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enterococcus faecalis activates caspase-1 leading to increased interleukin-1 beta secretion in macrophages.recent studies of inflammasome activation have focused on the pathogenesis of diverse inflammatory and autoimmune diseases. inflammasome activation results in caspase-1 activation, which is required for processing of prointerleukin (il)-1 beta to its secreted form as well as a proinflammatory cell death (ie, pyroptosis). the purpose of this study was to analyze whether enterococcus faecalis associated with endodontic infection induces inflammasome activation.201425127933
quantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone-water extracts of libidibia ferrea, parapiptadenia rigida and psidium guajava.vast numbers of plant species from northeastern brazil have not yet been phytochemically or biologically evaluated.201425124277
glycopeptide use is associated with increased mortality in enterococcus faecalis bacteraemia--authors' response. 201425118271
whole-animal chemical screen identifies colistin as a new immunomodulator that targets conserved pathways.the purpose of this study was to take advantage of the nematode caenorhabditis elegans to perform a whole-animal chemical screen to identify potential immune activators that may confer protection against bacterial infections. we identified 45 marketed drugs, out of 1,120 studied compounds, that are capable of activating a conserved p38/pmk-1 mitogen-activated protein kinase pathway required for innate immunity. one of these drugs, the last-resort antibiotic colistin, protected against infections ...201425118236
bacteriophages as an alternative strategy for fighting biofilm development.the ability of microbes to form biofilms is an important element of their pathogenicity, and biofilm formation is a serious challenge for today's medicine. fighting the clinical complications associated with biofilm formation is very difficult and linked to a high risk of failure, especially in a time of increasing bacterial resistance to antibiotics. bacterial species most commonly isolated from biofilms include coagulase-negative staphylococci, staphylococcus aureus, enterococcus faecalis, ent ...201425115107
antibiotic prophylaxis for transrectal prostate biopsy-a new strategy.fluoroquinolones are extensively used as prophylaxis for transrectal ultrasound-guided biopsy of the prostate (trubp). emerging fluoroquinolone resistance and selection of multiresistant organisms warrant new prophylactic strategies. pivmecillinam and amoxicillin/clavulanic acid have mutual synergistic activity and the combination of these agents has a broad coverage of the majority of microorganisms causing infectious complications after trubp and may be a valuable future prophylactic regimen.201425114164
platelet-rich plasma affects bacterial growth in vitro.platelet-rich plasma (prp), a blood derivative rich in platelets, is a relatively new technique used in tissue regeneration and engineering. the increased quantity of platelets makes this formulation of considerable value for their role in tissue healing and microbicidal activity. this activity was investigated against five of the most important strains involved in nosocomial infections (escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, klebsiella pneumoniae and streptococcus faec ...201425108654
prophages in enterococcal isolates from renal transplant recipients: renal failure etiologies promote selection of strains.infections caused by commensal bacteria may be fatal for the patients under immunosuppressive therapy. this results also from difficulty in identification of high risk strains. enterococcal infections are increasingly frequent but despite many studies on virulence traits, the difference between commensal and pathogenic strains remains unclear. prophages are newly described as important elements in competition between strains during colonization, as well as pathogenicity of the strains. here we e ...201425105126
draft genome sequence of linezolid-resistant enterococcus faecalis clinical isolate hs0914.we report the draft genome sequence of linezolid-resistant enterococcus faecalis strain hs-0914 isolated from a teaching hospital in shanghai, china. the draft genome sequence is composed of 61 contigs for 2,816,079 bp. ribosomal rna mutations and cfr, which mediates linezolid resistance, are not present.201425103766
influence of ultrasonic activation on photodynamic therapy over root canal system infected with enterococcus faecalis--an in vitro study.the purpose of this study was to evaluate, in vitro, the influence of ultrasonic activation on photodynamic therapy over root canal system infected with enterococcus faecalis.201425102161
development of a multiplex real-time pcr assay for the rapid diagnosis of neonatal late onset sepsis.the diagnosis of late onset sepsis (los), a severe condition with high prevalence in preterm infants, is hampered by the suboptimal sensitivity and long turnaround time of blood culture. detection of the infecting pathogen directly in blood by pcr would provide a much more timely result. unfortunately, pcr-based assays reported so far are labor intensive and often lack direct species identification. therefore we developed a real-time multiplex pcr assay tailored to los diagnosis which is easy-to ...201425102109
influence of first-line antibiotics on the antibacterial activities of acetone stem bark extract of acacia mearnsii de wild. against drug-resistant bacterial isolates.background. this study was aimed at evaluating the antibacterial activity of the acetone extract of a. mearnsii and its interactions with antibiotics against some resistant bacterial strains. methods. the antibacterial susceptibility testing was determined by agar diffusion and macrobroth dilution methods while the checkerboard method was used for the determination of synergy between the antibiotics and the extract. results. the results showed that the susceptibility of the different bacterial i ...201425101132
[neonatal bacteremia--frequency, spectrum of organisms and correlation between clinical symptoms and laboratory data for early onset sepsis].improved obstetrical management and evidence-based peripartum antimicrobial therapy in the last decades reduce the frequency of early-onset neonatal sepsis (eons) and improve the outcome. the spectrum of the microrganisms is different according to regions, countries and periods of time. appropriate diagnosis and treatment improve the outcome. however dissociation often occurs between clinical manifestation, laboratory and microbiological findings.201425098106
inspecting the potential physiological and biomedical value of 44 conserved uncharacterised proteins of streptococcus pneumoniae.the major gram-positive coccoid pathogens cause similar invasive diseases and show high rates of antimicrobial resistance. uncharacterised proteins shared by these organisms may be involved in virulence or be targets for antimicrobial therapy.201425096389
beneficial effects of heat-treated enterococcus faecalis fk-23 on high-fat diet-induced hepatic steatosis in mice.a high-fat diet (hfd) is one of the causes of hepatic steatosis. we previously demonstrated that enterococcus faecalis fk-23 (fk-23), a type of lactic acid bacteria, exhibits an anti-obesity effect in mice fed a hfd. in the present study, we examined the effects of fk-23 on hfd-induced hepatic steatosis. male c57bl/6 mice were divided into four groups and given one of four treatments: standard diet (sd); standard diet supplemented with fk-23 (sd+fk); hfd; or hfd supplemented with fk-23 (hfd+fk). ...201425089585
endophthalmitis caused by enterococcus faecalis: clinical features, antibiotic sensitivities, and outcomes.to report the clinical features, antibiotic sensitivities, and visual acuity outcomes of endophthalmitis caused by enterococcus faecalis.201425089354
antibacterial peptide nisin: a potential role in the inhibition of oral pathogenic bacteria.although the antimicrobial peptide nisin has been extensively studied in the food industry for decades, its application in the oral cavity remains to develop and evaluate its feasibility in treating oral common diseases. nisin is an odorless, colorless, tasteless substance with low toxicity and with antibacterial activities against gram-positive bacteria. these biologic properties may establish its use in promising products for oral diseases. this article summarizes the antibacterial efficiency ...201425088158
pyranoside-into-furanoside rearrangement: new reaction in carbohydrate chemistry and its application in oligosaccharide synthesis.great interest in natural furanoside-containing compounds has challenged the development of preparative methods for their synthesis. herein a novel reaction in carbohydrate chemistry, namely a pyranoside-into-furanoside (pif) rearrangement permitting the transformation of selectively o-substituted pyranosides into the corresponding furanosides is reported. the discovered process includes acid-promoted sulfation accompanied by rearrangement of the pyranoside ring into a furanoside ring followed b ...201425319316
optimizing therapy for vancomycin-resistant enterococcal bacteremia in children.uncertainties exist regarding the optimal treatment for vancomycin-resistant enterococcal (vre) bloodstream infections, particularly in settings in which ampicillin cannot be used.201425313503
role of efrab efflux pump in biocide tolerance and antibiotic resistance of enterococcus faecalis and enterococcus faecium isolated from traditional fermented foods and the effect of edta as efrab inhibitor.enterococcus faecalis and enterococcus faecium isolated from various traditional fermented foods of both animal and vegetable origins have shown multidrug resistance to several antibiotics and tolerance to biocides. reduced susceptibility was intra and inter-species dependent and was due to specific and unspecific mechanisms such as efflux pumps. efrab, a heterodimeric abc transporter efflux pump, was detected in 100% of multidrug resistant (mdr) e. faecalis strains and only in 12% of mdr e. fae ...201425084670
disinfection efficacy of photon-induced photoacoustic streaming on root canals infected with enterococcus faecalis: an ex vivo study.in 2010, one of the authors proposed that lasers could be used to enhance the decontaminating action of sodium hypochlorite (naocl). the authors conducted a study to compare the disinfection efficacy of laser-activated irrigation (lai) by using a photon-induced photoacoustic streaming (pips) tip with conventional irrigation and specifically lai's ability to remove bacterial film formed on root canal walls.201425082933
identification of polyketide inhibitors targeting 3-dehydroquinate dehydratase in the shikimate pathway of enterococcus faecalis.due to the emergence of resistance toward current antibiotics, there is a pressing need to develop the next generation of antibiotics as therapeutics against infectious and opportunistic diseases of microbial origins. the shikimate pathway is exclusive to microbes, plants and fungi, and hence is an attractive and logical target for development of antimicrobial therapeutics. the gram-positive commensal microbe, enterococcus faecalis, is a major human pathogen associated with nosocomial infections ...201425072253
antibiofilm effects of endodontic sealers containing quaternary ammonium polyethylenimine nanoparticles.this study evaluated the antibiofilm effects of 2 endodontic sealers incorporated with quaternary ammonium polyethylenimine (qpei) nanoparticles at a 2% concentration (w/w).201425069926
effective analysis of the use of peracetic acid after instrumentation of root canals contaminated with enterococcus faecalis.the aim of this study was to evaluate the effectiveness of peracetic acid (paa) in cleaning root canals contaminated with enterococcus faecalis.201425069922
antimicrobial effects of four intracanal medicaments on enterococcus faecalis: an in vitro study.the aim of this in vitro study was to evaluate the antimicrobial activity of four intracanal medicaments on enterococcus faecalis (e. faecalis).201425031593
a microleakage study of gutta-percha/ah plus and resilon/real self-etch systems after different irrigation protocols.the development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. the resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin.201425025557
using color histograms and spa-lda to classify bacteria.in this work, a new approach is proposed to verify the differentiating characteristics of five bacteria (escherichia coli, enterococcus faecalis, streptococcus salivarius, streptococcus oralis, and staphylococcus aureus) by using digital images obtained with a simple webcam and variable selection by the successive projections algorithm associated with linear discriminant analysis (spa-lda). in this sense, color histograms in the red-green-blue (rgb), hue-saturation-value (hsv), and grayscale cha ...201425023972
revised reference broth microdilution method for testing telavancin: effect on mic results and correlation with other testing methodologies.the reference broth microdilution (bmd) antimicrobial susceptibility testing method for telavancin was revised to include dimethyl sulfoxide (dmso) as a solvent and diluent for frozen-form panel preparation, following the clsi recommendations for water-insoluble agents. polysorbate 80 (p-80) was also added to the test medium to minimize proven drug losses associated with binding to plastic surfaces. four hundred sixty-two gram-positive isolates, including a challenge set of organisms with reduce ...201425022579
role of the bacterial vaccine solco-urovac® in treatment and prevention of recurrent urinary tract infections of bacterial origin.urinary tract infections (uti) represent a serious medical problem with considerably high rate of morbidity. recurrent episodes of urinary tract infection (ruti) may commonly develop in the way of relapse or reinfection. for patients, ruti is always associated with costs, discomfort and decreased quality of life. standard treatment of ruti is through antibiotics and usually such treatment is required repeatedly. repeat course of antibiotics leads to increase of resistance of uropathogenic strain ...201425020163
susceptibility of methacrylate-based root canal filling to degradation by bacteria found in endodontic infections.to present a case of endodontic failure obturated with a methacrylate-based root filling material, resilon/realseal (rs). to determine if rs is susceptible to biodegradation by endodontically relevant microbes by a method known to show rs degradation.201425019109
erythromycin- and copper-resistant enterococcus hirae from marine sediment and co-transfer of erm(b) and tcrb to human enterococcus faecalis.an erythromycin-, copper- and cadmium-resistant isolate of enterococcus hirae from marine sediment was shown to harbor the plasmid pre25 and to co-transfer erm(b) and tcrb to enterococcus faecalis jh2-2. these data highlight the scope for antibiotic resistance selection by the marine environment through heavy metals and its possible involvement in antibiotic-resistant enterococcal infections.201425017384
bacterial entombment by intratubular mineralization following orthograde mineral trioxide aggregate obturation: a scanning electron microscopy study.the time domain entombment of bacteria by intratubular mineralization following orthograde canal obturation with mineral trioxide aggregate (mta) was studied by scanning electron microscopy (sem). single-rooted human premolars (n=60) were instrumented to an apical size #50/0.06 using profile and treated as follows: group 1 (n=10) was filled with phosphate buffered saline (pbs); group 2 (n=10) was incubated with enterococcus faecalis for 3 weeks, and then filled with pbs; group 3 (n=20) was obtur ...201425012869
comment on: glycopeptide use is associated with increased mortality in enterococcus faecalis bacteraemia. 201425011652
[antibiotic resistance and siderophore production in enterococci].enterococci belong to the normal bacterial flora of the gastrointensinal tract of humans. enterococci are regarded as harmless commensal, and are even believed to have probiotic characteristics. however, they can cause variety of infections, including endocarditis, bloodstream infections and urinary tract infections. during the past several decades, enterococci, and particularly enterococcus faecalis and e. faecium, have been identified as an important cause of nosocomial infections. enterococci ...201425007507
antibacterial activity of various root canal sealers and root-end filling materials in dentin blocks infected ex vivo with enterococcus faecalis.the aim was to investigate the antibacterial activity of the root-end filling materials mta and irm, different endodontic sealers and calcium hydroxide [ca(oh)₂] in experimentally infected dentinal tubules.201425005627
a in-vitro comparative study on bacterial leakage of mineral trioxide aggregate, calcium enriched cement and bone cement in furcal perforations.the aim of this study was to compare the bacterial leakage of mineral trioxide aggregate (mta), calciem enriched cement (cem), and bone cement (bc) as repair materials in furcal perforations.201425001012
single consumption of bryndza cheese temporarily affects oral microbiota and salivary markers of oxidative stress.several oral diseases are associated with changes in oral microbiota and higher oxidative stress. enterococcus faecalis has been hypothesized to directly contribute to the oxidative stress in oral cavity. the aim of this study was to examine the effect of single consumption of unpasteurized bryndza cheese containing enterococci on changes of microbiota and oxidative status in saliva. fourteen healthy volunteers aged 23-30 years were asked to eat 100 g of bryndza cheese. saliva samples were colle ...201424997802
gestational age-specific reference ranges of hepcidin in cord blood.iron deficiency (id) contributes to anaemia of prematurity, and hence the reliable assessment of iron nutrition status appears to be mandatory.201424993575
stability of antibacterial activity of chlorhexidine and doxycycline in bovine root dentine.the aim of this study was to compare the substantivity of chlorhexidine (chx) and doxycycline bond to the dentin in diffusion disk method.201424991631
efficacy of coral-hydroxyapatite and biphasic calcium phosphate for early bacterial detection.nano- or microhydroxyapatites with microbiological properties are being used to detect pathogens in clinical samples and industrial environments. in this study, the calcium phosphates coral-hydroxyapatite and biphasic calcium phosphate were characterized physicochemically using x-ray diffraction, thermogravimetric, and differential thermal analysis. the morphology, texture, and chemical composition of the ceramics were also investigated using scanning electron microscopy with energy dispersive s ...201424985222
mechanisms of vancomycin resistance in staphylococcus aureus.vancomycin is a glycopeptide antibiotic used for the treatment of gram-positive bacterial infections. traditionally, it has been used as a drug of last resort; however, clinical isolates of methicillin-resistant staphylococcus aureus (mrsa) strains with decreased susceptibility to vancomycin (vancomycin intermediate-resistant s. aureus [visa]) and more recently with high-level vancomycin resistance (vancomycin-resistant s. aureus [vrsa]) have been described in the clinical literature. the rare v ...201424983424
molecular basis for bacterial peptidoglycan recognition by lysm domains.carbohydrate recognition is essential for growth, cell adhesion and signalling in all living organisms. a highly conserved carbohydrate binding module, lysm, is found in proteins from viruses, bacteria, fungi, plants and mammals. lysm modules recognize polysaccharides containing n-acetylglucosamine (glcnac) residues including peptidoglycan, an essential component of the bacterial cell wall. however, the molecular mechanism underpinning lysm-peptidoglycan interactions remains unclear. here we des ...201424978025
application of multilocus sequence analysis for molecular characterization of enterococci with virulence factors recovered from a tropical recreational beach.partial gene sequences of phenylalanyl-trna synthase alpha subunit (phes) and rna polymerase alpha subunit (rpoa) were evaluated for species delineation and detection of recombination among enterococci populations recovered from a bathing beach impacted by low tide river flow. at inter-species level, a maximum similarity of 86.5% and 94.8% was observed among the enterococci phes and rpoa sequence, respectively. a superimposed plot of delimited pairwise similarity values obtained for 266 pair-wis ...201424974655
[in vitro antimicrobial susceptibility in enterococcus faecalis and enterococcus faecium isolated from hospitalized patients].enterococcus spp is currently considered as one of the most important nosocomial pathogens . the antibiotic resistance of this group of bacteria is a particularly important problem in health centers.201424968036
the antimicrobial activity of azadirachta indica, mimusops elengi, tinospora cardifolia, ocimum sanctum and 2% chlorhexidine gluconate on common endodontic pathogens: an in vitro study.to check the antimicrobial activity of azadirachta indica (neem), ocimum sanctum (tulsi), mimusops elelngi (bakul), tinospora cardifolia (giloy) and chlorhexidine gluconate (chx) on common endodontic pathogens like streptococcus mutans, enterococcus faecalis and staphylococcus aureus.201424966766
effect of "apical clearing" and "apical foramen widening" on apical ramifications and bacterial load in root canals.the purpose of the study was to determine the effect of apical clearing and apical foramen widening in reducing apical ramifications and bacterial load in the apical third of root canals. the mesio-buccal roots of 21 maxillary first molar teeth were inoculated with enterococcus faecalis suspension using a sterile pipette. samples were incubated at 37°c for 72 hrs and divided into 3 groups: group a, control group (n=5), no preparation; group b (n=8) conventional preparation alone; and group c (n= ...201424965951
prevalence of asymptomatic bacteriuria and its antibacterial susceptibility pattern among pregnant women attending the antenatal clinic at kanpur, india.symptomatic and asymptomatic bacteriuria (asb) is common in pregnant women. pregnancy enhances the progression from asb to symptomatic bacteriuria, which if left untreated, could lead to acute pyelonephritis and other adverse outcomes such as prematurity, postpartum, hypertensive disease, anaemia, utis and higher foetal mortality rates.201424959438
defining daptomycin resistance prevention exposures in vancomycin-resistant enterococcus faecium and e. faecalis.daptomycin is used off-label for enterococcal infections; however, dosing targets for resistance prevention remain undefined. doses of 4 to 6 mg/kg of body weight/day approved for staphylococci are likely inadequate against enterococci due to reduced susceptibility. we modeled daptomycin regimens in vitro to determine the minimum exposure to prevent daptomycin resistance (dapr) in enterococci. daptomycin simulations of 4 to 12 mg/kg/day (maximum concentration of drug in serum [cmax] of 57.8, 93. ...201424957825
[epidemiological analysis for enterococci isolated in aichi prefecture].we investigated vancomycin-resistant genes for clinical isolates of 353 vancomycin-resistant enterococci in the aichi medical university hospital and 120 vancomycin-resistant enterococci from the 8 facilities in aichi prefecture between april 2008 and january 2013. we detected 8, 105, 21 and 4 strains of enterococci with vana, vanb, vanc1 and vanc2/c3, respectively. among enterococci with vancomycin-resistant genes, we detected 4, 3 and 2 enterococci of vancomycin mic level 4 microg/ml with vanb ...201424956911
blood stream infections in renal transplant recipients: a single-center study.bacteremias among renal transplant recipients are more frequent as a result of immunosuppression. they are considered extremely high-risk because they are correlated with decreased allograft and recipient survival.201425420856
mechanisms of linezolid resistance in staphylococci and enterococci isolated from two teaching hospitals in shanghai, china.linezolid is one of the most effective treatments against gram-positive pathogens. however, linezolid-resistant/intermediate strains have recently emerged in worldwide. the purpose of this study was to analyse the prevalence and resistance mechanisms of linezolid-resistant/intermediate staphylococci and enterococci in shanghai, china.201425420718
effects of adsorbed and templated nanosilver in mesoporous calcium-silicate nanoparticles on inhibition of bacteria colonization of dentin.mesoporous calcium-silicate nanoparticles (mcsns) are advanced biomaterials for controlled drug delivery and mineralization induction. nanosilver-incorporated mcsns (ag-mcsns) were prepared in the present study using both the adsorption and template methods. both versions of ag-mcsns showed characteristic morphology of mesoporous materials and exhibited sustained release of ions over time. in antibacterial testing against planktonic enterococcus faecalis, ag-mcsns showed significantly better ant ...201425419127
molecular characterization and antibiotic resistance of enterococcus species from gut microbiota of chilean altiplano camelids.enterococcus is one of the major human pathogens able to acquire multiple antibiotic-resistant markers as well as virulence factors which also colonize remote ecosystems, including wild animals. in this work, we characterized the enterococcus population colonizing the gut of chilean altiplano camelids without foreign human contact.201425405007
bacterial leakage in root canals filled with resin-based and mineral trioxide aggregate-based sealers.sealing ability is one of the most important features of endodontic sealers. the purpose of this in vitro study was to compare the sealing ability of a resin-based sealer with a mineral trioxide aggregate-based sealer.201425426153
comparative structural and functional analysis of staphylococcus aureus glucokinase with other bacterial glucokinases.glucokinase is classified in bacteria based upon having atp binding site and 'repressor/open reading frames of unknown function/sugar kinases' motif, the sequence of glucokinase gene (jn645812) of staphylococcus aureus atcc12600 showed presence of atp binding site and 'repressor/open reading frames of unknown function/sugar kinases' motif. we have earlier observed glucokinase of s. aureus has higher affinity towards the substrate compared to other bacterial glucokinase and under anaerobic condit ...201425425757
anti-bacterial and anti-oxidant activities of leaf extracts of combretum vendae (combretecacea) and the isolation of an anti-bacterial compound.combretum vendae a.e. van wyk (combretaceae) is used for the treatment of bacterial related infections and oxidative related diseases by indigenous people of south africa. dried leaves extracts of c. vendae were investigated for bioactivity against a variety of bacterial strains and their antioxidant potential evaluated.201425395708
australian enterococcal sepsis outcome progamme, 2011.from 1 january to 31 december 2011, 29 institutions around australia participated in the australian enterococcal sepsis outcome programme (aesop). the aim of aesop 2011 was to determine the proportion of enterococcal bacteraemia isolates in australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterise the molecular epidemiology of the enterococcus faecalis and e. faecium isolates. of the 1,079 unique episodes of ba ...201425391408
comparative prevalence of antimicrobial resistance in community-acquired urinary tract infection cases from representative states of northern and southern india.urinary tract infections (utis) are amongst the most common infections described in outpatient settings. increased antimicrobial resistance (amr) of urinary tract pathogens is a matter of global public health concern. treatment of uti depends on both prevalence and antimicrobial resistance (amr) of causative bacteria at any specific geographical location.201425386432
an in vitro comparison of the antibacterial efficacy of triphala with different concentrations of sodium hypochlorite.the antimicrobial efficacy of root canal irrigant plays an important role in increasing the success of root canal treatment (rct). the aim of the present experimental study was to compare the antimicrobial activity of triphala (a plant-derived solution) with 0.5, 1, 2.5 and 5% concentrations of sodium hypochlorite (naocl), against enterococcus faecalis (e. faecalis).201425386211
effect of photodynamic therapy (pdt) on enterococcus faecalis biofilm in experimental primary and secondary endodontic infections.to determine the antibacterial effect of photodynamic therapy on enterococcus faecalis (e. faecalis) biofilms in experimentally infected human root canals in primary infections and endodontic retreatments.201425366394
comparison of the antibacterial efficacy of manuka honey against e.faecalis and e.coli - an in vitro study.to compare the antibacterial efficacy of manuka honey against e.faecalis and e.coli.201425302263
a delicate balance: maintaining mutualism to prevent disease.the intestinal microbial ecosystem is complex, and few of the principles that contribute to homeostasis in health are well understood. pham et al. (2014) show that a network including the epithelial interleukin-22 receptor protects against infection with the opportunistic pathogen enterococcus faecalis through promotion of host-microbiota mutualism.201425299326
viability and antibacterial efficacy of four root canal disinfection techniques evaluated using confocal laser scanning microscopy.several disinfection techniques have been recently introduced with the main objective of improving root canal disinfection in the inaccessible areas of the root canal system. this in vitro study was done to evaluate the antimicrobial effect and viability of enterococcus faecalis biofilms using conventional irrigation, endoactivator (dentsply, tulsa dental, usa), diode laser irradiation and photon-initiated photoacoustic streaming (pips).201425298645
antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against enterococcus faecalis biofilm.the aim of the present study was to compare the antibacterial effect of calcium hydroxide (ch), triantibiotic mixture (tam), and ch in combination with chlorhexidine (chx), sodium hypochlorite (naocl) or colchicine (col) against enterococcus faecalis (e. faecalis) in surface and deep dentinal tubules. seventy five fresh single-rooted human teeth were infected and divided into five experimental groups (n=15). the experimental groups were treated with ch+distilled water, ch+chx, ch+naocl, ch+col+d ...201425297855
a novel role for d-alanylation of lipoteichoic acid of enterococcus faecalis in urinary tract infection.enterococci are the third most common cause of healthcare-associated infections, which include urinary tract infections, bacteremia and endocarditis. cell-surface structures such as lipoteichoic acid (lta) have been poorly examined in e. faecalis, especially with respect to urinary tract infections (utis). the dlt operon is responsible for the d-alanylation of lta and includes the gene dlta, which encodes the d-alanyl carrier protein ligase (dcl). the involvement of lta in uti infection by e. fa ...201425296179
in vitro activity of oritavancin against gram-positive pathogens isolated in canadian hospital laboratories from 2011 to 2013.gram-positive pathogens isolated in 15 canadian hospital laboratories between 2011 and 2013 were tested for susceptibility to oritavancin and comparative antimicrobial agents using the clinical and laboratory standards institute broth microdilution method. oritavancin demonstrated in vitro activity equivalent to, or more potent than, vancomycin, daptomycin, linezolid, and tigecycline against the isolates of methicillin-susceptible staphylococcus aureus (n=1460; oritavancin mic90, 0.06 μg/ml; 99. ...201425294303
large vegetation in a 60-year-old man with enterococcus faecalis cardiac implantable electronic device infection. 201425293687
complete genome assembly of enterococcus faecalis 29212, a laboratory reference strain.enterococcus faecalis is a nonmotile gram-positive coccus, found both as a commensal organism in healthy humans and animals and as a causative agent of multiple diseases, in particular endocarditis. we sequenced the genome of e. faecalis atcc 29212, a commonly used reference strain in laboratory studies, to complete "finished" annotated assembly (3 mb).201425291775
infantile subdural empyema: the role of brain sonography and percutaneous subdural tapping in a resource-challenged region.this study explored the outcome of children with patent anterior fontanelles who were treated with trans-fontanelle ultrasound scan (tfuss), which is more affordable and available than ct scan and mri in the diagnosis of childhood intracranial pathologies and treatment of subdural empyema, in developing countries.201425288836
effects of storage temperature on tyramine production by enterococcus faecalis r612z1 in water-boiled salted ducks.tyramine production by enterococcus faecalis r612z1 in water-boiled salted ducks was evaluated during storage at different temperatures. the results showed that e. faecalis r612z1 could produce tyramine in meat samples when the storage temperature was no less than 4°c. the e. faecalis r612z1 counts of the meat samples reached 10(8) cfu/g on day 7 at 4°c and on day 4 at 10°c. however, the tyramine content of the meat samples stored at 10°c increased to 23.73 μg/g (on day 10), which was greater th ...201425285502
mapping of the modular closthioamide architecture reveals crucial motifs of polythioamide antibiotics.closthioamide, the first known secondary metabolite from an anaerobic microorganism (clostridium cellulolyticum), represents a highly potent antibiotic that is active against methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus faecalis (vre) at nanomolar concentrations. to unveil structure-activity relationships of the unusual polythioamide natural product we have designed a synthetic grid to access analogues with altered terminal aromatic moieties, diverse p ...201425284750
a comparative evaluation of antibacterial efficacy of 'activ points' and 'combi points' as intra-canal medicaments against enterococcus faecalis: an ex vivo study.enterococcus faecalis has been found to be one of the most predominant bacterial species associated with failed endodontic cases. the ability of this microbe to form biofilms; penetrate into dentinal tubules; survival in low ph, high salinity and high temperatures and resistance to many intracanal medicaments, makes it one of the most resistant pathogen of all the root canal flora. studies have shown that chlorhexidine is relatively more effective against e. faecalis as compared to other intra c ...201425284521
new antibacterial xanthone from the marine sponge-derived micrococcus sp. eg45.microluside a [4 (19-para-hydroxy benzoyloxy-o-β-d-cellobiosyl), 5 (30-para-hydroxy benzoyloxy-o-β-d-glucopyranosyl) xanthone (1)] is a unique o-glycosylated disubstituted xanthone isolated from the broth culture of micrococcus sp. eg45 cultivated from the red sea sponge spheciospongia vagabunda. the structure of microluside a was determined by 1d- and 2d-nmr techniques as well as high resolution tandem mass spectrometry. the antimicrobial activity evaluation showed that 1 exhibited antibacteria ...201425283555
comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis: an in vitro study.the purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (ca[ocl]2) and sodium hypochlorite (naocl) associated with passive ultrasonic irrigation in root canals of bovine teeth infected with enterococcus faecalis.201425282371
biohybrid polymer-antimicrobial peptide medium against enterococcus faecalis.antimicrobial peptides (amps) are conserved evolutionary components of the innate immune system that are being tested as alternatives to antibiotics. slow release of amps using biodegradable polymers can be advantageous in maintaining high peptide levels for topical treatment, especially in the oral environment in which dosage retention is challenged by drug dilution with saliva flow and by drug inactivation by salivary enzymatic activity. enterococcus faecalis is a multidrug resistant nosocomia ...201425279943
evolution of the resistance to antibiotics of bacteria involved in urinary tract infections: a 7-year surveillance study.we conducted a retrospective analysis on the identification and antibiogram of all bacteria isolated from urine samples with microbiological confirmation of urinary tract infection (uti) in a spanish reference hospital over a 7-year period.201425278389
commensal bacteria directly suppress in vitro degranulation of mast cells in a myd88-independent manner.the intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. however, the cellular and molecular mechanisms underlying these effects remain to be determined. here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a myd88-independent manner. i ...201425273132
identification of peptidoglycan-associated proteins as vaccine candidates for enterococcal infections.infections by opportunistic bacteria have significant contributions to morbidity and mortality of hospitalized patients and also lead to high expenses in healthcare. in this setting, one of the major clinical problems is caused by gram-positive bacteria such as enterococci and staphylococci. in this study we extract, purify, identify and characterize immunogenic surface-exposed proteins present in the vancomycin resistant enterococci (vre) strain enterococcus faecium e155 using three different e ...201425369230
antibacterial efficacy of aqueous ozone in root canals infected by enterococcus faecalis.in endodontics, the elimination of resistant bacteria such as enterococcus faecalis plays an important role for treatment success in root canals. therefore, new alternative irrigants (instead of sodium hypochlorite) have been researched to achieve ideal endodontic treatment.201425368798
healthcare-associated infective endocarditis: a case series in a referral hospital from 2006 to 2011.healthcare-associated infective endocarditis (hca-ie), a severe complication of medical care, shows a growing incidence in literature.201425352503
[study of association between parvimonas micra and pulp dominant pathogens in the infected root canals with chronic periradicular periodontitis].to study the prevalence of parvimonas micra (pm) and the associations between pm and pulp dominant pathogens in order to reflect the colonization of pm in the infected root canals with chronic periradicular periodontitis.201425351224
riboflavin-shuttled extracellular electron transfer from enterococcus faecalis to electrodes in microbial fuel cells.great attention has been focused on gram-negative bacteria in the application of microbial fuel cells. in this study, the gram-positive bacterium enterococcus faecalis was employed in microbial fuel cells. bacterial biofilms formed by e. faecalis zer6 were investigated with respect to electricity production through the riboflavin-shuttled extracellular electron transfer. trace riboflavin was shown to be essential for transferring electrons derived from the oxidation of glucose outside the peptid ...201425345758
engineering acetyl coenzyme a supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of saccharomyces cerevisiae.the energetic (atp) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. cytosolic acetyl coenzyme a (acetyl-coa) is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into saccharomyces cerevisiae, such as isoprenoids or lipids. in this yeast, synthesis of cytosolic acetyl-coa via acetyl-coa synthetase (acs) involves hydrolysis of atp to amp and pyrophosphat ...201425336454
central line associated blood stream infection rate after intervention and comparing outcome with national healthcare safety network and international nosocomial infection control consortium data.benchmarking of central line associated blood stream infection (clabsi) rates remains a problem in developing countries due to the variations in surveillance practices and/or infection risk as non-availability of national data.201425328774
selection of potential probiotic enterococcus faecium isolated from portuguese fermented food.four enterococcus faecium strains isolated from fermented products were evaluated for potential use as probiotic strains. in addition to efaafm gene, commonly found in e. faecium food isolates, none of the isolates possessed virulence genes and none had positive reactions for the production of tyramine, histamine, putrescine and cadaverine in the screening medium used. all of these four isolates proved to be resistant to 65 °c. e. faecium 119 did not show antimicrobial activity against any of th ...201425268323
a comparative study on sealing ability of mineral trioxide aggregate, calcium enriched cement and bone cement in furcal perforations.the aim of this study was to compare the bacterial leakage of mineral trioxide aggregate (mta), calcium enriched cement (cem), and bone cement (bc) as repair materials in furcal perforations.201425267149
antimicrobial activity of a sodium hypochlorite/etidronic acid irrigant solution.the aim of this study was to evaluate the antimicrobial activity of a 2.5% sodium hypochlorite (naocl)/9% etidronic acid (hebp) irrigant solution on enterococcus faecalis growing in biofilms and a dentinal tubule infection model.201425266466
in vitro evaluation of antibacterial effect of ah plus incorporated with quaternary ammonium epoxy silicate against enterococcus faecalis.the purpose of this study was to evaluate the in vitro antibacterial effect of ah plus (dentsply, detrey, konstanz, germany) incorporated with quaternary ammonium epoxy silicate (qaes) against enterococcus faecalis.201425260732
a widely used in vitro biofilm assay has questionable clinical significance for enterococcal endocarditis.biofilm formation may play an important role in the pathogenesis of infections caused by enterococcus faecalis, including endocarditis. most biofilm studies use a polystyrene dish assay to quantify biofilm biomass. however, recent studies of e. faecalis strains in tissue and animal models suggest that polystyrene dish results need to be interpreted with caution. we evaluated 158 clinical e. faecalis isolates using a polystyrene dish assay and found variation in biofilm formation, with many isola ...201425255085
intestinal translocation of clinical isolates of vancomycin-resistant enterococcus faecalis and esbl-producing escherichia coli in a rat model of bacterial colonization and liver ischemia/reperfusion injury.the objectives of this study were to develop a rat model of gastrointestinal colonization with vancomycin-resistant enterococcus faecalis (vre) and extended-spectrum beta-lactamase (esbl)-producing e. coli and to evaluate intestinal translocation to blood and tissues after total and partial hepatic ischemia. methods - we developed a model of rat colonization with vre and esbl-e coli. then we studied four groups of colonized rats: group i (with hepatic pedicle occlusion causing complete liver isc ...201425255079
measurement of cytokine levels in cerebrospinal fluid over time in neonatal enterococcal meningitis.enterococcus faecalis is rarely involved in neonatal meningitis. several studies have indicated that the cytokines related to bacterial infection may induce nerve cell damage; therefore, the cytokine levels in cerebrospinal fluid (csf) could represent a valuable hallmark for rapid recognition of the disease and evaluation of the degree of neurological involvement. we analyzed cytokine levels in the csf of a neonate with e. faecalis meningitis over time. tumor necrosis factor-α (tnf-α) tended to ...201425252071
antimicrobial efficacy of er,cr:ysgg laser-activated irrigation compared with passive ultrasonic irrigation and rinsendo(®) against intracanal enterococcus faecalis.the aim of this study was to evaluate the antibacterial efficacy of active irrigation techniques: erbium, chromium: yttrium, scandium, gallium, garnet (er,cr:ysgg) laser-activated irrigation (lai), passive ultrasonic irrigation (pui), rinsendo(®), and conventional syringe irrigation, against intracanal enterococcus faecalis.201425251217
antibacterial power of sodium hypochlorite combined with surfactants and acetic acid.the aim of this ex vivo study was to compare the antibacterial power of 1% naocl with 1% acetic acid, 5.25% naocl and two commercially available naocl modified with surfactants in bovine root dentin. a total of 120 dentin tubes prepared from intact bovine incisors were infected for 21 days with enterococcus faecalis and randomly divided into six groups as follows: 5.25%naocl; hypoclean; chlor-xtra; 1% naocl with 1% acetic acid; infected dentin tubes (positive control); and sterile dentin tubes ( ...201425250491
peritonitis in a continuous ambulatory peritoneal dialysis patient by two different species of enterococci: a rare finding.peritonitis in a continuous ambulatory peritoneal dialysis patient by two different species of enterococci is a rare condition. we report a case of peritonitis from which vancomycin sensitive enterococcus faecalis and vancomycin resistant enterococcusc faecium were isolated. it also emphasizes the effectiveness of linezolid for the treatment of vancomycin resistant enterococcal infection.201425249726
an abc transporter is required for secretion of peptide sex pheromones in enterococcus faecalis.enterococci are leading causes of hospital-acquired infection in the united states and continue to develop resistances to new antibiotics. many enterococcus faecalis isolates harbor pheromone-responsive plasmids that mediate horizontal transfer of even large blocks of chromosomal genes, resulting in hospital-adapted strains over a quarter of whose genomes consist of mobile elements. pheromones to which the donor cells respond derive from lipoprotein signal peptides. using a novel bacterial killi ...201425249282
enterococcus faecalis zinc-responsive proteins mediate bacterial defence against zinc overload, lysozyme and oxidative stress.two enterococcus faecalis genes encoding the p-type atpase ef1400 and the putative sapb protein ef0759 were previously shown to be strongly upregulated in the presence of high concentrations of zinc. in the present work, we showed that a zn(2+)-responsive dna-binding motif (zim) is present in the promoter regions of these genes. both proteins were further studied with respect to their involvement in zinc homeostasis and invasion of the host. ef0759 contributed to intramacrophage survival by an a ...201425312746
antibacterial and antibiofilm activities of polysaccharides, essential oil, and fatty oil extracted from laurus nobilis growing in lebanon.to evaluate the antibacterial activity of the extracts of laurus nobilis against three gram-positive bacteria (staphylococcus aureus atcc 25923, enterococcus faecalis atcc 29212 and staphylococcus epidermidis cip 444) and two gram-negative bacteria (escherichia coli atcc 35218 and pseudomonas aeruginosa atcc 27853). also, the antibiofilm activity has been investigated against the biofilm produced by staphylococcus epidermidis cip 444.201425312182
novel in vitro methodology for induction of enterococcus faecalis biofilm on apical resorption areas.teeth with periapical lesion usually present external root resorption around the apical foramen. these areas facilitate adhesion and co-aggregation of microorganisms developing biofilms. up to the present moment, there is no methodology in the literature that enables the in vitro evaluation of endodontic irrigants and intracanal dressings on biofilms located in apical external root resorptions of human teeth.201425307923
monotreme lactation protein is highly expressed in monotreme milk and provides antimicrobial protection.monotremes (platypus and echidna) are the descendants of the oldest ancestor of all extant mammals distinguished from other mammals by mode of reproduction. monotremes lay eggs following a short gestation period and after an even briefer incubation period, altricial hatchlings are nourished over a long lactation period with milk secreted by nipple-less mammary patches located on the female's abdomen. milk is the sole source of nutrition and immune protection for the developing young until weanin ...201425245409
in vitro assessment of the recurrent doses of topical gaseous ozone in the removal of enterococcus faecalis biofilms in root canals.to evaluate the potential antibacterial effect of recurrent doses of topical gaseous ozone on the enterococcus faecalis biofilms0 growth in human root canals in vitro.201425244266
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