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effect of bacterial cell-free supernatants on infectivity of norovirus surrogates.bacterial metabolic products were evaluated for inhibitory effects on viral propagation in cell culture. cell-free supernatants (cfs) were prepared from growth of enterococcus faecalis atcc 19433, pseudomonas fluorescens atcc 13525, escherichia coli 08, staphylococcus epidermidis atcc 12228, bacillus subtilis 168, bacillus coagulans 185a, b. coagulans 7050, clostridium sporogenes pa3679, and a commercial probiotic mixture of lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium bif ...201424406013
antibacterial, anti-inflammatory and probiotic potential of enterococcus hirae isolated from the rumen of bos primigenius.in the present study bacterial strains were isolated from the rumen fluids of bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens vibrio cholera, enterococcus faecalis, enterobacter aerogens, pseudomonas ...201424609495
bacterial community migration in the ripening of doenjang, a traditional korean fermented soybean food.doenjang, a traditional korean fermented soybean paste, is made by mixing and ripening meju with high salt brine (approximately 18%). meju is a naturally fermented soybean block prepared by soaking, steaming, and molding soybean. to understand living bacterial community migration and the roles of bacteria in the manufacturing process of doenjang, the diversity of culturable bacteria in meju and doenjang was examined using media supplemented with nacl, and some physiological activities of predomi ...201424548930
the disinfecting efficacy of root canals with laser photodynamic therapy.infecting microorganisms of the root canals are difficult to eliminate during endodontic treatment. in this study the effect of root canal disinfection with photodynamic therapy (pdt) at different time intervals in comparison to 2.5% sodium hypochlorite (naocl) irrigation and passive ultrasonic irrigation (pui) in extracted teeth colonized with enterococcus faecalis and candida albicans was tested to assess which treatment reaches the best disinfection rate.201425606335
antimicrobial activity and stability of electron beam irradiated dental irrigants.the electron beam (e-beam) radiation is considered as an effective means of sterilization of healthcare products as well as to induce the structural changes in the pharmaceutical agents/drug molecules. in addition to structural changes of pharmaceutical it also induces the formation of low molecular weight compounds with altered microbiological, physicochemical and toxicological properties. among the several known medicaments, sodium hypochlorite (naocl) and chlorhexidine digluconate (chx) are u ...201425584220
antimicrobial and immunomodulatory properties of pgla-am1, cpf-am1, and magainin-am1: potent activity against oral pathogens.cationic amphipathic α-helical peptides are intensively studied classes of host defence peptides (hdps). three peptides, peptide glycine-leucine-amide (pgla-am1), caerulein-precursor fragment (cpf-am1) and magainin-am1, originally isolated from norepinephrine-stimulated skin secretions of the african volcano frog xenopus amieti (pipidae), were studied for their antimicrobial and immunomodulatory activities against oral and respiratory pathogens. minimal effective concentrations (mecs), determine ...201425447193
microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. in vitro sem study.this in vitro study aimed to investigate the ability of candida albicans (c. albicans) and enterococcus faecalis (e. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth.201425383343
antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review - part i. in vitro studies.the goal of endodontic treatment is the prevention and control of pulpal and periradicular infections. calcium hydroxide (ca(oh)2) has been widely used in endodontics as an intracanal medicament to eliminate the remaining microorganisms after chemomechanical preparation. the purpose of this article is to review the antimicrobial properties of ca(oh)2 as an intracanal medicament in root canal treatment. the first part of this review details the characteristics of ca(oh)2 and summarizes the result ...201425383341
in vitro evaluation of the antimicrobial efficacy of four endodontic biomaterials against enterococcus faecalis, candida albicans, and staphylococcus aureus.root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. this study evaluated the antimicrobial activity of four endodontic sealers, ah plus, tubliseal ewt, endorez, and iroot sp, against three different microorganisms, e. faecalis, c. albicans, and s. aureus, by direct contact test. 10 μl microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°c. subsequentl ...201425371678
[clinical and bacteriological characteristic of infectious purulent complications in patients after related renal transplantation].we investigated the frequency and characteristics of infectious purulent and non-infectious complications in living related renal transplant recipients in early postoperative period. it was identified the prevalent microorganisms in urinary tract infections and its antibiotic sensitivity: pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, citrobacter freundii, staphylococcus epidermidis, enterococcus faecalis, candida albicans. 182 strains of bacteria and cand ...201425327674
comparative evaluation of antimicrobial activity of two root canal sealers: mta fillapex and ah 26.even after a complete root canal therapy, reinfection may occur as a result of incomplete seal and activation of residual bacteria. thus, antimicrobial activity is an important characteristic of root canal sealers. mta fillapex is a newly developed mta-based sealer. it has been subjected to various studies evaluating its physical and chemical properties. in this study, we aimed to compare the antimicrobial activity of this sealer to the commonly known widely accepted sealer, ah26.201425299361
chemical constituent and antimicrobial effect of essential oil from myrtus communis leaves on microorganisms involved in persistent endodontic infection compared to two common endodontic irrigants: an in vitro study.persistent infections of human root canals play a fundamental role in the failure of endodontic treatment. the purpose of this study is to determine the chemical composition of myrtus communis (m. communis) essential oil and to assess its antimicrobial activity against enterococcus faecalis, staphylococcus aureus and candida albicans compared to that of sodium hypochlorite (naocl) and chlorhexidine (chx).201425298646
in vitro antimicrobial activity of an experimental dentifrice based on ricinus communis.this study evaluated the antimicrobial activity of a ricinus communis-based experimental dentifrice for denture hygiene against the following standard strains: staphylococcus aureus, escherichia coli, streptococcus mutans, enterococcus faecalis, candida albicans and candida glabrata. the minimum inhibitory concentration (mic) assay was performed with r. communis in pure oil at 2.5%. only e. coli was not inhibited by r. communis, but the mic (0.0781%) was effective against the other microorganism ...201425252252
which tree orders in southern africa have the highest antimicrobial activity and selectivity against bacterial and fungal pathogens of animals?the study randomly screened leaf extracts of several hundred southern african tree species against important microbial pathogens to determine which taxa have the highest activity and may yield useful products to treat infections in the animal health market.201425164197
comparative evaluation of antimicrobial efficacy of triple antibiotic paste and calcium hydroxide using chitosan as carrier against candida albicans and enterococcus faecalis: an in vitro study.to analyze the sustained release of intracanal medicaments with or without a carrier and testing their antimicrobial efficacy in root canal against candida albicans and enterococcus faecalis.201425125845
a comparative antimicrobial analysis of various root canal irrigating solutions on endodontic pathogens: an in vitro study.evolution in understanding the poly-microbial environment of both endodontic infections and that of failed root canal treatments has been debatable over the years. the present study was designed to compare and analyze the effect of various root canal irrigation solutions on certain endodontic pathogens in vitro.201425095835
screening and characterisation of antimicrobial properties of semisynthetic betulin derivatives.betulin (lup-20(29)-ene-3β, 28-diol) is a naturally occurring triterpene, which is found in substantial amounts from the outer bark of birch trees. a library of 51 structurally diverse semisynthetic betulin derivatives was screened against five bacterial strains, enterobacter aerogenes, escherichia coli, enterococcus faecalis, pseudomonas aeruginosa, staphylococcus aureus and a fungal strain candida albicans, using broth microdilution assays. primary antimicrobial screening at 50 µm concentratio ...201425032708
the antimicrobial effects of citrus limonum and citrus aurantium essential oils on multi-species biofilms.the aim of this study was to evaluate the effects of citrus limonum and citrus aurantium essential oils (eos) compared to 0.2% chlorhexidine (chx) and 1% sodium hypochlorite (naocl) on multispecies biofilms formed by candida albicans, enterococcus faecalis and escherichia coli. the biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/ml) and incubated at 37°c / 48 h. after the biofilms were formed, they were exposed for 5 minutes to the solution ...201425000605
evaluation of the antimicrobial potency of silver nanoparticles biosynthesized by using an endophytic fungus, cryptosporiopsis ericae ps4.in the present study, silver nanoparticles (agnps) with an average particle size of 5.5 ± 3.1 nm were biosynthesized using an endophytic fungus cryptosporiopsis ericae ps4 isolated from the ethno-medicinal plant potentilla fulgens l. the nanoparticles were characterized using uv-visible spectrophotometer, transmission electron microscopy (tem), scanning electron microscopy (sem), selective area electron diffraction (saed), and energy dispersive x-ray (edx) spectroscopy analysis. antimicrobial ef ...201424994011
synergistic antimicrobial action of chlorhexidine and ozone in endodontic treatment.the aim of this study was to determine whether irrigation with sodium hypochlorite, chlorhexidine, and ozone gas, alone or in combination, were effective against enterococcus faecalis and candida albicans; these are microorganisms frequently isolated from teeth with periapical lesions resistant to endodontic treatment.201424982899
antimicrobial activity of cationic peptides in endodontic procedures.the present study aimed to investigate the antimicrobial and biofilm inhibition activity of synthetic antimicrobial peptides (amps) against microbes such as enterococcus faecalis, staphylococcus aureus, and candida albicans which are involved in endodontic infections.201424966779
endophytic fungi from myrcia guianensis at the brazilian amazon: distribution and bioactivity.beneficial interactions between plants and microorganisms have been investigated under different ecological, physiological, biochemical, and genetic aspects. however, the systematic exploration of biomolecules with potential for biotechnological products from this interaction still is relatively scarce. therefore, this study aimed the evaluation of the diversity and antimicrobial activity of the endophytic fungi obtained from roots, stems and leafs of myrcia guianensis (myrtaceae) from the brazi ...201424948926
effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine.the purpose of this in vitro study was to determine whether the addition of 2% chlorhexidine gluconate or 10% doxycycline would enhance the antimicrobial activity of biodentine against staphylococcus aureus (atcc-25923), enterococcus faecalis (atcc-29212), candida albicans (atcc-90028), and streptococcus mutans (mtcc-497).201424944453
microbial diversity in persistent root canal infections investigated by checkerboard dna-dna hybridization.the aim of the present study was to investigate the composition of the root canal microbiota in endodontic failures in order to identify and quantify these microorganisms.201424935532
antimicrobial and antifungal activity of pelargonium roseum essential oils.the antiseptic qualities of aromatic and medicinal plants and their extracts have been recognized since antiquity, while attempts to characterize these properties in the laboratory date back the beginning of the xx(th) century. in the current study essential oils obtained from pelargonium roseum (geraniacea) were analyzed for their antibacterial and antifungal activities.201425671182
'honey ointment': a natural remedy of skin wound infections.honey is a gift of nature, principally identified and valued to possess antimicrobial and anti-inflammatory activity and has been used as a natural remedy of wounds since ancient times. the objectives of this study were to evaluate the antimicrobial activity of honey against micro-organisms, to formulate a honey ointment and to evaluate the efficacy of such ointment by conducting clinical trials on skin wound infection.201424800336
essential oil composition and antimicrobial activity of angelica archangelica l. (apiaceae) roots.in this paper, the chemical composition and the antimicrobial activity of the essential oil of angelica archangelica l. (apiaceae) roots from central italy were analyzed. the major constituents of the oil were α-pinene (21.3%), δ-3-carene (16.5%), limonene (16.4%) and α-phellandrene (8.7%). the oil shows a good antimicrobial activity against clostridium difficile, clostridium perfringens, enterococcus faecalis, eubacterium limosum, peptostreptococcus anaerobius, and candida albicans with minimum ...201424788027
protection of mice from oral candidiasis by heat-killed enterococcus faecalis, possibly through its direct binding to candida albicans.to develop a new therapy against oral candidiasis, a commensal microorganism, enterococcus faecalis was tested for its ability to modulate candida growth in vitro and its therapeutic activities against a murine model in vivo. addition of heat-killed e. faecalis strain ef2001 (ef2001) isolated from healthy human feces to the culture of c. albicans strain timm1768 inhibited adherence of the latter to a microtiter plate in a dose dependent manner and candida cells surrounded by ef2001 were increase ...201424682096
impact of staphylococcus aureus on pathogenesis in polymicrobial infections.polymicrobial infections involving staphylococcus aureus exhibit enhanced disease severity and morbidity. we reviewed the nature of polymicrobial interactions between s. aureus and other bacterial, fungal, and viral cocolonizers. microbes that were frequently recovered from the infection site with s. aureus are haemophilus influenzae, enterococcus faecalis, pseudomonas aeruginosa, streptococcus pneumoniae, corynebacterium sp., lactobacillus sp., candida albicans, and influenza virus. detailed an ...201424643542
[development of polycomponent metabolite probiotic].construct composition of polycomponent probiotic based on lactobacilli cultural fluid (cf) supernatant.201424605659
novel benzoxazoles: synthesis and antibacterial, antifungal, and antitubercular activity against antibiotic-resistant and -sensitive microbes.a new series of 5-(p-substituted benzamido/phenylacetamido)-2-(p-tert-butylphenyl)benzoxazole derivatives were synthesized and evaluated for their antibacterial, antifungal, and antimycobacterial activities against antibiotic-resistant and -sensitive staphylococcus aureus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, and mycobacterium tuberculosis as well as against candida albicans and candida krusei. the compounds possessed broad-spectrum activity against all of the tested g ...201424601083
antimicrobial activity of the marine alkaloids, clathrodin and oroidin, and their synthetic analogues.marine organisms produce secondary metabolites that may be valuable for the development of novel drug leads as such and can also provide structural scaffolds for the design and synthesis of novel bioactive compounds. the marine alkaloids, clathrodin and oroidin, which were originally isolated from sponges of the genus, agelas, were prepared and evaluated for their antimicrobial activity against three bacterial strains (enterococcus faecalis, staphylococcus aureus and escherichia coli) and one fu ...201424534840
[investigation of antimicrobial and antibiofilm effects of some preservatives used in drugs, cosmetics and food products].preservatives are added to food, drugs and other pharmaceutical products to avoid microbial contamination. for antimicrobial activity testing and preservative efficacy testing, vegetative forms of the standard test organisms are used. however, microbial biofilm formation may occur on living tissues, medical implants, industrial or drinking water pipes, natural aquatic systems, glass and plastic surfaces. in our study, it was aimed to determine the antimicrobial and antibiofilm effects of some pr ...201424506719
evaluation of epidemiological cut-off values indicates that biocide resistant subpopulations are uncommon in natural isolates of clinically-relevant microorganisms.to date there are no clear criteria to determine whether a microbe is susceptible to biocides or not. as a starting point for distinguishing between wild-type and resistant organisms, we set out to determine the minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) distributions for four common biocides; triclosan, benzalkonium chloride, chlorhexidine and sodium hypochlorite for 3319 clinical isolates, with a particular focus on staphylococcus aureus (n = 1635) and ...201424466194
antimicrobial effects of calcium hydroxide, chlorhexidine, and propolis on enterococcus faecalis and candida albicans.to evaluate the efficacy of calcium hydroxide (ca[oh]2 ), 2% chlorhexidine (chx) gel, and propolis against both enterococcus faecalis (e. faecalis) and candida albicans (c. albicans) using infected dentine models at two different depths (100 and 200 μm) after 14 days of application.201423559565
effect of ottoman viper (montivipera xanthina (gray, 1849)) venom on various cancer cells and on microorganisms.cytotoxic and antimicrobial effects of montivipera xanthina venom against lncap, mcf-7, ht-29, saos-2, hep3b, vero cells and antimicrobial activity against selected bacterial and fungal species: staphylococcus aureus atcc 25923, escherichia coli atcc 25922, e. coli o157h7, enterococcus faecalis 29212, enterococcus faecium dsm 13590, staphylococcus epidermidis atcc 12228, s. typhimirium ccm 5445, proteus vulgaris atcc 6957 and candida albicans atcc 10239 were studied for evaluating the potential ...201423381026
the effect of temperature and contact time of sodium hypochlorite on human roots infected with enterococcus faecalis and candida albicans.the aim of this study was to evaluate the effectiveness of 2.5 % naocl at different temperature and time intervals on enterococcus faecalis and candida albicans-infected human roots. a total of 112 root cylinders prepared from extracted single-rooted humans were infected by e. faecalis (group a, n = 56) or c. albicans (group b, n = 56); 3 root cylinders served as negative controls. both groups were further divided into 6 subgroups according to three contact times (30 s, 1 min, 5 min) with naocl ...201423011474
diagnosis of pelvic inflammatory disease (pid): intra-operative findings and comparison of vaginal and intra-abdominal cultures.pelvic inflammatory disease (pid) is frequent in adolescents and younger women. diagnosis is usually based on the clinical findings, and the threshold for empiric antibiotic therapy should be low. however, at least in cases of resistance toward therapy or deterioration of symptoms, laparoscopic evaluation can be helpful.201424474636
inter- and intraspecies transfer of a clostridium difficile conjugative transposon conferring resistance to mlsb.resistance to the macrolide-lincosamide-streptogramin b group of antibiotics in clostridium difficile is generally due to erm(b) genes. tn6194, a conjugative transposon initially detected in pcr-ribotype 027 isolates, is an erm(b)-containing element also detected in other relevant c. difficile pcr-ribotypes. in this study, the genome of a c. difficile pcr-ribotype 001 strain was sequenced, and an element with two nucleotidic changes compared to tn6194 was detected. this element was transferred b ...201425055190
surotomycin demonstrates low in vitro frequency of resistance and rapid bactericidal activity in clostridium difficile, enterococcus faecalis, and enterococcus faecium.surotomycin (cb-183,315) is an orally administered, minimally absorbed, selective bactericidal cyclic lipopeptide in phase 3 development for the treatment of clostridium difficile-associated diarrhea. the aim of this study was to evaluate the emergence of resistance in c. difficile (atcc 700057 and three recent clinical isolates from the restriction endonuclease analysis groups bi, bk, and k), vancomycin-susceptible (vs) enterococcus faecalis (atcc 49452), vancomycin-resistant (vr) e. faecalis ( ...201424798273
screening of bacteriocin-producing enterococcus faecalis strains for antagonistic activities against clostridium perfringens.this study was conducted to isolate and characterize bacteriocin-producing bacteria against clostridium perfringens (c. perfringens) from domestic animals to determine their usefulness as probiotics. bacteriocin-producing bacteria were isolated from pig feces by the spot-on-lawn method. a total of 1,370 bacterial stains were isolated, and six were tentatively selected after identifying the inhibitory activity against the pathogenic indicator c. perfringens kctc 3269 and kctc 5100. the selected s ...201426761495
technological properties of bacteriocin-producing lactic acid bacteria isolated from pico cheese an artisanal cow's milk cheese.evaluate technologically relevant properties from bacteriocin-producing strains to use as starter/adjunct cultures in cheese making.201424206097
chronic botulism in a saxony dairy farm: sources, predisposing factors, development of the disease and treatment possibilities.the aim of this study is to investigate clostridium botulinum at a saxony dairy farm with 159 cows and 18 heifers. the animals exhibited clinical symptoms of chronic botulism. to determine the source of the infection, feces, blood, organs, and gastrointestinal fluids of dead or euthanized cows; as well as soil, water, silage and manure were tested for c. botulinum spores and bonts using elisa. bont/c and c. botulinum type c were detected in 53% and 3% of tested animals, respectively, while bont/ ...201424997242
clinical epidemiological study on the incidence of escherichia coli infections in the cancer patients admitted to surgery department ii of the iasi regional oncology institute in 2013.the aim of study was to identify pathogens involved in hospital-acquired or community-acquired infections in patients suffering from various types of cancers.201425341303
prevalence of antibacterial resistant bacterial contaminants from mobile phones of hospital inpatients.mobile phones contaminated with bacteria may act as fomites. antibiotic resistant bacterial contamination of mobile phones of inpatients was studied. one hundred and six samples were collected from mobile phones of patients admitted in various hospitals in jazan province of saudi arabia. eighty-nine (83.9%) out of 106 mobile phones were found to be contaminated with bacteria. fifty-two (49.0%) coagulase-negative staphylococcus, 12 (11.3%) staphylococcus aureus, 7 (6.6%) enterobacter cloacae, 3 ( ...201428156258
safety of topical vancomycin for pediatric spinal deformity: nontoxic serum levels with supratherapeutic drain levels.retrospective cohort analysis.201424921849
postoperative spine and veptr infections in children: a case-control study.surgical site infection (ssi) after pediatric scoliosis surgery is a major cause of morbidity. we compared the odds ratios of various potential risk factors for infection among patients who developed a deep ssi following spinal deformity surgery and those who remained infection free.201424327165
the effectiveness of enzymic irrigation in removing a nutrient-stressed endodontic multispecies biofilm.to establish a nutrient-stressed multispecies model biofilm and investigate the dynamics of biofilm killing and disruption by 1% trypsin and 1% proteinase k with or without ultrasonic activation.201424246147
new antimicrobial pregnane glycosides from the stem of ecdysanthera rosea.phytochemical investigation on the stem of ecdysanthera rosea led to the isolation of eight new c-21 pregnane glycoside ecdysosides a-h (1-8), together with one known pregnane glycoside ecdysantheroside a (9). their structures were elucidated based on extensive spectroscopic data (ms, ir, 1d and 2d nmr) analysis, as well as comparison with the reported literature data. antimicrobial activities of all the compounds were evaluated against bacteria and yeasts. compounds 1, 9, 3 and 5 exhibited mode ...201425454459
methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococcus faecalis and enterococcus faecium active dimeric isobutyrylphloroglucinol from ivesia gordonii.bioassay-guided fractionation of the chloroform soluble fraction of stem, leaf, and flower extracts of the american plant ivesia gordonii led to the isolation of a new dimeric acylphloroglucinol, 3,3'-diisobutyryl-2,6'-dimethoxy-4,6,2',4'-tetrahydroxy-5,5'dimethyldiphenyl methane (1), to which we have assigned the trivial name ofivesinol (1), together with a known monomeric acylphloroglucinol, 1,5-dihydroxy-2-(2'-methylpropionyl)-3-methoxy-6-methylbenzene (2). the structures of the isolated comp ...201424689296
enterococcus--virulence and susceptibility to photodynamic therapy of clinical isolates from lower silesia, poland.a collection of 400 enterococcal isolates from clinical samples of hospitalized patients were studied for their virulence traits according to the isolation site. formation of biofilm and production of dnase, hemolysin, lipase, and gelatinase were characterized. biofilms of selected strains were examined for their susceptibility to antimicrobial photodynamic therapy (apdt).201425290581
antimicrobial susceptibility of enterococcal species isolated from antibiotic-treated dogs and cats.in this study, we examined the antimicrobial susceptibility of the enterococci isolated from dogs and cats in japan during 2011-2012. fecal samples were collected from 84 dogs and 16 cats that underwent antibiotic treatment. enterococci were detected in 70 of 84 dogs (83.3%) and 7 of 16 cats (43.8%). the most prevalent enterococcus species was enterococcus faecalis (64.9%); enterococcus faecium and enterococcus durans were also isolated from 14 of 77 (18.2%) and 5 of 77 (6.5%) of these animals, ...201424976587
prevalence of enterococcus species and their virulence genes in fresh water prior to and after storm events.enterococcus spp. isolates (n = 286) collected from six surface water bodies in subtropical brisbane, australia, prior to and after storm events, were identified to species level and tested for the presence of seven clinically important virulence genes (vgs). enterococcus faecalis (48%), enterococcus faecium (14%), enterococcus mundtii (13%), and enterococcus casseliflavus (13%) were frequently detected at all sites. the frequency of e. faecium occurrence increased from 6% in the dry period to 1 ...201424494806
animal rennets as sources of dairy lactic acid bacteria.the microbial composition of artisan and industrial animal rennet pastes was studied by using both culture-dependent and -independent approaches. pyrosequencing targeting the 16s rrna gene allowed to identify 361 operational taxonomic units (otus) to the genus/species level. among lactic acid bacteria (lab), streptococcus thermophilus and some lactobacilli, mainly lactobacillus crispatus and lactobacillus reuteri, were the most abundant species, with differences among the samples. twelve groups ...201424441167
significance and survival of enterococci during the house fly development.house flies are among the most important nonbiting insect pests of medical and veterinary importance. larvae develop in decaying organic substrates and their survival strictly depends on an active microbial community. house flies have been implicated in the ecology and transmission of enterococci, including multi-antibiotic-resistant and virulent strains of enterococcus faecalis. in this study, eight american type culture collection type strains of enterococci including enterococcus avium, enter ...201424605454
high inhibition of paenibacillus larvae and listeria monocytogenes by enterococcus isolated from different sources in tunisia and identification of their bacteriocin genes.a total of 300 isolates of enterococcus, from different sources including faeces of poultry, cow and sheep, raw milk, ricotta cheese and water, in tunisia, were screened for their antibacterial activity. amongst them, 59 bacteriocin-producing strains were detected and identified by molecular methods. genes encoding for enta, entp, entb, entl50a/b, as-48 and bac31 bacteriocins were targeted by pcr. the bacteriocin-producing strains were assigned to the species enterococcus faecium, enterococcus f ...201424698417
bacteriocinogenic properties and in vitro probiotic potential of enterococci from tunisian dairy products.the aim of this study was to isolate new bacteriocinogenic strains with putative probiotic potential from various tunisian fermented milks. a total of 44 gram-positive catalase-negative isolates were colony-purified and screened for antimicrobial activity. of inhibitory isolates, four were identified as enterococcus durans and one as enterococcus faecalis using 16s rrna gene sequence. the five strains were sensitive to penicillin g, all aminoglycosides tested, to the vancomycin, tetracycline, an ...201424627155
is there a need for bacterial endocarditis prophylaxis in patients undergoing gastrointestinal endoscopy?heart valve repair or replacement is a serious problem. patients can benefit from an open dialogue between both cardiologists and gastroenterologists for the optimal effective patients care. the focused update on infective endocarditis of the american college of cardiology/american heart association 2008 (acc/aha guidelines) and guidelines on the prevention, diagnosis, and treatment of infective endocarditis (new version 2009) of the european society of cardiology (esc guidelines) describe proph ...201424566725
characterization of lactic acid bacteria isolated from artisanal travnik young cheeses, sweet creams and sweet kajmaks over four seasons.the aim of this study was to investigate the composition of lactic acid bacteria (lab) in autochthonous young cheeses, sweet creams and sweet kajmaks produced in the vlašić mountain region of central bosnia and herzegovina near the town of travnik over a four season period. these three products were made from cow's milk by a traditional method without the addition of a starter culture. preliminary characterization with phenotype-based assays and identification using rep-pcr with a (gtg)5 primer ...201424387849
phenotypic expression, molecular characterization and transferability of erythromycin resistance genes in enterococcus spp. isolated from naturally fermented food.to evaluate phenotypic resistance to macrolides-lincosamides and streptogramin b (mlsb ) antibiotics and to determine their localization as well as transferability of erythromycin resistance genes in enterococcal isolates of naturally fermented food-idli batter.201424206069
clinical evaluation of sustained-release metronidazole gel versus metronidazole solution as an intracanal medicament in abscessed primary molars.to evaluate the efficacy of metronidazole gel versus metronidazole solution against enterococcus faecalis in abscessed primary molars.201423754205
effects of enterococcus faecalis lipoteichoic acid on receptor activator of nuclear factor-κb ligand and osteoprotegerin expression in periodontal ligament fibroblasts.to investigate the influence of enterococcus faecalis lipoteichoic acid (lta) on the key bone resorption-regulating proteins, receptor activator of nuclear factor-κb ligand (rankl) and osteoprotegerin (opg) in human periodontal ligament fibroblasts (pdl cells).201423710898
the cell wall-targeting antibiotic stimulon of enterococcus faecalis.enterococcus faecalis is an opportunistic nosocomial pathogen that is highly resistant to a variety of environmental insults, including an intrinsic tolerance to antimicrobials that target the cell wall (cw). with the goal of determining the cw-stress stimulon of e. faecalis, the global transcriptional profile of e. faecalis og1rf exposed to ampicillin, bacitracin, cephalotin or vancomycin was obtained via microarrays. exposure to the β-lactams ampicillin and cephalotin resulted in the fewest tr ...201423755154
bacterial colonization of microbial biofilms in oral squamous cell carcinoma.the purpose of this prospective clinical study was to identify the bacterial spectra on the surface of oral squamous cell carcinomas (oscc) in comparison to oral mucosa of patients with a higher risk to emerge an oscc and a control group to determine their susceptibility to various common antibiotics.201423793404
differential efficacy of endodontic obturation procedures: an ex vivo study.by means of a double-chamber model, different root canal filling materials and procedures were compared. briefly, the root canals of single-rooted human teeth, extracted for periodontal reasons, were instrumented and obturated by gutta-percha/pulp canal sealer ewt (pcs) or by resilon, in association with different sealers (real seal, relyx unicem or meta). obturation was achieved by traditional continuous wave of condensation technique (tcwct), a modified version of it (mcwct), or single cone te ...201423836051
oral application of bacterial lysate in infancy diminishes the prevalence of atopic dermatitis in children at risk for atopy.numerous interventions such as avoidance of food allergens, prolonged breast feeding and supplementation of pro-and/or prebiotics have been tried as primary prevention of atopic dermatitis. recent data suggest that prevention of infantile eczema is possible in a subgroup of children by feeding bacterial lysates early in life. bacterial lysates of escherichia coli and enterococcus faecalis were found to impair allergic immune responses in rats. an interventional trial in 606 infants at risk for a ...201423886978
effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen.to study how products released from different bacteria in a deep carious lesion affect the metabolic activity of odontoblast-like cells and their ability to produce the major organic component of dentine, collagen 1.201423902462
antimicrobial activity of a uv-stable bacteriocin-like inhibitory substance (blis) produced by enterococcus faecium strain dsh20 against vancomycin-resistant enterococcus (vre) strains.the narrow spectrum of action of most bacteriocins is an important limitation for their application as antimicrobial agents. the current study describes a novel bacteriocin-like inhibitory substance (blis) that display extended spectrum antimicrobial activity against vancomycin-resistant enterococcus (vre) strains.201423838264
american crows as carriers of vancomycin-resistant enterococci with vana gene.we studied the vana-carrying vancomycin-resistant enterococci (vre) isolated from american crows in the united states during the winter 2011/2012. faecal samples from crows were cultured selectively for vre and characterized. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) were used to examine epidemiological relationships of vana-containing vre. isolates were tested in vitro for their ability to horizontally transfer the vancomycin resistance trait. vre with the va ...201423919480
interdigitated silver-polymer-based antibacterial surface system activated by oligodynamic iontophoresis - an empirical characterization study.there is a pressing need to control the occurrences of nosocomial infections due to their detrimental effects on patient well-being and the rising treatment costs. to prevent the contact transmission of such infections via health-critical surfaces, a prophylactic surface system that consists of an interdigitated array of oppositely charged silver electrodes with polymer separations and utilizes oligodynamic iontophoresis has been recently developed. this paper presents a systematic study that em ...201423917746
bimix antimicrobial scaffolds for regenerative endodontics.eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. the aim of this study was to synthesize and determine in vitro both the antimicrobial effectiveness and cytocompatibility of bimix antibiotic-containing polydioxanone-based polymer scaffolds.201425201643
structure of the double-stranded dna-binding type iv secretion protein tran from enterococcus.conjugative transfer through type iv secretion multiprotein complexes is the most important means of spreading antimicrobial resistance. plasmid pip501, frequently found in clinical enterococcus faecalis and enterococcus faecium isolates, is the first gram-positive (g+) conjugative plasmid for which self-transfer to gram-negative (g-) bacteria has been demonstrated. the pip501-encoded type iv secretion system (t4ss) protein tran localizes to the cytoplasm and shows specific dna binding. the spec ...201425195751
the effect of sodium hypochlorite on enterococcus faecalis when grown on dentine as a single- and multi-species biofilm.enterococcus faecalis is often involved in the aetiology of apical periodontitis after endodontic treatment. this project aimed to establish, on dentine in vitro, a multi-species biofilm containing e. faecalis, and to determine if the organism had an increased resistance to sodium hypochlorite compared with an axenic biofilm. biofilms were established on dentine discs in flow cells with either e. faecalis alone (axenic) or together with fusobacterium nucleatum and streptococcus sanguinis. follow ...201425195495
daptomycin in the treatment of prosthetic joint infection by enterococcus faecalis: safety and efficacy of high-dose and prolonged therapy.enterococci are implicated in less than 2.3% of prosthetic joint infections. these infections can be difficult to treat and therapeutic failures are not uncommon. in these situations, daptomycin is a safe and effective alternative. we present a clinical case with a successful response to the prolonged use of high-dose daptomycin.201425192907
development of an antibacterial root canal filling system containing mdpb.an antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (mdpb)-containing experimental, chemically cured primer was prepared to develop a new resin-based root canal filling system. this study investigated the antibacterial effects of the mdpb-containing primer (experimental primer [ep]) against enterococcus faecalis and assessed the in vitro bonding and sealing abilities of the filling system, consisting of ep and a bis-gma-based sealer resin. antibacterial effects of ep were evaluat ...201425192898
the cyclic dinucleotide c-di-amp is an allosteric regulator of metabolic enzyme function.cyclic di-adenosine monophosphate (c-di-amp) is a broadly conserved second messenger required for bacterial growth and infection. however, the molecular mechanisms of c-di-amp signaling are still poorly understood. using a chemical proteomics screen for c-di-amp-interacting proteins in the pathogen listeria monocytogenes, we identified several broadly conserved protein receptors, including the central metabolic enzyme pyruvate carboxylase (lmpc). biochemical and crystallographic studies of the l ...201425215494
draft genome sequence of an enterococcus faecalis strain isolated from a neonatal blood sepsis patient.herein, we report the draft genome sequence of enterococcus faecalis ed-ngs-1009, cultivated from a blood sample taken from a neonatal sepsis patient at the royal infirmary in edinburgh, scotland, united kingdom.201425212626
healthcare-associated infective endocarditis: a case series in a referral hospital from 2006 to 2011.background: healthcare-associated infective endocarditis (hca-ie), a severe complication of medical care, shows a growing incidence in literature. objective: to evaluate epidemiology, etiology, risk factors for acquisition, complications, surgical treatment, and outcome of hca-ie. methods: observational prospective case series study (2006-2011) in a public hospital in rio de janeiro. results: fifty-three patients with hca-ie from a total of 151 cases of infective endocarditis (ie) were included. ...201425211203
driving forces of vancomycin-resistant e. faecium and e. faecalis blood-stream infections in children.rates of invasive vancomycin-resistant enterococcus (vre) in the usa remains on the rise. efforts to control vancomycin use and nosocomial transmission have had limited success in halting the spread of this pathogen. the role of antibiotic exposure remains a topic of controversy. we evaluated the association between emergence of vre-blood-stream infections (bsi), aggregate and individual-patient vancomycin- exposure, and clonal transmission of vre at an academic pediatric tertiary care hospital.201425206975
using mineral trioxide aggregate powder as an apical barrier: a bacterial leakage study.mineral trioxide aggregate (mta) has been used successfully for the treatment of necrotic immature teeth as part of a 2-step apical barrier. recently, some researchers have used mta powder instead of wet-mix mta. it has also been used in a single-step approach in order to decrease the number of patient appointments. the aim of this study was to evaluate the bacterial microleakage of mta powder in comparison with wet-mix mta, in single or 2-step barrier approaches. this ex vivo study was carried ...201425184718
antimicrobial activity of some essential oils against oral multidrug-resistant enterococcus faecalis in both planktonic and biofilm state.to evaluate some essential oils in treatment of intractable oral infections, principally caused by biofilm of multidrug-resistant enterococcus faecalis (e. faecalis), such as persistent endodontic infections in which their treatment exhibits a real challenge for dentists.201425182948
antibacterial validation of electrogenerated hypochlorite using carbon-based electrodes.this proof-of-concept study explores the novel use of carbon-based electrodes for the electrochemical generation of hypochlorite and compares the antimicrobial efficacy against commercial hypochlorite solution. antimicrobial concentrations of hypochlorite were generated using pad-printed carbon and carbon fibre electrodes, yielding up to 0·027% hypochlorite in 60 min and 0·1% hypochlorite in 15 min, respectively, in a nondivided assembly. the minimum inhibitory concentration (mic) of the electro ...201425179583
control of tyramine and histamine accumulation by lactic acid bacteria using bacteriocin forming lactococci.the aim of this study was to evaluate the competitive effects of three bacteriocin producing strains of lactococcus lactis subsp. lactis against two aminobiogenic lactic acid bacteria, i.e. the tyramine producing strain enterococcus faecalis ef37 and the histamine producing strain streptococcus thermophilus pri60, inoculated at different initial concentrations (from 2 to 6 log cfu/ml). the results showed that the three l. lactis subsp. lactis strains were able to produce bacteriocins: in particu ...201425173915
enterococcus faecalis infection and reactive oxygen species down-regulates the mir-17-92 cluster in gastric adenocarcinoma cell culture.chronic inflammation due to bacterial overgrowth of the stomach predisposes to the development of gastric cancer and is also associated with high levels of reactive oxygen species (ros). in recent years increasing attention has been drawn to micrornas (mirnas) due to their role in the pathogenesis of many human diseases including gastric cancer. here we studied the impact of infection by the gram-positive bacteria enterococcus faecalis (e. faecalis) on global mirna expression as well as the effe ...201425170597
vapor-induced transfer of bacteria in the absence of mechanical disturbances.transfer of bacteria through water vapor generated at moderate temperatures (30-50°c) in passive solar stills, has scarcely been reported. the objective of this research was to investigate whether bacteria in highly humid atmospheres can get transferred through water vapor in the absence of other transfer media to find their way to the distillate. to achieve this objective, passive solar reactors were chosen as the medium for experimentation, and distillation experiments were conducted by spikin ...201425169809
the influence of urinary ph on antibiotic efficacy against bacterial uropathogens.to determine the effects of ph on the activity of clinically relevant antibiotics against bacterial uropathogens. numerous factors affect antibiotic efficacy within the urinary tract including ph. because human urine can substantially vary from acidic (ph 4.5) to alkaline (ph 8) conditions and can be easily clinically manipulated, it would be a great advantage to better understand the role of ph in antibiotic treatment of urinary tract infection.201425168568
what's new in the treatment of enterococcal endocarditis?enterococcus spp. are among the common pathogens causing infective endocarditis (ie). despite major medical advances and new potent antimicrobial agents, the mortality has not significantly improved for several decades. the usual lack of bactericidal activity of penicillin or ampicillin, the toxicity from the combination of penicillin plus aminoglycosides, and the increased reports of high-level resistance to aminoglycosides have led to the exploration of other regimens for treatment of enteroco ...201425165018
evaluation of enterococcus faecalis clinical isolates with 'penicillin-resistant, ampicillin-susceptible' phenotype as reported by vitek-2 compact system.it has been recently reported that ampicillin susceptibility cannot accurately predict piperacillin and imipenem susceptibilities in penicillin-resistant, ampicillin-susceptible (pen-r, amp-s) enterococcus faecalis isolates, contrary to the current clinical and laboratory standards institute (clsi) recommendations. this has important therapeutic implications. such isolates were noted after the use of vitek-2 compact system ast-gp67 susceptibility cards in a singapore general hospital and they we ...201425158809
structures of new phenolics isolated from licorice, and the effectiveness of licorice phenolics on vancomycin-resistant enterococci.licorice, which is the underground part of glycyrrhiza species, has been used widely in asian and western countries as a traditional medicine and as a food additive. our continuous investigation on the constituents of roots and stolons of glycyrrhiza uralensis led to the isolation of two new phenolics, in addition to 14 known compounds. structural studies including spectroscopic and simple chemical derivatizations revealed that both of the new compounds had 2-aryl-3-methylbenzofuran structures. ...201425157467
a propidium monoazide-quantitative pcr method for the detection and quantification of viable enterococcus faecalis in large-volume samples of marine waters.the development of rapid detection assays of cell viability is essential for monitoring the microbiological quality of water systems. coupling propidium monoazide with quantitative pcr (pma-qpcr) has been successfully applied in different studies for the detection and quantification of viable cells in small-volume samples (0.25-1.00 ml), but it has not been evaluated sufficiently in marine environments or in large-volume samples. in this study, we successfully integrated blue light-emitting diod ...201425149448
bacterial removal promoted by 2 single-file systems: wave one and one shape.different single-file systems are available for endodontic treatment; however, comparative studies are scarce. thus, the present study evaluated bacterial reduction promoted by 2 single-file systems: wave one (dentsply maillefer, ballaigues, switzerland) and one shape (micromega, besancon, france).201425149120
in vitro assembly of catalase.most aerobic organisms contain catalase, which functions to decompose hydrogen peroxide. typical catalases are structurally complex homo-tetrameric enzymes with one heme prosthetic group buried in each subunit. it is not known how catalase in the cell is assembled from its constituents. the bacterium enterococcus faecalis cannot synthesize heme but can acquire it from the environment to form a cytoplasmic catalase. we have in e. faecalis monitored production of the enzyme polypeptide (kata) depe ...201425148685
wide distribution of virulence genes among enterococcus faecium and enterococcus faecalis clinical isolates.enterococcus, a gram-positive facultative anaerobic cocci belonging to the lactic acid bacteria of the phylum firmicutes, is known to be able to resist a wide range of hostile conditions such as different ph levels, high concentration of nacl (6.5%), and the extended temperatures between 5(°)c and 65(°)c. despite being the third most common nosocomial pathogen, our understanding on its virulence factors is still poorly understood. the current study was aimed to determine the prevalence of differ ...201425147855
riboswitches. a riboswitch-containing srna controls gene expression by sequestration of a response regulator.the ethanolamine utilization (eut) locus of enterococcus faecalis, containing at least 19 genes distributed over four polycistronic messenger rnas, appears to be regulated by a single adenosyl cobalamine (adocbl)-responsive riboswitch. we report that the adocbl-binding riboswitch is part of a small, trans-acting rna, eutx, which additionally contains a dual-hairpin substrate for the rna binding-response regulator, eutv. in the absence of adocbl, eutx uses this structure to sequester eutv. eutv i ...201425146291
nosocomial infection caused by vancomycin-susceptible multidrug-resistant enterococcus faecalis over a long period in a university hospital in japan.compared with other developed countries, vancomycin-resistant enterococci (vre) are not widespread in clinical environments in japan. there have been no vre outbreaks and only a few vre strains have sporadically been isolated in our university hospital in gunma, japan. to examine the drug susceptibility of enterococcus faecalis and nosocomial infection caused by non-vre strains, a retrospective surveillance was conducted in our university hospital. molecular epidemiological analyses were perform ...201425145983
a comparison of conventional methods for the quantification of bacterial cells after exposure to metal oxide nanoparticles.due to potential interference of nanoparticles on bacterial quantification, there is a challenge to develop a fast, accurate and reproducible method for bacterial quantification. currently various bacterial quantification methods are used by researchers performing nanoparticles study, but there has been no efficacy evaluation of these methods. here we study interference of nanoparticles on three most commonly used conventional bacterial quantification methods, including colony counting to determ ...201425138641
molecular characterization of streptococcus agalactiae isolates harboring small erm(t)-carrying plasmids.among 1,827 group b streptococcus (gbs) strains collected between 2006 and 2013 by the french national reference center for streptococci, 490 (26.8%) strains were erythromycin resistant. the erm(t) resistance gene was found in six strains belonging to capsular polysaccharides ia, iii, and v and was carried by the same mobilizable plasmid, which could be efficiently transferred by mobilization to gbs and enterococcus faecalis recipients, thus promoting a broad dissemination of erm(t).201425136004
a simple and effective method to synthesize fluorescent nanoparticles using tryptophan and light and their lethal effect against bacteria.a simple, environmentally friendly and cost-effective method was used to synthesize silver nanoparticles using tryptophan and light. to prepare the nanoparticles, the following components were used: deionized water, silver nitrate, light and tryptophan. the effects of the tryptophan concentration and light exposure time on the formation of tryptophan silver nanoparticles (tnnps) were studied. the synthesized tnnps were characterized using transmission electron microscopy (tem), absorption and fl ...201425129701
antibacterial phototoxic effects of synthetic asymmetric and glycosylated curcuminoids in aqueous formulations: studies on curcumin and curcuminoids. liv.the aim of this study was to evaluate the in vitro phototoxic potential of synthetic asymmetric and glycosylated curcuminoids on planktonic model bacteria by counting the colony forming units. the gram-positive enterococcus faecalis and the gram-negative escherichia coli were exposed to aqueous solutions of the curcuminoids (⩽2.5 μm) in the presence or absence of selected pharmaceutical excipients (pluronic f127, peg 400 and hpγcd) in combination with a low irradiation dose (5 j/cm(2); λmax: 450 ...201425129700
incorporation of exogenous fatty acids protects enterococcus faecalis from membrane-damaging agents.enterococcus faecalis is a commensal bacterium of the mammalian intestine that can persist in soil and aquatic systems and can be a nosocomial pathogen to humans. it employs multiple stress adaptation strategies in order to survive such a wide range of environments. within this study, we sought to elucidate whether membrane fatty acid composition changes are an important component for stress adaptation. we noted that e. faecalis og1rf was capable of changing its membrane composition depending up ...201425128342
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