Publications

one-pot biogenic fabrication of silver nanocrystals using quisqualis indica: effectiveness on malaria and zika virus mosquito vectors, and impact on non-target aquatic organisms.	currently, mosquito vector control is facing a number of key challenges, including the rapid development of resistance to synthetic pesticides and the recent spread of aggressive arbovirus outbreaks. the biosynthesis of silver nanoparticles (agnps) is currently considered an environmental friendly alternative to the employ of pyrethroids, carbamates and microbial agents (e.g. bacillus thuringiensis var. israelensis), since agnps are easy to produce, effective and stable in the aquatic environmen ...	2016	27491031
receptors are affected by selection with each bacillus thuringiensis israelensis cry toxin but not with the full bti mixture in aedes aegypti.	bacillus thuringiensis israelensis (bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. by using rna sequencing, we investigated change in gene transcription level and polymorphism variations associated with resistance to each bti cry toxin and to the full bti toxin mixture in the dengue vector aedes aegypti. the up-regulation of genes related to chitin metabolism in a ...	2016	27418233
the use of aedes aegypti larvae attractants to enhance the effectiveness of larvicides.	aedes aegypti (l.) is an important dengue, chikungunya, and yellow fever vector. immature stages of this species inhabit human-made containers placed in residential landscapes, and the application of larvicides inside containers that cannot be eliminated is still considered a priority in control programs. larvicidal efficacy is influenced by several factors, including the formulation used, the water quality, and the susceptibility of larvae, among others. if an attractant can be incorporated int ...	2016	26922177
identification and characterization of ige-binding tropomyosins in aedes aegypti.	the mosquito aedes aegypti is a potential source of important clinically relevant allergens. however, the allergenicity and cross-reactivity of most of these has not been fully described.	2016	27355916
dibenzo-α-pyrones: a new class of larvicidal metabolites against aedes aegypti from the endophytic fungus hyalodendriella sp. ponipodef12.	in our search for new agrochemicals from endophytic fungi, the crude extract of the endophytic hyalodendriella sp. ponipodef12 associated with the hybrid 'neva' of populus deltoides marsh × p. nigra l. was found to possess larvicidal activity against aedes aegypti.	2016	27862895
[pathogenicity of aedes aegypti l. mosquito densonucleosis virus to larvae of other species of blood-sucking mosquitoes].	laboratory studies established that a. aegypti mosquito virus of densonucleosis was infectious for larvae of a. caspius dorsalis, a. vexans, a. cinereus and c. pipiens pipiens caught in natural water bodies. death rates of aedes and culex larvae experimentally were 46.3% on the average, while in control groups 19.7%. the external appearance of virus-infected larvae and pathological changes in nuclei of the cells of the fat body were identical to those previously described in a. aegypti larvae af ...	2016	1220247
origin of the zika virus revealed: a historical journey across the world.	zika virus (zikv) is an arbovirus within the flaviviridae family, the recent spread of which has promoted public concern.	2016	27650823
impact of autocidal gravid ovitraps on chikungunya virus incidence in aedes aegypti (diptera: culicidae) in areas with and without traps.	puerto rico detected the first confirmed case of chikungunya virus (chikv) in may 2014 and the virus rapidly spread throughout the island. the invasion of chikv allowed us to observe aedes aegypti (l.) densities, infection rates, and impact of vector control in urban areas using cdc autocidal gravid ovitraps (ago traps) for mosquito control over several years. because local mosquitoes can only get the virus from infectious residents, detecting the presence of virus in mosquitoes functions as a p ...	2016	28031347
chikungunya in the americas: recommendations and conclusions.	discovered in 1953, chikungunya virus (chikv) circulated in africa and southeast asia, with periodic outbreaks, for many years. highly efficient transmission following a genetic mutation of the virus in 2005 caused its global spread. associated with significant morbidity, chikv creates a large public health burden, and despite various efforts, there are currently no licensed vaccines nor specific treatments. to garner a better understanding of the virus, identify gaps in knowledge, and guide the ...	2016	27920183
bridging the gap between experimental data and model parameterization for chikungunya virus transmission predictions.	chikungunya virus (chikv) has experienced 2 major expansion events in the last decade. the most recently emerged sublineage (ecsa-v) was shown to have increased efficiency in a historically secondary vector, aedes albopictus, leading to speculation that this was a major factor in expansion. subsequently, a number of experimental studies focused on the vector competence of chikv, as well as transmission modeling efforts. mathematical models have used these data to inform their own investigations, ...	2016	27920175
perspectives and challenges in entomological risk assessment and vector control of chikungunya.	chikungunya virus (chikv) is primarily spread by the aedes aegypti and aedes albopictus mosquito vectors. because there is no licensed vaccine for chikv, identifying ways to reduce or eliminate mosquito populations is the most effective strategy to immediately halt transmission to man. strategies to assess the entomological risk and to control the vector are absolutely crucial to demolishing the rise of chikv. this review provides perspectives in entomological risk assessment and vector control, ...	2016	27920174
invasiveness of aedes aegypti and aedes albopictus and vectorial capacity for chikungunya virus.	in this review, we highlight biological characteristics of aedes aegypti and aedes albopictus, 2 invasive mosquito species and primary vectors of chikungunya virus (chikv), that set the tone of these species' invasiveness, vector competence, and vectorial capacity (vc). the invasiveness of both species, as well as their public health threats as vectors, is enhanced by preference for human blood. vector competence, characterized by the efficiency of an ingested arbovirus to replicate and become i ...	2016	27920173
chikungunya: its history in africa and asia and its spread to new regions in 2013-2014.	chikungunya virus (chikv) is transmitted by aedes aegypti and aedes albopictus mosquitoes and causes febrile illness with severe arthralgia in humans. there are 3 circulating chikv genotypes, asia, east/central/south africa, and west africa. chikv was first reported in 1953 in tanzania, and up until the early 2000s, a few outbreaks and sporadic cases of chikv were mainly reported in africa and asia. however, from 2004 to 2005, a large epidemic spanned from kenya over to the southwestern indian o ...	2016	27920169
indoor development of aedes aegypti in germany, 2016.	in spring 2016, a german traveller returning from martinique cultivated imported plant offsets in her home, and accidentally bred aedes aegypti. thirteen adult mosquito specimens submitted for identification and the traveller were tested for zika, dengue and chikungunya virus infections, with negative results. the detection of ae. aegypti by the 'mueckenatlas' project demonstrates the value of this passive surveillance scheme for potential public health threats posed by invasive mosquitoes in ge ...	2016	27918261
[recommendations for the surveillance of aedes aegypti].	diseases caused by arboviruses transmitted by aedes aegypti, such as dengue, chikungunya and zika, continue to rise in annual incidence and geographic expansion. a key limitation for achieving control of a. aegypti has been the lack of effective tools for monitoring its population, and thus determine what control measures actually work. surveillance of a. aegypti has been based mainly on immature indexes, but they bear little relation to the number of mosquito females, which are the ones capable ...	2016	27869394
testing of visual and chemical attractants in correlation with the development and field evaluation of an autodissemination station for the suppression of aedes aegypti and aedes albopictus in florida.	three exotic mosquito-borne pathogens-dengue, chikungunya, and zika viruses-transmitted by aedes albopictus and ae. aegypti have undergone dramatic global expansion in recent years. the control of vector populations and minimizing bites from these vectors are the primary methods of reducing risk of transmission of these viruses to humans. however, ae. albopictus and ae. aegypti are notoriously challenging to control through conventional chemical means, due primarily to difficulties in applying p ...	2016	27802398
knockdown resistance mutations in aedes aegypti (diptera: culicidae) from puerto rico.	permethrin resistance is widespread in aedes aegypti (l.), the main dengue, zika, and chikungunya virus vector in latin america and the caribbean. a common mechanism of resistance to pyrethroids-knockdown resistance (kdr)-is conferred through mutations in the insect's voltage-dependent sodium channel. in this mosquito, around 10 replacement substitutions in the voltage-gated sodium channel gene (vgsc) have been reported in pyrethroid-resistant strains. two of these mutations, named ile1,016 and ...	2016	27493252
chikugunya and zika virus dissemination in the americas: different arboviruses reflecting the same spreading routes and poor vector-control policies.	this review gathers the most recent investigations about chikungunya and zika viruses in america and would help in creating new research approaches.	2016	27472289
two novel epistatic mutations (e1:k211e and e2:v264a) in structural proteins of chikungunya virus enhance fitness in aedes aegypti.	expansion of chikv outbreaks with appearance of novel mutations are reported from many parts of the world. two novel mutations viz. e1:k211e and e2:v264a in background of e1:226a are recently identified from aedes aegypti dominated areas of india. in this study, the role of these mutations in modulation of infectivity, dissemination and transmission by two different aedes species was studied. mutations were sequentially constructed in chikv genome and female ae. aegypti and aedes albopictus mosq ...	2016	27423270
distribution of aedes mosquitoes in the kilimanjaro region of northern tanzania.	little is known about the presence and distribution of aedes mosquitoes in northern tanzania despite the occurence of viruses transmitted by these mosquitoes such as chikungunya virus (chikv) and dengue virus (denv) in the region. adult and larval mosquitoes were collected from rural and urban settings across a wide range of altitudes in the kilimanjaro region using the mosquito magnet co2 trap for collection of adults and old tires for breeding of larvae. polymerase chain reaction assays were p ...	2016	27376502
evidence for natural vertical transmission of chikungunya viruses in field populations of aedes aegypti in delhi and haryana states in india-a preliminary report.	aedes aegypti and aedes albopictus are principal vectors for the transmission of chikungunya virus (chikv). india is a hub for both dengue and chikungunya infections and there are several reports of co-infection of dengue and chikungunya virus in the clinical scenario. the present pilot entomological survey was conducted to evaluate vertical transmission of chikv in aedes field populations. aedes immature (larvae and pupae) collection was done in 2012, over a period of six months from selected s ...	2016	27282096
chikungunya virus infection of aedes mosquitoes.	in vivo infection of mosquitoes is an important method to study and characterize arthropod-borne viruses. chikungunya virus (chikv) is a mosquito-borne alphavirus that is transmitted primarily by aedes mosquitoes. in this chapter, we describe a protocol for infection of chikv in two species of aedes mosquitoes, aedes aegypti and aedes albopictus, together with the isolation of chikv in different parts of the infected mosquito such as midgut, legs, wings, salivary gland, head, and saliva. this al ...	2016	27233266
utilization and assessment of throat swab and urine specimens for diagnosis of chikungunya virus infection.	chikungunya is a mosquito-borne infection with clinical presentation of fever, arthralgia, and rash. the etiological agent chikungunya virus (chikv) is generally transmitted from primates to humans through the bites of infected aedes aegypti and aedes albopictus mosquitoes. outbreaks of chikungunya occur commonly with varied morbidity, mortality, and sequele according to the epidemiological, ecological, seasonal, and geographical impact. investigations are required to be conducted as a part of t ...	2016	27233262
reduced incidence of chikungunya virus infection in communities with ongoing aedes aegypti mosquito trap intervention studies - salinas and guayama, puerto rico, november 2015-february 2016.	aedes species mosquitoes transmit chikungunya virus, as well as dengue and zika viruses, and bite most often during the day.* infectious mosquito bites frequently occur in and around homes (1,2). caribbean countries first reported local transmission of chikungunya virus in december 2013, and soon after, chikungunya virus spread throughout the americas (3). puerto rico reported its first laboratory-positive chikungunya case in may 2014 (4), and subsequently identified approximately 29,000 suspect ...	2016	27171600
low seroprevalence indicates vulnerability of eastern and central sudan to infection with chikungunya virus.	outbreaks of infections with chikungunya virus (chikv) have previously been reported from sudan but the prevalence in the general population is unknown. we investigated the seroprevalence of chikv infection in 379 serum samples from patients with fever in the outpatient clinics of three hospitals in eastern and central sudan. the seroprevalence was 1.8%, indicating that chikv infections are rare in these parts of sudan. as the vector aedes aegypti is endemic in this area, the population is at ri ...	2016	26974266
the zika outbreak of the 21st century.	the zika virus outbreak has captivated the attention of the global audience and information has spread rapidly and wildly through the internet and other media channels. this virus was first identified in 1947, when it was isolated from a sentinel rhesus monkey placed by british scientists working at the yellow fever research laboratory located in the zika forest area of uganda, hence its name, and is transmitted primarily by the mosquito vector, aedes aegypti. the fact that the rhesus macaque is ...	2016	26925496
mefenamic acid in combination with ribavirin shows significant effects in reducing chikungunya virus infection in vitro and in vivo.	chikungunya virus (chikv) infection is a persistent problem worldwide due to efficient adaptation of the viral vectors, aedes aegypti and aedes albopictus mosquitoes. therefore, the absence of effective anti-chikv drugs to combat chikungunya outbreaks often leads to a significant impact on public health care. in this study, we investigated the antiviral activity of drugs that are used to alleviate infection symptoms, namely, the non-steroidal anti-inflammatory drugs (nsaids), on the premise that ...	2016	26794398
evidence of vertical transmission and co-circulation of chikungunya and dengue viruses in field populations of aedes aegypti (l.) from guerrero, mexico.	we report results of the entomo-virological surveillance system in aedes aegypti local populations performed by the ministry of health of guerrero.	2016	26711697
[development and basic properties of the viral preparation viroden].	laboratory studies demonstrated the effectiveness of viroden preparation in different doses against preimago stages of aedes aegypti mosquitoes. after treatment of larvae of instar 1 at preimago stages about 77% of the insects died. the preparation is stable on storage and resistant to unfavourable environmental factors: sun radiation, heating, ph variations, without significant loss of activity. the study of the host range of densonucleosis virus, the active principle of the viroden preparation ...	2016	3445591
emergence of zika virus epidemic and the national response in jamaica.	jamaica, along with the americas, experienced major epidemics of arboviral diseases transmitted by the aedes aegypti mosquito in recent years. these include dengue fever in 2012, chikungunya fever in 2014 and zika virus infection (zikv) in 2016. we present the emergence of the zikv epidemic in jamaica and outline the national response.	2016	28375542
inhibition of zika virus by wolbachia in aedes aegypti.	through association with cases of microcephaly in 2015, zika virus (zikv) has transitioned from a relatively unknown mosquito-transmitted pathogen to a global health emergency, emphasizing the need to improve existing mosquito control programs to prevent future disease outbreaks. the response to zika must involve a paradigm shift from traditional to novel methods of mosquito control, and according to the world health organization should incorporate the release of mosquitoes infected with the bac ...	2016	28357366
[zika virus infection in pregnancy, fetal and neonatal impact].	zika virus (zv) is an arbovirus transmitted by aedes aegypti and a. albopictus. the neurotropic profile of this virus is known since 1952. the main finding related to zv in america is microcephaly. two hypotheses are tested on its involvement in the central nervous system: its neurotropic feature and the direct effect of zv on the placenta. malformations and clinical findings on fetal development comprise congenital zika syndrome. rt-pcr and serology (igm) are useful for definitive diagnosis. ho ...	2016	28146192
optomotor reactions reveal polarization sensitvity in the zika virus transmitting yellow fever mosquito aedes (stegomyia) aegypti (diptera; nematocera).	in polarization-sensitive insect species an orthogonal arrangement of photoreceptive microvilli is a characteristic feature. however, mosquito eyes had not revealed this feature, and polarization sensitivity (ps) was considered to be non-existent in them. recently, however, gravid aedes (stegomyia) aegypti females were found to possess ps, sequels of which could be demonstrated only in the absence of chemicals emitted by conspecifics. therefore, ps in ae. aegypti, unlike that of other aquatic in ...	2016	27927093
towards green oviposition deterrents? effectiveness of syzygium lanceolatum (myrtaceae) essential oil against six mosquito vectors and impact on four aquatic biological control agents.	essential oils (eos) from plants may be alternative sources of molecules toxic against mosquito vectors of public health relevance. most of researches in this field focused on eos as larvicides or ovicides, while limited efforts focused on the exploitation of eos as oviposition deterrents. in the present study, the larvicidal and oviposition deterrent activity of syzygium lanceolatum leaf eo was evaluated against six mosquito species, anopheles stephensi, an. subpictus, aedes aegypti, ae. albopi ...	2016	27921244
preparing the united states for zika virus: pre-emptive vector control and personal protection.	discovered in 1947 in a monkey in the zika forest of uganda, zika virus was dismissed as a cause of a mild illness that was confined to africa and southeast asia and transmitted by aedes mosquitoes. in 2007, zika virus appeared outside of its endemic borders in an outbreak on the south pacific island of yap. in 2013, zika virus was associated with a major neurological complication, guillain-barré syndrome, in a larger outbreak in the french polynesian islands. from the south pacific, zika invade ...	2016	28340908
epa-registered repellents for mosquitoes transmitting emerging viral disease.	in many parts of the united states, mosquitoes were previously nuisance pests. however, they now represent a potential threat in the spread of viral diseases. the aedes aegypti, aedes albopictus, and culex species mosquitoes are endemic to the united states and together may transmit a variety of viral diseases of growing concern, including west nile virus, chikungunya, dengue fever, and zika virus. the centers for disease control and prevention and the environmental protection agency (epa) recom ...	2016	27779781
genetic drift during systemic arbovirus infection of mosquito vectors leads to decreased relative fitness during host switching.	the emergence of mosquito-borne rna viruses, such as west nile virus (wnv), is facilitated by genetically complex virus populations within hosts. here, we determine whether wnv enzootic (culex tarsalis, cx. quinquefasciatus, and cx. pipiens) and bridge vectors (aedes aegypti) have differential impacts on viral mutational diversity and fitness. during systemic mosquito infection, wnv faced stochastic reductions in genetic diversity that rapidly was recovered during intra-tissue population expansi ...	2016	27049584
larvicidal activity of magnolia denudata seed hydrodistillate constituents and related compounds and liquid formulations towards two susceptible and two wild mosquito species.	anopheles sinensis, aedes aegypti and aedes albopictus and culex pipiens pallens mosquitoes transmit malaria, dengue fever and west nile virus diseases respectively. this study was conducted to determine the toxicity of 17 constituents from magnolia denudata seed hydrodistillate (md-shd) and four experimental md-shd liquid formulations (10-50 mg l(-1) liquid) to third-instar larvae from insecticide-susceptible cx. p. pallens and ae. aegypti as well as wild ae. albopictus and an. sinensis.	2016	26085316
zika virus in brazil and the danger of infestation by aedes (stegomyia) mosquitoes.	zika virus, already widely distributed in africa and asia, was recently reported in two northeastern brazilian: state of bahia and state of rio grande do norte, and one southeastern: state of são paulo. this finding adds a potentially noxious virus to a list of several other viruses that are widely transmitted by aedes (stegomyia) aegypti and aedes (stegomyia) albopictus in brazil. the pathology and epidemiology, including the distribution and vectors associated with zika virus, are reviewed. th ...	2016	26689277
zika control through the bacterium wolbachia pipientis.		2016	27831765
development of a sybr green i-based quantitative rt-pcr for ross river virus: application in vector competence studies and antiviral drug evaluation.	ross river virus (rrv) is an emerging alphavirus and is presently endemic in many parts of oceania. keeping in mind its emergence, we developed a molecular detection system and utilized it to study vector competence and evaluate activity of antiviral compounds against rrv. a sybr green i-based quantitative rt-pcr for detection of rrv was developed targeting the e2 gene, with a detection limit of 100 rna copies/reaction. the specificity was confirmed with closely related alphaviruses and flavivir ...	2016	27105737
chemical composition, antimicrobial and insecticidal activities of flowers essential oils of alpinia zerumbet (pers.) b.l.burtt & r.m.sm. from martinique island.	alpinia species, used as ornamental plants and flavoring agents, are renowned for their therapeutic properties and their subsequent use in traditional medicines throughout the world. alpinia zerumbet (pers.) b.l.burtt & r.m.sm. is the most common alpinia species encountered in martinique. several essential oils (eos) obtained by hydrodistillation of a. zerumbet flowers collected in various locations on the island at different seasons were analysed to evaluate the influence of the collection peri ...	2016	27935668
mycobacterium ulcerans toxin, mycolactone may enhance host-seeking and oviposition behaviour by aedes aegypti (l.) (diptera: culicidae).	the ecological functions of many toxins continue to remain unknown for those produced by environmental pathogens. mycobacterium ulcerans, the causative agent of the neglected tropical disease, buruli ulcer, produces a cytotoxic macrolide, mycolactone, whose function(s) in the environment remains elusive. through a series of dual-choice behaviour assays, they show that mycolactone may be an interkingdom cue for the yellow fever mosquito, aedes aegypti, seeking blood-meals as well as oviposition s ...	2016	27907248
assessment of the ability of aedes species mosquitoes to transmit feline mycoplasma haemofelis and 'candidatus mycoplasma haemominutum'.	the objective of this study was to evaluate wild-caught mosquitoes for evidence of hemotropic mycoplasma species dna and to determine whether the feline hemoplasmas, mycoplasma haemofelis (mhf) and 'candidatus mycoplasma haemominutum' (mhm), can be transmitted by aedes aegypti mosquitoes in a laboratory setting.	2016	27418288
declining malaria, rising of dengue and zika virus: insights for mosquito vector control.	the fight against mosquito-borne diseases is a challenge of huge public health importance. to our mind, 2015 was an extraordinary year for malaria control, due to three hot news: the nobel prize to youyou tu for the discovery of artemisinin, the development of the first vaccine against plasmodium falciparum malaria [i.e. rts,s/as01 (rts,s)], and the fall of malaria infection rates worldwide, with special reference to sub-saharan africa. however, there are major challenges that still deserve atte ...	2016	26932263
uv-b radiation reduces in vitro germination of metarhizium anisopliae s.l. but does not affect virulence in fungus-treated aedes aegypti adults and development on dead mosquitoes.	control of diurnal aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to uv-b radiation that might affect activity of conidia applied on the mosquito's surface.	2016	27685030
determination and characterization of destruxin production in metarhizium anisopliae tk6 and formulations for aedes aegypti mosquitoes control at the field level.	destruxins, cyclic hexadepsipeptide toxins, secreted by the entomopathogenic fungus, metarhizium anisopliae through extracellular synthesis. the present study reports a new approach for the analysis of dtxs produced by the fungal strain metarhizium anisoliae tk6, using frir-hplc-lc-ms and h(1) nmr. the results also showed that production of the major dtxs a, b, c, and e have to be determined in czapek dextrose (cd) liquid culture filtrate from 9 to 12 days post-inoculation. purified dtx were fur ...	2016	27452930
raw sewage as breeding site to aedes (stegomyia) aegypti (diptera, culicidae).	the selection of oviposition sites by females of aedes (stegomyia) aegypti is a key factor for the larval survival and egg dispersion and has a direct influence in vector control programs. in this study, we evaluated the aspects of reproductive physiology of ae. aegypti mosquitoes tested in the presence of raw sewage. ae. aegypti females were used in oviposition bioassays according to two methodologies: (i) choice assay, in which three oviposition substrates were offered in the same cage: treatm ...	2016	27640323
identification of aadnr1, a novel gene related to innate immunity and apoptosis in aedes albopictus.	innate immunity and apoptosis play critical roles in defending pathogens in insects. in drosophila, dnr1 was reported as a negative regulator of apoptosis and immune deficiency (imd) pathway which belongs to innate immunity. aedes albopictus is an important kind of arbovirus vector and becoming a significant threat to public health due to its rapid global expansion. here we identified an ortholog of dnr1 from a. albopictus, named as aadnr1. aadnr1 encoded a putative protein containing an n-termi ...	2016	27045774
easy and inexpensive molecular detection of dengue, chikungunya and zika viruses in febrile patients.	dengue (denv), chikungunya (chikv) and zika (zikv) are arthropod-borne viruses (arboviruses) sharing a common vector, the mosquito aedes aegypti. at initial stages, patients infected with these viruses have similar clinical manifestations, however, the outcomes and clinical management of these diseases are different, for this reason early and accurate identification of the causative virus is necessary. this paper reports the development of a rapid and specific nested-pcr for detection of denv, c ...	2016	27477452
comparative susceptibility of ochlerotatus japonicus, ochlerotatus triseriatus, aedes albopictus, and aedes aegypti (diptera: culicidae) to la crosse virus.	invasive mosquito species can increase the transmission risk of native mosquito-borne diseases by acting as novel vectors. in this study, we examined the susceptibility of three exotic invasive mosquito species aedes aegypti (l.), ae. albopictus (skuse), and ochlerotatus japonicus (theobald) to la crosse virus (lacv) relative to the native primary vector ochlerotatus triseriatus (say). adult females of the four mosquito species were orally challenged with lacv; incubated for 3, 5, 7, 9, or 11 d; ...	2016	27605372
antimicrobial and antileishmanial activities of diterpenoids isolated from the roots of salvia deserta.	four diterpenes with biological activity were isolated from salvia deserta roots. taxodione was considered leishmanicidal with an ic50 value of 1.46 µm (0.46 mg/l) against leishmania donovani and also exhibited antifungal and antimicrobial activities. ferruginol displayed the greatest activity [24-h ic50 of 4.5 µm (1.29 mg/l)] against the fish pathogenic bacteria streptococcus iniae. the crude extract fraction that contained the isolated compounds 7-o-acetylhorminone and horminone showed stronge ...	2016	26308356
characterization of a fasciola gigantica protein carrying two dm9 domains reveals cellular relocalization property.	even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. classified among the proteins of unknown function is a family of proteins harboring repeats of the dm9 domain, a 60-75 amino acids motif first described in a small number of drosophila melanogaster proteins. proteins may carry two or more dm9 domains either in combination with other domains or as their sole constituent. here we have charact ...	2016	26946400
larvicidal and adulticidal activity of chroman and chromene analogues against susceptible and permethrin-resistant mosquito strains.	mosquitoes play a major role as vectors that transmit parasitic and viral diseases worldwide, especially in tropical and subtropical countries. mosquito borne diseases not only affect humans but they also affect livestock in many parts of the world. they carry diseases that are lethal to dogs and horses. dog heartworm disease (dirofilaria immitis) is a parasitic disease spread through mosquitoes. this disease is not limited to dogs, but it can affect other animals and humans as well. eastern equ ...	2016	27249182
why zika virus infection has become a public health concern?	prior to 2015, zika virus (zikv) outbreaks had occurred in areas of africa, southeast asia, and the pacific islands. although a causal relationship between zika infection during pregnancy and microcephaly is strongly suspected, such a connection has not yet been scientifically proven. in may 2015, the outbreak of zikv infection in brazil led to reports of syndrome and pregnant women giving birth to babies with birth defects and poor pregnancy outcomes; the pan american health organization (paho) ...	2016	27052792
management and treatment of dengue and chikungunya - natural products to the rescue.	neglected tropical diseases (ntds) flourish mostly in impoverished developing nations of the world. it is estimated that ntds plague up to 1 billion people every year thereby inducing a massive economic and health burden worldwide. following explosive outbreaks mostly in asia, latin america, europe and the indian ocean, two common ntds namely, chikungunya and dengue both transmitted by an infected mosquito vector principally aedes aegypti have emerged as a major public health threat. given the l ...	2016	27151484
molecular characterization of two major dengue outbreaks in costa rica.	dengue virus (denv) (flavivirus, flaviviridae) is a reemerging arthropod-borne virus with a worldwide circulation, transmitted mainly by aedes aegypti and aedes albopictus mosquitoes. since the first detection of its main transmitting vector in 1992 and the invasion of denv-1 in 1993, costa rica has faced dengue outbreaks yearly. in 2007 and 2013, costa rica experienced two of the largest outbreaks in terms of total and severe cases. to provide genetic information about the etiologic agents prod ...	2016	27139442
dengue virus immunopathogenesis: lessons applicable to the emergence of zika virus.	dengue is the leading mosquito-transmitted viral infection in the world. there are more than 390 million new infections annually; while the majority of infected individuals are asymptomatic or develop a self-limited dengue fever, up to 1 million clinical cases develop severe manifestations, including dengue hemorrhagic fever and shock syndrome, resulting in ~25,000 deaths annually, mainly in children. gaps in our understanding of the mechanisms that contribute to dengue infection and immunopatho ...	2016	27130436
production of dengue virus envelope protein domain iii-based antigens in tobacco chloroplasts using inducible and constitutive expression systems.	dengue fever is a disease in many parts of the tropics and subtropics and about half the world's population is at risk of infection according to the world health organization. dengue is caused by any of the four related dengue virus serotypes den-1, -2, -3 and -4, which are transmitted to people by aedes aegypti mosquitoes. currently there is only one vaccine (dengvaxia(®)) available (limited to a few countries) on the market since 2015 after half a century's intensive efforts. affordable and ac ...	2016	27116001
effects of larval nutrition on wolbachia-based dengue virus interference in aedes aegypti (diptera: culicidae).	in order to assess the broad-scale applicability of field releases of wolbachia for the biological control of insect-transmitted diseases, we determined the relationship between the larval diet of aedes aegypti l. mosquitoes infected with wolbachia strains and their susceptibility to dengue virus (denv) infection via intrathoracic injection and oral inoculation. larvae were reared on diets that varied in the quantity of food which had the effect of modifying development time and adult body size. ...	2016	27106932
lethal dengue virus infection: a forensic overview.	dengue virus is a single-stranded rna virus that is a member of the family flaviviridae, genus flavivirus. it is usually transmitted by the female aedes aegypti mosquito. dengue fever is a febrile illness caused by 1 of 4 serotypes of the virus, which may progress to dengue hemorrhagic fever or dengue shock syndrome. the mortality rate of untreated dengue shock syndrome is more than 20%. the reported incidence has increased 30-fold for the past 50 years with an estimated 50 to 100 million dengue ...	2016	27093563
vector surveillance for dengue virus detection in the archipelago of fernando de noronha, brazil.	aedes aegypti (l.) has become an efficient vector of important arboviruses due to its anthropophilic and domiciliary behaviors. since the 1980s, dengue affects thousands of people every year in brazil; in fernando de noronha (fn), a touristic archipelago, dengue cases have occurred since 2001. once ae. aegypti populations are well established in the inhabited areas of fn, the threat of dengue or another arbovirus epidemic is continuously imminent. this study aimed to monitor the denv serotypes i ...	2016	27067800
mosquito vectors and the spread of cancer: an overlooked connection?	mosquitoes (diptera: culicidae) represent a key threat for millions of humans and animals worldwide, vectoring important pathogens and parasites, including malaria, dengue, filariasis, and zika virus. besides mosquito-borne diseases, cancers figure among the leading causes of mortality worldwide. it is expected that annual cancer cases will rise from 14 million in 2012 to 22 million within the next two decades. notably, there are few contrasting evidences of the relationship between cancer and m ...	2016	27053131
dengue virus-1 infection did not alter the behavioral response of aedes aegypti (diptera: culicidae) to deet.	no licensed vaccine or antiviral drug against dengue virus (denv) is available; therefore, most of the effort to prevent this disease is focused on reducing vector-host interactions. one of the most widely accepted methods of blocking vector-human contact is to use insect repellents to interfere with mosquito host-seeking behavior. some arboviruses can replicate in the nervous system of the vector, raising the concern that arboviral infection may alter the insect behavioral response toward chemi ...	2016	27026163
notes from the field: evidence of zika virus infection in brain and placental tissues from two congenitally infected newborns and two fetal losses--brazil, 2015.	zika virus is a mosquito-borne flavivirus that is related to dengue virus and transmitted primarily by aedes aegypti mosquitoes, with humans acting as the principal amplifying host during outbreaks. zika virus was first reported in brazil in may 2015 (1). by february 9, 2016, local transmission of infection had been reported in 26 countries or territories in the americas.* infection is usually asymptomatic, and, when symptoms are present, typically results in mild and self-limited illness with s ...	2016	26890059
the effect of temperature on wolbachia-mediated dengue virus blocking in aedes aegypti.	dengue fever, caused by dengue virus (denv), is endemic in more than 100 countries. the lack of effective treatment of patients and the suboptimal efficacies of the tetravalent vaccine in trials highlight the urgent need to develop alternative strategies to lessen the burden of dengue fever.wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits the replication of the denv in the mosquito vector,aedes aegypti however, ...	2016	26856916
aedes aegypti anti-salivary gland antibody concentration and dengue virus exposure history in healthy individuals living in an endemic area in colombia.	mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. igg antibodies against dengue virus (denv-igg) are indicators of previous exposure. the risk of denv transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology.	2016	26844447
human c5a protein participates in the mosquito immune response against dengue virus.	dengue virus (denv) is transmitted by aedes spp mosquitoes during a bloodmeal uptake. the bloodmeal consists of host cells, immune factors, and possibly blood-borne pathogens, such as arboviruses. human cells and immune-related factors, like the complement system, can remain active in the bloodmeal and may be able to interact with pathogens in the mosquito. previous studies have shown that active complement proteins impact plasmodium parasite viability in the anopheles midgut. thus, we investiga ...	2016	26843451
infection of mosquito cells (c6/36) by dengue-2 virus interferes with subsequent infection by yellow fever virus.	dengue is one of the most important diseases caused by arboviruses in the world. yellow fever is another arthropod-borne disease of great importance to public health that is endemic to tropical regions of africa and the americas. both yellow fever and dengue viruses are flaviviruses transmitted by aedes aegypti mosquitoes, and then, it is reasonable to consider that in a given moment, mosquito cells could be coinfected by both viruses. therefore, we decided to evaluate if sequential infections o ...	2016	26808727
natural transmission of dengue virus serotype 3 by aedes albopictus (skuse) during an outbreak in havelock island: entomological characteristics.	from may to june 2014, an outbreak of dengue virus (denv) illness occurred in the havelock island, south andaman. entomological investigations were undertaken during the peak of the outbreak, from 26th may-4th june, to identify the primary vector(s) involved in the transmission so that appropriate public health measures could be implemented. adult mosquitoes were collected by bg-sentinel traps in houses and neighborhoods of clinically ill patients. water holding containers were inspected for the ...	2016	26780552
autophagy and viral diseases transmitted by aedes aegypti and aedes albopictus.	despite a long battle that was started by oswaldo cruz more than a century ago, in 1903, brazil still struggles to fight aedes aegypti and aedes albopictus, the mosquito vectors of dengue virus (denv), chikungynya virus (chikv) and zika virus (zikv). dengue fever has been a serious public health problem in brazil for decades, with recurrent epidemic outbreaks occurring during summers. in 2015, until november, 1,534,932 possible cases were reported to the ministry of healthv. more recently, the l ...	2016	26774331
a native wolbachia endosymbiont does not limit dengue virus infection in the mosquito aedes notoscriptus (diptera: culicidae).	the endosymbiotic bacterium wolbachia pipientis infects many species of insects and has been transinfected into the mosquito aedes aegypti (l.), the primary vector of dengue virus (denv). recently, it has been shown that wolbachia blocks the replication and transmission of rna viruses, such as denv, in a number of mosquito species including ae. aegypti and aedes albopictus (skuse), which is naturally infected with wolbachia and considered a secondary vector for denv. the mosquito species aedes n ...	2016	26721865
clinical and epidemiological characterization of the first recognized outbreak of dengue virus-type 2 in mozambique, 2014.	since the first reported epidemic of dengue in pemba, the capital of cabo delgado province, in 1984-1985, no further cases have been reported in mozambique. in march 2014, the provincial health directorate of cabo delgado reported a suspected dengue outbreak in pemba, associated with a recent increase in the frequency of patients with nonmalarial febrile illness. an investigation conducted between march and june detected a total of 193 clinically suspected dengue patients in pemba and nampula, t ...	2016	26643534
fighting arboviral diseases: low toxicity on mammalian cells, dengue growth inhibition (in vitro), and mosquitocidal activity of centroceras clavulatum-synthesized silver nanoparticles.	dengue is a mosquito-borne viral disease that has rapidly spread in all regions of the world in recent years. female mosquitoes, mainly aedes aegypti, transmit dengue. approximately 3,900 million people, in 128 countries, are at risk of dengue infection. recently, a focus has been provided on the potential of green-synthesized nanoparticles as inhibitors of the production of dengue viral envelope (e) protein in vero cells and downregulators of the expression of dengue viral e gene. algae are an ...	2016	26462804
bioactivities of a new pyrrolidine alkaloid from the root barks of orixa japonica.	a new pyrrolidine alkaloid named (z)-3-(4-hydroxybenzylidene)-4-(4-hydroxyphenyl)-1-methylpyrrolidin-2-one was isolated from the ethanol extract of the root barks of orixa japonica. the structure of the new alkaloid was elucidated on the basis of nmr and ms analysis. the compound exhibited larvicidal activity against the fourth instar larvae of aedes aegypti (lc50 = 232.09 μg/ml), anopheles sinensis (lc50 = 49.91 μg/ml), and culex pipiens pallens (lc50 = 161.10 μg/ml). the new alkaloid also poss ...	2016	27918451
cloning and expressing trypsin modulating oostatic factor in chlorella desiccata to control mosquito larvae.	the insect peptide hormone trypsin modulating oostatic factor (tmof), a decapeptide that is synthesized by the mosquito ovary and controls the translation of the gut's trypsin mrna was cloned and expressed in the marine alga chlorella desiccata. to express aedes aegypti tmof gene (tmfa) in c. desiccata cells, two plasmids (pyes2/tmof and pydb4-tmfa) were engineered with pkylx71 dna (5 kb) carrying the cauliflower mosaic virus (camv) promoter 35s(2) and the kanamycin resistant gene (neo), as well ...	2016	26440910
synthesis and biological evaluation of 3,5-dimethoxystilbene analogs.	in our continuing effort to discover natural product-based pest management agents, derivatives of 3,5-dimethoxystilbene were synthesized yielding 27 new and six known compounds. compounds 11 and 12 showed strong aedes aegypti larvicidal activity (lc50 45.31 and 49.93 μm, respectively). furthermore, 11 and 12 exhibited high effectiveness against larvae of pesticide-susceptible and pyrethroid-resistant strains of ae. aegypti; activity against the adult mosquitoes was low. compounds 6f, 6g, and 6i ...	2016	27450661
[reasons to recommend vaccination against dengue in easter island: immunization advisory committee of sociedad chilena de infectología].	dengue was first diagnosed on easter island on year 2002 and thereafter recurrent outbreaks have occurred involving different serotypes of dengue virus. its vector, aedes aegypti has not been eliminated despite the small size of the island. conditions at the local hospital preclude adequate management of severe and hemorrhagic cases due to the absence of a critical care unit as well as no availability of platelets, or plasma units for transfusion. besides, transfer, of severely affected patients ...	2016	27905629
rise and fall of vector infectivity during sequential strain displacements by mosquito-borne dengue virus.	each of the four serotypes of mosquito-borne dengue virus (denv-1-4) comprises multiple, genetically distinct strains. competitive displacement between strains within a serotype is a common feature of denv epidemiology and can trigger outbreaks of dengue disease. we investigated the mechanisms underlying two sequential displacements by denv-3 strains in sri lanka that each coincided with abrupt increases in dengue haemorrhagic fever (dhf) incidence. first, the post-dhf strain displaced the pre-d ...	2016	27500505
secreted ns1 protects dengue virus from mannose-binding lectin-mediated neutralization.	flavivirus nonstructural protein 1 (ns1) is a unique secreted nonstructural glycoprotein. although it is absent from the flavivirus virion, intracellular and extracellular forms of ns1 have essential roles in viral replication and the pathogenesis of infection. the fate of ns1 in insect cells has been more controversial, with some reports suggesting it is exclusively cell associated. in this study, we confirm ns1 secretion from cells of insect origin and characterize its physical, biochemical, a ...	2016	27798151
evolutionary potential of the extrinsic incubation period of dengue virus in aedes aegypti.	dengue fever is the most common arboviral disease worldwide. it is caused by dengue viruses (denv) and the mosquito aedes aegypti is its primary vector. one of the most powerful determinants of a mosquito's ability to transmit denv is the length of the extrinsic incubation period (eip), the time it takes for a virus to be transmitted by a mosquito after consuming an infected blood meal. here, we repeatedly measured denv load in the saliva of individual mosquitoes over their lifetime and used thi ...	2016	27530960
non-human primate antibody response to mosquito salivary proteins: implications for dengue virus transmission in puerto rico.	an important step to incriminate a mosquito as a vector of a disease pathogen is finding evidence of direct contact between the mosquito and humans. typically, this is accomplished through landing/biting catches, or host blood meal analysis in engorged mosquitoes via immunologic assays. an alternate approach is to identify the presence of specific mosquito anti-saliva protein antibodies in the blood of exposed hosts. following the discovery of dengue infected, free roaming non-human primates in ...	2016	27593498
dengue: an analysis of epidemiological pattern over a six year period.	dengue is an arboviral infection transmitted among humans by aedes aegypti, the urban vector and aedes albopictus as a maintenance vector in sub-urban and rural areas. the diagnosis is routinely established by detection of ns1 antigen and/or by igm antibodies testing. dengue infects 50-100 million cases per year worldwide with atleast half million serious cases needing hospitalization. therefore, it is necessary to keep the surveillance ongoing to timely look out for changes occurring in disease ...	2016	28208855
costs and benefits of vertical and horizontal transmission of dengue virus.	parasites can be transmitted vertically and/or horizontally, but the costs or benefits for the host of infection have only been tested after horizontal transmission. here, we report for the first time, to our knowledge, the survival, reproduction and infection of aedes aegypti during vertical and horizontal transmission of dengue virus 2 (denv-2). females infected horizontally produced more eggs, with a sex ratio skewed towards males, compared with uninfected controls. however, there was no sign ...	2016	27618859
aedes aegypti (diptera: culicidae) longevity and differential emergence of dengue fever in two cities in sonora, mexico.	dengue virus, primarily transmitted by the aedes aegypti (l.) mosquito, has rapidly expanded in geographic extent over the past several decades. in some areas, however, dengue fever has not emerged despite established ae. aegypti populations. the reasons for this are unclear and have sometimes been attributed to socio-economic differences. in 2013 we compared ae. aegypti adult density and population age structure between two cities in sonora, mexico: hermosillo, which has regular seasonal dengue ...	2016	27590231
dengue infection.	dengue is widespread throughout the tropics and local spatial variation in dengue virus transmission is strongly influenced by rainfall, temperature, urbanization and distribution of the principal mosquito vector aedes aegypti. currently, endemic dengue virus transmission is reported in the eastern mediterranean, american, south-east asian, western pacific and african regions, whereas sporadic local transmission has been reported in europe and the united states as the result of virus introductio ...	2016	27534439
mosquito avoidance practices and knowledge of arboviral diseases in cities with differing recent history of disease.	as the range of dengue virus (denv) transmission expands, an understanding of community uptake of prevention and control strategies is needed both in geographic areas where the virus has recently been circulating and in areas with the potential for denv introduction. personal protective behaviors such as the use of mosquito repellent to limit human-vector contact and the reduction of vector density through elimination of oviposition sites are the primary control methods for aedes aegypti, the ma ...	2016	27527634
update: ongoing zika virus transmission - puerto rico, november 1, 2015-july 7, 2016.	zika virus is a flavivirus transmitted primarily by aedes aegypti and aedes albopictus mosquitoes, and infection can be asymptomatic or result in an acute febrile illness with rash (1). zika virus infection during pregnancy is a cause of microcephaly and other severe birth defects (2). infection has also been associated with guillain-barré syndrome (gbs) (3) and severe thrombocytopenia (4,5). in december 2015, the puerto rico department of health (prdh) reported the first locally acquired case o ...	2016	27490087
zika virus: a new arboviral public health problem.	zika virus (zikv) is a single-stranded rna virus in the flaviviridae family and transmitted to human through infected mosquitos (aedes aegypti and aedes albopictus). virus is closely related with other flaviviruses; dengue virus, yellow fever virus, west nile virus, and japanese encephalitis virus phylogenetically. due to the possible relationship between virus and clinical features including microcephaly, ventricule, and eye deformities, guillain-barre syndrome increases the interest on this vi ...	2016	27469112
apoptosis-related genes control autophagy and influence denv-2 infection in the mosquito vector, aedes aegypti.	the mosquito aedes aegypti is the primary urban vector for dengue virus (denv) worldwide. insight into interactions occurring between host and pathogen is important in understanding what factors contribute to vector competence. however, many of the molecular mechanisms for vector competence remain unknown. our previous global transcriptional analysis suggested that differential expression of apoptotic proteins is involved in determining refractoriness vs susceptibility to denv-2 infection in ae. ...	2016	27418459
zika virus infections: an overview of current scenario.	zika virus (zikv) was discovered more than half a century ago, recently it has gained unprecedented attention by the global health community. until 2007, only 14 cases of human zikv infections were reported around the globe, while during the current outbreak, estimated cases mounted to approximately 1.5 million in brazil alone, the virus was disseminated to wider south-american territories and travel-associated zikv infections were reported in usa, europe and recently in china. zikv infections r ...	2016	27393087
dengue protease substrate recognition: binding of the prime side.	dengue virus (denv), transmitted predominantly in tropical and subtropical regions by the mosquito aedes aegypti, infects millions of people and leads to dengue fever and thousands of deaths each year. there are no direct-acting antivirals to combat denv, and molecular and structural knowledge is required to develop such compounds. the dengue ns2b/ns3 protease is a promising target for direct-acting antivirals, as viral polyprotein cleavage during replication is required for the maturation of th ...	2016	27657335
the mysterious zika virus: adding to the tropical flavivirus mayhem.	until now, known as the demure cousin of dengue virus (denv) inhabiting africa, zika virus (zikv) has reinvented itself to cause explosive epidemics captivating the western hemisphere. the outbreak causing potential for zikv was realized when it made its way from africa to yap island micronesia in 2007, and in french polynesia in 2013. from there, it moved on to brazil in 2015. now zikv has infected people in more than 33 countries in central and south america and the caribbean. moreover the epi ...	2016	27652985
presence of aedes (stegomyia) aegypti (linnaeus, 1762) and its natural infection with dengue virus at unrecorded heights in colombia.	aedes aegypti is the main vector of urban yellow fever, dengue, chikungunya and zika viruses. the biogeographical distribution of this species has expanded due to global warming, and socioeconomic and cultural factors. the changes in the altitudinal distribution patterns of this vector and its natural infection are priority fields of research to develop entomological, virological and public health surveillance strategies.	2016	27622492
wolbachia: the selfish trojan horse in dengue control.	dengue fever has re-emerged as a major public health challenge. of late, several promising attempts have been made to control the disease with limited success. an innovative method of biological control of dengue is the use of the bacterium wolbachia. selected strains of wolbachia have been introduced into aedes aegypti to prevent transmission of dengue viruses by the vector. wolbachia prevents dengue transmission by either directly blocking the virus or by decreasing the lifespan of the vector. ...	2016	27843186
vector competence of aedes albopictus and aedes aegypti (diptera: culicidae) for the den2-fj10 and den2-fj11 strains of the dengue 2 virus in fujian, china.	dengue is an acute, emerging, infectious disease transmitted by aedes mosquitoes that has become a serious global public health problem. the den2-fj10 and den2-fj11 strains of the dengue 2 virus were originally isolated from the serum of a patient with dengue fever in fujian province, china, in 1999. our data provide the first assessment of the vector competence of aedes mosquitoes with respect to the den2-fj10 and den2-fj11 strains of the dengue virus. there were significant differences in the ...	2016	27260668
a novel mosquito ubiquitin targets viral envelope protein for degradation and reduces virion production during dengue virus infection.	dengue virus (denv) is a mosquito-borne flavivirus that causes significant human disease and mortality in the tropics and subtropics. by examining the effects of virus infection on gene expression, and interactions between virus and vector, new targets for prevention of infection and novel treatments may be identified in mosquitoes. we previously performed a microarray analysis of the aedes aegypti transcriptome during infection with denv and found that mosquito ubiquitin protein ub3881 (aael003 ...	2016	27241849
dengue deaths: associated factors and length of hospital stay.	background. dengue continues to pose a public health problem globally. objective. to review factors associated with patients who died from dengue in trinidad. methods. a retrospective case note review of hospitalized patients who died during 2001 to 2010. results. a total of 23 cases were identified: 13 males, 10 females-12 east indians, 9 africans, and 2 unknown. more than half (n = 17) were over 40 years of age with 10 being over 60 years of age; three were children. a falling platelet count w ...	2016	27462472
tourniquet test for dengue diagnosis: systematic review and meta-analysis of diagnostic test accuracy.	dengue fever is a ubiquitous arboviral infection in tropical and sub-tropical regions, whose incidence has increased over recent decades. in the absence of a rapid point of care test, the clinical diagnosis of dengue is complex. the world health organisation has outlined diagnostic criteria for making the diagnosis of dengue infection, which includes the use of the tourniquet test (tt).	2016	27486661
afrocolombian ethnicity, a paradoxical protective factor against dengue.	dengue is a priority public health problem. during epidemics in cuba and haiti, ethnic african descendant population had lower risk of dengue, and the ethnic factor was proposed as a protective one.	2016	27821892