Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antibacterial effect of butyryl alkannin from arnebia euchroma against vancomycin-resistant pathogens of enterococcus faecalis causing urinary tract infections. | this study was carried out to investigate the biomedicinal potential of a bioactive marker component, butyryl alkannin, isolated from n-hexane root extract of arnebia euchroma against various vancomycin-resistant enterococcus (vre) isolates of enterococcus faecalis causing urinary tract infections. as a result, butyryl alkannin showed significant antibacterial activity against multidrug-resistant e. faecalis pathogens of vre as minimum inhibitory concentration values which were found in the rang ... | 2015 | 25635653 |
| ef0176 and ef0177 from enterococcus faecalis v583 are substrate-binding lipoproteins involved in abc transporter mediated ribonucleoside uptake. | one of the abc transporter systems in enterococcus faecalis v583 is encoded by the ef0176-ef0180 gene cluster, which differs from orthologous operons in related bacteria in that it contains two genes putatively encoding substrate-binding proteins (sbps). these sbps, ef0176 and ef0177, have previously been identified on the surface of e. faecalis. by phenotypic studies of single and double knockout mutants, we show here that ef0176 and ef0177 are specific for ribonucleosides and, by inference, th ... | 2015 | 25635276 |
| in vitro activity of linezolid as assessed through the 2013 leader surveillance program. | the 2013 leader surveillance program monitored the in vitro activity of linezolid and comparator agents against gram-positive bacteria at 60 medical centers in the united states. a total of 7183 pathogens were contributed from 6 predetermined pathogen groups. the groups were staphylococcus aureus, coagulase-negative staphylococci, enterococci, streptococcus pneumoniae, β-hemolytic streptococci, and viridans group streptococci. the mic90 value for each of the 6 pathogen groups was 1 μg/ml. suscep ... | 2015 | 25633420 |
| ex vivo evaluation of three instrumentation techniques on e. faecalis biofilm within oval shaped root canals. | the objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with enterococcus faecalis. forty-five human lower premolars were infected with a culture of e. faecalis (atcc 29212) for 28 days. five other teeth that were neither contaminated nor instrumented were used as controls. the 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (k- ... | 2015 | 25627890 |
| update of the telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2013), when applying the revised susceptibility testing method. | a revised broth microdilution susceptibility testing method for telavancin was approved by the food and drug administration (fda). telavancin activity was assessed against gram-positive pathogens collected worldwide (2013) using the revised method. a total of 12,346 isolates from 90 sites were included as part of the telavancin international surveillance program for the americas, europe, and asia-pacific. telavancin had mic50 and mic90 values of 0.03 and 0.06 μg/ml, respectively, against staphyl ... | 2015 | 25618421 |
| dietary enterococcus faecalis lab31 improves growth performance, reduces diarrhea, and increases fecal lactobacillus number of weaned piglets. | lactic acid bacteria (lab) have been shown to enhance performance of weaned piglets. however, few studies have reported the addition of lab enterococcus faecalis as alternatives to growth promoting antibiotics for weaned piglets. this study evaluated the effects of dietary e. faecalis lab31 on the growth performance, diarrhea incidence, blood parameters, fecal bacterial and lactobacillus communities in weaned piglets. a total of 360 piglets weaned at 26 ± 2 days of age were randomly allotted to ... | 2015 | 25617897 |
| synthesis of silver nanoparticles using solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line mcf 7. | in the present study, we have synthesized silver nanoparticles by a simple and eco-friendly method using unripe fruits of solanum trilobatum. the aqueous silver ions when exposed to unripe fruits extract were reduced and stabilized over long time resulting in biosynthesis of surface functionalized silver nanoparticles. the bio-reduced silver nanoparticles were characterized by uv-visible spectroscopy, fourier transform infrared spectroscopy (ftir), scanning electron microscopy (sem), transmissio ... | 2015 | 25613692 |
| gold nanoshell-decorated silicone surfaces for the near-infrared (nir) photothermal destruction of the pathogenic bacterium e. faecalis. | catheter-related infections (cris) are associated with the formation of pathogenic biofilms on the surfaces of silicone catheters, which are ubiquitous in medicine. these biofilms provide protection against antimicrobial agents and facilitate the development of bacterial resistance to antibiotics. the application of photothermal agents on catheter surfaces is an innovative approach to overcoming biofilm-generated cris. gold nanoshells (aunss) represent a promising photothermal tool, because they ... | 2015 | 25611157 |
| antibiotic resistance prevalence in routine bloodstream isolates from children's hospitals varies substantially from adult surveillance data in europe. | surveillance of antimicrobial resistance (amr) is central for defining appropriate strategies to deal with changing amr levels. it is unclear whether childhood amr patterns differ from those detected in isolates from adult patients. | 2015 | 25607829 |
| fluoride varnish as root canal sealer: a scanning electron microscopy and bacterial penetration study. | this study was carried out to evaluate the bacterial leakage of root canal fillings when cavity varnish containing 5% fluoride (duraflur) was used as root canal sealer. | 2015 | 25598813 |
| antimicrobial activity of nanoparticle calcium hydroxide against enterococcus faecalis: an in vitro study. | enterococcus faecalis (e. faecalis) has the ability to invade the dentinal tubules and resist high ph levels. as a result, calcium hydroxide (ch) is not much effective on this bacterium. in theory, nanoparticle calcium hydroxide (nch) has smaller size and high surface area that enables it to penetrate into the deeper layers of dentin and be more effective on e. faecalis. this in vitro study was designed to compare the antimicrobial activity of nch and ch against e. faecalis. | 2015 | 25598808 |
| efficacy of 3d conforming nickel titanium rotary instruments in eliminating canal wall bacteria from oval-shaped root canals. | to evaluate the effectiveness of trushape® 3d conforming files, compared with twisted files, in reducing bacteria load from root canal walls, in the presence or absence of irrigant agitation. | 2015 | 25593001 |
| nonselective mevalonate kinase inhibitor as a novel class of antibacterial agents. | introduction. there are a few evidences about targeting isoprenoids biosynthesis pathway in bacteria for finding new antibiotics. this study was conducted to assess antibacterial effects of vanadyl sulfate (vs), one of the mevalonate kinase inhibitors to find a new target for killing bacteria. materials and methods. antibacterial effect of vs alone and in combination with glycine or edta was assessed on escherichia coli and pseudomonas aeruginosa as gram-negative and staphylococcus aureus and en ... | 2015 | 25692035 |
| the effect of diluted triple and double antibiotic pastes on dental pulp stem cells and established enterococcus faecalis biofilm. | to investigate the effect of various dilutions of antibiotic medicaments used in endodontic regeneration on the survival of human dental pulp stem cells (dpscs) and to determine their antibacterial effect against established enterococcus faecalis biofilm. | 2015 | 25689981 |
| orosomucoid 1 drives opportunistic infections through the polarization of monocytes to the m2b phenotype. | orosomucoid (orm, composed of two isoforms, orm1 and orm2) has been described as an inducer of m2 macrophages, which are cells that decrease host antibacterial innate immunities. however, it is unknown which phenotypes of m2 macrophages are induced by orm. in this study, healthy donor monocytes stimulated with orm (orm-monocytes) were characterized phenotypically and biologically. ccl1 (a biomarker of m2b macrophages) and il-10 were detected in monocyte cultures supplemented with orm1; however, ... | 2015 | 25689617 |
| growth-dependent photoinactivation kinetics of enterococcus faecalis. | to investigate how the growth stage of enterococcus faecalis affects its photoinactivation in clear water. | 2015 | 25688992 |
| [synergistic effects of lysozyme with edta-2na on antibacterial activity]. | to evaluate the synergistic antibacterial effects of lysozyme with ethylenediaminetetraacetic acid disodium salt (edta-2na) on enterococcus faecalis (e. faecalis) and porphyromonas endodontalis (p. endodontalis). | 2015 | 25686329 |
| antimicrobial peptides expressed in medicinal maggots of the blow fly lucilia sericata show combinatorial activity against bacteria. | the larvae of the common green bottle fly (lucilia sericata) produce antibacterial secretions that have a therapeutic effect on chronic and nonhealing wounds. recent developments in insect biotechnology have made it possible to use these larvae as a source of novel anti-infectives. here, we report the application of next-generation rna sequencing (rna-seq) to characterize the transcriptomes of the larval glands, crop, and gut, which contribute to the synthesis of antimicrobial peptides (amps) an ... | 2015 | 25666157 |
| bioluminescence based biosensors for quantitative detection of enterococcal peptide-pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection. | enterococcus faecalis is a significant threat in the nosocomial setting due to the emergence of isolates that are multi-antibiotic resistant, refractory to the available therapies and equipped with a variety of pathogenicity determinants. this bacterium uses quorum-sensing systems to regulate its physiological processes, including the expression of virulence traits, to adapt and proliferate within a host. here, we describe the construction and application of two bioluminescence-based reporter sy ... | 2015 | 25661457 |
| behavior of listeria monocytogenes in a multi-species biofilm with enterococcus faecalis and enterococcus faecium and control through sanitation procedures. | the formation of mono-species biofilm (listeria monocytogenes) and multi-species biofilms (enterococcus faecium, enterococcus faecalis, and l. monocytogenes) was evaluated. in addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. the biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39°c) and contact times (0, 1, 2, 4, 6 and 8 days). in all tests, at 7°c, the microbial counts were below 0.4 ... | 2015 | 25655573 |
| enterococcus faecalis attenuates the differentiation of macrophages into osteoclasts. | enterococcus faecalis is closely associated with refractory apical periodontitis, manifesting periapical lesions and alveolar bone loss. macrophages playing an important role in the induction of inflammation can differentiate into bone-resorbing osteoclasts. in the present study, we investigated the effect of e. faecalis on the differentiation and function of macrophages as osteoclast precursors. | 2015 | 25649294 |
| extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins. | sulfated polysaccharides were extracted from gray triggerfish (gtsp) and smooth hound (shsp) skins. their chemical and physical characteristics were determined using x-ray diffraction and infrared spectroscopic analysis. the antibacterial activities of gtsp and shsp against listeria monocytogenes (atcc 43251), staphylococcus aureus (atcc 25923), enterococcus faecalis (atcc 29212), escherichia coli (atcc 25922), salmonella enterica (atcc 43972) and enterobacter sp were evaluated by determining cl ... | 2015 | 25647621 |
| antibiofilm efficacy evaluation of a bioelectric dressing in mono- and multi-species biofilms. | chronic infections are often related to the formation of single or polymicrobial biofilms, which lead to hard-to-treat infections, difficult wound management, and recurrent infections. in this study, we evaluated in vitro antibiofilm properties of a silver and zinc bioelectric dressing (procellera). | 2015 | 25647504 |
| horizontal transfer of antibiotic resistance from enterococcus faecium of fermented meat origin to clinical isolates of e. faecium and enterococcus faecalis. | enterococcus species are part of the normal intestinal flora of a large number of mammals including humans and consequently, they can be used as indicators of faecal contamination in food and water for human consumption. their presence in large numbers in foods may indicate a lapse in sanitation and their ability to serve as a genetic reservoir of transferable antibiotic resistance is of concern. in the present study, enterococcus spp., isolated from commercially fermented meat and human clinica ... | 2015 | 25647243 |
| detection of vancomycin resistances in enterococci within 3 ½ hours. | vancomycin resistant enterococci (vre) constitute a challenging problem in health care institutions worldwide. novel methods to rapidly identify resistances are highly required to ensure an early start of tailored therapy and to prevent further spread of the bacteria. here, a spectroscopy-based rapid test is presented that reveals resistances of enterococci towards vancomycin within 3.5 hours. without any specific knowledge on the strain, vre can be recognized with high accuracy in two different ... | 2015 | 25645753 |
| β-lactam combinations with daptomycin provide synergy against vancomycin-resistant enterococcus faecalis and enterococcus faecium. | enterococcus faecalis (efc) and enterococcus faecium (efm) are frequently resistant to vancomycin and β-lactams (bls). in vitro data suggest synergy between several bls and glycopeptides or lipopeptides against resistant pathogens. our objective was to conduct combination mic and time-kill experiments to evaluate bl synergy with daptomycin against enterococci. | 2015 | 25645208 |
| novel coating of surgical suture confers antimicrobial activity against porphyromonas gingivalis and enterococcus faecalis. | the oral cavity is colonized by >10(9) bacteria, many of which can increase heart disease risk when seeded into the bloodstream. most dentoalveolar surgeries require the use of surgical sutures. suture placement and removal can increase the risk of postoperative infection and bacteremia. the aim of this study is to evaluate the antimicrobial activity of a novel quaternary ammonium compound, k21, when coated on different suture materials. | 2015 | 25703732 |
| ultrasonic activation and chemical modification of photosensitizers enhances the effects of photodynamic therapy against enterococcus faecalis root-canal isolates. | the aim of this study was to evaluate the effect of photodynamic therapy (pdt) on enterococcus faecalis biofilms in artificially infected root canals using modified photosensitizers and passive ultrasonic activation. | 2015 | 25703104 |
| biofilms of enterococcus faecalis and enterococcus faecium isolated from the processing of ricotta and the control of these pathogens through cleaning and sanitization procedures. | the biofilm formation of enterococcus faecalis and enterococcus faecium isolated from the processing of ricotta on stainless steel coupons was evaluated, and the effect of cleaning and sanitization procedures in the control of these biofilms was determined. the formation of biofilms was observed while varying the incubation temperature (7, 25 and 39°c) and time (0, 1, 2, 4, 6 and 8 days). at 7°c, the counts of e. faecalis and e. faecium were below 2 log10 cfu/cm(2). for the temperatures of 25 an ... | 2015 | 25702883 |
| effects of ciprofloxacin-containing scaffolds on enterococcus faecalis biofilms. | antibiotic-containing polymer-based nanofibers (hereafter referred to as scaffolds) have demonstrated great potential for their use in regenerative endodontics from both an antimicrobial and cytocompatibility perspective. this study sought to evaluate in vitro the effects of ciprofloxacin (cip)-containing polymer scaffolds against enterococcus faecalis biofilms. | 2015 | 25698261 |
| phage-mediated dispersal of biofilm and distribution of bacterial virulence genes is induced by quorum sensing. | the microbiome and the phage meta-genome within the human gut are influenced by antibiotic treatments. identifying a novel mechanism, here we demonstrate that bacteria use the universal communication molecule ai-2 to induce virulence genes and transfer them via phage release. high concentrations (i.e. 100 μm) of ai-2 promote dispersal of bacteria from already established biofilms, and is associated with release of phages capable of infecting other bacteria. enterococcus faecalis v583δabc harbour ... | 2015 | 25706310 |
| is it safe to reuse dental implant healing abutments sterilized and serviced by dealers of dental implant manufacturers? an in vitro sterility analysis. | this study was undertaken to investigate the sterility of used healing abutments sterilized and serviced by dealers of dental implant manufacturers. | 2015 | 25706262 |
| in vitro activity of gentamicin, vancomycin or amikacin combined with edta or l-arginine as lock therapy against a wide spectrum of biofilm-forming clinical strains isolated from catheter-related infections. | treatment of catheter-related bloodstream infections (crbsi) is hampered by the characteristic tolerance of bacterial biofilms towards antibiotics. our objective was to study the effect of the combination of antibiotics and the alkaline amino acid l-arginine or the cation chelator edta on the bacterial killing of in vitro biofilms formed by an array of clinical strains responsible for crbsi and representative of epidemiologically relevant bacterial species. | 2015 | 25712314 |
| spread of an enterococcus faecalis sequence type 6 (cc2) clone in patients undergoing selective decontamination of the digestive tract. | enterococcus faecalis (e. faecalis) is a common cause of nosocomial infection in immunocompromised patients. the presence and dissemination of high-risk clonal complexes, such as cc2, is an ongoing problem in hospitals. the aim of this work was to characterize 24 e. faecalis isolates from icu patients undergoing selective decontamination of the digestive tract (sdd) by phenotypical (antimicrobial susceptibility) and genotypical (presence of virulence genes, rapd-pcr and mlst) methods. our result ... | 2015 | 25712203 |
| prevalence, resistance patterns, and risk factors for antimicrobial resistance in bacteria from retail chicken meat in colombia. | as a step toward implementing the colombian integrated program for antimicrobial resistance surveillance (coipars), this study aimed to establish the baseline antimicrobial resistance patterns of salmonella serovars, escherichia coli, and enterococcus spp. isolates in retail poultry meat from independent stores and from a main chain distributor center. mics of the isolates were determined for antimicrobials used both in humans and animals, using an automated system. salmonella serovars were isol ... | 2015 | 25836401 |
| enterococcus faecalis gelatinase mediates intestinal permeability via protease-activated receptor 2. | microbial protease-mediated disruption of the intestinal epithelium is a potential mechanism whereby a dysbiotic enteric microbiota can lead to disease. this mechanism was investigated using the colitogenic, protease-secreting enteric microbe enterococcus faecalis. caco-2 and t-84 epithelial cell monolayers and the mouse colonic epithelium were exposed to concentrated conditioned media (ccm) from e. faecalis v583 and e. faecalis lacking the gelatinase gene (gele). the flux of fluorescein isothio ... | 2015 | 25916983 |
| anaerobic degradation of tannins in acacia nilotica pods by enterococcus faecalis in co-culture with ruminal microbiota. | 2015 | 25833679 | |
| cytotoxic and antibacterial angucycline- and prodigiosin-analogues from the deep-sea derived streptomyces sp. scsio 11594. | two new c-glycoside angucyclines, marangucycline a (1) and marangucycline b (2), along with three known compounds, dehydroxyaquayamycin (3), undecylprodigiosin (4) and metacycloprodigiosin (5), have been identified as products of the deep-sea sediment strain streptomyces sp. scsio 11594. new structures were elucidated on the basis of hresims, 1d and 2d nmr analyses and comparisons to previously reported datasets. compounds 2 and 4 displayed in vitro cytotoxicity against four cancer cell lines a5 ... | 2015 | 25786061 |
| antimicrobial activity of plectasin nz2114 in combination with cell wall targeting antibiotics against vana-type enterococcus faecalis. | antimicrobial peptide plectasin targeting bacterial cell wall precursor lipid ii has been reported to be active against benzylpenicillin-resistant streptococcus pneumoniae but less potent against vancomycin-resistant enterococci than their susceptible counterparts. the aim of this work was to test plectasin nz2114 in combination with cell wall targeting antibiotics on vancomycin-resistant enterococcus faecalis. the activity of antibiotic combinations was evaluated against vana-type vancomycin-re ... | 2015 | 25785733 |
| hydrogel-based microfluidic incubator for microorganism cultivation and analyses. | this work presents an array of microfluidic chambers for on-chip culturing of microorganisms in static and continuous shear-free operation modes. the unique design comprises an in-situ polymerized hydrogel that forms gas and reagent permeable culture wells in a glass chip. utilizing a hydrophilic substrate increases usability by autonomous capillary priming. the thin gel barrier enables efficient oxygen supply and facilitates on-chip analysis by chemical access through the gel without introducin ... | 2015 | 25784966 |
| increased membrane surface positive charge and altered membrane fluidity leads to cationic antimicrobial peptide resistance in enterococcus faecalis. | to understand the role of cell membrane phospholipids during resistance development to cationic antimicrobial peptides (camps) in enterococcus faecalis, gradual dose-dependent single exposure pediocin-resistant (pedr) mutants of e. faecalis (efv2.1, efv3.1, efv3.2, efv4.1, efv4.2, efv5.1, efv5.2 and efv5.3), conferring simultaneous resistance to other camps, selected in previous study were characterized for cell membrane phospholipid head-groups and fatty acid composition. the involvement of pho ... | 2015 | 25782727 |
| unraveling antimicrobial resistance genes and phenotype patterns among enterococcus faecalis isolated from retail chicken products in japan. | multidrug-resistant enterococci are considered crucial drivers for the dissemination of antimicrobial resistance determinants within and beyond a genus. these organisms may pass numerous resistance determinants to other harmful pathogens, whose multiple resistances would cause adverse consequences. therefore, an understanding of the coexistence epidemiology of resistance genes is critical, but such information remains limited. in this study, our first objective was to determine the prevalence of ... | 2015 | 25781022 |
| a genomic virulence reference map of enterococcus faecalis reveals an important contribution of phage03-like elements in nosocomial genetic lineages to pathogenicity in a caenorhabditis elegans infection model. | in the present study, the commensal and pathogenic host-microbe interaction of enterococcus faecalis was explored using a caenorhabditis elegans model system. the virulence of 28 e. faecalis isolates representing 24 multilocus sequence types (mlsts), including human commensal and clinical isolates as well as isolates from animals and of insect origin, was investigated using c. elegans strain glp-4 (bn2ts); sek-1 (km4). this revealed that 6 e. faecalis isolates behaved in a commensal manner with ... | 2015 | 25776747 |
| influence of working length and apical preparation size on apical bacterial extrusion during reciprocating instrumentation. | to evaluate the influence of reciprocating single-file instrumentation with different working lengths (wl) and apical preparation sizes on apical bacterial extrusion. | 2015 | 25087922 |
| mckittrick-wheelock syndrome complicated by enterococcus faecalis endocarditis: a unique combination. | 2015 | 25112151 | |
| mutational analysis of critical residues of fad-independent catabolic acetolactate synthase from enterococcus faecalis v583. | catabolic acetolactate synthase (cals) from enterococcus faecalis is a fad-independent enzyme, which catalyzes the condensation of two molecules of pyruvate to produce acetolactate. mutational and kinetic analyses of variants suggested the importance of h111, q112, and q411 residues for catalysis in cals. the wild-type and variants were expressed as equally soluble proteins and co-migrated to a size of 60 kda on sds-page. importantly, h111 in cals, which is widely present as phenylalanine in man ... | 2015 | 25128823 |
| relationships between the antibacterial activity of sodium hypochlorite and treatment time and biofilm age in early enterococcus faecalis biofilms. | to determine the relationships between the antibacterial activity of naocl and treatment time and biofilm age in early enterococcus faecalis biofilms using a linear fitting procedure. | 2015 | 25156394 |
| recurrent linezolid-resistant enterococcus faecalis infection in a patient with pneumonia. | it has been reported that lzd-resistant enterococcus in the gastrointestinal tract of mice colonizes persistently and shows variable minimum inhibitor concentration (mic) values. however, the colonization characteristics of enterococcus with lzd resistance in patients remain elusive. here, we report the case of a patient with recurrent pneumonia due to infection with lzd-resistant enterococcus faecalis strains. the colonization characteristics of the strains isolated from this patient were analy ... | 2015 | 25236388 |
| construction of a chimeric lysin ply187n-v12c with extended lytic activity against staphylococci and streptococci. | developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. in the present work, a novel chimeric lysin (ply187n-v12c) was constructed by fusing the catalytic domain (ply187n) of the bacteriophage lysin ply187 with the cell binding domain (146-314aa, v12c) of the lysin plyv12. the results showed that the chimeric lysin ply187n-v12c had not only lytic activity similar to ply187n against staphylococcal strains but al ... | 2015 | 25219798 |
| oligomerization of esculin improves its antibacterial activity and modulates antibiotic resistance. | in this particular study, the antibacterial activity of esculin and oligomer fractions was assessed. mic values of esculin and its oligomer fractions as well as of some antibiotics against gram-positive and gram-negative strains and against escherichia coli multiresistant variants were determined by the standard broth microdilution method. both esculin and oligoesculin fractions exhibited antibacterial effect against reference strains; staphylococcus aureus, enterococcus faecalis, salmonella ent ... | 2015 | 25248728 |
| transcriptomic response of enterococcus faecalis v583 to low hydrogen peroxide levels. | enterococcus faecalis is a gram-positive commensal bacterium inhabiting the gastrointestinal tracts of human and other mammals, but is also increasingly recognized as an opportunistic human pathogen. oxidative stress is one of the major challenges encountered by enterococci, both in their natural environment and during infection. in this paper, we evaluated the transcriptomic response of e. faecalis to oxidative stress, and showed that transcript abundance was reduced for 93 genes and increased ... | 2015 | 25245959 |
| ceftobiprole and ampicillin increase daptomycin susceptibility of daptomycin-susceptible and -resistant vre. | the synergistic combination of daptomycin plus ampicillin has proven to be effective against vre including daptomycin-non-susceptible strains. ceftobiprole is a cephalosporin with broad binding affinity for enterococcal pbp subtypes including pbp5. given the synergy between β-lactams and daptomycin against vre, it was of interest to determine whether ceftobiprole offered any synergistic advantage with daptomycin compared with ampicillin. | 2015 | 25304643 |
| the type i toxin-antitoxin par locus from enterococcus faecalis plasmid pad1: rna regulation by both cis- and trans-acting elements. | the pad1 par determinant was the first type i toxin-antitoxin system identified in gram-positive bacteria and has recently been shown to be the prototype of a family of loci that is widespread in these organisms. all family members have (i) convergently transcribed toxin message and regulatory rnas, (ii) three non-contiguous complementary regions for potential interaction, and (iii) intramolecular structures within the toxin message that modulate translation and transcript stability. therefore, ... | 2015 | 25312777 |
| distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using rna- and dna-based quantitative pcr assays. | the identification of fecal pollution sources is commonly carried out using dna-based methods. however, there is evidence that dna can be associated with dead cells or present as "naked dna" in the environment. furthermore, it has been shown that rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) assays can be more sensitive than rrna gene-based qpcr assays since metabolically active cells usually contain higher numbers of ribosomes than quiescent cells. to this end, we compared the ... | 2015 | 25326295 |
| photodynamic killing of enterococcus faecalis in dentinal tubules using mthpc incorporated in liposomes and invasomes. | the present in vitro study investigates the antimicrobial photodynamic efficiency of the photosensitizer 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (mthpc) incorporated in liposomes (lip) and highly flexible invasomes (inv) on the endodontopathogenic species enterococcus faecalis in infected dental root canals. | 2015 | 24950607 |
| the surface rhamnopolysaccharide epa of enterococcus faecalis is a key determinant of intestinal colonization. | enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients. the pathogenesis of e. faecalis infection relies in part on its capacity to colonize the gut. following disruption of intestinal homeostasis, e. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream. to identify and characterize e. faecalis genes that are key to intestinal colonization, our strategy consisted in scr ... | 2015 | 25035517 |
| potential for nosocomial transmission of enterococcus faecalis from surfaces in dental operatories. | to assess the potential for nosocomial transmission of enterococcus faecalis during root canal treatment by measuring its occurrence on surfaces in dental operatories in relation to the efficacy of disinfection routines. | 2015 | 25066305 |
| formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (apdt): studies on curcumin and curcuminoids lii. | bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (apdt) are needed. curcumin is under investigation as a potential photosensitizer in apdt. | 2015 | 24842546 |
| assessment of dentinal tubule invasion capacity of enterococcus faecalis under stress conditions ex vivo. | to investigate the dentinal tubule invasion capacity of enterococcus faecalis under alkaline and energy starvation stress conditions. | 2015 | 24872016 |
| characterisation of the antibacterial effect of polyethyleneimine nanoparticles in relation to particle distribution in resin composite. | to characterise the antibacterial effect of resin composite incorporating cross-linked quaternised polyethyleneimine (qpei) nanoparticles in relation to their distribution in the bulk material. | 2015 | 24881908 |
| production of lactic acid using a new homofermentative enterococcus faecalis isolate. | lactic acid is an intermediate-volume specialty chemical for a wide range of food and industrial applications such as pharmaceuticals, cosmetics and chemical syntheses. although lactic acid production has been well documented, improved production parameters that lead to reduced production costs are always of interest in industrial developments. in this study, we describe the production of lactic acid at high concentration, yield and volumetric productivity utilizing a novel homofermentative, fac ... | 2015 | 24894833 |
| modified lactic acid bacteria detect and inhibit multiresistant enterococci. | we designed lactococcus lactis to detect enterococcus faecalis. upon detection, l. lactis produce and secrete antienterococcal peptides. the peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of l. lactis. the enterococcal sex pheromone ccf10 serves as the signal for detection. expression vectors derived from pcf10, a ccf10-responsive e. faecalis sex-pheromone conjugative plasmid, were engineered in l. lactis for the detection system. recombinant host strain ... | 2015 | 24896372 |
| antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or er:yag laser in vitro. | to investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of enterococcus faecalis in vitro. | 2015 | 25080240 |
| autoimmune neutropenia of infancy with recurrent urinary tract infections: a case report. | autoimmune neutropenia of infancy is characterized by minor intercurrent infections despite severe neutropenia; severe bacterial infections are uncommon. an infant developed recurrent urinary tract infections at 9 and 11 months of age. the identified uropathogens were escherichia coli and enterococcus faecalis, respectively. empirical treatment with carbapenems, as broad-spectrum antibiotics, promptly resolved the infection without sequelae. febrile neutropenic children with cancer and autoimmun ... | 2015 | 24942028 |
| commensal bacteria drive endogenous transformation and tumour stem cell marker expression through a bystander effect. | commensal bacteria and innate immunity play a major role in the development of colorectal cancer (crc). we propose that selected commensals polarise colon macrophages to produce endogenous mutagens that initiate chromosomal instability (cin), lead to expression of progenitor and tumour stem cell markers, and drive crc through a bystander effect. | 2015 | 24906974 |
| collagen degradation and mmp9 activation by enterococcus faecalis contribute to intestinal anastomotic leak. | even under the most expert care, a properly constructed intestinal anastomosis can fail to heal, resulting in leakage of its contents, peritonitis, and sepsis. the cause of anastomotic leak remains unknown, and its incidence has not changed in decades. we demonstrate that the commensal bacterium enterococcus faecalis contributes to the pathogenesis of anastomotic leak through its capacity to degrade collagen and to activate tissue matrix metalloproteinase 9 (mmp9) in host intestinal tissues. we ... | 2015 | 25947163 |
| in vitro effectiveness of brazilian brown propolis against enterococcus faecalis. | the aim of this study was to evaluate the in vitro antimicrobial activity of brazilian brown propolis as an intracanal medication against enterococcus faecalis. thirty dentin discs prepared from intact freshly extracted bovine maxillary central incisors were infected with e. faecalis for 21 days. the specimens were distributed into six groups according to the medicament used as follows: g1- calcium hydroxide paste; g2- carbowax 400 (control group); g3- 20% brown propolis paste; g4- 40% brown pro ... | 2015 | 25992787 |
| exploration of the mechanisms of biofilm formation by enterococcus faecalis in glucose starvation environments. | enterococcus faecalis is the most common single species present in teeth after failed root canal therapy. this is mainly due to its ability to maintain viability for a long time in filled root canals where nutrients are normally sparse. the aim of this study was to explore the mechanism of e. faecalis survival and biofilm formation in glucose-starved environments. | 2015 | 25990488 |
| genome modification in enterococcus faecalis og1rf assessed by bisulfite sequencing and single-molecule real-time sequencing. | enterococcus faecalis is a gram-positive bacterium that natively colonizes the human gastrointestinal tract and opportunistically causes life-threatening infections. multidrug-resistant (mdr) e. faecalis strains have emerged, reducing treatment options for these infections. mdr e. faecalis strains have large genomes containing mobile genetic elements (mges) that harbor genes for antibiotic resistance and virulence determinants. bacteria commonly possess genome defense mechanisms to block mge acq ... | 2015 | 25825433 |
| bacterial contamination in tips of electrocautery devices during total hip arthroplasty. | surgical equipment can become contaminated during surgery. it is unknown if electrocautery tips can become contaminated in clean orthopedic procedures despite the produced heat. therefore, we conducted a prospective study to address this concern. the tips from 25 primary and 25 aseptic revision thas were collected and an additional 5 sterile tips served as negative controls. aerobic and anaerobic cultures were incubated for a minimum of 3 days. there were 3 positive cultures (6%); one in primary ... | 2015 | 25817186 |
| matrix metalloproteinase inhibitor as an antimicrobial agent to eradicate enterococcus faecalis biofilm. | successful endodontic treatment outcomes require new strategies for the complete eradication of microbial biofilms in the root canal system. matrix metalloproteinases (mmps) are essential enzymes in microbial cell growth and homeostasis, and they require transition metal ion cofactors to function. targeting mmp activity also preserves dentin collagen integrity. in this study, 1,10-phenanthroline-5,6-dione (phendione), a metal chelator, was tested as a potentially novel antimicrobial agent agains ... | 2015 | 25814242 |
| optimizing antibiotic therapy of bacteremia and endocarditis due to staphylococci and enterococci: new insights and evidence from the literature. | gram-positive cocci are a well-recognised major cause of nosocomial infection worldwide. bloodstream infections due to methicillin-resistant staphylococcus aureus, methicillin-resistant coagulase-negative staphylococci, and multi-drug resistant enterococci are a cause of concern for physicians due to their related morbidity and mortality rates. aim of this article is to review the current state of knowledge regarding the management of bsi caused by staphylococci and enterococci, including infect ... | 2015 | 25813608 |
| bactericidal activity of curcumin i is associated with damaging of bacterial membrane. | curcumin, an important constituent of turmeric, is known for various biological activities, primarily due to its antioxidant mechanism. the present study focused on the antibacterial activity of curcumin i, a significant component of commercial curcumin, against four genera of bacteria, including those that are gram-positive (staphylococcus aureus and enterococcus faecalis) and gram-negative (escherichia coli and pseudomonas aeruginosa). these represent prominent human pathogens, particularly in ... | 2015 | 25811596 |
| antimicrobial activity of apitoxin, melittin and phospholipase a₂ of honey bee (apis mellifera) venom against oral pathogens. | in this work, we used the minimum inhibitory concentration (mic) technique to evaluate the antibacterial potential of the apitoxin produced by apis mellifera bees against the causative agents of tooth decay. apitoxin was assayed in natura and in the commercially available form. the antibacterial actions of the main components of this apitoxin, phospholipase a2, and melittin were also assessed, alone and in combination. the following bacteria were tested: streptococcus salivarius, s. sobrinus, s. ... | 2015 | 25806982 |
| biodiversity and γ-aminobutyric acid production by lactic acid bacteria isolated from traditional alpine raw cow's milk cheeses. | "nostrano-cheeses" are traditional alpine cheeses made from raw cow's milk in trentino-alto adige, italy. this study identified lactic acid bacteria (lab) developing during maturation of "nostrano-cheeses" and evaluated their potential to produce γ-aminobutyric acid (gaba), an immunologically active compound and neurotransmitter. cheese samples were collected on six cheese-making days, in three dairy factories located in different areas of trentino and at different stages of cheese ripening (24 ... | 2015 | 25802859 |
| evaluation of apically extruded bacteria associated with different nickel-titanium systems. | the aim of this in vitro study was to evaluate the amount of apically extruded bacteria associated with several root canal preparation systems. | 2015 | 25799537 |
| enterococcus species diversity and molecular characterization of biomarker genes in enterococcus faecalis in port blair bay, andaman and nicobar islands, india. | this study was performed to evaluate the abundance and diversity of enterococcus sp. and the distribution of biomarker genes in enterococcus faecalis in port blair bay, andaman and nicobar islands. the enterococcus sp. densities at the seven sampling stations were highly influenced by tidal fluctuations and season. the distributions and diversities of species varied in the inner and outer regions of port blair bay. among the 1816 total isolates, the occurrence of fecal enterococcus was high (1.7 ... | 2015 | 25798888 |
| antibacterial and antioxidant xanthones and benzophenone from garcinia smeathmannii. | a new prenylated xanthone, 1,3,5,8-tetrahydroxy-2-(3-methybut-2-enyl)-4-(3,7-dimethylocta-2,6-dienyl) xanthone (1), and a new benzophenone (2), together with four known xanthone derivatives, cheffouxanthone (3), smeathxanthone a (4), smeathxanthone b (5), ananixanthone (6), and two pentacyclic triterpenes, epi-friedelinol (7) and friedelin (8), were isolated from the stem bark of garcinia smeathmannii. the structures of the compounds were elucidated on the basis of 1d and 2d nmr experiments, and ... | 2015 | 25798641 |
| detection of vancomycin-resistant enterococcus faecalis st6-vanb2 and e. faecium st915-vana in faecal samples of wild rattus rattus in spain. | the detection of vancomycin-resistant-enterococci (vre) among wild animals represents a worrisome public health concern. the objectives of the study were to determine the possible presence of vre in faecal samples of wild small mammals in spain, to characterize the vancomycin resistance mechanisms and genetic lineages of recovered isolates and to know the diversity of enterococcal species in these animals. a total of 155 faecal samples from small mammals were inoculated in slanetz-bartley agar s ... | 2015 | 25795517 |
| antimicrobial activity of seven essential oils from iranian aromatic plants against common causes of oral infections. | over the past two decades, there has been a growing trend in using oral hygienic products originating from natural resources such as essential oils (eos) and plant extracts. seven aromatic plants used in this study are among popular traditional iranian medicinal plants with potential application in modern medicine as anti-oral infectious diseases. | 2015 | 25793100 |
| rapid fluorescence-based measurement of toxicity in anaerobic digestion. | a rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. by initially using a pure facultative anaerobic strain, enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (pcp). the technique revealed significant metabolic changes in enterococcus faecalis with a pcp spike ranging from 0.05 to 100 mg/l, and ... | 2015 | 25768985 |
| could β-hemolytic, group b enterococcus faecalis be mistaken for streptococcus agalactiae? | a β-hemolytic enterococcus faecalis strain agglutinating lancefield group a, b, c, d, f, and g antisera was observed from a rectovaginal swab, in the context of antenatal screening for streptococcus agalactiae (group b streptococcus [gbs]). this is the first multi-lancefield antisera-agglutinating isolate of this species, and it raised particular concern, as it may mimic gbs, leading to false reporting and useless receipt of intrapartum antibiotics. | 2015 | 25766004 |
| re: enterococcus faecalis subverts and invades the host urothelium in patients with chronic urinary tract infection. | 2015 | 25765392 | |
| bacteriological findings and antimicrobial susceptibility pattern of isolated pathogens from visual threatening ocular infections. | to study the profiles and antimicrobial susceptibility pattern of isolated bacteria from visual threatening ocular infection. | 2015 | 25764616 |
| fluoroquinolone resistance in bacteremic and low risk febrile neutropenic patients with cancer. | the low risk febrile neutropenic patients with multinational association for supportive care in cancer (mascc) score of more than 20 are recommended to be treated with fluoroquinolone-based oral treatment by the national comprehensive cancer network (nccn) guideline. this recommendation relies, at least partially, on the high sensitivity of the blood culture isolates to fluoroquinolone in clinical trials conducted in western countries. whether this also applies in middle or low income countries ... | 2015 | 25763661 |
| antimicrobial action of oleanolic acid on listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. | this study investigated the antimicrobial action of oleanolic acid against listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. to determine the cytotoxicity of oleanolic acid, hep-2 cells were incubated with oleanolic acid at 37°c. mics (minimal inhibition concentrations) for l. monocytogenes, e. faecium, and e. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid a ... | 2015 | 25756202 |
| β-lactams enhance daptomycin activity against vancomycin-resistant enterococcus faecalis and enterococcus faecium in in vitro pharmacokinetic/pharmacodynamic models. | enterococcus faecalis and enterococcus faecium are frequently resistant to vancomycin and β-lactams. in enterococcal infections with reduced glycopeptide susceptibility, combination therapy is often administered. our objective was to conduct pharmacokinetic/pharmacodynamic (pk/pd) models to evaluate β-lactam synergy with daptomycin (dap) against resistant enterococci. one e. faecalis strain (r6981) and two e. faecium strains (r6370 and 8019) were evaluated. dap mics were obtained. all strains we ... | 2015 | 25753639 |
| fluid dynamics and biofilm removal generated by syringe-delivered and 2 ultrasonic-assisted irrigation methods: a novel experimental approach. | thorough understanding of fluid dynamics in root canal irrigation and corresponding antibiofilm capacity will support improved disinfection strategies. this study aimed to develop a standardized, simulated root canal model that allows real-time analysis of fluid/irrigation dynamics and its correlation with biofilm elimination. | 2015 | 25749254 |
| ag-loaded mesoporous bioactive glasses against enterococcus faecalis biofilm in root canal of human teeth. | ag-loaded mesoporous bioactive glass (ag-mbg) powders were synthesized and characterized. the ions release of ag-mbgs in tris-hcl and the ph stability of simulated body fluids after immersing ag-mbgs were tested. root canals were inoculated with enterococcus faecalis for 4 weeks, and the antibacterial activity of mbgs, ag-mbgs and calcium hydroxide against e. faecalis biofilm were evaluated. results showed that ag-mbgs possessed highly ordered mesoporous structure with silver nanoparticles depos ... | 2015 | 25748459 |
| [analysis of pathogenic bacteria and drug resistance in neonatal purulent meningitis]. | to study the clinical characteristics, pathogenic bacteria, and antibiotics resistance of neonatal purulent meningitis in order to provide the guide for early diagnosis and appropriate treatment. | 2015 | 25748405 |
| molecular mechanisms of resistance to human pathogenic bacteria in caenorhabditis elegans by mev-1 mediated oxidative stress. | both mutation and knockdown in mev-1 gene render caenorhabditis elegans susceptibility to enterococcus faecalis infection. however, the mechanisms by which of mev-1 defects pathogen resistance remain unclear. here we show that mev-1rnai causes a dramatic decrease in oxidative stress and antioxidant enzyme expressions, thereby leading to increased e. faecalis accumulation in nematode intestine. mitochondrial superoxide change after infection induced these oxidative responses through daf-16 activi ... | 2015 | 25747713 |
| new antimicrobial contact catalyst killing antibiotic resistant clinical and waterborne pathogens. | microbial growth on medical and technical devices is a big health issue, particularly when microorganisms aggregate to form biofilms. moreover, the occurrence of antibiotic-resistant bacteria in the clinical environment is dramatically growing, making treatment of bacterial infections very challenging. in search of an alternative, we studied a novel antimicrobial surface coating based on micro galvanic elements formed by silver and ruthenium with surface catalytic properties. the antimicrobial c ... | 2015 | 25746238 |
| the effect of indomethacin and betamethasone on the cytokine response of human neonatal mononuclear cells to gram-positive bacteria. | intrauterine infections with gram-positive bacteria pose a serious threat to neonates since they can result in neonatal sepsis, induce a fetal inflammatory response and also cause preterm birth. despite intensive care, prematurity remains a leading cause of neonatal death, and is often accompanied by a number of morbidities. in order to prevent premature birth, tocolytic agents like indomethacin are administered. betamethasone is used to promote lung maturation and prevent respiratory distress s ... | 2015 | 25743243 |
| whole-genome mapping of 5' rna ends in bacteria by tagged sequencing: a comprehensive view in enterococcus faecalis. | enterococcus faecalis is the third cause of nosocomial infections. to obtain the first snapshot of transcriptional organizations in this bacterium, we used a modified rna-seq approach enabling to discriminate primary from processed 5' rna ends. we also validated our approach by confirming known features in escherichia coli. we mapped 559 transcription start sites (tsss) and 352 processing sites (psss) in e. faecalis. a blind motif search retrieved canonical features of siga- and sign-dependent p ... | 2015 | 25737579 |
| the fsr quorum-sensing system and cognate gelatinase orchestrate the expression and processing of proprotein ef_1097 into the mature antimicrobial peptide enterocin o16. | a novel antimicrobial peptide designated enterocin o16 was purified from enterococcus faecalis. mass spectrometry showed a monoisotopic mass of 7,231 da, and n-terminal edman degradation identified a 29-amino-acid sequence corresponding to residues 90 to 119 of the ef_1097 protein. bioinformatic analysis showed that enterocin o16 is composed of the 68 most c-terminal residues of the ef_1097 protein. introduction of an in-frame isogenic deletion in the ef1097 gene abolished the production of ente ... | 2015 | 25733609 |
| ec300: a phage-based, bacteriolysin-like protein with enhanced antibacterial activity against enterococcus faecalis. | bacteriophage lytic enzymes, either endolysins or virion-associated lysins, have been receiving considerable attention as potential antibacterial agents, particularly for the combat of antibiotic-resistant gram-positive pathogens. a conclusion that easily emerges from the careful analysis of a great number of reports on the field is that the activity of phage lytic enzymes is rarely studied in conditions that support robust growth of the target bacteria. here, we report the construction and stud ... | 2015 | 25728445 |
| the sfani restriction-modification system from enterococcus faecalis neb215 is located on a putative mobile genetic element. | a type iis restriction-modification (r-m) system sfani from enterococcus faecalis neb215 has been characterized. the sfanim gene was cloned by the methylase selection method. methyltransferase sfani, a protein of 695 amino acids, consists of two domains responsible for different dna-strand recognition and modification, and a putative dna-binding hth domain located in the n-terminal part of the protein. the sfanir gene, located adjacent to the gene of the cognate modification methyltransferases, ... | 2015 | 25724535 |
| validation of a novel rinse and filtration method for efficient processing of fresh produce samples for microbiological indicator enumeration. | several methods have been described to prepare fresh produce samples for microbiological analysis, each with its own advantages and disadvantages. the aim of this study was to compare the performance of a novel combined rinse and membrane filtration method to two alternative sample preparation methods for the quantification of indicator microorganisms from fresh produce. decontaminated cantaloupe melons and jalapeño peppers were surface inoculated with a cocktail containing 10(6) cfu/ml escheric ... | 2015 | 25719876 |
| effect of apically separated rotary instruments on bacterial leakage of obturated root canals using resilon. | the purpose of this study was to determine the effect of a separated rotary instrument on the time required for bacterial penetration of obturated root canals using resilon. eighty-four extracted human maxillary premolars with single canals were used in this study. group 1 consisted of teeth obturated with gutta-percha and ah-26 sealer (n=15); group 2 consisted of teeth obturated similarly to group 1 but without a separated file; group 3 consisted of teeth obturated with resilon and epiphany sea ... | 2015 | 25928974 |
| genotypic variations of virulent genes in enterococcus faecium and enterococcus faecalis isolated from three hospitals in malaysia. | the genus enterococcus is of increasing significance as a cause of nosocomial infections, and this trend is exacerbated by the development of antibiotic resistance. | 2015 | 25923096 |