Publications

destabilization of coxsackievirus b3 genome integrated with enhanced green fluorescent protein gene.	aims: to evaluate the stability of coxsackievirus b (cvb) genome integrated with the enhanced green fluorescent protein gene (egfp) and provide valuable information for the use of the recombinant cvb variant. methods: a cvb3 variant expressing egfp was constructed by insertion of the egfp open-reading frame (orf) at the 5' end of cvb3 orf. the recombinant virus cvb3-egfp was serially passaged in hela cells. the deletions in the cvb3-egfp genome around egfp were examined by reverse transcription ...	2011	21242657
[construction of the recombinant adenovirus expressing the cvb3 svp1-c3d3 protein and study on its immunological effects in mice].	to construct recombinant adenovirus ad/c3d3-svp1 and investigate the immune effects against coxsackievirus infection in mouse.	2011	21208565
a 7-month-old boy with breathing problems.		2011	21210593
evaluation of human enteric viruses in surface water and drinking water resources in southern ghana.	an estimated 884 million people worldwide do not have access to an improved drinking water source, and the microbial quality of these sources is often unknown. in this study, a combined tangential flow, hollow fiber ultrafiltration (uf), and real-time pcr method was applied to large volume (100 l) groundwater (n = 4), surface water (n = 9), and finished (i.e., receiving treatment) drinking water (n = 6) samples for the evaluation of human enteric viruses and bacterial indicators. human enteric v ...	2011	21212196
inactivation of murine norovirus, feline calicivirus and echovirus 12 as surrogates for human norovirus (nov) and coliphage (f+) ms2 by ultraviolet light (254 nm) and the effect of cell association on uv inactivation.	to determine inactivation profiles of three human norovirus (nov) surrogate viruses and coliphage ms2 by ultraviolet (uv) irradiation and the protective effect of cell association on uv inactivation.	2011	21214604
[molecular diagnosis of respiratory enterovirus infections: use of pcr and molecular identification for a best approach of the main circulating strains during 2008].	the pcr assays are currently used in diagnosis of enterovirus (ev) meningitis. nevertheless, the use of molecular diagnosis of ev should be investigated in respiratory tract infections (rti).	2011	20828940
prevalence of human coronaviruses in adults with acute respiratory tract infections in beijing, china.	human coronaviruses (hcovs) are a common etiological agent of acute respiratory tract infections. hcov infections, especially those caused by the two hcovs identified most recently, nl63 and hku-1, have not been characterized fully. to evaluate the prevalence and clinical presentations of hku1 and nl63 in adults with acute respiratory tract infections, an investigation of hcov infections in beijing, china from 2005 to 2009 was performed by using reverse transcriptase pcr assays and sequencing an ...	2011	21181925
neutralization of different echovirus serotypes by individual lots of intravenous immunoglobulin.	replacement therapy using intravenous immunoglobulin (ivig) preparations in people with antibody deficiencies is effective in preventing the majority of common bacterial and viral infections, yet echovirus break-through infections have occurred. currently, only limited information on neutralization capacity variability of individual ivig lots against the different echovirus serotypes is available. infectivity assays were established for the most prevalent echovirus serotypes (e 9, e 11, e 13, an ...	2011	21181927
detection of enteroviruses from clinical specimens.	enteroviruses are positive stranded rna viruses belonging to the genus enterovirus of the picornaviridae family. human enteroviruses are transmitted through the fecal-oral route and have been shown to cause mild to life-threatening diseases. various diagnostic methods have been developed to detect enteroviruses from clinical specimens but many were impeded by requirements for special reagents, lengthy procedures, low sensitivity or cross-reactivity. this chapter describes rapid and highly sensit ...	2011	21116796
simultaneous detection and differentiation of respiratory syncytial virus and other respiratory viral pathogens.	rapid and accurate detection of respiratory syncytial virus (rsv) provides pathogen-specific diagnosis, allows implementation of appropriate infection control measures, and improves patient management. one diagnostic challenge is that respiratory infections, which can be caused by several viral pathogens including rsv, usually present with similar signs and symptoms that are nearly indistinguishable by clinical diagnosis. we have described in the chapter a rapid, high-throughput laboratory techn ...	2011	21116808
the role of viral infections in the development of dilated cardiomyopathy.	enteroviruses (evs) are the most frequent pathogens in myocarditis and in the subsequently developing dilated cardiomyopathy as well. furthermore, persistence of other viruses might play a pathogenic role in the evolution from myocarditis to dilated cardiomyopathy. explanted heart of 28 patients, who underwent heart transplantation were screened for ev, adv3 and hhv6 sequences in order to assess the incidence of cardiac viral infection that may be implicated in the pathogenesis of cardiomyopathy ...	2011	20853075
selenium in human health and disease.	this review covers current knowledge of selenium in the environment, dietary intakes, metabolism and status, functions in the body, thyroid hormone metabolism, antioxidant defense systems and oxidative metabolism, and the immune system. selenium toxicity and links between deficiency and keshan disease and kashin-beck disease are described. the relationships between selenium intake/status and various health outcomes, in particular gastrointestinal and prostate cancer, cardiovascular disease, diab ...	2011	20812787
is selenium deficiency really the cause of keshan disease?	keshan disease (kd) is a dilated cardiomyopathy closely related with a diet deficient in the mineral selenium. it is named for the northeastern chinese county keshan, where the disease prevalence is high because of selenium-deficient soil. kd is a gene-environment interaction disease. here, we used stepwise multiple regression analysis to analyze the risk factors of the disease and the main clinical features in 71 kd patients and 290 controls. the variables analyzed included age, sex, family his ...	2011	20574681
repeated genomic transfers from echovirus 30 to echovirus 6 lineages indicate co-divergence between co-circulating populations of the two human enterovirus serotypes.	human echovirus types 6 (e-6) and 30 (e-30) cause seasonal epidemics of aseptic meningitis. these two enteroviruses are frequently observed in co-circulation, an epidemiological pattern that is prerequisite for the occurrence of dual infections, which can lead to recombination between co-infecting virus strains. viral sequences were determined at loci 1d (vp1 capsid protein) and 3cd (non structural proteins) in 49 e-6 strains recovered in a single geographical region in france from 1999 to 2007, ...	2011	20615482
an antiviral small-interfering rna simultaneously effective against the most prevalent enteroviruses causing acute hemorrhagic conjunctivitis.	acute hemorrhagic conjunctivitis (ahc), a highly contagious eye disease, is caused primarily by either enterovirus 70 (ev70) or coxsackievirus a24 (cva24) infection. yet methods to prevent or cure ahc are not available. recent evidence has shown that small-interfering rnas (sirnas), mediators of posttranscriptional gene knockdown, can act as effective antiviral agents. thus, the authors attempted to develop a novel sirna-based anti-ahc agent effective against both ev70 and cva24.	2011	20739472
viruses and bacteria in karst and fractured rock aquifers in east tennessee, usa.	a survey of enteric viruses and indicator bacteria was carried out in eight community water supply sources (four wells and four springs) in east tennessee. seven sites derived their water from carbonate aquifers and one from fractured sandstone. four of the sites were deemed "low-risk" based on prior monitoring of fecal indicators and factors such as presence of thick layers of overlying sediments. the remaining sites were deemed "high-risk." enteric viruses (enterovirus and reovirus) were detec ...	2011	20331750
different cytokine profile and eosinophil activation are involved in rhinovirus- and rs virus-induced acute exacerbation of childhood wheezing.	because little information is available on eosinophil activation and cytokine response in virus-induced wheezing, we attempted to detect respiratory viruses and measure eosinophil cationic protein (ecp), and 27 types of cytokines/chemokines in both serum and nasal secretions from children with wheezing. this study was an observational, case-control investigation of 267 subjects, who were visited and/or hospitalized with acute respiratory symptoms (with wheezing: men, 115; women, 59; mean/median ...	2011	20408969
enteroviruses in the pathogenesis of type 1 diabetes.	the question if enteroviruses could cause beta-cell damage and type 1 diabetes has become more and more relevant when recent studies have provided new evidence supporting this scenario. one important observation is the recent discovery of ifih1 as a risk gene for type 1 diabetes. this gene is an innate immune system receptor for enteroviruses offering one possible mechanism for the diabetogenic effect of enteroviruses. this is further emphasized by the observations suggesting that the innate imm ...	2011	20424841
immunopathology of the human pancreas in type-i diabetes.	type 1 diabetes is a chronic autoimmune disease characterised by the selective destruction of pancreatic beta (β) cells. the understanding of the aetiology of this disease has increased dramatically in recent years by the study of tissue recovered from patients, from analysis of the responses of isolated islet and β-cells in tissue culture and via the use of animal models. however, knowledge of the immunopathology of type 1 diabetes in humans is still relatively deficient due largely to the diff ...	2011	20424842
onychomadesis outbreak in valencia, spain associated with hand, foot, and mouth disease caused by enteroviruses.	this report evaluates the june 2008 onychomadesis outbreak in valencia, spain. the study sample consisted of 221 onychomadesis cases and 77 nonaffected individuals who lived close to those affected. we collected data on dietary variables, hygiene products, and individual pathological histories. feces and blood specimens were collected from 44 cases and 24 controls to evaluate exposure to infectious agents. pathological background data revealed a high frequency (61%) of hand, foot, and mouth dise ...	2011	20553401
detection of human enterovirus 71 reverse transcription loop-mediated isothermal amplification (rt-lamp).	aims: in this study, a one-step, single-tube reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed and validated for the detection of human enterovirus 71 (ev71), the major aetiological agent of hand, foot and mouth disease (hfmd). methods and results: reverse transcription loop-mediated isothermal amplification assay was optimized to amplify vp1 gene in the presence of a specific primer set and bst dna polymerase at an isothermal temperature of 63°c for 1 ...	2012	22256968
MEK1-ERKs signal cascade is required for the replication of Enterovirus 71 (EV71).	The role of the MEK1-ERK signaling cascade in the replication cycle of Enterovirus 71 (EV71), the primary cause of hand, foot and mouth disease (HFMD), has been analyzed. In vitro infection with EV71 induced a biphasic activation of ERK. The two phases of activation appeared to be triggered by different mechanisms, with the first phase being activated by the binding of viral particles to the membrane receptor of host cells and the second probably being in response to the production of new virus ...	2012	22101247
Inhibition or deficiency of cathepsin B leads defects in HIV-1 Gag pseudoparticle release in macrophages and HEK293T cells.	Human immunodeficiency virus type 1 (HIV-1) egresses from infected cells through utilizing the host membrane budding mechanisms. Assembly of HIV-1 Gag particles occurs on membranes where the Gag multimers subsequently bud off and form enveloped viral particles. In certain cell types such as macrophages, HIV-1 Gag particles have shown to be released into intracellular virus containing compartments (VCC) such as late endosomes, multivesicular bodies (MVBs) or invaginated plasma membrane pockets. H ...	2012	22138708
Mutations in VP2 and VP1 capsid proteins increase infectivity and mouse lethality of enterovirus 71 by virus binding and RNA accumulation enhancement.	Enterovirus 71 (EV71) is a major cause of hand-foot-and-mouth disease. EV71 infection occasionally associates with severe neurological sequelae such as brainstem encephalitis or poliovirus-like paralysis. We demonstrated that mouse-adapted strain increases infectivity, resulting in higher cytotoxicity of neuron cells and mortality to neonatal mice than a non-adapted strain. Results pointed to EV71 capsid region determining viral infectivity and mouse lethality. Mutant virus with lysine to methio ...	2012	22078110
isolation of influenza viruses in mdck 33016pf cells and clearance of contaminating respiratory viruses.	this paper summarizes results obtained by multiplex pcr screening of human clinical samples for respiratory viruses and corresponding data obtained after passaging of virus-positive samples in mdck 33016pf cells. using the resplexii v2.0 (qiagen) multiplex pcr, 393 positive results were obtained in 468 clinical samples collected during an influenza season in germany. the overall distribution of positive results was influenza a 42.0%, influenza b 38.7%, adenovirus 1.5%, bocavirus 0.5%, coronaviru ...	2012	22119922
enteric viral pathogens in children with inflammatory bowel disease.	the causes of exacerbations of inflammatory bowel disease (ibd) are unknown. the presence of rna of an enterovirus, norovirus gi, norovirus gii, rotavirus, astrovirus, and sapovirus was sought in stool samples of 50 children (median age 12.9 years) undergoing gastrointestinal endoscopies for ibd or its exclusion (crohn's disease n = 18, ulcerative colitis n = 13, indeterminate colitis n = 2, non-ibd n = 17). viral rna was found in three fecal samples (norovirus gii n = 2, sapovirus n = 1), all i ...	2012	22170557
evaluation of human enterovirus 71 and coxsackievirus a16 specific immunoglobulin m antibodies for diagnosis of hand-foot-and-mouth disease.	abstract: background: hand-foot-and-mouth disease (hfmd) is caused mainly by the human enterovirus type 71 (hev71) and the coxsackievirus a group type 16 (cva16). large outbreaks of disease have occurred frequently in the asia-pacific region. reliable methods are needed for diagnosis of hfmd in childen. igm-capture elisa, with its notable advantages of convenience and low cost, provides a potentially frontline assay. we aimed to evaluate the newly developed igm-capture elisas for hev71 and cva1 ...	2012	22234169
enterovirus 71 disrupts interferon signaling by reducing the interferon receptor i.	the recent outbreak of enterovirus 71 (ev71) infected millions of children and caused over 1,000 deaths. to date, neither effective vaccine nor antiviral treatment is available for ev71 infection. interferons (ifns) have been successfully applied for treating patients such as hepatitis b and c viral infections for decades but failed to treat ev71 infections. here, we provide the evidence that ev71 antagonizes type i ifn signaling by reducing the level of interferon receptor 1 (ifnar1). we show t ...	2012	22258259
in vitro and in vivo evaluation of ribavirin and pleconaril antiviral activity against enterovirus 71 infection.	enterovirus 71(ev71) causes recurring outbreaks of hand, foot and mouth disease and encephalitis leading to complications or death in young children. more effective antiviral drugs are needed to prevent or reduce ev71-related disease and complications. however, there are no standard models currently in use to evaluate activity against ev71 infection both in vitro and in vivo. in this study, the activity of ribavirin and pleconaril against ev71 infection was evaluated in two models. an in vitro e ...	2012	22245989
presence and fate of coliphages and enteric viruses in three wastewater treatment plants effluents and activated sludge from tunisia.	purpose: the role of water in the transmission of infectious diseases is well defined; it may act as a reservoir of different types of pathogens. enteric viruses can survive and persist for a long time in water, maintaining infectivity in many instances. this suggests the need to include virus detection in the evaluation of the microbiological quality of waters. methods: in this study, enteric viruses (enteroviru ...	2012	22261843
detection of human enterovirus 71 and coxsackievirus a16 in children with hand, foot and mouth disease in china.	the aims of the present study were to investigate the genetic characteristics of enterovirus 71 (ev71) and coxsackievirus a16 (cva16) strains in china and to evaluate the relationship between the genotypes of cva16 and ev71 and their geographical distribution. a total of 399 stool specimens were collected from children with symptoms of hand, foot and mouth disease (hfmd) in zhejiang province. the presence of enteroviruses was determined ...	2012	22218731
when are amniotic fluid viral pcr studies indicated in prenatal diagnosis?	objective: to determine which prenatal ultrasound findings indicate the need to also obtain pcr studies for viral genome in women undergoing midtrimester amniocentesis. methods: this was a retrospective observational study on women that underwent amniotic fluid karyotyping and viral pcr testing for history or ultrasound based indication. amniotic fluid was tested for adenovirus, cytomegalovirus, respiratory syncytial virus, enterovirus, epstein-barr virus, and parvovirus b19 using multiplex pcr ...	2012	22275111
distribution of enteroviruses in hospitalized children with hand, foot and mouth disease and relationship between pathogens and nervous system complications.	abstract: background: to explore the relationship between enteroviruses and hospitalized children with hand, foot and mouth disease (hfmd) complicated with nervous system disease. 234 hospitalized hfmd patients treated in shengjing hospital, liaoning province were analyzed retrospectively. based on the presence and severity of nervous system disease, the patients were grouped as follows: general patients, severely ill patients, critically ill patients and fatal patients. based on the detected p ...	2012	22230340
meningitis and encephalitis in infants and children.	despite the availability of modern therapies, meningitis and encephalitis remain potentially life-threatening infections in children with mortality rates reaching up to 25%. treated patients are at a high risk of long term sequelae including epilepsy, learning, and behavioral disorders. the golden rule of early diagnosis and treatment to achieve a good outcome has not yet been challenged by the new, often expensive antibiotics or contemporary critical care. in this article, an updated overview o ...	2012	22273641
high detection frequency and viral loads of human rhinovirus species a to c in fecal samples; diagnostic and clinical implications.	human rhinoviruses (hrvs) can be divided into three species; hrv-a to hrv-c. up to 148 different hrv (sero)types have been identified to date. because of sequence similarity between 5'-ncr of hrvs and enteroviruses (evs), it is problematic to design ev-specific rt-pcr assays. the aims of this study were to assess the rate of false-detection of different rhinoviruses by ev rt-pcr, and to evaluate the diagnostic and clinical significance of such cross-reactivity. in vitro rna transcripts of hrv a- ...	2012	22246843
Isolation of Coxsackievirus A24 variant from patients with hemorrhagic conjunctivitis in Cuba, 2008-2009.	An outbreak of acute hemorrhagic conjunctivitis occurred in Cuba in 2008 and 2009.	2012	22074932
Experimental human rhinovirus and enterovirus interspecies recombination.	Human rhinoviruses (HRVs) and enteroviruses (HEVs), two important human pathogens, are non-enveloped, positive-sense RNA viruses of the genus Enterovirus within the family Picornaviridae. Intraspecies recombination is known as a driving force for enterovirus and, to a lesser extent, rhinovirus evolution. Interspecies recombination is much less frequent among circulating strains, and supporting evidence for such recombination is limited to ancestral events, as shown by recent phylogenetic analyse ...	2012	21940413
Modification of the untranslated regions of human enterovirus 71 impairs growth in a cell-specific manner.	Human enterovirus 71 (HEV71) is the causative agent of hand, foot, and mouth disease and associated acute neurological disease. At present, little is known about the genetic determinants of HEV71 neurovirulence. Studies of related enteroviruses have indicated that the untranslated regions (UTRs), which control virus-directed translation and replication, also exert significant influence on neurovirulence. We used an infectious cDNA clone of a subgenogroup B3 strain to construct and characterize c ...	2012	22031931
sabin and wild polioviruses from apparently healthy primary school children in northeastern nigeria.	despite significant success of the global polio eradication initiative (gpei) in nigeria, afghanistan, india, pakistan, wild poliovirus still occurs due to persistently high proportions of under and unimmunized children. the study aimed at determining the type of poliovirus often excreted into the environment. four hundred nine fecal samples collected from apparently healthy school children aged 5-16 years in borno and adamawa states, northeastern nigeria, were tested for poliovirus by tissue cu ...	2012	22170559
role of enterovirus infections in ige sensitization.	among other infectious agents, enteroviruses have been associated with protection against allergic diseases. the aim of the present study was to confirm these findings using a highly sensitive and specific neutralization antibody assay and to investigate whether the protective effect is related to certain enterovirus serotypes. antibodies against 12 enterovirus serotypes were measured in 60 children who were positive for allergen-specific ige and in 190 control children. echoviruses seemed to be ...	2012	22170547
Duck Hepatitis A Virus Possesses a Distinct Type IV Internal Ribosome Entry Site Element of Picornavirus.	Sequence analysis of duck hepatitis virus type 1 (DHV-1) led to its classification as the only member of a new genus, Avihepatovirus, of the family Picornaviridae, and so was renamed duck hepatitis A virus (DHAV). The 5' untranslated region (5' UTR) plays an important role in translation initiation and RNA synthesis of the picornavirus. Here, we provide evidence that the 651-nucleotide (nt)-long 5' UTR of DHAV genome contains an internal ribosome entry site (IRES) element that functions efficien ...	2012	22090106
detection of enterovirus rna in cerebrospinal fluid: comparison of two molecular assays.	enterovirus (ev) and human parechovirus (hpev) are a major cause of infection in childhood. a rapid diagnostic test may improve the management of patients with ev and hpev infection. the aim of this study is to evaluate the performance of the genexpert enterovirus assay (gxea) for detection of ev rna compared to a user-developed reverse-transcriptase (rt) quantitative real-time pcr (qpcr) in routine clinical practice. also a rt-qpcr assay for detection of hpev rna in different clinical samples w ...	2012	22024398
Combating enterovirus replication: State-of-the-art on antiviral research.	Enteroviruses form an important genus within the large family of Picornaviridae. They are small, non-enveloped (+)RNA viruses, many of which are important pathogens in human and veterinary science. Despite their huge medical and socio-economical impact, there is still no approved antiviral therapy at hand for the treatment of these infections. Three capsid-targeting molecules (pleconaril, BTA-798 and V-073) are in clinical development. Pleconaril and BTA-798 are in phase II clinical trials for t ...	2012	21889497
seasonal variations of 15 respiratory agents illustrated by the application of a multiplex polymerase chain reaction assay.	abstract background: nucleic acid amplification tests are increasingly being used to diagnose viral and bacterial respiratory tract infections. the high sensitivity of these tests affects our understanding of the epidemiology of respiratory tract infections. we have assessed the detection rate of a multiplex real-time polymerase chain reaction (pcr) test, with emphasis on epidemiology and seasonal distribution of the most common respiratory tract infections. methods: seven thousand eight hundr ...	2012	21867470
Detection of herpes simplex virus (1 and 2), varicella-zoster virus, cytomegalovirus, human herpesvirus 6 and enterovirus in immunocompetent tunisian patients with acute neuromeningeal disorder.	Enteroviruses (EVs) and human herpesviruses (HHVs) are involved frequently in acute neurological disorders of viral etiology. This study aimed to investigate the incidence of herpes simplex virus types-1 (HSV-1) and 2 (HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and human enteroviruses (EVs) in cerebrospinal fluid (CSF) samples of Tunisian immunocompetent patients with neuromeningeal disorders. The patients had been hospitalized at the Fattouma Bourgu ...	2012	22170549