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engineering acetyl coenzyme a supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of saccharomyces cerevisiae.the energetic (atp) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. cytosolic acetyl coenzyme a (acetyl-coa) is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into saccharomyces cerevisiae, such as isoprenoids or lipids. in this yeast, synthesis of cytosolic acetyl-coa via acetyl-coa synthetase (acs) involves hydrolysis of atp to amp and pyrophosphat ...201425336454
central line associated blood stream infection rate after intervention and comparing outcome with national healthcare safety network and international nosocomial infection control consortium data.benchmarking of central line associated blood stream infection (clabsi) rates remains a problem in developing countries due to the variations in surveillance practices and/or infection risk as non-availability of national data.201425328774
selection of potential probiotic enterococcus faecium isolated from portuguese fermented food.four enterococcus faecium strains isolated from fermented products were evaluated for potential use as probiotic strains. in addition to efaafm gene, commonly found in e. faecium food isolates, none of the isolates possessed virulence genes and none had positive reactions for the production of tyramine, histamine, putrescine and cadaverine in the screening medium used. all of these four isolates proved to be resistant to 65 °c. e. faecium 119 did not show antimicrobial activity against any of th ...201425268323
a comparative study on sealing ability of mineral trioxide aggregate, calcium enriched cement and bone cement in furcal perforations.the aim of this study was to compare the bacterial leakage of mineral trioxide aggregate (mta), calcium enriched cement (cem), and bone cement (bc) as repair materials in furcal perforations.201425267149
antimicrobial activity of a sodium hypochlorite/etidronic acid irrigant solution.the aim of this study was to evaluate the antimicrobial activity of a 2.5% sodium hypochlorite (naocl)/9% etidronic acid (hebp) irrigant solution on enterococcus faecalis growing in biofilms and a dentinal tubule infection model.201425266466
in vitro evaluation of antibacterial effect of ah plus incorporated with quaternary ammonium epoxy silicate against enterococcus faecalis.the purpose of this study was to evaluate the in vitro antibacterial effect of ah plus (dentsply, detrey, konstanz, germany) incorporated with quaternary ammonium epoxy silicate (qaes) against enterococcus faecalis.201425260732
a widely used in vitro biofilm assay has questionable clinical significance for enterococcal endocarditis.biofilm formation may play an important role in the pathogenesis of infections caused by enterococcus faecalis, including endocarditis. most biofilm studies use a polystyrene dish assay to quantify biofilm biomass. however, recent studies of e. faecalis strains in tissue and animal models suggest that polystyrene dish results need to be interpreted with caution. we evaluated 158 clinical e. faecalis isolates using a polystyrene dish assay and found variation in biofilm formation, with many isola ...201425255085
intestinal translocation of clinical isolates of vancomycin-resistant enterococcus faecalis and esbl-producing escherichia coli in a rat model of bacterial colonization and liver ischemia/reperfusion injury.the objectives of this study were to develop a rat model of gastrointestinal colonization with vancomycin-resistant enterococcus faecalis (vre) and extended-spectrum beta-lactamase (esbl)-producing e. coli and to evaluate intestinal translocation to blood and tissues after total and partial hepatic ischemia. methods - we developed a model of rat colonization with vre and esbl-e coli. then we studied four groups of colonized rats: group i (with hepatic pedicle occlusion causing complete liver isc ...201425255079
measurement of cytokine levels in cerebrospinal fluid over time in neonatal enterococcal meningitis.enterococcus faecalis is rarely involved in neonatal meningitis. several studies have indicated that the cytokines related to bacterial infection may induce nerve cell damage; therefore, the cytokine levels in cerebrospinal fluid (csf) could represent a valuable hallmark for rapid recognition of the disease and evaluation of the degree of neurological involvement. we analyzed cytokine levels in the csf of a neonate with e. faecalis meningitis over time. tumor necrosis factor-α (tnf-α) tended to ...201425252071
antimicrobial efficacy of er,cr:ysgg laser-activated irrigation compared with passive ultrasonic irrigation and rinsendo(®) against intracanal enterococcus faecalis.the aim of this study was to evaluate the antibacterial efficacy of active irrigation techniques: erbium, chromium: yttrium, scandium, gallium, garnet (er,cr:ysgg) laser-activated irrigation (lai), passive ultrasonic irrigation (pui), rinsendo(®), and conventional syringe irrigation, against intracanal enterococcus faecalis.201425251217
antibacterial power of sodium hypochlorite combined with surfactants and acetic acid.the aim of this ex vivo study was to compare the antibacterial power of 1% naocl with 1% acetic acid, 5.25% naocl and two commercially available naocl modified with surfactants in bovine root dentin. a total of 120 dentin tubes prepared from intact bovine incisors were infected for 21 days with enterococcus faecalis and randomly divided into six groups as follows: 5.25%naocl; hypoclean; chlor-xtra; 1% naocl with 1% acetic acid; infected dentin tubes (positive control); and sterile dentin tubes ( ...201425250491
peritonitis in a continuous ambulatory peritoneal dialysis patient by two different species of enterococci: a rare finding.peritonitis in a continuous ambulatory peritoneal dialysis patient by two different species of enterococci is a rare condition. we report a case of peritonitis from which vancomycin sensitive enterococcus faecalis and vancomycin resistant enterococcusc faecium were isolated. it also emphasizes the effectiveness of linezolid for the treatment of vancomycin resistant enterococcal infection.201425249726
an abc transporter is required for secretion of peptide sex pheromones in enterococcus faecalis.enterococci are leading causes of hospital-acquired infection in the united states and continue to develop resistances to new antibiotics. many enterococcus faecalis isolates harbor pheromone-responsive plasmids that mediate horizontal transfer of even large blocks of chromosomal genes, resulting in hospital-adapted strains over a quarter of whose genomes consist of mobile elements. pheromones to which the donor cells respond derive from lipoprotein signal peptides. using a novel bacterial killi ...201425249282
development and evaluation of a quadruplex taq man real-time pcr assay for simultaneous detection of clinical isolates of enterococcus faecalis, enterococcus faecium and their vana and vanb genotypes.we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci.201425848524
the cell wall-targeting antibiotic stimulon of enterococcus faecalis.enterococcus faecalis is an opportunistic nosocomial pathogen that is highly resistant to a variety of environmental insults, including an intrinsic tolerance to antimicrobials that target the cell wall (cw). with the goal of determining the cw-stress stimulon of e. faecalis, the global transcriptional profile of e. faecalis og1rf exposed to ampicillin, bacitracin, cephalotin or vancomycin was obtained via microarrays. exposure to the β-lactams ampicillin and cephalotin resulted in the fewest tr ...201423755154
bacterial colonization of microbial biofilms in oral squamous cell carcinoma.the purpose of this prospective clinical study was to identify the bacterial spectra on the surface of oral squamous cell carcinomas (oscc) in comparison to oral mucosa of patients with a higher risk to emerge an oscc and a control group to determine their susceptibility to various common antibiotics.201423793404
antimicrobial activity of a uv-stable bacteriocin-like inhibitory substance (blis) produced by enterococcus faecium strain dsh20 against vancomycin-resistant enterococcus (vre) strains.the narrow spectrum of action of most bacteriocins is an important limitation for their application as antimicrobial agents. the current study describes a novel bacteriocin-like inhibitory substance (blis) that display extended spectrum antimicrobial activity against vancomycin-resistant enterococcus (vre) strains.201423838264
effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen.to study how products released from different bacteria in a deep carious lesion affect the metabolic activity of odontoblast-like cells and their ability to produce the major organic component of dentine, collagen 1.201423902462
isolation, identification, and antibiogram of enterococci isolated from patients with urinary tract infection.enterococci, though commensals in adult feces are important nosocomial pathogens. the most common nosocomial infection caused by these organisms is urinary tract infection.201424005591
reduction in enteroccocus faecalis counts - a comparison between rotary and reciprocating systems.to compare the chemomechanical reduction and regrowth of enterococcus faecalis between rotary and reciprocating systems in root canal preparation.201423980811
efficacy of endodontic applications of ozone and low-temperature atmospheric pressure plasma on root canals infected with enterococcus faecalis.this study aimed to compare the antimicrobial efficacy of low-temperature atmospheric pressure plasma (ltapp) design and gaseous ozone delivery system with 2.5% naocl on enterococcus faecalis in root canal walls and dentine tubules. the samples were divided into ltapp (n = 12), ozone (n = 12), naocl (positive control, n = 12) and saline (negative control, n = 6) groups. microbial samples were collected using paper points and dentin chips from root canals. antimicrobial efficacy was assessed by c ...201423980743
marked elevation of interleukin-6 in mild encephalopathy with a reversible splenial lesion (mers) associated with acute focal bacterial nephritis caused by enterococcus faecalis.this report describes two cases of mild encephalitis/encephalopathy with reversible splenial lesion (mers) associated with acute focal bacterial nephritis (afbn). the patients, who presented with fever and delirious behavior, exhibited hyponatremia and markedly elevated interleukin (il)-6 in cerebrospinal fluid (csf) and serum. enterococcus faecalis was detected in the urine culture. after ampicillin treatment, their consciousness improved without neurological sequelae. moreover, a diffusion-wei ...201423978488
american crows as carriers of vancomycin-resistant enterococci with vana gene.we studied the vana-carrying vancomycin-resistant enterococci (vre) isolated from american crows in the united states during the winter 2011/2012. faecal samples from crows were cultured selectively for vre and characterized. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) were used to examine epidemiological relationships of vana-containing vre. isolates were tested in vitro for their ability to horizontally transfer the vancomycin resistance trait. vre with the va ...201423919480
interdigitated silver-polymer-based antibacterial surface system activated by oligodynamic iontophoresis - an empirical characterization study.there is a pressing need to control the occurrences of nosocomial infections due to their detrimental effects on patient well-being and the rising treatment costs. to prevent the contact transmission of such infections via health-critical surfaces, a prophylactic surface system that consists of an interdigitated array of oppositely charged silver electrodes with polymer separations and utilizes oligodynamic iontophoresis has been recently developed. this paper presents a systematic study that em ...201423917746
endogenous endophthalmitis caused by enterococcus faecalis in a cat.a three-year-old male neutered domestic shorthair cat was presented for loss of vision associated with hyperthermia, lethargy and anorexia. ophthalmic examination revealed a bilateral panuveitis. cytological examination of aqueous and vitreous humours was performed and revealed a suppurative inflammation associated with numerous cocci. enterococcus faecalis was identified by bacterial culture from aqueous and vitreous humour. no primary infection site was identified. active uveitis resolved afte ...201424028653
emergence of enterococcus species in the infectious microorganisms cultured from patients with endophthalmitis in south korea.to investigate the microorganisms in culture-proven endophthalmitis and their susceptibilities to antimicrobial agents commonly used in south korea.201424072645
solidago, orthosiphon, birch and cranberry extracts can decrease microbial colonization and biofilm development in indwelling urinary catheter: a microbiologic and ultrastructural pilot study.plants extracts are used in urology to manage urinary tract infections. we aimed to evaluate the efficacy of a preparation with solidago, orthosiphon, birch and cranberry extracts (cistimev plus(®)) in reducing microbial colonization and biofilm development in patients with indwelling urinary catheters.201424092275
in vitro efficacy of various topical antimicrobial agents in different time periods from contamination to application against 6 multidrug-resistant bacterial strains isolated from burn patients.in vitro efficacy evaluation of eleven topical antimicrobials against multidrug-resistant (mdr) bacteria isolated from burn wounds of our patients.201424091332
apical extrusion of bacteria when using reciprocating single-file and rotary multifile instrumentation systems.to evaluate ex vivo, apical bacterial extrusion associated with two reciprocating single-file systems (waveone and reciproc) compared with a conventional multifile rotary system (biorace).201424111671
isolation and partial characterization of bacteria (pseudoalteromonas sp.) with potential antibacterial activity from a marine costal environment from new caledonia.marine bacteria are a rich source of bioactive metabolites. however, the microbial diversity of marine ecosystem still needs to be explored. the aim of this study was to isolate and characterize bacteria with antimicrobial activities from various marine coastal environment of new caledonia. we obtained 493 marine isolates from various environments and samples of which 63 (12.8%) presented an antibacterial activity against a panel of reference pathogenic strains (staphylococcus aureus, pseudomona ...201424106876
polymerase chain reaction for enterococcus faecalis in drain fluid: the first screening test for symptomatic colorectal anastomotic leakage. the appeal-study: analysis of parameters predictive for evident anastomotic leakage.with current diagnostic methods, the majority of patients with symptomatic colorectal anastomotic leakage(cal) is identified approximately 1 week after operation.the aim of this study is to determine whether real-time polymerase chain reaction (rt-pcr) for detection of escherichia coli and enterococcus faecalis on drain fluid can serve as a screening test for cal in the early postoperative phase.201424122105
antimicrobial activity of short- and medium-term applications of polyhexamethylene biguanide, chlorhexidine digluconate and calcium hydroxide in infected immature bovine teeth in vitro.to compare the antimicrobial activity of polyhexamethylene biguanide (prontosan wound gel, pr) and chlorhexidine digluconate (chx) after short- and medium-term application with the disinfection ability of calcium hydroxide (ca) in a model using immature bovine teeth.201424118966
hydrolysates of glycated and heat-treated peanut 7s globulin (ara h 1) modulate human gut microbial proliferation, survival and adhesion.evaluation of an effect of glycation of ara h 1 on proliferation and survival rate and adhesion of intestinal enterococcus faecalis, escherichia coli and lactobacillus acidophilus.201424118877
isolation and biological activities of an endophytic mortierella alpina strain from the antarctic moss schistidium antarctici.the antarctic endophytic fungus (strain ita1-ccma 952) was isolated from the moss schistidium antarctici found in admiralty bay, king george island, antarctica. strain ita1-ccma 952 was assigned to the specie mortierella alpina by phylogenetic analysis based on 18s rrna gene sequences. this strain produces high levels of polyunsaturated fatty acids (pufas), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3% of the total fatty acid content. fungal extrac ...201424126742
sunlight mediated inactivation mechanisms of enterococcus faecalis and escherichia coli in clear water versus waste stabilization pond water.escherichia coli and enterococci have been previously reported to differ in the mechanisms and conditions that affect their sunlight-mediated inactivation in waste stabilization ponds. this study was undertaken to further characterize these mechanisms, using simulated sunlight and single strains of laboratory-grown e. coli and enterococcus faecalis, with a focus on characterizing the contribution of exogenous reactive oxygen species to the inactivation process. we found that direct damage by uvb ...201424188579
antibacterial, physicochemical and mechanical properties of endodontic sealers containing quaternary ammonium polyethylenimine nanoparticles.to evaluate the antibacterial, physicochemical and mechanical properties of two endodontic sealers incorporating quaternary ammonium polyethylenimine (qpei) nanoparticles at concentrations of 1% and 2% (w/w).201424134748
vancomycin-resistant enterococci among haemodialysis patients in portugal: prevalence and molecular characterization of resistance, virulence and clonality.vancomycin-resistant enterococci (vre) among haemodialysis patients has increased rapidly and, to date, there is no report of this incidence in portugal.201424169320
a new antibacterial amino phenyl pyrrolidone derivative from a novel marine gliding bacterium rapidithrix thailandica.a recently described marine gliding bacterium rapidithrix thailandica strain tistr 1741 was isolated from biofilm specimen collected from the andaman sea in thailand. four liters fermentation broth of r. thailandica tistr 1741 cultivated in vy/2 medium were extracted with methanol to yield a novel amino phenyl pyrrolidone derivative compound (1) with antibacterial activities. the chemical structure and physico-chemical properties of 1 were investigated by spectrometry techniques. compound 1 exhi ...201424162950
activities of fosfomycin and rifampin on planktonic and adherent enterococcus faecalis strains in an experimental foreign-body infection model.enterococcal implant-associated infections are difficult to treat because antibiotics generally lack activity against enterococcal biofilms. we investigated fosfomycin, rifampin, and their combinations against planktonic and adherent enterococcus faecalis (atcc 19433) in vitro and in a foreign-body infection model. the mic/mbclog values were 32/>512 μg/ml for fosfomycin, 4/>64 μg/ml for rifampin, 1/2 μg/ml for ampicillin, 2/>256 μg/ml for linezolid, 16/32 μg/ml for gentamicin, 1/>64 μg/ml for va ...201424145537
nonclinical and clinical enterococcus faecium strains, but not enterococcus faecalis strains, have distinct structural and functional genomic features.certain strains of enterococcus faecium and enterococcus faecalis contribute beneficially to animal health and food production, while others are associated with nosocomial infections. to determine whether there are structural and functional genomic features that are distinct between nonclinical (nc) and clinical (cl) strains of those species, we analyzed the genomes of 31 e. faecium and 38 e. faecalis strains. hierarchical clustering of 7,017 orthologs found in the e. faecium pangenome revealed ...201424141120
microbiological findings of vulvovaginitis in prepubertal girls.to compare vaginal culture results between prepubertal girls with and without vulvovaginitis, and obtain an overview of the most commonly encountered microbes.201424191064
prevalence of the aminoglycoside phosphotransferase genes aph(3')-iiia and aph(3')-iia in escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus aureus isolates in austria.the aminoglycoside phosphotransferase aph(3')-iia primarily inactivates kanamycin and neomycin, whilst aph(3')-iiia also inactivates amikacin. the aim of this study was to determine the frequency of both resistance genes in major human pathogens to obtain their baseline prevalence in the gene pool of these bacterial populations in austria. in total, 10 541 escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus ...201424194558
photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen.treatment of infected teeth presents two major challenges: persistence of the bacterial-biofilm within root canals after treatment and compromised structural integrity of the dentin hard-tissue. in this study bioactive polymeric chitosan nanoparticles functionalized with rose-bengal, csrbnp were developed to produce antibiofilm effects as well as stabilize structural-integrity by photocrosslinking dentin-collagen. csrbnp were less toxic to fibroblasts and had significant antibacterial activity e ...201424200522
friends group: clinical and microbiological characteristics of post-filtering surgery endophthalmitis.to study the clinical and microbiological characteristics as well as the prognostic factors for post-filtering surgery endophthalmitis.201424248809
boosted rat natural xenoantibodies cross-react with enterococcus faecalis by targeting melibiose and l-rhamnose.natural antibodies include a subset described as xenoantibodies considered to be directed at microorganisms and also cross-react with antigens of unrelated species. in this study, we generated t-cell-independent (ti) and t-cell-dependent (td) xenoantibodies in lewis rats with hamster and pig blood injections. ti anti-hamster and anti-pig igm and igg xenoantibodies cross-reacted with enterococcus faecalis but not with escherichia coli isolated from the blood of lewis rats after cecal ligation and ...201424246417
lipoteichoic acid of streptococcus mutans interacts with toll-like receptor 2 through the lipid moiety for induction of inflammatory mediators in murine macrophages.streptococcus mutans is a pathogenic gram-positive bacterium that is closely associated with dental caries and subsequent pulpal inflammation. although lipoteichoic acid (lta) is considered a major virulence factor of gram-positive bacteria, little is known about the innate immunity to s. mutans lta. in this study, we purified lta from s. mutans (sm.lta) through n-butanol extraction, hydrophobic interaction column chromatography, and ion-exchange column chromatography to investigate its immunolo ...201424216318
biofilm mediates enterococcus faecalis adhesion, invasion and survival into bovine mammary epithelial cells.we proposed in this study that during intramammary infection, biofilm formation may facilitate adherence and colonization of enterococcus faecalis to mammary gland epithelium. this was established by comparing six different ent. faecalis isolates with different biofilm-forming profiles for their adhesive, invasive and survival capabilities to bovine mammary epithelial cell line (mac-t). our results showed increased ability of the biofilm-producer ent. faecalis strains to adhere, invade and survi ...201424224825
systematic evaluation of commercial susceptibility testing methods for determining the in vitro activity of daptomycin versus staphylococcus aureus and enterococci.we systematically evaluated 5 methods for testing daptomycin versus 48 enterococcus faecalis, 51 enterococcus faecium, and 50 staphylococcus aureus isolates using (i and ii) broth microdilution (bmd) with 50-mg/liter calcium medium supplementation (reference method) and 30-mg/liter calcium medium supplementation (bmd30 method), (iii) etest, and (iv and v) microscan panel 33 using 2 methods to prepare the bacterial inoculum (microscan turbidity and microscan prompt). isolates were categorized as ...201424648560
daptomycin activity tested against 164457 bacterial isolates from hospitalised patients: summary of 8 years of a worldwide surveillance programme (2005-2012).we report the results of 8 years (2005-2012) of the daptomycin surveillance programme worldwide. consecutive non-duplicate bacterial isolates (prevalence design) were collected from patients with documented infections in 410 medical centres and were susceptibility tested by reference broth microdilution methods. a total of 164457 gram-positive isolates were evaluated, including 97542 staphylococcus aureus, 21413 coagulase-negative staphylococci (cons), 29619 enterococci and 15883 β-haemolytic st ...201424636430
green synthesis of silver nanoparticles using alternanthera dentata leaf extract at room temperature and their antimicrobial activity.a green rapid biogenic synthesis of silver nanoparticles agnps using alternanthera dentata (a. dentata) aqueous extract was demonstrated in this present study. the formation of silver nanoparticles was confirmed by surface plasmon resonance (spr) at 430nm using uv-visible spectrophotometer. the reduction of silver ions to silver nanoparticles by a. dentata extract was completed within 10min. synthesized nanoparticles were characterized using uv-visible spectroscopy; fourier transformed infrared ...201424632169
drug-resistant and hospital-associated enterococcus faecium from wastewater, riverine estuary and anthropogenically impacted marine catchment basin.enterococci, ubiquitous colonizers of humans and other animals, play an increasingly important role in health-care associated infections (hais). it is believed that the recent evolution of two clinically relevant species, enterococcus faecalis and enterococcus faecium occurred in a big part in a hospital environment, leading to formation of high-risk enterococcal clonal complexes (hireccs), which combine multidrug resistance with increased pathogenicity and epidemicity. the aim of this study was ...201424629030
antibacterial effect of iranian green-tea-containing mouthrinse vs chlorhexidine 0.2%: an in vitro study.considering the antioxidant, anti-inflammatory and antimicrobial properties of green tea, this study aimed to evaluate the antibacterial effect of mouthrinses containing green tea extract vs 0.2% chlorhexidine on selected microorganisms in vitro.201424624389
inactivation of airborne enterococcus faecalis and infectious bursal disease virus using a pilot-scale ultraviolet photocatalytic oxidation scrubber.high microbial concentrations and emissions associated with livestock houses raise health and environmental concerns. a pilot-scale ultraviolet photocatalytic oxidation (uv-pco) scrubber was tested for its efficacy to inactivate aerosolized enterococcus faecalis and infectious bursal disease virus (ibdv). microbial reduction was determined by the difference in microbial concentrations measured in the upstream and downstream isolators that were connected to the two ends of the uv-pco scrubber. tw ...201424620401
spread of multidrug-resistant enterococcus faecalis within the household setting.advances in veterinary medicine have resulted in the survival of many animals with severe illness or infectious diseases. in addition, increased usage of antimicrobial agents for veterinary purposes has contributed to the worldwide problem of increasing antimicrobial resistance. the objective of this study was to contribute to better understand the potential and implications for the spread of antimicrobial-resistant enterococci between pets receiving antimicrobial treatments and their owners. th ...201424617521
linezolid resistance in vancomycin-resistant enterococcus faecalis and enterococcus faecium isolates in a brazilian hospital. 201424614366
effects of intracanal irrigant mtad combined with nisin at sub-minimum inhibitory concentration levels on enterococcus faecalis growth and the expression of pathogenic genes.exposure to antibiotics is considered to be the major driver in the selection of antibiotic-resistant bacteria and may induce diverse biological responses in bacteria. mtad is a common intracanal irrigant, but its bactericidal activity remains to be improved. previous studies have indicated that the antimicrobial peptide nisin can significantly improve the bactericidal activity of mtad against enterococcus faecalis. however, the effects of mtad and its modification at sub-minimum inhibitory conc ...201424603760
antibacterial properties and mode of action of new triaryl butene citrate compounds.the aim of this study was to evaluate the antibacterial activity of newly synthesized triaryl butene analogues of tamoxifen. several compounds were synthesized and converted to citrate salts to ensure greater solubility. four compounds showed significant antibacterial activity at micromolar concentrations against gram-positive and gram-negative foodborne pathogens including listeria monocytogenes, listeria ivanovii, enterococcus faecalis, staphylococcus aureus and escherichia coli. two compounds ...201424602786
performance of the eucast disk diffusion method, the clsi agar screen method, and the vitek 2 automated antimicrobial susceptibility testing system for detection of clinical isolates of enterococci with low- and medium-level vanb-type vancomycin resistance: a multicenter study.different antimicrobial susceptibility testing methods to detect low-level vancomycin resistance in enterococci were evaluated in a scandinavian multicenter study (n=28). a phenotypically and genotypically well-characterized diverse collection of enterococcus faecalis (n=12) and enterococcus faecium (n=18) strains with and without nonsusceptibility to vancomycin was examined blindly in danish (n=5), norwegian (n=13), and swedish (n=10) laboratories using the eucast disk diffusion method (n=28) a ...201424599985
comparative evaluation of push-out bond strength of three endodontic sealers with and without amoxicillin-an invitro study.the purpose of this study was to evaluate the sealing ability of three different root canal sealers with and without amoxicillin, using a push-out bond strength test.201424596782
antibacterial substances from marine algae isolated from jeddah coast of red sea, saudi arabia.marine algae are known to produce a wide variety of bioactive secondary metabolites and several compounds have been derived from them for prospective development of novel drugs by the pharmaceutical industries. however algae of the red sea have not been adequately explored for their potential as a source of bioactive substances. in this context ulva reticulata, caulerpa occidentalis, cladophora socialis, dictyota ciliolata, and gracilaria dendroides isolated from red sea coastal waters of jeddah ...201424596500
etiological factors of infections in diabetic foot syndrome - attempt to define optimal empirical therapy.diabetic foot syndrome (dfs) represents one of the most frequent reasons for lower limb amputation in developed countries. in most cases, it is associated with bacterial infection, requiring optimal antibiotic therapy.201424596002
analysis of the promoters involved in enterocin as-48 expression.the enterocin as-48 is the best characterized antibacterial circular protein in prokaryotes. it is a hydrophobic and cationic bacteriocin, which is ribosomally synthesized by enterococcal cells and post-translationally cyclized by a head-to-tail peptide bond. the production of and immunity towards as-48 depend upon the coordinated expression of ten genes organized in two operons, as-48abc (where genes encoding enzymes with processing, secretion, and immunity functions are adjacent to the structu ...201424594763
the antibacterial activity, antioxidant activity and selectivity index of leaf extracts of thirteen south african tree species used in ethnoveterinary medicine to treat helminth infections.diseases caused by bacteria remain a major challenge globally and particularly in sub-saharan africa. the plants used in this study have been used in south africa to treat helminth infections in livestock and humans. in a previous study we found a correlation between antifungal and anthelmintic activity in some cases. in this study we examined other potential uses of these thirteen plant species by determining the antibacterial and antioxidant activity of the leaf acetone extracts.the antibacter ...201424589020
glycopeptide use is associated with increased mortality in enterococcus faecalis bacteraemia.enterococci are an important cause of nosocomial and community-acquired infections, with bacteraemia being one of the most common presentations. although inappropriate antimicrobial therapy has been associated with poorer outcomes in enterococcus faecalis (ef) bacteraemia, the impact of antimicrobial choice, namely β-lactam versus glycopeptide therapy, has not been well described. we sought to determine whether choice of antibiotic affects patient outcomes in ef bacteraemia.201424744303
passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against enterococcus faecalis.chemomechanical procedures can be used to eliminate bacteria from root canals. however, detectable bacteria sometimes remain because of the complexity of the root canal system. endodontic passive ultrasonic irrigation (pui) with hydrogen peroxide (h2o2) may be a promising option for increasing bactericidal hydroxyl radical (ho•) generation. in this in vitro experiment, we examined the effects of ho• generated using pui and a low concentration of h2o2. an ultrasonic tip was submerged in 0.45 mol/ ...201424739706
comparison of the antibacterial effect of sodium hypochlorite and aloe vera solutions as root canal irrigants in human extracted teeth contaminated with enterococcus faecalis.the main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. sodium hypochlorite has excellent antibacterial properties, but also some negative features.201424738089
an in vitro comparison of furcal perforation repaired with pro-root mta and new endodontic cement in primary molar teeth- a microleakage study.one of the most challenging procedural accidents during pulpotomy of primary molars is furcal perforation. to prevent bacterial invasion, the perforation site should be sealed as soon as possible.201424738087
occurrence of enterococcus species with virulence markers in an urban flow-influenced tropical recreational beach.median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<0.0001) with increasing proximity to river influx. the difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. virulence genes cyl, esp, gele and asa were detected at varying frequencies (7.32 ...201424725825
effects of resveratrol on gut microbiota and fat storage in a mouse model with high-fat-induced obesity.recent studies have investigated the anti-obesity effect of resveratrol, but the pathways through which resveratrol resists obesity are not clear. in the present study, we hypothesize that resveratrol exerts anti-obesity effects that are likely mediated by mechanisms of regulating gut microbes, and in turn, improving fat storage and metabolism. gut microbes, glucose and lipid metabolism in high-fat diet (hf) mice in vivo are investigated after resveratrol treatment. several biochemical markers a ...201424722352
structural elucidation of srta enzyme in enterococcus faecalis: an emphasis on screening of potential inhibitors against the biofilm formation.enterococcus faecalis is a pathogenic gram-positive bacterium, which mainly infects humans through urinary tract infections. srta is an essential enzyme for survival of e. faecalis, and inhibition of this particular enzyme will reduce the virulence of biofilm formation. it is proved to be associated with the microbial surface protein embedded signal transduction mechanism and promising as a suitable anti-microbial drug target for e. faecalis. the present work gives an inclusive description of sr ...201424718729
enhanced removal of enterococcus faecalis biofilms in the root canal using sodium hypochlorite plus photon-induced photoacoustic streaming: an in vitro study.the purpose of this study was to determine the effectiveness of laser-activated irrigation by photon-induced photoacoustic streaming (pips) using er:yag laser energy in decontaminating heavily colonized root canal systems in vitro.201424717113
impact of bacterial infections on aging and cancer: impairment of dna repair and mitochondrial function of host cells.the commensal floras that inhabit the gastrointestinal tract play critical roles in immune responses, energy metabolism, and even cancer prevention. pathogenic and out of place commensal bacteria, can however have detrimental effects on the host, by introducing genomic instability and mitochondrial dysfunction, which are hallmarks of both aging and cancer. helicobacter pylori and enterococcus faecalis are bacteria of the gastrointestinal tract that have been demonstrated to affect these two hall ...201424704713
colonization of enterococcus faecalis in a new sio/sio(2)-microtube in vitro model system as a function of tubule diameter.endodontic pathogens can penetrate deep into dentinal tubules and therefore survive the chemo-mechanical disinfection procedures. bacterial penetration has been mainly studies using sliced infected human teeth which, besides creating artifacts, can hinder the observation of the inner tubules due to the dense and opaque dentin structure. the aim of the present study was to develop a standardized dentin model by using artificial sio/sio2-microtubes of different diameters and lengths to test the pe ...201424703548
an in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against enterococcus faecalis.enterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic and acquired resistance to a variety of antibiotics. development of new antibiotics is limited, and pathogens continually generate new antibiotic resistance. many researchers aim to identify strategies to effectively kill this drug-resistant pathogen. here, we evaluated the effect of the antimicrobial peptide nisin on the antibacterial activities of 18 antibiotics against e. faecal ...201424586598
enterococcus infection biology: lessons from invertebrate host models.the enterococci are commensals of the gastrointestinal tract of many metazoans, from insects to humans. while they normally do not cause disease in the intestine, they can become pathogenic when they infect sites outside of the gut. recently, the enterococci have become important nosocomial pathogens, with the majority of human enterococcal infections caused by two species, enterococcus faecalis and enterococcus faecium. studies using invertebrate infection models have revealed insights into the ...201424585051
effects of single- and multi-strain probiotics on biofilm formation and in vitro adhesion to bladder cells by urinary tract pathogens.there is increasing evidence that probiotic bacteria can inhibit and/or prevent urinary tract infections. possible mechanisms include prevention of adhesion of pathogens to the bladder epithelium and inhibition of biofilm formation. currently there is interest in the comparative efficacy of single probiotics vs. strain mixtures. we have therefore tested the inhibitory activity of four single probiotics and four probiotic mixtures towards the urinary tract pathogens escherichia coli nctc 9001 and ...201424583095
photodynamic therapy versus ultrasonic irrigation: interaction with endodontic microbial biofilm, an ex vivo study.photodynamic therapy was introduced as an adjuvant to conventional chemo-mechanical debridement during endodontic treatment to overcome the persistence of biofilms. the aim of this study was to evaluate the ability of photodynamic therapy (pdt) to disrupt an experimental microbial biofilm inside the root canal in a clinically applicable working time.201424583092
recombinant human lipocalin 2 acts as an antibacterial agent to prevent platelet contamination.bacterial contamination of platelet products is the major infectious risk in blood transfusion medicine, which can result in life-threatening sepsis in recipient. lipocalin 2 (lcn2) is an iron-sequestering protein in the antibacterial innate immune response, which inhibit bacterial growth. this study was aimed to evaluate the antibacterial property of lcn2 in preventing bacterial contamination of platelets.201424580532
microwave-assisted preparation of azachalcones and their n-alkyl derivatives with antimicrobial activities.two new azachalcones were prepared by both claisen-schmidt condensation and a simple environmentally trendy microwave-assisted method. ten new n-alkyl (c6,8,10,12,14)-substituted azachalconium bromides (3a-e, 4a-e) were prepared from compounds 1 and 2 with corresponding alkyl halides. the antimicrobial activities of all the compounds were tested against enterococcus faecalis, yersinia pseudotuberculosis, pseudomonas aeruginosa, escherichia coli and staphylococcus aureus micro-organisms.201424571646
daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery.antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. in addition, daptomycin has been used recently against broad spectrum gram-positive organisms. the goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (pmma) bone cement and evaluate its feasibility for clinical use. daptomycin (0.5, 1, or 2 g) was premixed with 40 g ...201424571555
genome sequencing reveals the environmental origin of enterococci and potential biomarkers for water quality monitoring.enterococci are common members of the gut microbiome and their ease of culturing has facilitated worldwide use as indicators of fecal pollution of waters. however, enterococci were recently shown to persist in environmental habitats, often in the absence of fecal input, potentially confounding water quality assays. toward resolving this issue and providing a more complete picture of natural enterococci diversity, 11 isolates of enterococcus faecalis recovered from freshwater watersheds (environm ...201424571132
cell-recycle continuous fermentation of enterococcus faecalis rky1 for economical production of lactic acid by reduction of yeast extract supplementation.both lactic acid productivity and cell growth were linearly correlated with yeast extract supplementation in batch fermentation. during conventional continuous operation, although fresh feed was introduced into the bioreactor with a significantly low dilution rate (0.04 h(-1)), the amount of yeast extract employed was not enough to maintain the growth of microorganism. however, when the fresh feed contained 100 g/l glucose and 2 g/l yeast extract during cell-recycle continuous operation at a dil ...201424561722
fabrication and characterization of bioactive and antibacterial composites for dental applications.there is an increasing clinical need to design novel dental materials that combine regenerative and antibacterial properties. in this work the characterization of a recently developed sol-gel-derived bioactive glass ceramic containing silver ions (ag-bg) is presented. the microstructural characteristics, ion release profile, zeta potential value and changes in weight loss and ph value as a function of the immersion time of ag-bg in tris buffer are evaluated. ag-bg is also incorporated into natur ...201424802300
complete genome sequencing and comparative analysis of the linezolid-resistant enterococcus faecalis strain deng1.genome level analysis of bacterial strains provides information on genetic composition and resistance mechanisms to clinically relevant antibiotics. to date, whole genome characterization of linezolid-resistant enterococcus faecalis isolated in the clinic is lacking. in this study, we report the entire genome sequence, genomic characteristics and virulence factors of a pathogenic e. faecalis strain, deng1. our results showed considerable differences in genomic characteristics and virulence facto ...201424800692
colloidal gold-based immunochromatographic strip test compromising optimised combinations of anti-s. suis capsular polysaccharide polyclonal antibodies for detection of streptococcus suis.a rapid diagnosis kit that detects streptococcus suis (s. suis) antigens from urine with an immunochromatographic stripe (ics) test was developed in this study. the ics test was produced using colloidal gold coated with polyclonal antibodies (pabs) against s. suis. the pabs were developed from rabbits immunised with s. suis serotype 2 capsular polysaccharides (cps). development of the pabs was investigated to establish their binding to cps and to determine the maximum sensitivity of two combinat ...201424800681
impact of different antimicrobial therapies on clinical and fiscal outcomes of patients with bacteremia due to vancomycin-resistant enterococci.vancomycin-resistant enterococci (vre) are a growing health problem, and uncertainties exist regarding the optimal therapy for bloodstream infection due to vre. we conducted systematic comparative evaluations of the impact of different antimicrobial therapies on the outcomes of patients with bloodstream infections due to vre. a retrospective study from january 2008 to october 2010 was conducted at detroit medical center. unique patients with blood cultures due to vre were included and reviewed. ...201424798267
enterococcus faecalis bacteremia and mitral valve endocarditis under dabigatran for stroke prevention. 201424794060
antibacterial activity of kaolinite/nanotio2 composites in relation to irradiation time.the paper addresses laboratory preparation and antibacterial activity testing of kaolinite/nanotio2 composite in respect of the daylight irradiation time. kaolinite/nanotio2 composites with 20 and 40 wt% of tio2 were laboratory prepared, dried at 105 °c and calcined at 600 °c. the calcination caused transformation of kaolinite to metakaolinite and origination of the metakaolinite/nanotio2 composite. x-ray powder diffraction, raman and ftir spectroscopic methods revealed titanium dioxide only in ...201424792569
bacterial leakage in root canals filled with ah plus and dentine bonding agents.the aim of this study was to compare the efficacy of different dentine adhesives in delaying the coronal bacterial leakage of enterococcus faecalis in filled root canals. materials and methods. ninety-five lower incisors of patients >65 years of age were instrumented using the protaper system and were irrigated with 1 ml of 2.5% sodium hypochlorite (naocl) alternated with 1 ml 17% edta between each file change. final irrigation was performed with 5 ml of 17% edta and then flushed with 5 ml of di ...201424791609
diverse outcomes of photodynamic antimicrobial chemotherapy on five enterococcus faecalis strains.in the present study, the effectiveness of photodynamic antimicrobial chemotherapy (pact) was evaluated on planktonic cells and biofilms of five enterococcus faecalis clinical isolates.201424784432
study comparing the effectiveness of chlorhexidine, calcium hydroxide and linezolid based medicaments against enterococcus faecalis.this study evaluated the efficacy of 2% chlorhexidine (cx), calcium hydroxide (ch), vitapex® (vp), linezolid (lz), a combination of lz with ch (lc) against enterococcus faecalis (ef).201424783147
correlation between biofilm formation and gele, esp, and agg genes in enterococcus spp. clinical isolates. 201424782231
health evaluation and survey of zoonotic pathogens in free-ranging capybaras (hydrochoerus hydrochaeris).capybaras (hydrochoerus hydrochaeris) are the world's largest rodents and play an epidemiologic role in the transmission of zoonotic pathogens, including the causative agents of brazilian spotted fever, leptospirosis, and others. we surveyed the health of 31 free-ranging capybaras at the alberto löfgren state park, são paulo, brazil using a variety of diagnostic methods. hematology and serum chemistry were consistent with mild malnutrition and parasitism but did not indicate severe physiologic i ...201424779462
evaluation of the presence of enterococcus faecalis in root cementum: a confocal laser scanning microscope analysis.the aim of this study is to address the cause of persistent infection of root cementum by enterococcus faecalis.201424778505
evaluation of the novel combination of daptomycin plus ceftriaxone against vancomycin-resistant enterococci in an in vitro pharmacokinetic/pharmacodynamic simulated endocardial vegetation model.daptomycin has demonstrated synergy with β-lactams against enterococcus faecium and this combination has been used successfully to treat infections refractory to daptomycin. we investigated daptomycin alone and combined with ceftriaxone against vancomycin-resistant enterococci (vre) in an in vitro pharmacokinetic/pharmacodynamic simulated endocardial vegetation (sev) model.201424777900
a newly isolated probiotic enterococcus faecalis strain from vagina microbiota enhances apoptosis of human cancer cells.this study aimed to describe probiotic properties and bio-therapeutic effects of newly isolated enterococcus faecalis from the human vaginal tract.201424775273
pyruvate formate-lyase is essential for fumarate-independent anaerobic glycerol utilization in the enterococcus faecalis strain w11.although anaerobic glycerol metabolism in enterococcus faecalis requires exogenous fumarate for nadh oxidation, e. faecalis strain w11 can metabolize glycerol in the absence of oxygen without exogenous fumarate. in this study, metabolic end product analyses and reporter assays probing the expression of enzymes involved in pyruvate metabolism were performed to investigate this fumarate-independent anaerobic metabolism of glycerol in w11. under aerobic conditions, the metabolic end products of w11 ...201424769696
evaluation of antimicrobial activity of silver nanoparticles for carboxymethylcellulose film applications in food packaging.in this study, silver nanoparticles were prepared and incorporated into carboxymethylcellulose films to evaluate the antimicrobial activity for food packaging applications. the techniques carried out for material characterization were: infrared spectroscopy and thermal analysis for the silver nanoparticles and films, as well as particle size distribution for the nanoparticles and water vapor permeability for the films. the antimicrobial activity of silver nanoparticles prepared by casting method ...201424758059
transcriptomic and functional analysis of nacl-induced stress in enterococcus faecalis.the robust physiology of enterococcus faecalis facilitates tolerance to various stresses. we here report the transcriptional response of e. faecalis v583 to growth in the presence of 6.5% nacl. among the early responses observed was an immediate down-regulation of mscl, accompanied by an up-regulation of genes predicted to be involved in uptake of extracellular potassium and glycine betaine. the high nacl concentration also induced expression of chaperons and cell envelope related traits, such a ...201424755907
antimicrobial and immunomodulatory activities of pr-39 derived peptides.the porcine cathelicidin pr-39 is a host defence peptide that plays a pivotal role in the innate immune defence of the pig against infections. besides direct antimicrobial activity, it is involved in immunomodulation, wound healing and several other biological processes. in this study, the antimicrobial- and immunomodulatory activity of pr-39, and n- and c-terminal derivatives of pr-39 were tested. pr-39 exhibited an unexpected broad antimicrobial spectrum including several gram positive strains ...201424755622
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