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a new antibacterial amino phenyl pyrrolidone derivative from a novel marine gliding bacterium rapidithrix thailandica.a recently described marine gliding bacterium rapidithrix thailandica strain tistr 1741 was isolated from biofilm specimen collected from the andaman sea in thailand. four liters fermentation broth of r. thailandica tistr 1741 cultivated in vy/2 medium were extracted with methanol to yield a novel amino phenyl pyrrolidone derivative compound (1) with antibacterial activities. the chemical structure and physico-chemical properties of 1 were investigated by spectrometry techniques. compound 1 exhi ...201424162950
sunlight mediated inactivation mechanisms of enterococcus faecalis and escherichia coli in clear water versus waste stabilization pond water.escherichia coli and enterococci have been previously reported to differ in the mechanisms and conditions that affect their sunlight-mediated inactivation in waste stabilization ponds. this study was undertaken to further characterize these mechanisms, using simulated sunlight and single strains of laboratory-grown e. coli and enterococcus faecalis, with a focus on characterizing the contribution of exogenous reactive oxygen species to the inactivation process. we found that direct damage by uvb ...201424188579
microbiological findings of vulvovaginitis in prepubertal girls.to compare vaginal culture results between prepubertal girls with and without vulvovaginitis, and obtain an overview of the most commonly encountered microbes.201424191064
co-transfer of resistance to high concentrations of copper and first-line antibiotics among enterococcus from different origins (humans, animals, the environment and foods) and clonal lineages.we studied the occurrence of diverse copper (cu) tolerance genes from gram-positive bacteria and their co-transfer with antibiotic resistance genes among enterococcus from diverse sources.201424343895
role of house flies in the ecology of enterococcus faecalis from wastewater treatment facilities.enterococci are important nosocomial pathogens, with enterococcus faecalis most commonly responsible for human infections. in this study, we used several measures to test the hypothesis that house flies, musca domestica (l.), acquire and disseminate antibiotic-resistant and potentially virulent e. faecalis from wastewater treatment facilities (wwtf) to the surrounding urban environment. house flies and sludge from four wwtf (1-4) as well as house flies from three urban sites close to wwtf-1 were ...201424337146
in vitro regulation of ccl3 and cxcl12 by bacterial by-products is dependent on site of origin of human oral fibroblasts.production of chemokines by tissue resident cells is one of the main mechanisms involved in the inflammatory infiltrate formation during inflammation. the specific ability of fibroblasts from different oral tissues such as gingiva, periodontal ligament, and dental pulp from permanent and deciduous teeth in producing the chemokines ccl3 and cxcl12 under stimulation by bacterial products commonly found in endodontic infections was investigated.201424331998
impact of enterococcal colonization and infection in solid organ transplantation recipients from the swiss transplant cohort study.the burden of enterococcal infections has increased over the last decades with vancomycin-resistant enterococci (vre) being a major health problem. solid organ transplantation is considered as a risk factor. however, little is known about the relevance of enterococci in solid organ transplantation recipients in areas with a low vre prevalence.201424330137
antibiotic resistance in enterococcus faecium clinical isolates.the worldwide ratio of enterococcus faecalis-enterococcus faecium infections is currently changing in favor of e. faecium. intrinsic and acquired antimicrobial resistance traits of this latter species can explain this evolution as well as the diffusion of hospital-adapted strains belonging to the clonal complex cc17. like other enterococci, e. faecium is naturally resistant to cephalosporins and aminoglycosides (at low level). because of its high genome plasticity, it can also acquire numerous o ...201424392717
molecular epidemiology of enterococcal bacteremia in australia.enterococci are a major cause of health care-associated infections and account for approximately 10% of all bacteremias globally. the aim of this study was to determine the proportion of enterococcal bacteremia isolates in australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterize the molecular epidemiology of the enterococcus faecalis and enterococcus faecium isolates. from 1 january to 31 december 2011, 1,079 ...201424391201
antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental listeria spp. isolates including listeria monocytogenes.the aims of this study were to assess antibiotic resistance pheno- and genotypes in foodborne, clinical, and environmental listeria isolates, as well as to elucidate the horizontal gene transfer potential of detected resistance genes. a small fraction of in total 524 listeria spp. isolates (3.1%) displayed acquired antibiotic resistance mainly to tetracycline (n = 11), but also to clindamycin (n = 4) and trimethoprim (n = 3), which was genotypically confirmed. in two cases, a tetracycline resist ...201424382842
enterococcus faecalis reconfigures its transcriptional regulatory network activation at different copper levels.a global transcriptional regulatory network was generated in the pathogenic bacterium enterococcus faecalis in order to understand how this organism can activate and coordinate its expression at different copper concentrations. the topological evaluation of the network showed common patterns described in other organisms. integrating microarray experiments allowed the identification of two sub-networks activated at low (0.05 mm cuso4) and high (0.5 mm cuso4) concentrations of copper. the analysis ...201424382465
presence and genetic environment of pleuromutilin-lincosamide-streptogramin a resistance gene lsa(e) in enterococci of human and swine origin. 201424379302
effect of nanosilver gel, chlorhexidine gluconate, and camphorated phenol on enterococcus faecalis biofilm.aim. to assess the effectiveness of nanosilver gel (nsg) in comparison to chlorhexidine gluconate (chx) and camphorated phenol (cp) against enterococcus faecalis (e.f) biofilm. methods and materials. two tests were done, methyl thiazolyl tetrazolium (mtt) assay and confocal laser scanning microscopy (clsm) analysis, to determine the effectiveness of nsg, chx, and cp on e.f biofilm. polystyrene microtiter 96- and 6-well plates were used for mtt and clsm, respectively. nanosilver gel was in three ...201427355067
study of the antibacterial efficacy of bainiku-ekisu against pathogens.the research was undertaken to determine the bacteriostatic effects of the concentrate of japanese apricot juice (bainiku-ekisu), which is a popular health food in taiwan and japan, on enterococcus faecalis atcc 29212, staphylococcus aureus atcc 25923, and escherichia coli atcc 25922. the results show that e. faecalis, s. aureus, and e. coli could be killed or inhibited by bainiku-ekisu at concentrations between 1.0 and 10.0 mg/ml. the minimum inhibitory concentration (mic) was 1 mg/ml for all s ...201426904733
seasonal variations in bacterial communities and antibiotic-resistant strains associated with green bottle flies (diptera: calliphoridae).green bottle flies occur frequently around human environments in japan. many species of green bottle flies have been studied with regard to their importance in forensic examinations or clinical therapies, but the bacterial communities associated with this group of flies have not been comprehensively investigated. in this research, 454 pyrosequencing was used to reveal the bacterial communities in green bottle flies collected in different seasons. meanwhile, the bacteria were screened with select ...201424413921
ocular penetration and efficacy of levofloxacin using different drug-delivery techniques for the prevention of endophthalmitis in rabbit eyes with posterior capsule rupture.to evaluate the effects of different drug-delivery techniques for levofloxacin (lvfx) in ocular penetration and the prevention of endophthalmitis using an aphakic rabbit model with posterior capsule rupture (pcr).201424410272
chitosan-cellulose composite for wound dressing material. part 2. antimicrobial activity, blood absorption ability, and biocompatibility.chitosan (cs), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects. in this study, the novel composites containing cs and cellulose (cel) (i.e., [cel + cs]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole ...201424407857
targeting pili in enterococcal pathogenesis.passive protection, the administration of antibodies to prevent infection, has garnered significant interest in recent years as a potential prophylactic countermeasure to decrease the prevalence of hospital-acquired infections. pili, polymerized protein structures covalently anchored to the peptidoglycan wall of many gram-positive pathogens, are ideal targets for antibody intervention, given their importance in establishing infection and their accessibility to antibody interactions. in this work ...201424452680
rapid detection of enterococcus spp. direct from blood culture bottles using enterococcus quickfish method: a multicenter investigation.the performance of a diagnostic method for detection and identification of enterococcus spp. directly from positive blood culture was evaluated in a clinical study. the method, enterococcus quickfish bc, is a second-generation peptide nucleic acid (pna) fluorescence in situ hybridization (fish) test, which uses a simplified, faster assay procedure. the test uses fluorescently labeled pna probes targeting 16s rrna to differentiate enterococcus faecalis from other enterococcus spp. by the color of ...201424439447
transcriptional response of enterococcus faecalis to sunlight.microarrays were used to investigate the transcriptional response of enterococcus faecalis to photostress. e. faecalis are gram-positive bacteria used as indicators of water quality and have been shown to vary diurnally in response to sunlight. e. faecalis in filtered seawater microcosms were exposed to artificial sunlight for 12h and then placed in the dark for 12h. transcript abundance was measured at 0, 2, 6, 12, and 24h in the sunlit microcosm and a dark control using microarrays. culturable ...201424434819
antibiofilm activity of irrigating solutions associated with cetrimide. confocal laser scanning microscopy.to evaluate the antibiofilm activity of sodium hypochlorite (naocl) and chlorhexidine (chx) solutions associated with cetrimide (ctr), and qmix using confocal laser scanning microscopy.201424433253
in vitro alkaline ph resistance of enterococcus faecalis.enterococcus faecalis is a bacterial species often found in root canals with failed endodontic treatment. alkaline pastes are widely used in endodontics because of their biocompatibility and antimicrobial activity, but this microorganism can resist alkalinity. the purpose of this study was to evaluate in vitro the alkaline ph resistance of e. faecalis for different periods up to 14 days. samples were obtained from the oral cavity of 150 patients from the endodontic clinic. the ph of the experime ...201424474287
enterococcus faecalis strain lz-11 isolated from lanzhou reach of the yellow river is able to resist and absorb cadmium.lanzhou reach of the yellow river is contaminated by cadmium (cd(ii)). the aim of this study was to screen bacterial strains that is able to resist and absorb cadmium from soil sediment and elucidate the molecular mechanism.201424471702
in vivo spread of macrolide-lincosamide-streptogramin b (mlsb) resistance--a model study in chickens.the influence of specific and non-specific antibiotic pressure on in vivo spread of macrolide-lincosamide-streptogramin b (mlsb) resistance was evaluated in this study. chickens repeatedly inoculated with enterococcus faecalis harbouring the plasmid pamβ1 carrying the erm(b) gene were perorally treated for one week with tylosin, lincomycin (both specific antibiotic pressure) and chlortetracycline (non-specific antibiotic pressure). antibiotic non-treated but e. faecalis inoculated chickens serve ...201424467930
in vitro activity of rifaximin against isolates from patients with small intestinal bacterial overgrowth.rifaximin, a non-absorbable rifamycin derivative, has published clinical efficacy in the alleviation of symptoms in patients with irritable bowel syndrome (ibs). small intestinal bacterial overgrowth (sibo) is associated with the pathogenesis of ibs. this study describes for the first time the antimicrobial effect of rifaximin against sibo micro-organisms from humans. fluid was aspirated from the third part of the duodenum from 567 consecutive patients; quantitative cultures diagnosed sibo in 11 ...201424461710
evaluation of the antibacterial efficacy of silver nanoparticles against enterococcus faecalis biofilm.the purpose of this study was to evaluate the antibacterial efficacy of silver nanoparticles (agnps) as an irrigant or medicament against enterococcus faecalis biofilms formed on root dentin.201424461420
draft genome sequence of enterococcus faecalis strain pf3, isolated from adelie penguin feces from antarctica.enterococcus faecalis is one of the leading causes of nosocomial infections and is a common commensal organism in humans and other animals. in this study, we report a draft genome sequence for the e. faecalis strain pf3, isolated from adélie penguin feces collected from warriner island, antarctica.201424459271
in vitro comparison of the antibacterial effect of three intracanal irrigants and diode laser on root canals infected with enterococcus faecalis.bacteria are the primary etiology of pulpal and periradicular pathosis. in endodontically treated teeth with persistent infections only one or a few bacterial species are present of which the most important is enterococcus faecalis. the aim of this study was to compare antibacterial efficacy of canal disinfectants including 5.25% sodium hypochlorite, 2% chlorhexidine, mtad (a mixture of doxycycline, citric acid and a detergent (tween 80) and 830 nm diode laser.201425954488
the majority of a collection of u.s. endocarditis enterococcus faecalis isolates obtained from 1974 to 2004 lack capsular genes and belong to diverse, non-hospital-associated lineages.eighty-one endocarditis-derived enterococcus faecalis isolates that were collected from individual patients in the united states between 1974 and 2004 were sequence typed and analyzed for the presence of various genes, including some previously associated with virulence. overall, using our previously described trilocus sequence typing (tlst), 44 different sequence types (sts) were found within this collection; 26 isolates were singletons (a unique tlst sequence type [st(t)]), some st(t)s contain ...201424478487
performance of vitek 2 for antimicrobial susceptibility testing of staphylococcus spp. and enterococcus spp.vitek 2 (biomérieux, inc., durham, nc) is a widely used commercial antimicrobial susceptibility testing system. we compared mic results obtained by vitek 2 to those obtained by the clinical and laboratory standards institute (clsi) broth microdilution (bmd) reference method for 134 staphylococcal and 84 enterococcal clinical isolates. nineteen agents were evaluated, including all those available on vitek 2 for testing staphylococci and enterococci. the resistance phenotypes tested included methi ...201424478467
antimicrobial susceptibility of indicator bacteria isolated from chickens in southeast asian countries (vietnam, indonesia and thailand).to determine the prevalence of indicator bacteria resistant to antimicrobials among poultry in three southeast asian countries (vietnam, indonesia and thailand), we examined the antimicrobial susceptibilities of commensal bacteria isolated from chickens. in total, 125, 117 and 180 isolates of escherichia coli, enterococcus faecalis and enterococcus faecium, respectively, were used to test for antimicrobial susceptibility. bacterial resistance to antimicrobial treatment was most frequently observ ...201424476850
prevalence of the aminoglycoside phosphotransferase genes aph(3')-iiia and aph(3')-iia in escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus aureus isolates in austria.the aminoglycoside phosphotransferase aph(3')-iia primarily inactivates kanamycin and neomycin, whilst aph(3')-iiia also inactivates amikacin. the aim of this study was to determine the frequency of both resistance genes in major human pathogens to obtain their baseline prevalence in the gene pool of these bacterial populations in austria. in total, 10 541 escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus ...201424194558
photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen.treatment of infected teeth presents two major challenges: persistence of the bacterial-biofilm within root canals after treatment and compromised structural integrity of the dentin hard-tissue. in this study bioactive polymeric chitosan nanoparticles functionalized with rose-bengal, csrbnp were developed to produce antibiofilm effects as well as stabilize structural-integrity by photocrosslinking dentin-collagen. csrbnp were less toxic to fibroblasts and had significant antibacterial activity e ...201424200522
lipoteichoic acid of streptococcus mutans interacts with toll-like receptor 2 through the lipid moiety for induction of inflammatory mediators in murine macrophages.streptococcus mutans is a pathogenic gram-positive bacterium that is closely associated with dental caries and subsequent pulpal inflammation. although lipoteichoic acid (lta) is considered a major virulence factor of gram-positive bacteria, little is known about the innate immunity to s. mutans lta. in this study, we purified lta from s. mutans (sm.lta) through n-butanol extraction, hydrophobic interaction column chromatography, and ion-exchange column chromatography to investigate its immunolo ...201424216318
biofilm mediates enterococcus faecalis adhesion, invasion and survival into bovine mammary epithelial cells.we proposed in this study that during intramammary infection, biofilm formation may facilitate adherence and colonization of enterococcus faecalis to mammary gland epithelium. this was established by comparing six different ent. faecalis isolates with different biofilm-forming profiles for their adhesive, invasive and survival capabilities to bovine mammary epithelial cell line (mac-t). our results showed increased ability of the biofilm-producer ent. faecalis strains to adhere, invade and survi ...201424224825
friends group: clinical and microbiological characteristics of post-filtering surgery endophthalmitis.to study the clinical and microbiological characteristics as well as the prognostic factors for post-filtering surgery endophthalmitis.201424248809
boosted rat natural xenoantibodies cross-react with enterococcus faecalis by targeting melibiose and l-rhamnose.natural antibodies include a subset described as xenoantibodies considered to be directed at microorganisms and also cross-react with antigens of unrelated species. in this study, we generated t-cell-independent (ti) and t-cell-dependent (td) xenoantibodies in lewis rats with hamster and pig blood injections. ti anti-hamster and anti-pig igm and igg xenoantibodies cross-reacted with enterococcus faecalis but not with escherichia coli isolated from the blood of lewis rats after cecal ligation and ...201424246417
immunostimulatory cpg motifs in the genomes of gut bacteria and their role in human health and disease.toll-like receptor (tlr) signalling plays an important role in epithelial and immune cells of the intestine. tlr9 recognizes unmethylated cpg motifs in bacterial dna, and tlr9 signalling maintains the gut epithelial homeostasis. here, we carried out a bioinformatic analysis of the frequency of cpg motifs in the genomes of gut commensal bacteria across major bacterial phyla. the frequency of potentially immunostimulatory cpg motifs (all cpg hexamers) or purine-purine-cg-pyrimidine-pyrimidine hexa ...201424255136
antimicrobial efficacy of photodynamic therapy and sodium hypochlorite on monoculture biofilms of enterococcus faecalis at different stages of development.as the enterococcus faecalis biofilm ages, it is likely that bacteria in mature and old biofilms will become more resistant to commonly used antibacterial irrigants. photodynamic therapy (pdt) has been used to kill resistant bacteria organized in a biofilm. this study aimed to investigate the antimicrobial effectiveness of 1% sodium hypochlorite (naocl), 2.5% naocl, and pdt on e. faecalis biofilm at different stages of development.201424308707
comparison of peptide nucleic acid fluorescence in situ hybridization assays with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry for the identification of bacteria and yeasts from blood cultures and cerebrospinal fluid cultures.peptide nucleic acid fluorescence in situ hybridization (pna fish) is a molecular diagnostic tool for the rapid detection of pathogens directly from liquid media. the aim of this study was to prospectively evaluate pna fish assays in comparison with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) identification, as a reference method, for both blood and cerebrospinal fluid (csf) cultures, during a 1-year investigation. on the basis of the ...201424304149
impact of actin on adhesion and translocation of enterococcus faecalis.this study focuses on the impact of actin on adhesion and translocation of enterococcus (e.) faecalis og1rf, e. faecalis symbioflor(®), and e. faecalis v583. insight into the role of actin aggregation in the mediation of bacterial adhesion and translocation was provided by a two-chamber translocation assay, which employed ptk6 cells. determination of translocation rates, cytochalasin d treatment, and laser scanning confocal microscopic observation revealed actin as a predominant brace for entero ...201424362949
residual activity of cetrimide and chlorhexidine on enterococcus faecalis-infected root canals.effective final irrigation regimen is an important step in order to achieve better disinfection and ensure residual antimicrobial effects after root canal preparation. the aim of this study was to compare the residual antimicrobial activity of 0.2% cetrimide, and 0.2% and 2% chlorhexidine in root canals infected with enterococcus faecalis. biofilms of e. faecalis were grown on uniradicular roots for 4 weeks. after root canal preparation, root canals were irrigated with 17% ethylenediaminetetraac ...201424357857
genetic basis for vancomycin-enhanced cephalosporin susceptibility in vancomycin-resistant enterococci revealed using counterselection with dominant-negative thymidylate synthase.antibiotic-resistant enterococci are major causes of hospital-acquired infections. all enterococci are intrinsically resistant to most cephalosporins, antibiotics in the beta-lactam family that impair peptidoglycan synthesis by inactivating the transpeptidases responsible for cross-linking. in addition, clinical isolates of enterococci often possess acquired resistance to vancomycin, a glycopeptide antibiotic that impairs peptidoglycan biosynthesis by a mechanism distinct from that of the beta-l ...201424366749
pathways and roles of wall teichoic acid glycosylation in staphylococcus aureus.the thick peptidoglycan layers of gram-positive bacteria are connected to polyanionic glycopolymers called wall teichoic acids (wta). pathogens such as staphylococcus aureus, listeria monocytogenes, or enterococcus faecalis produce wta with diverse, usually strain-specific structure. extensive studies on s. aureus wta mutants revealed important functions of wta in cell division, growth, morphogenesis, resistance to antimicrobials, and interaction with host or phages. while most of the s. aureus ...201424365646
nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system.enterococci are ubiquitous inhabitants of the gastrointestinal (gi) tract. however, antibiotic-resistant enterococci are also major causes of hospital-acquired infections. enterococci are intrinsically resistant to cephalosporins, enabling growth to abnormally high densities in the gi tract in patients during cephalosporin therapy, thereby promoting dissemination to other sites where they cause infection. despite its importance, many questions about the underlying basis for cephalosporin resista ...201424277024
investigation of a multiresistance gene cfr that fails to mediate resistance to phenicols and oxazolidinones in enterococcus faecalis.to investigate the basis of susceptibility to phenicols and oxazolidinones of the porcine enterococcus faecalis cppf5 despite the presence of the multiresistance gene cfr.201424272266
incidence of virulence factors in enterococci from raw and fermented meat and biofilm forming capacity at 25°c and 37°c.twenty-nine enterococcus strains from raw and fermented meat products were screened for the presence of virulence genes, including those for aggregation substances (asa1 and asa373), cytolysin activator (cyla), collagen binding protein (ace), endocarditis antigen (efaa), enterococcal surface protein (esp) and gelatinase (gele). virulence gene occurrence, expression of gelatinase and pheromone aggregation was greater in enterococcus faecalis than in enterococcus faecium strains. all e. faecalis a ...201424291183
characterization of lactic acid bacteria isolated from infant faeces as potential probiotic starter cultures for fermented sausages.a total of 109 lactic acid bacteria isolated from infant faeces were identified by partial 16s rrna, cpn60 and/or phes sequencing. lactobacillus was the most prevalent genus, representing 48% of the isolates followed by enterococcus (38%). lactobacillus gasseri (21%) and enterococcus faecalis (38%) were the main species detected. a further selection of potential probiotic starter cultures for fermented sausages focused on lactobacillus as the most technologically relevant genus in this type of p ...201424290655
decolourisation of acid orange 7 recalcitrant auto-oxidation coloured by-products using an acclimatised mixed bacterial culture.this study focuses on the biodegradation of recalcitrant, coloured compounds resulting from auto-oxidation of acid orange 7 (ao7) in a sequential facultative anaerobic-aerobic treatment system. a novel mixed bacterial culture, bac-zs, consisting of brevibacillus panacihumi strain zb1, lysinibacillus fusiformis strain zb2, and enterococcus faecalis strain zl bacteria were isolated from environmental samples. the acclimatisation of the mixed culture was carried out in an ao7 decolourised solution. ...201424293297
clonal diversity in biofilm formation by enterococcus faecalis in response to environmental stress associated with endodontic irrigants and medicaments.to determine whether clonal diversity within e. faecalis affects biofilm formation when exposed to antimicrobial compounds found in endodontic medicaments and irrigants.201524749689
comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white-coloured mineral trioxide aggregate.to comparatively examine the cell attachment, cytotoxicity, and antibacterial activity of radiopaque dicalcium silicate cement (rdsc) and proroot white-coloured mineral trioxide aggregate (wmta).201524802368
formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (apdt): studies on curcumin and curcuminoids lii.bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (apdt) are needed. curcumin is under investigation as a potential photosensitizer in apdt.201524842546
demography and burden of care associated with patients readmitted for urinary tract infection.urinary tract infection (uti) is one of the most prevalent admission diagnoses in hospital-based clinical practice. despite its frequency, few data are available regarding its demographics and economic implications.201524863498
characterisation of the antibacterial effect of polyethyleneimine nanoparticles in relation to particle distribution in resin composite.to characterise the antibacterial effect of resin composite incorporating cross-linked quaternised polyethyleneimine (qpei) nanoparticles in relation to their distribution in the bulk material.201524881908
the surface rhamnopolysaccharide epa of enterococcus faecalis is a key determinant of intestinal colonization.enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients. the pathogenesis of e. faecalis infection relies in part on its capacity to colonize the gut. following disruption of intestinal homeostasis, e. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream. to identify and characterize e. faecalis genes that are key to intestinal colonization, our strategy consisted in scr ...201525035517
autoimmune neutropenia of infancy with recurrent urinary tract infections: a case report.autoimmune neutropenia of infancy is characterized by minor intercurrent infections despite severe neutropenia; severe bacterial infections are uncommon. an infant developed recurrent urinary tract infections at 9 and 11 months of age. the identified uropathogens were escherichia coli and enterococcus faecalis, respectively. empirical treatment with carbapenems, as broad-spectrum antibiotics, promptly resolved the infection without sequelae. febrile neutropenic children with cancer and autoimmun ...201524942028
assessment of dentinal tubule invasion capacity of enterococcus faecalis under stress conditions ex vivo.to investigate the dentinal tubule invasion capacity of enterococcus faecalis under alkaline and energy starvation stress conditions.201524872016
production of lactic acid using a new homofermentative enterococcus faecalis isolate.lactic acid is an intermediate-volume specialty chemical for a wide range of food and industrial applications such as pharmaceuticals, cosmetics and chemical syntheses. although lactic acid production has been well documented, improved production parameters that lead to reduced production costs are always of interest in industrial developments. in this study, we describe the production of lactic acid at high concentration, yield and volumetric productivity utilizing a novel homofermentative, fac ...201524894833
modified lactic acid bacteria detect and inhibit multiresistant enterococci.we designed lactococcus lactis to detect enterococcus faecalis. upon detection, l. lactis produce and secrete antienterococcal peptides. the peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of l. lactis. the enterococcal sex pheromone ccf10 serves as the signal for detection. expression vectors derived from pcf10, a ccf10-responsive e. faecalis sex-pheromone conjugative plasmid, were engineered in l. lactis for the detection system. recombinant host strain ...201524896372
commensal bacteria drive endogenous transformation and tumour stem cell marker expression through a bystander effect.commensal bacteria and innate immunity play a major role in the development of colorectal cancer (crc). we propose that selected commensals polarise colon macrophages to produce endogenous mutagens that initiate chromosomal instability (cin), lead to expression of progenitor and tumour stem cell markers, and drive crc through a bystander effect.201524906974
effectiveness of ktp laser versus 980 nm diode laser to kill enterococcus faecalis in biofilms developed in experimentally infected root canals.this study aimed to evaluate the antibacterial action of ktp (potassium-titanyl-phosphate) laser irradiations (compared with 980 nm diode laser), associated with conventional endodontic procedures, on enterococcus faecalis biofilms. fifty-six dental roots with single canals were prepared with ni-ti rotary instruments, autoclaved, inoculated with an e. faecalis suspension and incubated for 72 h. they were randomly allocated to control and treatment groups. laser parameters were as follows: power ...201524588799
cytotoxicity, interaction with dentine and efficacy on multispecies biofilms of a modified salt solution intended for endodontic disinfection in a new in vitro biofilm model.to investigate the cytotoxicity of a modified salt solution (mss) and evaluate the antimicrobial properties of mss on in vitro biofilm models.201524702260
in vitro efficacy of antibiotic beads in treating abdominal vascular graft infections.abdominal aortic vascular graft infection often involves several different organisms. antibiotic polymethyl methacrylate (pmma) beads may be effective in controlling infection after débridement, but bacterial species identification and antibiotic susceptibility are often not available at the time of operation, generating a need for a broad-spectrum drug combination for empirical use. we sought to determine an effective antibiotic in pmma beads for use in abdominal vascular graft infection.201524745942
urinary tract infection caused by enterococcus isolates: aetiology and antimicrobial resistance patterns.the purpose of this study was to analyse the data on resistance in enterococcus faecalis and e. faecium isolated from urine samples of inpatients from january 2010 to december 2013. compared to e. faecalis, e. faecium isolates were significantly more resistant to ampicilin, nitrofurantoin, and ciprofloxacin in the antimicrobial agents evaluated. both species showed higher resistance to high-level gentamicin. the rate of vancomycin-resistant enterococci (vre) in e. faecium was higher as compared ...201524835052
definitive structural assessment of enterococcal diheteroglycan.enterococcus faecalis is one of most important nosocomial and often multi-antibiotic resistant pathogens responsible for infections that are difficult to treat. previously, a cell-wall polysaccharide termed diheteroglycan (dhg) was isolated and characterized as a promising vaccine candidate. however, the configuration of its lactic acid (la) residue attached to the galactofuranoside unit was not assessed, although it influences conformation of dhg chain in terms of biological recognition and imm ...201525421964
in vitro and in vivo activity of hyperimmune globulin preparations against multiresistant nosocomial pathogens.we compared different immunoglobulin preparations containing igg (intraglobin/intratect) or a mixture of igg, iga, and igm (pentaglobin) to assess the opsonic and protective efficacy of human immunoglobulin preparations against multiresistent nosocomial pathogens.201525428225
the question of pathogen quantification in disinfected graywater.graywater (gw) reuse for irrigation is recognized as a sustainable solution for water conservation. one of the major impediments to gw reuse is the presence of pathogenic microorganisms. this study monitored three similar on-site gw treatment systems bi-monthly over the course of a year to compare the presence of pathogens and indicators in raw, biologically treated, and biologically treated and disinfected [by chlorine and ultraviolet light (uv)] gw. the systems were designed to allow the testi ...201525437766
enterococcus faecalis pcf10-encoded surface proteins prga, prgb (aggregation substance) and prgc contribute to plasmid transfer, biofilm formation and virulence.enterococcus faecalis pcf10 transfers at high frequencies upon pheromone induction of the prgq transfer operon. this operon codes for three cell wall-anchored proteins - prga, prgb (aggregation substance) and prgc - and a type iv secretion system through which the plasmid is delivered to recipient cells. here, we defined the contributions of the prg surface proteins to plasmid transfer, biofilm formation and virulence using the caenorhabditis elegans infection model. we report that a combination ...201525431047
antimicrobial bioassay-guided fractionation of a methanol extract of eupatorium triplinerve.eupatorium triplinerve vahl (asteraceae), popularly known as japana, is widely used in folk medicine, due its analgesic, anticoagulant, antianorexic, antiparasitic, anthelmintic, sedative, antifungal, and antibacterial properties.201525430540
antimicrobial activity of chlorhexidine, peracetic acid and sodium hypochlorite/etidronate irrigant solutions against enterococcus faecalis biofilms.to evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (naocl) and associated with 9% hebp (naocl/hebp), 2% peracetic acid (paa) and 2% chlorhexidine (chx), on the viability of enterococcus faecalis biofilms attached to dentine.201525515403
photoactivation of curcumin and sodium hypochlorite to enhance antibiofilm efficacy in root canal dentin.to test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against enterococcus faecalis biofilms in vitro.201525462576
atomic force microscopy visualization of injuries in enterococcus faecalis surface caused by er,cr:ysgg and diode lasers.to visualize by atomic force microscopy the alterations induced on enterococcus. faecalis surface after treatment with 2 types of laser: erbium chromium:yttrium-scandium-gallium-garnet (er,cr:ysgg) laser and diode laser.201525475770
the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening.the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ...201525475270
removal of total and antibiotic resistant bacteria in advanced wastewater treatment by ozonation in combination with different filtering techniques.elimination of bacteria by ozonation in combination with charcoal or slow sand filtration for advanced sewage treatment to improve the quality of treated sewage and to reduce the potential risk for human health of receiving surface waters was investigated in pilot scale at the sewage treatment plant eriskirch, baden-wuerttemberg/germany. to determine the elimination of sewage bacteria, inflowing and leaving wastewater of different treatment processes was analysed in a culture-based approach for ...201525497174
transfer of tetracycline resistance genes with aggregation substance in food-borne enterococcus faecalis.enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (as) and inducible sex pheromones to exchange genetic elements in food matrix. to evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne e. faecalis were collected in korea. among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(m) and tet(l) genes together, followed by tet(m) (19.6 %), and tet(l) (6.8 %) alone. also, we ...201525487115
outcome of aortic valve replacement for active infective endocarditis in patients on chronic hemodialysis.the high risk of morbidity and mortality for patients on hemodialysis who are undergoing cardiac surgery is increased for those with active infective endocarditis (aie). this retrospective observational single-center study evaluated the impact of chronic hemodialysis on the outcome of aortic valve replacement in patients with aortic aie.201525483000
biosynthesis of silver nanoparticles using ethanolic petals extract of rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities.the present study was aimed at biosynthesis of silver nanoparticles (agnps) using ethanolic extract of rose (rosa indica) petals and testing their potential antibacterial activity using selective human pathogenic microbes, anticancer activity using human colon adenocarcinoma cancer cell line hct 15 as well as anti-inflammatory activity using rat peritoneal macrophages in vitro. the biologically synthesized agnps were also characterized by uv-visible spectroscopy, scanning electron microscopy (se ...201525481491
the use of the replication region of plasmid prs7 from oenococcus oeni as a putative tool to generate cloning vectors for lactic acid bacteria.a chimeric plasmid, prs7rep (6.1 kb), was constructed using the replication region of prs7, a large plasmid from oenococcus oeni, and pem64, a plasmid derived from pij2925 and containing a gene for resistance to chloramphenicol. prs7rep is a shuttle vector that replicates in escherichia coli using its pij2925 component and in lactic acid bacteria (lab) using the replication region of prs7. high levels of transformants per µg of dna were obtained by electroporation of prs7rep into pediococcus aci ...201525479060
photodynamic therapy in root canals contaminated with enterococcus faecalis using curcumin as photosensitizer.the aim of the study was to evaluate the photodynamic therapy (pdt) effect on root canals contaminated with enterococcus faecalis using a light emitting diode (led) light and a curcumin solution (cur) as photosensitizer (ps). eighty root canals from uniradicular human teeth were prepared with protaper universal rotary system and contaminated with e. faecalis for 21 days. they were divided as: gia-pdt (cur, pre-irradiation for 5 + 5 min of irradiation); gib-pdt (cur, pre-irradiation for 5 + 10 mi ...201525502831
effects of the probiotic enterococcus faecium ncimb 10415 on selected lactic acid bacteria and enterobacteria in co-culture.enterococcus faecium ncimb 10415 is used as a probiotic for piglets and has been shown to modify the porcine intestinal microbiota. however, the mode of action of this probiotic modification is still unclear. one possible explanation is the direct growth inhibiting or stimulating effect of the probiotic on other indigenous bacteria. therefore, the aim of the present study was to examine the growth interactions of the probiotic with different indigenous porcine bacteria in vitro. reference strain ...201525519527
tyramine biosynthesis is transcriptionally induced at low ph and improves the fitness of enterococcus faecalis in acidic environments.enterococcus faecalis is a commensal bacterium of the human gut that requires the ability to pass through the stomach and therefore cope with low ph. e. faecalis has also been identified as one of the major tyramine producers in fermented food products, where they also encounter acidic environments. in the present work, we have constructed a non-tyramine-producing mutant to study the role of the tyramine biosynthetic pathway, which converts tyrosine to tyramine via amino acid decarboxylation. wi ...201525529314
using a genome-scale metabolic model of enterococcus faecalis v583 to assess amino acid uptake and its impact on central metabolism.increasing antibiotic resistance in pathogenic bacteria necessitates the development of new medication strategies. interfering with the metabolic network of the pathogen can provide novel drug targets but simultaneously requires a deeper and more detailed organism-specific understanding of the metabolism, which is often surprisingly sparse. in light of this, we reconstructed a genome-scale metabolic model of the pathogen enterococcus faecalis v583. the manually curated metabolic network comprise ...201525527553
effects of epigallocatechin gallate against enterococcus faecalis biofilm and virulence.epigallocatechin-3-gallate (egcg), the most abundant polyphenol in green tea (camellia sinesis) has been shown to exert antimicrobial effects on numerous bacterial pathogens. however its efficacy against enterococcus faecalis biofilm, which is associated with persistent root canal infection is unknown. the aims of this study were to investigate the effects of egcg against e. faecalis biofilm and virulence.201525526623
antimicrobial activity of prodigiosin isolated from serratia marcescens ufpeda 398.prodigiosin is an alkaloid and natural red pigment produced by serratia marcescens. prodigiosin has antimicrobial, antimalarial and antitumor properties and induces apoptosis in t and b lymphocytes. these properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries. the aim of the present study was to evaluate the antimicrobial activity of prodigiosin against pathogenic micro-organisms. the red pigments produced by s. marcescens exhibited a ...201525549906
bactericidal efficacy of cold plasma at different depths of infected root canals in vitro.cold plasma (cp) has been shown to be effective even against multiresistant microorganisms. as previous investigations on the effect of cp in root canals showed promising results, the aim of the present study was to analyze the bactericidal efficacy of cp in different depths of infected dentin.201526962378
detection of listeria monocytogenes with short peptide fragments from class iia bacteriocins as recognition elements.we employed a direct peptide-bacteria binding assay to screen peptide fragments for high and specific binding to listeria monocytogenes. peptides were screened from a peptide array library synthesized on cellulose membrane. twenty four peptide fragments (each a 14-mer) were derived from three potent anti-listerial peptides, leucocin a, pediocin pa1, and curvacin a, that belong to class iia bacteriocins. fragment leu10 (geafsagvhrlang), derived from the c-terminal region of leucocin a, displayed ...201525548942
population biology of intestinal enterococcus isolates from hospitalized and nonhospitalized individuals in different age groups.the diversity of enterococcal populations from fecal samples from hospitalized (n = 133) and nonhospitalized individuals (n = 173) of different age groups (group i, ages 0 to 19 years; group ii, ages 20 to 59 years; group iii, ages ≥60 years) was analyzed. enterococci were recovered at similar rates from hospitalized and nonhospitalized persons (77.44% to 79.77%) of all age groups (75.0% to 82.61%). enterococcus faecalis and enterococcus faecium were predominant, although seven other enterococcu ...201525548052
the catastrophic journey of a retained temporary epicardial pacemaker wire leading to enterococcus faecalis endocarditis and subsequent stroke.a 52-year-old man underwent triple coronary artery bypass graft (cabg). three months later, he presented to the emergency room with dizziness and left-sided weakness. workup revealed right frontal parenchymal haemorrhage; also, two retained temporary epicardial pacemaker wires (tepw) from his previous cabg were observed, one of which had significantly migrated. one wire was found in the soft tissues below the heart; the other transversed the mediastinum from the expected location of the right at ...201525568268
enterococcus faecalis with capsule polysaccharides type 2 and biofilm-forming capacity in indonesians requiring endodontic treatment.the aim of the present study was to evaluate the genetic diversity of enterococcus faecalis (e. faecalis) strains isolated from saliva and infected root canal samples from indonesians requiring endodontic treatment.201525641820
different vana elements in e. faecalis and in e. faecium suggest at least two origins of tn1546 among vre in a brazilian hospital.in 2009 during surveillance in a brazilian hospital, many patients were confirmed to be colonized by vancomycin-resistant enterococci (vre) and a few infection cases occurred. among 14 isolates of enterococcus faecalis, most had the same pulsotype, virulence profile (agg(+)elra(+)gele(+)), and were sequence type (st)103, indicating dissemination of a clone. the 47 enterococcus faecium were separated into four pulsotypes, the predominant virulence profile being esp(+)acm(+). all of them harbored ...201525588068
characterisation of the efficacy of endodontic medications using a three-dimensional fluorescent tooth model: an ex vivo study.the purpose of this study was to establish a three-dimensional fluorescent tooth model to investigate bacterial viability against intra-canal medicaments across the thickness and surface of root dentine. dental microbial biofilms (enterococcus faecalis and streptococcus mutans) were established on the external root surface and bacterial kill was monitored over time against intra-canal medicament (ca(oh)2 ) using fluorescent microscopy in conjunction with baclight syto9 and propidium iodide stain ...201525583457
l-lactate production from biodiesel-derived crude glycerol by metabolically engineered enterococcus faecalis: cytotoxic evaluation of biodiesel waste and development of a glycerol-inducible gene expression system.biodiesel waste is a by-product of the biodiesel production process that contains a large amount of crude glycerol. to reuse the crude glycerol, a novel bioconversion process using enterococcus faecalis was developed through physiological studies. the e. faecalis strain w11 could use biodiesel waste as a carbon source, although cell growth was significantly inhibited by the oil component in the biodiesel waste, which decreased the cellular nadh/nad(+) ratio and then induced oxidative stress to c ...201525576618
the new schiff base 4-[(4-hydroxy-3-fluoro-5-methoxy-benzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydro-pyrazol-3-one: experimental, dft calculational studies and in vitro antimicrobial activity.the synthesized schiff base, 4-[(4-hydroxy-3-fluoro-5-methoxy-benzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydro-pyrazol-3-one (i), has been characterized by (13)c nmr, (1)h nmr, 2d nmr ((1)h-(1)h cosy and (13)c apt), ft-ir, uv-vis and x-ray single-crystal techniques. molecular geometry of the compound i in the ground state, vibrational frequencies and chemical shift values have been calculated by using the density functional method (dft) with 6-311++g(d,p) basis set. the obtained results indi ...201525574656
natural sesquiterpene lactones enhance oxacillin and gentamicin effectiveness against pathogenic bacteria without antibacterial effects on beneficial lactobacilli.this is a report on the synergistic interactions (sis) between melampolide-type sesquiterpene lactones 1-8 from acanthospermum hispidum dc., and oxacillin or gentamicin, against four pathogenic strains of staphylococcus aureus and enterococcus faecalis; two of them were multi-resistant strains obtained from chronic infectious processes. our results showed that all associations of 1-8 with antibiotics (atbs) are more effective than pure atbs to control pathogenic strains of s. aureus and e. faeca ...201525641272
antibacterial effect of butyryl alkannin from arnebia euchroma against vancomycin-resistant pathogens of enterococcus faecalis causing urinary tract infections.this study was carried out to investigate the biomedicinal potential of a bioactive marker component, butyryl alkannin, isolated from n-hexane root extract of arnebia euchroma against various vancomycin-resistant enterococcus (vre) isolates of enterococcus faecalis causing urinary tract infections. as a result, butyryl alkannin showed significant antibacterial activity against multidrug-resistant e. faecalis pathogens of vre as minimum inhibitory concentration values which were found in the rang ...201525635653
ef0176 and ef0177 from enterococcus faecalis v583 are substrate-binding lipoproteins involved in abc transporter mediated ribonucleoside uptake.one of the abc transporter systems in enterococcus faecalis v583 is encoded by the ef0176-ef0180 gene cluster, which differs from orthologous operons in related bacteria in that it contains two genes putatively encoding substrate-binding proteins (sbps). these sbps, ef0176 and ef0177, have previously been identified on the surface of e. faecalis. by phenotypic studies of single and double knockout mutants, we show here that ef0176 and ef0177 are specific for ribonucleosides and, by inference, th ...201525635276
in vitro activity of linezolid as assessed through the 2013 leader surveillance program.the 2013 leader surveillance program monitored the in vitro activity of linezolid and comparator agents against gram-positive bacteria at 60 medical centers in the united states. a total of 7183 pathogens were contributed from 6 predetermined pathogen groups. the groups were staphylococcus aureus, coagulase-negative staphylococci, enterococci, streptococcus pneumoniae, β-hemolytic streptococci, and viridans group streptococci. the mic90 value for each of the 6 pathogen groups was 1 μg/ml. suscep ...201525633420
ex vivo evaluation of three instrumentation techniques on e. faecalis biofilm within oval shaped root canals.the objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with enterococcus faecalis. forty-five human lower premolars were infected with a culture of e. faecalis (atcc 29212) for 28 days. five other teeth that were neither contaminated nor instrumented were used as controls. the 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (k- ...201525627890
update of the telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2013), when applying the revised susceptibility testing method.a revised broth microdilution susceptibility testing method for telavancin was approved by the food and drug administration (fda). telavancin activity was assessed against gram-positive pathogens collected worldwide (2013) using the revised method. a total of 12,346 isolates from 90 sites were included as part of the telavancin international surveillance program for the americas, europe, and asia-pacific. telavancin had mic50 and mic90 values of 0.03 and 0.06 μg/ml, respectively, against staphyl ...201525618421
dietary enterococcus faecalis lab31 improves growth performance, reduces diarrhea, and increases fecal lactobacillus number of weaned piglets.lactic acid bacteria (lab) have been shown to enhance performance of weaned piglets. however, few studies have reported the addition of lab enterococcus faecalis as alternatives to growth promoting antibiotics for weaned piglets. this study evaluated the effects of dietary e. faecalis lab31 on the growth performance, diarrhea incidence, blood parameters, fecal bacterial and lactobacillus communities in weaned piglets. a total of 360 piglets weaned at 26 ± 2 days of age were randomly allotted to ...201525617897
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