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genetic environments of the transferable plasmid-mediated blactx-m-3 gene in serratia marcescens isolates.in this study, genetic environments of the transferable plasmid-mediated blactx-m-3 gene were characterized among 14 isolates of cefotaxime-resistant serratia marcescens using pcr and blast dna sequence analysis. a total of 3 types of genetic architectures in the regions surrounding this blactx-m-3 gene were identified. type i architecture was characterized by the presence of a complete insertion sequence of tnpa-isecp1, identified as interrupting a reverse is26 sequence in the upstream region o ...201424451105
the genome and proteome of serratia bacteriophage η which forms unstable lysogens.serratia marcescens phage η is a temperate unclassified member of the siphoviridae which had been reported as containing hypermodified guanine residues.201424433577
serratia marcescens suppresses host cellular immunity via the production of an adhesion-inhibitory factor against immunosurveillance cells.injection of a culture supernatant of serratia marcescens into the bloodstream of the silkworm bombyx mori increased the number of freely circulating immunosurveillance cells (hemocytes). using a bioassay with live silkworms, serralysin metalloprotease was purified from the culture supernatant and identified as the factor responsible for this activity. serralysin inhibited the in vitro attachment of both silkworm hemocytes and murine peritoneal macrophages. incubation of silkworm hemocytes or mu ...201424398686
microbial production of 2,3-butanediol by a newly-isolated strain of serratia marcescens.a newly-isolated strain of serratia marcescens, g12, was characterized for 2,3-butanediol (2,3-bd) production. in shake-flask and batch fermentations, 2,3-bd reached 48.5 and 51 g l(-1), respectively. low amounts of (~8 g l(-1)) of acetoin were also formed. in fed-batch fermentations, strain g12 produced 72.8 g 2,3-bd l(-1) with glucose initially at 130 g l(-1). when aeration rate was increased to 2.5 vvm for the fermentation process, 2,3-bd reached 87.8 g l(-1) and the highest productivity was ...201424375234
use of quantitative real-time pcr for direct detection of serratia marcescens in marine and other aquatic environments.serratia marcescens is the etiological agent of acroporid serratiosis, a distinct form of white pox disease in the threatened coral acropora palmata. the pathogen is commonly found in untreated human waste in the florida keys, which may contaminate both nearshore and offshore waters. currently there is no direct method for detection of this bacterium in the aquatic or reef environment, and culture-based techniques may underestimate its abundance in marine waters. a quantitative real-time pcr ass ...201424375136
rapid evolutionary adaptation to elevated salt concentrations in pathogenic freshwater bacteria serratia marcescens.rapid evolutionary adaptions to new and previously detrimental environmental conditions can increase the risk of invasion by novel pathogens. we tested this hypothesis with a 133-day-long evolutionary experiment studying the evolution of the pathogenic serratia marcescens bacterium at salinity niche boundary and in fluctuating conditions. we found that s. marcescens evolved at harsh (80 g/l) and extreme (100 g/l) salt conditions had clearly improved salt tolerance than those evolved in the other ...201425505519
[bacteremia by a serratia rubidaea with an atypical quinolones resistance phenotype].serratia spp. causes the 2% of nosocomial infections. serratia marcescens is the pathogenic species for excellence as it causes more than 90% of cases, however, there are very few reported cases of clinical infection caused by other minority species, among which is s. rubidaea. we present a case of nosocomial bacteremia by s. rubidaea, probably related to a peripheral catheter, in a healthy teenager who consulted to the emergency room after suffering a fit while practicing strenuous sport.201425146213
non-pigmented strain of serratia marcescens: an unusual pathogen causing pulmonary infection in a patient with malignancy.serratia marcescens is a member of the family enterobacteriaceae. it has emerged in recent years as an opportunistic pathogen of nosocomial infections. some biotypes of serratia marcescens produce the non-diffusible red pigment prodigiosin. though both pigmented and non-pigmented biotypes may be pathogenic for humans, the non-pigmented biotypes are more virulent due to cytotoxin production and presence of plasmids mediating antibiotic resistance. however in india only one study done 31 years bac ...201425120985
magnetic steering control of multi-cellular bio-hybrid microswimmers.bio-hybrid devices, which integrate biological cells with synthetic components, have opened a new path in miniaturized systems with the potential to provide actuation and control for systems down to a few microns in size. here, we address the challenge of remotely controlling bio-hybrid microswimmers propelled by multiple bacterial cells. these devices have been proposed as a viable method for targeted drug delivery but have also been shown to exhibit stochastic motion. we demonstrate a method o ...201425120224
serratia marcescens spinal epidural abscess formation following acupuncture.the formation of spinal epidural abscess following acupuncture is very rare. we herein report the case of a 54-year-old woman who presented with progressive low back pain and fever with a root sign. she underwent surgical decompression, with an immediate improvement of the low back pain. a culture of the epidural abscess grew serratia marcescens. one year postoperatively, magnetic resonance imaging revealed the almost complete eradication of the abscess. this case is the first case of serratia m ...201425088883
the story of serratia marcescens: pathologic risk factors in breast implant surgery.serratia marcescens (s. marcescens) emerged as an opportunist in the setting of immunodeficiency in the 1970s, when serious infections occurred in san francisco hospitals after usa. navy experiments had aerosolized the bacteria to study biologic warfare. we investigate the risks of s. marcescens in san franciscans who undergo mastectomy with implant reconstruction. from 2007 to 2011, the senior author took breast capsule cultures for all patients at the time of tissue expander exchange/explant. ...201425075367
genome evolution and plasticity of serratia marcescens, an important multidrug-resistant nosocomial pathogen.serratia marcescens is an important nosocomial pathogen that can cause an array of infections, most notably of the urinary tract and bloodstream. naturally, it is found in many environmental niches, and is capable of infecting plants and animals. the emergence and spread of multidrug-resistant strains producing extended-spectrum or metallo beta-lactamases now pose a threat to public health worldwide. here we report the complete genome sequences of two carefully selected s. marcescens strains, a ...201425070509
experimental coevolution: rapid local adaptation by parasites depends on host mating system.host-parasite interactions can drive rapid, reciprocal genetic changes (coevolution), provided both hosts and parasites have high heritabilities for resistance/infectivity. similarly, the host's mating system should also affect the rate of coevolutionary change in host-parasite interactions. using experimental coevolution, we determined the effect of obligate outcrossing verses partial self-fertilization (mixed mating) on the rate of evolutionary change in a nematode host (caenorhabditis elegans ...201425061681
studies on growth kinetics of serratia marcescens vitsd2 and optimization of fermentation conditions for serratiopeptidase production.serratia is one of the most important groups of bacteria which produces proteolytic enzymes effectively and known to possess anti- inflammatory properties. the main focus of the current study was to optimize the culture conditions of serratia marcescens vitsd2 for the mass production of serratiopeptidase. effect of various nutritional and environmental factors were analysed and optimized. among the different carbon and nitrogen sources tested, mannose and soya bean meal was found to be the best ...201425056366
construction and basic characterization of deletion mutants of the genes involved in chitin utilization by serratia marcescens 2170.in order to elucidate the roles of chip, chiq, and chix in chitin utilization by serratia marcescens 2170, the construction of single-gene deletion mutants of the chip, chiq, and chix genes was attempted by allelic exchange mutagenesis. δchip formed smaller clearing zones and δchix formed larger ones than wild-type 2170 on an agar plate containing colloidal chitin. δchip grew slowly on the lower concentration of (glcnac)2, and there was essentially no growth on chitin oligosaccharides larger tha ...201425036845
identification and crude protein extract of endophytic bacteria strain klxd06 antagonistic against mrsa isolated from hemsleya sinesis.hemsleya sinesis cogn has a variety of medicinal value. strain klxd06 is an endophytic bacteria isolated from h. sinesis exhibited significant inhibitory activity against methicillin-resistant staphylococcus aureus (mrsa). this strain was identified by methods including 16s rdna sequence homology and phylogenetic analysis morphological, biochemical and physiological characteristics analysis. ccrude protein from klxd06 was extracted by ammon ium sulfate salting-out. the results showed that strain ...201425016271
recurrent outbreaks of serratia marcescens among neonates and infants at a pediatric department: an outbreak analysis.serratia marcescens is a known cause of bloodstream infections (bsis) and outbreaks in neonates receiving intensive care. our aim was to analyze clinical and epidemiological characteristics of two outbreaks detected in our unit to prevent and control further epidemic infections.201425015432
the lack of ompf, but not ompc, contributes to increased antibiotic resistance in serratia marcescens.the environmental organism serratia marcescens is one of the primary causes of numerous nosocomial outbreaks and opportunistic infections. multi-drug resistance is now a common feature among s. marcescens clinical isolates, complicating the efficacy of treatment. recent reports have attributed antibiotic resistance to altered porin expression as well as perturbation of the intrinsic ampc beta-lactamase production pathway. in this study, we aimed to genetically correlate the absence of ompf and o ...201425015362
first detection of a metallo-β-lactamase producing serratia marcescens in a european university hospital. 201425008843
serratia marcescens arn, a phop-regulated locus necessary for polymyxin b resistance.polymyxins, which are increasingly being used to treat infections caused by multidrug-resistant bacteria, perform poorly against serratia marcescens. to investigate the underlying mechanisms, tn5 mutagenesis was performed and two mutants exhibiting increased polymyxin b (pb) susceptibility were isolated. the mutants were found to have tn5 inserted into the arnb and arnc genes. in other bacteria, arnb and arnc belong to the seven-gene arn operon, which is involved in lipopolysaccharide (lps) modi ...201424957827
genome sequence of meso-2,3-butanediol-producing strain serratia marcescens atcc 14041.serratia marcescens strain atcc 14041 was found to be an efficient meso-2,3-butanediol (meso-2,3-bd) producer from glucose and sucrose. here we present a 5.0-mb assembly of its genome. we have annotated 4 coding sequences (cdss) for meso-2,3-bd fermentation and 2 complete operons including 6 cdss for sucrose utilization.201424948764
[serratia marcescens osteomyelitis as the first manifestation of chronic granulomatous disease].chronic granulomatous disease is a rare, primary immunodeficiency disorder characterized by a defect in oxidative metabolism in phagocytes and recurrent bacterial and fungal infections. we report a case in a 2-month-old infant admitted with metacarpic osteomyelitis due to serratia marcescens. chronic granulomatous disease is rare but must be considered in cases of uncommon or atypical infection.201424935451
two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin.processivity refers to the ability of synthesizing, modifying and degrading enzymes to catalyse multiple successive cycles of reaction with polymeric substrates without disengaging from the substrates. since biomass polysaccharides, such as chitin and cellulose, often form recalcitrant crystalline regions, their degradation is highly dependent on the processivity of degrading enzymes. here we employ high-speed atomic force microscopy to directly visualize the movement of two processive glycoside ...201424894873
optimized production of serratia marcescens b742 mutants for preparing chitin from shrimp shells powders.to improve the deproteinization (dp) efficacy of shrimp shell powders (ssp) for preparing chitin, serratia marcescens b742 mutants were prepared using 2% diethyl sulfate (des), uv-irradiation, and/or microwave heating treatments. both single-stage and multi-stage mutations were investigated for optimizing s. marcescens b742 mutation conditions. under the optimized mutation conditions (2% des treatment for 30min plus successive 20min uv-irradiation), the protease and chitosanase activity produced ...201424879920
characterization of acetoin production in a budc gene disrupted mutant of serratia marcescens g12.the 2,3-butanediol (2,3-bd) dehydrogenase gene budc of serratia marcescens g12 was disrupted to construct the acetoin (ac) producing strain g12m. in shake-flask cultures, ac production was enhanced by increased concentrations of glucose or sodium acetate in g12m. in fed-batch fermentation, g12m produced 47.5 g/l ac along with 9.8 g/l 2,3-bd. the expression of the key enzymes for ac synthesis was further investigated. alpha-acetolactate synthase gene budb decreased its expression significantly in ...201424879481
bifunctional lanthanum phosphate substrates as novel adsorbents and biocatalyst supports for perchlorate removal.porous lanthanum phosphate substrates, obtained by an environmentally benign colloidal forming process employing methyl cellulose, are reported here as excellent adsorbents of perchlorate with >98% efficiency and with 100% reusability. additionally, the effectiveness of such substrates as biocatalyst supports that facilitate biofilm formation of perchlorate reducing microbes (serratia marcescens niist 5) is also demonstrated for the first time. the adsorption of perchlorate ions is attributed to ...201424872208
role of the phosphopantetheinyltransferase enzyme, pswp, in the biosynthesis of antimicrobial secondary metabolites by serratia marcescens db10.phosphopantetheinyltransferase (pptase) enzymes fulfil essential roles in primary and secondary metabolism in prokaryotes, archaea and eukaryotes. pptase enzymes catalyse the essential modification of the carrier protein domain of fatty acid synthases, polyketide synthases (pkss) and non-ribosomal peptide synthetases (nrpss). in bacteria and fungi, nrps and pks enzymes are often responsible for the biosynthesis of secondary metabolites with clinically relevant properties; these secondary metabol ...201424847000
multiple skin ulcers due to serratia marcescens in a immunocompetent patient.serratia marcescens is a species of gram negative bacillus, classified as a member of the enterobacteriaceae, mainly involved in opportunistic infections, particulary in the hospital environment. cutaneous infections have rarely reported in literature and are predominantly observed in elderly or in immunocompromised patients. the clinical manifestations of skin infections include granulomatous lesions, necrotizing fasciitis, nodules, cellulitis, ulcers, dermal abscesses. infections caused by s. ...201424819766
outbreak of meningitis due to serratia marcescens after spinal anaesthesia.this article describes an outbreak of meningitis caused by serratia marcescens in patients who had undergone spinal anaesthesia for caesarean section. bacterial meningitis was diagnosed in 12 of the 46 patients who underwent a caesarean section under spinal anaesthesia in a 75-bed private hospital between 6(th) and 14(th) march 2011. s. marcescens was isolated from samples taken from four prefilled syringes and one bag containing 5% dextrose with norepinephrine, suggesting that medications used ...201424814159
chitinase from a novel strain of serratia marcescens jpp1 for biocontrol of aflatoxin: molecular characterization and production optimization using response surface methodology.chitinase is one of the most important mycolytic enzymes with industrial significance, and produced by a number of organisms. a chitinase producing isolate serratia marcescens jpp1 was obtained from peanut hulls in jiangsu province, china, and exhibited antagonistic activity against aflatoxins. in this study, we describe the optimization of medium composition with increased production of chitinase for the selected bacteria using statistical methods: plackett-burman design was applied to find the ...201424812619
biochemical analysis of tssk, a core component of the bacterial type vi secretion system, reveals distinct oligomeric states of tssk and identifies a tssk-tssfg subcomplex.gram-negative bacteria use the type vi secretion system (t6ss) to inject toxic proteins into rival bacteria or eukaryotic cells. however, the mechanism of the t6ss is incompletely understood. in the present study, we investigated a conserved component of the t6ss, tssk, using the antibacterial t6ss of serratia marcescens as a model system. tssk was confirmed to be essential for effector secretion by the t6ss. the native protein, although not an integral membrane protein, appeared to localize to ...201424779861
new fatty acids from the red sea sponge mycale euplectellioides.chemical investigation of the red sea sponge mycale euplectellioides afforded two new compounds; hexacosa-(6z,10z)-dienoic acid methyl ester (1) and hexacosa-(6z,10z)-dienoic acid (2), along with two known compounds: icosa-(8z,11z)-dienoic acid methyl ester (3) and β-sitosterol (4). the structures were elucidated by the interpretation of their spectral data. the total methanol extract (tme) of the sponge exhibited potent antimicrobial activity against the different strains at a concentration of ...201424730698
qm/mm free-energy simulations of reaction in serratia marcescens chitinase b reveal the protonation state of asp142 and the critical role of tyr214.serratia marcescens chitinase b (chib), belonging to the glycosidase family 18 (gh18), catalyzes the hydrolysis of β-1,4-glycosidic bond, with retention of configuration, via an unusual substrate-assisted mechanism, in which the substrate itself acts as an intramolecular nucleophile. here, both elementary steps (glycosylation and deglycosylation) of the chib-catalyzed reaction are investigated by means of combined quantum mechanics/molecular mechanics (qm/mm) umbrella sampling molecular dynamics ...201424730355
outbreak of serratia marcescens bloodstream infections in patients receiving parenteral nutrition prepared by a compounding pharmacy.compounding pharmacies often prepare parenteral nutrition (pn) and must adhere to rigorous standards to avoid contamination of the sterile preparation. in march 2011, serratia marcescens bloodstream infections (bsis) were identified in 5 patients receiving pn from a single compounding pharmacy. an investigation was conducted to identify potential sources of contamination and prevent further infections.201424729502
structural characteristics of an insect group i chitinase, an enzyme indispensable to moulting.insects possess a greater number of chitinases than any other organisms. this work is the first report of unliganded and oligosaccharide-complexed crystal structures of the insect chitinase ofchti from ostrinia furnacalis, which is essential to moulting. the obtained crystal structures were solved at resolutions between 1.7 and 2.2 å. a structural comparison with other chitinases revealed that ofchti contains a long substrate-binding cleft similar to the bacterial chitinase smchib from serratia ...201424699639
molecular detection and analysis of a novel metalloprotease gene of entomopathogenic serratia marcescens strains in infected galleria mellonella.serratia marcescens strains isolated from entomopathogenic nematodes (rhabditis sp.) were examined for their pathogenicity and establishment in wax moth (galleria mellonella) larvae. all the serratia strains were potently pathogenic to g. mellonella larvae, leading to death within 48 h. the strains were shown to possess a metalloprotease gene encoding for a novel serralysin-like protein. rapid establishment of the bacteria in infected larvae was confirmed by specific polymerase chain reaction (p ...201424693978
serratia marcescens harbouring sme-type class a carbapenemases in canada and the presence of blasme on a novel genomic island, smargi1-1.an increasing prevalence since 2010 of serratia marcescens harbouring the ambler class a carbapenemase sme prompted us to further characterize these isolates.201424659751
serratia marcescens: an outbreak experience. 201424639671
predictors of positive cerebrospinal fluid cultures in infants with bacteremia.meningitis causes substantial morbidity and mortality in hospitalized infants. there is no consensus on the ability of blood cultures to predict results from cerebrospinal fluid (csf) cultures in hospitalized infants.201424632663
culture-dependent and culture-independent analyses reveal no prokaryotic community shifts or recovery of serratia marcescens in acropora palmata with white pox disease.recently, the etiological agent of white pox (wp) disease, also known as acroporid serratiosis, in the endangered coral acropora palmata is the enteric bacterium serratia marcescens with the source being localized sewage release onto coastal coral reef communities. here, we show that both culture-dependent and culture-independent approaches could not recover this bacterium from samples of tissue and mucus from a. palmata colonies affected by wp disease in the bahamas, or seawater collected adjac ...201424597458
properties and applications of undecylprodigiosin and other bacterial prodigiosins.the growing demand to fulfill the needs of present-day medicine in terms of novel effective molecules has lead to reexamining some of the old and known bacterial secondary metabolites. bacterial prodigiosins (prodiginines) have a long history of being re markable multipurpose compounds, best examined for their anticancer and antimalarial activities. production of prodigiosin in the most common producer strain serratia marcescens has been described in great detail. however, few reports have discu ...201424562326
the c-type lectin-like domain containing proteins clec-39 and clec-49 are crucial for caenorhabditis elegans immunity against serratia marcescens infection.caenorhabditis elegans exhibits protective immunity against a variety of fungal and bacterial pathogens. since c. elegans lacks an adaptive immune system, pathogen recognition is mediated entirely by innate immunity. to date, little is known about the involvement of pattern recognition receptors (prrs) in pathogen sensing as part of the c. elegans immunity. c-type lectin-like domain (ctld) containing proteins represent a superfamily of prrs. a large number of genes encoding for ctld proteins are ...201424534554
phenol, 2,4-bis(1,1-dimethylethyl) of marine bacterial origin inhibits quorum sensing mediated biofilm formation in the uropathogen serratia marcescens.intercellular communication in bacteria (quorum sensing, qs) is an important phenomenon in disease dissemination and pathogenesis, which controls biofilm formation also. this study reports the anti-qs and anti-biofilm efficacy of seaweed gracilaria gracilis associated vibrio alginolyticus g16 against serratia marcescens. purification and mass spectrometric analysis revealed the active principle as phenol, 2,4-bis(1,1-dimethylethyl) [pd]. pd affected the qs regulated virulence factor production i ...201425377484
endogenous serratia marcescens endophthalmitis.the purpose of this study was to describe a rare case of endogenous endophthalmitis associated with dental disease secondary to serratia marcescens in an hiv-negative individual.201425372197
discovery of a fluoroquinolone-resistant serratia marcescens clinical isolate without quinolone resistance-determining region mutations. 201425368830
pink breast milk: serratia marcescens colonization.background breast milk can turn pink with serratia marcescens colonization, this bacterium has been associated with several diseases and even death. it is seen most commonly in the intensive care settings. discoloration of the breast milk can lead to premature termination of nursing. we describe two cases of pink-colored breast milk in which s. marsescens was isolated from both the expressed breast milk. antimicrobial treatment was administered to the mothers. return to breastfeeding was success ...201425452881
enzyme processivity changes with the extent of recalcitrant polysaccharide degradation.glycoside hydrolases depolymerize polysaccharides. they can subtract single carbohydrate chains from polymer crystals and cleave glycosidic bonds without dissociating from the substrate after each catalytic event. this processivity is thought to conserve energy during polysaccharide degradation. herein, we compare the processivity of components of the chitinolytic machinery of serratia marcescens. the two processive chitinases chia and chib, the chib-w97a mutant, and the endochitinase chic were ...201425447535
analytical modeling and experimental characterization of chemotaxis in serratia marcescens.this paper presents a modeling and experimental framework to characterize the chemotaxis of serratia marcescens (s. marcescens) relying on two-dimensional and three-dimensional tracking of individual bacteria. previous studies mainly characterized bacterial chemotaxis based on population density analysis. instead, this study focuses on single-cell tracking and measuring the chemotactic drift velocity v(c) from the biased tumble rate of individual bacteria on exposure to a concentration gradient ...201425353826
interactions between the tropical sea anemone aiptasia pallida and serratia marcescens, an opportunistic pathogen of corals.coral reefs are under increasing stress caused by global and local environmental changes, which are thought to increase the susceptibility of corals to opportunistic pathogens. in the absence of an easily culturable model animal, the understanding of the mechanisms of disease progression in corals remains fairly limited. in the present study, we tested the susceptibility of the tropical sea anemone aiptasia pallida to an opportunistic coral pathogen (serratia marcescens). a. pallida was suscepti ...201424983533
detection of the prodigiosin biosynthesis protein (pigc) from serratia marcescens: development of a novel pcr assay. 201424974684
[endophytic bacterial community in the symptoms and symptomless tissues of hlb-affected citrus plant].to analyze the endophytic bacteria in symptoms and symptomless tissues, reveal dominant bacterial population which may interact with ca. las.201425345017
fatal sepsis in a child with thalassemia major due to serratia marcescens.one of the most important causes of mortality in thalassemic patients is infectious disease. thalassemic patients develop severe invasive infection caused by microorganisms that are rare in healthy individuals. we describe the case of a 13-year-old splenectomized boy who presented with septic shock and who died 36 h after admission, despite broad-spectrum antibiotics and aggressive supportive care. serratia marcescens was isolated from cultures of blood and tracheal aspirate. it is known that ra ...201425336004
act-10, a novel plasmid-encoded class c β-lactamase in a serratia marcescens isolate from china. 201425312010
genome assembly of serratia marcescens type strain atcc 13880.serratia marcescens atcc 13880 is the type strain of the species and a commonly used quality control strain. here, we present the annotated genome assembly of 5.13 mbp (59.8% g+c content) as submitted to ncbi under accession no. jovm00000000.201425291774
egg survival is reduced by grave-soil microbes in the carrion beetle, nicrophorus vespilloides.nicrophorus vespilloides eggs are deposited into the soil in close proximity to the decomposing vertebrate carcasses that these insects use as an obligate resource to rear their offspring. eggs in this environment potentially face significant risks from the bacteria that proliferate in the grave-soil environment following nutrient influx from the decomposing carcass. our aims in this paper are twofold: first, to examine the fitness effects of grave-soil bacteria to eggs, and second, to quantify ...201425260512
draft whole-genome sequence of serratia marcescens strain mcb, associated with oscheius sp. mcb (nematoda: rhabditidae) isolated from south africa.here we report on the draft genome sequence of serratia marcescens strain mcb associated with oscheius sp. mcb (nematoda: rhabditidae) isolated from south african soil. s. marcescens strain mcb has 5,304,212-bp genome size with 4,877 genes and a g+c content of 59.1%.201425237022
characterization of gram-negative psychrotrophic bacteria isolated from italian bulk tank milk.eighty psychrotrophic bacterial strains, isolated from different northwest italian bulk tank milks destined for grana padano cheese production, were identified by 16s rrna gene amplification and partial sequence analysis of the rpob gene. pseudomonas spp. were the most commonly occurring contaminants, p. fluorescens being the predominant isolated species, along with enterobacteriaceae, primarily serratia marcescens. rapd-pcr was used to study genetic variability and distinguish closely related s ...201425224662
draft genome sequence of a serratia marcescens strain isolated from a preterm neonatal blood sepsis patient at the royal infirmary, edinburgh, scotland, united kingdom.herein, we report the draft genome sequence for isolate ed-ngs-1015 of serratia marcescens, cultivated from a blood sample obtained from a neonatal sepsis patient at the royal infirmary in edinburgh, scotland, united kingdom.201425212627
potential transmission of pantoea spp. and serratia marcescens (enterobacteriales: enterobacteriaceae) to plants by lygus hesperus (hemiptera: miridae).lygus hesperus knight (hemiptera: miridae) is a key agricultural pest in the western united states. in a recent study, proteins from pantoea ananatis and serratia marcescens (enterobacteriales: enterobacteriaceae) were identified in diet that was stylet probed and fed on by l. hesperus adults. p. ananatis and s. marcescens are ubiquitous bacteria that infect a wide range of crops. the objective of our study was to determine whether l. hesperus transfer p. ananatis and s. marcescens to food subst ...201424665685
quality and safety of red blood cells stored in two additive solutions subjected to multiple room temperature exposures.many international standards state that red blood cell (rbc) products should be discarded if left out of controlled temperature storage for longer than 30 min to reduce the risk of bacterial growth and rbc loss of viability. this study aimed to verify whether repeated short-time exposures to room temperature (rt) influence rbcs quality and bacterial proliferation.201424730703
antimicrobial role of human meibomian lipids at the ocular surface.human meibomian lipids form the outermost lipid layer of the tear film and serve many important functions to maintain its integrity. although not investigated earlier, these lipids may have antimicrobial properties that help in strengthening the innate host defense of tears at the ocular surface. the aim of this study was to investigate the antimicrobial role of human meibomian lipids.201425316725
modulation of the epithelial sodium channel (enac) by bacterial metalloproteases and protease inhibitors.the serralysin family of metalloproteases is associated with the virulence of multiple gram-negative human pathogens, including pseudomonas aeruginosa and serratia marcescens. the serralysin proteases share highly conserved catalytic domains and show evolutionary similarity to the mammalian matrix metalloproteases. our previous studies demonstrated that alkaline protease (ap) from pseudomonas aeruginosa is capable of activating the epithelial sodium channel (enac), leading to an increase in sodi ...201424963801
replacing the axial ligand tyrosine 75 or its hydrogen bond partner histidine 83 minimally affects hemin acquisition by the hemophore hasap from pseudomonas aeruginosa.hemophores from pseudomonas aeruginosa (hasap), serratia marcescens (hasasm), and yersinia pestis (hasayp) bind hemin between two loops. one of the loops harbors conserved axial ligand tyr75 (y75 loop) in all three structures, whereas the second loop (h32 loop) contains axial ligand his32 in hasap and hasasm, but a noncoordinating gln32 in hasayp. binding of hemin to the y75 loop of hasap or hasasm causes a large rearrangement of the h32 loop that allows his32 coordination. the q32 loop in apo-h ...201424625274
microbial adherence to cosmetic contact lenses.to investigate whether cosmetic contact lenses (ccl) with surface pigments affect microbial adherence.201424440107
construction and characterization of a thermostable whole-cell chitinolytic enzyme using yeast surface display.to develop a novel yeast whole-cell biocatalyst by yeast surface display technology that can hydrolyze chitin, the chitinasec gene from serratia marcescens as1.1652 strain was cloned and subcloned into the yeast surface display plasmid pyd1, and the recombinant plasmid pyd1/smchic was electroporated into saccharomyces cerevisiae eby100 cell. aga2p-smchic fusion protein was expressed and anchored on the yeast cell surface by induction with galactose, which was verified by indirect immunofluoresce ...201424906465
studies on production and biological potential of prodigiosin by serratia marcescens.efficacy of serratia marcescens for pigment production and biological activity was investigated. natural substrates like sweet potato, mahua flower extract (madhuca latifolia l.), and sesam at different concentrations were taken. as a carbon source microorganism favored potato powder was followed by sesam and mannitol, and as nitrogen source casein hydrolysate was followed by yeast and malt extract. the effect of inorganic salts on pigment production was also studied. at final optimized composit ...201424781979
cyclic-amp inhibition of fimbriae and prodigiosin production by serratia marcescens is strain-dependent.the cyclic-nucleotide 3',5'-cyclic amp (camp) is an ancient and widespread regulatory molecule. previous studies have shown that fimbria production and secondary metabolite production are inhibited by camp in the prokaryote serratia marcescens. this study used genetic manipulations to test the strain specificity of camp-cyclic-amp receptor protein regulation of fimbria production and of the red pigment, prodigiosin. a surprising amount of variation was observed, as multicopy expression of the ca ...201424619531
pseudohemoptysis due to serratia marcescens. 201424186053
five-year prospective surveillance of nosocomial bloodstream infections in an estonian paediatric intensive care unit.few studies provide rates of nosocomial bloodstream infections (bsis) in mixed neonatal and paediatric intensive care units (picus).201424360408
late-onset neonatal sepsis, risk factors and interventions: an analysis of recurrent outbreaks of serratia marcescens, 2006-2011.between 2006 and 2011, 11 patients with serratia marcescens sepsis and 47 patients colonized due to the spread of various clones were observed. these recurrent clusters brought about interventions to reduce spread between patients.201424332914
[serratia marcescens cutaneous gumma and chronic septic granulomatosis]. 201424269059
antimicrobial effect and membrane-active mechanism of tea polyphenols against serratia marcescens.in this study, we investigated the antimicrobial effect of tea polyphenols (tp) against serratia marcescens and examined the related mechanism. morphology changes of s. marcescens were first observed by transmission electron microscopy after treatment with tp, which indicated that the primary inhibition action of tp was to damage the bacterial cell membranes. the permeability of the outer and inner membrane of s. marcescens dramatically increased after tp treatment, which caused severe disruptio ...201423979827
identification of a serratia marcescens virulence factor that promotes hemolymph bleeding in the silkworm, bombyx mori.injection of culture supernatant of serratia marcescens, a gram-negative bacterium pathogenic to a wide range of host animals including insects and mammals, into the hemolymph of silkworm (bombyx mori) larvae led to continuous flow of the hemolymph (blood of insects) from the injection site. the amount of hemolymph lost within 60 min reached 15-20% of the total larval weight. using a bioassay with live silkworms, we purified serralysin, a metalloprotease that requires divalent cations for its ac ...201424530643
draft genome sequence of serratia marcescens strain lct-sm166, a space flight strain with a specific carbon source utilization pattern.serratia marcescens has been detected in space habitats. to explore the influence of the space flight environment on this bacterium, we investigated the genome sequence of lct-sm166, which was isolated after space flight and has a specific carbon source utilization pattern.201424526644
mana is regulated by rssab signaling and promotes motility in serratia marcescens.serratia marcescens swarms on 0.8% lb agar at 30 °c but not at 37 °c. to understand the molecular mechanism regulating serratia swarming, transposon mutagenesis was performed to screen for mutants that swarmed at 37 °c. in one mutant, s. marcescens ww100, the transposon was inserted in the upstream region of mana, which encodes mannose-6-phosphate isomerase, a type i phosphomannose isomerase. the transcriptional and translational levels of mana were higher in s. marcescens ww100 than in the wild ...201424161484
endogenous serratia marcescens endophthalmitis: an atypical presentation. 201424177026
fulminant necrotizing fasciitis caused by serratia marcescens in an immunosuppressed host. 201422640271
community-acquired cutaneous ulcer in a child caused by serratia marcescens.serratia marcescens is a gram-negative, aerobic, motile bacillus belonging to the family enterobacteriaceae. s. marcescens has been implicated in different types of infections including urinary tract infection, septicaemia, meningitis and wound infections. very few cases of skin infections caused by this organism have been reported in the medical literature. s. marcescens is an important nosocomial pathogen but has rarely been implicated as a cause of community-acquired soft-tissue infections.201428663809
[status of health in west germany]. 20154622248
occurrence of autoimmune phenomena during the development of casein-induced amyloidosis in c3h and nzb-bl mice. 20154538553
hypertension detection by dentists. 20154510254
[bone changes in diabetics]. 20154453288
thyrotoxic periodic paralysis. 20154432182
veterans administration prosthetics center research report. 20154588224
[effect of hydrochlorothiazide on calcium concentration in serum and urine of patients with primary osteoporosis]. 20154409364
[familial type 3 hyperlipoproteinemia]. 20154365256
compound intussusception consisting of forward and retrograde components. 20154635530
[results of the surgical treatment of cancers of the transverse colon. apropos of 43 cases]. 20154652093
acedapsone in leprosy treatment: trial in 68 active cases in micronesia. 20154672994
cell response to surface stimulation. 20154508399
editorial: topics in radiology. 20154406740
[prof. dr med. tadeusz chorazak 70th anniversary]. 20154560869
[new abortion legislation]. 20154495979
on numerical integration of the hodgkin and huxley equations for a membrane action potential. 20154836889
microappendages on the ventral surface of the primitive streak chick embryo. 20154440636
drugs used in the management of tuberculosis. 3. primary tuberculostatic agents. 20154489152
[orgaization of local campaigns directed to the general public]. 20154429304
[sexuality of the unbalanced]. 20154676650
[biclonal gammapathy. (apropos of the isotopic study of a case observed in myeloma)]. 20154654968
the dalkon shield: tale of a tall. 20154442567
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