasma gondii genome, and performed a biochemical characterization of the recombinant tgapn2 (rtgapn2) protein. active rtgapn2 was first produced and purified in escherichia coli. the catalytic activity of the enzyme was ve ... | 2017 | 28701624 |
| thioredoxin reductase from toxoplasma gondii: an essential virulence effector with antioxidant function. | thioredoxin reductase (tr) can help pathogens resist oxidative-burst injury from host immune cells by maintaining a thioredoxin-reduction state during nadph consumption. tr is a necessary virulence factor that enables the persistent infection of some parasites. we performed bioinformatics analyses and biochemical assays to characterize the activity, subcellular localization, and genetic ablation of toxoplasma gondii tr (tgtr), to shed light on its biologic function. we expressed the tgtr protein ... | 2017 | 28687608 |
| evaluation of novel oocyst wall protein candidates of toxoplasma gondii. | oocyst stage of toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. thus, it is considered the key for transmission of t. gondii. analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel t. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. five candidates of novel t. gondii oocyst wall proteins ... | 2017 | 28571766 |
| eutirucallin: a lectin with antitumor and antimicrobial properties. | eutirucallin is a lectin isolated from the latex of euphorbia tirucalli, a plant known for its medical properties. the present study explores various characteristics of eutirucallin including stability, cytotoxicity against tumor cells, antimicrobial and antiparasitic activities. eutirucallin was stable from 2 to 40 days at 4°c, maintained hemagglutinating activity within a restricted range, and showed optimal activity at ph 7.0-8.0. eutirucallin presented antiproliferative activity for hela, pc ... | 2017 | 28487845 |
| distal polyneuropathy in an adult birman cat with toxoplasmosis. | a 6-year-old female spayed birman cat presented with a history of weight loss, stiff and short-strided gait in the pelvic limbs and reluctance to jump, progressing to non-ambulatory tetraparesis over 6 weeks. poor body condition, dehydration and generalised muscle wastage were evident on general examination. neurological examination revealed mildly depressed mental status, non-ambulatory flaccid tetraparesis and severely decreased proprioception and spinal reflexes in all four limbs. the neuroan ... | 2017 | 28491412 |
| fatal toxoplasmosis in an immunosuppressed domestic cat from brazil caused by toxoplasma gondii clonal type i. | the objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (felv). a domestic cat (felis silvestris catus) presented intense dyspnea and died three days later. in the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. the histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associate ... | 2017 | 28746448 |
| disease surveillance of california ground squirrels ( spermophilus beecheyi ) in a drive-through zoo in oregon, usa. | rodents and other small wild mammals are often considered to be pests and vectors for disease in zoos that house small populations of valuable threatened and endangered animals. in 2005, three nonhuman primates at a drive-through zoo in oregon, us, acquired tularemia from an unknown source. due to an abundance of california ground squirrels ( spermophilus beecheyi ) on zoo grounds, we instituted serosurveillance of this species from july through september 2008 to determine the prevalence of anti ... | 2017 | 28418764 |
| human infectious diseases and risk of preeclampsia: an updated review of the literature. | preeclampsia (pe) is one of the major causes of maternal and perinatal morbidity and mortality, especially in low- and middle-income countries. in recent years, a growing body of literatures suggests that infections by bacteria, viruses, and parasites and their related inflammations play an important role in the pathogenesis of pe. | 2017 | 28577241 |
| toxoplasma co-infection prevents th2 differentiation and leads to a helminth-specific th1 response. | nematode infections, in particular gastrointestinal nematodes, are widespread and co-infections with other parasites and pathogens are frequently encountered in humans and animals. to decipher the immunological effects of a widespread protozoan infection on the anti-helminth immune response we studied a co-infection with the enteric nematode heligmosomoides polygyrus in mice previously infected with toxoplasma gondii. protective immune responses against nematodes are dependent on parasite-specif ... | 2017 | 28791259 |
| prevalence of pregnancy-relevant infections in a rural setting of ghana. | although infectious diseases still account for a high burden of morbidity and mortality in sub-saharan africa, simultaneous investigations on multiple infections affecting maternal and child health are missing. | 2017 | 28583150 |
| major immunodominant region of hepatitis b virus core antigen as a delivery vector to improve the immunogenicity of the fusion antigen rop2-sag1 multiepitope from toxoplasma gondii in mice. | to prepare the dominant multiepitope fusion antigen rop2-sag1 (rsmultiepitope) from toxoplasma gondii in a prokaryotic system, the major immunodominant region (mir) of the human hepatitis b virus core antigen (hbcag(mir)) was used as a delivery vector. the gene encoding the rsmultiepitope was inserted into hbcag(mir), and rhbcag(mir)-rsmultiepitope was prepared, purified, and administered to balb/c mice through intradermal injection. an indirect enzyme-linked immunosorbent assay analysis based o ... | 2017 | 28436740 |
| assessment of the implementation of the infectious diseases screening programmes among pregnant women in the lesser poland region and comparison with similar programmes conducted in other european union countries. | the aim of the study was to assess the level of implementation of recommendations of the ministry of health regarding infectious disease screening during pregnancy. | 2017 | 28397205 |
| association of cognitive function and liability to addiction with childhood herpesvirus infections: a prospective cohort study. | liability to substance use disorder (sud) is largely nonspecific to particular drugs and is related to behavior dysregulation, including reduced cognitive control. recent data suggest that cognitive mechanisms may be influenced by exposure to neurotropic infections, such as human herpesviruses. in this study, serological evidence of exposure to human herpesvirus herpes simplex virus type 1 (hsv-1), cytomegalovirus (cmv), and epstein-barr virus (ebv) as well as toxoplasma gondii was determined in ... | 2017 | 28420448 |
| pcr-based approach for qualitative molecular analysis of six neurotropic pathogens. | in the last few years, polymerase chain reaction analysis is frequently required to improve the detection of pathogen infections in central nervous system as a potential cause of neurological disorders and neuropsychiatric symptoms. the goal of this paper is to set up a fast, cheap and reliable molecular approach for qualitative detection of six neurotropic pathogens. a method based on pcr has been designed and implemented to guarantee the qualitative dna detection of herpes simplex virus types ... | 2017 | 28854791 |
| microbial vertical transmission during human pregnancy. | congenital infections with pathogens such as zika virus, toxoplasma gondii, listeria monocytogenes, treponema pallidium, parvovirus, hiv, varicella zoster virus, rubella, cytomegalovirus, and herpesviruses are a major cause of morbidity and mortality worldwide. despite the devastating impact of microbial infections on the developing fetus, relatively little is known about how pathogens associated with congenital disease breach the placental barrier to transit vertically during human pregnancy. i ... | 2017 | 28494237 |
| molecular diagnosis of central nervous system opportunistic infections and mortality in hiv-infected adults in central china. | csf pcr is the standard diagnostic technique used in resource-rich settings to detect pathogens of the cns infection. however, it is not currently used for routine csf testing in china. knowledge of cns opportunistic infections among people living with hiv in china is limited. | 2017 | 28469695 |
| non-malarial infectious diseases of antenatal care in pregnant women in franceville, gabon. | in sub-tropical countries, infectious diseases remain one of the main causes of mortality. because of their lack of active immunity, pregnant women and their unborn children represent the most susceptible people. in gabon, data on infectious diseases of pregnant women such as syphilis and rubella are either scarce or very old. few studies have assessed t. gondii infection during pregnancy in the country. here, we evaluate seroprevalence of hiv, htvl-1, syphilis and t. gondii and rubella infectio ... | 2017 | 28606185 |
| structures and kinetics for plant nucleoside triphosphate diphosphohydrolases support a domain motion catalytic mechanism. | extracellular nucleoside triphosphate diphosphohydrolases (ntpdases) are enzymes that hydrolyze extracellular nucleotides to the respective monophosphate nucleotides. in the past 20 years, ntpdases belonging to mammalian, parasitic and prokaryotic domains of life have been discovered, cloned and characterized. we reveal the first structures of ntpdases from the legume plant species trifolium repens (7wc) and vigna unguiculata subsp. cylindrica (dblnp). four crystal structures of 7wc and dblnp we ... | 2017 | 28543850 |
| the er phagosome connection in the era of membrane contact sites. | phagocytosis is an essential mechanism through which innate immune cells ingest foreign material that is either destroyed or used to generate and present antigens and initiate adaptive immune responses. while a role for the er during phagosome biogenesis has been recognized, whether fusion with er cisternae or vesicular derivatives occurs has been the source of much contention. membrane contact sites (mcs) are tight appositions between er membranes and various organelles that coordinate multiple ... | 2017 | 28432021 |
| purinergic signaling: a common path in the macrophage response against mycobacterium tuberculosis and toxoplasma gondii. | immune responses are essential for the protection of the host against external dangers or infections and are normally efficient in the clearance of invading microbes. however, some intracellular pathogens have developed strategies to replicate and survive within host cells resulting in latent infection associated with strong inflammation. this excessive response can cause cell and tissue damage and lead to the release of the intracellular content, in particular the nucleotide pool, into the extr ... | 2017 | 28824882 |
| a rare case of acute toxoplasmosis in a stray dog due to infection of t. gondii clonal type i: public health concern in urban settings with stray animals? | typing of toxoplasma gondii strains is important in epidemiological surveys, to understand the distribution and virulence of different clones of the parasite among human and animal populations. stray dogs can be consider sentinel animals for contaminated environments playing an important but probably under- evaluated role in the epidemiology of t. gondii. we reported a rare case of acute toxoplasmosis in a stray dog due to clonal type i infection. the clonal type i, sporadic in europe, is freque ... | 2017 | 28818085 |
| toxoplasma gondii and neospora caninum induce different host cell responses at proteome-wide phosphorylation events; a step forward for uncovering the biological differences between these closely related parasites. | toxoplasma gondii and neospora caninum are closely related intracellular protozoan parasites and tissue cyst-forming coccidia of the phylum apicomplexa. there are remarkable similarities between the morphology, genomes and transcriptomes of both parasites. toxoplasma is zoonotic, with a wide host range and is mainly transmitted horizontally between its definitive host, the cat, and its intermediate hosts. neospora causes disease within a narrow host range and with reduced virulence potential to ... | 2017 | 28803361 |
| enrofloxacin and toltrazuril are able to reduce toxoplasma gondii growth in human bewo trophoblastic cells and villous explants from human third trimester pregnancy. | classical treatment for congenital toxoplasmosis is based on combination of sulfadiazine and pyrimethamine plus folinic acid. due to teratogenic effects and bone marrow suppression caused by pyrimethamine, the establishment of new therapeutic strategies is indispensable to minimize the side effects and improve the control of infection. previous studies demonstrated that enrofloxacin and toltrazuril reduced the incidence of neospora caninum and toxoplasma gondii infection. the aim of the present ... | 2017 | 28798905 |
| serological survey of neospora caninum and toxoplasma gondii in cattle (bos indicus) and water buffaloes (bubalus bubalis) in ten provinces of brazil. | the objective of this study was to determine the prevalence of antibodies to neospora caninum and toxoplasma gondii among 500 cattle (bos indicus) and 500 buffaloes (bubalus bubalis) using the indirect fluorescent antibody test (ifat) technique. blood samples from were collected from water buffalo and cattle in 10 municipalities in the northern region of brazil. the frequency of cattle and water buffaloes seropositive for neospora caninum in pará state, brazil, was 55% and 44%, respectively, and ... | 2017 | 28673459 |
| new molecular tools in neospora caninum for studying apicomplexan parasite proteins. | the development of molecular genetics has greatly enhanced the study of the biology and pathology associated with parasites of the phylum apicomplexa. we have established a system specifically designed for neospora caninum, and used this system as a heterologous platform for the expression of foreign genes. plasmid constructs containing fluorescent proteins or targeted genes of toxoplasma gondii, driven by n. caninum promoters, have yielded robust expression and correct trafficking of target gen ... | 2017 | 28630403 |
| peroxisome proliferator-activated receptor-γ-mediated polarization of macrophages in neospora caninum infection. | neospora caninum is an apicomplexan parasite closely related toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. macrophage polarization plays an important role in host immune responses to parasites infection, such as toxoplasma gondii, leishmania, trypanosoma cruzi. however, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during n. caninum infection remains unclear. | 2017 | 28527905 |
| seroprevalence of neospora caninum and toxoplasma gondii in exotic ruminants and camelids in the czech republic. | neospora caninum and toxoplasma gondii are the protozoan parasites with definitive hosts from order carnivora. due to vertical transmission, both parasites can cause abortions and neonatal mortality that lead to significant productive and economic losses in the domestic ruminants. the aim of this study was to describe n. caninum and t. gondii seroprevalence in the group of frequently farmed captive exotic ruminants (n = 184) including bovidae (barbary sheep, bezoar goat, common eland, american b ... | 2017 | 28497227 |
| the coccidian parasites toxoplasma and neospora dysregulate mammalian lipid droplet biogenesis. | upon infection, the intracellular parasite toxoplasma gondii co-opts critical functions of its host cell to avoid immune clearance and gain access to nutritional resources. one route by which toxoplasma co-opts its host cell is through hijacking host organelles, many of which have roles in immunomodulation. here we demonstrate that toxoplasma infection results in increased biogenesis of host lipid droplets through rewiring of multiple components of host neutral lipid metabolism. these metabolic ... | 2017 | 28487365 |
| toxoplasma gondii seroprevalence and association with equine protozoal myeloencephalitis: a case-control study of californian horses. | while toxoplasmosis is not commonly considered a clinical disease of equines, previous seroprevalence studies have reported differing background rates of toxoplasma gondii infection in horses globally. the objective of this study was to evaluate possible associations between t. gondii seroprevalence and clinical signs of equine protozoal myeloencephalitis (epm) in horses. using a case-control study design, 720 californian horses with neurologic signs compatible with epm were compared to healthy, ... | 2017 | 28697873 |
| small molecule inhibition of apicomplexan ftsh1 disrupts plastid biogenesis in human pathogens. | the malaria parasite plasmodium falciparum and related apicomplexan pathogens contain an essential plastid organelle, the apicoplast, which is a key anti-parasitic target. derived from secondary endosymbiosis, the apicoplast depends on novel, but largely cryptic, mechanisms for protein/lipid import and organelle inheritance during parasite replication. these critical biogenesis pathways present untapped opportunities to discover new parasite-specific drug targets. we used an innovative screen to ... | 2017 | 28826494 |
| structural and biochemical characterization of apicomplexan inorganic pyrophosphatases. | inorganic pyrophosphatases (ppase) participate in energy cycling and they are essential for growth and survival of organisms. here we report extensive structural and functional characterization of soluble ppases from the human parasites plasmodium falciparum (pfppase) and toxoplasma gondii (tgppase). our results show that pfppase is a cytosolic enzyme whose gene expression is upregulated during parasite asexual stages. cambialistic pfppase actively hydrolyzes linear short chain polyphosphates li ... | 2017 | 28701714 |
| development of loop-mediated isothermal amplification with plasmodium falciparum unique genes for molecular diagnosis of human malaria. | in order to achieve better outcomes for treatment and in the prophylaxis of malaria, it is imperative to develop a sensitive, specific, and accurate assay for early diagnosis of plasmodium falciparum infection, which is the major cause of malaria. in this study, we aimed to develop a loop-mediated isothermal amplification (lamp) assay with p. falciparum unique genes for sensitive, specific, and accurate detection of p. falciparum infection. the unique genes of p. falciparum were randomly selecte ... | 2017 | 28683669 |
| parasites lacking the micronemal protein mic2 are deficient in surface attachment and host cell egress, but remain virulent in vivo. | background: micronemal proteins of the thrombospondin-related anonymous protein (trap) family are believed to play essential roles during gliding motility and host cell invasion by apicomplexan parasites, and currently represent major vaccine candidates against plasmodium falciparum, the causative agent of malaria. however, recent evidence suggests that they play multiple and different roles than previously assumed. here, we analyse a null mutant for mic2, the trap homolog in toxoplasma gondii. ... | 2017 | 28630943 |
| a glycosylphosphatidylinositol-anchored carbonic anhydrase-related protein of toxoplasma gondii is important for rhoptry biogenesis and virulence. | carbonic anhydrase-related proteins (carps) have previously been described as catalytically inactive proteins closely related to α-carbonic anhydrases (α-cas). these carps are found in animals (both vertebrates and invertebrates) and viruses as either independent proteins or domains of other proteins. we report here the identification of a new carp (tgca_rp) in the unicellular organism toxoplasma gondii that is related to the recently described η-class ca found in plasmodium falciparum. tgca_rp ... | 2017 | 28529974 |
| infectious causes of microcephaly: epidemiology, pathogenesis, diagnosis, and management. | microcephaly is an important sign of neurological malformation and a predictor of future disability. the 2015-16 outbreak of zika virus and congenital zika infection brought the world's attention to links between zika infection and microcephaly. however, zika virus is only one of the infectious causes of microcephaly and, although the contexts in which they occur vary greatly, all are of concern. in this review, we summarise important aspects of major congenital infections that can cause microce ... | 2017 | 28844634 |
| seroprevalence of toxoplasma gondii, rubella virus and cytomegalovirus among pregnant women and the importance of avidity assays. | to determine the seroprevalence of toxoplasma gondii (t. gondii), rubella virus, and cytomegalovirus (cmv) among pregnant women in izmir, turkey. | 2017 | 28674718 |
| preliminary multiplex microarray igg immunoassay for the diagnosis of toxoplasmosis and rubella. | during pregnancy, toxoplasmosis and rubella can cause serious damage to the mother and the foetus through vertical transmission. early diagnosis enables implementation of health measures aimed at preventing vertical transmission and minimising damage caused by these diseases. | 2017 | 28591403 |
| the microbiome activates cd4 t-cell-mediated immunity to compensate for increased intestinal permeability. | despite a prominent association, chronic intestinal barrier loss is insufficient to induce disease in human subjects or experimental animals. we hypothesized that compensatory mucosal immune activation might protect individuals with increased intestinal permeability from disease. we used a model in which intestinal barrier loss is triggered by intestinal epithelial-specific expression of constitutively active myosin light chain kinase (ca-mlck). here we asked whether constitutive tight junction ... | 2017 | 28795125 |
| toxoplasma gondii requires glycogen phosphorylase for balancing amylopectin storage and for efficient production of brain cysts. | in immunocompromised hosts, latent infection with toxoplasma gondii can reactivate from tissue cysts, leading to encephalitis. a characteristic of t. gondii bradyzoites in tissue cysts is the presence of amylopectin granules. the regulatory mechanisms and role of amylopectin accumulation in this organism are not fully understood. the t. gondii genome encodes a putative glycogen phosphorylase (tggp), and mutants were constructed to manipulate the activity of tggp and to evaluate the function of t ... | 2017 | 28851850 |
| mrna pseudouridylation affects rna metabolism in the parasite toxoplasma gondii. | rna contains over 100 modified nucleotides that are created post-transcriptionally, among which pseudouridine (ψ) is one of the most abundant. although it was one of the first modifications discovered, the biological role of this modification is still not fully understood. recently, we reported that a pseudouridine synthase (tgpus1) is necessary for differentiation of the single-celled eukaryotic parasite toxoplasma gondii from active to chronic infection. to better understand the biological rol ... | 2017 | 28851751 |
| diagnosis of congenital toxoplasmosis in a renal transplant recipient mother. | we report the case of a first trimester toxoplasmosis infection in a renal transplant recipient. real-time polymerase chain reaction in amniotic fluid at 18 weeks was negative for toxoplasma gondii but at 26 weeks major fetal hydrocephalus was discovered leading to medical termination of pregnancy. pathological examination confirmed lesions consistent with congenital toxoplasmosis. the herein case report as well as data from the french reference centre for congenital toxoplamosis (1835 cases in ... | 2017 | 28851620 |
| rare case of acute toxoplasmosis in a domestic rabbit (oryctolagus cuniculus) in brazil associated with the type briii brazilian clonal lineage of toxoplasma gondii. | toxoplasmosis is a widely distributed disease that infects birds and mammals, including humans. acute clinical course of toxoplasmosis is considered to be rare among domestic rabbits (oryctolagus cuniculus). the aim of this study was to present the first report of fatal acute disease caused by toxoplasma gondii type briii genotype, a typical brazilian clonal lineage, in a domestic rabbit. t. gondii was identified in histological sections of spleen and liver tissue, and these tissues were also im ... | 2017 | 28849263 |
| isolation and characterization of toxoplasma gondii from small ruminants (sheep and goats) in chennai city, south india. | the present study aimed for the isolation and genotyping of toxoplasma gondii from small ruminants (sheep and goats). 14 out of 193 tissue samples (either brain and heart) tested positive by mdat for anti-t. gondii antibodies, were selected and bioassayed, which resulted 4 samples positive for t. gondii after 40 days of post inoculation. four samples consisting of 3 numbers of sheep and 1 number of goat tissues out of 14 samples detected by b1 pcr, were genotyped at sag3 locus by nested polymera ... | 2017 | 28848294 |
| prevalence of toxoplasma gondii infection in household and feral cats in korea. | this study was designed to investigate the prevalence rate of toxoplasma gondii (t. gondii) infection in household cats in korea. one hundred household cats and 50 feral cats from nine of the largest cities in korea were enrolled in this study. the tests performed in this survey was an in-house rapid screen igg and igm combo test, faecal pcr test for t. gondii oocysts, and an elisa immunoassay for igg antibodies. there were no household cats positive for t. gondii infection detected using the in ... | 2017 | 28848285 |
| efficient invasion by toxoplasma depends on the subversion of host protein networks. | apicomplexan parasites are important pathogens of humans and domestic animals, including plasmodium species (the agents of malaria) and toxoplasma gondii, which is responsible for toxoplasmosis. they replicate within the cells of their animal hosts, to which they gain access using a unique parasite-driven invasion process. at the core of the invasion machine is a structure at the interface between the invading parasite and host cell called the moving junction (mj) (1) . the mj serves as both a m ... | 2017 | 28848228 |
| the lymphotoxin β receptor is essential for upregulation of ifn-induced guanylate-binding proteins and survival after toxoplasma gondii infection. | lymphotoxin β receptor (ltβr) signaling plays an important role in efficient initiation of host responses to a variety of pathogens, encompassing viruses, bacteria, and protozoans via induction of the type i interferon response. the present study reveals that after toxoplasma gondii infection, ltβr(-/-) mice show a substantially reduced survival rate when compared to wild-type mice. ltβr(-/-) mice exhibit an increased parasite load and a more pronounced organ pathology. also, a delayed increase ... | 2017 | 28845089 |
| first genetic characterization of toxoplasma gondii infection in poultry meat intended for human consumption in eastern china. | toxoplasma gondii can infect nearly all warm-blooded animals including birds. however, limited information on the molecular epidemiology and genotypes of t. gondii infecting poultry is available in china. therefore, the present study characterized t. gondii genotypes in poultry meat in eastern china. during august 2015 and september 2016, muscle tissue samples collecting from 414 poultries (257 chickens, 115 ducks and 42 geese) in shandong provinces were used to detect the t. gondii b1 gene by a ... | 2017 | 28842355 |
| the effect of lopinavir/ritonavir and lopinavir/ritonavir loaded plga nanoparticles on experimental toxoplasmosis. | a marked reduction has been achieved in the incidence and clinical course of toxoplasmic encephalitis after the introduction of protease inhibitors within the treatment regimen of hiv (hiv-pis). this work was undertaken to study for the first time, the efficacy of hiv-pis, lopinavir/ritonavir (l/r), as a therapeutic agent in acute experimental toxoplasmosis. lopinavir/ritonavir (l/r) were used in the same ratio present in aluvia, a known hiv-pis drug used in the developing countries in the treat ... | 2017 | 28838776 |
| a rare case of pulmonary toxoplasmosis in a patient with undifferentiated inflammatory arthritis on chronic methotrexate and corticosteroid therapy. | pulmonary toxoplasmosis is a serious pulmonary condition caused by the protozoan toxoplasma gondii it typically affects immunocompromised patients presenting acutely with cough, fever, myalgias, arthralgias and lymphadenopathy, and chronically with persistent cough and dyspnoea. because of its protean features, it can mimic many more common lung conditions in the immunocompromised patient, including atypical pneumonia, pneumocystis pneumonia and interstitial lung disease. in this article, we pre ... | 2017 | 28835429 |
| the toxoplasma centrocone houses cell cycle regulatory factors. | our knowledge of cell cycle regulatory mechanisms in apicomplexan parasites is very limited. in this study, we describe a novel toxoplasma gondii factor that has a vital role in chromosome replication and the regulation of cytoplasmic and nuclear mitotic structures, and we named this factor ecr1 for essential for chromosome replication 1. ecr1 was discovered by complementation of a temperature-sensitive (ts) mutant that suffers lethal, uncontrolled chromosome replication at 40°c similar to a ts ... | 2017 | 28830940 |
| development of direct assays for toxoplasma gondii and its use in genomic dna sample. | this work describes an approach for the selection and detection of specific dna probes related to toxoplasma gondii, a protozoan parasite responsible for toxoplasmosis. the detection system was developed on graphite carbon electrode modified with poly(3-hydroxybenzoic acid) sensitized with toxg1 probe. the hybridization of the specific genomic dna related to t. gondii showed good response by direct detection of guanine residue oxidation using differential pulse voltammetry (dpv). the biosensor w ... | 2017 | 28826142 |
| lack of serological and molecular association between toxoplasma gondii exposure and obesity: a case-control study. | the association between t. gondii infection and obesity has been scantly studied. through an age-, and gender-matched case-control study, we determined the association of t. gondii infection and obesity using serological and molecular methods. cases included 203 persons with obesity, and controls included 203 persons without obesity. participants were tested for the presence of anti-toxoplasma igg antibodies using an enzyme-linked immunoassay (eia). igg seropositive individuals were further test ... | 2017 | 28824343 |
| dissecting amyloid beta deposition using distinct strains of the neurotropic parasite toxoplasma gondii as a novel tool. | genetic and pathologic data suggest that amyloid beta (aβ), produced by processing of the amyloid precursor protein, is a major initiator of alzheimer's disease (ad). to gain new insights into aβ modulation, we sought to harness the power of the coevolution between the neurotropic parasite toxoplasma gondii and the mammalian brain. two prior studies attributed toxoplasma-associated protection against aβ to increases in anti-inflammatory cytokines (tgf-β and il-10) and infiltrating phagocytic mon ... | 2017 | 28817954 |
| diphenyl diselenide modulates nucleotidases, reducing inflammatory responses in the liver of toxoplasma gondii-infected mice. | the aim of this study was to verify the effect of diphenyl diselenide (phse)2 on hepatic nucleotidases and on the concentration of purines in mice infected by toxoplasma gondii. the animals were divided into four groups: group a (uninfected), group b (uninfected and treated with (phse)2), group c (infected), and group d (infected and treated with (phse)2). the inoculation (groups c and d) was performed with 50 cysts of t. gondii (me-49 strain). mice from groups b and d were treated with 5 μmol k ... | 2017 | 28815408 |
| concordance of toxoplasma gondii infection markers among heterosexual couples. | very little is known about concordance of toxoplasma gondii (t. gondii) infection markers among couples. through a cross-sectional study, we sought to determine the correlation of t. gondii infection in a sample of 119 heterosexual couples in durango state, mexico. | 2017 | 28811857 |
| cryo-em structures of the 80s ribosomes from human parasites trichomonas vaginalis and toxoplasma gondii. | as an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. high-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. with cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, trichomonas vaginalis ... | 2017 | 28809395 |
| prevalence and genetic characterization of toxoplasma gondii in donkeys in northeastern china. | toxolasma gondii is one of the most important obligate intracellular protozoan parasites. recently, toxoplasmosis is of increasing concerns because t. gondii not only has a worldwide distribution but also can infect virtually all warm-blooded hosts including donkeys. however, limited information is available concerning the genetic characterization of t. gondii in donkeys in northeastern china. in this study, a total of 302 brain tissue samples collected from donkeys from jilin (n=108) and liaoni ... | 2017 | 28807857 |
| targeted overexpression of cyclic amp-dependent protein kinase subunit in toxoplasma gondii promotes replication and virulence in host cells. | toxoplasma gondii (t. gondii) is one of the most common parasite that can infect almost any warm-blooded animals including humans. the cyclic nucleotide-dependent protein kinase (pka) regulates a spectrum of intracellular signal pathways in many organisms. protein kinase catalytic subunit (pkac) is the core of the whole protein, and plays an important role in the life cycle of t.gondii. here, t.gondii pkac (tgpkac) overexpression strain (tgpkac-oe) was constructed. the growth of the tgpkac-oe, r ... | 2017 | 28807302 |
| recombinase polymerase amplification (rpa) combined with lateral flow (lf) strip for detection of toxoplasma gondii in the environment. | toxoplasma gondii infects all warm-blooded vertebrates, resulting in a great threat to human health and significant economic loss to the livestock industry. ingestion of infectious oocysts of t. gondii from the environment is the major source of transmission. detection of t. gondii oocysts by existing methods is laborious, time-consuming and expensive. the objective of the present study was to develop a recombinase polymerase amplification (rpa) method combined with a lateral flow (lf) strip for ... | 2017 | 28807294 |
| toxoplasma gondii in the cornigliese sheep breed in italy: meat juice serology, in vitro isolation and genotyping. | toxoplasma gondii is considered one of the most important food-borne parasitic zoonoses globally and sheep are important intermediate hosts of the parasite. meat and milk from infected sheep are considered an important source of infection for humans. here, the authors evaluated t. gondii infection in the italian cornigliese sheep breed using meat juice elisa, and in vitro assay for followed by real time-pcr and pcr-rflp. twenty-one hearts were collected at slaughter. meat juice serology was carr ... | 2017 | 28807281 |
| isolation of viable toxoplasma gondii, molecular characterization, and seroprevalence in elk (cervus canadensis) in pennsylvania, usa. | toxoplasmosis is a worldwide zoonosis. the ingestion of uncooked/undercooked meat and consumption of water contaminated with toxoplasma gondii oocysts excreted by felids are the main modes of transmission of this parasite. t. gondii has been reported in multiple cervid species; however, little is known of the parasite in north american elk (cervus canadensis). in the present study, antibodies to t. gondii were detected in serum of wild elk from pennsylvania collected during 2013-2016 by the modi ... | 2017 | 28807274 |
| azithromycin treatment is able to control the infection by two genotypes of toxoplasma gondii in human trophoblast bewo cells. | trophoblast infection by toxoplasma gondii plays a pivotal role in the vertical transmission of toxoplasmosis. here, we investigate whether the antibiotic therapy with azithromycin, spiramycin and sulfadiazine/pyrimethamine are effective to control trophoblast infection by two brazilian t. gondii genotypes, tgchbrud1 or tgchbrud2. two antibiotic protocols were evaluated, as follow: i) pre-treatment of t. gondii-tachyzoites with selected antibiotics prior trophoblast infection and ii) post-treatm ... | 2017 | 28803905 |
| utility of the cytochrome c oxidase subunit i gene for the diagnosis of toxoplasmosis using pcr. | toxoplasmosis is caused by the protozoan parasite toxoplasma gondii, which belongs to the phylum apicomplexa. since this parasite causes severe clinical symptoms in immunocompromised patients, early diagnosis of toxoplasmosis is essential. pcr is currently used for early diagnosis, but there is no consensus regarding the most effective method for amplifying toxoplasma dna. in this study, we considered the utility of the cytochrome c subunit i (cox1) gene, which is encoded in the mitochondrial dn ... | 2017 | 28803790 |