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efficacy of a combined nanoparticulate/calcium hydroxide root canal medication on elimination of enterococcus faecalis.the purpose of this study was to evaluate the efficacy of ca(oh)2 with or without a silver nanoparticle suspension to eliminate enterococcus faecalis from root canals. a total of 66 extracted human single-rooted teeth contaminated with e. faecalis were treated with 10% ca(oh)2 alone, ca(oh)2 with nanosilver or sterile water (as a negative control). samples were obtained with paper points and gates-glidden burs at 1 and 7 days after root canal preparation and the number of colony-forming units (c ...201425244219
[development of an enterococcus faecalis periapical biofilm model for in vitro morphological study].this study aims to develop and observe a model system of the periapical biofilm structure of enterococcus faecalis (e. faecalis).201425241529
plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: an in vitro study.autologous platelet concentrates are successfully adopted in a variety of medical fields to stimulate bone and soft tissue regeneration. the rationale for their use consists in the delivery of a wide range of platelet-derived bioactive molecules that promotes wound healing. in addition, antimicrobial properties of platelet concentrates have been pointed out. in this study, the effect of the platelet concentration, of the activation step and of the presence of plasmatic components on the antimicr ...201425232963
ebpa vaccine antibodies block binding of enterococcus faecalis to fibrinogen to prevent catheter-associated bladder infection in mice.enterococci bacteria are a frequent cause of catheter-associated urinary tract infections, the most common type of hospital-acquired infection. treatment has become increasingly challenging because of the emergence of multiantibiotic-resistant enterococcal strains and their ability to form biofilms on catheters. we identified and targeted a critical step in biofilm formation and developed a vaccine that prevents catheter-associated urinary tract infections in mice. in the murine model, formation ...201425232179
highly diverse microbiota in dental root canals in cases of apical periodontitis (data of illumina sequencing).chronic apical periodontitis (cap) is a frequent condition that has a considerable effect on a patient's quality of life. we aimed to reveal root canal microbial communities in antibiotic-naive patients by applying illumina sequencing (illumina inc, san diego, ca).201425227214
antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials propolis and aloe vera against enterococcus faecalis: an ex vivo study.the aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, propolis and aloe vera on enterococcus faecalis in deep dentin.201425225560
carbamidocyclophanes f and g with anti-mycobacterium tuberculosis activity from the cultured freshwater cyanobacterium nostoc sp.two new (1 and 2) and three known (3-5) carbamidocyclophanes were isolated from a cultured freshwater cyanobacterium nostoc sp. (uic 10274) obtained from a sample collected at des plaines, illinois. their planar structures and stereoconfigurations were determined by extensive spectroscopic analysis including 1d/2d nmr experiments, hresims as well as cd spectroscopy. carbamidocyclophane f (1) showed potent anti-mycobacterium tuberculosis activity in the microplate alamar blue assay and low-oxygen ...201425225453
antibacterial properties of root canal lubricants: a comparison with commonly used irrigants.the aim was to assess in vitro the antibacterial activity of 10 root canal lubricants. k-y jelly personal lubricant, rc-prep, file-eze, file-rite, endoprep gel, endosure prep crème 15%, prep-rite, glyde, slickgel es and alpha glide were selected and compared in their antimicrobial properties to seven irrigants. serial dilutions of each agent in tryptic soy broth were inoculated with either enterococcus faecalis or pseudomonas aeruginosa and incubated at 37c for 24 h. during incubation bacterial ...201425223525
control of listeria monocytogenes in fresh cheese using protective lactic acid bacteria.in the past years, there has been a particular focus on the application of bacteriocins produced by lactic acid bacteria (lab) in controlling the growth of pathogenic bacteria in foods. the aim of this study was to select lab strains with antimicrobial activity, previously isolated from a traditional azorean artisanal cheese (pico cheese), in order to identify those with the greatest potential in reducing listeria monocytogenes in fresh cheese. eight bacteriocin producer strains identified as la ...201425222327
development and evaluation of a quadruplex taq man real-time pcr assay for simultaneous detection of clinical isolates of enterococcus faecalis, enterococcus faecium and their vana and vanb genotypes.we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci.201425848524
antimicrobial activity of root canal irrigants associated with cetrimide against biofilm and planktonic enterococcus faecalis.to evaluate the antibacterial activity of sodium hypochlorite (naocl) and chlorhexidine (chx) alone or associated with cetrimide (ctr), and qmix against biofilm and planktonic enterococcus faecalis (e. faecalis) [american type culture collection (atcc) 29212].201425707833
vancomycin-resistant enteroccus faecium and enterococcus faecalis isolated from education hospital of iran.enterococci are opportunistic pathogens which represent one of the leading agents of nosocomial infections, especially urinary tract infections (uti) in hospitalized patients. the aim of the present study was to determine the resistance pattern and the type of resistance genes in vancomycin-resistant enterococcus isolated from an educational hospital in iran.201425705299
[inferior vena cava thrombosis and endocarditis in a premature patient: case report].thrombosis of the inferior vena cava represents a significant percentage of all venous thrombosis that take place during the neonatal period, generally associated with risk factors such as the use of central venous catheter. the incidence of bacterial endocarditis in preterm infants is low.201425697617
antimicrobial activity of extracts and isoquinoline alkaloids of selected papaveraceae plants.alkaloidal extracts of seven selected plants of the family papaveraceae were studied with respect to their activity against six strains of pathogenic bacteria and their alkaloidal fingerprint. twenty-four alkaloids were determined by gc/ms, and twenty of them identified from their mass spectra, retention times and retention indexes. in the antibacterial assay, three gram-positive (enterorococcus faecalis, staphylococcus aureus and s. hyicus), and three gram-negative (escherichia coli, proteus mi ...201425632464
efficacy of contemporary and novel intracanal medicaments against enterococcus faecalis.to compare the antibacterial activity of propolis (30% in methyl cellulose), curcumin (2.5mg/ml of methyl cellulose), 2% chlorhexidine gel (chx), 2% metronidazole gel (mz) and a mixture of 2% chx and 2% mz against enterococcus faecalis in vitro. calcium hydroxide served as the control.201425631726
an in vivo comparison of antimicrobial efficacy of sodium hypochlorite and biopure mtad™ against enterococcus faecalis in primary teeth: a qpcr study.biopure mtad™, a new root canal irrigant has shown promising results against the most common resistant microorganism, e. faecalis, in permanent teeth. however, there is lack of studies comparing its antimicrobial effectiveness with naocl in primary teeth. the purpose of this study was to compare the in vivo antimicrobial efficacy of naocl 2.5% and biopure mtad™ against e. faecalis in primary teeth.201425631723
diagnostic and therapeutic utility of transrectal ultrasound in urological office prostatic abscess management: a short report from a single urologic center.prostatic abscess (pa) is an infrequent condition in the modern antibiotic era. the everyday use of transrectal ultrasound (trus) during diagnostic work-up and the widespread recurrence to prostatic biopsies may lead to an increase of pa diagnosis. in this short report we analyze the patients characteristics and the management of seven recent cases of pa diagnosed in our institution.201425641468
antibacterial effect of all-in-one self-etch adhesives on enterococcus faecalis.background and aims. the aim of this study was to evaluate the antibacterial activity of one-step self-etch adhesives on enterococcus faecalis on days 1, 7 and 14 with the use of modified direct contact test. materials and methods. the modified directcontact test was used to evaluate the antibacterial effect of adper easy one, bond force, clearfil s3 bond, futurabond m, g-bond, ibond and optibond all-in-one adhesives on enterococcus faecalis after aging the samples in phosphate-buffered saline f ...201425587384
assessment of photodynamic therapy (pdt) in disinfection of deeper dentinal tubules in a root canal system: an in vitro study.the success of endodontic treatment therapy depends on how well we eliminate pathogenic microflora from the root canal system as micro organism as the major cause of root canal infection. conventional root canal treatment can fail if microorganisms cannot be removed sufficiently by thorough cleaning, shaping of root canal. newer modalities such as photodynamic therapy are being tried now a days for disinfection of root canals. aim & objectives: the basic aim of this study was assessment of the a ...201425584321
an in vitro evaluation of antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin.to evaluate in vitro the antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin.201425584319
risk factors analysis for occurrence of asymptomatic bacteriuria after endourological procedures.endourological procedures are performed according to the principles of aseptic techniques, jet still in certain number of patients urinary tract infections may occur. considering the risk of urinary tract infection, there is no unique opinion about the prophylactic use of antibiotics in endourological procedures.201425568546
susceptibility of glycopeptide-resistant enterococci to linezolid, quinupristin/dalfopristin, tigecycline and daptomycin in a tertiary greek hospital.we investigated the antibiotic susceptibility of glycopeptide-resistant enterococci (gre). seventy consecutive gre were tested. sixty-two isolates were identified as enterococcus faecium (88.6%), and 8 (11.4%) as enterococcus faecalis. all strains were susceptible to linezolid and daptomycin, while 17.1% (12/70) and 11.4% (8/70) were resistant to quinupristin/dalfopristin (qd) and tigecycline, respectively. all e. faecalis isolates were resistant to qd, while 4 of 62 (6.5%) e. faecium isolates w ...201425566405
nanobioarchitectures based on chlorophyll photopigment, artificial lipid bilayers and carbon nanotubes.in the last decade, building biohybrid materials has gained considerable interest in the field of nanotechnology. this paper describes an original design for bionanoarchitectures with interesting properties and potential bioapplications. multilamellar lipid vesicles (obtained by hydration of a dipalmitoyl phosphatidylcholine thin film) with and without cholesterol were labelled with a natural photopigment (chlorophyll a), which functioned as a sensor to detect modifications in the artificial lip ...201425551059
transcriptome analysis of enterococcus faecalis during mammalian infection shows cells undergo adaptation and exist in a stringent response state.as both a commensal and a major cause of healthcare-associated infections in humans, enterococcus faecalis is a remarkably adaptable organism. we investigated how e. faecalis adapts in a mammalian host as a pathogen by characterizing changes in the transcriptome during infection in a rabbit model of subdermal abscess formation using transcriptional microarrays. the microarray experiments detected 222 and 291 differentially regulated genes in e. faecalis og1rf at two and eight hours after subderm ...201425545155
identification and antimicrobial resistance of enterococcus spp. isolated from the river and coastal waters in northern iran.as fecal streptococci commonly inhabit the intestinal tract of humans and warm blooded animals, and daily detection of all pathogenic bacteria in coastal water is not practical, thus these bacteria are used to detect the fecal contamination of water. the present study examined the presence and the antibiotic resistance patterns of enterococcus spp. isolated from the babolrud river in babol and coastal waters in babolsar. seventy samples of water were collected in various regions of the babolrud ...201425525617
evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis.photodynamic therapy (pdt) has been proven to be effective in disinfecting root canals. the aim of this present study was to evaluate the effects of pdt on the viability of enterococcus faecalis using methylene blue (mb) and malachite green (mg) as photosensitizers. solutions containing e. faecalis (atcc 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with mb and mg. samples were individually irradiated by the diode laser at a distance of 1mm for ...201425523956
prevalence of enterococcus faecalis and porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: a molecular study.because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome.201425518530
[clinical efficacy of the antimicrobial drug furamag in nosocomial urinary tract infections].to evaluate the efficacy of the combined drug furamag (furasidine potassium and magnesium hydroxycarbonate) in combination with the third-generation cephalosporin cefotaxime versus cephalosporin monotherapy for nosocomial urinary tract infections (nuti).201425509895
[enterococcal infections: from simple to most complex].enterococci are microorganisms with a remar- kable ability to adapt to their environment. two species have a significant clinical implication, enterococcus faecalis and enterococcus faecium. the risk factors for colonization and infection must be recognized, including prior treatment with antibiotics such as cephalosporins or quinolones. because of their native resistance to several classes of antibiotics and the increase of acquired resistance to penicillins, the initial empiric treatment of a ...201425507892
disinfection of gutta-percha cones using three reagents and their residual effects.the purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and mtad in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure.201425506147
community-acquired methicillin-resistant staphylococcus epidermidis pyelonephritis in a child: a case report.staphylococcus epidermidis is currently the most frequent pathogen of opportunistic and nosocomial infections worldwide. most cases of staphylococcus epidermidis infections are associated with indwelling medical devices and/or immunocompromised conditions. community-acquired urinary tract infections are rare, particularly among pediatric populations, and clinicians often do not consider staphylococcus epidermidis as a uropathogen.201425488491
efficacy of antimicrobial photodynamic therapy for root canals infected with enterococcus faecalis.effective decontamination of root canal systems is a constant concern in clinical practice. in this article, we compare the performance of photodynamic therapy (pdt) and sodium hypochlorite (naocl) in reducing the amount of enterococcus faecalis in root canals. relevant studies were identified by searching electronic databases, including web of science, pubmed, bvs (medline, scielo, lilacs and bbo), scopus, and cochrane, and by manually searching the references of identified studies. the terms u ...201425500925
in vitro activity of amazon plant extracts against enterococcus faecalis.previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from brazilian forests' plants. in the present study, these extracts were subjected to microdilution broth assay (mdba) and disk diffusion assay (dda) using planktonic enterococcus faecalis atcc(®) 29212™ and were submitted to phytochemical analysis in tlc and hplc. three extracts obtained from ipomoea alba (mic < 40 μg/ml), diclinanona calycina (mic ≤ 40 μg/ml) and moronobea coccinea (40 ...201425477906
an agmatine-inducible system for the expression of recombinant proteins in enterococcus faecalis.scientific interest in enterococcus faecalis has increased greatly over recent decades. some strains are involved in food fermentation and offer health benefits, whereas others are vancomycin-resistant and cause infections that are difficult to treat. the limited availability of vectors able to express cloned genes efficiently in e. faecalis has hindered biotechnological studies on the bacterium's regulatory and pathogenicity-related genes. the agmatine deiminase (agdi) pathway of e. faecalis, i ...201425471381
in-vitro screening of malaysian honey from different floral sources for antibacterial activity on human pathogenic bacteria.different researches on therapeutic effects of honey have been conducted in different regions; however the study on the potential antibacterial activity of malaysian honey is still limited. in this study, antibacterial activities of different monofloral honey samples were tested against several common human pathogenic bacteria.201425435614
assessment of the in vitro synergy of daptomycin plus linezolid against multidrug-resistant enterococci.the widespread incidence of enterococci resistant to ampicillin, vancomycin and aminoglycosides, the first-line anti-enterococcal antibiotics, has made the treatment of severe enterococcal infections difficult and alternatives should be explored. we investigated the activity of daptomycin combined with linezolid against three enterococcus faecalis and four enterococcus faecium strains resistant to standard drugs used for therapy. minimum inhibitory concentrations (mics) were determined by the br ...201427873692
vancomycin-resistant enterococci: troublemaker of the 21st century.the emergence of multidrug-resistant and vancomycin-resistant enterococci during the last decade has made it difficult to treat nosocomial infections. although various enterococcal species have been identified, only two (enterococcus faecalis and enterococcus faecium) are responsible for the majority of human infections. vancomycin is an important therapeutic alternative against multidrug-resistant enterococci but is associated with a poor prognosis. resistance to vancomycin dramatically reduces ...201427873678
effect of zirconium oxide and zinc oxide nanoparticles on physicochemical properties and antibiofilm activity of a calcium silicate-based material.the aim of the present study was to evaluate the antibiofilm activity against enterococcus faecalis, compressive strength. and radiopacity of portland cement (pc) added to zirconium oxide (zro2), as radiopacifier, with or without nanoparticulated zinc oxide (zno). the following experimental materials were evaluated: pc, pc + zro2, pc + zro2 + zno (5%), and pc + zro2 + zno (10%). antibiofilm activity was analyzed by using direct contact test (dct) on enterococcus faecalis biofilm, for 5 h or 15 h ...201425431798
penicillin-resistant, ampicillin-susceptible enterococcus faecalis of hospital origin: pbp4 gene polymorphism and genetic diversity.despite the spread of penicillin-resistant, ampicillin-susceptible enterococcus faecalis (prasef) isolates in diverse countries, the mechanisms leading to this unusual resistance phenotype have not yet been investigated. the aim of this study was to evaluate whether polymorphism in the pbp4 gene is associated with penicillin resistance in prasef isolates and to determine their genetic diversity. e. faecalis isolates were recovered from different clinical specimens of hospitalized patients from f ...201425445645
[enterococcal infections: from simple to most complex].enterococci are microorganisms with a remar- kable ability to adapt to their environment. two species have a significant clinical implication, enterococcus faecalis and enterococcus faecium. the risk factors for colonization and infection must be recognized, including prior treatment with antibiotics such as cephalosporins or quinolones. because of their native resistance to several classes of antibiotics and the increase of acquired resistance to penicillins, the initial empiric treatment of a ...201425438375
in vitro activity of heather [calluna vulgaris (l.) hull] extracts on selected urinary tract pathogens.calluna vulgaris l. hull (ericaceae) has been used for treatment of urinary tract infections in traditional medicine. in this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of escherichia coli, enterococcus faecalis and proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) were determined. the concentrations of total phenols ...201425428676
[postcataract endophthalmitis caused by enterococcus faecalis].enterococcus faecalis is a major endophthalmitis-causing pathogen and often causes significant visual impairment. we investigated the clinical background, treatment and the visual outcome of patients with e. faecalis endophthalmitis after cataract surgery.201424505932
chemical composition, antibacterial and cytotoxic activities of the essential oil from the flowers of tunisian convolvulus althaeoides l.this study describes the chemical composition and evaluates the antibacterial and the cytotoxic effects of the essential oil from the flowers of convolvulus althaeoides. its chemical composition, determined by gc and gc-ms, is reported for the first time. a total of 24 compounds, accounting for 95.5% of the total oil, have been identified. the oil was characterised by a high proportion of sesquiterpene hydrocarbons (36.3%), followed by oxygenated sesquiterpenes (34.7%) and oxygenated monoterpene ...201424498906
the structure and competitive substrate inhibition of dihydrofolate reductase from enterococcus faecalis reveal restrictions to cofactor docking.we are addressing bacterial resistance to antibiotics by repurposing a well-established classic antimicrobial target, the dihydrofolate reductase (dhfr) enzyme. in this work, we have focused on enterococcus faecalis, a nosocomial pathogen that frequently harbors antibiotic resistance determinants leading to complicated and difficult-to-treat infections. an inhibitor series with a hydrophobic dihydrophthalazine heterocycle was designed from the anti-folate trimethoprim. we have examined the poten ...201424495113
metagenomic analysis of the stool microbiome in patients receiving allogeneic stem cell transplantation: loss of diversity is associated with use of systemic antibiotics and more pronounced in gastrointestinal graft-versus-host disease.next-generation sequencing of the hypervariable v3 region of the 16s rrna gene isolated from serial stool specimens collected from 31 patients receiving allogeneic stem cell transplantation (sct) was performed to elucidate variations in the composition of the intestinal microbiome in the course of allogeneic sct. metagenomic analysis was complemented by strain-specific enterococcal pcr and indirect assessment of bacterial load by liquid chromatography-tandem mass spectrometry of urinary indoxyl ...201424492144
γ-alkylidene-γ-lactones and isobutylpyrrol-2(5h)-ones analogues to rubrolides as inhibitors of biofilm formation by gram-positive and gram-negative bacteria.several molecules have been discovered that interfere with formation of bacterial biofilms, opening a new strategy for the development of more efficient treatments in case of antibiotic resistant bacteria. amongst the most active compounds are some natural brominated furanones from marine algae delisea pulchra that have proven to be able to control pathogenic biofilms. we have recently reported that some rubrolide analogues are able to inhibit biofilm formation of enterococcus faecalis. in the p ...201424484899
vellozia flavicans mart. ex schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by bothrops jararacussu venom.snakebite is a significant public health issue in tropical countries. in brazil, some of the most common snake envenomations are from bothrops. bothrops bites trigger local and systemic effects including edema, pain, erythema, cyanosis, infections, and necrosis. vellozia flavicans is a plant from the brazilian "cerrado" (savanna) that is popularly used as an anti-inflammatory medicine. since inflammation develops quickly after bothrops bites, which can lead to infection, the aim of the present s ...201424507387
antimicrobial and enzyme inhibitory activities of the constituents of plectranthus madagascariensis (pers.) benth.plectranthus madagascariensis is used as a traditional medicine in southern africa. in search of compounds and activities supporting the medicinal use, the chemical profile of the methanolic extract was studied by high-performance liquid chromatography with diode array detection (hplc-dad). four major constituents were isolated and identified as rosmarinic acid (1), 7β,6β-dihydroxyroyleanone (2), 7β-acetoxy-6β-hydroxyroyleanone (3) and coleon u quinone (4). the two abietane diterpenoids (2 and 3 ...201424506206
antimicrobial activity, acute toxicity and cytoprotective effect of crassocephalum vitellinum (benth.) s. moore extract in a rat ethanol-hcl gastric ulcer model.a decoction of crassocephallum vitellinum (benth.) s. moore (asteraceae) is used in kagera region to treat peptic ulcers. this study seeks to evaluate an aqueous ethanol extract of aerial parts of the plant for safety and efficacy.201424552147
identification of a conserved branched rna structure that functions as a factor-independent terminator.anti-q is a small rna encoded on pcf10, an antibiotic resistance plasmid of enterococcus faecalis, which negatively regulates conjugation of the plasmid. in this study we sought to understand how anti-q is generated relative to larger transcripts of the same operon. we found that anti-q folds into a branched structure that functions as a factor-independent terminator. in vitro and in vivo, termination is dependent on the integrity of this structure as well as the presence of a 3' polyuridine tra ...201424550474
mechanism for transfer of transposon tn2010 carrying macrolide resistance genes in streptococcus pneumoniae and its effects on genome evolution.the objective of this study was to identify the mechanism responsible for the horizontal transfer of transposon tn2010 in streptococcus pneumoniae, and the genomic alterations introduced by the transfer process.201424532683
antibacterial activity of synthetic curcumin derivatives: 3,5-bis(benzylidene)-4-piperidone (ef24) and ef24-dimer linked via diethylenetriaminepentacetic acid (ef2dtpa).curcumin is well known for its antimicrobial and anti-inflammatory properties. however, since systemic absorption and bioavailability of curcumin from gastrointestinal tract is considerably poor, synthetic curcuminoids are being developed as better alternatives. two curcumin derivatives: 3,5-bis(benzylidene)-4-piperidone (ef24) and ef24-dimer linked via diethylenetriaminepentacetic acid (ef2dtpa), were included in this study. we investigated the antibacterial activity of ef24 and ef2dtpa against ...201424532443
calcified iliopsoas abscess caused by enterococcus faecalis. 201424531098
comparative genomic and morphological analyses of listeria phages isolated from farm environments.the genus listeria is ubiquitous in the environment and includes the globally important food-borne pathogen listeria monocytogenes. while the genomic diversity of listeria has been well studied, considerably less is known about the genomic and morphological diversity of listeria bacteriophages. in this study, we sequenced and analyzed the genomes of 14 listeria phages isolated mostly from new york dairy farm environments as well as one related enterococcus faecalis phage to obtain information on ...201424837381
in vitro assessment of antimicrobial activity of pothomorphe umbellata extracts against enterococcus faecalis.due to the complex anatomy of the root canal system, biomechanical preparation is not able to completely eliminate all microorganisms present in the endodontic infections, making it necessary the use of an intracanal medication.201424748302
glycopeptide use is associated with increased mortality in enterococcus faecalis bacteraemia.enterococci are an important cause of nosocomial and community-acquired infections, with bacteraemia being one of the most common presentations. although inappropriate antimicrobial therapy has been associated with poorer outcomes in enterococcus faecalis (ef) bacteraemia, the impact of antimicrobial choice, namely β-lactam versus glycopeptide therapy, has not been well described. we sought to determine whether choice of antibiotic affects patient outcomes in ef bacteraemia.201424744303
passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against enterococcus faecalis.chemomechanical procedures can be used to eliminate bacteria from root canals. however, detectable bacteria sometimes remain because of the complexity of the root canal system. endodontic passive ultrasonic irrigation (pui) with hydrogen peroxide (h2o2) may be a promising option for increasing bactericidal hydroxyl radical (ho•) generation. in this in vitro experiment, we examined the effects of ho• generated using pui and a low concentration of h2o2. an ultrasonic tip was submerged in 0.45 mol/ ...201424739706
comparison of the antibacterial effect of sodium hypochlorite and aloe vera solutions as root canal irrigants in human extracted teeth contaminated with enterococcus faecalis.the main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. sodium hypochlorite has excellent antibacterial properties, but also some negative features.201424738089
an in vitro comparison of furcal perforation repaired with pro-root mta and new endodontic cement in primary molar teeth- a microleakage study.one of the most challenging procedural accidents during pulpotomy of primary molars is furcal perforation. to prevent bacterial invasion, the perforation site should be sealed as soon as possible.201424738087
occurrence of enterococcus species with virulence markers in an urban flow-influenced tropical recreational beach.median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<0.0001) with increasing proximity to river influx. the difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. virulence genes cyl, esp, gele and asa were detected at varying frequencies (7.32 ...201424725825
effects of resveratrol on gut microbiota and fat storage in a mouse model with high-fat-induced obesity.recent studies have investigated the anti-obesity effect of resveratrol, but the pathways through which resveratrol resists obesity are not clear. in the present study, we hypothesize that resveratrol exerts anti-obesity effects that are likely mediated by mechanisms of regulating gut microbes, and in turn, improving fat storage and metabolism. gut microbes, glucose and lipid metabolism in high-fat diet (hf) mice in vivo are investigated after resveratrol treatment. several biochemical markers a ...201424722352
structural elucidation of srta enzyme in enterococcus faecalis: an emphasis on screening of potential inhibitors against the biofilm formation.enterococcus faecalis is a pathogenic gram-positive bacterium, which mainly infects humans through urinary tract infections. srta is an essential enzyme for survival of e. faecalis, and inhibition of this particular enzyme will reduce the virulence of biofilm formation. it is proved to be associated with the microbial surface protein embedded signal transduction mechanism and promising as a suitable anti-microbial drug target for e. faecalis. the present work gives an inclusive description of sr ...201424718729
enhanced removal of enterococcus faecalis biofilms in the root canal using sodium hypochlorite plus photon-induced photoacoustic streaming: an in vitro study.the purpose of this study was to determine the effectiveness of laser-activated irrigation by photon-induced photoacoustic streaming (pips) using er:yag laser energy in decontaminating heavily colonized root canal systems in vitro.201424717113
impact of bacterial infections on aging and cancer: impairment of dna repair and mitochondrial function of host cells.the commensal floras that inhabit the gastrointestinal tract play critical roles in immune responses, energy metabolism, and even cancer prevention. pathogenic and out of place commensal bacteria, can however have detrimental effects on the host, by introducing genomic instability and mitochondrial dysfunction, which are hallmarks of both aging and cancer. helicobacter pylori and enterococcus faecalis are bacteria of the gastrointestinal tract that have been demonstrated to affect these two hall ...201424704713
colonization of enterococcus faecalis in a new sio/sio(2)-microtube in vitro model system as a function of tubule diameter.endodontic pathogens can penetrate deep into dentinal tubules and therefore survive the chemo-mechanical disinfection procedures. bacterial penetration has been mainly studies using sliced infected human teeth which, besides creating artifacts, can hinder the observation of the inner tubules due to the dense and opaque dentin structure. the aim of the present study was to develop a standardized dentin model by using artificial sio/sio2-microtubes of different diameters and lengths to test the pe ...201424703548
an in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against enterococcus faecalis.enterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic and acquired resistance to a variety of antibiotics. development of new antibiotics is limited, and pathogens continually generate new antibiotic resistance. many researchers aim to identify strategies to effectively kill this drug-resistant pathogen. here, we evaluated the effect of the antimicrobial peptide nisin on the antibacterial activities of 18 antibiotics against e. faecal ...201424586598
enterococcus infection biology: lessons from invertebrate host models.the enterococci are commensals of the gastrointestinal tract of many metazoans, from insects to humans. while they normally do not cause disease in the intestine, they can become pathogenic when they infect sites outside of the gut. recently, the enterococci have become important nosocomial pathogens, with the majority of human enterococcal infections caused by two species, enterococcus faecalis and enterococcus faecium. studies using invertebrate infection models have revealed insights into the ...201424585051
effects of single- and multi-strain probiotics on biofilm formation and in vitro adhesion to bladder cells by urinary tract pathogens.there is increasing evidence that probiotic bacteria can inhibit and/or prevent urinary tract infections. possible mechanisms include prevention of adhesion of pathogens to the bladder epithelium and inhibition of biofilm formation. currently there is interest in the comparative efficacy of single probiotics vs. strain mixtures. we have therefore tested the inhibitory activity of four single probiotics and four probiotic mixtures towards the urinary tract pathogens escherichia coli nctc 9001 and ...201424583095
photodynamic therapy versus ultrasonic irrigation: interaction with endodontic microbial biofilm, an ex vivo study.photodynamic therapy was introduced as an adjuvant to conventional chemo-mechanical debridement during endodontic treatment to overcome the persistence of biofilms. the aim of this study was to evaluate the ability of photodynamic therapy (pdt) to disrupt an experimental microbial biofilm inside the root canal in a clinically applicable working time.201424583092
recombinant human lipocalin 2 acts as an antibacterial agent to prevent platelet contamination.bacterial contamination of platelet products is the major infectious risk in blood transfusion medicine, which can result in life-threatening sepsis in recipient. lipocalin 2 (lcn2) is an iron-sequestering protein in the antibacterial innate immune response, which inhibit bacterial growth. this study was aimed to evaluate the antibacterial property of lcn2 in preventing bacterial contamination of platelets.201424580532
microwave-assisted preparation of azachalcones and their n-alkyl derivatives with antimicrobial activities.two new azachalcones were prepared by both claisen-schmidt condensation and a simple environmentally trendy microwave-assisted method. ten new n-alkyl (c6,8,10,12,14)-substituted azachalconium bromides (3a-e, 4a-e) were prepared from compounds 1 and 2 with corresponding alkyl halides. the antimicrobial activities of all the compounds were tested against enterococcus faecalis, yersinia pseudotuberculosis, pseudomonas aeruginosa, escherichia coli and staphylococcus aureus micro-organisms.201424571646
daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery.antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. in addition, daptomycin has been used recently against broad spectrum gram-positive organisms. the goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (pmma) bone cement and evaluate its feasibility for clinical use. daptomycin (0.5, 1, or 2 g) was premixed with 40 g ...201424571555
genome sequencing reveals the environmental origin of enterococci and potential biomarkers for water quality monitoring.enterococci are common members of the gut microbiome and their ease of culturing has facilitated worldwide use as indicators of fecal pollution of waters. however, enterococci were recently shown to persist in environmental habitats, often in the absence of fecal input, potentially confounding water quality assays. toward resolving this issue and providing a more complete picture of natural enterococci diversity, 11 isolates of enterococcus faecalis recovered from freshwater watersheds (environm ...201424571132
cell-recycle continuous fermentation of enterococcus faecalis rky1 for economical production of lactic acid by reduction of yeast extract supplementation.both lactic acid productivity and cell growth were linearly correlated with yeast extract supplementation in batch fermentation. during conventional continuous operation, although fresh feed was introduced into the bioreactor with a significantly low dilution rate (0.04 h(-1)), the amount of yeast extract employed was not enough to maintain the growth of microorganism. however, when the fresh feed contained 100 g/l glucose and 2 g/l yeast extract during cell-recycle continuous operation at a dil ...201424561722
systematic evaluation of commercial susceptibility testing methods for determining the in vitro activity of daptomycin versus staphylococcus aureus and enterococci.we systematically evaluated 5 methods for testing daptomycin versus 48 enterococcus faecalis, 51 enterococcus faecium, and 50 staphylococcus aureus isolates using (i and ii) broth microdilution (bmd) with 50-mg/liter calcium medium supplementation (reference method) and 30-mg/liter calcium medium supplementation (bmd30 method), (iii) etest, and (iv and v) microscan panel 33 using 2 methods to prepare the bacterial inoculum (microscan turbidity and microscan prompt). isolates were categorized as ...201424648560
daptomycin activity tested against 164457 bacterial isolates from hospitalised patients: summary of 8 years of a worldwide surveillance programme (2005-2012).we report the results of 8 years (2005-2012) of the daptomycin surveillance programme worldwide. consecutive non-duplicate bacterial isolates (prevalence design) were collected from patients with documented infections in 410 medical centres and were susceptibility tested by reference broth microdilution methods. a total of 164457 gram-positive isolates were evaluated, including 97542 staphylococcus aureus, 21413 coagulase-negative staphylococci (cons), 29619 enterococci and 15883 β-haemolytic st ...201424636430
green synthesis of silver nanoparticles using alternanthera dentata leaf extract at room temperature and their antimicrobial activity.a green rapid biogenic synthesis of silver nanoparticles agnps using alternanthera dentata (a. dentata) aqueous extract was demonstrated in this present study. the formation of silver nanoparticles was confirmed by surface plasmon resonance (spr) at 430nm using uv-visible spectrophotometer. the reduction of silver ions to silver nanoparticles by a. dentata extract was completed within 10min. synthesized nanoparticles were characterized using uv-visible spectroscopy; fourier transformed infrared ...201424632169
drug-resistant and hospital-associated enterococcus faecium from wastewater, riverine estuary and anthropogenically impacted marine catchment basin.enterococci, ubiquitous colonizers of humans and other animals, play an increasingly important role in health-care associated infections (hais). it is believed that the recent evolution of two clinically relevant species, enterococcus faecalis and enterococcus faecium occurred in a big part in a hospital environment, leading to formation of high-risk enterococcal clonal complexes (hireccs), which combine multidrug resistance with increased pathogenicity and epidemicity. the aim of this study was ...201424629030
antibacterial effect of iranian green-tea-containing mouthrinse vs chlorhexidine 0.2%: an in vitro study.considering the antioxidant, anti-inflammatory and antimicrobial properties of green tea, this study aimed to evaluate the antibacterial effect of mouthrinses containing green tea extract vs 0.2% chlorhexidine on selected microorganisms in vitro.201424624389
inactivation of airborne enterococcus faecalis and infectious bursal disease virus using a pilot-scale ultraviolet photocatalytic oxidation scrubber.high microbial concentrations and emissions associated with livestock houses raise health and environmental concerns. a pilot-scale ultraviolet photocatalytic oxidation (uv-pco) scrubber was tested for its efficacy to inactivate aerosolized enterococcus faecalis and infectious bursal disease virus (ibdv). microbial reduction was determined by the difference in microbial concentrations measured in the upstream and downstream isolators that were connected to the two ends of the uv-pco scrubber. tw ...201424620401
spread of multidrug-resistant enterococcus faecalis within the household setting.advances in veterinary medicine have resulted in the survival of many animals with severe illness or infectious diseases. in addition, increased usage of antimicrobial agents for veterinary purposes has contributed to the worldwide problem of increasing antimicrobial resistance. the objective of this study was to contribute to better understand the potential and implications for the spread of antimicrobial-resistant enterococci between pets receiving antimicrobial treatments and their owners. th ...201424617521
linezolid resistance in vancomycin-resistant enterococcus faecalis and enterococcus faecium isolates in a brazilian hospital. 201424614366
effects of intracanal irrigant mtad combined with nisin at sub-minimum inhibitory concentration levels on enterococcus faecalis growth and the expression of pathogenic genes.exposure to antibiotics is considered to be the major driver in the selection of antibiotic-resistant bacteria and may induce diverse biological responses in bacteria. mtad is a common intracanal irrigant, but its bactericidal activity remains to be improved. previous studies have indicated that the antimicrobial peptide nisin can significantly improve the bactericidal activity of mtad against enterococcus faecalis. however, the effects of mtad and its modification at sub-minimum inhibitory conc ...201424603760
antibacterial properties and mode of action of new triaryl butene citrate compounds.the aim of this study was to evaluate the antibacterial activity of newly synthesized triaryl butene analogues of tamoxifen. several compounds were synthesized and converted to citrate salts to ensure greater solubility. four compounds showed significant antibacterial activity at micromolar concentrations against gram-positive and gram-negative foodborne pathogens including listeria monocytogenes, listeria ivanovii, enterococcus faecalis, staphylococcus aureus and escherichia coli. two compounds ...201424602786
performance of the eucast disk diffusion method, the clsi agar screen method, and the vitek 2 automated antimicrobial susceptibility testing system for detection of clinical isolates of enterococci with low- and medium-level vanb-type vancomycin resistance: a multicenter study.different antimicrobial susceptibility testing methods to detect low-level vancomycin resistance in enterococci were evaluated in a scandinavian multicenter study (n=28). a phenotypically and genotypically well-characterized diverse collection of enterococcus faecalis (n=12) and enterococcus faecium (n=18) strains with and without nonsusceptibility to vancomycin was examined blindly in danish (n=5), norwegian (n=13), and swedish (n=10) laboratories using the eucast disk diffusion method (n=28) a ...201424599985
comparative evaluation of push-out bond strength of three endodontic sealers with and without amoxicillin-an invitro study.the purpose of this study was to evaluate the sealing ability of three different root canal sealers with and without amoxicillin, using a push-out bond strength test.201424596782
antibacterial substances from marine algae isolated from jeddah coast of red sea, saudi arabia.marine algae are known to produce a wide variety of bioactive secondary metabolites and several compounds have been derived from them for prospective development of novel drugs by the pharmaceutical industries. however algae of the red sea have not been adequately explored for their potential as a source of bioactive substances. in this context ulva reticulata, caulerpa occidentalis, cladophora socialis, dictyota ciliolata, and gracilaria dendroides isolated from red sea coastal waters of jeddah ...201424596500
etiological factors of infections in diabetic foot syndrome - attempt to define optimal empirical therapy.diabetic foot syndrome (dfs) represents one of the most frequent reasons for lower limb amputation in developed countries. in most cases, it is associated with bacterial infection, requiring optimal antibiotic therapy.201424596002
analysis of the promoters involved in enterocin as-48 expression.the enterocin as-48 is the best characterized antibacterial circular protein in prokaryotes. it is a hydrophobic and cationic bacteriocin, which is ribosomally synthesized by enterococcal cells and post-translationally cyclized by a head-to-tail peptide bond. the production of and immunity towards as-48 depend upon the coordinated expression of ten genes organized in two operons, as-48abc (where genes encoding enzymes with processing, secretion, and immunity functions are adjacent to the structu ...201424594763
the antibacterial activity, antioxidant activity and selectivity index of leaf extracts of thirteen south african tree species used in ethnoveterinary medicine to treat helminth infections.diseases caused by bacteria remain a major challenge globally and particularly in sub-saharan africa. the plants used in this study have been used in south africa to treat helminth infections in livestock and humans. in a previous study we found a correlation between antifungal and anthelmintic activity in some cases. in this study we examined other potential uses of these thirteen plant species by determining the antibacterial and antioxidant activity of the leaf acetone extracts.the antibacter ...201424589020
hospital acquired infections among patients admitted in the medical and surgical wards of a non-teaching secondary care hospital in northern india.to investigate the incidence of nosocomial infection (ni) and type of bacteriological isolates among the patients admitted in the medical and surgical wards of a non-teaching secondary care hospital in north india.201424701489
transcriptome analysis of enterococcus faecalis toward its adaption to surviving in the mouse intestinal tract.we have performed a transcriptomic in vivo study with enterococcus faecalis og1rf in the intestine of living mice to identify novel latent and adaptive fitness determinants within e. faecalis. from 2,658 genes that are present in e. faecalis strain og1rf, 124 genes were identified as significantly differentially expressed within the intestinal tract of living mice as compared to exponential growth in bhi broth. the groups of significantly up- or down-regulated genes consisted of 94 and 30 genes, ...201424700373
antimicrobial prenylated benzoylphloroglucinol derivatives and xanthones from the leaves of garcinia goudotiana.bioassay-guided fractionation using antimicrobial assay of the crude acetonic extract of garcinia goudotiana leaves and of its five partitions led to the isolation of two new prenylated benzoylphloroglucinol derivatives, goudotianone 1 (1) and goudotianone 2 (2), in addition to two known compounds including one xanthone, 1,3,7-trihydroxy-2-isoprenylxanthone (3), and one triterpenoid, friedelin (4). their structures were elucidated on the basis of different spectroscopic methods, including extens ...201424690454
the ability of triple antibiotic paste and calcium hydroxide in disinfection of dentinal tubules.the purpose of this in vitro study was to compare the ability of triple antibiotic paste (tap) to calcium hydroxide (ch) in disinfecting dentinal tubules.201424688581
antimicrobial effect of lippia sidoides and thymol on enterococcus faecalis biofilm of the bacterium isolated from root canals.the species lippia sidoides cham. (verbenaceae) is utilized in popular medicine as a local antiseptic on the skin and mucosal tissues. enterococcus faecalis is the bacterium isolated from root canals of teeth with persistent periapical lesions and has the ability to form biofilm, where it is responsible for the failure of endodontic treatments. essential oil of l. sidoides (eols) and its major component, thymol, were evaluated for reducing the cfu in biofilms of e. faecalis in vitro. the essenti ...201424683344
heterologous expression and characterization of tyrosine decarboxylase from enterococcus faecalis r612z1 and enterococcus faecium r615z1.tyrosine decarboxylase (tdc) is responsible for tyramine production and can catalyze phenylalanine to produce β-phenylethylamine. enterococcus strains are a group of bacteria predominantly producing tyramine and β-phenylethylamine in water-boiled salted duck. in this study, the heterologous expression and characterization of two tdcs from enterococcus faecalis r612z1 (612tdc) and enterococcus faecium r615z1 (615tdc) were studied. the recombinant putative proteins of 612tdc and 615tdc were hetero ...201424680070
influence of in vitro gastrointestinal digestion of fruit juices enriched with pine bark extract on intestinal microflora.the selective antimicrobial effect of fruit juices enriched with pine bark extract (pbe) (0.5 g/l) has been studied before and after in vitro gastrointestinal digestion. pbe (a concentrate of water-soluble bioflavonoids, mainly including phenolic compounds) has been proven to have high stability to the digestion process. pure phenolic compounds such as gallic acid had a high antimicrobial effect on staphylococcus aureus and escherichia coli, maintaining the lactic acid bacteria population (≈100% ...201424679746
identification of temporal clusters and risk factors of bacteremia by nosocomial vancomycin-resistant enterococci.this study aimed to evaluate a different methodology for addressing the evolution of nosocomial bacteremia by vancomycin-resistant enterococci (vre) in a hospital setting.201424679566
antimicrobial resistance and in vitro biofilm-forming ability of enterococci spp. isolated from urinary tract infection in a tertiary care hospital in dhaka.the biofilm mode of life conveys a survival advantage to the microorganism associated with it. biofilm on an indwelling urinary catheter consists of adherent microorganisms, their extra cellular products, and host components deposited on the catheter and thus biofilm on urinary catheters results in persistent infections that are resistant to antimicrobial therapy. this study was done during the period of january 2010 to december 2010. fifty nine enterococci isolated from 1203 urine samples were ...201426118165
in vitro comparison of the effects of probiotic, commensal and pathogenic strains on macrophage polarization.macrophages are important with respect to both innate and adaptive immune responses and are known to differentiate into pro-inflammatory m1- or anti-inflammatory m2-phenotypes following activation. in order to study how different bacteria affect macrophage polarization, we exposed murine raw 264.7 macrophages to sixteen different strains representing probiotic strains, pathogens, commensals and strains of food origin. increased inducible nitric oxide synthase (inos) or arginase-1 gene expression ...201424676762
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