Publications

detection of human anti-zika virus igg by elisa using an antigen from in vitro infected vero cells: preliminary results.	zika virus (zkv) infection is a huge public health problem in brazil because of the increased incidence of microcephaly in neonates from infected mothers. detection of specific igg antibodies in maternal serum samples constitutes an important approach for diagnosing zkv infection and evaluating its relationship with neonatal microcephaly. however, as there is no serological test produced in brazil to detect igm and igg antibodies against zkv, we sought to examine specific igg in serum samples fr ...	2016	27982355
invasive mutualisms between a plant pathogen and insect vectors in the middle east and brazil.	complex multi-trophic interactions in vectorborne diseases limit our understanding and ability to predict outbreaks. arthropod-vectored pathogens are especially problematic, with the potential for novel interspecific interactions during invasions. variations and novelties in plant-arthropod-pathogen triumvirates present significant threats to global food security. we examined aspects of a phytoplasma pathogen of citrus across two continents. 'candidatus phytoplasma aurantifolia' causes witches' ...	2016	28083099
inhibition and larvicidal activity of phenylpropanoids from piper sarmentosum on acetylcholinesterase against mosquito vectors and their binding mode of interaction.	aedes aegypti, aedes albopictus and culex quinquefasciatus are vectors of dengue fever and west nile virus diseases. this study was conducted to determine the toxicity, mechanism of action and the binding interaction of three active phenylpropanoids from piper sarmentosum (piperaceae) toward late 3rd or early 4th larvae of above vectors. a bioassay guided-fractionation on the hexane extract from the roots of piper sarmentosum led to the isolation and identification of three active phenylpropanoi ...	2016	27152416
zika virus: a new animal model for an arbovirus.		2016	27149628
chikungunya fever cases identified in the veterans health administration system, 2014.	during december 2013, the first locally transmitted chikungunya virus (chikv) infections in the americas were reported in the caribbean. although chikv infection is rarely fatal, risk for severe disease increases with age and medical comorbidities. herein we describe characteristics of veterans health administration (vha) patients with chikv infection and, among those with infections diagnosed in puerto rico, investigated risk factors for hospitalization.	2016	27144588
multi-stakeholder decision aid for improved prioritization of the public health impact of climate sensitive infectious diseases.	the effects of climate change on infectious diseases are an important global health concern and necessitate decisions for allocation of resources. economic tools have been used previously; however, how prioritization results might differ when done using broader considerations identified by local stakeholders has yet to be assessed. a multicriteria decision analysis (mcda) approach was used to assess multi-stakeholder expressed concerns around disease prioritization via focus groups held in quebe ...	2016	27077875
zika virus outbreak in rio de janeiro, brazil: clinical characterization, epidemiological and virological aspects.	in 2015, brazil was faced with the cocirculation of three arboviruses of major public health importance. the emergence of zika virus (zikv) presents new challenges to both clinicians and public health authorities. overlapping clinical features between diseases caused by zikv, dengue (denv) and chikungunya (chikv) and the lack of validated serological assays for zikv make accurate diagnosis difficult.	2016	27070912
flavivirus vaccines: innovate, invest, implement.		2016	27211530
harnessing the power of defensive microbes: evolutionary implications in nature and disease control.		2016	27058881
neglected tropical diseases in the anthropocene: the cases of zika, ebola, and other infections.		2016	27058728
digestion of yeasts and beta-1,3-glucanases in mosquito larvae: physiological and biochemical considerations.	aedes aegypti larvae ingest several kinds of microorganisms. in spite of studies regarding mosquito digestion, little is known about the nutritional utilization of ingested cells by larvae. we investigated the effects of using yeasts as the sole nutrient source for a. aegypti larvae. we also assessed the role of beta-1,3-glucanases in digestion of live yeast cells. beta-1,3-glucanases are enzymes which hydrolyze the cell wall beta-1,3-glucan polyssacharide. larvae were fed with cat food (control ...	2016	27007411
navigating the zika panic.	the epidemics of ebola virus in west africa and zika virus in america highlight how viruses can explosively emerge into new territories. these epidemics also exposed how unprepared we are to handle infectious disease emergencies. this is also true when we consider hypothesized new clinical features of infection, such as the associations between zika virus infection and severe neurological disease, including microcephaly and guillain-barré syndrome. on the surface, these pathologies appear to be ...	2016	27746903
preventing zika virus infection during pregnancy using a seasonal window of opportunity for conception.	it has come to light that zika virus (zikv) infection during pregnancy can result in trans-placental transmission to the fetus along with fetal death, congenital microcephaly, and/or central nervous system (cns) malformations. there are projected to be >9,200,000 births annually in countries with ongoing zikv transmission. in response to the zikv threat, the world health organization (who) is strategically targeting prevention of infection in pregnant women and funding contraception in epidemic ...	2016	27467271
selective factors associated with the evolution of codon usage in natural populations of arboviruses.	arboviruses (arthropod borne viruses) have life cycles that include both vertebrate and invertebrate hosts with substantial differences in vector and host specificity between different viruses. most arboviruses utilize rna for their genetic material and are completely dependent on host trnas for their translation, suggesting that virus codon usage could be a target for selection. in the current study we analyzed the relative synonymous codon usage (rscu) patterns of 26 arboviruses together with ...	2016	27455096
infection rates by dengue virus in mosquitoes and the influence of temperature may be related to different endemicity patterns in three colombian cities.	colombia is an endemic country for dengue fever where the four serotypes of virus dengue (denv1-4) circulate simultaneously, and all types are responsible for dengue cases in the country. the control strategies are guided by entomological surveillance. however, heterogeneity in aedic indices is not well correlated with the incidence of the disease in cities such as riohacha, bello and villavicencio. as an alternative, molecular detection of dengue virus in mosquitoes has been proposed as a usefu ...	2016	27455289
composition and genetic diversity of mosquitoes (diptera: culicidae) on islands and mainland shores of kenya's lakes victoria and baringo.	the lake baringo and lake victoria regions of kenya are associated with high seroprevalence of mosquito-transmitted arboviruses. however, molecular identification of potential mosquito vector species, including morphologically identified ones, remains scarce. to estimate the diversity, abundance, and distribution of mosquito vectors on the mainland shores and adjacent inhabited islands in these regions, we collected and morphologically identified adult and immature mosquitoes and obtained the co ...	2016	27402888
using stable isotopes of carbon and nitrogen to mark wild populations of anopheles and aedes mosquitoes in south-eastern tanzania.	marking wild mosquitoes is important for understanding their ecology, behaviours and role in disease transmission. traditional insect marking techniques include using fluorescent dyes, protein labels, radioactive labels and tags, but such techniques have various limitations; notably low marker retention and inability to mark wild mosquitoes at source. stable isotopes are gaining wide spread use for non-invasive marking of arthropods, permitting greater understanding of mosquito dispersal and res ...	2016	27392083
vector competence of northern european culex pipiens biotypes and hybrids for west nile virus is differentially affected by temperature.	outbreaks of west nile virus (wnv) have not occurred in northern europe despite nearby circulation of wnv in the southern part of the continent. the main vector for wnv, the mosquito culex (cx.) pipiens, consists of two behaviorally distinct biotypes, pipiens and molestus, which can form hybrids. although temperature has been shown to influence vector competence of cx. pipiens for wnv and biotypes are differentially susceptible towards infection, the interaction between the two has not been eluc ...	2016	27388451
infectivity of immature neurons to zika virus: a link to congenital zika syndrome.	epidemiological data strongly suggest that microcephaly cases in brazil are associated with the ongoing epidemic of zika virus (zikv). in order to further solidify the possible link, we investigated the infectivity of zikv using various neuroblastoma (nb) cell lines.	2016	27364784
has rift valley fever virus evolved with increasing severity in human populations in east africa?	rift valley fever (rvf) outbreaks have occurred across eastern africa from 1912 to 2010 approximately every 4-15 years, most of which have not been accompanied by significant epidemics in human populations. however, human epidemics during rvf outbreaks in eastern africa have involved 478 deaths in 1998, 1107 reported cases with 350 deaths from 2006 to 2007 and 1174 cases with 241 deaths in 2008. we review the history of rvf outbreaks in eastern africa to identify the epidemiological factors that ...	2016	27329846
spatial and temporal hot spots of aedes albopictus abundance inside and outside a south european metropolitan area.	aedes albopictus is a tropical invasive species which in the last decades spread worldwide, also colonizing temperate regions of europe and us, where it has become a public health concern due to its ability to transmit exotic arboviruses, as well as severe nuisance problems due to its aggressive daytime outdoor biting behaviour. while several studies have been carried out in order to predict the potential limits of the species expansions based on eco-climatic parameters, few studies have so far ...	2016	27333276
establishment of a novel primary human skeletal myoblast cellular model for chikungunya virus infection and pathogenesis.	chikungunya virus (chikv) is a re-emerging arbovirus known to cause chronic myalgia and arthralgia and is now considered endemic in countries across asia and africa. the tissue tropism of chikv infection in humans remains, however, ill-defined. due to the fact that myositis is commonly observed in most patients infected with chikv, we sought to develop a clinically relevant cellular model to better understand the pathogenesis of chikv infection. in this study, primary human skeletal muscle myobl ...	2016	26892458
high prevalence of west nile virus in domestic birds and detection in 2 new mosquito species in madagascar.	west nile virus is an arthropod-borne zoonosis transmitted by a large number of mosquito species, and birds play a key role as reservoir of the virus. its distribution is largely widespread over africa, asia, the americas and europe. since 1978, it has frequently been reported in madagascar. studies described a high seroprevalence level of the virus in humans in different areas of the island and a human fatal case of wnv infection was reported in 2011. despite these reports, the epidemiology of ...	2016	26807720
introduction and transmission of zika virus in brazil: new challenges for the americas.		2016	27007567
comparative genomic analysis of pre-epidemic and epidemic zika virus strains for virological factors potentially associated with the rapidly expanding epidemic.	less than 20 sporadic cases of human zika virus (zikv) infection were reported in africa and asia before 2007, but large outbreaks involving up to 73% of the populations on the pacific islands have started since 2007, and spread to the americas in 2014. moreover, the clinical manifestation of zikv infection has apparently changed, as evident by increasing reports of neurological complications, such as guillain-barré syndrome in adults and congenital anomalies in neonates. we comprehensively comp ...	2016	26980239
outbreak of zika virus disease in the americas and the association with microcephaly, congenital malformations and guillain-barré syndrome.		2016	26998633
human antibody response to dengue virus: implications for dengue vaccine design.	dengue, a global health threat, is a leading cause of morbidity and mortality in most tropical and subtropical countries. dengue can range from asymptomatic, relatively mild dengue fever to severe and life-threatening dengue hemorrhagic fever. disease severity and outcome is largely associated with the host immune response. several candidate vaccines in clinical trials appear promising as effective measures for dengue disease control. vaccine development has been hampered by safety and efficacy ...	2016	27398060
zika virus and pregnancy: a review of the literature and clinical considerations.	the latest zika virus (zikv) outbreak has reached epidemic proportions as it spreads throughout south and central america. in november 2015, the brazilian ministry of health reported a 20-fold increase in the number of cases of neonatal microcephaly, which corresponds geographically and temporally to the zikv outbreak. case reports have provided some evidence of a causal link between maternal zikv infection, fetal microcephaly, and intracranial calcifications. the sparse data regarding zikv in p ...	2016	26939047
the effect of west nile virus infection on the midgut gene expression of culex pipiens quinquefasciatus say (diptera: culicidae).	the interaction of the mosquito and the invading virus is complex and can result in physiological and gene expression alterations in the insect. the association of west nile virus (wnv) and culex pipiens quinquefasciatus mosquitoes results in measurable changes in gene expression; 22 gene products were shown previously to have altered expression. sequence analysis of one product, cq g1a1, revealed 100% amino acid identity to gram negative bacteria binding proteins (cpqgbp) in cx. p. quinquefasci ...	2016	27999244
potential for zika virus to establish a sylvatic transmission cycle in the americas.	zika virus (zikv) originated and continues to circulate in a sylvatic transmission cycle between non-human primate hosts and arboreal mosquitoes in tropical africa. recently zikv invaded the americas, where it poses a threat to human health, especially to pregnant women and their infants. here we examine the risk that zikv will establish a sylvatic cycle in the americas, focusing on brazil. we review the natural history of sylvatic zikv and present a mathematical dynamic transmission model to as ...	2016	27977671
chikungunya virus infection and diabetes mellitus: a double negative impact.	the impact of chikungunya virus (chikv) infection on diabetic patients (dps) has not been described. we aimed to compare clinical features of chikv infection in dps and nondiabetic patients (ndps), and to evaluate its effects on glycemic control among dps. we recorded clinical information and, in dps, glycemic control. forty-six dps and 53 ndps aged ≥ 20 years living in haiti, with acute chikv infection, were studied. diabetes duration was 7.1 ± 6.1 years. the most common acute chikv clinical ma ...	2016	27729569
dengue in the middle east and north africa: a systematic review.	dengue virus (denv) infection is widespread and its disease burden has increased in past decades. however, little is known about the epidemiology of dengue in the middle east and north africa (mena).	2016	27926925
review of west nile virus circulation and outbreak risk in madagascar: entomological and ornithological perspectives.	west nile fever (wnf) is a zoonotic disease, occurring nearly globally. in madagascar, west nile virus (wnv) was first detected in 1978 from wild birds and the virus is currently distributed across the island, but no epidemic or epizootic period has been recorded. one fatal human case of wnv infection was reported in 2011, suggesting a "tip of the iceberg" phenomenon of a possible wnf epidemic/epizootic on the island. the main objective of this literature-based survey is to review patterns of wn ...	2016	27849515
a comprehensive systems biology approach to studying zika virus.	zika virus (zikv) is responsible for an ongoing and intensifying epidemic in the western hemisphere. we examined the complete predicted proteomes, glycomes, and selectomes of 33 zikv strains representing temporally diverse members of the african lineage, the asian lineage, and the current outbreak in the americas. derivation of the complete selectome is an 'omics' approach to identify distinct evolutionary pressures acting on different features of an organism. employment of the m8 model did not ...	2016	27584813
culex pipiens and aedes triseriatus mosquito susceptibility to zika virus.		2016	27434194
research contributing to improvements in controlling florida's mosquitoes and mosquito-borne diseases.	research on mosquitoes and mosquito-borne diseases has contributed to improvements in providing effective, efficient, and environmentally proper mosquito control. florida has benefitted from several research accomplishments that have increased the state's mosquito control capabilities. research with florida's mosquitoes has resulted in the development of ecologically sound management of mosquito impoundments on florida's east coast. this strategy, called rotational impoundment management (rim), ...	2016	27690112
dengueme: a tool for the modeling and simulation of dengue spatiotemporal dynamics.	the prevention and control of dengue are great public health challenges for many countries, particularly since 2015, as other arboviruses have been observed to interact significantly with dengue virus. different approaches and methodologies have been proposed and discussed by the research community. an important tool widely used is modeling and simulation, which help us to understand epidemic dynamics and create scenarios to support planning and decision making processes. with this aim, we propo ...	2016	27649226
aedes aegypti d7 saliva protein inhibits dengue virus infection.	aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (denv) types 1-4. ae. aegypti transmits denv by inoculating virus-infected saliva into host skin during probing and feeding. ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. previously, we showed that ae. aegypti salivary gland extracts (sges) enhanced dissemination of denv to draining lymph nodes. in con ...	2016	27632170
population genetic structure of aedes fluviatilis (diptera: culicidae).	although aedes fluviatilis is an anthropophilic mosquito found abundantly in urban environments, its biology, epidemiological potential and genetic characteristics are poorly understood. climate change and urbanization processes that result in environmental modifications benefit certain anthropophilic mosquito species such as ae. fluviatilis, greatly increasing their abundance in urban areas. to gain a better understanding of whether urbanization processes modulate the genetic structure of this ...	2016	27598889
plant virus-insect vector interactions: current and potential future research directions.	acquisition and transmission by an insect vector is central to the infection cycle of the majority of plant pathogenic viruses. plant viruses can interact with their insect host in a variety of ways including both non-persistent and circulative transmission; in some cases, the latter involves virus replication in cells of the insect host. replicating viruses can also elicit both innate and specific defense responses in the insect host. a consistent feature is that the interaction of the virus wi ...	2016	27834855
downregulation of aedes aegypti chromodomain helicase dna binding protein 7/kismet by wolbachia and its effect on dengue virus replication.	dengue virus (denv) is a mosquito-transmitted virus imposing a significant burden on human health around the world. since current control strategies are not sufficient, there is an urgent need to find alternative methods to control denv transmission. it has been demonstrated that introduction of wolbachia pipientis in aedes aegypti mosquitoes can impede denv transmission with the mechanism(s) not fully understood. recently, a number of studies have found the involvement of chromodomain dna bindi ...	2016	27827425
zika virus epidemic in brazil. i. fatal disease in adults: clinical and laboratorial aspects.	zika virus (zikv) was first detected in brazil in may 2015 and the country experienced an explosive epidemic. however, recent studies indicate that the introduction of zikv occurred in late 2013. cases of microcephaly and deaths associated with zikv infection were identified in brazil in november, 2015.	2016	27835759
transmission bottlenecks and rnai collectively influence tick-borne flavivirus evolution.	arthropod-borne rna viruses exist within hosts as heterogeneous populations of viral variants and, as a result, possess great genetic plasticity. understanding the micro-evolutionary forces shaping these viruses can provide insights into how they emerge, adapt, and persist in new and changing ecological niches. while considerable attention has been directed toward studying the population dynamics of mosquito-borne viruses, little is known about tick-borne virus populations. therefore, using a mo ...	2016	28058113
distribution of the members of the pipiens assemblage in the sympatric area from argentina: which is where and when?	given their medical and veterinary relevance, the members of the pipiens assemblage are a worldwide target of ecological research. the distribution of culex pipiens s.s. and cx. quinquefasciatus converge in buenos aires, argentina, where hybrids have been detected. each member of the assemblage exhibits a distinct eco-physiological behaviour that can affect its efficiency in pathogen transmission. our aim was to identify the environmental drivers for the spatio-temporal distribution of each memb ...	2016	27783720
transstadial transmission and long-term association of crimean-congo hemorrhagic fever virus in ticks shapes genome plasticity.	the trade-off hypothesis, the current paradigm of arbovirus evolution, proposes that cycling between vertebrate and invertebrate hosts presents significant constraints on genetic change of arboviruses. studying these constraints in mosquito-borne viruses has led to a new understanding of epizootics. the trade-off hypothesis is assumed to be applicable to tick-borne viruses too, although studies are lacking. tick-borne crimean-congo hemorrhagic fever virus (cchfv), a member of the family bunyavir ...	2016	27775001
combining attractants and larvicides in biodegradable matrices for sustainable mosquito vector control.	there is a global need for cost-effective and environmentally friendly tools for control of mosquitoes and mosquito-borne diseases. one potential way to achieve this is to combine already available tools to gain synergistic effects to reduce vector mosquito populations. another possible way to improve mosquito control is to extend the active period of a given control agent, enabling less frequent applications and consequently, more efficient and longer lasting vector population suppression.	2016	27768698
looking backward, looking forward: the long, torturous struggle with mosquitoes.	the american anti-mosquito movement grew out of the discovery of the role of mosquitoes in transferring pathogens and public concern about pest and nuisance mosquitoes in the late 1800s. in the 20th century, organized mosquito control in the united states passed through three eras: mechanical, chemical, and integrated mosquito control. mosquito control in the 21st century faces the challenge of emerging pathogens, invasive mosquito species, and balancing concerns about the environment with effec ...	2016	27775554
zika antiviral chemotherapy: identification of drugs and promising starting points for drug discovery from an fda-approved library.	background the recent epidemics of zika virus (zikv) implicated it as the cause of serious and potentially lethal congenital conditions such microcephaly and other central nervous system defects, as well as the development of the guillain-barré syndrome in otherwise healthy patients. recent findings showed that anti-dengue antibodies are capable of amplifying zikv infection by a mechanism similar to antibody-dependent enhancement, increasing the severity of the disease. this scenario becomes pot ...	2016	27909576
how did zika virus emerge in the pacific islands and latin america?	the unexpected emergence of zika virus (zikv) in the pacific islands and latin america and its association with congenital zika virus syndrome (czvs) (which includes microcephaly) and guillain-barré syndrome (gbs) have stimulated wide-ranging research. high densities of susceptible aedes spp., immunologically naive human populations, global population growth with increased urbanization, and escalation of global transportation of humans and commercial goods carrying vectors and zikv undoubtedly e ...	2016	27729507
forecasting united states heartworm dirofilaria immitis prevalence in dogs.	this paper forecasts next year's canine heartworm prevalence in the united states from 16 climate, geographic and societal factors. the forecast's construction and an assessment of its performance are described.	2016	27724981
massive yet grossly underestimated global costs of invasive insects.	insects have presented human society with some of its greatest development challenges by spreading diseases, consuming crops and damaging infrastructure. despite the massive human and financial toll of invasive insects, cost estimates of their impacts remain sporadic, spatially incomplete and of questionable quality. here we compile a comprehensive database of economic costs of invasive insects. taking all reported goods and service estimates, invasive insects cost a minimum of us$70.0 billion p ...	2016	27698460
dna microarray platform for detection and surveillance of viruses transmitted by small mammals and arthropods.	viruses transmitted by small mammals and arthropods serve as global threats to humans. most emergent and re-emergent viral agents are transmitted by these groups; therefore, the development of high-throughput screening methods for the detection and surveillance of such viruses is of great interest. in this study, we describe a dna microarray platform that can be used for screening all viruses transmitted by small mammals and arthropods (smaviruschip) with nucleotide sequences that have been depo ...	2016	27654806
detection of invasive mosquito vectors using environmental dna (edna) from water samples.	repeated introductions and spread of invasive mosquito species (ims) have been recorded on a large scale these last decades worldwide. in this context, members of the mosquito genus aedes can present serious risks to public health as they have or may develop vector competence for various viral diseases. while the tiger mosquito (aedes albopictus) is a well-known vector for e.g. dengue and chikungunya viruses, the asian bush mosquito (ae. j. japonicus) and ae. koreicus have shown vector competenc ...	2016	27626642
new paradigms for virus detection, surveillance and control of zika virus vectors in the settings of southeast asia.	zika virus (zikv) has now become a global public health concern. the vectors for zikv are aedes aegypti and a. albopictus. both these mosquitoes are predominant in southeast asia and are also responsible for the spread of other arboviral diseases like dengue virus and chikungunya virus. the incidence of dengue has been increasing over the years and this is of concern to public health workers. simple laboratory tools for the detection of zikv is also lacking. in the absence of drugs and vaccine f ...	2016	27679623
nonstructural proteins are preferential positive selection targets in zika virus and related flaviviruses.	the flavivirus genus comprises several human pathogens such as dengue virus (denv), japanese encephalitis virus (jev), and zika virus (zikv). although zikv usually causes mild symptoms, growing evidence is linking it to congenital birth defects and to increased risk of guillain-barré syndrome. zikv encodes a polyprotein that is processed to produce three structural and seven nonstructural (ns) proteins. we investigated the evolution of the viral polyprotein in zikv and in related flaviviruses (d ...	2016	27588756
molecular signatures associated with zikv exposure in human cortical neural progenitors.	zika virus (zikv) infection causes microcephaly and has been linked to other brain abnormalities. how zikv impairs brain development and function is unclear. here we systematically profiled transcriptomes of human neural progenitor cells exposed to asian zikv(c), african zikv(m), and dengue virus (denv). in contrast to the robust global transcriptome changes induced by denv, zikv has a more selective and larger impact on expression of genes involved in dna replication and repair. while overall e ...	2016	27580721
zika fetal neuropathogenesis: etiology of a viral syndrome.	the ongoing zika virus epidemic in the americas and the observed association with both fetal abnormalities (primary microcephaly) and adult autoimmune pathology (guillain-barré syndrome) has brought attention to this neglected pathogen. while initial case studies generated significant interest in the zika virus outbreak, larger prospective epidemiology and basic virology studies examining the mechanisms of zika viral infection and associated pathophysiology are only now starting to be published. ...	2016	27560129
modeling mosquito-borne disease spread in u.s. urbanized areas: the case of dengue in miami.	expansion of mosquito-borne pathogens into more temperate regions of the world necessitates tools such as mathematical models for understanding the factors that contribute to the introduction and emergence of a disease in populations naïve to the disease. often, these models are not developed and analyzed until after a pathogen is detected in a population. in this study, we develop a spatially explicit stochastic model parameterized with publicly available u.s. census data for studying the poten ...	2016	27532496
a community-based prospective cohort study of dengue viral infection in malaysia: the study protocol.	globally, dengue infections constitute a significant public health burden. in recent decades, malaysia has become a dengue hyper-endemic country with the co-circulation of the four dengue virus serotypes. the cyclical dominance of sub-types contributes to a pattern of major outbreaks. the consequences can be observed in the rising incidence of reported dengue cases and dengue related deaths. understanding the complex interaction of the dengue virus, its human hosts and the mosquito vectors at th ...	2016	27510731
development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease.	dengue disease is one of the most significant vector-borne illnesses in the world. the emergence and re-emergence of dengue infections in many parts of the world affect millions annually and continue to burden public health systems especially in low-income populations. advances in dengue vaccine development showed promising results; however, protection seems to be suboptimal. there is no licensed chemotherapeutic agent against dengue to date. an ideal scenario of combinatorial vaccination of hig ...	2016	27551237
larval application of sodium channel homologous dsrna restores pyrethroid insecticide susceptibility in a resistant adult mosquito population.	mosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses and other parasitic microorganisms. the emergence and spread of insecticide resistance is threatening the effectiveness of current control measures for common mosquito vector borne diseases, such as malaria, dengue and zika. therefore, the emerging resistance to the widely used pyrethroid insecticides is an alarming problem for public health. herein we demonstrated the use of rna interference ...	2016	27416771
animals in the zika virus life cycle: what to expect from megadiverse latin american countries.	zika virus (zikv) was first isolated in 1947 in primates in uganda, west africa. the virus remained confined to the equatorial regions of africa and asia, cycling between infecting monkeys, arboreal mosquitoes, and occasionally humans. the zikv asiatic strain was probably introduced into brazil in or around late 2013. presently, zikv is in contact with the rich biodiversity in all brazilian biomes, bordering on other latin american countries. infections in brazilian primates have been reported r ...	2016	28005902
artificial diets for mosquitoes.	mosquito-borne diseases are responsible for more than a million human deaths every year. modern mosquito control strategies such as sterile insect technique (sit), release of insects carrying a dominant lethal (ridl), population replacement strategies (pr), and wolbachia-based strategies require the rearing of large numbers of mosquitoes in culture for continuous release over an extended period of time. anautogenous mosquitoes require essential nutrients for egg production, which they obtain thr ...	2016	28009851
diversity of cultivable midgut microbiota at different stages of the asian tiger mosquito, aedes albopictus from tezpur, india.	aedes aegypti and ae. albopictus are among the most important vectors of arboviral diseases, worldwide. recent studies indicate that diverse midgut microbiota of mosquitoes significantly affect development, digestion, metabolism, and immunity of their hosts. midgut microbiota has also been suggested to modulate the competency of mosquitoes to transmit arboviruses, malaria parasites etc. interestingly, the midgut microbial flora is dynamic and the diversity changes with the development of vectors ...	2016	27941985
flaviviruses as a cause of undifferentiated fever in sindh province, pakistan: a preliminary report.	arboviral diseases are expanding worldwide, yet global surveillance is often limited due to diplomatic and cultural barriers between nations. with human encroachment into new habitats, mosquito-borne viruses are also invading new areas. the actual prevalence of expanding arboviruses is unknown in pakistan due to inappropriate diagnosis and poor testing for arboviral diseases. the primary objective of this study was to document evidence of flavivirus infections as the cause of undifferentiated fe ...	2016	26909342
changes in the proteome of langat-infected ixodes scapularis ise6 cells: metabolic pathways associated with flavivirus infection.	ticks (family ixodidae) transmit a variety of disease causing agents to humans and animals. the tick-borne flaviviruses (tbfs; family flaviviridae) are a complex of viruses, many of which cause encephalitis and hemorrhagic fever, and represent global threats to human health and biosecurity. pathogenesis has been well studied in human and animal disease models. equivalent analyses of tick-flavivirus interactions are limited and represent an area of study that could reveal novel approaches for tbf ...	2016	26859745
the asian tiger hunts in maputo city--the first confirmed report of aedes (stegomyia) albopictus (skuse, 1895) in mozambique.	increasing evidence suggests that dengue fever is endemic in mozambique. larvae of both the afrotropical vector aedes aegypti and its subspecies, ae. aegypti formosus, have been reported from three provinces in mozambique, two of which recently experienced dengue outbreaks. despite reports of the invasive oriental vector ae. albopictus on the islands in the mozambique channel and nearby indian ocean, the species has not yet been reported in mozambique.	2016	26856329
cross-protection induced by japanese encephalitis vaccines against different genotypes of dengue viruses in mice.	dengue viruses (denvs) and japanese encephalitis virus (jev) are closely related mosquito-borne flaviviruses that cause very high global disease burdens. although cross-reactivity and cross-protection within flaviviruses have been demonstrated, the effect of jev vaccination on susceptibility to denv infection has not been well elucidated. in this study, we found that vaccination with the jev inactivated vaccine (inv) and live attenuated vaccine (lav) could induce cross-immune responses and cross ...	2016	26818736
long-lasting immune protection and other epidemiological findings after chikungunya emergence in a cambodian rural community, april 2012.	the east/central/south african genotype of chikungunya virus with the e1-a226v mutation emerged in 2011 in cambodia and spread in 2012. an outbreak of 190 cases was documented in trapeang roka, a rural village. we surveyed 425 village residents within 3-4 weeks after the outbreak, and determined the sensitivity and specificity of case definitions and factors associated with infection by chikv. self-reported clinical presentation consisted mostly of fever, rash and arthralgia. the presence of all ...	2016	26752630
behavioral response of aedes aegypti mosquito towards essential oils using olfactometer.	aedes aegypti mosquito is responsible for transmitting human diseases like dengue and chikungunya. personal or space protection with insect repellents is a practical approach to reducing human mosquito contact, thereby minimizing disease transmission. essential oils are natural volatile substances from plants used as protective measure against blood-sucking mosquitoes.	2016	27308295
screening of methanolic plant extracts against larvae of aedes aegypti and anopheles stephensi in mysore.	mosquitoes transmit serious human diseases, causing millions of death every year. vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. nine different locally available medicinally important plants suspected to posse larvicidal property were screened against fourth instar larvae of aedes aegypti and anopheles stephensi to a series of concentrations of ...	2016	27308289
insecticide resistance and management strategies in urban ecosystems.	the increased urbanization of a growing global population makes imperative the development of sustainable integrated pest management (ipm) strategies for urban pest control. this emphasizes pests that are closely associated with the health and wellbeing of humans and domesticated animals. concurrently there are regulatory requirements enforced to minimize inadvertent exposures to insecticides in the urban environment. development of insecticide resistance management (irm) strategies in urban eco ...	2016	26751480
analysis of genotype diversity and evolution of dengue virus serotype 2 using complete genomes.	dengue is one of the most common arboviral diseases prevalent worldwide and is caused by dengue viruses (genus flavivirus, family flaviviridae). there are four serotypes of dengue virus (denv-1 to denv-4), each of which is further subdivided into distinct genotypes. denv-2 is frequently associated with severe dengue infections and epidemics. denv-2 consists of six genotypes such as asian/american, asian i, asian ii, cosmopolitan, american and sylvatic. comparative genomic study was carried out t ...	2016	27635316
detection of chikungunya virus circulation using sugar-baited traps during a major outbreak in french guiana.		2016	27606960
sequence diversity of dengue virus type 2 in brain and thymus of infected interferon receptor ko mice: implications for dengue virulence.	we previously reported that a clinical isolate of dengue virus (denv) is capable of causing acute-phase systemic infection in mice harboring knockouts of the genes encoding type-i and -ii interferon ifn receptors (ifn-α/β/γr ko mice); in contrast, other virulent denv isolates exhibited slow disease progression in this mice, yielding lethal infection around 20 days post-infection (p.i.). in the present study, we sought to clarify the dynamics of slow disease progression by examining disease progr ...	2016	27903277
febrile illness diagnostics and the malaria-industrial complex: a socio-environmental perspective.	global prioritization of single-disease eradication programs over improvements to basic diagnostic capacity in the global south have left the world unprepared for epidemics of chikungunya, ebola, zika, and whatever lies on the horizon. the medical establishment is slowly realizing that in many parts of sub-saharan africa (ssa), particularly urban areas, up to a third of patients suffering from acute fever do not receive a correct diagnosis of their infection.	2016	27855644
persistence of neutralizing antibody against dengue virus 2 after 70 years from infection in nagasaki.	this study aimed to investigate the duration of humoral immune responses to dengue virus (denv) infection in japanese who experienced acute febrile illness with hemorrhagic manifestations 70 years ago, when an epidemic of dengue occurred in nagasaki, japan, from 1942 to 1944. a japanese volunteer requested serological diagnosis of denv infection in 2014 and donated blood sample to measure the antibody titer against denv by antiflavi igg indirect elisa, focus reduction neutralization test, and pl ...	2016	27493841
assessment of angelica sinensis (oliv.) diels as a repellent for personal protection against mosquitoes under laboratory and field conditions in northern thailand.	angelica sinensis (oliv.) hexane extract (ahe) has been reported as a proven and impressive repellent against laboratory-reared female aedes aegypti mosquitoes. with the aim of promoting products of plant origin as a viable alternative to conventional synthetic substances, this study was designed to transform ahe-based repellents for exploitable commercial production by enhancing their efficacy and assessing their physical and biological stability as well as repellency against mosquitoes under l ...	2016	27357395
dengue virus genome uncoating requires ubiquitination.	the process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, zika, and west nile viruses. currently, dengue virus is one of the most significant human viral pathogens transmitted by mosquitoes and is responsible for about 390 million infections every year around the world. here, we examined for the first time molecular aspect ...	2016	27353759
flavivirus ns1 protein in infected host sera enhances viral acquisition by mosquitoes.	the arbovirus life cycle involves viral transfer between a vertebrate host and an arthropod vector, and acquisition of virus from an infected mammalian host by a vector is an essential step in this process. here, we report that flavivirus nonstructural protein-1 (ns1), which is abundantly secreted into the serum of an infected host, plays a critical role in flavivirus acquisition by mosquitoes. the presence of dengue virus (denv) and japanese encephalitis virus ns1s in the blood of infected inte ...	2016	27562253
prevention and control of zika as a mosquito-borne and sexually transmitted disease: a mathematical modeling analysis.	the ongoing zika virus (zikv) epidemic in the americas poses a major global public health emergency. while zikv is transmitted from human to human by bites of aedes mosquitoes, recent evidence indicates that zikv can also be transmitted via sexual contact with cases of sexually transmitted zikv reported in argentina, canada, chile, france, italy, new zealand, peru, portugal, and the usa. yet, the role of sexual transmission on the spread and control of zikv infection is not well-understood. we i ...	2016	27312324
role of aedes aegypti (linnaeus) and aedes albopictus (skuse) in local dengue epidemics in taiwan.	aedes mosquitoes in taiwan mainly comprise aedes albopictus and ae. aegypti. however, the species contributing to autochthonous dengue spread and the extent at which it occurs remain unclear. thus, in this study, we spatially analyzed real data to determine spatial features related to local dengue incidence and mosquito density, particularly that of ae. albopictus and ae. aegypti.	2016	27829399
low sensitivity of the tourniquet test for differential diagnosis of dengue: an analysis of 28,000 trials in patients.	the aim of this study was to evaluate the utility of the tourniquet test (tt) for dengue diagnosing. to our knowledge, no previous study with such a large sample, of this duration, with as many laboratory methods referenced, or relating the results of the tt to the 2009 who classification of severity has been conducted thus far.	2016	27809813
vertical transmission of zika virus in aedes aegypti mosquitoes.	previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. in this study, designed to test whether zika virus (zikv) could be vertically transmitted, female aedes aegypti and aedes albopictus were injected with zikv, and their f1 adult progeny were tested for zikv ...	2016	27573623
unsuspected dengue as a cause of acute febrile illness in children and adults in western nicaragua.	dengue is an emerging infectious disease of global significance. suspected dengue, especially in children in nicaragua's heavily-urbanized capital of managua, has been well documented, but unsuspected dengue among children and adults with undifferentitated fever has not.	2016	27792777
vector competence of american mosquitoes for three strains of zika virus.	in 2015, zika virus (zikv; flaviviridae; flavivirus) emerged in the americas, causing millions of infections in dozens of countries. the rapid spread of the virus and the association with disease outcomes such as guillain-barré syndrome and microcephaly make understanding transmission dynamics essential. currently, there are no reports of vector competence (vc) of american mosquitoes for zikv isolates from the americas. further, it is not clear whether zikv strains from other genetic lineages ca ...	2016	27783679
a review of the mosquito species (diptera: culicidae) of bangladesh.	diseases caused by mosquito-borne pathogens remain an important source of morbidity and mortality in bangladesh. to better control the vectors that transmit the agents of disease, and hence the diseases they cause, and to appreciate the diversity of the family culicidae, it is important to have an up-to-date list of the species present in the country. original records were collected from a literature review to compile a list of the species recorded in bangladesh.	2016	27770829
zika virus (zikv).		2016	27994533
looking for inhibitors of the dengue virus ns5 rna-dependent rna-polymerase using a molecular docking approach.	the dengue virus (denv) nonstructural protein 5 (ns5) contains both an n-terminal methyltransferase domain and a c-terminal rna-dependent rna polymerase domain. polymerase activity is responsible for viral rna synthesis by a de novo initiation mechanism and represents an attractive target for antiviral therapy. the incidence of denv has grown rapidly and it is now estimated that half of the human population is at risk of becoming infected with this virus. despite this, there are no effective dru ...	2016	27784988
zika virus on a spreading spree: what we now know that was unknown in the 1950's.	zika virus (zikv) is a mosquito-borne flavivirus that is transmitted through the bite of aedes spp mosquitoes and less predominantly, through sexual intercourse. prior to 2007, zikv was associated with only sporadic human infections with minimal or no clinical manifestations. recently the virus has caused disease outbreaks from the pacific islands, the americas, and off the coast of west africa with approximately 1.62 million people suspected to be infected in more than 60 countries around the g ...	2016	27716242
full genome sequence and sfrna interferon antagonist activity of zika virus from recife, brazil.	the outbreak of zika virus (zikv) in the americas has transformed a previously obscure mosquito-transmitted arbovirus of the flaviviridae family into a major public health concern. little is currently known about the evolution and biology of zikv and the factors that contribute to the associated pathogenesis. determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative proces ...	2016	27706161
management of infection by the zika virus.	a panel of national experts was convened by the brazilian infectious diseases society in order to organize the national recommendations for the management of zika virus infection. the focus of this document is the diagnosis, both clinical and laboratorial, and appropriate treatment of the diverse manifestations of this infection, ranging from acute mild disease to guillain-barré syndrome and also microcephaly and congenital malformations.	2016	27686610
the golgi associated eri3 is a flavivirus host factor.	dengue virus (denv) is a mosquito-borne flavivirus classified into four serotypes (denv-1-4) that causes dengue fever (df), dengue hemorrhagic fever (dhf) or dengue shock syndrome (dss). an estimated 390 million people are at risk for infection with denv and there are no effective vaccines or therapeutics. we utilized rna chromatography coupled with quantitative mass spectrometry (qms) to identify host rna binding proteins (rbps) that interact with denv-2 rna. we identified eri3 (also prnpip and ...	2016	27682269
discovery of novel viruses in mosquitoes from the zambezi valley of mozambique.	mosquitoes carry a wide variety of viruses that can cause vector-borne infectious diseases and affect both human and veterinary public health. although mozambique can be considered a hot spot for emerging infectious diseases due to factors such as a rich vector population and a close vector/human/wildlife interface, the viral flora in mosquitoes have not previously been investigated. in this study, viral metagenomics was employed to analyze the viral communities in culex and mansonia mosquitoes ...	2016	27682810
genetic characterization of spondweni and zika viruses and susceptibility of geographically distinct strains of aedes aegypti, aedes albopictus and culex quinquefasciatus (diptera: culicidae) to spondweni virus.	zika virus (zikv) has extended its known geographic distribution to the new world and is now responsible for severe clinical complications in a subset of patients. while substantial genetic and vector susceptibility data exist for zikv, less is known for the closest related flavivirus, spondweni virus (sponv). both zikv and sponv have been known to circulate in africa since the mid-1900s, but neither has been genetically characterized by gene and compared in parallel. furthermore, the susceptibi ...	2016	27783682
genetically modified (gm) mosquito use to reduce mosquito-transmitted disease in the us: a community opinion survey.	mosquito-borne infectious diseases such as dengue, chikungunya, and now zika, pose a public health threat to the us, particularly florida, the gulf coast states, and hawaii. recent autochthonous transmission of dengue and chikungunya in florida, the recent dengue outbreak in hawaii, and the potential for future local spread of zika in the us, has led to the consideration of novel approaches to mosquito management. one such novel approach, the release of sterile genetically modified mosquitoes, h ...	2016	27366585
predicting dengue fever outbreaks in french guiana using climate indicators.	dengue fever epidemic dynamics are driven by complex interactions between hosts, vectors and viruses. associations between climate and dengue have been studied around the world, but the results have shown that the impact of the climate can vary widely from one study site to another. in french guiana, climate-based models are not available to assist in developing an early warning system. this study aims to evaluate the potential of using oceanic and atmospheric conditions to help predict dengue f ...	2016	27128312
spread of the invasive mosquitoes aedes aegypti and aedes albopictus in the black sea region increases risk of chikungunya, dengue, and zika outbreaks in europe.		2016	27115737
external quality assessment of dengue and chikungunya diagnostics in the asia pacific region, 2015.	to conduct an external quality assessment (eqa) of dengue and chikungunya diagnostics among national-level public health laboratories in the asia pacific region following the first round of eqa for dengue diagnostics in 2013.	2016	27508088
the mitotic spindle: linking teratogenic effects of zika virus with human genetics?	recently, an association between zika virus infection and microcephaly/ocular findings was found to be reasonable e.g. because of the demonstration that the virus was found in the brain of a fetus after presumed maternal infection. although there is no proof yet for a causal relationship, for an appropriate risk calculation efforts are urgently needed to either establish or disprove this assumption.	2016	27099632