Publications

evidence for the induction of key components of the notch signaling pathway via deltamethrin and azamethiphos treatment in the sea louse caligus rogercresseyi.	the extensive use of organophosphates and pyrethroids in the aquaculture industry has negatively impacted parasite sensitivity to the delousing effects of these antiparasitics, especially among sea lice species. the notch signaling pathway is a positive regulator of abc transporter subfamily c expression and plays a key role in the generation and modulation of pesticide resistance. however, little is known about the molecular mechanisms behind pesticide resistance, partly due to the lack of geno ...	2016	27187362
wolbachia blocks viral genome replication early in infection without a transcriptional response by the endosymbiont or host small rna pathways.	the intracellular endosymbiotic bacterium wolbachia can protect insects against viral infection, and is being introduced into mosquito populations in the wild to block the transmission of arboviruses that infect humans and are a major public health concern. to investigate the mechanisms underlying this antiviral protection, we have developed a new model system combining wolbachia-infected drosophila melanogaster cell culture with the model mosquito-borne semliki forest virus (sfv; togaviridae, a ...	2016	27089431
zika virus strains potentially display different infectious profiles in human neural cells.	the recent zika virus (zikv) epidemic has highlighted the poor knowledge on its physiopathology. recent studies showed that zikv of the asian lineage, responsible for this international outbreak, causes neuropathology in vitro and in vivo. however, two african lineages exist and the virus is currently found circulating in africa. the original african strain was also suggested to be neurovirulent but its laboratory usage has been criticized due to its multiple passages. in this study, we compared ...	2016	27688094
pirna pathway is not required for antiviral defense in drosophila melanogaster.	since its discovery, rna interference has been identified as involved in many different cellular processes, and as a natural antiviral response in plants, nematodes, and insects. in insects, the small interfering rna (sirna) pathway is the major antiviral response. in recent years, the piwi-interacting rna (pirna) pathway also has been implicated in antiviral defense in mosquitoes infected with arboviruses. using drosophila melanogaster and an array of viruses that infect the fruit fly acutely o ...	2016	27357659
yellow fever virus capsid protein is a potent suppressor of rna silencing that binds double-stranded rna.	mosquito-borne flaviviruses, including yellow fever virus (yfv), zika virus (zikv), and west nile virus (wnv), profoundly affect human health. the successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. similar to other invertebrate animals and plants, the mosquito's rna silencing pathway comprises its primary antiviral defense. although a diverse range of plant and insect viruses has ...	2016	27849599
genome investigations of vector competence in aedes aegypti to inform novel arbovirus disease control approaches.	dengue (denv), yellow fever, chikungunya, and zika virus transmission to humans by a mosquito host is confounded by both intrinsic and extrinsic variables. besides virulence factors of the individual arboviruses, likelihood of virus transmission is subject to variability in the genome of the primary mosquito vector, aedes aegypti. the "vectorial capacity" of a. aegypti varies depending upon its density, biting rate, and survival rate, as well as its intrinsic ability to acquire, host and transmi ...	2016	27809220
climate change influences on the global potential distribution of the mosquito culex quinquefasciatus, vector of west nile virus and lymphatic filariasis.	rapid emergence of most vector-borne diseases (vbds) may be associated with range expansion of vector populations. culex quinquefasciatus say 1823 is a potential vector of west nile virus, saint louis encephalitis virus, and lymphatic filariasis. we estimated the potential distribution of cx. quinquefasciatus under both current and future climate conditions. the present potential distribution of cx. quinquefasciatus showed high suitability across low-latitude parts of the world, reflecting the c ...	2016	27695107
morphological abnormalities and cell death in the asian citrus psyllid (diaphorina citri) midgut associated with candidatus liberibacter asiaticus.	candidatus liberibacter asiaticus (clas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known as huanglongbing (hlb). clas is transmitted by the asian citrus psyllid (acp) diaphorina citri, in a circulative manner. two major barriers to transmission within the insect are the midgut and the salivary glands. we performed a thorough microscopic analysis within the insect midgut following exposure to clas-infected cit ...	2016	27630042
the strica homolog aacasps16 is involved in apoptosis in the yellow fever vector, aedes albopictus.	caspases are a family of cysteine proteases playing essential roles during apoptosis. seven caspases identified in drosophila were dronc, dredd, strica, dcp-1, decay, drice and damm. among them, strica is an insect-specific caspase containing a long serine- and threonine- rich prodomain, of which function is not yet well studied. here we identified a homolog of strica from aedes albopictus, named as aacasps16. aacasps16 encoded a protein containing a putative serine- and threonine-rich prodomain ...	2016	27351972
mosquito saliva increases endothelial permeability in the skin, immune cell migration, and dengue pathogenesis during antibody-dependent enhancement.	dengue remains the most prevalent arthropod-borne viral disease in humans. while probing for blood vessels, aedes aegypti and ae. albopictus mosquitoes transmit the four serotypes of dengue virus (denv1-4) by injecting virus-containing saliva into the skin. even though arthropod saliva is known to facilitate transmission and modulate host responses to other pathogens, the full impact of mosquito saliva on dengue pathogenesis is still not well understood. inoculating mice lacking the interferon-α ...	2016	27310141
a glance at subgenomic flavivirus rnas and micrornas in flavivirus infections.	the family flaviviridae comprises a wide variety of viruses that are distributed worldwide, some of which are associated with high rates of morbidity and mortality. there are neither vaccines nor antivirals for most flavivirus infections, reinforcing the importance of research on different aspects of the viral life cycle. during infection, cytoplasmic accumulation of rna fragments mainly originating from the 3' utrs, which have been designated subgenomic flavivirus rnas (sfrnas), has been detect ...	2016	27233361
infection pattern and transmission potential of chikungunya virus in two new world laboratory-adapted aedes aegypti strains.	chikungunya virus (chikv) is an emerging mosquito-borne virus belonging to the togaviridae, which is transmitted to humans by aedes aegypti and ae. albopictus. we describe the infection pattern of chikv in two new world ae. aegypti strains, hwe and orl. both mosquito strains were susceptible to the virus but showed different infection patterns in midguts and salivary glands. even though acquisition of a bloodmeal showed moderate levels of apoptosis in midgut tissue, there was no obvious addition ...	2016	27102548
endogenously-expressed nh2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands.	the circumsporozoite protein is the most abundant polypeptide expressed by sporozoites, the malaria parasite stage capable of infecting humans. sporozoite invasion of mosquito salivary glands prior to transmission is likely mediated by a receptor/ligand-like interaction of the parasites with the target tissues, and the amino (nh2)-terminal portion of csp is involved in this interaction but not the tsr region on the carboxyl (c)-terminus. peptides based on the nh2-terminal domain could compete wi ...	2016	26964736
genomic approaches for understanding dengue: insights from the virus, vector, and host.	the incidence and geographic range of dengue have increased dramatically in recent decades. climate change, rapid urbanization and increased global travel have facilitated the spread of both efficient mosquito vectors and the four dengue virus serotypes between population centers. at the same time, significant advances in genomics approaches have provided insights into host-pathogen interactions, immunogenetics, and viral evolution in both humans and mosquitoes. here, we review these advances an ...	2016	26931545
small rna profiling in dengue virus 2-infected aedes mosquito cells reveals viral pirnas and novel host mirnas.	in aedes mosquitoes, infections with arthropod-borne viruses (arboviruses) trigger or modulate the expression of various classes of viral and host-derived small rnas, including small interfering rnas (sirnas), piwi interacting rnas (pirnas), and micrornas (mirnas). viral sirnas are at the core of the antiviral rna interference machinery, one of the key pathways that limit virus replication in invertebrates. besides sirnas, aedes mosquitoes and cells derived from these insects produce arbovirus-d ...	2016	26914027
transstadial effects of bti on traits of aedes aegypti and infection with dengue virus.	most mosquito control efforts are primarily focused on reducing the adult population size mediated by reductions in the larval population, which should lower risk of disease transmission. although the aim of larviciding is to reduce larval abundance and thus recruitment of adults, nonlethal effects on adults are possible, including transstadial effects on phenotypes of adults such as survival and pathogen infection and transmission. in addition, the mortality induced by control efforts may act i ...	2016	26871951
inhibition of chikungunya virus replication by 1-[(2-methylbenzimidazol-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea(mbzm-n-ibt).	chikungunya virus (chikv) infection is one of the most challenging human arboviral infections with global significance and without any specific antiviral. in this investigation, 1-[(2-methylbenzimidazol-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea (mbzm-n-ibt) was synthesised as a molecular hybrid of 2-methyl benzimidazole and isatin-β-thiosemicarbazone and its anti-chikv property was evaluated. the release of infectious virus particles was calculated by plaque assay, expression profil ...	2016	26843462
apoptosis, autophagy and unfolded protein response pathways in arbovirus replication and pathogenesis.	arboviruses are pathogens that widely affect the health of people in different communities around the world. recently, a few successful approaches toward production of effective vaccines against some of these pathogens have been developed, but treatment and prevention of the resulting diseases remain a major health and research concern. the arbovirus infection and replication processes are complex, and many factors are involved in their regulation. apoptosis, autophagy and the unfolded protein r ...	2016	26781343
viral metagenomics on blood-feeding arthropods as a tool for human disease surveillance.	surveillance and monitoring of viral pathogens circulating in humans and wildlife, together with the identification of emerging infectious diseases (eids), are critical for the prediction of future disease outbreaks and epidemics at an early stage. it is advisable to sample a broad range of vertebrates and invertebrates at different temporospatial levels on a regular basis to detect possible candidate viruses at their natural source. however, virus surveillance systems can be expensive, costly i ...	2016	27775568
an updated checklist of mosquito species (diptera: culicidae) from madagascar.	an updated checklist of 235 mosquito species from madagascar is presented. the number of species has increased considerably compared to previous checklists, particularly the last published in 2003 (178 species). this annotated checklist provides concise information on endemism, taxonomic position, developmental stages, larval habitats, distribution, behavior, and vector-borne diseases potentially transmitted. the 235 species belong to 14 genera: aedeomyia (3 species), aedes (35 species), anophel ...	2016	27101839
the insect-specific palm creek virus modulates west nile virus infection in and transmission by australian mosquitoes.	insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. these viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. following the isolation of the first australian insect-specific flavivirus (isf), palm creek virus (pcv), we investigated routes of infection and transmission of this virus in key australian arbovirus vectors and its impact on replication ...	2016	27457250
development and validation of a quantitative, one-step, multiplex, real-time reverse transcriptase pcr assay for detection of dengue and chikungunya viruses.	dengue virus (denv) and chikungunya virus (chikv) are important human pathogens with common transmission vectors and similar clinical presentations. patient care may be impacted by the misdiagnosis of denv and chikv in areas where both viruses cocirculate. in this study, we have developed and validated a one-step multiplex reverse transcriptase pcr (rt-pcr) to simultaneously detect, quantify, and differentiate between four denv serotypes (pan-denv) and chikungunya virus. the assay uses taqman te ...	2016	27098955
vector competence of aedes aegypti and aedes polynesiensis populations from french polynesia for chikungunya virus.	from october 2014 to march 2015, french polynesia experienced for the first time a chikungunya outbreak. two aedes mosquitoes may have contributed to chikungunya virus (chikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito.	2016	27144888
vertical transmission of indian ocean lineage of chikungunya virus in aedes aegypti and aedes albopictus mosquitoes.	the re-emergence of chikungunya (chik) fever in thailand has been caused by a novel lineage of chikungunya virus (chikv) termed the indian ocean lineage (iol). the aedes albopictus mosquito is thought to be a primary vector of chik fever in thailand, whereas ae. aegypti acts as a secondary vector of the virus. the vertical transmission is believed to be a primary means to maintain chikv in nature and may be associated with an increased risk of outbreak. therefore, the goal of this study was to a ...	2016	27108077
membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying aedes-dengue virus interaction.	understanding the interaction between aedes vectors and dengue viruses (denv) has significant implications in determining the transmission dynamics of dengue. the absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured denv. data obtained from such studies may not reflect the natural human-mosquito transmission scenario. this study explored the potential of using me ...	2016	27083158
rapid detection strategies for the global threat of zika virus: current state, new hypotheses, and limitations.	the current scenario regarding the widespread zika virus (zikv) has resulted in numerous diagnostic studies, specifically in south america and in locations where there is frequent entry of travelers returning from zikv-affected areas, including pregnant women with or without clinical symptoms of zikv infection. the world health organization, who, announced that millions of cases of zikv are likely to occur in the usa in the near future. this situation has created an alarming public health emerge ...	2016	27822207
characterization of a novel murine model to study zika virus.	the mosquito-borne zika virus (zikv) is responsible for an explosive ongoing outbreak of febrile illness across the americas. zikv was previously thought to cause only a mild, flu-like illness, but during the current outbreak, an association with guillain-barré syndrome and microcephaly in neonates has been detected. a previous study showed that zikv requires murine adaptation to generate reproducible murine disease. in our study, a low-passage cambodian isolate caused disease and mortality in m ...	2016	27022155
epidemiology of emergent madariaga encephalitis in a region with endemic venezuelan equine encephalitis: initial host studies and human cross-sectional study in darien, panama.	neurotropic arboviral infections are an important cause of encephalitis. a zoonotic, vector-borne alphavirus, madariaga virus (madv; formerly known as south american eastern equine encephalitis virus), caused its first documented human outbreak in 2010 in darien, panama, where the genetically similar venezuelan equine encephalitis virus (veev) is endemic. we report the results of a seroprevalence survey of animals and humans, illustrating contrasting features of madv and veev ecology and epidemi ...	2016	27101567
the role of the membrane in the structure and biophysical robustness of the dengue virion envelope.	the dengue virion is surrounded by an envelope of membrane proteins surrounding a lipid bilayer. we have combined the cryoelectron microscopy structures of the membrane proteins (pdb: 3j27) with a lipid bilayer whose composition is based on lipidomics data for insect cell membranes, to obtain a near-atomic resolution computational model of the envelope of the dengue virion. a coarse-grained molecular dynamics simulation on the microsecond timescale enables analysis of key biophysical properties ...	2016	26833387
intra-epidemic evolutionary dynamics of a dengue virus type 1 population reveal mutant spectra that correlate with disease transmission.	dengue virus (denv) is currently the most prevalent mosquito-borne viral pathogen. denvs naturally exist as highly heterogeneous populations. even though the descriptions on denv diversity are plentiful, only a few studies have narrated the dynamics of intra-epidemic virus diversity at a fine scale. such accounts are important to decipher the reciprocal relationship between viral evolutionary dynamics and disease transmission that shape dengue epidemiology. in the current study, we present a mic ...	2016	26940650
zika virus and its implication in transfusion safety.		2016	26969782
transcriptome analysis of the midgut of the chinese oak silkworm antheraea pernyi infected with antheraea pernyi nucleopolyhedrovirus.	the antheraea pernyi nucleopolyhedrovirus (apnpv) is an exclusive pathogen of a. pernyi. the intense interactions between apnpv and a. pernyi cause a series of physiological and pathological changes to a. pernyi. however, no detailed report exists regarding the molecular mechanisms underlying the interactions between apnpv and a. pernyi. in this study, four cdna libraries of the a. pernyi midgut, including two apnpv-infected groups and two control groups, were constructed for transcriptomic anal ...	2016	27820844
prolonged shedding of chikungunya virus in semen and urine: a new perspective for diagnosis and implications for transmission.	we report the presence of chikungunya (chikv) rna in the blood, urine and semen during the acute phase of the disease in an adult with a dual infection with dengue virus type 3. the patient, a 25 yr-old man from salvador, brazil, reported a 6-day duration of high fever, arthralgia, myalgia, headache and photophobia.blood and semen specimens were positive for chikv in the first collected samples; semen and urine specimens were positive for chikv after 30 days of symptoms onset. denv-3 rna was pos ...	2016	27882301
genome sequence of dengue virus 3 from the pythium insidiosum transcriptomes.		2016	27379056
zika virus: new clinical syndromes and its emergence in the western hemisphere.	zika virus (zikv) had remained a relatively obscure flavivirus until a recent series of outbreaks accompanied by unexpectedly severe clinical complications brought this virus into the spotlight as causing an infection of global public health concern. in this review, we discuss the history and epidemiology of zikv infection, recent outbreaks in oceania and the emergence of zikv in the western hemisphere, newly ascribed complications of zikv infection, including guillain-barré syndrome and microce ...	2016	26962217
the global spread of zika virus: is public and media concern justified in regions currently unaffected?	zika virus, an aedes mosquito-borne flavivirus, is fast becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly among newborn infants in brazil.	2016	27093860
zika virus: the latest newcomer.	since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: west nile, influenza a, avian flu, dengue, chikungunya, sars, mers, ebola, and now zika, the latest newcomer. zika virus (zikv), a flavivirus transmitted by aedes mosquitoes, was identified in 1947 in a sentinel monkey in uganda, and later on in humans in nigeria. the virus was mainly confined to the african continent until it was detected in south-east asia the 1980's, t ...	2016	27148186
clinical and epidemiological characterization of laboratory-confirmed autochthonous cases of zika virus disease in mexico.	since 2014, autochthonous circulation of zika virus (zikv) in the americas was detected (easter island, chile). in may 2015, brazil confirmed autochthonous --transmission and in october of that year colombia reported their first cases. now more than 52 countries have reported cases, including mexico. to deal with this contingency in mexico, several surveillance systems, in addition to systems for vector-borne diseases were strengthened with the participation of all health institutions. also, th ...	2016	27158557
computational approach towards exploring potential anti-chikungunya activity of selected flavonoids.	chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya infection in humans. despite the widespread distribution of chikv, no antiviral medication or vaccine is available against this virus. therefore, it is crucial to find an effective compound to combat chikv. we aimed to predict the possible interactions between non-structural protein 3 (nsp) of chikv as one of the most important viral elements in chikv intracellular replication and 3 potential flavonoids using a comp ...	2016	27071308
zika virus infections imported from brazil to portugal, 2015.	zika virus is an emerging arbovirus transmitted by aedes sp. mosquitoes like the dengue and chikungunya viruses. zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. in 2007, an epidemic in micronesia initiated the turnover in the epidemiological history of zika virus and more recently, the potential association with congenital microcephaly cases in brazil 2015, still under investigation, led the world health organiz ...	2016	27134823
estimating risks of importation and local transmission of zika virus infection.	background. an international spread of zika virus (zikv) infection has attracted global attention. zikv is conveyed by a mosquito vector, aedes species, which also acts as the vector species of dengue and chikungunya viruses. methods. arrival time of zikv importation (i.e., the time at which the first imported case was diagnosed) in each imported country was collected from publicly available data sources. employing a survival analysis model in which the hazard is an inverse function of the effec ...	2016	27069825
identify-isolate-inform: a tool for initial detection and management of zika virus patients in the emergency department.	first isolated in 1947 from a monkey in the zika forest in uganda, and from mosquitoes in the same forest the following year, zika virus has gained international attention due to concerns for infection in pregnant women potentially causing fetal microcephaly. more than one million people have been infected since the appearance of the virus in brazil in 2015. approximately 80% of infected patients are asymptomatic. an association with microcephaly and other birth defects as well as guillain-barre ...	2016	27330653
recombinant dengue 2 virus ns3 helicase protein enhances antibody and t-cell response of purified inactivated vaccine.	dengue virus purified inactivated vaccines (piv) are highly immunogenic and protective over the short term, but may be poor at inducing cell-mediated immune responses and long-term protection. the dengue nonstructural protein 3 (ns3) is considered the main target for t-cell responses during viral infection. the amino (n)-terminal protease and the carboxy (c)-terminal helicase domains of denv-2 ns3 were expressed in e. coli and analyzed for their immune-potentiating capacity. mice were immunized ...	2016	27035715
zika virus: diagnostics for an emerging pandemic threat.	zika virus (zikv) is anaedesmosquito-borne flavivirus that emerged in brazil in 2015 and then rapidly spread throughout the tropical and subtropical americas. based on clinical criteria alone, zikv cannot be reliably distinguished from infections with other pathogens that cause an undifferentiated systemic febrile illness, including infections with two common arboviruses, dengue virus and chikungunya virus. this minireview details the methods that are available to diagnose zikv infection.	2016	26888897
maternal antibody-mediated disease enhancement in type i interferon-deficient mice leads to lethal disease associated with liver damage.	epidemiological studies have reported that most of the severe dengue cases occur upon a secondary heterologous infection. furthermore, babies born to dengue immune mothers are at greater risk of developing severe disease upon primary infection with a heterologous or homologous dengue virus (denv) serotype when maternal antibodies reach sub-neutralizing concentrations. these observations have been explained by the antibody mediated disease enhancement (ade) phenomenon whereby heterologous antibod ...	2016	27007501
nucleotide composition of the zika virus rna genome and its codon usage.	rna viruses have genomes with a distinct nucleotide composition and codon usage. we present the global characteristics of the rna genome of zika virus (zikv), an emerging pathogen within the flavivirus genus. zikv was first isolated in 1947 in uganda, caused a widespread epidemic in south and central america and the caribbean in 2015 and has recently been associated with microcephaly in newborns.	2016	27278486
pathogenesis of zika virus-associated embryopathy.	a strong causal association has become evident between zika virus (zikv) infection during pregnancy and the occurrence of fetal growth restriction, microcephaly and eye defects. circumstantial evidence is presented in this paper in support of the hypothesis that these effects, as well as the guillain-barré syndrome, are due to an endogenous form of hypervitaminosis a resulting from zikv infection-induced damage to the liver and the spillage of stored vitamin a compounds ("retinoids") into the ma ...	2016	27403405
long-term safety and immunogenicity of a tetravalent live-attenuated dengue vaccine and evaluation of a booster dose administered to healthy thai children.	we evaluated the safety and immunogenicity of two doses of a live-attenuated, tetravalent dengue virus vaccine (f17/pre formulation) and a booster dose in a dengue endemic setting in two studies. seven children (7- to 8-year-olds) were followed for 1 year after dose 2 and then given a booster dose (f17/pre formulation), and followed for four more years (child study). in the infant study, 49 2-year-olds, vaccinated as infants, were followed for approximately 3.5 years after dose 2 and then given ...	2016	27022153
scoping review of the zika virus literature.	the global primary literature on zika virus (zikv) (n = 233 studies and reports, up to march 1, 2016) has been compiled using a scoping review methodology to systematically identify and characterise the literature underpinning this broad topic using methods that are documented, updateable and reproducible. our results indicate that more than half the primary literature on zikv has been published since 2011. the articles mainly covered three topic categories: epidemiology of zikv (surveillance an ...	2016	27244249
quantifying the epidemiological impact of vector control on dengue.		2016	27227829
a pan-dengue virus reverse transcription-insulated isothermal pcr assay intended for point-of-need diagnosis of dengue virus infection by use of the pockit nucleic acid analyzer.	dengue virus (denv) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal pcr (rt-iipcr) method for the detection of all four serotypes of denv in clinical samples. the diagnostic performance of the newly established pan-denv rt-iipc ...	2016	27030492
determinants of arbovirus vertical transmission in mosquitoes.	vertical transmission (vt) and horizontal transmission (ht) of pathogens refer to parental and non-parental chains of host-to-host transmission. combining ht with vt enlarges considerably the range of ecological conditions in which a pathogen can persist, but the factors governing the relative frequency of each transmission mode are poorly understood for pathogens with mixed-mode transmission. elucidating these factors is particularly important for understanding the epidemiology of arthropod-bor ...	2016	27171170
physicians, primary caregivers and topical repellent: all under-utilised resources in stopping dengue virus transmission in affected households.	primary health care facilities frequently manage dengue cases on an ambulatory basis for the duration of the patient's illness. there is a great opportunity for specific messaging, aimed to reduce dengue virus (denv) transmission in and around the home, to be directly targeted toward this high-risk ambulatory patient group, as part of an integrated approach to dengue management. the extent however, to which physicians understand, and can themselves effectively communicate strategies to stop foca ...	2016	27164168
coeloconic sensilla on the antennae of the yellow fever mosquito, aedes aegypti (l.).		2016	5026452
aphicidal activity of illicium verum fruit extracts and their effects on the acetylcholinesterase and glutathione s-transferases activities in myzus persicae (hemiptera: aphididae).	this study aims to explore the aphicidal activity and underlying mechanism of illicium verum hook. f. that is used as both food and medicine. the contact toxicity of the extracts from i. verum fruit with methyl alcohol (ma), ethyl acetate (ea), and petroleum ether (pe) against myzus persicae (sulzer), and the activities of acetylcholinesterase (ache) and glutathione s-transferases (gsts) of m. persicae after contact treatment were tested. the results showed that ma, ea, and pe extracts of 1.000 ...	2016	26826651
identification of the temperature induced larvicidal efficacy of agave angustifolia against aedes, culex, and anopheles larvae.	synthetic insecticides are generally employed to control the mosquito population. however, their injudicious over usage and non-biodegradability are associated with many adverse effects on the environment and mosquitoes. the application of environment-friendly mosquitocidals might be an alternate to overcome these issues. in this study, we found that organic or aqueous extracts of agave angustifolia leaves exhibited a strong larvicidal activity (ld50 28.27 μg/ml) against aedes aegypti, culex qui ...	2016	26793700
mosquitocidal effect of glycosmis pentaphylla leaf extracts against three mosquito species (diptera: culicidae).	the resistance status of malaria vectors to different classes of insecticides used for public health has raised concern for vector control programmes. alternative compounds to supplement the existing tools are important to be searched to overcome the existing resistance and persistence of pesticides in vectors and the environment respectively. the mosquitocidal effects of glycosmis pentaphylla using different solvents of acetone, methanol, chloroform and ethyl acetate extracts against three medi ...	2016	27391146
leucokinin mimetic elicits aversive behavior in mosquito aedes aegypti (l.) and inhibits the sugar taste neuron.	insect kinins (leucokinins) are multifunctional peptides acting as neurohormones and neurotransmitters. in females of the mosquito vector aedes aegypti (l.), aedeskinins are known to stimulate fluid secretion from the renal organs (malpighian tubules) and hindgut contractions by activating a g protein-coupled kinin receptor designated "aedae-kr." we used protease-resistant kinin analogs 1728, 1729, and 1460 to evaluate their effects on sucrose perception and feeding behavior. in no-choice feedin ...	2016	27274056
evaluation of sublethal effects of ipomoea cairica linn. extract on life history traits of dengue vectors.	plant derived insecticides have considerable potential for mosquito control because these products are safer than conventional insecticides. this study aimed to investigate sublethal activities of ipomoea carica or railway creeper crude acethonilic extract against life history trait of dengue vectors, aedes albopictus and aedes aegypti. the late third instar larvae of ae. albopictus and ae. aegypti were exposed to a sublethal dose at lc50 and larvae that survived were further cultured. overall, ...	2016	27253746
a novel xenomonitoring technique using mosquito excreta/feces for the detection of filarial parasites and malaria.	given the continued successes of the world's lymphatic filariasis (lf) elimination programs and the growing successes of many malaria elimination efforts, the necessity of low cost tools and methodologies applicable to long-term disease surveillance is greater than ever before. as many countries reach the end of their lf mass drug administration programs and a growing number of countries realize unprecedented successes in their malaria intervention efforts, the need for practical molecular xenom ...	2016	27096156
insect transformation with piggybac: getting the number of injections just right.	the insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggybac is the transposable element most commonly utilized for insect germline transformation. the described efficiency of this process is variable in the published literature, and a comprehensive review of transformation efficiency in insects is lacking. this study compared and contrasted ...	2016	27027400
fabrication of metal nanoparticles from fungi and metal salts: scope and application.	fungi secrete enzymes and proteins as reducing agents which can be used for the synthesis of metal nanoparticles from metal salts. large-scale production of nanoparticles from diverse fungal strains has great potential since they can be grown even in vitro. in recent years, various approaches have been made to maximize the yield of nanoparticles of varying shape, size, and stability. they have been characterized by thermogravimetric analysis, x-ray diffractometry, sem/tem, zeta potential measure ...	2016	26909778
development of non-defective recombinant densovirus vectors for microrna delivery in the invasive vector mosquito, aedes albopictus.	we previously reported that mosquito densoviruses (mdvs) are potential vectors for delivering foreign nucleic acids into mosquito cells. however, considering existing expression strategies, recombinant viruses would inevitably become replication-defective viruses and lose their ability for secondary transmission. the packaging limitations of the virion represent a barrier for the development of mdvs for viral paratransgenesis or as high-efficiency bioinsecticides. herein, we report the developme ...	2016	26879823
an evidence-based review on medicinal plants used as insecticide and insect repellent in traditional iranian medicine.	insects can be the cause of major ecological problems; they can transmit microbes and parasites that affect humans, and damage food crops, trees, and homes. the total economic cost of insect-related damage and disease is immeasurable. in traditional iranian medicine (tim), several medicinal plants have been identified as insecticides or insect repellents, but many of them are still unknown. therefore, the aim of this study was to review the insecticidal or insect repellent activity of certain me ...	2016	27186389
large-scale anopheles arabiensis egg quantification methods for mass-rearing operations.	the success of the sterile insect technique relies, among other things, on the continuous release of over flooding numbers of sexually competitive sterile males into the target area. to produce sufficiently large quantities of sterile males, rearing protocols need to be optimized including the development and validation of a standardized egg quantification method.	2016	26852018
identification of male- and female-specific olfaction genes in antennae of the oriental fruit fly (bactrocera dorsalis).	the oriental fruit fly (bactrocera dorsalis) is a species of tephritid fruit fly, endemic to southeast asia but also introduced to many regions of the us, and it is one of the major pest species with a broad host range of cultivated and wild fruits. although males of b. dorsalis respond strongly to methyl eugenol and this is used for monitoring and estimating populations, the molecular mechanism of the oriental fruit fly olfaction has not been elucidated yet. therefore, in this project, using ne ...	2016	26845547
methanolic extract of agerantum conyzoides exhibited toxicity and growth disruption activities against anopheles gambiae sensu stricto and anopheles arabiensis larvae.	vector control remains the mainstay to effective malaria management. the negative implications following persistent application of synthetic insecticides geared towards regulation of mosquito populations have necessitated prospection for ecofriendly effective chemistries. plant-derived compounds have the potential to control malaria-transmitting mosquito populations. previously, agerantum conyzoides extracts have demonstrated toxicity effects on disease-transmitting mosquitoes. however, their ef ...	2016	27876055
identification of genes for engineering the male germline of aedes aegypti and ceratitis capitata.	synthetic biology approaches are promising new strategies for control of pest insects that transmit disease and cause agricultural damage. these strategies require characterised modular components that can direct appropriate expression of effector sequences, with components conserved across species being particularly useful. the goal of this study was to identify genes from which new potential components could be derived for manipulation of the male germline in two major pest species, the mosqui ...	2016	27871244
does fluoride influence oviposition of anopheles stephensi in stored water habitats in an urban setting?	the physico-chemical characteristics of lentic aquatic habitats greatly influence mosquito species in selecting suitable oviposition sites; immature development, pupation and adult emergence, therefore are considerations for their preferred ecological niche. correlating water quality parameters with mosquito breeding, as well as immature vector density, are useful for vector control operations in identifying and targeting potential breeding habitats.	2016	27829419
identification and characterization of micrornas expressed in the african malaria vector anopheles funestus life stages using high throughput sequencing.	over the past several years, thousands of micrornas (mirnas) have been identified in the genomes of various insects through cloning and sequencing or even by computational prediction. however, the number of mirnas identified in anopheline species is low and little is known about their role. the mosquito anopheles funestus is one of the dominant malaria vectors in africa, which infects and kills millions of people every year. therefore, small rna molecules isolated from the four life stages (eggs ...	2016	27825380
vorticella sp: prospective mosquito biocontrol agent.	considering the disadvantages of chemical insecticides, we aimed to evaluate vorticella parasites for control of mosquito larvae of anopheles stephensi and aedes aegypti at different larval stages.	2016	28032113
laboratory evaluation of temephos against anopheles stephensi and culex pipiens larvae in iran.	malaria is still a health problem in iran. there are several vector control activities, including indoor residual spraying, using insecticide treated nets and larviciding including temephos. in addition nuisance mosquitos are prevalent in the urban areas. so that evaluation of this species to larvicide will provide a clue for management of vector control activities.	2016	28032103
guy1 confers complete female lethality and is a strong candidate for a male-determining factor in anopheles stephensi.	despite their importance in sexual differentiation and reproduction, y chromosome genes are rarely described because they reside in repeat-rich regions that are difficult to study. here, we show that guy1, a unique y chromosome gene of a major urban malaria mosquito anopheles stephensi, confers 100% female lethality when placed on the autosomes. we show that the small guy1 protein (56 amino acids in length) causes female lethality and that males carrying the transgene are reproductively more com ...	2016	27644420
effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout aedes aegypti lifespan.	mosquito midgut microbiota is a key component of vector competence, as gut bacteria can disturb pathogen development. in this study, we addressed the microbiota composition of aedes aegypti during its lifespan, under field conditions. we also investigated the possible effects of environment, dietary regime and ageing on the gut community composition. we employed culture independent and dependent approaches to characterise vector microbiota. there was evidence of a lifelong stable core microbiota ...	2016	27580348
seminal fluid protein genes of the brown planthopper, nilaparvata lugens.	seminal fluid proteins (sfps) are produced mainly in the accessory gland of male insects and transferred to females during mating, in which they induce numerous physiological and post-mating behavioral changes. the brown plant hopper (bph), nilaparvata lugens, is an economically important hemipterous pest of rice. the behavior and physiology of the female of this species is significantly altered by mating. sfps in hemipteran species are still unclear.	2016	27538518
an improved mosquito electrocuting trap that safely reproduces epidemiologically relevant metrics of mosquito human-feeding behaviours as determined by human landing catch.	reliable quantification of mosquito host-seeking behaviours is required to determine the efficacy of vector control methods. for malaria, the gold standard approach remains the risky human landing catch (hlc). here compare the performance of an improved prototype of the mosquito electrocuting grid trap (met) as a safer alternative with hlc for measuring malaria vector behaviour in dar es salaam, tanzania.	2016	27618941
comparative performance of transcriptome assembly methods for non-model organisms.	the technological revolution in next-generation sequencing has brought unprecedented opportunities to study any organism of interest at the genomic or transcriptomic level. transcriptome assembly is a crucial first step for studying the molecular basis of phenotypes of interest using rna-sequencing (rna-seq). however, the optimal strategy for assembling vast amounts of short rna-seq reads remains unresolved, especially for organisms without a sequenced genome. this study compared four transcript ...	2016	27464550
characterization of a midgut mucin-like glycoconjugate of lutzomyia longipalpis with a potential role in leishmania attachment.	leishmania parasites are transmitted by phlebotomine sand flies and a crucial step in their life-cycle is the binding to the sand fly midgut. laboratory studies on sand fly competence to leishmania parasites suggest that the sand flies fall into two groups: several species are termed "specific/restricted" vectors that support the development of one leishmania species only, while the others belong to so-called "permissive" vectors susceptible to a wide range of leishmania species. in a previous s ...	2016	27457627
purification and characterization of a novel kazal-type trypsin inhibitor from the leech of hirudinaria manillensis.	kazal-type serine proteinase inhibitors are found in a large number of living organisms and play crucial roles in various biological and physiological processes. although some kazal-type serine protease inhibitors have been identified in leeches, none has been reported from hirudinaria manillensis, which is a medically important leech. in this study, a novel kazal-type trypsin inhibitor was isolated from leech h. manillensis, purified and named as bdellin-hm based on the sequence similarity with ...	2016	27455325
the queenslandensis and the type form of the dengue fever mosquito (aedes aegypti l.) are genomically indistinguishable.	the mosquito aedes aegypti (l.) is a major vector of viral diseases like dengue fever, zika and chikungunya. aedes aegypti exhibits high morphological and behavioral variation, some of which is thought to be of epidemiological significance. globally distributed domestic ae. aegypti have often been grouped into (i) the very pale variety queenslandensis and (ii) the type form. because the two color forms co-occur across most of their range, there is interest in understanding how freely they interb ...	2016	27806047
discovery of point mutations in the voltage-gated sodium channel from african aedes aegypti populations: potential phylogenetic reasons for gene introgression.	yellow fever is endemic in some countries in africa, and aedes aegpyti is one of the most important vectors implicated in the outbreak. the mapping of the nation-wide distribution and the detection of insecticide resistance of vector mosquitoes will provide the beneficial information for forecasting of dengue and yellow fever outbreaks and effective control measures.	2016	27304430
detection and perception of generic host volatiles by mosquitoes modulate host preference: context dependence of (r)-1-octen-3-ol.	natural selection favours a restricted host breadth in disease vector mosquitoes, indicating that there is an adaptive value associated with maintaining plasticity in host preference. one mechanism to maintain such plasticity is via the detection of generic cues by conserved peripheral olfactory pathways, which when perceived in different host odour contexts enable the identification of and discrimination among potential host species. here, we show that the context of an odour cue shapes host pe ...	2016	28018630
larval nutritional stress affects vector life history traits and human malaria transmission.	exposure to stress during an insect's larval development can have carry-over effects on adult life history traits and susceptibility to pathogens. we investigated the effects of larval nutritional stress for the first time using field mosquito vectors and malaria parasites. in contrast to previous studies, we show that larval nutritional stress may affect human to mosquito transmission antagonistically: nutritionally deprived larvae showed lower parasite prevalence for only one gametocyte carrie ...	2016	27827429
multiple insecticide resistance in the malaria vector anopheles funestus from northern cameroon is mediated by metabolic resistance alongside potential target site insensitivity mutations.	despite the recent progress in establishing the patterns of insecticide resistance in the major malaria vector anopheles funestus, central african populations of this species remain largely uncharacterised. to bridge this important gap and facilitate the implementation of suitable control strategies against this vector, we characterised the resistance patterns of an. funestus population from northern cameroon.	2016	27723825
tyrosine hydroxylase is crucial for maintaining pupal tanning and immunity in anopheles sinensis.	tyrosine hydroxylase (th), the initial enzyme in the melanin pathway, catalyzes tyrosine conversion into dopa. although expression and regulation of th have been shown to affect cuticle pigmentation in insects, no direct functional studies to date have focused on the specific physiological processes involving the enzyme during mosquito development. in the current study, silencing of asth during the time period of continuous high expression in anopheles sinensis pupae led to significant impairmen ...	2016	27416870
seeing is believing: the nocturnal malarial mosquito anopheles coluzzii responds to visual host-cues when odour indicates a host is nearby.	the immediate aim of our study was to analyse the behaviour of the malarial mosquito anopheles coluzzii (an. gambiae species complex) near a human host with the ultimate aim of contributing to our fundamental understanding of mosquito host-seeking behaviour and the overall aim of identifying behaviours that could be exploited to enhance sampling and control strategies.	2016	27260254
attractiveness of volatiles from different body parts to the malaria mosquito anopheles coluzzii is affected by deodorant compounds.	mosquitoes display biting preferences among different sites of the human body. in addition to height or convection currents, body odour may play a role in the selection of these biting sites. previous studies have shown that skin emanations are important host-finding cues for mosquitoes. in this study, skin emanations were collected from armpits, hands and feet; the volatile profiles were analysed and tested for their attractiveness to the malaria mosquito anopheles coluzzii. skin emanations col ...	2016	27251017
wolbachia infections in natural anopheles populations affect egg laying and negatively correlate with plasmodium development.	the maternally inherited alpha-proteobacterium wolbachia has been proposed as a tool to block transmission of devastating mosquito-borne infectious diseases like dengue and malaria. here we study the reproductive manipulations induced by a recently identified wolbachia strain that stably infects natural mosquito populations of a major malaria vector, anopheles coluzzii, in burkina faso. we determine that these infections significantly accelerate egg laying but do not induce cytoplasmic incompati ...	2016	27243367
the larvicidal effects of black pepper (piper nigrum l.) and piperine against insecticide resistant and susceptible strains of anopheles malaria vector mosquitoes.	insecticide resistance carries the potential to undermine the efficacy of insecticide based malaria vector control strategies. therefore, there is an urgent need for new insecticidal compounds. black pepper (dried fruit from the vine, piper nigrum), used as a food additive and spice, and its principal alkaloid piperine, have previously been shown to have larvicidal properties. the aim of this study was to investigate the larvicidal effects of ground black pepper and piperine against third and fo ...	2016	27117913
comparison of irradiation and wolbachia based approaches for sterile-male strategies targeting aedes albopictus.	the global expansion of aedes albopictus together with the absence of vaccines for most of the arboviruses transmitted by this mosquito has stimulated the development of sterile-male strategies aiming at controlling disease transmission through the suppression of natural vector populations. in this context, two environmentally friendly control strategies, namely the sterile insect technique (sit) and the wolbachia-based incompatible insect technique (iit) are currently being developed in several ...	2016	26765951
pyrosequencing the midgut transcriptome of the banana weevil cosmopolites sordidus (germar) (coleoptera: curculionidae) reveals multiple protease-like transcripts.	the banana weevil cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. in spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. in the present study, we characterized the midgut transcriptome of c. sordidus using massive 454-pyrosequencing. we generated over 590,000 sequencing reads that assembled into 30,840 contigs with more than 400 bp, representing a sig ...	2016	26949943
proteomic profile of the bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole.	benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against sclerotinia sclerotiorum, ditylenchus destructor and bradysia odoriphaga. however, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. here, we employed itraq-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of b. odoriphaga. in response to benzothiazole, 92 of ...	2016	27883048
declining prevalence of disease vectors under climate change.	more than half of the world population is at risk of vector-borne diseases including dengue fever, chikungunya, zika, yellow fever, leishmaniasis, chagas disease, and malaria, with highest incidences in tropical regions. in ecuador, vector-borne diseases are present from coastal and amazonian regions to the andes mountains; however, a detailed characterization of the distribution of their vectors has never been carried out. we estimate the distribution of 14 vectors of the above vector-borne dis ...	2016	27982119
bacterial diversity of the american sand fly lutzomyia intermedia using high-throughput metagenomic sequencing.	parasites of the genus leishmania cause a broad spectrum of diseases, collectively known as leishmaniasis, in humans worldwide. american cutaneous leishmaniasis is a neglected disease transmitted by sand fly vectors including lutzomyia intermedia, a proven vector. the female sand fly can acquire or deliver leishmania spp. parasites while feeding on a blood meal, which is required for nutrition, egg development and survival. the microbiota composition and abundance varies by food source, life sta ...	2016	27581188
"the environment is everything that isn't me": molecular mechanisms and evolutionary dynamics of insect clocks in variable surroundings.	circadian rhythms are oscillations in behavior, metabolism and physiology that have a period close to 24 h. these rhythms are controlled by an internal pacemaker that evolved under strong selective pressures imposed by environmental cyclical changes, mainly of light and temperature. the molecular nature of the circadian pacemaker was extensively studied in a number of organisms under controlled laboratory conditions. but although these studies were fundamental to our understanding of the circadi ...	2016	26793115
reproductive incompatibility involving senegalese aedes aegypti (l) is associated with chromosome rearrangements.	aedes aegypti, the primary vector of dengue, yellow fever and zika flaviviruses, consists of at least two subspecies. aedes aegypti (aaa) is light in color, has pale scales on the first abdominal tergite, oviposits in artificial containers, and preferentially feeds on humans. aedes aegypti formosus (aaf), has a dark cuticle, is restricted to sub-saharan africa, has no pale scales on the first abdominal tergite and frequently oviposits in natural containers. scale patterns correlate with cuticle ...	2016	27105225
piwis go viral: arbovirus-derived pirnas in vector mosquitoes.	vector mosquitoes are responsible for transmission of the majority of arthropod-borne (arbo-) viruses. virus replication in these vectors needs to be sufficiently high to permit efficient virus transfer to vertebrate hosts. the mosquito immune response therefore is a key determinant for arbovirus transmission. mosquito antiviral immunity is primarily mediated by the small interfering rna pathway. besides this well-established antiviral machinery, the piwi-interacting rna (pirna) pathway processe ...	2016	28033427
viral rna silencing suppression: the enigma of bunyavirus nss proteins.	the bunyaviridae is a family of arboviruses including both plant- and vertebrate-infecting representatives. the tospovirus genus accommodates plant-infecting bunyaviruses, which not only replicate in their plant host, but also in their insect thrips vector during persistent propagative transmission. for this reason, they are generally assumed to encounter antiviral rna silencing in plants and insects. here we present an overview on how tospovirus nonstructural nss protein counteracts antiviral r ...	2016	27455310
biotype characterization, developmental profiling, insecticide response and binding property of bemisia tabaci chemosensory proteins: role of csp in insect defense.	chemosensory proteins (csps) are believed to play a key role in the chemosensory process in insects. sequencing genomic dna and rna encoding csp1, csp2 and csp3 in the sweet potato whitefly bemisia tabaci showed strong variation between b and q biotypes. analyzing csp-rna levels showed not only biotype, but also age and developmental stage-specific expression. interestingly, applying neonicotinoid thiamethoxam insecticide using twenty-five different dose/time treatments in b and q young adults s ...	2016	27167733
molecular diversity between salivary proteins from new world and old world sand flies with emphasis on bichromomyia olmeca, the sand fly vector of leishmania mexicana in mesoamerica.	sand fly saliva has been shown to have proteins with potent biological activities, salivary proteins that can be used as biomarkers of vector exposure, and salivary proteins that are candidate vaccines against different forms of leishmaniasis. sand fly salivary gland transcriptomic approach has contributed significantly to the identification and characterization of many of these salivary proteins from important leishmania vectors; however, sand fly vectors in some regions of the world are still ...	2016	27409591