Publications

functional evolution of a morphogenetic gradient.	bone morphogenetic proteins (bmps) pattern the dorsal-ventral axis of bilaterian embryos; however, their roles in the evolution of body plan are largely unknown. we examined their functional evolution in fly embryos. bmp signaling specifies two extraembryonic tissues, the serosa and amnion, in basal-branching flies such as megaselia abdita, but only one, the amnioserosa, in drosophila melanogaster. the bmp signaling dynamics are similar in both species until the beginning of gastrulation, when b ...	2016	28005004
control of vector-borne human parasitic diseases.		2016	28090535
spatiotemporal frameworks for infectious disease diffusion and epidemiology.	emerging infectious diseases, and the resurgence of previously controlled infectious disease (e.g., malaria, tuberculosis), are a major focus for public health concern, as well as providing challenges for establishing aetiology and transmission. [...].	2016	27999420
dengue fever-associated maculopathy and panuveitis in australia.	purpose. to describe a case of dengue fever-associated maculopathy and panuveitis to raise awareness of these ophthalmic complications of dengue in australia in the light of recent increasing numbers of outbreaks from equatorial through to tropical australia. case report. a 37-year-old caucasian australian male returning from cambodia presented with a bilateral dengue fever-associated maculopathy with left panuveitis diagnosed clinically and haematologically. automated perimetry revealed bilater ...	2016	28078150
cloning, expression, and characterization of prophenoloxidases from asian corn borer, ostrinia furnacalis (gunée).	insect phenoloxidase (po) belongs to the type 3 copper protein family and possesses oxidoreductase activities. po is typically synthesized as a zymogen called prophenoloxidase (ppo) and requires the proteolytic activation to function. we here cloned full-length cdna for 3 previously unidentified ppos, which we named ofppo1a, ofppo1b, and ofppo3, from asian corn borer, ostrinia furnacalis (gunée), in addition to the previously known ofppo2. these conceptual ppos and ofppo2 all contain two common ...	2016	28078308
data documenting the potential distribution of aedes aegypti in the center of veracruz, mexico.	the data presented in this article are related to the research article entitled "establishment of aedes aegypti (l.) in mountainous regions in mexico: increasing number of population at risk of mosquito-borne disease and future climate conditions" (m. equihua, s. ibáñez-bernal, g. benítez, i. estrada-contreras, c.a. sandoval-ruiz, f.s. mendoza-palmero, 2016) [1]. this article provides presence records in shapefile format used to generate maps of potential distribution of aedes aegypti with diffe ...	2016	28054003
economic report on the cost of dengue fever in vietnam: case of a provincial hospital.	dengue is a mosquito-borne viral illness with the world's fastest rate of infection. in 2014, vietnam had recorded 43,000 cases in 53 provinces, with 28 deaths.	2016	28031723
attempts to image the early inflammatory response during infection with the lymphatic filarial nematode brugia pahangi in a mouse model.	helminth parasites remain a major constraint upon human health and well-being in many parts of the world. treatment of these infections relies upon a very small number of therapeutics, most of which were originally developed for use in animal health. a lack of high throughput screening systems, together with limitations of available animal models, has restricted the development of novel chemotherapeutics. this is particularly so for filarial nematodes, which are long-lived parasites with a compl ...	2016	27992545
rna interference as a gene silencing tool to control tuta absoluta in tomato (solanum lycopersicum).	rna interference (rnai), a gene-silencing mechanism that involves providing double-stranded rna molecules that match a specific target gene sequence, is now widely used in functional genetic studies. the potential application of rnai-mediated control of agricultural insect pests has rapidly become evident. the production of transgenic plants expressing dsrna molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resultin ...	2016	27994959
variations on a theme: antennal lobe architecture across coleoptera.	beetles comprise about 400,000 described species, nearly one third of all known animal species. the enormous success of the order coleoptera is reflected by a rich diversity of lifestyles, behaviors, morphological, and physiological adaptions. all these evolutionary adaptions that have been driven by a variety of parameters over the last about 300 million years, make the coleoptera an ideal field to study the evolution of the brain on the interface between the basic bauplan of the insect brain a ...	2016	27973569
identification of development-related genes in the ovaries of adult harmonia axyridis (pallas) lady beetles using a time- series analysis by rna-seq.	adults of the lady beetle species harmonia axyridis (pallas) are bred artificially en masse for classic biological control, which requires egg-laying by the h. axyridis ovary. development-related genes may impact the growth of the h. axyridis adult ovary but have not been reported. here, we used integrative time-series rna-seq analysis of the ovary in h. axyridis adults to detect development-related genes. a total of 28,558 unigenes were functionally annotated using seven types of databases to o ...	2016	27966611
how do households respond to unreliable water supplies? a systematic review.	although the millennium development goal (mdg) target for drinking water was met, in many developing countries water supplies are unreliable. this paper reviews how households in developing countries cope with unreliable water supplies, including coping costs, the distribution of coping costs across socio-economic groups, and effectiveness of coping strategies in meeting household water needs. structured searches were conducted in peer-reviewed and grey literature in electronic databases and sea ...	2016	27941695
aphids transform and detoxify the mycotoxin deoxynivalenol via a type ii biotransformation mechanism yet unknown in animals.	biotransformation of mycotoxins in animals comprises phase i and phase ii metabolisation reactions. for the trichothecene deoxynivalenol (don), several phase ii biotransformation reactions have been described resulting in don-glutathiones, don-glucuronides and don-sulfates made by glutathione-s-transferases, uridine-diphosphoglucuronyl transferases and sulfotransferases, respectively. these metabolites can be easily excreted and are less toxic than their free compounds. here, we demonstrate for ...	2016	27929076
consecutive large dengue outbreaks in taiwan in 2014-2015.		2016	27924810
transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the australian crayfish, cherax quadricarinatus.	the australian redclaw crayfish (cherax quadricarinatus) has recently received attention as an emerging candidate for sustainable aquaculture production in australia and worldwide. more importantly, c. quadricarinatus serves as a good model organism for the commercially important group of decapod crustaceans as it is distributed worldwide, easy to maintain in the laboratory and its reproductive cycle has been well documented. in order to better understand the key reproduction and development reg ...	2016	27924858
retrospective analysis of prognostic factors in dengue infected patients with intracranial bleed.	dengue is one of the most common mosquito-transmitted arboviral disease of tropical and a few subtropical areas in the world. it is estimated that approximately 100 million cases occur per year and approximately 2.5 billion people are at risk of developing dengue infection. hemorrhagic complications causing encephalopathy are quite rare but fatal consequences of this deadly disease. this study was conducted to discuss the prognostic factors in the management of intracranial hemorrhage in dengue ...	2016	28031986
control of aedes aegypti breeding: a novel intervention for prevention and control of dengue in an endemic zone of delhi, india.	the study is based on hypothesis that whether continuous entomological surveillance of ae. aegypti and simultaneous appropriate interventions in key containers during non-transmission (december-may) months would have any impact on breeding of aedes and dengue cases during the following transmission months (june-november). the impact of the surveillance and intervention measures undertaken during non-transmission months were assessed by entomological indicators namely container index (ci), house ...	2016	27918577
beyond traditional antimicrobials: a caenorhabditis elegans model for discovery of novel anti-infectives.	the spread of antibiotic resistance amongst bacterial pathogens has led to an urgent need for new antimicrobial compounds with novel modes of action that minimize the potential for drug resistance. to date, the development of new antimicrobial drugs is still lagging far behind the rising demand, partly owing to the absence of an effective screening platform. over the last decade, the nematode caenorhabditis elegans has been incorporated as a whole animal screening platform for antimicrobials. th ...	2016	27994583
susceptibility of phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host.	a pleiotropic response to the calpain inhibitor mdl28170 was detected in the tomato parasite phytomonas serpens. ultrastructural studies revealed that mdl28170 caused mitochondrial swelling, shortening of flagellum and disruption of trans golgi network. this effect was correlated to the inhibition in processing of cruzipain-like molecules, which presented an increase in expression paralleled by decreased proteolytic activity. concomitantly, a calcium-dependent cysteine peptidase was detected in ...	2016	27925020
size, sounds and sex: interactions between body size and harmonic convergence signals determine mating success in aedes aegypti.	several new mosquito control strategies will involve the release of laboratory reared males which will be required to compete with wild males for mates. currently, the determinants of male mating success remain unclear. the presence of convergence between male and female harmonic flight tone frequencies during a mating attempt have been found to increase male mating success in the yellow fever mosquito, aedes aegypti. size has also been implicated as a factor in male mating success. here, we inv ...	2016	27906074
clinical profiles of dengue infection during an outbreak in northern india.	introduction. dengue fever is an arboviral disease, which is transmitted by mosquito vector and presents as varied clinical spectrum of dengue fever (df), dengue hemorrhagic fever (dhf), dengue shock syndrome (dss), and expanded dengue syndrome (eds) with atypical presentations, thus posing a diagnostic dilemma. unless we are aware of these presentations, diagnosis as well as early initiation of treatment becomes difficult. we studied the various clinical presentations of dengue infection during ...	2016	28025597
path of infectious diseases in brazil in the last 50 years: an ongoing challenge.	in this article, we comment on the main features of infectious diseases in brazil in the last 50 years, highlighting how much of this path revista de saúde pública could portray. from 1967 to 2016, 1,335 articles focusing on infectious diseases were published in revista de saúde pública. although the proportion of articles on the topic have decreased from about 50.0% to 15.0%, its notability remained and reflected the growing complexity of the research required for its control. it is noteworthy ...	2016	28099652
contribution of oswaldo paulo forattini to public health: analysis of scientific production.	to analyze the main characteristics of the scientific production of oswaldo paulo forattini, researcher and, for 40 years, editor of revista de saúde pública.	2016	28099651
small rnas from bemisia tabaci are transferred to solanum lycopersicum phloem during feeding.	the phloem-feeding whitefly bemisia tabaci is a serious pest to a broad range of host plants, including many economically important crops such as tomato. these insects serve as a vector for various devastating plant viruses. it is known that whiteflies are capable of manipulating host-defense responses, potentially mediated by effector molecules in the whitefly saliva. we hypothesized that, beside putative effector proteins, small rnas (srna) are delivered by b. tabaci into the phloem, where the ...	2016	27933079
the worldwide spread of the tiger mosquito as revealed by mitogenome haplogroup diversity.	in the last 40 years, the asian tiger mosquito aedes albopictus, indigenous to east asia, has colonized every continent except antarctica. its spread is a major public health concern, given that this species is a competent vector for numerous arboviruses, including those causing dengue, chikungunya, west nile, and the recently emerged zika fever. to acquire more information on the ancestral source(s) of adventive populations and the overall diffusion process from its native range, we analyzed th ...	2016	27933090
detection of wolbachia in aedes albopictus and their effects on chikungunya virus.	wolbachia-based vector control strategies have been proposed as a means to augment the currently existing measures for controlling dengue and chikungunya vectors. prior to utilizing wolbachia as a novel vector control strategy, it is crucial to understand the wolbachia-mosquito interactions. in this study, field surveys were conducted to screen for the infection status of wolbachia in field-collected aedes albopictus the effects of wolbachia in its native host toward the replication and dissemin ...	2016	27920393
evaluation of the larvicidal efficacy of five indigenous weeds against an indian strain of dengue vector, aedes aegypti l. (diptera: culicidae).	background and objectives. aedes aegypti, dengue fever mosquito, is primarily associated with the transmission of dengue and chikungunya in tropical and subtropical regions of the world. the present investigations were carried out to assess the larvicidal efficiency of five indigenous weeds against ae. aegypti. methods. the 1,000 ppm hexane and ethanol extracts prepared from the leaves and stem of five plants (achyranthes aspera, cassia occidentalis, catharanthus roseus, lantana camara, and xant ...	2016	26941996
horizontally transferred genetic elements in the tsetse fly genome: an alignment-free clustering approach using batch learning self-organising map (blsom).	tsetse flies (glossina spp.) are the primary vectors of trypanosomes, which can cause human and animal african trypanosomiasis in sub-saharan african countries. the objective of this study was to explore the genome of glossina morsitans morsitans for evidence of horizontal gene transfer (hgt) from microorganisms. we employed an alignment-free clustering method, that is, batch learning self-organising map (blsom), in which sequence fragments are clustered based on the similarity of oligonucleotid ...	2016	28074180
seasonal differences in density but similar competitive impact of aedes albopictus (skuse) on aedes aegypti (l.) in rio de janeiro, brazil.	previous studies have shown that the negative effects of density of ae. albopictus on ae. aegypti exceed those of ae. aegypti on ae. albopictus for population growth, adult size, survivorship, and developmental rate. this competitive superiority has been invoked to explain the displacement of ae. aegypti by ae. albopictus in the southeastern usa. in brazil, these species coexist in many vegetated suburban and rural areas. we investigated a related, but less-well-studied question: do effects of a ...	2016	27322537
bioinformatic characterization of mosquito viromes within the eastern united states and puerto rico: discovery of novel viruses.	mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many rna viruses. the vast majority of all viruses are thought to be undiscovered. accordingly, recent studies have shown that viruses discovered in insects are very divergent from known pathogens and that many of them lack appropriate reference sequences in the public databases. given that the majority of viruses are likely still undiscovered, environ mental sampling stands to provide much needed re ...	2016	27346944
phylosymbiosis: relationships and functional effects of microbial communities across host evolutionary history.	phylosymbiosis was recently proposed to describe the eco-evolutionary pattern, whereby the ecological relatedness of host-associated microbial communities parallels the phylogeny of related host species. here, we test the prevalence of phylosymbiosis and its functional significance under highly controlled conditions by characterizing the microbiota of 24 animal species from four different groups (peromyscus deer mice, drosophila flies, mosquitoes, and nasonia wasps), and we reevaluate the phylos ...	2016	27861590
synergistic and compensatory effects of two point mutations conferring target-site resistance to fipronil in the insect gaba receptor rdl.	insecticide resistance can arise from a variety of mechanisms, including changes to the target site, but is often associated with substantial fitness costs to insects. here we describe two resistance-associated target-site mutations that have synergistic and compensatory effects that combine to produce high and persistent levels of resistance to fipronil, an insecticide targeting on γ-aminobytyric acid (gaba) receptors. in nilaparvata lugens, a major pest of rice crops in many parts of asia, we ...	2016	27557781
the p450 cyp6z1 confers carbamate/pyrethroid cross-resistance in a major african malaria vector beside a novel carbamate-insensitive n485i acetylcholinesterase-1 mutation.	carbamates are increasingly used for vector control notably in areas with pyrethroid resistance. however, a cross-resistance between these insecticides in major malaria vectors such as anopheles funestus could severely limit available resistance management options. unfortunately, the molecular basis of such cross-resistance remains uncharacterized in an. funestus, preventing effective resistance management. here, using a genomewide transcription profiling, we revealed that metabolic resistance t ...	2016	27135886
crowdsourcing vector surveillance: using community knowledge and experiences to predict densities and distribution of outdoor-biting mosquitoes in rural tanzania.	lack of reliable techniques for large-scale monitoring of disease-transmitting mosquitoes is a major public health challenge, especially where advanced geo-information systems are not regularly applicable. we tested an innovative crowd-sourcing approach, which relies simply on knowledge and experiences of residents to rapidly predict areas where disease-transmitting mosquitoes are most abundant. guided by community-based resource persons, we mapped boundaries and major physical features in three ...	2016	27253869
silencing of the olfactory co-receptor gene in dendroctonus armandi leads to eag response declining to major host volatiles.	in this study, a polymerase chain reaction (pcr) based on homology genes of orco was utilized to identify darmorco, which is essential for olfaction in d. armandi. the results showed that darmorco shares significant sequence homology with orco proteins had known in other insects. quantitative real-time pcr (qrt-pcr) analysis suggested that darmorco was abundantly expressed in adult d. armandi; by contrast, darmorco showed trace amounts of expression level in other stages. of different tissues, d ...	2016	26979566
contact bioassays with phenoxybenzyl and tetrafluorobenzyl pyrethroids against target-site and metabolic resistant mosquitoes.	mosquito strains that exhibit increased tolerance to the chemical class of compounds with a sodium channel modulator mode of action (pyrethroids and pyrethrins) are typically described as "pyrethroid resistant". resistance to pyrethroids is an increasingly important challenge in the control of mosquito-borne diseases, such as malaria or dengue, because one of the main interventions (the distribution of large numbers of long-lasting insecticide-treated bed nets) currently relies entirely on long- ...	2016	26930058
combining synthetic human odours and low-cost electrocuting grids to attract and kill outdoor-biting mosquitoes: field and semi-field evaluation of an improved mosquito landing box.	on-going malaria transmission is increasingly mediated by outdoor-biting vectors, especially where indoor insecticidal interventions such as long-lasting insecticide treated nets (llins) are widespread. often, the vectors are also physiologically resistant to insecticides, presenting major obstacles for elimination. we tested a combination of electrocuting grids with synthetic odours as an alternative killing mechanism against outdoor-biting mosquitoes.	2016	26789733
the influence of larval competition on brazilian wolbachia-infected aedes aegypti mosquitoes.	with field releases starting in brazil, particular interest must be given to understanding how the endosymbiotic bacterium wolbachia pipientis affects aedes aegypti mosquitoes with a brazilian genetic background. currently, there is limited information on how the bacterium affects phenotypic traits such as larval development rate, metabolic reserves and morphometric parameters in ae. aegypti. here, we analyze for the first time, the effect of wolbachia on these key phenotypes and consider how th ...	2016	27183820
determinants of host feeding success by anopheles farauti.	the proportion of blood meals that mosquitoes take from a host species is a function of the interplay of extrinsic (abundance and location of potential hosts) and intrinsic (innate preference) factors. a mark-release-recapture experiment addressed whether host preference in a population of anopheles farauti was uniform or if there were anthropophilic and zoophilic subpopulations. the corresponding fitness associated with selecting different hosts for blood meals was compared by measuring fecundi ...	2016	26964528
vector-control response in a post-flood disaster setting, honiara, solomon islands, 2014.	the close quartering and exposed living conditions in evacuation centres and the potential increase in vector density after flooding in solomon islands resulted in an increased risk of exposure for the occupants to vectorborne diseases.	2016	27757255
functional and biochemical characterization of three recombinant human glucose-6-phosphate dehydrogenase mutants: zacatecas, vanua-lava and viangchan.	glucose-6-phosphate dehydrogenase (g6pd) deficiency in humans causes severe disease, varying from mostly asymptomatic individuals to patients showing neonatal jaundice, acute hemolysis episodes or chronic nonspherocytic hemolytic anemia. in order to understand the effect of the mutations in g6pd gene function and its relation with g6pd deficiency severity, we report the construction, cloning and expression as well as the detailed kinetic and stability characterization of three purified clinical ...	2016	27213370
male tarsi specific odorant-binding proteins in the diving beetle cybister japonicus sharp.	odorant binding proteins (obps) play critical roles in chemical communication of insects, as they recognize and transport environmental chemical signals to receptors. the diving beetle cybister japonicus sharp shows a remarkable sexual dimorphism. the foreleg tarsi of males are equipped with large suction cups, believed to help holding the female during underwater courtship and mating. here, we identified two obps highly and specifically expressed in male tarsi, suggesting important functions of ...	2016	27545810
the retinoid x receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.	in arthropods, retinoid x receptor (rxr) is a highly conserved nuclear hormone receptor. by forming a heterodimeric complex with the ecdysone receptor (ecr), rxr is known to be vital importance for various physiological processes. however, in comparison to ecr, the rxr signaling pathway and its roles in crustacean reproduction are poorly understood. in the present study, the rxr mrna was detected in the ovarian follicular cells of mud crab scylla paramamosain (sprxr) and during ovarian maturatio ...	2016	27009370
transcriptomic data from panarthropods shed new light on the evolution of insulator binding proteins in insects : insect insulator proteins.	body plan development in multi-cellular organisms is largely determined by homeotic genes. expression of homeotic genes, in turn, is partially regulated by insulator binding proteins (ibps). while only a few enhancer blocking ibps have been identified in vertebrates, the common fruit fly drosophila melanogaster harbors at least twelve different enhancer blocking ibps. we screened recently compiled insect transcriptomes from the 1kite project and genomic and transcriptomic data from public databa ...	2016	27809783
comparative profiling of micrornas in the winged and wingless english grain aphid, sitobion avenae (f.) (homoptera: aphididae).	micrornas (mirnas) are short single-stranded non-coding rnas that regulate gene expression, particularly during development. in this study, 345 mirnas were identified from the english green aphid, sitobion avenae (f.), of which 168 were conserved and 177 were s. avenae-specific. quantitative comparison of mirna expression levels indicated that 16 and 12 mirnas were significantly up-regulated in winged and wingless s. avenae small rna libraries, respectively. differential expression of these mirn ...	2016	27762301
identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean talitrus saltator.	talitrus saltator is an amphipod crustacean that inhabits the supralittoral zone on sandy beaches in the northeast atlantic and mediterranean. t. saltator exhibits endogenous locomotor activity rhythms and time-compensated sun and moon orientation, both of which necessitate at least one chronometric mechanism. whilst their behaviour is well studied, currently there are no descriptions of the underlying molecular components of a biological clock in this animal, and very few in other crustacean sp ...	2016	27761341
computational characterization of iron metabolism in the tsetse disease vector, glossina morsitans: ire stem-loops.	iron metabolism and regulation is an indispensable part of species survival, most importantly for blood feeding insects. iron regulatory proteins are central regulators of iron homeostasis, whose binding to iron response element (ire) stem-loop structures within the utrs of genes regulate expression at the post-transcriptional level. despite the extensive literature on the mechanism of iron regulation in human, less attention has been given to insect and more specifically the blood feeding insec ...	2016	27503259
gene family evolution reflects adaptation to soil environmental stressors in the genome of the collembolan orchesella cincta.	collembola (springtails) are detritivorous hexapods that inhabit the soil and its litter layer. the ecology of the springtail orchesella cincta is extensively studied in the context of adaptation to anthropogenically disturbed areas. here, we present a draft genome of an o. cincta reference strain with an estimated size of 286.8 mbp, containing 20,249 genes. in total, 446 gene families are expanded and 1,169 gene families evolved specific to this lineage. besides these gene families involved in ...	2016	27289101
composition and genomic organization of arthropod hox clusters.	the ancestral arthropod is believed to have had a clustered arrangement of ten hox genes. within arthropods, hox gene mutations result in transformation of segment identities. despite the fact that variation in segment number/character was common in the diversification of arthropods, few examples of hox gene gains/losses have been correlated with morphological evolution. furthermore, a full appreciation of the variation in the genomic arrangement of hox genes in extant arthropods has not been re ...	2016	27168931
biological screening of selected pacific northwest forest plants using the brine shrimp (artemia salina) toxicity bioassay.	the brine shrimp (artemia salina) bioassay was used to screen 211 methanol extracts from 128 species of pacific northwest plants in search of general cytotoxic activity. strong toxicity (lc50 < 100 µg/ml) was found for 17 extracts from 13 species, with highest activity observed for angelica arguta roots at <10 µg/ml. notably, four species of cedar trees and one of juniper in the family cupressaceae dominated this group with lc50 for heartwood extracts ranging from 15 to 89 µg/ml. moderate toxici ...	2016	27186474
comparative pharmacological characterization of d1-like dopamine receptors from anopheles gambiae, aedes aegypti and culex quinquefasciatus suggests pleiotropic signaling in mosquito vector lineages.	small molecule antagonists of mosquito dopamine receptors (dars) are under investigation as a new class of vector-selective insecticides. antagonists that inhibit the d1-like dars aadop2 and cqdop2 from the mosquitoes aedes aegypti l. and culex quinquefasciatus say, respectively, also cause larval mortality in bioassays. here, we report on the orthologous dar, agdop2, from the malaria mosquito anopheles gambiae giles that was cloned and pharmacologically characterized in hek293 cells. larval bio ...	2016	27048546
comparative study of hippo pathway genes in cellular conveyor belts of a ctenophore and a cnidarian.	the hippo pathway regulates growth rate and organ size in fly and mouse, notably through control of cell proliferation. molecular interactions at the heart of this pathway are known to have originated in the unicellular ancestry of metazoans. they notably involve a cascade of phosphorylations triggered by the kinase hippo, with subsequent nuclear to cytoplasmic shift of yorkie localisation, preventing its binding to the transcription factor scalloped, thereby silencing proliferation genes. there ...	2016	26900447
duplication and diversification of dipteran argonaute genes, and the evolutionary divergence of piwi and aubergine.	genetic studies of drosophila melanogaster have provided a paradigm for rna interference (rnai) in arthropods, in which the microrna and antiviral pathways are each mediated by a single argonaute (ago1 and ago2) and germline suppression of transposable elements is mediated by a trio of piwi-subfamily argonaute proteins (ago3, aub, and piwi). without a suitable evolutionary context, deviations from this can be interpreted as derived or idiosyncratic. here we analyze the evolution of argonaute gen ...	2016	26868596
multiple functions of na/k-atpase in dopamine-induced salivation of the blacklegged tick, ixodes scapularis.	control of salivary secretion in ticks involves autocrine dopamine activating two dopamine receptors: d1 and invertebrate-specific d1-like dopamine receptors. in this study, we investigated na/k-atpase as an important component of the secretory process. immunoreactivity for na/k-atpase revealed basal infolding of lamellate cells in type-i, abluminal interstitial (epithelial) cells in type-ii, and labyrinth-like infolding structures opening towards the lumen in type-iii acini. ouabain (10 μmol l( ...	2016	26861075
mechanisms of sex determination and transmission ratio distortion in aedes aegypti.	more effective mosquito control strategies are urgently required due to the increasing prevalence of insecticide resistance. the sterile insect technique (sit) and the release of insects carrying a dominant lethal allele (ridl) are two proposed methods for environmentally-friendly, species-targeted population control. these methods may be more suitable for developing countries if producers reduce the cost of rearing insects. the cost of control programs could be reduced by producing all-male mos ...	2016	26818000
ligand selectivity in tachykinin and natalisin neuropeptidergic systems of the honey bee parasitic mite varroa destructor.	the varroa mite, varroa destructor, is a devastating ectoparasite of the honey bees apis mellifera and a. cerana. control of these mites in beehives is a challenge in part due to the lack of toxic agents that are specific to mites and not to the host honey bee. in searching for a specific toxic target of varroa mites, we investigated two closely related neuropeptidergic systems, tachykinin-related peptide (trp) and natalisin (ntl), and their respective receptors. honey bees lack both ntl and the ...	2016	26817786
international air travel to ohio, usa, and the impact on malaria, influenza, and hepatitis a.	the state of ohio led the united states in measles in 2014, ostensibly related to international air travel (iat), and ranked lower than 43 other states in infectious disease outbreak preparedness. we conducted a retrospective cohort study using surveillance data of the total ohio population of 11 million from 2010 through 2014 with a nested case control of air travelers to determine the risk of malaria, seasonal influenza hospitalizations (ih), and hepatitis a (ha) disease related to internation ...	2016	27123365
prediction of dengue outbreaks based on disease surveillance and meteorological data.	research is needed to create early warnings of dengue outbreaks to inform stakeholders and control the disease. this analysis composes of a comparative set of prediction models including only meteorological variables; only lag variables of disease surveillance; as well as combinations of meteorological and lag disease surveillance variables. generalized linear regression models were used to fit relationships between the predictor variables and the dengue surveillance data as outcome variable on ...	2016	27031524
evocation to the dr. carlos j. finlay barres on the centennial of his death.	about the editorial of the professor guillermo llanos: "carlos j. finlay: the forgotten pasteur of america", a hundred years after his death and through a documental review, a summary of the life and work of this great man of science was conducted. finlay was a notable figure of the american medicine, he conceived a new infection way able to explain the propagation of the yellow fever, and added the possibility of their scientific confirmation by an experimental method. for all the above-mention ...	2016	27226667
tor pathway-mediated juvenile hormone synthesis regulates nutrient-dependent female reproduction in nilaparvata lugens (stål).	the "target of rapamycin" (tor) nutritional signaling pathway and juvenile hormone (jh) regulation of vitellogenesis has been known for a long time. however, the interplay between these two pathways regulating vitellogenin (vg) expression remains obscure. here, we first demonstrated the key role of amino acids (aas) in activation of vg synthesis and egg development in nilaparvata lugens using chemically defined artificial diets. aas induced the expression of tor and s6k (s6 kinase), whereas rnai ...	2016	27043527
virtual screening of plant volatile compounds reveals a high affinity of hylamorpha elegans (coleoptera: scarabaeidae) odorant-binding proteins for sesquiterpenes from its native host.	hylamorpha elegans(burmeister) is a native chilean scarab beetle considered to be a relevant agricultural pest to pasture and cereal and small fruit crops. because of their cryptic habits, control with conventional methods is difficult; therefore, alternative and environmentally friendly control strategies are highly desirable. the study of proteins that participate in the recognition of odorants, such as odorant-binding proteins (obps), offers interesting opportunities to identify new compounds ...	2016	27012867
identification and characterization of two "sensory neuron membrane proteins" (snmps) of the desert locust, schistocerca gregaria (orthoptera: acrididae).	pheromone-responsive neurons of insects not only require specific receptors but in addition several auxiliary components, including the "sensory neuron membrane protein," snmp. accordingly, snmp is considered as a marker for neurons responding to pheromones. for the desert locust schistocerca gregaria, it is known that the behavior, including aggregation behavior and courtship inhibition, is largely controlled by pheromones. however, little is known about pheromones, their receptors, and the phe ...	2016	27012870
correction: how diverse detrital environments influence nutrient stoichiometry between males and females of the co-occurring container mosquitoes aedes albopictus, ae. aegypti, and culex quinquefasciatus.		2016	27007775
genome-wide analysis of ionotropic receptors provides insight into their evolution in heliconius butterflies.	in a world of chemical cues, smell and taste are essential senses for survival. here we focused on heliconius, a diverse group of butterflies that exhibit variation in pre- and post-zygotic isolation and chemically-mediated behaviors across their phylogeny. our study examined the ionotropic receptors, a recently discovered class of receptors that are some of the most ancient chemical receptors.	2016	27004525
risk associated with the release of wolbachia-infected aedes aegypti mosquitoes into the environment in an effort to control dengue.	in an effort to eliminate dengue, a successful technology was developed with the stable introduction of the obligate intracellular bacteria wolbachia pipientis into the mosquito aedes aegypti to reduce its ability to transmit dengue fever due to life shortening and inhibition of viral replication effects. an analysis of risk was required before considering release of the modified mosquito into the environment.	2016	27047911
minocycline as a re-purposed anti-wolbachia macrofilaricide: superiority compared with doxycycline regimens in a murine infection model of human lymphatic filariasis.	lymphatic filariasis and onchocerciasis are parasitic helminth diseases, which cause severe morbidities such as elephantiasis, skin disease and blindness, presenting a major public health burden in endemic communities. the anti-wolbachia consortium (a·wol: http://www.a-wol.com/) has identified a number of registered antibiotics that target the endosymbiotic bacterium, wolbachia, delivering macrofilaricidal activity. here we use pharmacokinetics/pharmacodynamics (pk/pd) analysis to rationally dev ...	2016	26996237
is dengue vector control deficient in effectiveness or evidence?: systematic review and meta-analysis.	although a vaccine could be available as early as 2016, vector control remains the primary approach used to prevent dengue, the most common and widespread arbovirus of humans worldwide. we reviewed the evidence for effectiveness of vector control methods in reducing its transmission.	2016	26986468
spectrum of maternofetal outcomes during dengue infection in pregnancy: an insight.	dengue is a vector transmitted viral infection; tropical and subtropical countries see outbreaks of dengue each year. there is a paucity of literature on effects of dengue infection on pregnancy outcome and this prompted us to undertake a study for better understanding of pregnancy implications with dengue infection. pregnant women admitted during the seasonal outbreak of dengue between september 2015 and october 2015 were studied and maternal and fetal outcomes in sixteen ns1ag positive women w ...	2016	27069349
the hitchhiker's guide to europe: the infection dynamics of an ongoing wolbachia invasion and mitochondrial selective sweep in rhagoletis cerasi.	wolbachia is a maternally inherited and ubiquitous endosymbiont of insects. it can hijack host reproduction by manipulations such as cytoplasmic incompatibility (ci) to enhance vertical transmission. horizontal transmission of wolbachia can also result in the colonization of new mitochondrial lineages. in this study, we present a 15-year-long survey of wolbachia in the cherry fruit fly rhagoletis cerasi across europe and the spatiotemporal distribution of two prevalent strains, wcer1 and wcer2, ...	2016	26846713
sfdredd, a novel initiator caspase possessing activity on effector caspase substrates in spodoptera frugiperda.	sf9, a cell line derived from spodoptera frugiperda, is an ideal model organism for studying insect apoptosis. the first notable study that attempted to identify the apoptotic pathway in sf9 was performed in 1997 and included the discovery of sf-caspase-1, an effector caspase of sf9. however, it was not until 2013 that the first initiator caspase in sf9, sfdronc, was discovered, and the apoptotic pathway in sf9 became clearer. in this study, we report another caspase of sf9, sfdredd. sfdredd is ...	2016	26977926
identification of a tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.	the saliva of tsetse flies contains a cocktail of bioactive molecules inducing specific antibody responses in hosts exposed to bites. we have previously shown that an indirect-elisa test using whole salivary extracts from glossina morsitans submorsitans was able to discriminate between (i) cattle from tsetse infested and tsetse free areas and (ii) animals experimentally exposed to low or high numbers of tsetse flies. in the present study, our aim was to identify specific salivary synthetic pepti ...	2016	26979518
ambivalent outcomes of cell apoptosis: a barrier or blessing in malaria progression.	the life cycle of plasmodium in two evolutionary distant hosts, mosquito, and human, is a complex process. it is regulated at various stages of developments by a number of diverged mechanisms that ultimately determine the outcome of the disease. during the development processes, plasmodium invades a variety of cells in two hosts. the invaded cells tend to undergo apoptosis and are subsequently removed from the system. this process also eliminates numerous parasites along with these apoptotic cel ...	2016	27014225
factors associated with correct and consistent insecticide treated curtain use in iquitos, peru.	dengue is an arthropod-borne virus of great public health importance, and control of its mosquito vectors is currently the only available method for prevention. previous research has suggested that insecticide treated curtains (itcs) can lower dengue vector infestations in houses. this observational study investigated individual and household-level socio-demographic factors associated with correct and consistent use of itcs in iquitos, peru. a baseline knowledge, attitudes, and practices (kap) s ...	2016	26967157
integrating transgenic vector manipulation with clinical interventions to manage vector-borne diseases.	many vector-borne diseases lack effective vaccines and medications, and the limitations of traditional vector control have inspired novel approaches based on using genetic engineering to manipulate vector populations and thereby reduce transmission. yet both the short- and long-term epidemiological effects of these transgenic strategies are highly uncertain. if neither vaccines, medications, nor transgenic strategies can by themselves suffice for managing vector-borne diseases, integrating these ...	2016	26962871
rnai trigger delivery into anopheles gambiae pupae.	rna interference (rnai), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory for functional genomic studies. the ability to knockdown individual genes selectively via this reverse genetic technique has allowed many researchers to rapidly uncover the biological roles of numerous genes within many organisms, by evaluation of loss-of-function phenotypes. in the major human malaria vector anopheles gambiae, the predominant me ...	2016	27023367
erratum to: co-distribution and co-infection of chikungunya and dengue viruses.		2016	27129475
rhodnius prolixus and r. robustus (hemiptera: reduviidae) nymphs show different locomotor patterns on an automated recording system.	circadian rhythms of triatomines, vectors of the etiological agent trypanosoma cruzi responsible for chagas disease, have been extensively studied in adults of the two most epidemiologically relevant vector species, rhodnius prolixus and triatoma infestans. however, little attention has been dedicated to the activity patterns in earlier developmental stages, even though triatomine nymphs are equally capable of transmitting t. cruzi to humans. because circadian rhythms may differ even between clo ...	2016	27121502
de novo adult transcriptomes of two european brittle stars: spotlight on opsin-based photoreception.	next generation sequencing (ngs) technology allows to obtain a deeper and more complete view of transcriptomes. for non-model or emerging model marine organisms, ngs technologies offer a great opportunity for rapid access to genetic information. in this study, paired-end illumina hiseqtm technology has been employed to analyse transcriptomes from the arm tissues of two european brittle star species, amphiura filiformis and ophiopsila aranea. about 48 million illumina reads were generated and 136 ...	2016	27119739
excretion of dengue virus rna by aedes aegypti allows non-destructive monitoring of viral dissemination in individual mosquitoes.	successful transmission of a vector-borne pathogen relies on a complex life cycle in the arthropod vector that requires initial infection of the digestive tract followed by systemic viral dissemination. the time interval between acquisition and subsequent transmission of the pathogen, called the extrinsic incubation period, is one of the most influential parameters of vector-borne pathogen transmission. however, the dynamic nature of this process is often ignored because vector competence assays ...	2016	27117953
droplet characterization and penetration of an ultra-low volume mosquito adulticide spray targeting the asian tiger mosquito, aedes albopictus, within urban and suburban environments of northeastern usa.	adult control of aedes albopictus via ultra-low volume is difficult because this species occurs primarily in peridomestic habitats where obstacles such as buildings and vegetation can disrupt spray plumes and droplet dispersion. we determined droplet penetration and characterization of a pyrethroid adulticide applied from the ground at mid (46.77 ml/ha) and maximum (93.53 ml/ha) label rates within cryptic habitats of urban and suburban environments. droplets were collected from all habitats, wit ...	2016	27116103
knowledge, attitudes, and practices of florida physicians regarding dengue before and after an educational intervention.	failure to recognize and appropriately manage dengue early in the clinical course may result in late initiation of supportive treatment for severe disease. in florida, travel-related and autochthonous dengue occur and are likely under-recognized. the objective of this study was to evaluate physician knowledge of dengue and its management before and after an educational intervention in florida.	2016	27112138
pfsmad4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster pinctada fucata.	mollusca is the second largest phylum in nature. the shell of molluscs is a remarkable example of a natural composite biomaterial. biomineralization and how it affects mollusks is a popular research topic. the bmp-2 signaling pathway plays a canonical role in biomineralization. smad4 is an intracellular transmitter in the bmp signaling pathway in mammals, and some genomic data show smad4's involvement in bmp signaling in invertebrates, but whether smad4 plays a conservative role in pearl oyster, ...	2016	27113217
clinical profile of dengue fever in children: a study from southern odisha, india.	background. in india, dengue epidemics are becoming more frequent (who, 2008). the majority of dengue viral infections are self-limiting, but complications may cause high morbidity and mortality. objectives. to assess the clinical profile of the dengue infection in children less than 14 years of age and to evaluate the outcomes of dengue fever from september 2013 to august 2015 at the pediatric department of maharaja krishna chandra gajapati medical college, the largest tertiary care hospital of ...	2016	27213083
inferring the spatio-temporal patterns of dengue transmission from surveillance data in guangzhou, china.	dengue is a serious vector-borne disease, and incidence rates have significantly increased during the past few years, particularly in 2014 in guangzhou. the current situation is more complicated, due to various factors such as climate warming, urbanization, population increase, and human mobility. the purpose of this study is to detect dengue transmission patterns and identify the disease dispersion dynamics in guangzhou, china.	2016	27105350
sequence capture by hybridization to explore modern and ancient genomic diversity in model and nonmodel organisms.	the recent expansion of next-generation sequencing has significantly improved biological research. nevertheless, deep exploration of genomes or metagenomic samples remains difficult because of the sequencing depth and the associated costs required. therefore, different partitioning strategies have been developed to sequence informative subsets of studied genomes. among these strategies, hybridization capture has proven to be an innovative and efficient tool for targeting and enriching specific b ...	2016	27105841
enhancement of aedes albopictus collections by ovitrap and sticky adult trap.	in the last decades, aedes albopictus has become an increasing public health threat in tropical as well as in more recently invaded temperate areas due to its capacity to transmit several human arboviruses, among which dengue, chikungunya and zika. enhancing the efficiency of currently used collection approaches, such as ovitraps and sticky traps, is desirable for optimal monitoring of the species abundance, for assessment of the risk of arbovirus transmission and for the optimisation of control ...	2016	27102015
in vitro template-change pcr to create single crossover libraries: a case study with b. thuringiensis cry2a toxins.	during evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequence variation and function. however, no well-developed in vitro method has been established to create single-crossover libraries. here we present an in vitro template-change polymerase change reaction ...	2016	27097519
association between nutritional status and dengue infection: a systematic review and meta-analysis.	dengue infection has various clinical manifestations, often with unpredictable clinical evolutions and outcomes. several factors including nutritional status have been studied to find the relationship with dengue severity. however, the nutritional status had conflicting effects on the complication of dengue in some previous studies. therefore, we conducted a systematic review and performed a meta-analysis to analyze the association between nutritional status and the outcome of dengue infection.	2016	27097934
testing the causality between cyp9m10 and pyrethroid resistance using the talen and crispr/cas9 technologies.	recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. for studies on insecticide resistance, genome editing technologies offer some advantages over the conventional reverse genetic technique, rna interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. there were relatively abundant evidences indicating that the overexpression of a cytochrome p450 gene cyp9m10 confers s ...	2016	27095599
the wmel strain of wolbachia reduces transmission of zika virus by aedes aegypti.	zika virus (zikv) is causing an explosive outbreak of febrile disease in the americas. there are no effective antiviral therapies or licensed vaccines for this virus, and mosquito control strategies have not been adequate to contain the virus. a promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium wolbachia into aedes aegypti mosquitoes. this primarily has been proposed as a tool to control dengue virus (denv) transmission; however, e ...	2016	27364935
the threat of zika virus in sub-saharan africa - the need to remain vigilant.		2016	27303663
public health round-up.		2016	27147758
the wmel strain of wolbachia reduces transmission of chikungunya virus in aedes aegypti.	new approaches to preventing chikungunya virus (chikv) are needed because current methods are limited to controlling mosquito populations, and they have not prevented the invasion of this virus into new locales, nor have they been sufficient to control the virus upon arrival. a promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium wolbachia into aedes aegypti mosquitoes. this primarily has been proposed as a tool to control dengue viru ...	2016	27124663
unique pfk regulatory property from some mosquito vectors of disease, and from drosophila melanogaster.	arthropod-borne diseases are some of the most rapidly spreading diseases. reducing the vector population is currently the only effective way to reduce case numbers. central metabolic pathways are potential targets to control vector populations, but have not been well explored to this aim. the information available on energy metabolism, as a way to control lifespan and dispersion through flight of dipteran vectors, is inadequate.	2016	26911930
synthetic strategy and antiviral evaluation of diamide containing heterocycles targeting dengue and yellow fever virus.	high-throughput screening of a subset of the cd3 chemical library (centre for drug design and discovery; ku leuven) provided us with a lead compound 1, displaying low micromolar potency against dengue virus and yellow fever virus. within a project aimed at discovering new inhibitors of flaviviruses, substitution of its central imidazole ring led to synthesis of variably substituted pyrazine dicarboxylamides and phthalic diamides, which were evaluated in cell-based assays for cytotoxicity and ant ...	2016	27240271
resurgence of yellow fever in angola, 2015-2016.		2016	27536787
genetic drift, purifying selection and vector genotype shape dengue virus intra-host genetic diversity in mosquitoes.	due to their error-prone replication, rna viruses typically exist as a diverse population of closely related genomes, which is considered critical for their fitness and adaptive potential. intra-host demographic fluctuations that stochastically reduce the effective size of viral populations are a challenge to maintaining genetic diversity during systemic host infection. arthropod-borne viruses (arboviruses) traverse several anatomical barriers during infection of their arthropod vectors that are ...	2016	27304978
toxicity of boswellia dalzielii (burseraceae) leaf fractions against immature stages of anopheles gambiae (giles) and culex quinquefasciatus (say) (diptera: culicidae).	mosquitoes are vectors of several human pathogens, and great attention has recently been placed on insecticides from plant-derived products, in search for mosquito control agents. this study, thus, investigated the potency of boswellia dalzielii methanol leaf extract and its four fractions as mosquito ovicide, larvicide, and pupicide against anopheles gambiae and culex quinquefasciatus. the plant products were tested at the following concentrations: 125, 250, 500, 1000, and 2000 ppm on eggs and ...	2016	27279752
four emerging arboviral diseases in north america: jamestown canyon, powassan, chikungunya, and zika virus diseases.	arthropod-borne viruses, or arboviruses, are viruses that are transmitted through the bites of mosquitoes, ticks, or sandflies. there are numerous arboviruses throughout the world capable of causing human disease spanning different viral families and genera. recently, jamestown canyon, powassan, chikungunya, and zika viruses have emerged as increasingly important arboviruses that can cause human disease in north america. unfortunately, there are currently no proven disease-modifying therapies fo ...	2016	26903031
the influence of urbanization modes on the spatial circulation of flaviviruses within ouagadougou (burkina faso).	dengue is an emerging infectious disease of global significance. although this virus has been reported for a long time, its significance within the burden of diseases in west africa is not obvious, especially in burkina faso. our objective was to evaluate flavivirus presence in ouagadougou (burkina faso) and the link between anti-flavivirus antibody seroprevalence and urbanization modes. a population-based cross-sectional survey was conducted and 3015 children were enrolled from ouagadougou dist ...	2016	27973402