Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| particularities of allergy in the tropics. | allergic diseases are distributed worldwide and their risk factors and triggers vary according to geographical and socioeconomic conditions. allergies are frequent in the tropics but aspects of their prevalence, natural history, risk factors, sensitizers and triggers are not well defined and some are expected to be different from those in temperate zone countries. the aim of this review is to investigate if allergic diseases in the tropics have particularities that deserve special attention for ... | 2016 | 27386040 |
| antinociceptive effect of tephrosia sinapou extract in the acetic acid, phenyl-p-benzoquinone, formalin, and complete freund's adjuvant models of overt pain-like behavior in mice. | tephrosia toxicaria, which is currently known as tephrosia sinapou (buc'hoz) a. chev. (fabaceae), is a source of compounds such as flavonoids. t. sinapou has been used in amazonian countries traditional medicine to alleviate pain and inflammation. the purpose of this study was to evaluate the analgesic effects of t. sinapou ethyl acetate extract in overt pain-like behavior models in mice by using writhing response and flinching/licking tests. we demonstrated in this study that t. sinapou extract ... | 2016 | 27293981 |
| a deep insight into the sialome of male and female aedes aegypti mosquitoes. | only adult female mosquitoes feed on blood, while both genders take sugar meals. accordingly, several compounds associated with blood feeding (i.e. vasodilators, anti-clotting, anti-platelets) are found only in female glands, while enzymes associated with sugar feeding or antimicrobials (such as lysozyme) are found in the glands of both sexes. we performed de novo assembly of reads from adult aedes aegypti female and male salivary gland libraries (285 and 90 million reads, respectively). by mapp ... | 2016 | 26999592 |
| epidemiology of chikungunya virus in bahia, brazil, 2014-2015. | chikungunya is an emerging arbovirus that is characterized into four lineages. one of these, the asian genotype, has spread rapidly in the americas after its introduction in the saint martin island in october 2013. unexpectedly, a new lineage, the east-central-south african genotype, was introduced from angola in the end of may 2014 in feira de santana (fsa), the second largest city in bahia state, brazil, where over 5,500 cases have now been reported. number weekly cases of clinically confirmed ... | 2016 | 27330849 |
| accuracy of clinical criteria and an immunochromatographic strip test for dengue diagnosis in a denv-4 epidemic. | early diagnosis of dengue infection is important for decision-making and timely implementation of therapeutic measures. although rapid ns1 assays have been used for dengue diagnosis since 2008, their performance in denv-4 cases has not yet been fully assessed. | 2016 | 26822788 |
| in silico analysis of an envelope domain iii-based multivalent fusion protein as a potential dengue vaccine candidate. | dengue virus infection is now a global problem. currently, there is no licensed vaccine or proven antiviral treatment against this virus. all four serotypes (1-4) of dengue virus can infect human. an effective dengue vaccine should be tetravalent to induce protective immune responses against all four serotypes. most of dengue vaccine candidates are monovalent, or in the form of physically mixed multivalent formulations. recently envelope protein domain iii of virus is considered as a vaccine can ... | 2016 | 26866023 |
| the risk of dengue virus transmission in dar es salaam, tanzania during an epidemic period of 2014. | in 2010, 2012, 2013 and 2014 dengue outbreaks have been reported in dar es salaam, tanzania. however, there is no comprehensive data on the risk of transmission of dengue in the country. the objective of this study was to assess the risk of transmission of dengue in dar es salaam during the 2014 epidemic. | 2016 | 26812489 |
| identification of proteins bound to dengue viral rna in vivo reveals new host proteins important for virus replication. | dengue virus is the most prevalent cause of arthropod-borne infection worldwide. due to the limited coding capacity of the viral genome and the complexity of the viral life cycle, host cell proteins play essential roles throughout the course of viral infection. host rna-binding proteins mediate various aspects of virus replication through their physical interactions with viral rna. here we describe a technique designed to identify such interactions in the context of infected cells using uv cross ... | 2016 | 26733069 |
| relevance of non-communicable comorbidities for the development of the severe forms of dengue: a systematic literature review. | patients with dengue fever and comorbidities seem to be at higher risk of developing complications and/or severe dengue compared to healthier individuals. this study systematically reviews the evidence related to comorbidities and dengue. a systematic literature review was performed in five databases (embase, pubmed, global health, scielo, cochrane) and grey literature for full-text articles since its inceptions until october 10, 2015. a total of 230 articles were retrieved. sixteen studies were ... | 2016 | 26727113 |
| antibody-dependent enhancement of dengue virus infection inhibits rlr-mediated type-i ifn-independent signalling through upregulation of cellular autophagy. | antibody dependent enhancement (ade) of dengue virus (denv) infection is identified as the main risk factor of severe dengue diseases. through opsonization by subneutralizing or non-neutralizing antibodies, denv infection suppresses innate cell immunity to facilitate viral replication. however, it is largely unknown whether suppression of type-i ifn is necessary for a successful ade infection. here, we report that both denv and denv-ade infection induce an early isg (nos2) expression through rlr ... | 2016 | 26923481 |
| in silico screening, alanine mutation, and dft approaches for identification of ns2b/ns3 protease inhibitors. | to identify the ligand that binds to a target protein with high affinity is a nontrivial task in computer-assisted approaches. antiviral drugs have been identified for ns2b/ns3 protease enzyme on the mechanism to cleave the viral protein using the computational tools. the consequence of the molecular docking, free energy calculations, and simulation protocols explores the better ligand. it provides in-depth structural insights with the catalytic triad of his51, asp75, ser135, and gly133. the md ... | 2016 | 27057355 |
| no evidence for local adaptation of dengue viruses to mosquito vector populations in thailand. | despite their epidemiological importance, the evolutionary forces that shape the spatial structure of dengue virus genetic diversity are not fully understood. fine-scale genetic structure of mosquito vector populations and evidence for genotype × genotype interactions between dengue viruses and their mosquito vectors are consistent with the hypothesis that the geographical distribution of dengue virus genetic diversity may reflect viral adaptation to local mosquito populations. to test this hypo ... | 2016 | 27099625 |
| phylogenetic analysis of dengue virus 1 isolated from south minas gerais, brazil. | dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. primary infection with a single dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. however, a subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease, called dengue hemorrhagic fever. the four dengue virus serotypes (1-4) are antigenically and genetically distinct and each ... | 2016 | 26887252 |
| the risk of dengue for non-immune foreign visitors to the 2016 summer olympic games in rio de janeiro, brazil. | rio de janeiro in brazil will host the summer olympic games in 2016. about 400,000 non-immune foreign tourists are expected to attend the games. as brazil is the country with the highest number of dengue cases worldwide, concern about the risk of dengue for travelers is justified. | 2016 | 27129407 |
| analysis of significant factors for dengue fever incidence prediction. | many popular dengue forecasting techniques have been used by several researchers to extrapolate dengue incidence rates, including the k-h model, support vector machines (svm), and artificial neural networks (ann). the time series analysis methodology, particularly arima and sarima, has been increasingly applied to the field of epidemiological research for dengue fever, dengue hemorrhagic fever, and other infectious diseases. the main drawback of these methods is that they do not consider other v ... | 2016 | 27083696 |
| first chikungunya outbreak in suriname; clinical and epidemiological features. | in june 2014, suriname faced the first chikungunya outbreak. since international reports mostly focus on hospitalized patients, the least affected group, a study was conducted to describe clinical characteristics of mainly outpatients including children. in addition, the cumulative incidence of this first epidemic was investigated. | 2016 | 27082985 |
| assessing potential countermeasures against the dengue epidemic in non-tropical urban cities. | dengue is a common mosquito-borne viral disease epidemic especially in tropical and sub-tropical regions where water sanitation is not substantially controlled. however, dengue epidemics sometimes occur in non-tropical urban cities with substantial water sanitary control. using a mathematical model, we investigate what conditions can be important for a dengue epidemic to occur in an urban city such as tokyo, where vectors are active only in summer and there are little number of vectors around ho ... | 2016 | 27072122 |
| epidemiological dynamics of an urban dengue 4 outbreak in são paulo, brazil. | dengue studies at the urban scale are scarce and required for guiding control efforts. in brazil, the burden of dengue is high and challenges city public health administrations with limited resources. here we studied the dynamics of a dengue epidemic in a single city. | 2016 | 27069820 |
| presence of three dengue serotypes in ouagadougou (burkina faso): research and public health implications. | the significant malaria burden in africa has often eclipsed other febrile illnesses. burkina faso's first dengue epidemic occurred in 1925 and the most recent in 2013. yet there is still very little known about dengue prevalence, its vector proliferation, and its poverty and equity impacts. | 2016 | 27044528 |
| fitting the incidence data from the city of campinas, brazil, based on dengue transmission modellings considering time-dependent entomological parameters. | four time-dependent dengue transmission models are considered in order to fit the incidence data from the city of campinas, brazil, recorded from october 1st 1995 to september 30th 2012. the entomological parameters are allowed to depend on temperature and precipitation, while the carrying capacity and the hatching of eggs depend only on precipitation. the whole period of incidence of dengue is split into four periods, due to the fact that the model is formulated considering the circulation of o ... | 2016 | 27010654 |
| evolutionary analysis of dengue serotype 2 viruses using phylogenetic and bayesian methods from new delhi, india. | dengue fever is the most important arboviral disease in the tropical and sub-tropical countries of the world. delhi, the metropolitan capital state of india, has reported many dengue outbreaks, with the last outbreak occurring in 2013. we have recently reported predominance of dengue virus serotype 2 during 2011-2014 in delhi. in the present study, we report molecular characterization and evolutionary analysis of dengue serotype 2 viruses which were detected in 2011-2014 in delhi. envelope genes ... | 2016 | 26977703 |
| dengue in java, indonesia: relevance of mosquito indices as risk predictors. | no vaccine is currently available for dengue virus (denv), therefore control programmes usually focus on managing mosquito vector populations. entomological surveys provide the most common means of characterising vector populations and predicting the risk of local dengue virus transmission. despite indonesia being a country strongly affected by denv, only limited information is available on the local factors affecting denv transmission and the suitability of available survey methods for assessin ... | 2016 | 26967524 |
| co-distribution and co-infection of chikungunya and dengue viruses. | chikungunya and dengue infections are spatio-temporally related. the current review aims to determine the geographic limits of chikungunya, dengue and the principal mosquito vectors for both viruses and to synthesise current epidemiological understanding of their co-distribution. | 2016 | 26936191 |
| high-throughput cis-regulatory element discovery in the vector mosquito aedes aegypti. | despite substantial progress in mosquito genomic and genetic research, few cis-regulatory elements (cres), dna sequences that control gene expression, have been identified in mosquitoes or other non-model insects. formaldehyde-assisted isolation of regulatory elements paired with dna sequencing, faire-seq, is emerging as a powerful new high-throughput tool for global cre discovery. faire results in the preferential recovery of open chromatin dna fragments that are not bound by nucleosomes, an ev ... | 2016 | 27161480 |
| projected impact of dengue vaccination in yucatán, mexico. | dengue vaccines will soon provide a new tool for reducing dengue disease, but the effectiveness of widespread vaccination campaigns has not yet been determined. we developed an agent-based dengue model representing movement of and transmission dynamics among people and mosquitoes in yucatán, mexico, and simulated various vaccine scenarios to evaluate effectiveness under those conditions. this model includes detailed spatial representation of the yucatán population, including the location and mov ... | 2016 | 27227883 |
| temporal dynamics and spatial patterns of aedes aegypti breeding sites, in the context of a dengue control program in tartagal (salta province, argentina). | since 2009, fundación mundo sano has implemented an aedes aegypti surveillance and control program in tartagal city (salta province, argentina). the purpose of this study was to analyze temporal dynamics of ae. aegypti breeding sites spatial distribution, during five years of samplings, and the effect of control actions over vector population dynamics. | 2016 | 27223693 |
| meta-analysis of dengue severity during infection by different dengue virus serotypes in primary and secondary infections. | dengue virus (denv) infection is currently a major cause of morbidity and mortality in the world; it has become more common and virulent over the past half-century and has gained much attention. thus, this review compared the percentage of severe cases of both primary and secondary infections with different serotypes of dengue virus. | 2016 | 27213782 |
| proceedings of the 3rd ipleiria's international health congress : leiria, portugal. 6-7 may 2016. | 2016 | 27409075 | |
| a review of chikungunya virus-induced arthralgia: clinical manifestations, therapeutics, and pathogenesis. | chikungunya virus (chikv) is a mosquito-borne alphavirus that circulates predominantly in tropical and subtropical regions, potentially affecting over 1 billion people. recently, an outbreak began in the western hemisphere and has resulted in over 1.8 million reported suspected cases. infection often results in severe fever, rash and debilitating polyarthralgia lasting weeks to months. additionally, the current literature reports that chikv can result in a severe chronic arthralgia and/or arthri ... | 2016 | 28077980 |
| analysis of chikungunya virus proteins reveals that non-structural proteins nsp2 and nsp3 exhibit rna interference (rnai) suppressor activity. | rnai pathway is an antiviral defence mechanism employed by insects that result in degradation of viral rna thereby curbing infection. several viruses including flaviviruses encode viral suppressors of rnai (vsrs) to counteract the antiviral rnai pathway. till date, no vsr has been reported in alphaviruses. the present study was undertaken to evaluate chikungunya virus (chikv) proteins for rnai suppressor activity. we systematically analyzed all nine chikv proteins for rnai suppressor activity us ... | 2016 | 27901124 |
| diet-induced nutritional stress and pathogen interference in wolbachia-infected aedes aegypti. | the pathogen interference phenotype greatly restricts infection with dengue virus (denv) and other pathogens in wolbachia-infected aedes aegypti, and is a vital component of wolbachia-based mosquito control. critically, the phenotype's causal mechanism is complex and poorly understood, with recent evidence suggesting that the cause may be species specific. to better understand this important phenotype, we investigated the role of diet-induced nutritional stress on interference against denv and t ... | 2016 | 27893736 |
| cross-mating compatibility and competitiveness among aedes albopictus strains from distinct geographic origins - implications for future application of sit programs in the south west indian ocean islands. | the production of large numbers of males needed for a sustainable sterile insect technique (sit) control program requires significant developmental and operational costs. this may constitute a significant economic barrier to the installation of large scale rearing facilities in countries that are undergoing a transition from being largely dependent on insecticide use to be in a position to integrate the sit against aedes albopictus. alternative options available for those countries could be to r ... | 2016 | 27806056 |
| recent chikungunya virus infection in 2 travelers returning from mogadishu, somalia, to italy, 2016. | 2016 | 27513985 | |
| chikungunya virus infection: an update on joint manifestations and management. | the advent of sophisticated diagnostics has enabled the discovery of previously unknown arthropod-borne viruses like chikungunya. this infection has become increasingly prevalent in the last 10 years across the indian ocean and has been brought to media attention by a recent outbreak in the caribbean. the outbreak has been aided by a drastic rise in air travel, allowing infected individuals to transport the virus to previously unaffected regions. in addition, a recently documented viral mutation ... | 2016 | 27824550 |
| in silico study on anti-chikungunya virus activity of hesperetin. | the re-emerging, aedes spp. transmitted chikungunya virus (chikv) has recently caused large outbreaks in a wide geographical distribution of the world including countries in europe and america. though fatalities associated with this self-remitting disease were rarely reported, quality of patients' lives have been severely diminished by polyarthralgia recurrence. neither effective antiviral treatment nor vaccines are available for chikv. our previous in vitro screening showed that hesperetin, a b ... | 2016 | 27812412 |
| use of household cluster investigations to identify factors associated with chikungunya virus infection and frequency of case reporting in puerto rico. | chikungunya virus (chikv) is transmitted by aedes species mosquitoes and is the cause of an acute febrile illness characterized by potentially debilitating arthralgia. after emerging in the caribbean in late 2013, the first locally-acquired case reported to public health authorities in puerto rico occurred in may 2014. during june-august 2014, household-based cluster investigations were conducted to identify factors associated with infection, development of disease, and case reporting. | 2016 | 27764085 |
| chikungunya virus transmission between aedes albopictus and laboratory mice. | chikungunya virus (chikv) is a mosquito-borne alphavirus associated with epidemics of acute and chronic arthritic disease in humans. aedes albopictus has emerged as an important new natural vector for chikv transmission; however, mouse models for studying transmission have not been developed. | 2016 | 27760560 |
| mapping of courses on vector biology and vector-borne diseases systems: time for a worldwide effort. | major emergency efforts are being mounted for each vector-borne disease epidemiological crisis anew, while knowledge about the biology of arthropods vectors is dwindling slowly but continuously, as is the number of field entomologists. the discrepancy between the rates of production of knowledge and its use and need for solving crises is widening, in particular due to the highly differing time spans of the two concurrent processes. a worldwide web based search using multiple key words and search ... | 2016 | 27759770 |
| chikungunya virus in febrile humans and aedes aegypti mosquitoes, yucatan, mexico. | chikungunya virus (chikv) was isolated from 12 febrile humans in yucatan, mexico, in 2015. one patient was co-infected with dengue virus type 1. two additional chikv isolates were obtained from aedes aegypti mosquitoes collected in the homes of patients. phylogenetic analysis showed that the chikv isolates belong to the asian lineage. | 2016 | 27347760 |
| climate change influences potential distribution of infected aedes aegypti co-occurrence with dengue epidemics risk areas in tanzania. | dengue is the second most important vector-borne disease of humans globally after malaria. incidence of dengue infections has dramatically increased recently, potentially due to changing climate. climate projections models predict increases in average annual temperature, precipitation and extreme events in the future. the objective of this study was to assess the effect of changing climate on distribution of dengue vectors in relation to epidemic risk areas in tanzania. | 2016 | 27681327 |
| culex quinquefasciatus from rio de janeiro is not competent to transmit the local zika virus. | the americas have suffered a dramatic epidemic of zika since may in 2015, when zika virus (zikv) was first detected in brazil. mosquitoes belonging to subgenus stegomyia of aedes, particularly aedes aegypti, are considered the primary vectors of zikv. however, the rapid spread of the virus across the continent raised several concerns about the transmission dynamics, especially about potential mosquito vectors. the purpose of this work was to assess the vector competence of the house mosquito cul ... | 2016 | 27598421 |
| knowledge and use of prevention measures for chikungunya virus among visitors - virgin islands national park, 2015. | in june 2014, the mosquito-borne chikungunya virus (chikv) emerged in the u.s. virgin islands (usvi), a location where tourists comprise the majority of the population during peak season (january-april). limited information is available concerning visitors' chikv awareness and prevention measures. | 2016 | 27597388 |
| genetic evidence of expansion by passive transport of aedes (stegomyia) aegypti in eastern argentina. | 2016 | 27583521 | |
| zika virus, a new threat for europe? | since its emergence in 2007 in micronesia and polynesia, the arthropod-borne flavivirus zika virus (zikv) has spread in the americas and the caribbean, following first detection in brazil in may 2015. the risk of zikv emergence in europe increases as imported cases are repeatedly reported. together with chikungunya virus (chikv) and dengue virus (denv), zikv is transmitted by aedes mosquitoes. any countries where these mosquitoes are present could be potential sites for future zikv outbreak. we ... | 2016 | 27505002 |
| expression and characterization of yeast derived chikungunya virus like particles (chik-vlps) and its evaluation as a potential vaccine candidate. | chikungunya virus (chikv) has emerged as a global health concern due to its recent spread in both old and new world. so far, no chikv specific drug or vaccine is licensed for human use. in this study, we report production of chikungunya virus like particles (chik-vlps) using novel yeast expression system (pichia pastoris) and its evaluation as vaccine candidate. the gene encoding structural polyprotein of chikv from a recent epidemic strain was cloned into yeast expression system. the multicopy ... | 2016 | 27399001 |
| juvenile hormone regulation of female reproduction in the common bed bug, cimex lectularius. | to begin studies on reproduction in common bed bug, cimex lectularius, we identified three genes coding for vitellogenin (vg, a protein required for the reproductive success of insects) and studied their hormonal regulation. rna interference studied showed that expression of vg3 gene in the adult females is a prerequisite for successful completion of embryogenesis in the eggs laid by them. juvenile hormone (jh) receptor, methoprene-tolerant (met), steroid receptor coactivator (src) and gataa but ... | 2016 | 27762340 |
| rna interference in moths: mechanisms, applications, and progress. | the vast majority of lepidopterans, about 90%, are moths. some moths, particularly their caterpillars, are major agricultural and forestry pests in many parts of the world. however, some other members of moths, such as the silkworm bombyx mori, are famous for their economic value. fire et al. in 1998 initially found that exogenous double-stranded rna (dsrna) can silence the homolog endogenous mrna in organisms, which is called rna interference (rnai). soon after, the rnai technique proved to be ... | 2016 | 27775569 |
| identification of aedes aegypti long intergenic non-coding rnas and their association with wolbachia and dengue virus infection. | long intergenic non-coding rnas (lincrnas) are appearing as an important class of regulatory rnas with a variety of biological functions. the aim of this study was to identify the lincrna profile in the dengue vector aedes aegypti and evaluate their potential role in host-pathogen interaction. the majority of previous rna-seq transcriptome studies in ae. aegypti have focused on the expression pattern of annotated protein coding genes under different biological conditions. here, we used 35 public ... | 2016 | 27760142 |
| open questions: how does wolbachia do what it does? | a common symbiont of insects, the bacterium wolbachia has been implicated in phenomena as diverse as sex determination, pathogen defence and speciation and is being used in public health programs to prevent mosquitoes transmitting disease. despite decades of research, we know remarkably little about how it exerts its effects. | 2016 | 27760540 |
| perplexing metabolomes in fungal-insect trophic interactions: a terra incognita of mycobiocontrol mechanisms. | the trophic interactions of entomopathogenic fungi in different ecological niches viz., soil, plants, or insect themselves are effectively regulated by their maneuvered metabolomes and the plethora of metabotypes. in this article, we discuss a holistic framework of co-evolutionary metabolomes and metabotypes to model the interactions of biocontrol fungi especially with mycosed insects. conventionally, the studies involving fungal biocontrol mechanisms are reported in the context of much aggrandi ... | 2016 | 27807434 |
| cloning and expression of ecdysone receptor and retinoid x receptor from procambarus clarkii: induction by eyestalk ablation. | ecdysone receptor and retinoid x receptor are key regulators in molting. here, full length ecdysone receptor (pcecr) and retinoid x receptor (pcrxr) cdnas from procambarus clarkii were cloned. full length cdna of pcecr has 2500 bp, encoding 576 amino acid proteins, and full length cdna of pcrxr has 2593 bp, in which a 15 bp and a 204 bp insert/deletion splice variant regions in dna binding domain and hinge domain were identified. the two splice variant regions in pcrxr result four isoforms: pcrx ... | 2016 | 27763563 |
| morphological and transcriptomic analysis of a beetle chemosensory system reveals a gnathal olfactory center. | the red flour beetle tribolium castaneum is an emerging insect model organism representing the largest insect order, coleoptera, which encompasses several serious agricultural and forest pests. despite the ecological and economic importance of beetles, most insect olfaction studies have so far focused on dipteran, lepidopteran, or hymenopteran systems. | 2016 | 27751175 |
| bacillus thuringiensis cyt2aa2 toxin disrupts cell membranes by forming large protein aggregates. | bacillus thuringiensis (bt) cyt2aa2 showed toxicity against dipteran insect larvae and in vitro lysis activity on several cells. it has potential applications in the biological control of insect larvae. although pore-forming and/or detergent-like mechanisms were proposed, the mechanism underlying cytolytic activity remains unclear. analysis of the haemolytic activity of cyt2aa2 with osmotic stabilizers revealed partial toxin inhibition, suggesting a distinctive mechanism from the putative pore f ... | 2016 | 27612497 |
| haemocoel injection of pira1b1 to galleria mellonella larvae leads to disruption of the haemocyte immune functions. | the bacterium photorhabdus luminescens produces a number of insecticidal proteins to kill its larval prey. in this study, we cloned the gene coding for a binary toxin pira1b1 and purified the recombinant protein using affinity chromatography combined with desalination technology. furthermore, the cytotoxicity of the recombinant protein against the haemocytes of galleria mellonella larvae was investigated. we found that the protein had haemocoel insecticidal activity against g. mellonella with an ... | 2016 | 27734915 |
| time-lagging interplay effect and excess risk of meteorological/mosquito parameters and petrochemical gas explosion on dengue incidence. | in kaohsiung, a metropolitan city in taiwan at high risk of dengue epidemic, weather factors combined with an accidental petrochemical gas explosion (pge) may affect mosquito‒human dynamics in 2014. generalized estimating equations with lagged-time poisson regression analyses were used to evaluate the effect of meteorological/mosquito parameters and pge on dengue incidences (2000-2014) in kaohsiung. increased minimum temperatures rendered a 2- and 3-month lagging interactive effect on higher den ... | 2016 | 27733774 |
| expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite coptotermes gestroi. | termites are considered one of the most efficient decomposers of lignocelluloses on earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (cazymes). recently, a set of pro-oxidant, antioxidant, and detoxification enzymes (pad) were also correlated with the metabolism of carbohydrates and lignin in termites. the lower termite coptotermes gestroi is considered the main urban pest in brazil, causing damage to wood constructions. recen ... | 2016 | 27790186 |
| effects of a novel β-lapachone derivative on trypanosoma cruzi: parasite death involving apoptosis, autophagy and necrosis. | natural products comprise valuable sources for new antiparasitic drugs. here we tested the effects of a novel β-lapachone derivative on trypanosoma cruzi parasite survival and proliferation and used microscopy and cytometry techniques to approach the mechanism(s) underlying parasite death. the selectivity index determination indicate that the compound trypanocidal activity was over ten-fold more cytotoxic to epimastigotes than to macrophages or splenocytes. scanning electron microscopy analysis ... | 2016 | 27770751 |
| eukaryotic association module in phage wo genomes from wolbachia. | viruses are trifurcated into eukaryotic, archaeal and bacterial categories. this domain-specific ecology underscores why eukaryotic viruses typically co-opt eukaryotic genes and bacteriophages commonly harbour bacterial genes. however, the presence of bacteriophages in obligate intracellular bacteria of eukaryotes may promote dna transfers between eukaryotes and bacteriophages. here we report a metagenomic analysis of purified bacteriophage wo particles of wolbachia and uncover a eukaryotic asso ... | 2016 | 27727237 |
| infection with plasmodium berghei ookinetes alters protein expression in the brain of anopheles albimanus mosquitoes. | the behaviour of anopheles spp. mosquitoes, vectors for plasmodium parasites, plays a crucial role in the propagation of malaria to humans. consequently, it is important to understand how the behaviour of these mosquitoes is influenced by the interaction between the brain and immunological status. the nervous system is intimately linked to the immune and endocrine systems. there is evidence that the malaria parasite alters the function of these systems upon infecting the mosquito. although there ... | 2016 | 27724938 |
| darwin's finches treat their feathers with a natural repellent. | darwin's finches are highly innovative. recently we recorded for the first time a behavioural innovation in darwin's finches outside the foraging context: individuals of four species rubbed leaves of the endemic tree psidium galapageium on their feathers. we hypothesised that this behaviour serves to repel ectoparasites and tested the repellency of p. galapageium leaf extracts against parasites that negatively affect the fitness of darwin's finches, namely mosquitoes and the invasive hematophago ... | 2016 | 27721475 |
| transcriptome comparison analysis of ostrinia furnacalis in four developmental stages. | the asian corn borer, ostrinia furnacalis, is one of the most destructive pests of maize and causes huge losses in maize yield each year. in order to characterize the different developmental stages, a high-throughput sequencing platform was employed to perform de novo transcriptome assembly and gene expression analysis for the egg, larva, pupa and adult stages. approximately 185 million reads were obtained, trimmed, and assembled into 42,638 unigenes with an average length of 801.94 bp and an n5 ... | 2016 | 27713521 |
| glycoside hydrolases family 20 (gh20) represent putative virulence factors that are shared by animal pathogenic oomycetes, but are absent in phytopathogens. | although interest in animal pathogenic oomycetes is increasing, the molecular basis mediating oomycete-animal relationships remains virtually unknown. crinkler (crn) genes, which have been traditionally associated with the cytotoxic activity displayed by plant pathogenic oomycetes, were recently detected in transcriptome sequences from the entomopathogenic oomycete lagenidium giganteum, suggesting that these genes may represent virulence factors conserved in both animal and plant pathogenic oomy ... | 2016 | 27716041 |
| genetic analysis of aedes aegypti using random amplified polymorphic dna (rapd) markers from dengue outbreaks in pakistan. | keeping in view the havoc situation of dengue fever in pakistan, the current study was designed to demonstrate the genetic variations, gene flow and rate of migration from lahore and faisalabad. | 2016 | 28032107 |
| transcriptome response to elevated atmospheric co2 concentration in the formosan subterranean termite, coptotermes formosanus shiraki (isoptera: rhinotermitidae). | carbon dioxide (co2) is a pervasive chemical stimulus that plays a critical role in insect life, eliciting behavioral and physiological responses across different species. high co2 concentration is a major feature of termite nests, which may be used as a cue for locating their nests. termites also survive under an elevated co2 concentration. however, the mechanism by which elevated co2 concentration influences gene expression in termites is poorly understood. | 2016 | 27761326 |
| how social structures, space, and behaviors shape the spread of infectious diseases using chikungunya as a case study. | whether an individual becomes infected in an infectious disease outbreak depends on many interconnected risk factors, which may relate to characteristics of the individual (e.g., age, sex), his or her close relatives (e.g., household members), or the wider community. studies monitoring individuals in households or schools have helped elucidate the determinants of transmission in small social structures due to advances in statistical modeling; but such an approach has so far largely failed to con ... | 2016 | 27821727 |
| the epidemiological characteristics and dynamic transmission of dengue in china, 2013. | there was a dengue epidemic in several regions of china in 2013. no study has explored the dynamics of dengue transmission between different geographical locations with dengue outbreaks in china. the purpose of the study is to analyze the epidemiological characteristics and to explore the dynamic transmission of dengue in china, 2013. | 2016 | 27820815 |
| a cluster-randomized controlled trial to reduce diarrheal disease and dengue entomological risk factors in rural primary schools in colombia. | as many neglected tropical diseases are co-endemic and have common risk factors, integrated control can efficiently reduce disease burden and relieve resource-strained public health budgets. diarrheal diseases and dengue fever are major global health problems sharing common risk factors in water storage containers. where provision of clean water is inadequate, water storage is crucial. fecal contamination of stored water is a common source of diarrheal illness, but stored water also provides bre ... | 2016 | 27820821 |
| talen-mediated knock-in via non-homologous end joining in the crustacean daphnia magna. | transcription activator-like effector nucleases (talens) are versatile tools that enable the insertion of dna into different organisms. here, we confirmed talen-mediated knock-in via non-homologous end joining in the crustacean daphnia magna, a model organism for ecological and toxicological genomics. we tested two different talens, ey1 talen and ey2 talen, both of which target the eyeless locus. the donor dna plasmid, harbouring the h2b-gfp reporter gene, was designed to contain both talen targ ... | 2016 | 27819301 |
| oral delivery of dsrna by microbes: beyond pest control. | rna interference (rnai) by oral delivery of dsrna in insects has great potential as a tool for integrated pest management (ipm), especially with respect to addressing the need to reduce off-target effect and slow down resistance development to chemical insecticides. employing the natural association existing between insect and yeast, we developed a novel method to enable the knock down of vital genes in the pest insect drosophila suzukii through oral delivery of species-specific dsrna using gene ... | 2016 | 28042376 |
| host plant affects morphometric variation of diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid (acp), diaphorina citri kuwayama, is one of the most serious citrus pests worldwide due to its role as vector of huanglongbing or citrus greening disease. while some optimal plant species for acp oviposition and development have been identified, little is known of the influence of host plants on acp size and shape. our goal was to determine how size and shape of acp wing and body size varies when development occurs on different host plants in a controlled rearing environ ... | 2016 | 27833820 |
| recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus. | lumpy skin disease virus (lsdv) is a capripoxvirus infecting cattle and buffalos. lumpy skin disease (lsd) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). early detection of the virus is crucial to start appropriate outbreak control measures. veterinarians rely on the presence of the characteristic clinical signs of lsd. laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain ... | 2016 | 27806722 |
| genome-wide analysis of differentially expressed microrna in bombyx mori infected with nucleopolyhedrosis virus. | bombyx mori nucleopolyhedrosis virus (bmnpv) is a major pathogen that threatens the growth and sustainability of the sericulture industry. since micrornas (mirnas) have been shown to play important roles in host-pathogen interactions, in this study we investigated the effects of bmnpv infection on silkworm micrornas expression profile. to achieve this, we constructed and deep-sequenced two small rna libraries generated from bmnpv infected and un-infected larvae. the results revealed that 38 silk ... | 2016 | 27806111 |
| the seminal fluid proteome of the polyandrous red junglefowl offers insights into the molecular basis of fertility, reproductive ageing and domestication. | seminal fluid proteins (sfps) are emerging as fundamental contributors to sexual selection given their role in post-mating reproductive events, particularly in polyandrous species where the ejaculates of different males compete for fertilisation. sfp identification however remains taxonomically limited and little is known about avian sfps, despite extensive work on sexual selection in birds. we characterize the sf proteome of the polyandrous red junglefowl, gallus gallus, the wild species that g ... | 2016 | 27804984 |
| turmeric powder and its derivatives from curcuma longa rhizomes: insecticidal effects on cabbage looper and the role of synergists. | curcuma longa has well-known insecticidal and repellent effects on insect pests, but its impact on trichoplusia ni is unknown. in this study, the compound ar-turmerone, extracted and purified from c. longa rhizomes, was identified, and its insecticidal effects, along with turmeric powder, curcuminoid pigments and crude essential oil were evaluated against this important agricultural pest. the role of natural (sesamol and piperonal) and synthetic [piperonyl butoxide (pbo)] synergists under labora ... | 2016 | 27804972 |
| sodium channel mutations and pyrethroid resistance in aedes aegypti. | pyrethroid insecticides are widely used to control insect pests and human disease vectors. voltage-gated sodium channels are the primary targets of pyrethroid insecticides. mutations in the sodium channel have been shown to be responsible for pyrethroid resistance, known as knockdown resistance (kdr), in various insects including mosquitoes. in aedes aegypti mosquitoes, the principal urban vectors of dengue, zika, and yellow fever viruses, multiple single nucleotide polymorphisms in the sodium c ... | 2016 | 27809228 |
| filarial infection influences mosquito behaviour and fecundity. | understanding vector-parasite interactions is increasingly important as we move towards the endpoint goals set by the global programme for the elimination of lymphatic filariasis (gpelf), as interaction dynamics may change with reduced transmission pressure. elimination models used to predict programmatic endpoints include parameters for vector-specific transmission dynamics, despite the fact that our knowledge of the host-seeking behaviour of filariasis infected mosquitoes is lacking. we observ ... | 2016 | 27796352 |
| cgdb: a database of circadian genes in eukaryotes. | we report a database of circadian genes in eukaryotes (cgdb, http://cgdb.biocuckoo.org), containing ∼73 000 circadian-related genes in 68 animals, 39 plants and 41 fungi. circadian rhythm is ∼24 h rhythm in behavioral and physiological processes that exists in almost all organisms on the earth. defects in the circadian system are highly associated with a number of diseases such as cancers. although several databases have been established for rhythmically expressed genes, a comprehensive database ... | 2016 | 27789706 |
| conserved chemosensory proteins in the proboscis and eyes of lepidoptera. | odorant-binding proteins (obps) and chemosensory proteins (csps) are endowed with several different functions besides being carriers for pheromones and odorants. based on a previous report of a csp acting as surfactant in the proboscis of the moth helicoverpa armigera, we revealed the presence of orthologue proteins in two other moths plutella xylostella and chilo suppressalis, as well as two butterflies papilio machaon and pieris rapae, using immunodetection and proteomic analysis. the unusual ... | 2016 | 27877091 |
| spermatogenesis drives rapid gene creation and masculinization of the x chromosome in stalk-eyed flies (diopsidae). | throughout their evolutionary history, genomes acquire new genetic material that facilitates phenotypic innovation and diversification. developmental processes associated with reproduction are particularly likely to involve novel genes. abundant gene creation impacts the evolution of chromosomal gene content and general regulatory mechanisms such as dosage compensation. numerous studies in model organisms have found complex and, at times contradictory, relationships among these genomic attribute ... | 2016 | 26951781 |
| finding aedes aegypti in a natural breeding site in an urban zone, sao paulo, southeastern brazil. | this is the description of how nine aedes aegypti larvae were found in a natural breeding site in the pinheiros neighborhood, city of sao paulo, sp, southeastern brazil. the record was conducted in december 2014, during an entomological surveillance program of dengue virus vectors, with an active search of potential breeding sites, either artificial or natural. findingae. aegypti larvae in a tree hole shows this species' ability to use both artificial and natural environments as breeding sites a ... | 2016 | 26982959 |
| evaluation of the activity of the essential oil from an ornamental flower against aedes aegypti: electrophysiology, molecular dynamics and behavioral assays. | dengue fever has spread worldwide and affects millions of people every year in tropical and subtropical regions of africa, asia, europe and america. since there is no effective vaccine against the dengue virus, prevention of disease transmission depends entirely on regulating the vector (aedes aegypti) or interrupting human-vector contact. the aim of this study was to assess the oviposition deterrent activity of essential oils of three cultivars of torch ginger (etlingera elatior, zingiberaceae) ... | 2016 | 26927837 |
| nutritional signaling regulates vitellogenin synthesis and egg development through juvenile hormone in nilaparvata lugens (stål). | insect female reproduction which comprises the synthesis of vitellogenein (vg) in the fat body and its incorporation into developing oocytes, needs a large amount of energy and food resources. our previous studies found that juvenile hormone (jh) regulates vitellogenesis in the brown planthopper, nilaparvata lugens. here, we report on the role of jh in nutrient-regulated vg synthesis and egg development. we first cloned the genes coding for juvenile hormone acid methyltransferase (jhamt) which i ... | 2016 | 26927076 |
| evolution of the assassin's arms: insights from a phylogeny of combined transcriptomic and ribosomal dna data (heteroptera: reduvioidea). | assassin bugs (reduvioidea) are one of the most diverse (>7,000 spp.) lineages of predatory animals and have evolved an astounding diversity of raptorial leg modifications for handling prey. the evolution of these modifications is not well understood due to the lack of a robust phylogeny, especially at deeper nodes. we here utilize refined data from transcriptomes (370 loci) to stabilize the backbone phylogeny of reduvioidea, revealing the position of major clades (e.g., the chagas disease vecto ... | 2016 | 26916580 |
| rna sequencing to characterize transcriptional changes of sexual maturation and mating in the female oriental fruit fly bactrocera dorsalis. | female reproductive potential plays a significant role in the survival and stability of species, and sexual maturation and mating processes are crucial. however, our knowledge of the reproductive genes involved in sexual maturation and mating has been largely limited to model organisms. the oriental fruit fly bactrocera dorsalis is a highly invasive agricultural pest, known to cause major economic losses; thus, it is of great value to understand the transcriptional changes involved in sexual mat ... | 2016 | 26946038 |
| identification of autophagy in the pine wood nematode bursaphelenchus xylophilus and the molecular characterization and functional analysis of two novel autophagy-related genes, bxatg1 and bxatg8. | the pine wood nematode, bursaphelenchus xylophilus, causes huge economic losses in pine forests, has a complex life cycle, and shows the remarkable ability to survive under unfavorable and changing environmental conditions. this ability may be related to autophagy, which is still poorly understood in b. xylophilus and no autophagy-related genes have been previously characterized. in this study, transmission electron microscopy was used to confirm that autophagy exists in b. xylophilus. the full- ... | 2016 | 26950119 |
| distribution of voltage-gated sodium channel (nav) alleles among the aedes aegypti populations in central java province and its association with resistance to pyrethroid insecticides. | the emergence of insecticide resistant aedes aegypti mosquitoes has hampered dengue control efforts. who susceptibility tests, using several pyrethroid compounds, were conducted on ae. aegypti larvae that were collected and raised to adulthood from semarang, surakarta, kudus and jepara in java. the aanav gene fragment encompassing kdr polymorphic sites from both susceptible and resistant mosquitoes was amplified, and polymorphisms were associated with the resistant phenotype. the insecticide sus ... | 2016 | 26939002 |
| spatial and temporal epidemiology of lumpy skin disease in the middle east, 2012-2015. | lumpy skin disease virus (lsdv) is an infectious disease of cattle that can have severe economic implications. new lsd outbreaks are currently circulating in the middle east (me). since 2012, severe outbreaks were reported in cattle across the region. characterizing the spatial and temporal dynamics of lsdv in cattle populations is prerequisite for guiding successful surveillance and control efforts at a regional level in the me. here, we aim to model the ecological niche of lsdv and identify ep ... | 2016 | 26973845 |
| multiple evolutionary selections involved in synonymous codon usages in the streptococcus agalactiae genome. | streptococcus agalactiae is an important human and animal pathogen. to better understand the genetic features and evolution of s. agalactiae, multiple factors influencing synonymous codon usage patterns in s. agalactiae were analyzed in this study. a- and u-ending rich codons were used in s. agalactiae function genes through the overall codon usage analysis, indicating that adenine (a)/thymine (t) compositional constraints might contribute an important role to the synonymous codon usage pattern. ... | 2016 | 26927064 |
| a disease around the corner. | 2016 | 26981335 | |
| a primary screening and applying of plant volatiles as repellents to control whitefly bemisia tabaci (gennadius) on tomato. | with the goal of finding a new way to reduce population densities of bemisia tabaci biotype q in greenhouses, seven repellent volatile chemicals and their combinations were screened. the mixture of dlco (d-limonene, citral and olive oil (63:7:30)) had a better cost performance(sc50 = 22.59 mg/ml)to repel whiteflies from settling than the other mixtures or single chemicals. in the greenhouse, in both the choice test and the no-choice tests, the number of adult whiteflies that settled on 1% dlco-t ... | 2016 | 26907368 |
| changes in the physicochemical properties of piperine/β-cyclodextrin due to the formation of inclusion complexes. | piperine (pp) is a pungent component in black pepper that possesses useful biological activities; however it is practically insoluble in water. the aim of the current study was to prepare a coground mixture (gm) of pp and β-cyclodextrin (βcd) (molar ratio of pp/βcd = 1/1) and subsequently evaluate the solubility of pp and physicochemical properties of the gm. dsc thermal behavior of the gm showed the absence of melting peak of piperine. pxrd profile of the gm exhibited halo pattern and no charac ... | 2016 | 26998357 |
| insight into the mode of action of haedoxan a from phryma leptostachya. | haedoxan a (ha) is a major active ingredient in the herbaceous perennial plant lopseed (phryma leptostachya l.), which is used as a natural insecticide against insect pests in east asia. here, we report that ha delayed the decay rate of evoked excitatory junctional potentials (ejps) and increased the frequency of miniature ejps (mejps) on the drosophila neuromuscular junction. ha also caused a significant hyperpolarizing shift of the voltage dependence of fast inactivation of insect sodium chann ... | 2016 | 26907348 |
| chemical diversity and biological properties of secondary metabolites from sea hares of aplysia genus. | the marine environment is an important source of structurally-diverse and biologically-active secondary metabolites. during the last two decades, thousands of compounds were discovered in marine organisms, several of them having inspired the development of new classes of therapeutic agents. marine mollusks constitute a successful phyla in the discovery of new marine natural products (mnps). over a 50-year period from 1963, 116 genera of mollusks contributed innumerous compounds, aplysia being th ... | 2016 | 26907303 |
| developing a time series predictive model for dengue in zhongshan, china based on weather and guangzhou dengue surveillance data. | dengue is a re-emerging infectious disease of humans, rapidly growing from endemic areas to dengue-free regions due to favorable conditions. in recent decades, guangzhou has again suffered from several big outbreaks of dengue; as have its neighboring cities. this study aims to examine the impact of dengue epidemics in guangzhou, china, and to develop a predictive model for zhongshan based on local weather conditions and guangzhou dengue surveillance information. | 2016 | 26894570 |
| differential juvenile hormone variations in scale insect extreme sexual dimorphism. | scale insects have evolved extreme sexual dimorphism, as demonstrated by sedentary juvenile-like females and ephemeral winged males. this dimorphism is established during the post-embryonic development; however, the underlying regulatory mechanisms have not yet been examined. we herein assessed the role of juvenile hormone (jh) on the diverging developmental pathways occurring in the male and female japanese mealybug planococcus kraunhiae (kuwana). we provide, for the first time, detailed gene e ... | 2016 | 26894583 |
| transcriptome analysis and discovery of genes relevant to development in bradysia odoriphaga at three developmental stages. | bradysia odoriphaga (diptera: sciaridae) is the most important pest of chinese chive (allium tuberosum) in asia; however, the molecular genetics are poorly understood. to explore the molecular biological mechanism of development, illumina sequencing and de novo assembly were performed in the third-instar, fourth-instar, and pupal b. odoriphaga. the study resulted in 16.2 gb of clean data and 47,578 unigenes (≥125 bp) contained in 7,632,430 contigs, 46.21% of which were annotated from non-redunda ... | 2016 | 26891450 |
| establishment of a wolbachia superinfection in aedes aegypti mosquitoes as a potential approach for future resistance management. | wolbachia pipientis is an endosymbiotic bacterium estimated to chronically infect between 40-75% of all arthropod species. aedes aegypti, the principle mosquito vector of dengue virus (denv), is not a natural host of wolbachia. the transinfection of wolbachia strains such as walbb, wmel and wmelpop-cla into ae. aegypti has been shown to significantly reduce the vector competence of this mosquito for a range of human pathogens in the laboratory. this has led to wmel-transinfected ae. aegypti curr ... | 2016 | 26891349 |
| deep rna-seq profile reveals biodiversity, plant-microbe interactions and a large family of nbs-lrr resistance genes in walnut (juglans regia) tissues. | deep rna-seq profiling, a revolutionary method used for quantifying transcriptional levels, often includes non-specific transcripts from other co-existing organisms in spite of stringent protocols. using the recently published walnut genome sequence as a filter, we present a broad analysis of the rna-seq derived transcriptome profiles obtained from twenty different tissues to extract the biodiversity and possible plant-microbe interactions in the walnut ecosystem in california. since the residua ... | 2016 | 26883051 |
| large-scale modelling of the environmentally-driven population dynamics of temperate aedes albopictus (skuse). | the asian tiger mosquito, aedes albopictus, is a highly invasive vector species. it is a proven vector of dengue and chikungunya viruses, with the potential to host a further 24 arboviruses. it has recently expanded its geographical range, threatening many countries in the middle east, mediterranean, europe and north america. here, we investigate the theoretical limitations of its range expansion by developing an environmentally-driven mathematical model of its population dynamics. we focus on t ... | 2016 | 26871447 |
| climate and the timing of imported cases as determinants of the dengue outbreak in guangzhou, 2014: evidence from a mathematical model. | as the world's fastest spreading vector-borne disease, dengue was estimated to infect more than 390 million people in 2010, a 30-fold increase in the past half century. although considered to be a non-endemic country, mainland china had 55,114 reported dengue cases from 2005 to 2014, of which 47,056 occurred in 2014. furthermore, 94% of the indigenous cases in this time period were reported in guangdong province, 83% of which were in guangzhou city. in order to determine the possible determinant ... | 2016 | 26863623 |